A complex probiotic dietary fiber composition for assisting in the regulation of blood glucose and a method of preparation

Abstract

The application relates to the technical field of food processing, and particularly discloses a compound probiotic dietary fiber composition for assisting in regulating blood sugar and a preparation method. The product is prepared from eight kinds of probiotic combinations, various dietary fibers, plant extracts and nutritional fortifying agents according to specific proportions. Human test results show that after taking the product, fasting blood sugar and 2-hour postprandial blood sugar are significantly reduced; moreover, the fasting blood sugar and 2-hour postprandial blood sugar of more than 80% of the test takers are reduced; clinical symptoms such as polydipsia, polyphagia, easy hunger, fatigue, polyuria and the like are effectively improved; meanwhile, defecation is more unobstructed and regular, energy and sleep quality are improved, and no adverse events occur. The application can safely and effectively assist in regulating blood sugar and improving intestinal health and overall metabolic state.

Description

Technical Field

[0001] This invention relates to the technical field of food processing, and in particular to a compound probiotic dietary fiber composition and preparation method for assisting in the regulation of blood sugar. Background Technology

[0002] In recent years, regulating blood sugar through natural ingredients such as dietary fiber, probiotics, and plant extracts has become a research hotspot. Dietary fiber can slow gastric emptying and the rate of carbohydrate absorption in the intestines, reducing postprandial blood sugar spikes; meanwhile, soluble fiber ferments in the colon to produce short-chain fatty acids, which help improve insulin sensitivity, and both have been used in adjunctive hypoglycemic products.

[0003] In the related technology, CN106858621A discloses a dietary fiber composition for regulating blood sugar and blood lipids, comprising the following components by weight percentage: 35-45% oat flour, 10-25% resistant dextrin, 10-25% wheat bran dietary fiber, 10-25% soybean dietary fiber, 5-10% crystalline fructose, and 0.1-0.2% wheat flavoring; wherein the weight ratio of oat flour and resistant dextrin to wheat bran dietary fiber and soybean dietary fiber is (1.2-1.9):1.

[0004] For people with diabetes, impaired glucose tolerance, fasting blood glucose and 2-hour postprandial blood glucose are core indicators that comprehensively reflect the pattern of blood glucose fluctuations. Although the dietary fiber composition for regulating blood glucose and blood lipids in the above-mentioned related technologies has the effect of regulating blood glucose and blood lipids, its effect of lowering fasting blood glucose and postprandial blood glucose still needs to be further improved. Summary of the Invention

[0005] In order to improve the effectiveness of dietary fiber compositions in reducing fasting blood glucose and postprandial blood glucose in people with diabetes, impaired glucose tolerance, etc., this application provides a compound probiotic dietary fiber composition and preparation method for assisting in blood glucose regulation.

[0006] In the first aspect, this application provides a compound probiotic dietary fiber composition that helps regulate blood sugar, employing the following technical solution: A compound probiotic dietary fiber composition for assisting in blood sugar regulation comprises the following raw materials in parts by weight: 10-20 parts oat bran powder, 5-15 parts resistant dextrin, 5-15 parts polydextrose, 3-10 parts oat fiber, 3-10 parts wheat fiber, 2-8 parts mulberry leaf extract, 2-6 parts L-arabinose, 2-6 parts D-tagatose, 1-5 parts konjac powder, 0.005-0.02 parts chromium chloride, 0.5-2 parts compound nutritional fortifier, 1-5 parts oat β-glucan, 1-4 parts sugarcane polyphenols, 1-4 parts kudzu root powder, 1-4 parts Japanese raisin tree powder, and 0.2-1 parts compound probiotic agent.

[0007] In one specific implementation, the compound nutritional fortifier comprises riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:(0.2-1):(98-98.8).

[0008] In one specific implementation, the compound probiotic agent includes Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28, all with equal numbers of live bacteria.

[0009] In one specific feasible implementation, the total live bacteria count of the compound probiotic agent is ≥1×10⁻⁶. 9 CFU / g.

[0010] In one specific implementation, the complex probiotic dietary fiber composition for assisting in blood sugar regulation further includes yeast powder and silicon dioxide.

