A kind of selenium-rich green tea selenium protein broken wall extraction equipment

Abstract

This invention discloses a selenium-enriched green tea selenoprotein cell wall breaking extraction device, belonging to the field of selenoprotein extraction technology. It includes an extraction component, comprising a main body with an internal liquid outlet assembly. The device employs a three-stage rotor synergistic cell wall breaking structure: flexible pre-breaking, dynamic and static shearing cell wall breaking, and micro-gap fine grinding. The upper flexible teeth gently knead and disperse the tea leaves, softening the cell walls without strong impact damage. The middle spiral shearing teeth and the first spiral guide rib form a dynamic and static shearing pair, which, combined with the cavitation effect generated by the piezoelectric ceramic ultrasonic transducer, breaks down the plant cell walls through a dual action of macroscopic shearing tearing and microscopic cavitation breakdown. The lower spiral grinding protrusions and the second spiral guide rib complete the fine grinding and cell wall breaking of residual fibers, achieving graded, progressive, full-area cell wall breaking. Simultaneously, the spiral guide structure guides the material to reflux and tumble from top to bottom and bottom to top, eliminating dead zones in the flow field within the cavity and improving the cell wall breaking effect of the tea leaves.

Description

Technical Field

[0001] This invention relates to the field of selenoprotein extraction technology, and more specifically, to a cell wall breaking extraction device for selenoproteins from selenium-enriched green tea. Background Technology

[0002] Selenium is an essential trace element for the human body, participating in the synthesis and metabolism of various enzymes and playing an important role in maintaining human immune function and resisting oxidative damage. Selenium-enriched green tea, as a natural carrier of selenium, contains selenoproteins, which are the most bioavailable form of organic selenium and have broad application prospects in health foods, pharmaceutical raw materials and other fields. With the increasing market demand for high-quality selenoprotein products, the efficient extraction technology of selenoproteins from selenium-enriched green tea has become a research hotspot.

[0003] Selenin is heat-sensitive and easily oxidized, and it is encapsulated within the cell walls of tea leaves. The dense structure of the cell walls, such as cellulose and hemicellulose, makes it difficult for selenoproteins to be fully released. At the same time, factors such as excessively high temperature, oxygen contact, and mechanical impact during the extraction process can all lead to oxidative inactivation or structural damage of selenoproteins, seriously affecting product quality.

[0004] Currently, most existing extraction equipment uses a single cell wall disruption method (such as ball milling or ultrasonic disruption) to extract selenoproteins from selenium-rich green tea raw materials. However, when using the above-mentioned mechanical disruption methods to extract selenoproteins, it is necessary to rely on strong impact forces, which can easily lead to the aggregation of tea fiber and the formation of dead zones in the flow field, preventing some materials from fully contacting the disruption area. When using ultrasonic disruption to extract selenoproteins, although cavitation effects can be generated, the cavitation areas are concentrated, and the macroscopic shearing effect on coarse fibers and epidermal tissues is insufficient, resulting in incomplete cell wall disruption. This makes it difficult for selenoproteins to be fully released from the cells, limiting the extraction rate. At the same time, strong impact disruption can also cause local frictional heat generation, leading to thermal denaturation of selenoproteins.

[0005] In view of this, we propose a cell wall breaking extraction device for selenium-enriched green tea selenoprotein. Summary of the Invention

[0006] Technical problem to be solved: The purpose of this invention is to provide a device for extracting selenoprotein from selenium-enriched green tea by breaking down the cell walls, thereby solving the technical problem mentioned in the background art above.

[0007] Technical Solution: The present invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device, including an extraction component, which includes an extraction device body. The extraction device body is provided with a liquid outlet component, a first spiral guide rib for guiding flow, a second spiral guide rib for assisting grinding, an extraction component for crushing selenium-enriched green tea, and a cooling component for cooling the extraction process. The reflux component includes a filter assembly disposed inside the liquid outlet assembly for filtering the extract, and the main body of the extraction device is provided with a reflux assembly for recirculating the retained coarse fiber particles.

[0008] As an optional solution to the technical solution of this invention, the extraction device includes a main body shell, a feed inlet on the top of the main body shell, a self-sealing top cover on the top of the feed inlet, a bracket fixedly connected to the top of the main body shell, a liquid inlet on the side of the main body shell, a liquid inlet valve inside the liquid inlet, several guide plates at the bottom of the feed inlet, an extraction chamber inside the main body shell, and a grinding chamber below the extraction chamber.

[0009] As an optional solution to the technical solution of this invention, the self-sealing top cover is slidably connected to the inner wall of the support. The feed inlet and the grinding chamber are both inverted conical in shape. The liquid inlet communicates with the interior of the feed inlet. The bottom of the feed inlet communicates with the interior of the extraction chamber. The bottom of the self-sealing top cover is sealed to the top of the feed inlet. A plurality of first spiral guide ribs are fixedly connected to the inner wall of the extraction chamber. A plurality of second spiral guide ribs are fixedly connected to the inner wall of the grinding chamber. The size of the second spiral guide ribs is smaller than that of the first spiral guide ribs. The pitch of the second spiral guide ribs is smaller than that of the first spiral guide ribs. The bottom of the first spiral guide ribs and the top of the second spiral guide ribs are smoothly connected. The first spiral guide ribs and the second spiral guide ribs are spiraled in the same direction.

[0010] By adopting the above technical solution, spiral guide ribs can be set to guide the material.

