A rotary type super-speed programmed cooling system

Abstract

The application discloses a rotary type ultra-speed programmed cooling system, which comprises a storage device, a plurality of cooling cabins are arranged in the storage device, a rotating assembly is arranged between the plurality of cooling cabins, the rotating assembly can rotate in the storage device, and the plurality of cooling cabins can perform step-by-step programmed cooling on samples on the rotating assembly. The application has the beneficial effects that the containing cavity can be driven to rotate by the rotating cylinder, the samples in the containing cavity can be subjected to step-by-step programmed cooling by the plurality of cooling cabins, and the samples in the containing cavity can be further subjected to auxiliary cooling by the micron-level liquid nitrogen spraying mechanism; the system can switch different temperature control cavities by rotating the rotating cylinder, match a multi-stage differentiated variable-speed cooling program, and accurately realize a complete cryopreservation process of 4.0 DEG C constant temperature maintenance, gradient variable-speed cooling, low-temperature constant temperature preservation and the like, and a dynamic uniform temperature field is constructed by relying on a liquid nitrogen atomization spraying and electric refrigeration collaborative coupling mode.

Description

Technical Field

[0001] This invention relates to the field of sample storage technology, and in particular to a rotary high-speed programmed cooling system. Background Technology

[0002] Traditional biological sample cooling equipment suffers from several industry pain points, including low throughput, single cooling rate, poor temperature field uniformity, low temperature control accuracy, unstable multi-stage cooling connection, high sample ice crystal damage rate, and low recovery survival rate. To address these issues, the inventors designed a rotary high-speed programmed cooling system. Summary of the Invention

[0003] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0004] In view of the problems existing in the above or prior art, the present invention is proposed.

[0005] Therefore, the purpose of this invention is to provide a rotary high-speed programmed cooling system, which can drive the containment chamber to rotate through a rotating cylinder, and can perform stepped programmed cooling of the sample in the containment chamber through multiple cooling chambers, and can further assist in cooling the sample in the containment chamber through a micron-level liquid nitrogen spray mechanism; this system can switch between different temperature control chambers by rotating the cylinder and matching multi-stage differentiated variable speed cooling programs, and can accurately realize a complete cryopreservation process including 4.0℃ constant temperature maintenance, gradient variable speed cooling, and low temperature constant temperature preservation, and build a dynamic uniform temperature field by relying on the synergistic coupling mode of liquid nitrogen atomization spray and electric refrigeration.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a rotary high-speed programmed cooling system, which includes a storage device, a plurality of cooling chambers are arranged inside the storage device, a rotating component is arranged between the plurality of cooling chambers, the rotating component can rotate inside the storage device, and the plurality of cooling chambers can respectively perform stepped programmed cooling on the samples on the rotating component.

[0007] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the rotating component includes a rotating cylinder, and at least one receiving chamber is provided on the rotating cylinder. The receiving chamber can accommodate multiple sets of baskets, and the rotating cylinder can drive the receiving chamber to rotate and dock with multiple cooling chambers for cooling.

[0008] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the cooling chamber includes a first cooling chamber, a second cooling chamber, and a third cooling chamber; the first cooling chamber and the second cooling chamber are respectively arranged on both sides of the rotating cylinder, and the third cooling chamber is arranged at the lower end of the rotating cylinder; the receiving chamber can be rotatably docked with the first cooling chamber, the second cooling chamber, and the third cooling chamber for cooling.

[0009] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the cooling chamber further includes an upper operating chamber. The upper operating chamber is provided at the upper end of the receiving chamber, and a sealing cover is provided above the upper operating chamber. The sealing cover can be rotated on the storage device to open or seal the upper operating chamber.

[0010] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the first cooling chamber is a -20°C cooling zone, the second cooling chamber is a -40°C cooling zone, and the third cooling chamber is a -80°C cooling zone.

[0011] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, an inflatable sealing ring is provided on the periphery of the receiving chamber, and the inflatable sealing ring can be docked and sealed with multiple cooling chambers.

[0012] In a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the rotating cylinder includes a driver, a rotating cylinder, and a central shaft; the rotating cylinder is mounted on the central shaft, the central shaft is mounted on a storage device, the driver is connected to the central shaft, and the driver can drive the rotating cylinder to rotate through the central shaft.

[0013] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the storage device is further provided with a micron-level liquid nitrogen spraying mechanism, which can assist in cooling the sample on the rotating component.