[0011] Secondly, this application provides a method for preparing a compound probiotic dietary fiber composition that helps regulate blood sugar, using the following technical solution: A method for preparing a compound probiotic dietary fiber composition that helps regulate blood sugar includes the following steps: S1. Grind oat bran powder, oat fiber, wheat fiber, kudzu root powder, and Japanese raisin tree powder to D90≤75μm respectively. Grind resistant dextrin, polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, and compound nutrient fortifier to D90≤100μm respectively. Set aside. S2. Disperse konjac powder in purified water, and add polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, compound nutrient fortifier and the first portion of resistant dextrin in sequence. Stir well, filter, and obtain the adhesive liquid. S3. Dissolve the second portion of resistant dextrin in purified water to obtain a protective agent solution. Mix the compound probiotic agent with the protective agent solution and stir evenly to obtain a suspension. Spray dry the suspension to obtain primary microcapsules. The weight ratio of the first portion of resistant dextrin to the second portion of resistant dextrin is 7:3. S4. Mix oat bran powder, oat fiber, wheat fiber, kudzu root powder, Japanese raisin tree powder, and compound probiotic agent, and add them to a fluidized bed material container. Raise the bed material temperature to 35-40°C at an inlet air temperature of 50-60°C. Atomize the adhesive liquid and spray it into the material layer. After all the adhesive liquid has been sprayed, raise the inlet air temperature to 65°C and continue fluidizing and drying for 15-20 minutes. Lower the inlet air temperature to 30°C and continue fluidizing and cooling for 5 minutes. Discharge and sieve to obtain a compound probiotic dietary fiber composition with a particle size between 20-60 mesh that helps regulate blood sugar.

[0012] In one specific implementation scheme, in step S4, the atomization pressure of the adhesive liquid sprayed into the material layer is 0.2-0.3 MPa, and the spraying speed is 50-100 mL / min.

[0013] In one specific feasible embodiment, the solid content of the protective agent solution is 28-32%.

[0014] In one specific implementation, the primary microcapsules have a particle size of 50-100 μm.

[0015] In one specific feasible implementation, in step S4, the complex probiotic dietary fiber composition that helps regulate blood sugar is coated as follows: Weigh hydroxypropyl methylcellulose, polyethylene glycol 400, purified water and titanium dioxide in a weight ratio of 30:5:465:2. Heat the purified water to 70-80℃, disperse the hydroxypropyl methylcellulose in the purified water, cool to room temperature, stir until completely dissolved, add polyethylene glycol 400 and titanium dioxide, stir evenly, filter and degas to obtain the coating solution. A compound probiotic dietary fiber composition for assisting blood sugar regulation is added to a fluidized bed. Under an atomization pressure of 0.2-0.25 MPa and a spraying speed of 30-60 mL / min, the coating liquid is sprayed onto the compound probiotic dietary fiber composition for assisting blood sugar regulation. When the coating weight gain reaches 3-5%, the spraying is stopped, and fluidized drying continues for 5-10 minutes. After fluidized cooling to a material temperature ≤30℃, the material is discharged to obtain the coated compound probiotic dietary fiber composition for assisting blood sugar regulation.

[0016] In summary, this application has the following beneficial effects: 1. This application utilizes resistant dextrin, polydextrose, oat β-glucan, and konjac flour to form a highly viscous gel upon contact with water, encapsulating chyme, delaying gastric emptying, and reducing glucose diffusion rate, thereby lowering postprandial blood glucose levels. Mulberry leaf extract, L-arabinose, and D-tagatose work synergistically to inhibit α-glucosidase and sucrase, blocking the breakdown of disaccharides into monosaccharides, further reducing postprandial blood glucose. Oat fiber and wheat fiber increase chyme volume, dilute glucose concentration, and insoluble fiber accelerates intestinal peristalsis, shortens glucose contact time, making bowel movements smoother and more regular, indirectly promoting glucose excretion. Chromium chloride, kudzu root powder, and oat β-glucan work synergistically to enhance insulin sensitivity and lower fasting blood glucose. Probiotics and Hovenia dulcis powder inhibit hepatic gluconeogenesis, further reducing fasting blood glucose. Probiotics and mulberry leaf extract work synergistically to repair β-cell function; long-term use can stabilize fasting blood glucose and reduce fluctuations. Therefore, this application can improve the effectiveness of dietary fiber compositions in reducing fasting blood glucose and postprandial blood glucose in people with diabetes, impaired glucose tolerance, etc.

