Full-automatic immunochromatographic instrument light source reading device and working method

Abstract

The application discloses a full-automatic immunochromatographic instrument light source reading device and a working method thereof. The device comprises light source receiving plates, first optical fibers, first optical fiber joints, first optical fiber joint female seats, optical filters, second optical fiber joint female seats, second optical fiber joints, second optical fibers and third optical fiber joints which are sequentially connected. One end of the light source receiving plate is bent downward to form a limiting plate. A first optical fiber through hole is arranged on the upper portion of the limiting plate. A recess for placing a cover plate is arranged on the upper surface of the light source receiving plate. An optical fiber guide groove is formed by recessing a side close to the first optical fiber through hole in the recess downward. The optical fiber guide groove is communicated with the first optical fiber through hole on one side. A second optical fiber through hole is arranged on the wall surface on the other side. A boss is formed by protruding the center of the part close to the optical fiber guide groove in the recess upward. A third optical fiber through hole is arranged in the boss. The third optical fiber through hole is communicated with the second optical fiber through hole. The first optical fiber sequentially passes through the first optical fiber through hole, the second optical fiber through hole and the third optical fiber through hole and is exposed from the third optical fiber through hole.

Description

Technical Field

[0001] This invention relates to the field of immunochromatography technology, and in particular to a fully automated immunochromatographic analyzer light source reading device and its working method. Background Technology

[0002] The fully automated immunochromatographic analyzer is an intelligent testing device integrating sample processing, reaction incubation, and result interpretation, specifically designed for immunochromatographic techniques (such as colloidal gold and fluorescent labeling). It automatically completes the entire process of sample addition, incubation, elution, and signal acquisition, supporting multiple sample types including whole blood, serum, and urine. It can process dozens of samples in a single batch, improving detection efficiency by more than three times compared to manual operation. The instrument incorporates a high-precision optical sensor that accurately identifies T / C line signals with a CV value <5%, ensuring reliable results. Equipped with a touchscreen interface and human-computer interaction system, it supports LIS / HIS data integration, enabling real-time uploading and traceability management of test results. It is widely used for detecting cardiac markers (such as cTnI and NT-proBNP), inflammatory factors (PCT and CRP), infectious diseases (influenza and COVID-19), and pregnancy-related indicators, providing quantitative / semi-quantitative reports within 15 minutes, offering an efficient and standardized solution for rapid clinical diagnosis.

[0003] Currently, most fully automated immunochromatographic analyzers use LED (visible / near-infrared) or laser light sources to ensure light intensity fluctuations are <1%. In fluorescent labeling systems, specific wavelength LEDs (such as 365nm, 475nm, 525nm, 770nm near-infrared) or laser diodes are used as excitation sources, with filters used to read the emitted fluorescence signals. Colloidal gold / chromogenic labeling systems use white LEDs or cold light sources to scan the reflected light intensity, and absorbance is analyzed using RGB spectrum analysis. Fully automated immunochromatographic analyzers primarily use photomultiplier tubes (PMTs), avalanche photodiodes (APDs), or high-sensitivity CCD / CMOS sensors to convert light signals into current signals, analyze their photometric values, and then, through modeling, derive the corresponding detection value (the content of a certain substance in the sample).

[0004] However, current light source testing for fully automated immunochromatographic analyzers mostly focuses on the stability of the optical path detection system (such as background light intensity and signal stability). There are currently no devices on the market specifically designed to test the accuracy of the light source in fully automated immunochromatographic analyzers. Therefore, to test the light source parameters of a fully automated immunochromatographic analyzer, the light source must be disassembled and tested separately, which not only easily damages the instrument but also increases labor and time costs. Therefore, there is an urgent need in this field to develop a device dedicated to detecting the core light source parameters (peak height, half-bandwidth, and center wavelength) of the optical path system in fully automated immunochromatographic analyzers. Summary of the Invention

[0005] The purpose of this invention is to provide a fully automated immunochromatographic analyzer light source reading device and its working method.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A fully automated immunochromatographic analyzer light source reading device includes a light source receiving board, a first optical fiber, a first optical fiber connector, a first optical fiber connector socket, a filter, a second optical fiber connector socket, a second optical fiber connector, a second optical fiber, and a third optical fiber connector connected in sequence.