[0011] As an optional solution of the technical solution in this invention document, the liquid outlet component includes a buffer cavity disposed inside the main body shell, a liquid guide cone fixedly connected to the inner wall of the buffer cavity, a plurality of leakage grooves evenly opened on the side of the liquid guide cone, a collection cavity disposed inside the main body shell, a liquid outlet disposed on the side of the main body shell, a liquid outlet valve disposed inside the liquid outlet, a sealed slag discharge port disposed at the bottom of the main body shell, and a filter cavity disposed inside the main body shell; The collecting chamber is in the shape of an inverted cone, with the tip of the liquid guiding cone facing upwards. The bottom of the grinding chamber is connected to the filtering chamber. The filtering chamber is in the shape of a regular cone, with the bottom of the filtering chamber connected to the buffer chamber. The interior of the buffer chamber is connected to the interior of the collecting chamber through a leakage groove. The liquid outlet is located on the side of the main shell away from the liquid inlet. The liquid outlet is connected to the interior of the collecting chamber. The horizontal position of the liquid outlet is above the horizontal position of the sealed slag discharge port.

[0012] As an optional solution to the technical solution of this invention, the extraction component includes a motor fixedly connected to the top of the support, an upper rotor rotatably connected to the bottom of the motor, a number of flexible teeth fixedly connected to the side of the upper rotor, a middle rotor fixedly connected to the bottom of the upper rotor, a number of helical shearing teeth evenly distributed on the side of the middle rotor, a lower rotor fixedly connected to the bottom of the middle rotor, a number of helical grinding protrusions fixedly connected to the surface of the lower rotor, and a piezoelectric ceramic ultrasonic transducer disposed inside the middle rotor.

[0013] As an optional embodiment of the technical solution in this invention document, the upper rotor extends through the top of the support into the interior of the extraction chamber, the top of the middle rotor is chamfered, the size of the middle rotor is larger than that of the upper rotor, both the upper rotor and the middle rotor are located inside the extraction chamber, the pitch of the spiral shearing teeth is greater than the pitch of the first spiral guide rib, the spiral direction of the spiral shearing teeth is opposite to the spiral direction of the first spiral guide rib, the lower rotor has an inverted conical shape, the lower rotor is located inside the grinding chamber, the size of the spiral grinding ridge is the same as that of the second spiral guide rib, the spiral direction of the spiral grinding ridge is the same as that of the spiral shearing teeth, the distance between the flexible teeth and the first spiral guide rib is greater than the distance between the spiral shearing teeth and the first spiral guide rib, and the distance between the spiral shearing teeth and the first spiral guide rib is greater than the distance between the spiral grinding ridge and the second spiral guide rib.

[0014] By adopting the above technical solution and setting up a multi-stage cell wall breaking structure, selenoproteins in the raw materials can be fully extracted.

[0015] As an optional solution of the technical solution in this invention document, the cooling assembly includes a coolant inlet and a coolant outlet disposed on the side of the main body shell, a flow-dividing ring and a flow-combining ring are provided inside the main body shell, and a plurality of cooling chambers are uniformly provided inside the main body shell; The coolant inlet communicates with the interior of the diverter ring, the coolant outlet communicates with the interior of the manifold ring, the top of the diverter ring communicates with the bottom of the cooling chamber, the bottom of the manifold ring communicates with the top of the cooling chamber, the extraction chamber is located among several cooling chambers, the diverter ring is located outside the filtration chamber, the manifold ring is located outside the feed inlet, the coolant inlet is located directly above the outlet, and the coolant outlet is located directly above the coolant inlet.

[0016] By adopting the above technical solution, a cooling component can be installed to cool the extraction component.

[0017] As an optional solution of the technical solution in this invention document, the filter assembly includes a rotating shaft fixedly connected to the bottom of the lower rotor, a plurality of first arc-shaped scrapers fixedly connected to the side wall of the rotating shaft, a plurality of second arc-shaped scrapers fixedly connected to the bottom of the rotating shaft, and a coarse filter screen and a fine filter screen fixedly connected to the inner wall of the buffer cavity, a plurality of first filter holes uniformly arranged on the coarse filter screen, and a plurality of second filter holes uniformly arranged on the fine filter screen.

[0018] As an optional solution of the technical solution in this invention document, the fine filter screen is located below the coarse filter screen, the size of the fine filter screen is larger than the size of the coarse filter screen, the size of the second filter hole is smaller than the size of the first filter hole, the bottom of the rotating shaft extends through the top of the coarse filter screen to the top of the fine filter screen, the coarse filter screen is conical in shape, the bottom of the first arc-shaped scraper is in contact with the top of the coarse filter screen, and the bottom of the second arc-shaped scraper is in contact with the top of the fine filter screen.

[0019] By adopting the above technical solution, the extract can be filtered by setting up a filtration component.

[0020] As an optional solution to the technical solution of this invention, the reflux assembly includes several reflux inlets opened on the side wall of the filter chamber, several reflux channels are opened inside the main body shell, and several reflux outlets are evenly opened on the side wall of the extraction chamber. The return inlet is located on the side of the coarse filter and the first arc-shaped scraper. The return inlet is connected to the return outlet through the return channel. The return outlet is located on the side of the flexible tooth. Several return channels are alternately distributed with several cooling chambers.

[0021] By adopting the above technical solution, the reflux component can be set up to return the particles that have not been fully broken to the extraction component for re-breaking and extraction.