[0017] 2. The compound probiotic agent in this application includes eight specific strains: *Bifidobacterium animalis* subsp. *lactospirum* CP-9, *Lactobacillus johnsonii* MH-68, *Lactobacillus reuteri* GL-104, *Lactobacillus salivarius* AP-32, *Lactobacillus paracasei* K56, *Lactobacillus rhamnosus* bv77, *Lactobacillus plantarum* GLP1-LP, and *Lactobacillus plantarum* LPL28. These strains synergistically colonize the gut, produce short-chain fatty acids, improve intestinal barrier function, regulate inflammatory factors, and thus enhance insulin sensitivity. *Lactobacillus plantarum* GLP1-LP can stimulate GLP-1 secretion and control postprandial blood glucose. *Lactobacillus johnsonii* MH-68 can reduce inflammation and improve insulin sensitivity; *Bifidobacterium animalis* subsp. *lactospirum* CP-9 can produce butyrate, repair the intestinal barrier, and reduce LPS entry into the bloodstream. *Lactobacillus rhamnosus* bv77 inhibits fat synthesis and reduces intrahepatic lipids. *Lactobacillus reuteri* GL-104 promotes ABCG2 expression, and *Lactobacillus salivarius* AP-32 has antioxidant and anti-inflammatory effects. Lactobacillus paracasei K56 produces propionic acid and regulates lipid metabolism. Lactobacillus plantarum LPL28 can lower uric acid and inhibit purine absorption. The synergistic effect of these bacteria can enhance GLP-1 secretion after meals, reduce inflammation, and repair the intestinal barrier, thus regulating blood sugar in both postprandial and fasting states.

[0018] 3. The compound nutritional fortifier of this application can support energy metabolism, and chromium chloride, as a component of glucose tolerance factor, enhances insulin receptor activity.

[0019] 3. The method described in this application helps to evenly distribute water-soluble fiber, enzyme inhibitors, and probiotics, avoiding problems such as poor efficacy due to stratification and significant differences in efficacy between fasting and postprandial conditions. Formulating probiotics into probiotic microcapsules helps the strains reach the colon in a live state; otherwise, dead bacteria would be ineffective.

[0020] 4. Coating the compound probiotic dietary fiber composition that helps regulate blood sugar can prevent problems such as probiotic inactivation and decreased gelling ability of fiber after absorbing moisture, thus maintaining the stability of efficacy during the shelf life. Detailed Implementation

[0021] Unless otherwise specified, all raw materials used in this application were commercially available. Oat bran powder was purchased from Ningxia Xiangcao Biotechnology Co., Ltd. Oat fiber was purchased from Jiangsu Aofu Biotechnology Co., Ltd. Wheat fiber was purchased from Jiangsu Dongju Biotechnology Co., Ltd. Konjac powder was purchased from Hubei Haijia Biotechnology Co., Ltd. Resistant dextrin and polydextrose were purchased from Shandong Bailong Chuangyuan Biotechnology Co., Ltd. Mulberry leaf extract, 10:1 specification, was purchased from Waterles Biotechnology. L-arabinose, CAS No. 5328-37-0, was purchased from Xi'an Lavia Biotechnology Co., Ltd. D-tagatose, CAS No. 87-81-0, was purchased from Merck Group Darmstadt, Germany. Oat β-glucan, CAS No. 160872-27-5, was purchased from Hebei Hongtao Bioengineering Co., Ltd. Sugarcane polyphenols were purchased from Sichuan Huanxu Biotechnology Co., Ltd. Kudzu root powder, 10:1 specification, was purchased from Lanzhou Waterles Biotechnology Co., Ltd. Hovenia dulcis powder, 98% purity, purchased from Baoji Fangcheng Biotechnology Development Co., Ltd. Riboflavin, CAS No. 83-88-5, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Thiamine nitrate, CAS No. 532-43-4, purchased from Shanxi Zhongnuo Biotechnology Co., Ltd. Maltodextrin, CAS No. 9050-36-6, purchased from Shandong Quansheng Chemical Technology Co., Ltd. Bifidobacterium animalis subsp. lactis CP-9, preservation number CCTCC M 2014588. Lactobacillus johnsonii MH-68, preservation number CCTCC M204058. Lactobacillus reuteri GL-104, preservation number CGMCC No. 21577. Lactobacillus salivarius AP-32, preservation number ATCC 11741. Lactobacillus paracasei K56, preservation number CGMCC No. 15139. Lactobacillus rhamnosus bv77, accession number: ATCC 7469. Lactobacillus plantarum GLP1-LP, accession number: CGMCC No. 29590. Lactobacillus plantarum LPL28, accession number: CGMCC 17954.