[0014] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the micron-level liquid nitrogen spraying mechanism includes a liquid supply component, a shear mixing component, and spraying pipes; the liquid supply component is connected to the shear mixing component, the shear mixing component can be connected to multiple sets of spraying pipes, and the multiple sets of spraying pipes can cool the sample on the rotating component.

[0015] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the shearing and mixing component includes a secondary shearing element; the two sides of the secondary shearing element are respectively connected to the mixing element and the tertiary microporous shear; the mixing element can mix liquid nitrogen and nitrogen gas, the secondary shearing element can shear the mixed liquid nitrogen, and the tertiary microporous shear can perform secondary refinement shearing on the sheared liquid nitrogen bubbles.

[0016] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the liquid supply component includes a liquid nitrogen tank and a nitrogen gas tank, which can supply liquid nitrogen and nitrogen gas to the shear mixing component.

[0017] As a preferred embodiment of the rotary high-speed program cooling system of the present invention, the storage device is provided with an access channel, which allows the grabbing mechanism to pass through and perform access operations on the basket in the receiving chamber.

[0018] As a preferred embodiment of the rotary high-speed programmed cooling system of the present invention, the rotating component includes a rotating cylinder with three receiving chambers. Each of the three receiving chambers is provided with multiple sets of baskets, and multiple cooling chambers can be docked with any one of the receiving chambers for cooling.

[0019] The beneficial effects of this invention are as follows: This invention can drive the containing chamber to rotate by rotating the cylindrical component, and can perform stepped programmed cooling of the sample in the containing chamber through multiple cooling chambers, and can further assist in cooling the sample in the containing chamber through a micron-level liquid nitrogen spraying mechanism; This system can accurately realize the complete cryopreservation process, including 4.0℃ constant temperature maintenance, gradient variable speed cooling, and low temperature constant temperature preservation, by rotating the cylindrical component to switch between different temperature control chambers and matching multi-stage differentiated variable speed cooling programs. It relies on the synergistic coupling mode of liquid nitrogen atomization spraying and electric refrigeration to construct a dynamic and uniform temperature field. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 This is a three-dimensional schematic diagram of a rotary overspeed programmable cooling system.

[0021] Figure 2 This is a top view of a rotary overspeed programmed cooling system.

[0022] Figure 3 for Figure 2 Schematic diagram of section AA of the medium-speed vortex-type overspeed programmable cooling system.

[0023] Figure 4 Diagram showing the cooling status of a single chamber in a rotary overspeed programmed cooling system.

[0024] Figure 5 Diagram showing the cooling status of a single chamber in a rotary overspeed programmed cooling system.

[0025] Figure 6Diagram showing the cooling status of a single chamber in a rotary overspeed programmed cooling system.

[0026] Figure 7 A perspective view of another embodiment of the rotating component of the rotary overspeed programmable cooling system.

[0027] Figure 8 for Figure 7 Top view of the medium-speed vortex-type high-speed programmed cooling system.

[0028] Figure 9 for Figure 8 Schematic diagram of the BB section of the medium-speed vortex-type high-speed programmable cooling system.

[0029] Figure 10 This is a schematic diagram of multiple chambers in a rotary overspeed programmed cooling system.

[0030] Figure 11 A three-dimensional schematic diagram of the micron-level liquid nitrogen spraying mechanism of a rotary high-speed programmed cooling system.

[0031] Reference numerals: Storage device, 1; Cooling chamber, 2; Rotating assembly, 3; Rotating cylinder, 31; Receiving chamber, 32; Basket, 33; First cooling chamber, 21; Second cooling chamber, 22; Third cooling chamber, 23; Upper operating chamber, 24; Driver, 311; Rotating cylinder, 312; Central shaft, 313; Micron-level liquid nitrogen spraying mechanism, 4; Liquid supply assembly, 41; Shearing and mixing assembly, 42; Spray pipe, 43; Secondary shearing component, 422; Mixing component, 421; Tertiary microporous shearer, 423; Liquid nitrogen tank, 411; Nitrogen tank, 412; Access channel, 6; Detailed Implementation

[0032] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0033] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments. Example 1

[0035] Reference Figures 1-6 and Figure 11 This is the first embodiment of the present invention. This embodiment provides a rotary high-speed programmed cooling system, which includes a storage device 1. The storage device 1 is provided with multiple cooling chambers 2. A rotating component 3 is provided between the multiple cooling chambers 2. By rotating the rotating component 3, the sample on the rotating component 3 can be cooled in sections by using the multiple cooling chambers 2.