[0022] Beneficial effects: One or more technical solutions provided in this invention have at least the following technical effects or advantages: 1. By adopting a three-stage rotor synergistic cell-breaking structure of flexible pre-breaking, dynamic and static shearing cell breaking, and micro-gap fine grinding, the upper flexible teeth gently knead and disperse the tea leaves, softening the cell walls without strong impact damage; the middle spiral shearing teeth and the first spiral guide rib form a dynamic and static shearing pair, which, together with the cavitation effect generated by the piezoelectric ceramic ultrasonic transducer, breaks the plant cell walls through the dual action of macroscopic shearing tearing and microscopic cavitation breakdown; the lower spiral grinding ridge and the second spiral guide rib complete the fine grinding and cell breaking of residual fibers, realizing graded progressive full-area cell breaking. At the same time, the spiral guide structure guides the material to flow back and roll from top to bottom and from bottom to top, eliminating dead corners in the flow field inside the cavity, improving the cell breaking effect of tea leaves, and significantly increasing the selenoprotein dissolution rate.

[0023] 2. Relying on the micro-pressure difference between the inside and outside of the cavity constructed by the rotor rotation and the spiral guide ribs, there is no need to configure an additional delivery pump. The large particles of unbroken material intercepted by the coarse filter screen can be automatically returned to the pre-breaking zone at the top of the cavity for secondary cell wall breaking treatment by the swirling thrust of the internal flow field and the liquid level pressure difference, until the particle size meets the standard and passes through the sieve. This effectively avoids the waste of tea raw materials, coarse fiber particles and selenium protein residue loss, making full use of raw materials and reducing production costs.

[0024] 3. The device is equipped with a first arc-shaped scraper and a second arc-shaped scraper, which rotate synchronously with the rotor. This continuously pushes large particles, ultrafine tea residue, and colloidal particles trapped by the screen to the side wall of the cavity. This not only prevents the filter pores of the coarse and fine screens from clogging and ensures the normal permeation and flow of the extract, but also helps coarse particles to quickly enter the reflux channel to participate in reflux and cell wall breaking. This avoids problems such as flow field disturbance and reflux failure caused by screen clogging. The equipment can operate continuously and stably for a long time, reducing the frequency of manual cleaning.

[0025] 4. The double-layer graded sieve structure, combining coarse and fine filters, progressively traps whole tea leaves, tea stems, coarse fibers, ultrafine tea residue, and plant colloidal particles, allowing only the clear extract containing selenium protein to pass through. After passing through a flow stabilization buffer chamber and a liquid guide cone to dissipate energy and slow flow, the extract enters an inverted cone-shaped collecting chamber, transforming high-speed seepage into stable laminar flow. This achieves natural stratification of the supernatant and sediment, with the clear middle layer of extract flowing out under pressure from the side wall outlet valve. This effectively avoids mixing of bottom sediment and top scum, resulting in a selenium protein extract with high clarity and few impurities. It can be directly fed into the subsequent concentration and drying process without additional purification, simplifying the process flow.

[0026] 5. An independent cooling circulation component is set up, forming a closed-loop cooling circuit through the coolant inlet, diversion ring, cooling chamber and confluence ring, which can stably control the temperature inside the extraction chamber at a low temperature environment below 40℃; at the same time, it is equipped with a gravity magnetic self-sealing top cover, which automatically seals the chamber after feeding, effectively isolating external light and oxygen. Combined with the suitable environment of the pH 5.0~7.5 neutral extraction solution, a protection system is formed from multiple dimensions of temperature, oxygen, light and pH, which completely avoids the oxidation, thermal denaturation and mechanical damage of selenoprotein, and preserves the bioactivity and effective component content of selenoprotein in selenium-rich green tea to the greatest extent.

[0027] 6. Furthermore, the equipment operates in a completely closed system from feeding, cell wall breaking, extraction, screening to final slag discharge. The feeding top cover is magnetically sealed, and the slag discharge uses a bottom-sealed slag discharge port. Waste tea residue and ultrafine particles can be discharged in a closed manner at one time, with no open volatilization and no odor leakage. This effectively avoids material dust, waste liquid and waste gas from polluting the working environment, ensuring the cleanliness of the selenium protein cell wall breaking extraction process of selenium-rich green tea, and helping to further improve the extraction quality and effect of selenium protein. Attached Figure Description

[0028] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of the overall structure of the equipment for extracting selenoprotein from selenium-enriched green tea.

[0030] Figure 2 A schematic cross-sectional view of the main body of the extraction equipment for selenium-enriched green tea selenoprotein cell wall breaking extraction.

[0031] Figure 3 Equipment for cell wall breaking and extraction of selenium protein from selenium-enriched green tea Figure 2 Enlarged structural diagram at point A in the middle.

[0032] Figure 4 Equipment for cell wall breaking and extraction of selenium protein from selenium-enriched green tea Figure 2 Enlarged structural diagram at point B.

[0033] Figure 5 This is a schematic cross-sectional view of the cooling component in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0034] Figure 6 This is a schematic diagram showing the structural relationship between the first and second spiral guide ribs in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0035] Figure 7This is a schematic diagram showing the structural relationship and coordination between the guide plate and the flexible teeth in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0036] Figure 8 This is a schematic diagram showing the structural relationship between the cooling chamber and the reflux channel in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0037] Figure 9 This is a schematic cross-sectional view of the liquid output component in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0038] Figure 10 This is a schematic diagram showing the structural relationship of the self-sealing top cover in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0039] Figure 11 A three-dimensional structural diagram of the extraction components in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0040] Figure 12 This is a three-dimensional structural diagram of the filtration component in a selenium-enriched green tea selenoprotein cell wall breaking extraction device.