[0022] The present application will be further described in detail below with reference to embodiments and comparative examples.

[0023] Example Example 1

[0024] This embodiment provides a compound probiotic dietary fiber composition for assisting in blood sugar regulation, comprising the following ingredients: 15 kg of oat bran powder, 10 kg of resistant dextrin, 10 kg of polydextrose, 7 kg of oat fiber, 7 kg of wheat fiber, 5 kg of mulberry leaf extract, 4 kg of L-arabinose, 4 kg of D-tagatose, 3 kg of konjac powder, 0.012 kg of chromium chloride, 1.2 kg of compound nutritional fortifier, 3 kg of oat β-glucan, 2.5 kg of sugarcane polyphenols, 2.5 kg of kudzu root powder, 2.5 kg of Japanese raisin tree powder, and 0.6 kg of compound probiotic agent.

[0025] The compound nutritional fortifier includes riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:0.6:98.4.

[0026] The compound probiotic preparation includes Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28, with equal viable counts for each species.

[0027] This embodiment also provides a method for preparing a compound probiotic dietary fiber composition that helps regulate blood sugar, comprising the following steps: S1. Grind oat bran powder, oat fiber, wheat fiber, kudzu root powder, and Japanese raisin tree powder to D90=75μm respectively. Grind resistant dextrin, polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, and compound nutrient fortifier to D90=100μm respectively. Set aside.

[0028] S2. Weigh the raw materials prepared in step S1 according to the above raw material ratio. Divide the resistant dextrin into a first resistant dextrin and a second resistant dextrin in a weight ratio of 7:3. Disperse the konjac powder in purified water, and add polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, compound nutrient fortifier and the first resistant dextrin in sequence. Stir evenly, filter, and obtain a binder with a solid content of 25%.

[0029] S3. Mix Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28 in equal proportions to obtain a total viable count ≥1×10⁻⁶. 9A compound probiotic preparation with CFU / g was prepared. The second portion of resistant dextrin was dissolved in purified water to obtain a protectant solution with a solid content of 30%. The compound probiotic preparation was weighed according to the above raw material ratio. Under sterile conditions at 4°C, the compound probiotic preparation and protectant solution were mixed and stirred until homogeneous to obtain a suspension. The suspension was then spray-dried to obtain primary microcapsules with a particle size between 50-100 μm.

[0030] S4. Mix oat bran powder, oat fiber, wheat fiber, kudzu root powder, Japanese raisin tree powder, and primary microcapsules according to the above raw material ratio, and add them to the fluidized bed material container. Raise the bed material temperature to 35-40℃ while maintaining an inlet air temperature of 50-60℃. Adjust the air volume to make the material in a stable fluidized state. Atomize the adhesive liquid and spray it into the material layer under the conditions of atomization pressure of 0.25MPa and spraying speed of 75mL / min. After all the adhesive liquid has been sprayed, raise the inlet air temperature to 65℃ and continue fluidization and drying for 18 minutes. Lower the inlet air temperature to 30℃ and continue fluidization and cooling for 5 minutes. Discharge the material, sieve it, and obtain a compound probiotic dietary fiber composition with a particle size between 20-60 mesh for assisting in blood sugar regulation.