[0036] Specifically, it includes a storage device 1, which has multiple cooling chambers 2 inside. A rotating assembly 3 is arranged between the multiple cooling chambers 2. The rotating assembly 3 can rotate inside the storage device 1, and the multiple cooling chambers 2 can perform step-by-step programmed cooling on the samples on the rotating assembly 3 respectively.

[0037] It should be noted that the rotating component 3 can drive the sample to rotate and rotate it to the side of the corresponding cooling chamber 2. The sample can be programmed to cool down using the cooling chambers 2 with different temperatures. This avoids the sample cooling down too quickly. By rotating and docking with the cooling chambers 2 with different temperatures, a stepped cooling is achieved, thereby protecting the sample.

[0038] Furthermore, the rotating assembly 3 includes a rotating cylinder 31, which has at least one receiving chamber 32. The receiving chamber 32 can accommodate multiple sets of baskets 33. The rotating cylinder 31 can drive the receiving chamber 32 to rotate and dock with multiple cooling chambers 2 for cooling.

[0039] It should be noted that in this embodiment, only one receiving chamber 32 is provided on the rotating cylinder 31, and multiple sets of baskets 33 are provided in the receiving chamber 32. Multiple cooling chambers 2 are provided around the rotating cylinder 31, and the temperatures of the multiple cooling chambers 2 are all different. By rotating the cylinder 31, the receiving chamber 32 is driven to rotate one side of the corresponding cooling chamber 2. The cooling chamber 2 can be used to perform programmed cooling of the sample in the receiving chamber 32. By cooperating with the rotating cylinder 31 to rotate, the sample in the receiving chamber 32 can be subjected to step-by-step programmed cooling.

[0040] It should be noted that the cooling chamber 2 can use a compressor for refrigeration, or other existing refrigeration methods.

[0041] Furthermore, the cooling chamber 2 includes a first cooling chamber 21, a second cooling chamber 22, and a third cooling chamber 23; the first cooling chamber 21 and the second cooling chamber 22 are respectively provided on both sides of the rotating cylinder 31, and the third cooling chamber 23 is provided at the lower end of the rotating cylinder 31; the receiving chamber 32 can be rotatably docked with the first cooling chamber 21, the second cooling chamber 22, and the third cooling chamber 23 for cooling.

[0042] Furthermore, the cooling chamber 2 also includes an upper operating chamber 24. The upper operating chamber 24 is provided at the upper end of the receiving chamber 32, and a sealing cover 26 is provided above the upper operating chamber 24. The sealing cover 26 can be rotated on the storage device 1 to open or seal the upper operating chamber 24.

[0043] It should be noted that batch operations, storage and retrieval, etc., can be performed by rotating the sealing cover 26.

[0044] Furthermore, the first cooling chamber 21 is a -20℃ cooling zone, the second cooling chamber 22 is a -40℃ cooling zone, and the third cooling chamber 23 is a -80℃ cooling zone.

[0045] Furthermore, an inflatable sealing ring is provided around the periphery of the accommodating chamber 32, which can be docked and sealed with multiple cooling chambers 2.

[0046] It should be noted that the inflatable sealing ring can be inflated to achieve a docking seal, thereby enabling the corresponding cooling chamber 2 to dock and cool the sample in the receiving chamber 32, ensuring that the cold air will not leak out.

[0047] Furthermore, the rotating cylinder 31 includes a driver 311, a rotating cylinder 312, and a central shaft 313; the rotating cylinder 312 is mounted on the central shaft 313, the central shaft 313 is mounted on the storage device 1, the driver 311 is connected to the central shaft 313, and the driver 311 can drive the rotating cylinder 312 to rotate through the central shaft 313.

[0048] Preferably, the driver 311 can drive the central shaft 313 to rotate, and the central shaft 313 can drive the rotating cylinder 312 to rotate. The rotating cylinder 312 can drive the receiving chamber 32 to rotate and dock with the first cooling chamber 21, the second cooling chamber 22, the third cooling chamber 23 or the upper operating chamber 24.

[0049] Furthermore, the storage device 1 is also equipped with a micron-level liquid nitrogen spraying mechanism 4, which can assist in cooling the sample on the rotating component 3.