[0041] Explanation of the numbered labels in the diagram: 10. Main body of the extraction equipment; 101. Main body shell; 102. Feed inlet; 103. Self-sealing top cover; 104. Support frame; 105. Liquid inlet; 106. Liquid inlet valve; 107. Guide plate; 108. Extraction chamber; 109. Grinding chamber; 11. Liquid outlet assembly; 111. Buffer chamber; 112. Liquid guide cone; 113. Leakage trough; 114. Collection chamber; 115. Liquid outlet; 116. Liquid outlet valve; 117. Sealed slag discharge port; 118. Filter chamber; 12. First spiral guide rib; 13. Second spiral guide rib; 14. Extraction assembly; 141. Motor; 142. Upper rotor; 143. Flexible teeth; 144. Middle rotor; 145. Spiral shearing teeth; 146. Lower rotor; 147. Spiral grinding protrusions; 148. Piezoelectric ceramic ultrasonic transducer; 15. Cooling assembly; 151. Coolant inlet; 152. Diverter ring; 153. Cooling chamber; 154. Combining ring; 155. Coolant outlet; 20. Filter assembly; 201. Rotating shaft; 202. First arc-shaped scraper; 203. Second arc-shaped scraper; 204. Coarse filter screen; 205. First filter hole; 206. Fine filter screen; 207. Second filter hole; 21. Reflux assembly; 211. Reflux inlet; 212. Reflux channel; 213. Reflux outlet. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0043] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0044] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or a link; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0045] Reference Figures 1 to 12 This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device, including an extraction component, which includes an extraction device body 10, an extraction device body 10 with a liquid outlet component 11 inside, an extraction device body 10 with a first spiral guide rib 12 for guiding flow inside, an extraction device body 10 with a second spiral guide rib 13 for assisting grinding inside, an extraction device body 10 with an extraction component 14 for breaking selenium-enriched green tea inside, and a cooling component 15 for cooling the extraction process inside. The reflux component includes a filter assembly 20 disposed inside the liquid outlet assembly 11 for filtering the extract, and a reflux assembly 21 disposed inside the extraction device body 10 for refluxing the retained coarse fiber particles.

[0046] Reference Figures 1 to 10This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The main body 10 of the extraction device includes a main body shell 101. The top of the main body shell 101 is provided with a feed inlet 102. The top of the feed inlet 102 is provided with a self-sealing top cover 103. The top of the main body shell 101 is fixedly connected with a bracket 104. The side of the main body shell 101 is provided with a liquid inlet 105. The inside of the liquid inlet 105 is provided with a liquid inlet valve 106. The bottom of the feed inlet 102 is provided with a plurality of guide plates 107. The inside of the main body shell 101 is provided with an extraction chamber 108. The bottom of the extraction chamber 108 is provided with a grinding chamber 109. The self-sealing cap 103 is slidably connected to the inner wall of the support 104. The feed inlet 102 and the grinding chamber 109 are both inverted conical in shape. The liquid inlet 105 communicates with the interior of the feed inlet 102. The bottom of the feed inlet 102 communicates with the interior of the extraction chamber 108. The bottom of the self-sealing cap 103 is sealed to the top of the feed inlet 102. Several first spiral guide ribs 12 are fixedly connected to the inner wall of the extraction chamber 108. Several second spiral guide ribs 13 are fixedly connected to the inner wall of the grinding chamber 109. The size of the second spiral guide ribs 13 is smaller than that of the first spiral guide ribs 12. The pitch of the second spiral guide ribs 13 is smaller than that of the first spiral guide ribs 12. The bottom of the first spiral guide ribs 12 and the top of the second spiral guide ribs 13 are smoothly connected. The first spiral guide ribs 12 and the second spiral guide ribs 13 are spiral in the same direction.

[0047] Reference Figures 2 to 9 This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The liquid outlet component 11 includes a buffer chamber 111 disposed inside the main body shell 101. A liquid guide cone 112 is fixedly connected to the inner wall of the buffer chamber 111. A plurality of liquid leakage grooves 113 are evenly opened on the side of the liquid guide cone 112. A collection chamber 114 is disposed inside the main body shell 101. A liquid outlet 115 is disposed on the side of the main body shell 101. A liquid outlet valve 116 is disposed inside the liquid outlet 115. A sealed slag discharge port 117 is disposed at the bottom of the main body shell 101. A filter chamber 118 is disposed inside the main body shell 101. The collecting chamber 114 is in the shape of an inverted cone, with the tip of the liquid guiding cone 112 facing upwards. The bottom of the grinding chamber 109 is connected to the filtering chamber 118. The filtering chamber 118 is in the shape of a regular cone, with the bottom of the filtering chamber 118 connected to the buffer chamber 111. The interior of the buffer chamber 111 is connected to the interior of the collecting chamber 114 through the leakage groove 113. The liquid outlet 115 is located on the side of the main body shell 101 away from the liquid inlet 105. The liquid outlet 115 is connected to the interior of the collecting chamber 114. The horizontal position of the liquid outlet 115 is above the horizontal position of the sealed slag discharge port 117.