[0031] Example 2

[0032] The only difference between this embodiment and Embodiment 1 is that the compound probiotic dietary fiber composition for assisting in blood sugar regulation includes the following ingredients: 10 kg of oat bran powder, 5 kg of resistant dextrin, 5 kg of polydextrose, 3 kg of oat fiber, 3 kg of wheat fiber, 2 kg of mulberry leaf extract, 2 kg of L-arabinose, 2 kg of D-tagatose, 1 kg of konjac powder, 0.005 kg of chromium chloride, 0.5 kg of compound nutritional fortifier, 1 kg of oat β-glucan, 1 kg of sugarcane polyphenols, 1 kg of kudzu root powder, 1 kg of Japanese raisin tree powder, and 0.2 kg of compound probiotic agent.

[0033] Example 3

[0034] The only difference between this embodiment and Embodiment 1 is that the compound probiotic dietary fiber composition for assisting in blood sugar regulation includes the following ingredients: 20 kg of oat bran powder, 15 kg of resistant dextrin, 15 kg of polydextrose, 10 kg of oat fiber, 10 kg of wheat fiber, 8 kg of mulberry leaf extract, 6 kg of L-arabinose, 6 kg of D-tagatose, 5 kg of konjac powder, 0.02 kg of chromium chloride, 2 kg of compound nutritional fortifier, 5 kg of oat β-glucan, 4 kg of sugarcane polyphenols, 4 kg of kudzu root powder, 4 kg of Japanese raisin tree powder, and 1 kg of compound probiotic agent.

[0035] Example 4

[0036] The only difference between this embodiment and Embodiment 1 is that the compound nutritional fortifier includes riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:0.2:98.8.

[0037] Example 5

[0038] The only difference between this embodiment and Embodiment 1 is that the compound nutritional fortifier includes riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:1:98.

[0039] Example 6

[0040] The only difference between this embodiment and Example 1 is that, in step S3 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the total live bacteria count of the compound probiotic agent is ≥1×10⁻⁶. 10 CFU / g.

[0041] Example 7

[0042] The only difference between this embodiment and Example 1 is that, in step S3 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the total live bacteria count of the compound probiotic agent is ≥1×10⁻⁶. 11 CFU / g.

[0043] Example 8

[0044] The only difference between this embodiment and Example 1 is that, in step S3 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the total live bacteria count of the compound probiotic agent is ≥1×10⁻⁶. 8 CFU / g.

[0045] Example 9

[0046] The only difference between this embodiment and Embodiment 1 is that, in step S4 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the adhesive liquid is atomized and sprayed into the material layer under the conditions of atomization pressure of 0.2 MPa and spraying speed of 50 mL / min.

[0047] Example 10

[0048] The only difference between this embodiment and Embodiment 1 is that, in step S4 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the adhesive liquid is atomized and sprayed into the material layer under the conditions of atomization pressure of 0.3 MPa and spraying speed of 100 mL / min.

[0049] Example 11

[0050] The only difference between this embodiment and Embodiment 1 is that, in step S3 of the preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the solid content of the protective agent solution is 28%.

[0051] Example 12

[0052] The only difference between this embodiment and Embodiment 1 is that, in step S3 of the method for preparing the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the solid content of the protective agent solution is 32%.

[0053] Example 13

[0054] The only difference between this embodiment and Embodiment 1 is that the compound probiotic dietary fiber composition for assisting in blood sugar regulation includes the following ingredients: 15 kg of oat bran powder, 10 kg of resistant dextrin, 10 kg of polydextrose, 7 kg of oat fiber, 7 kg of wheat fiber, 5 kg of mulberry leaf extract, 4 kg of L-arabinose, 4 kg of D-tagatose, 3 kg of konjac powder, 0.012 kg of chromium chloride, 1.2 kg of compound nutritional fortifier, 3 kg of oat β-glucan, 2.5 kg of sugarcane polyphenols, 2.5 kg of kudzu root powder, 2.5 kg of Japanese raisin tree powder, 0.6 kg of compound probiotic agent, 2 kg of yeast powder, and 0.6 kg of silicon dioxide.

[0055] The compound nutritional fortifier includes riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:0.6:98.4.