[0050] Furthermore, the micron-level liquid nitrogen spraying mechanism 4 includes a liquid supply component 41, a shearing and mixing component 42, and a spraying pipe 43; the liquid supply component 41 is connected to the shearing and mixing component 42, and the shearing and mixing component 42 can be connected to multiple sets of spraying pipes 43, which can cool the sample on the rotating component 3.

[0051] It should be noted that the micron-level liquid nitrogen spraying mechanism 4 is a nitrogen atomization spraying scheme.

[0052] It should be noted that liquid nitrogen and nitrogen gas can be supplied to the shear mixing component 42 through the liquid supply component 41. The shear mixing component 42 can shear and break up the bubbles generated by the mixed liquid nitrogen and nitrogen gas. The broken bubbles are sprayed through the spray pipe 43 to cool the sample.

[0053] It should be noted that spray pipes 43 may be installed in the first cooling chamber 21, the second cooling chamber 22, the third cooling chamber 23 and the upper operating chamber 24; nitrogen spraying can be carried out through the spray pipes 43 for cooling.

[0054] Furthermore, the shearing and mixing assembly 42 includes a secondary shearing element 422; the two sides of the secondary shearing element 422 are respectively connected to the mixing element 421 and the tertiary microporous shear 423; the mixing element 421 can mix liquid nitrogen and nitrogen gas, the secondary shearing element 422 can shear the mixed liquid nitrogen, and the tertiary microporous shear 423 can perform secondary refinement shearing on the sheared liquid nitrogen bubbles.

[0055] Preferably, the secondary shearing element 422 can mechanically shear and break the mixed liquid nitrogen bubbles into smaller bubbles, and the smaller bubbles are further sheared and broken by the tertiary microporous shearing element 423, thereby meeting the requirements of micron-level liquid nitrogen. The sample in the basket containing the chamber 32 can be cooled through the spray pipe 43.

[0056] Furthermore, the liquid supply assembly 41 includes a liquid nitrogen tank 411 and a nitrogen gas tank 412, which can supply liquid nitrogen and nitrogen gas into the shear mixing assembly 42.

[0057] Furthermore, the storage device 1 is provided with an access channel 6, through which the gripping mechanism can pass and perform access operations on the basket 33 in the receiving chamber 32.

[0058] It should be noted that the rotating cylinder 31 may also be provided with multiple receiving chambers 32, each receiving chamber 32 is provided with multiple sets of baskets 33, and the rotating cylinder 31 drives the multiple receiving chambers 32 to rotate, thereby cooperating with multiple cooling chambers 2 and micron-level liquid nitrogen spraying mechanism 4 to perform programmed cooling.

[0059] It should be noted that the storage device 1 has four chambers. The upper operating chamber 24 is an optional cooling zone, which is usually not used, but can also be connected to a micron-level generator spray line (optional) for batch programmed cooling, typically maintained at 4.0℃ for 10 minutes. The two side chambers are: the first cooling chamber 21, a -20℃ cooling zone, which is a low-temperature refrigerator containing a compressor to ensure the temperature field is at -20℃, and is connected to a micron-level generator spray line to achieve the following temperatures at a rate of 2.0℃ / min until the sample temperature reaches -5.0℃; and at a rate of 20.0℃ / min until the chamber temperature reaches -25.0℃; the second cooling chamber 22, a -40℃ cooling zone, which is a low-temperature refrigerator containing a compressor to ensure the temperature field is at -40℃, and is connected to a micron-level generator spray line to achieve the following temperatures; and the lower chamber, a -80℃ temperature field. - A nitrogen atomization spraying method is used to achieve a rate of 5.0℃ / min until the chamber temperature reaches -80.0℃; -80.0℃ is maintained for 60 minutes.