[0048] Reference Figures 2 to 11This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The extraction component 14 includes a motor 141 fixedly connected to the top of the support 104, an upper rotor 142 rotatably connected to the bottom of the motor 141, a plurality of flexible teeth 143 fixedly connected to the side of the upper rotor 142, a middle rotor 144 fixedly connected to the bottom of the upper rotor 142, a plurality of spiral shearing teeth 145 evenly opened on the side of the middle rotor 144, a lower rotor 146 fixedly connected to the bottom of the middle rotor 144, a plurality of spiral grinding protrusions 147 fixedly connected to the surface of the lower rotor 146, and a piezoelectric ceramic ultrasonic transducer 148 is provided inside the middle rotor 144. The upper rotor 142 extends through the top of the support 104 into the interior of the extraction chamber 108. The top of the middle rotor 144 is chamfered. The size of the middle rotor 144 is larger than that of the upper rotor 142. Both the upper rotor 142 and the middle rotor 144 are located inside the extraction chamber 108. The pitch of the spiral shearing teeth 145 is greater than the pitch of the first spiral guide rib 12. The spiral direction of the spiral shearing teeth 145 is opposite to that of the first spiral guide rib 12. The lower rotor 146 has an inverted conical shape and is located inside the grinding chamber 109. The spiral grinding ridge 14... The dimensions of 7 are the same as those of the second spiral guide rib 13. The spiral direction of the spiral grinding protrusion 147 is the same as that of the spiral shearing tooth 145. The distance between the flexible tooth 143 and the first spiral guide rib 12 is greater than the distance between the spiral shearing tooth 145 and the first spiral guide rib 12. The distance between the spiral shearing tooth 145 and the first spiral guide rib 12 is greater than the distance between the spiral grinding protrusion 147 and the second spiral guide rib 13. The piezoelectric ceramic ultrasonic transducer 148 is sealed and connected to the inside of the central rotor 144. The spiral shearing tooth 145 is made of high-density rigid alloy material. By employing a three-stage rotor synergistic cell-breaking structure—flexible pre-breaking, dynamic and static shearing cell breaking, and micro-gap fine grinding—the upper flexible teeth 143 gently knead and disperse the tea leaves, softening the cell walls without strong impact damage. The middle spiral shearing teeth 145 and the first spiral guide rib 12 form a dynamic and static shearing pair, which, together with the cavitation effect generated by the piezoelectric ceramic ultrasonic transducer 148, breaks down the plant cell walls through a dual action of macroscopic shearing tearing and microscopic cavitation penetration. The lower spiral grinding protrusions 147 and the second spiral guide rib 13 complete the fine grinding and cell breaking of residual fibers, achieving graded and progressive full-area cell breaking. At the same time, the spiral guide structure guides the material to flow back and roll from top to bottom and from bottom to top, eliminating dead angles in the flow field within the cavity, improving the cell breaking effect of tea leaves, and significantly increasing the selenoprotein dissolution rate.

[0049] Reference Figures 2 to 8This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The cooling component 15 includes a cooling liquid inlet 151 and a cooling liquid outlet 155 disposed on the side of the main body shell 101. A diversion ring 152 and a confluence ring 154 are opened inside the main body shell 101. A plurality of cooling chambers 153 are uniformly opened inside the main body shell 101. The coolant inlet 151 communicates with the interior of the diverter ring 152, the coolant outlet 155 communicates with the interior of the manifold ring 154, the top of the diverter ring 152 communicates with the bottom of the cooling chamber 153, the bottom of the manifold ring 154 communicates with the top of the cooling chamber 153, the extraction chamber 108 is located among several cooling chambers 153, the diverter ring 152 is located outside the filter chamber 118, the manifold ring 154 is located outside the feed inlet 102, the coolant inlet 151 is located directly above the outlet 115, and the coolant outlet 155 is located directly above the coolant inlet 151.

[0050] Reference Figures 2 to 12 This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The filter assembly 20 includes a rotating shaft 201 fixedly connected to the bottom of the lower rotor 146. A plurality of first arc-shaped scrapers 202 are fixedly connected to the side wall of the rotating shaft 201, and a plurality of second arc-shaped scrapers 203 are fixedly connected to the bottom of the rotating shaft 201. A coarse filter screen 204 and a fine filter screen 206 are fixedly connected to the inner wall of the buffer chamber 111. A plurality of first filter holes 205 are uniformly arranged on the coarse filter screen 204, and a plurality of second filter holes 207 are uniformly arranged on the fine filter screen 206. The fine filter screen 206 is located below the coarse filter screen 204. The size of the fine filter screen 206 is larger than that of the coarse filter screen 204. The size of the second filter hole 207 is smaller than that of the first filter hole 205. The bottom of the rotating shaft 201 extends through the top of the coarse filter screen 204 and above the fine filter screen 206. The coarse filter screen 204 is conical in shape. The bottom of the first arc-shaped scraper 202 is in contact with the top of the coarse filter screen 204, and the bottom of the second arc-shaped scraper 203 is in contact with the top of the fine filter screen 206. Relying on the micro-pressure difference between the inside and outside of the cavity constructed by the rotor rotation and the spiral guide ribs, there is no need to configure an additional delivery pump. The large particles of unbroken material intercepted by the coarse filter 204 can be automatically returned to the pre-breaking zone at the top of the cavity for secondary cell breaking treatment, until the particle size meets the standard and passes through the sieve. This effectively avoids the waste of tea raw materials, coarse fiber particles and selenium protein residue loss, making full use of raw materials and reducing production costs. The device is equipped with a first arc-shaped scraper 202 and a second arc-shaped scraper 203, which rotate synchronously with the rotor. They continuously push large particles, ultrafine tea residue, and colloidal particles trapped by the screen to the side wall of the cavity. This not only prevents the filter pores of the coarse and fine screens from clogging and ensures the normal permeation and flow of the extract, but also helps coarse particles to quickly enter the reflux channel 212 to participate in reflux and cell wall breaking. This avoids problems such as flow field disturbance and reflux failure caused by screen clogging. The equipment can operate continuously and stably for a long time, reducing the frequency of manual cleaning.