[0056] The compound probiotic preparation includes Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28, with equal viable counts for each species.

[0057] This embodiment also provides a method for preparing a compound probiotic dietary fiber composition that helps regulate blood sugar, comprising the following steps: S1. Grind oat bran powder, oat fiber, wheat fiber, kudzu root powder, and Japanese raisin tree powder to D90=75μm respectively. Grind resistant dextrin, polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, and compound nutrient fortifier to D90=100μm respectively. Set aside.

[0058] S2. Weigh the raw materials prepared in step S1 according to the above raw material ratio. Divide the resistant dextrin into a first resistant dextrin and a second resistant dextrin in a weight ratio of 7:3. Disperse the konjac powder in purified water, and add polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, compound nutrient fortifier and the first resistant dextrin in sequence. Stir evenly, filter, and obtain a binder with a solid content of 25%.

[0059] S3. Mix Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28 in equal proportions to obtain a total viable count ≥1×10⁻⁶. 9 A compound probiotic preparation with CFU / g was prepared. The second portion of resistant dextrin was dissolved in purified water to obtain a protectant solution with a solid content of 30%. The compound probiotic preparation was weighed according to the above raw material ratio. Under sterile conditions at 4°C, the compound probiotic preparation and protectant solution were mixed and stirred until homogeneous to obtain a suspension. The suspension was then spray-dried to obtain primary microcapsules with a particle size between 50-100 μm.

[0060] S4. According to the above raw material ratio, mix oat bran powder, oat fiber, wheat fiber, kudzu root powder, Japanese raisin tree powder, primary microcapsules, yeast powder, and silica, and add them to the fluidized bed material container. Raise the bed material temperature to 35-40℃ while maintaining an inlet air temperature of 50-60℃. Adjust the airflow to ensure the material is in a stable fluidized state. Atomize the adhesive liquid and spray it into the material layer under an atomization pressure of 0.25MPa and a spraying speed of 75mL / min. After all the adhesive liquid has been sprayed, raise the inlet air temperature to 65℃ and continue fluidizing and drying for 18 minutes. Lower the inlet air temperature to 30℃ and continue fluidizing and cooling for 5 minutes. Discharge the material, sieve it, and obtain a compound probiotic dietary fiber composition with a particle size between 20-60 mesh, which helps regulate blood sugar.

[0061] Example 14

[0062] The only difference between this embodiment and Example 1 is that, in step S4 of the method for preparing the compound probiotic dietary fiber composition for assisting in blood sugar regulation, the compound probiotic dietary fiber composition for assisting in blood sugar regulation undergoes the following coating treatment: Weigh 300 kg of hydroxypropyl methylcellulose, 50 kg of polyethylene glycol 400, 4650 kg of purified water, and 20 kg of titanium dioxide. Heat the purified water to 70-80°C, disperse the hydroxypropyl methylcellulose in the purified water, cool to room temperature, stir until completely dissolved, add polyethylene glycol 400 and titanium dioxide, stir evenly, filter, and degas to obtain the coating solution.

[0063] A compound probiotic dietary fiber composition for assisting blood sugar regulation was added to a fluidized bed. Under an atomization pressure of 0.23 MPa and a spraying speed of 45 mL / min, the coating liquid was sprayed onto the compound probiotic dietary fiber composition for assisting blood sugar regulation. When the coating weight gain reached 4%, the spraying was stopped, and fluidized drying continued for 8 minutes. After fluidized cooling to a material temperature ≤30℃, the material was discharged to obtain the coated compound probiotic dietary fiber composition for assisting blood sugar regulation.

[0064] Example 15

[0065] The only difference between this embodiment and Embodiment 14 is that the compound probiotic dietary fiber composition for assisting in blood sugar regulation is put into a fluidized bed. Under an atomization pressure of 0.2 MPa and a spraying speed of 30 mL / min, the coating liquid is sprayed onto the compound probiotic dietary fiber composition for assisting in blood sugar regulation. When the coating weight gain reaches 3%, the spraying is stopped, and fluidized drying continues for 5 minutes. After fluidized cooling to a material temperature ≤30℃, the material is discharged to obtain the coated compound probiotic dietary fiber composition for assisting in blood sugar regulation.