[0008] One end of the light source receiving plate is bent downward to form a limiting plate. The upper part of the limiting plate is provided with a first optical fiber through hole. The upper surface of the light source receiving plate is provided with a groove for placing a cover plate. The side of the groove near the first optical fiber through hole is recessed downward to form an optical fiber guide groove. One side of the optical fiber guide groove is connected to the first optical fiber through hole, and the other side wall is provided with a second optical fiber through hole. The center of the part of the groove near the optical fiber guide groove protrudes upward to form a rectangular boss. The boss is provided with a third optical fiber through hole. The upper surface of the boss is flush with the upper surface of the light source receiving plate. The third optical fiber through hole is connected to the second optical fiber through hole. The first optical fiber passes through the first optical fiber through hole, the second optical fiber through hole and the third optical fiber through hole in sequence and protrudes from the third optical fiber through hole.

[0009] The upper surface of the light source receiving plate near the limiting plate is provided with an optical fiber fixing hole, which is connected to the first optical fiber through hole.

[0010] The first fiber optic connector female, the filter, and the second fiber optic connector female are all mounted on the filter mounting bracket.

[0011] The angle between the line connecting the second and third fiber optic vias and the bottom surface of the fiber optic guide groove is 135°.

[0012] The bottom surface of the groove has a cover plate fixing hole on the side away from the first optical fiber through hole.

[0013] The bottom surface of the cover plate is provided with a protrusion that matches the optical fiber guide groove, and the surface of the protrusion is provided with an optical fiber fixing groove. One end of the cover plate is provided with a cover plate through hole that matches the cover plate fixing hole.

[0014] The cover plate is made of a transparent material.

[0015] The filter is a narrow bandpass filter.

[0016] Among them, the first fiber optic connector, the second fiber optic connector and the third fiber optic connector are all SMA905 connectors, and the first fiber optic connector female socket and the second fiber optic connector female socket are both SMA905 connector sockets.

[0017] The working method of the fully automated immunochromatographic analyzer light source reading device of the present invention is as follows: In use, first select a suitable filter (selected according to the calibration wavelength of the fully automated immunochromatographic analyzer to be tested; the filter is a narrow-band filter, whose center wavelength matches the calibration wavelength of the equipment, and whose half-bandwidth is 10nm) and place it in the filter holder. Fix the cover plate of the filter holder. Insert the light source receiving plate into the reagent card slot of the fully automated immunochromatographic analyzer, ensuring that the limiting plate is in place. Then, insert the first fiber optic connector into the first fiber optic connector socket and tighten it. Next, insert the second fiber optic connector into the second fiber optic connector socket and tighten it. Insert the third fiber optic connector into the SMA905 connector socket of the light source reading device (such as a spectrometer). First, set the appropriate parameters on the spectrometer. Then, operate the fully automated immunochromatographic analyzer to make its detection module light source emit light. The spectrometer reads the photometric intensity in real time. Based on the read data, record the parameters of the light source (wavelength peak, half-bandwidth, center wavelength).

[0018] Compared with the prior art, the outstanding effect of the present invention is as follows:

[0019] (1) The fully automated immunochromatographic analyzer light source reading device of the present invention can guide the light source of the fully automated immunochromatographic analyzer outside the instrument through optical fiber and generate a light source that meets the requirements through a narrow bandpass filter. The entire detection process does not require disassembling and removing the light source of the fully automated immunochromatographic analyzer. It is only necessary to place the light source receiving plate (which matches the shape of the reagent card of the fully automated immunochromatographic analyzer) in the reagent card slot, and the light source can be guided out through the normal operation of the fully automated immunochromatographic analyzer. Moreover, this device solves the problem of noise during the light source reading process and highlights the digital characteristics of the required light source range.

[0020] (2) The fully automated immunochromatographic analyzer light source reading device of the present invention has the first fiber optic connector female through hole and the second fiber optic connector female through hole on a straight line, which ensures that the light can enter the filter vertically and exit the filter vertically, so as to minimize the loss of light intensity of the light source.

[0021] (3) In this invention, the line connecting the second fiber via and the third fiber via forms an angle of 135° with the bottom surface of the fiber guide groove. This angle is consistent with the input direction of the light source of the fully automated immunochromatographic analyzer, and this angle minimizes the loss of light intensity of the light source.