[0051] Reference Figures 2 to 8 This invention provides a selenium-enriched green tea selenoprotein cell wall breaking extraction device. The reflux component 21 includes several reflux inlets 211 opened on the side wall of the filter chamber 118, several reflux channels 212 are opened inside the main body shell 101, and several reflux outlets 213 are evenly opened on the side wall of the extraction chamber 108. The return inlet 211 is located on the side of the coarse filter 204 and the first arc-shaped scraper 202. The return inlet 211 is connected to the return outlet 213 through the return channel 212. The return outlet 213 is located on the side of the flexible tooth 143. Several return channels 212 and several cooling chambers 153 are alternately distributed. The shape of the return channel 212 is arc-shaped.

[0052] This invention provides a device for extracting selenoproteins from selenium-enriched green tea by breaking down the cell walls. Its working principle and usage process are as follows: First, connect the coolant inlet 151 and coolant outlet 155 to an external coolant circulation pump and start the circulation pump. This allows the coolant to enter the cooling chamber 153 through the coolant inlet 151 and the diverter ring 152, and then flow back to the cooling pump through the manifold ring 154 and coolant outlet 155, thus maintaining a constant temperature for the extraction chamber 108 and stabilizing its temperature below 40°C. Subsequently, fill the device with a neutral extraction solution (pH) through the inlet 105. Between 5.0 and 7.5, the liquid level completely submerges the flexible teeth 143, i.e., the entire working area of ​​the multi-stage wall-breaking rotor, establishing a stable extraction liquid layer and liquid-sealed environment. Simultaneously, the motor 141 is started, causing the upper rotor 142, middle rotor 144, lower rotor 146, first arc-shaped scraper 202, and second arc-shaped scraper 203 to rotate in a direction matching the spiral direction of the spiral shearing teeth 145. The piezoelectric ceramic ultrasonic transducer 148 is also activated. Then, the self-sealing top cover 103 slides upward to open the feed inlet 102. Subsequently, the selenium-rich green tea raw material, after being moistened, is fed into the equipment through the top feed inlet 102. The self-sealing top cover 103 automatically seals. The top self-closing gravity self-sealing top cover 103 is then opened, and the pre-moistened selenium-rich green tea raw material is fed in. After feeding, the top cover is released, and it automatically falls down under its own weight. The magnetic lock automatically closes and seals, forming a sealed working environment that isolates external light and oxygen, preventing the oxidation and deactivation of selenium proteins. After entering the feed inlet 102, the raw material will pass through the guide plate 107 along the slope of the feed inlet 102 and first enter the upper flexible pre-crushing section. The flexible teeth 143 rotate under the drive of the upper rotor 142, gently kneading, dispersing and lightly tearing the tea leaves, breaking up the agglomerated clumps, unfolding the leaf fiber structure, and softening the cell wall cellulose. There is no strong impact or high temperature friction throughout the process, avoiding mechanical damage to the selenium protein. In addition, the centrifugal force generated by the rotor rotation will evenly throw the loosened material onto the first spiral guide rib 12 on the inner wall of the extraction chamber 108, guiding the material to enter the next cell wall breaking stage in an orderly manner along the spiral trajectory. The dispersed raw material enters the high-density rigid alloy spiral shear teeth 145 and the first spiral guide rib 12 fixed to the inner wall of the cavity to form a "moving rotor + After the static guide ribs are connected to the dynamic shear pair, the opposite rotation directions and the oblique opposing slopes of the two will form a cross wedge-shaped shear cavity in the annular gap, which will strongly shear, tear and squeeze the coarse fibers and epidermal tissue of tea leaves, macroscopically tearing the fiber structure. Meanwhile, the piezoelectric ceramic ultrasonic transducer 148 sealed and integrated inside the central rotor 144 works synchronously, generating high-frequency vibrations and transmitting them to the spiral shear teeth 145 and the surrounding extract, forming dense ultrasonic cavitation bubbles. The micro-jet and shock wave generated by the collapse of the bubbles will penetrate the cell wall and form tiny pores on a microscopic scale, fully releasing the selenoprotein tightly wrapped in the cell into the extract. The first spiral guide rib 12 decomposes the horizontal circumferential flow into a downward axial thrust, forcing the material to flow back and roll along the spiral trajectory from top to bottom and then from bottom to top, repeatedly shuttling between the spiral shear teeth 145 and the first spiral guide rib 12, achieving uniform cell wall breaking throughout the entire area without dead zones in the flow field. The material after intermediate processing will continue to flow down to the next micro-gap fine grinding and cell breaking section. The spiral grinding protrusions 147 on the conical lower rotor 146 cooperate with the second spiral guide ribs 13 on the inner wall of the grinding chamber 109 to squeeze, knead and micro-shear the remaining intact cells and coarse fiber fragments. This completely breaks the cell wall binding, achieves three-stage cell breaking and extraction, and guides the material to diffuse evenly to the lower double-layer grading screen, avoiding the local sedimentation and accumulation of large particles, creating conditions for subsequent screening and reflux. When the material after cell wall breaking passes through the coarse filter 204, the large particles that do not meet the standards, whole tea leaves, long tea stems, and unbroken coarse fibers will be trapped above the coarse filter 204, while the remaining components will flow through the first filter hole 205 to the fine filter 206. The fine filter 206 will intercept ultrafine tea residue, plant colloids and suspended particles, allowing only the clear extract containing selenium protein to penetrate downwards. The rotor rotation and the spiral guide ribs together create a slight pressure difference between the upper and lower parts of the cavity. Combined with the backflow suction generated above the rotor at the backflow outlet 213, the large particles intercepted by the coarse screen are carried to the backflow inlet 211 on the side wall. Relying on the stable liquid level pressure difference and swirling thrust in the cavity, the coarse particles return from bottom to top along the arc-shaped backflow channel 212 to the pre-crushing zone at the top of the cavity, and re-enter the three-stage cell-breaking process to achieve forced circulation and secondary cell-breaking until the particle size meets the standard and passes through the coarse screen. No additional conveying pump is required throughout the process. It is driven purely by the internal flow field structure of the equipment, avoiding raw material waste and selenium protein loss. The second arc-shaped scraper 203 set on the coarse filter screen 204 and the fine filter screen 206 can push the intercepted particles towards the side wall by rotating, preventing the particles from clogging the first filter hole 205 and the second filter hole 207, which would prevent the extract from passing through the filter assembly 20 and thus indirectly affect the above-mentioned backflow. At the same time, the first arc-shaped scraper 202 pushing the coarse particles towards the side wall can also help the coarse particles to quickly participate in the backflow. In a low-temperature, mildly disturbed, and closed anaerobic environment, the released selenoproteins are fully dissolved in a neutral extraction solution with a pH of 5.0 to 7.5. The clear extract flowing downwards also carries away the heat generated by the rotor breaking and ultrasonic operation. Combined with the cooling component 15, it can maintain a constant temperature throughout the entire process, further preventing thermal denaturation and oxidative inactivation of the selenoproteins. The clear selenium protein extract that passes through the double-layer sieve then enters the lower flow stabilizing buffer chamber 111. After being slowly guided by the liquid guide cone 112, it falls into the inverted cone-shaped collecting chamber 114, eliminating the remaining kinetic energy of the extract that has passed through the double-layer sieve and converting the high-speed seepage into a slow laminar flow. This allows the extract to smoothly separate into layers within the inverted cone collecting chamber 114: the clear supernatant floats to the top, while the ultrafine tea residue and colloidal particles sink, preventing the liquid from rushing directly to the bottom of the cone and stirring up sediment, which would mix into the effluent and cause the extract to become turbid and its purity to drop significantly. Subsequently, the middle layer of clear supernatant flows out under pressure from the outlet valve 116 on the side wall of the collecting chamber 114 and is directly transported to the subsequent concentration and drying processes, preventing the top floating residue from mixing with the bottom sediment and ensuring the purity of the extract. Finally, after the set cyclic extraction time is reached, the rotor rotation and ultrasonic system operation are stopped, the feed inlet 102 is closed, the fine filter 206 is removed and cleaned, and the sealed slag discharge port 117 at the bottom of the collecting chamber 114 is opened to discharge all the waste tea residue and ultrafine particles that have settled at the bottom of the cone in a sealed manner. The entire process is open, odorless, and pollution-free, ensuring the cleanliness of the selenium protein cell wall breaking extraction process of selenium-rich green tea, which helps to further improve the extraction quality and effect of selenium protein.