[0066] Example 16

[0067] The only difference between this embodiment and Embodiment 14 is that the compound probiotic dietary fiber composition for assisting in blood sugar regulation is put into a fluidized bed. Under an atomization pressure of 0.25 MPa and a spraying speed of 60 mL / min, the coating liquid is sprayed onto the compound probiotic dietary fiber composition for assisting in blood sugar regulation. When the coating weight gain reaches 5%, the spraying is stopped, and fluidized drying continues for 10 minutes. After fluidized cooling to a material temperature ≤30℃, the material is discharged to obtain the coated compound probiotic dietary fiber composition for assisting in blood sugar regulation.

[0068] Comparative Example Comparative Example 1 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain polydextrose.

[0069] Comparative Example 2 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain mulberry leaf extract.

[0070] Comparative Example 3 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain L-arabinose.

[0071] Comparative Example 4 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain D-tagatose.

[0072] Comparative Example 5 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain konjac powder.

[0073] Comparative Example 6 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain oat β-glucan.

[0074] Comparative Example 7 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain Hovenia dulcis powder.

[0075] Comparative Example 8 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain kudzu root powder.

[0076] Comparative Example 9 The only difference between this comparative example and Example 1 is that the complex probiotic dietary fiber composition for assisting in blood sugar regulation does not contain compound probiotic agents.

[0077] Performance testing The following performance tests were conducted on Examples 1-16 and Comparative Examples 1-9: According to GB 5009.88-2023 "National Food Safety Standard - Determination of Dietary Fiber in Food", the total dietary fiber content in the compound probiotic dietary fiber composition for assisting in blood sugar regulation was detected. The test results are shown in Table 1.

[0078] Layered homogeneity test: For the prepared compound probiotic dietary fiber composition for assisting in blood sugar regulation, three samples from each of the top, middle, and bottom layers were randomly selected, with each sample weighing 10±1g. Each sample was sealed and placed on a high-frequency vibration table with a vibration frequency of 30Hz and an amplitude of 2mm, and removed after vibration for 15 minutes. The total dietary fiber of the three samples was measured, and the average and standard deviation of the total dietary fiber of the three samples were calculated. Then, the homogeneity was calculated as: (standard deviation / average) × 100%. If the homogeneity was ≤5.0%, it was considered to be uniformly mixed and no layering occurred. The test results are shown in Table 1.

[0079] According to GB 2762-2022 "National Food Safety Standard - Limits of Contaminants in Food", the limits for lead and total arsenic in a compound probiotic dietary fiber composition for assisting in blood sugar regulation were determined. The test results are shown in Table 1.

[0080] Animal Trials: This animal trial employed a self-controlled pre- and post-treatment design. The trial product was a compound probiotic dietary fiber composition for assisting in blood sugar regulation, prepared in each example and comparative example. Each group included 15 mice. The intervention was administered 60 mg twice daily for 30 days. Blood glucose levels were measured before and after feeding. The data were statistically analyzed to calculate the percentage of mice whose fasting blood glucose levels decreased after 30 days of feeding compared to before the trial, and the percentage of mice whose 2-hour postprandial blood glucose levels decreased.

[0081] The percentage of mice whose fasting blood glucose levels decreased = the number of mice whose fasting blood glucose levels after 30 days of feeding were lower than their fasting blood glucose levels before the feeding experiment ÷ 15 × 100%.

[0082] The percentage of mice whose postprandial blood glucose levels decreased was calculated as follows: (Number of mice whose 2-hour postprandial blood glucose levels after 30 days of feeding were lower than their pre-feeding levels) ÷ 15 × 100%. The results are shown in Table 2.

[0083] Human Trial: This trial employed a self-controlled pre- and post-treatment design. The product used was the compound probiotic dietary fiber composition for blood sugar regulation prepared in Example 1. A total of 25 subjects were included, with a mean age of 68.24 ± 10.06 years, 64% ≥ 70 years; 36% were male and 64% were female; BMI was 24.95 ± 6.18; diabetes history distribution: 1-3 years 20%, 4-6 years 20%, 7-10 years 24%, and over 10 years 36%. The intervention was 15g twice daily for 30 days. Blood glucose levels were measured before and after administration. Data were statistically analyzed to calculate the percentage of subjects whose fasting blood glucose and 2-hour postprandial blood glucose levels decreased after 30 days compared to before the trial.