[0060] Wait for the chamber to cool down to 4.0℃, and for the cell sample bag temperature to reach 4.5℃-5.0℃, then proceed with the subsequent cryopreservation procedure; ② Maintain at 4.0℃ for 10 minutes; - in the upper operating compartment 24; ③ Continue at a rate of 2.0℃ / min until the sample temperature reaches -5.0℃; rotate the cylinder 312 to the -20℃ cooling zone. ④ At a rate of 20.0℃ / min, until the cavity temperature reaches -25.0℃; in the -20℃ cooling zone, it is cooled to -25℃ using a micron-level liquid nitrogen spraying mechanism 4 spraying technology; ⑤ Continue at a rate of 2.0℃ / min until the cavity temperature reaches -20.0℃; rotate the cylinder 312 back to the upper operating chamber 24, and the temperature returns to -20℃; ⑥ Continue at a rate of 1.0℃ / min until the cavity temperature reaches -40.0℃; then rotate cylinder 312 to the -40℃ cooling zone; ⑦ Maintain at -40.0℃ for 5 minutes; stabilize the temperature at -40℃ using atomized spray technology; ⑧ Continue at a rate of 5.0℃ / min until the cavity temperature reaches -80.0℃; then rotate cylinder 312 to the lower chamber to achieve a temperature field of -80℃; ⑨ Maintain at -80.0℃ for 60 minutes; in the lower chamber, the temperature is maintained at -80℃ through atomized spraying technology.

[0061] ⑩ End.

[0062] This device has the following characteristics; 1. With a throughput of tens of thousands, it can rapidly cool ≥10,000 2mL samples at a time, accelerating the cooling process by nearly 10 times, and achieving industrial-scale batch cryopreservation capacity.

[0063] 2. Independent compartments, wide-range temperature control, fully enclosed multi-compartment independent temperature adjustment, covering an ultra-wide temperature range of +33℃ to -196℃, and can run multiple differentiated cooling curves simultaneously, making one unit suitable for multiple scenarios.

[0064] 3. Ten-minute-level efficient cryopreservation of stem cells reduces the cooling time from the traditional 2 hours to about 12 minutes, quickly traversing the danger zone of ice crystals, and achieving a stable recovery survival rate of ≥95%.

[0065] 4. AI-powered fully automated, unattended digital twin remote control, automatic data storage throughout the process, supporting uninterrupted and stable mass production throughout the year, truly achieving "lights-out factory" level operation.

[0066] 5. Dual-mode positive pressure, frost-free and ice-free liquid nitrogen + electric refrigeration synergistic coupling, dry positive pressure environment inside the chamber, eliminating frost condensation; bubble-type atomization rotating dynamic temperature field ensures that each sample is cooled evenly without dead angles.

[0067] In summary, this invention can rotate the containing chamber by rotating the cylindrical component, and can perform stepped programmed cooling of the sample in the containing chamber through multiple cooling chambers. Furthermore, the micron-level liquid nitrogen spray mechanism can further assist in cooling the sample in the containing chamber. This system can accurately achieve a complete cryopreservation process, including maintaining a constant temperature of 4.0℃, gradient variable-speed cooling, and low-temperature constant-temperature preservation, by switching between different temperature-controlled chambers through the rotation of the cylindrical component and matching a multi-stage differentiated variable-speed cooling program. It relies on the synergistic coupling mode of liquid nitrogen atomization spray and electric refrigeration to construct a dynamic and uniform temperature field. Example 2

[0068] Reference Figures 1-2 and Figures 7-11 This is the second embodiment of the present invention. This embodiment provides a rotary high-speed programmed cooling system, which includes a storage device 1. The storage device 1 is provided with multiple cooling chambers 2. A rotating component 3 is provided between the multiple cooling chambers 2. By rotating the rotating component 3, the sample on the rotating component 3 can be cooled in sections by using the multiple cooling chambers 2.

[0069] Specifically, it includes a storage device 1, which has multiple cooling chambers 2 inside. A rotating assembly 3 is arranged between the multiple cooling chambers 2. The rotating assembly 3 can rotate inside the storage device 1, and the multiple cooling chambers 2 can perform step-by-step programmed cooling on the samples on the rotating assembly 3 respectively.

[0070] It should be noted that the rotating component 3 can drive the sample to rotate and rotate it to the side of the corresponding cooling chamber 2. The sample can be programmed to cool down using the cooling chambers 2 with different temperatures. This avoids the sample cooling down too quickly. By rotating and docking with the cooling chambers 2 with different temperatures, a stepped cooling is achieved, thereby protecting the sample.

[0071] It should be noted that the cooling chamber 2 can use a compressor for refrigeration, or other existing refrigeration methods.

[0072] It should be noted that at least two receiving chambers 32 are provided on the rotating cylinder 31, and multiple sets of baskets 33 are provided in each of the multiple receiving chambers 32. The rotating cylinder 31 can drive the multiple receiving chambers 32 to rotate and dock with the first cooling chamber 21, the second cooling chamber 22 and the third cooling chamber 23 respectively.