[0053] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for extracting selenoprotein from selenium-enriched green tea by breaking down cell walls, characterized in that: include The extraction component includes an extraction device body (10), an extraction device body (10) having a liquid outlet assembly (11) inside, a first spiral guide rib (12) for guiding flow inside, a second spiral guide rib (13) for assisting grinding inside, an extraction assembly (14) for crushing selenium-rich green tea inside, and a cooling assembly (15) for cooling the extraction process inside. The reflux component includes a filter assembly (20) disposed inside the liquid outlet assembly (11) for filtering the extract, and the main body (10) of the extraction device is provided with a reflux assembly (21) for refluxing the retained coarse fiber particles.

2. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 1, characterized in that: The extraction device body (10) includes a main shell (101), a feed inlet (102) is provided on the top of the main shell (101), a self-sealing top cover (103) is provided on the top of the feed inlet (102), a bracket (104) is fixedly connected to the top of the main shell (101), a liquid inlet (105) is provided on the side of the main shell (101), a liquid inlet valve (106) is provided inside the liquid inlet (105), a plurality of guide plates (107) are provided at the bottom of the feed inlet (102), an extraction chamber (108) is provided inside the main shell (101), and a grinding chamber (109) is provided below the extraction chamber (108).

3. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 2, characterized in that: The self-sealing cap (103) is slidably connected to the inner wall of the support (104). The feed inlet (102) and the grinding chamber (109) are both inverted conical in shape. The liquid inlet (105) communicates with the interior of the feed inlet (102). The bottom of the feed inlet (102) communicates with the interior of the extraction chamber (108). The bottom of the self-sealing cap (103) is sealed to the top of the feed inlet (102). Several first spiral guides are fixedly connected to the inner wall of the extraction chamber (108). The inner wall of the grinding chamber (109) is fixedly connected with several second spiral guide ribs (13). The size of the second spiral guide ribs (13) is smaller than that of the first spiral guide ribs (12). The pitch of the second spiral guide ribs (13) is smaller than that of the first spiral guide ribs (12). The bottom of the first spiral guide ribs (12) and the top of the second spiral guide ribs (13) are smoothly connected. The first spiral guide ribs (12) and the second spiral guide ribs (13) are spiraled in the same direction.

4. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 2, characterized in that: The liquid outlet assembly (11) includes a buffer chamber (111) disposed inside the main body shell (101), a liquid guide cone (112) is fixedly connected to the inner wall of the buffer chamber (111), a plurality of leakage grooves (113) are evenly opened on the side of the liquid guide cone (112), a collection chamber (114) is disposed inside the main body shell (101), a liquid outlet (115) is disposed on the side of the main body shell (101), a liquid outlet valve (116) is disposed inside the liquid outlet (115), a sealed slag discharge port (117) is disposed at the bottom of the main body shell (101), and a filter chamber (118) is disposed inside the main body shell (101). The collecting chamber (114) is in the shape of an inverted cone. The top of the liquid guiding cone (112) faces upward. The bottom of the grinding chamber (109) is connected to the filter chamber (118). The filter chamber (118) is in the shape of a regular cone. The bottom of the filter chamber (118) is connected to the buffer chamber (111). The interior of the buffer chamber (111) is connected to the interior of the collecting chamber (114) through the leakage groove (113). The liquid outlet (115) is located on the side of the main shell (101) away from the liquid inlet (105). The liquid outlet (115) is connected to the interior of the collecting chamber (114). The horizontal position of the liquid outlet (115) is above the horizontal position of the sealed slag discharge port (117).

5. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 4, characterized in that: The extraction component (14) includes a motor (141) fixedly connected to the top of the support (104), an upper rotor (142) rotatably connected to the bottom of the motor (141), a number of flexible teeth (143) fixedly connected to the side of the upper rotor (142), a middle rotor (144) fixedly connected to the bottom of the upper rotor (142), a number of spiral shearing teeth (145) evenly opened on the side of the middle rotor (144), a lower rotor (146) fixedly connected to the bottom of the middle rotor (144), a number of spiral grinding ridges (147) fixedly connected to the surface of the lower rotor (146), and a piezoelectric ceramic ultrasonic transducer (148) is provided inside the middle rotor (144).

6. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 5, characterized in that: The upper rotor (142) extends through the top of the support (104) into the interior of the extraction chamber (108). The top of the middle rotor (144) is chamfered. The size of the middle rotor (144) is larger than that of the upper rotor (142). Both the upper rotor (142) and the middle rotor (144) are located inside the extraction chamber (108). The pitch of the spiral shearing teeth (145) is greater than the pitch of the first spiral guide rib (12). The spiral direction of the spiral shearing teeth (145) is opposite to the spiral direction of the first spiral guide rib (12). The lower rotor (146) has an inverted conical shape. The rotor (146) is located inside the grinding chamber (109). The size of the spiral grinding protrusion (147) is the same as the size of the second spiral guide rib (13). The spiral direction of the spiral grinding protrusion (147) is the same as the spiral direction of the spiral shearing tooth (145). The distance between the flexible tooth (143) and the first spiral guide rib (12) is greater than the distance between the spiral shearing tooth (145) and the first spiral guide rib (12). The distance between the spiral shearing tooth (145) and the first spiral guide rib (12) is greater than the distance between the spiral grinding protrusion (147) and the second spiral guide rib (13).

7. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 5, characterized in that: The cooling assembly (15) includes a coolant inlet (151) and a coolant outlet (155) disposed on the side of the main body shell (101). A flow divider ring (152) and a flow combiner ring (154) are provided inside the main body shell (101). A plurality of cooling chambers (153) are uniformly provided inside the main body shell (101). The coolant inlet (151) communicates with the interior of the diverter ring (152), the coolant outlet (155) communicates with the interior of the manifold ring (154), the top of the diverter ring (152) communicates with the bottom of the cooling chamber (153), the bottom of the manifold ring (154) communicates with the top of the cooling chamber (153), the extraction chamber (108) is located between several cooling chambers (153), the diverter ring (152) is located outside the filter chamber (118), the manifold ring (154) is located outside the feed inlet (102), the coolant inlet (151) is located directly above the outlet (115), and the coolant outlet (155) is located directly above the coolant inlet (151).

8. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 7, characterized in that: The filter assembly (20) includes a rotating shaft (201) fixedly connected to the bottom of the lower rotor (146). A plurality of first arc-shaped scrapers (202) are fixedly connected to the side wall of the rotating shaft (201), and a plurality of second arc-shaped scrapers (203) are fixedly connected to the bottom of the rotating shaft (201). A coarse filter screen (204) and a fine filter screen (206) are fixedly connected to the inner wall of the buffer chamber (111). A plurality of first filter holes (205) are uniformly arranged on the coarse filter screen (204), and a plurality of second filter holes (207) are uniformly arranged on the fine filter screen (206).

9. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 8, characterized in that: The fine filter (206) is located below the coarse filter (204). The size of the fine filter (206) is larger than that of the coarse filter (204). The size of the second filter hole (207) is smaller than that of the first filter hole (205). The bottom of the rotating shaft (201) extends through the top of the coarse filter (204) to the top of the fine filter (206). The coarse filter (204) is conical in shape. The bottom of the first arc-shaped scraper (202) is in contact with the top of the coarse filter (204), and the bottom of the second arc-shaped scraper (203) is in contact with the top of the fine filter (206).

10. The selenium-enriched green tea selenoprotein cell wall breaking extraction equipment according to claim 9, characterized in that: The reflux assembly (21) includes several reflux inlets (211) opened on the side wall of the filter chamber (118), several reflux channels (212) are opened inside the main body shell (101), and several reflux outlets (213) are evenly opened on the side wall of the extraction chamber (108). The return inlet (211) is located on the side of the coarse filter (204) and the first arc-shaped scraper (202). The return inlet (211) is connected to the return outlet (213) through the return channel (212). The return outlet (213) is located on the side of the flexible tooth (143). Several return channels (212) and several cooling chambers (153) are alternately distributed.

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