[0022] (4) In this invention, a limiting plate is provided on the light source receiving plate to ensure the consistency and repeatability of the detection results. The fiber guide groove can fix the first fiber, ensuring the consistency and repeatability of the detection results. The side-by-side design of multiple fiber through holes makes it possible to detect digital indicators at different positions of the light source.

[0023] (5) The device of the present invention fills the gap in the current lack of detection of the accuracy of the light source of the fully automated immunochromatographic analyzer. The device is easy to use and does not require disassembling the instrument. The light source of the instrument is exported through a specially designed optical path system, and the specific light source parameters can be obtained through a spectrometer. It is of great significance for the inspection and maintenance of the light source of the fully automated immunochromatographic analyzer.

[0024] The fully automated immunochromatographic analyzer light source reading device and its working method described in this invention will be further explained below with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the light source reading device of a fully automated immunochromatographic analyzer (taking a single optical fiber as an example).

[0026] Figure 2 This is a schematic diagram of the light source receiving plate.

[0027] Figure 3 This is a schematic diagram of the cover plate.

[0028] Figure 4 This is a schematic diagram of the connection between the light source receiving board and the optical fiber (taking 5 optical fibers as an example).

[0029] Figure 5 This is a cross-sectional view of the connection between the light source receiving board and the optical fiber.

[0030] Figure 6 This is a schematic diagram of the optical path when the light source reading device of a fully automated immunochromatographic analyzer is in use.

[0031] Figures 7-9 The light source detection results of three different fully automated immunochromatographic analyzers were obtained using a fully automated immunochromatographic analyzer light source reading device.

[0032] Among them, 101-light source receiving plate, 102-cover plate, 103-first optical fiber, 104-first optical fiber connector, 105-first optical fiber connector female, 106-filter, 107-filter fixing frame, 108-second optical fiber, 109-second optical fiber connector female, 110-second optical fiber connector, 111-third optical fiber connector.

[0033] 1-First fiber optic via, 2-Fiber optic fixing hole, 3-Limiting plate, 4-Fiber optic guide groove, 5-Second fiber optic via, 6-Third fiber optic via, 7-Cover plate fixing hole, 8-Fiber optic fixing groove, 9-Cover plate via. Detailed Implementation

[0034] like Figure 1-3 , Figure 5-6 As shown, a fully automated immunochromatographic analyzer light source reading device includes a light source receiving board 101, a first optical fiber 103, a first optical fiber connector 104, a first optical fiber connector socket 105, a filter 106, a second optical fiber connector socket 109, a second optical fiber connector 110, a second optical fiber 108, and a third optical fiber connector 111 connected in sequence.

[0035] One end of the light source receiving plate 101 is bent downward to form a limiting plate 3. The upper part of the limiting plate 3 is provided with a first optical fiber through hole 1. The upper surface of the light source receiving plate 101 is provided with a groove for placing a cover plate 102. The side of the groove near the first optical fiber through hole 1 is recessed downward to form an optical fiber guide groove 4. One side of the optical fiber guide groove 4 is connected to the first optical fiber through hole 1, and the other side wall is provided with a second optical fiber through hole 5. The center of the part of the groove near the optical fiber guide groove 4 is raised upward to form a rectangular boss. The boss is provided with a third optical fiber through hole 6. The upper surface of the boss is flush with the upper surface of the light source receiving plate 101. The third optical fiber through hole 6 is connected to the second optical fiber through hole 5. The first optical fiber 103 passes through the first optical fiber through hole 1, the second optical fiber through hole 5 and the third optical fiber through hole 6 in sequence and is exposed from the third optical fiber through hole 6 for optical fiber receiving of light source.

[0036] After the first optical fiber 103 enters the light source receiving plate 101, it enters the optical fiber guide groove 4. The optical fiber guide groove 4 reduces the bending degree of the optical fiber and increases the light transmittance.

[0037] The limiting plate 3 can restrict the position of the light source receiving plate 101 within the fully automated immunochromatographic analyzer, ensuring the consistency of the detection data.

[0038] The upper surface of the light source receiving plate 101 near the limiting plate 3 is provided with an optical fiber fixing hole 2, which communicates with the first optical fiber through-hole 1. There are five of each: the first optical fiber through-hole 1, the optical fiber fixing hole 2, the second optical fiber through-hole 5, and the third optical fiber through-hole 6. The five holes of the optical fiber fixing hole 2 are arranged in two rows, with three holes in one row and two holes in the other. The five holes of the first optical fiber through-hole 1, the second optical fiber through-hole 5, and the third optical fiber through-hole 6 are all arranged in a straight line and evenly distributed. The first optical fiber 103 can be fixed to the light source receiving plate 101 through the optical fiber fixing hole 2, preventing it from moving.