[0073] It should be noted that the rotating assembly 3 includes a rotating cylinder 31, on which three receiving chambers 32 are provided. Each of the three receiving chambers 32 is provided with multiple sets of baskets 33, and multiple cooling chambers 2 can be connected to any one of the receiving chambers 32 for cooling.

[0074] It should be noted that the rotating cylinder 31 drives the three accommodating chambers 32 to rotate, thereby enabling simultaneous or separate docking with the first cooling chamber 21, the second cooling chamber 22, and the third cooling chamber 23, achieving stepped cooling by docking with cooling chambers of different temperatures.

[0075] Furthermore, the cooling chamber 2 includes a first cooling chamber 21, a second cooling chamber 22, and a third cooling chamber 23; the first cooling chamber 21 and the second cooling chamber 22 are respectively provided on both sides of the rotating cylinder 31, and the third cooling chamber 23 is provided at the lower end of the rotating cylinder 31; the receiving chamber 32 can be rotatably docked with the first cooling chamber 21, the second cooling chamber 22, and the third cooling chamber 23 for cooling.

[0076] Furthermore, the cooling chamber 2 also includes an upper operating chamber 24. The upper operating chamber 24 is provided at the upper end of the receiving chamber 32, and a sealing cover 26 is provided above the upper operating chamber 24. The sealing cover 26 can be rotated on the storage device 1 to open or seal the upper operating chamber 24.

[0077] It should be noted that batch operations, storage and retrieval, etc., can be performed by rotating the sealing cover 26.

[0078] Furthermore, the first cooling chamber 21 is a -20℃ cooling zone, the second cooling chamber 22 is a -40℃ cooling zone, and the third cooling chamber 23 is a -80℃ cooling zone.

[0079] Furthermore, an inflatable sealing ring is provided around the periphery of the accommodating chamber 32, which can be docked and sealed with multiple cooling chambers 2.

[0080] It should be noted that the inflatable sealing ring can be inflated to achieve a docking seal, thereby enabling the corresponding cooling chamber 2 to dock and cool the sample in the receiving chamber 32, ensuring that the cold air will not leak out.

[0081] Furthermore, the rotating cylinder 31 includes a driver 311, a rotating cylinder 312, and a central shaft 313; the rotating cylinder 312 is mounted on the central shaft 313, the central shaft 313 is mounted on the storage device 1, the driver 311 is connected to the central shaft 313, and the driver 311 can drive the rotating cylinder 312 to rotate through the central shaft 313.

[0082] Preferably, the driver 311 can drive the central shaft 313 to rotate, and the central shaft 313 can drive the rotating cylinder 312 to rotate. The rotating cylinder 312 can drive the receiving chamber 32 to rotate and dock with the first cooling chamber 21, the second cooling chamber 22, the third cooling chamber 23 or the upper operating chamber 24.

[0083] Furthermore, the storage device 1 is also equipped with a micron-level liquid nitrogen spraying mechanism 4, which can assist in cooling the sample on the rotating component 3.

[0084] Furthermore, the micron-level liquid nitrogen spraying mechanism 4 includes a liquid supply component 41, a shearing and mixing component 42, and a spraying pipe 43; the liquid supply component 41 is connected to the shearing and mixing component 42, and the shearing and mixing component 42 can be connected to multiple sets of spraying pipes 43, which can cool the sample on the rotating component 3.

[0085] It should be noted that the micron-level liquid nitrogen spraying mechanism 4 is a nitrogen atomization spraying scheme.

[0086] It should be noted that liquid nitrogen and nitrogen gas can be supplied to the shear mixing component 42 through the liquid supply component 41. The shear mixing component 42 can shear and break up the bubbles generated by the mixed liquid nitrogen and nitrogen gas. The broken bubbles are sprayed through the spray pipe 43 to cool the sample.

[0087] It should be noted that spray pipes 43 may be installed in the first cooling chamber 21, the second cooling chamber 22, the third cooling chamber 23 and the upper operating chamber 24; nitrogen spraying can be carried out through the spray pipes 43 for cooling.

[0088] Furthermore, the shearing and mixing assembly 42 includes a secondary shearing element 422; the two sides of the secondary shearing element 422 are respectively connected to the mixing element 421 and the tertiary microporous shear 423; the mixing element 421 can mix liquid nitrogen and nitrogen gas, the secondary shearing element 422 can shear the mixed liquid nitrogen, and the tertiary microporous shear 423 can perform secondary refinement shearing on the sheared liquid nitrogen bubbles.

[0089] Preferably, the secondary shearing element 422 can mechanically shear and break the mixed liquid nitrogen bubbles into smaller bubbles, and the smaller bubbles are further sheared and broken by the tertiary microporous shearing element 423, thereby meeting the requirements of micron-level liquid nitrogen. The sample in the basket containing the chamber 32 can be cooled through the spray pipe 43.

[0090] Furthermore, the liquid supply assembly 41 includes a liquid nitrogen tank 411 and a nitrogen gas tank 412, which can supply liquid nitrogen and nitrogen gas into the shear mixing assembly 42.

[0091] Furthermore, the storage device 1 is provided with an access channel 6, through which the gripping mechanism can pass and perform access operations on the basket 33 in the receiving chamber 32.

[0092] It should be noted that the rotating cylinder 31 may also be provided with multiple receiving chambers 32, each receiving chamber 32 is provided with multiple sets of baskets 33, and the rotating cylinder 31 drives the multiple receiving chambers 32 to rotate, thereby cooperating with multiple cooling chambers 2 and micron-level liquid nitrogen spraying mechanism 4 to perform programmed cooling.

[0093] It should be noted that the storage device 1 has four chambers. The upper operating chamber 24 is an optional cooling zone, which is usually not used but can also be connected to a micron-level generator spray line for batch programmed cooling, typically maintained at 4.0℃ for 10 minutes. The two side chambers are the first cooling chamber 21, a -20℃ cooling zone, which is a low-temperature refrigerator containing a compressor to ensure the temperature field is at -20℃. Additionally, a micron-level generator spray line is connected to it to achieve the following temperature at a rate of 2.0℃ / min until... The sample temperature is lowered to -5.0℃; at a rate of 20.0℃ / min, until the chamber temperature reaches -25.0℃; the second cooling chamber 22 is a -40℃ cooling zone, a low-temperature refrigerator containing a compressor to ensure the temperature field is at -40℃, and is also connected to a micron-level generator spray pipeline to achieve the following temperature rate; the lower chamber is a -80℃ temperature field - achieved through a nitrogen atomization spray scheme at a rate of 5.0℃ / min until the chamber temperature reaches -80.0℃; -80.0℃ is maintained for 60 minutes.

[0094] Wait for the chamber to cool down to 4.0℃, and for the cell sample bag temperature to reach 4.5℃-5.0℃, then proceed with the subsequent cryopreservation procedure; ② Maintain at 4.0℃ for 10 minutes; - in the upper operating compartment 24; ③ Continue at a rate of 2.0℃ / min until the sample temperature reaches -5.0℃; rotate the cylinder 312 to the -20℃ cooling zone. ④ At a rate of 20.0℃ / min, until the cavity temperature reaches -25.0℃; in the -20℃ cooling zone, it is cooled to -25℃ using a micron-level liquid nitrogen spraying mechanism 4 spraying technology; ⑤ Continue at a rate of 2.0℃ / min until the cavity temperature reaches -20.0℃; rotate the cylinder 312 back to the upper operating chamber 24, and the temperature returns to -20℃; ⑥ Continue at a rate of 1.0℃ / min until the cavity temperature reaches -40.0℃; then rotate cylinder 312 to the -40℃ cooling zone; ⑦ Maintain at -40.0℃ for 5 minutes; stabilize the temperature at -40℃ using atomized spray technology; ⑧ Continue at a rate of 5.0℃ / min until the cavity temperature reaches -80.0℃; then rotate cylinder 312 to the lower chamber to achieve a temperature field of -80℃; ⑨ Maintain at -80.0℃ for 60 minutes; in the lower chamber, the temperature is maintained at -80℃ through atomized spraying technology.

[0095] ⑩ End.

[0096] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape and proportion of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise changed, and the nature or number or position of discrete elements may be altered or changed. Therefore, all such modifications are intended to be included within the scope of this invention. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments.