[0039] The first fiber optic connector 105, the filter 106, and the second fiber optic connector 109 are all mounted on the filter holder 107. The filter holder 107 can vertically fix the filter to the middle of the two fiber optic segments, so that the two light sources are tightly fused.

[0040] The angle between the line connecting the second fiber optic via 5 and the third fiber optic via 6 and the bottom surface of the fiber optic guide groove 4 is 135°.

[0041] A cover plate fixing hole 7 is provided on the side of the bottom surface of the groove away from the first optical fiber through hole 1.

[0042] The number of first optical fibers can be 1 to 5 (e.g.) Figure 1 , Figure 4 As shown in the figure, when the number is greater than one, all optical fibers are placed side by side on a plane, which can be used to obtain the spectral characteristics of light sources at different locations or at different locations of the same light source for comparison.

[0043] The bottom surface of the cover plate 102 is provided with a protrusion that matches the optical fiber guide groove 4. The surface of the protrusion is provided with an optical fiber fixing groove 8 for fixing the optical fiber. One end of the cover plate 102 is provided with a cover plate through hole 9 that matches the cover plate fixing hole 7. There are 5 optical fiber fixing grooves 8, which can fix 5 optical fibers into the groove at the same time, ensuring the consistency of light source detection. The middle part of the part of the cover plate 102 near the protrusion is a rectangular through hole, which allows the rectangular boss in the light source receiving plate 101 to be exposed.

[0044] The cover plate 102 is made of transparent material. The filter 106 is a narrow bandpass filter (which can be customized according to the characteristics of the light source). The first fiber optic connector 104, the second fiber optic connector 110, and the third fiber optic connector 111 are all SMA905 connectors, and the first fiber optic connector female socket 105 and the second fiber optic connector female socket 109 are both SMA905 connector sockets.

[0045] Users can customize a second optical fiber 108 to meet their requirements. To ensure tight beam fusion, the diameter of the second optical fiber 108 can differ from that of the first optical fiber 103.

[0046] The working method of the light source reading device of the fully automated immunochromatographic analyzer is as follows: When using it, first select a suitable filter 106 (selected according to the nominal wavelength of the fully automated immunochromatographic analyzer to be tested; for example, if the nominal wavelength of the equipment is 525nm, then a narrow-band filter with a nominal center wavelength of 525nm and a half-bandwidth of 10nm should be selected) and place it in the filter holder 107. Fix the cover plate of the filter holder, insert the light source receiving plate 101 into the reagent card slot of the fully automated immunochromatographic analyzer, and ensure that the limiting plate 3 is in place. Then, insert the first fiber optic connector 104 into the first fiber optic connector female socket 105 and tighten it. Next, insert the second fiber optic connector 110 into the second fiber optic connector female socket 109 and tighten it. Insert the third fiber optic connector 111 into the SMA905 connector female socket of the spectrometer of the light source reading device. First, set the appropriate parameters on the spectrometer, and then operate the fully automated immunochromatographic analyzer to make its detection module light source emit light. The spectrometer reads the photometric value in real time and records the parameters of the light source (wavelength peak, half-bandwidth, and center wavelength) based on the read data.

[0047] Application Examples

[0048] The light source parameters of three different commercially available fully automated immunochromatographic analyzers were tested using the fully automated immunochromatographic analyzer light source reading device of this invention. The test results are as follows: Figure 7-9 As shown.

[0049] Figures 7-9 These are the light source parameter test results of three fully automated immunochromatographic analyzers.

[0050] pass Figure 7 As can be seen, there is a peak. The analysis is as follows: the peak height of the tested device's light source is 525.53nm, the half-bandwidth is 10.03nm, and the center wavelength is 525.03nm. The peak height and half-bandwidth meet expectations, and the center wavelength is no more than 0.5nm different from the device's nominal light source parameter of 525nm. Conclusion: Considering the device's required parameters (wavelength deviation no more than 2nm), the light source of this device meets the requirements.