[0097] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A rotary high-speed programmed cooling system, characterized in that: The storage device (1) includes a storage device (1) with multiple cooling chambers (2) inside. A rotating assembly (3) is provided between the multiple cooling chambers (2). The rotating assembly (3) can rotate inside the storage device (1), and the multiple cooling chambers (2) can respectively perform programmed cooling on the biological samples on the rotating assembly (3). The rotating assembly (3) includes a rotating cylinder (31), and at least one receiving chamber (32) is provided on the rotating cylinder (31). The receiving chamber (32) can accommodate multiple sets of baskets (33). The rotating cylinder (31) can drive the receiving chamber (32) to rotate and dock with multiple cooling chambers (2) for cooling. The cooling chamber (2) includes a first cooling chamber (21), a second cooling chamber (22), and a third cooling chamber (23); the receiving chamber (32) is rotatably docked with the first cooling chamber (21), the second cooling chamber (22), and the third cooling chamber (23) for cooling respectively; Multiple cooling chambers (2) are cooling zones with different temperatures, and the multiple cooling chambers (2) can form a stepped programmable cooling of the sample in the containing chamber (32).

2. The rotary overspeed programmed cooling system as described in claim 1, characterized in that: The rotating cylinder (31) is provided with a first cooling chamber (21) and a second cooling chamber (22) on both sides respectively. The rotating cylinder (31) is provided with a third cooling chamber (23) at the lower end. The cooling chamber (2) also includes an upper operating chamber (24). The upper operating chamber (24) is provided at the upper end of the accommodating chamber (32). A sealing cover (26) is provided above the upper operating chamber (24). The sealing cover (26) can be rotated on the storage device (1) and can open or seal the upper operating chamber (24).

3. The rotary overspeed programmed cooling system as described in claim 1, characterized in that: The first cooling chamber (21) is a -20℃ cooling zone, the second cooling chamber (22) is a -40℃ cooling zone, and the third cooling chamber (23) is a -80℃ cooling zone.

4. The rotary overspeed programmed cooling system as described in claim 1, characterized in that: The periphery of the receiving chamber (32) is provided with an inflatable sealing ring, which can be docked and sealed with multiple cooling chambers (2).

5. The rotary overspeed programmed cooling system as described in claim 1, characterized in that: The rotating cylinder (31) includes a driver (311), a rotating cylinder (312), and a central shaft (313); the rotating cylinder (312) is mounted on the central shaft (313), the central shaft (313) is mounted on the storage device (1), the driver (311) is connected to the central shaft (313), and the driver (311) can drive the rotating cylinder (312) to rotate through the central shaft (313).

6. The rotary overspeed programmed cooling system as described in any one of claims 1 to 5, characterized in that: The storage device (1) is also equipped with a micron-level liquid nitrogen spraying mechanism (4), which can assist in cooling the sample on the rotating component (3).

7. The rotary overspeed programmed cooling system as described in claim 6, characterized in that: The micron-level liquid nitrogen spraying mechanism (4) includes a liquid supply component (41), a shear mixing component (42), and a spray pipe (43); the liquid supply component (41) is connected to the shear mixing component (42), and the shear mixing component (42) can be connected to multiple sets of spray pipes (43), and the multiple sets of spray pipes (43) can cool the sample on the rotating component (3).

8. The rotary overspeed programmed cooling system as described in claim 7, characterized in that: The shearing and mixing assembly (42) includes a secondary shearing element (422); the two sides of the secondary shearing element (422) are respectively connected to the mixing element (421) and the tertiary microporous shear (423); the mixing element (421) can mix liquid nitrogen and nitrogen gas, the secondary shearing element (422) can shear the mixed liquid nitrogen, and the tertiary microporous shear (423) can perform secondary refinement shearing on the sheared liquid nitrogen bubbles.

9. The rotary overspeed programmed cooling system as described in claim 7, characterized in that: The liquid supply assembly (41) includes a liquid nitrogen tank (411) and a nitrogen tank (412), which can supply liquid nitrogen and nitrogen gas into the shear mixing assembly (42).

10. The rotary overspeed programmed cooling system as described in any one of claims 1 to 5, characterized in that: The storage device (1) is provided with an access channel (6), which allows the grabbing mechanism to pass through and perform access operations on the basket (33) in the receiving chamber (32).

11. The rotary overspeed programmed cooling system as described in claim 10, characterized in that: The rotating assembly (3) includes a rotating cylinder (31), which has at least two receiving chambers (32). Multiple sets of baskets (33) are provided in each of the multiple receiving chambers (32), and multiple cooling chambers (2) can be connected to any one of the receiving chambers (32) for cooling.

Images