[0051] pass Figure 8 As can be seen, there is a peak. The analysis is as follows: the peak height of the tested device's light source is 530.15nm, the half-bandwidth is 9.12nm, and the center wavelength is 530.42nm. The peak height and half-bandwidth are as expected, but the center wavelength is 5nm greater than the device's nominal light source parameter of 525nm. Conclusion: Considering the device's requirements (wavelength deviation no greater than 2nm), this indicates that the device's light source does not meet the requirements.

[0052] pass Figure 9As can be seen, there is no obvious peak. The analysis is as follows: the light source of the tested device did not produce the required peak in the target wavelength region; its peak height, half-bandwidth, and center wavelength are all abnormal. Conclusion: Considering the device requirements (wavelength deviation not exceeding 2nm), it indicates that the light source of this device does not meet the requirements.

[0053] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A light source reading device for a fully automated immunochromatographic analyzer, characterized in that: It includes a light source receiving board (101), a first optical fiber (103), a first optical fiber connector (104), a first optical fiber connector socket (105), a filter (106), a second optical fiber connector socket (109), a second optical fiber connector (110), a second optical fiber (108), and a third optical fiber connector (111) connected in sequence. One end of the light source receiving plate (101) is bent downward to form a limiting plate (3). The upper part of the limiting plate (3) is provided with a first optical fiber through hole (1). The upper surface of the light source receiving plate (101) is provided with a groove for placing a cover plate (102). The side of the groove near the first optical fiber through hole (1) is recessed downward to form an optical fiber guide groove (4). One side of the optical fiber guide groove (4) is connected to the first optical fiber through hole (1), and the other side of the wall is provided with a second optical fiber through hole (5). The center of the part of the groove near the optical fiber guide groove (4) is raised upward to form a rectangular boss. The boss is provided with a third optical fiber through hole (6). The upper surface of the boss is flush with the upper surface of the light source receiving plate (101). The third optical fiber through hole (6) is connected to the second optical fiber through hole (5). The first optical fiber (103) passes through the first optical fiber through hole (1), the second optical fiber through hole (5) and the third optical fiber through hole (6) in sequence and is exposed from the third optical fiber through hole (6).

2. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: The upper surface of the light source receiving plate (101) near the limiting plate (3) is provided with an optical fiber fixing hole (2), which is connected to the first optical fiber through hole (1).

3. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: The first fiber optic connector female (105), the filter (106), and the second fiber optic connector female (109) are all mounted on the filter holder (107).

4. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: The angle between the line connecting the second fiber via (5) and the third fiber via (6) and the bottom surface of the fiber guide groove (4) is 135°.

5. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: A cover plate fixing hole (7) is provided on the side of the bottom surface of the groove away from the first optical fiber through hole (1).

6. The fully automated immunochromatographic analyzer light source reading device according to claim 5, characterized in that: The bottom surface of the cover plate (102) is provided with a protrusion that is compatible with the optical fiber guide groove (4), and the surface of the protrusion is provided with an optical fiber fixing groove (8). One end of the cover plate (102) is provided with a cover plate through hole (9) that is compatible with the cover plate fixing hole (7).

7. The fully automated immunochromatographic analyzer light source reading device according to claim 6, characterized in that: The cover plate (102) is made of transparent material.

8. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: The filter (106) is a narrow bandpass filter.

9. The fully automated immunochromatographic analyzer light source reading device according to claim 1, characterized in that: The first fiber optic connector (104), the second fiber optic connector (110), and the third fiber optic connector (111) are all SMA905 connectors, and the first fiber optic connector female socket (105) and the second fiber optic connector female socket (109) are both SMA905 connector sockets.

10. The method of operating the fully automated immunochromatographic analyzer light source reading device according to any one of claims 1-9, characterized in that: In use, place the filter (106) into the filter holder (107), fix the cover plate of the filter holder, insert the light source receiving plate (101) into the reagent card slot of the fully automated immunochromatographic analyzer, and ensure that the limiting plate (3) is installed in place. Then, insert the first fiber optic connector (104) into the first fiber optic connector female socket (105) and tighten it. Next, insert the second fiber optic connector (110) into the second fiber optic connector female socket (109) and tighten it. Insert the third fiber optic connector (111) into the SMA905 connector female socket of the spectrometer, set the parameters of the spectrometer, and then operate the fully automated immunochromatographic analyzer to make its detection module light source emit light. The spectrometer reads the photometric value in real time and records the parameters of the light source, including the peak wavelength, half bandwidth and center wavelength, based on the read data.

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