[0084] The percentage of subjects whose fasting blood glucose levels decreased = the number of subjects whose fasting blood glucose levels after 30 days of the trial were lower than their fasting blood glucose levels before the trial ÷ 25 × 100%.

[0085] The percentage of subjects whose postprandial blood glucose levels decreased was calculated as follows: (Number of subjects whose 2-hour postprandial blood glucose levels after 30 days of the trial were lower than their pre-trial 2-hour postprandial blood glucose levels) ÷ 25 × 100%. The results are shown in Table 3.

[0086] The clinical symptom scoring method (with a four-level scale of 0-3 for clinical symptoms such as excessive thirst, increased appetite, fatigue, and frequent urination, the higher the score, the more pronounced the symptoms) was used to assess changes in symptoms. The test results are shown in Table 3.

[0087] Table 1

[0088] Table 2

[0089] Table 3

[0090] Combining Example 1 and Comparative Examples 1-9 with Tables 1-3, it can be seen that compared to Example 1, the total dietary fiber content of Comparative Examples 1-6 decreased, the uniformity of Comparative Example 9 decreased, and the lead and total arsenic limits of Comparative Example 5 increased. In animal trials, the proportion of mice showing a decrease in fasting blood glucose and a decrease in postprandial blood glucose in Comparative Examples 1-9 was smaller. This indicates that using the raw material ratio and preparation method of Example 1, a compound probiotic dietary fiber composition with high total dietary fiber content, good layering uniformity, and low lead and total arsenic content can be prepared to effectively reduce fasting and postprandial blood glucose.

[0091] As can be seen from Examples 1-16 and Tables 1-3, Examples 1-16 all have a high total dietary fiber content, good layer uniformity, and low lead and total arsenic content. Furthermore, the proportion of mice showing a decrease in fasting blood glucose and a decrease in postprandial blood glucose in Examples 1-16 was relatively high. Similarly, the proportion of subjects taking the product of Example 1 showing a decrease in both fasting and postprandial blood glucose was also relatively high, with clinical symptom scores ≤1. This indicates that using the raw material ratios and preparation methods of Examples 1-16 can produce a compound probiotic dietary fiber composition with high total dietary fiber content, good layer uniformity, and low lead and total arsenic content, which can effectively reduce fasting and postprandial blood glucose in individuals with diabetes, impaired glucose tolerance, etc.

[0092] Comparison of the detection data from various embodiments shows that the total viable bacteria count using the compound probiotic agent is ≥1×10⁻⁶. 9 CFU / g, yeast powder, and silicon dioxide can further improve the effectiveness of dietary fiber compositions in reducing fasting blood glucose and postprandial blood glucose in people with diabetes, impaired glucose tolerance, etc.

[0093] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A compound probiotic dietary fiber composition for assisting in blood sugar regulation, characterized in that, The ingredients include the following parts by weight: 10-20 parts oat bran powder, 5-15 parts resistant dextrin, 5-15 parts polydextrose, 3-10 parts oat fiber, 3-10 parts wheat fiber, 2-8 parts mulberry leaf extract, 2-6 parts L-arabinose, 2-6 parts D-tagatose, 1-5 parts konjac powder, 0.005-0.02 parts chromium chloride, 0.5-2 parts compound nutritional fortifier, 1-5 parts oat β-glucan, 1-4 parts sugarcane polyphenols, 1-4 parts kudzu root powder, 1-4 parts Japanese raisin tree powder, and 0.2-1 parts compound probiotic agent.

2. The compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 1, characterized in that: The compound nutritional fortifier includes riboflavin, thiamine nitrate, and maltodextrin in a mass ratio of 1:(0.2-1):(98-98.8).

3. The compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 1, characterized in that: The compound probiotic agent includes Bifidobacterium animalis subsp. lactis CP-9, Lactobacillus johnsonii MH-68, Lactobacillus reuteri GL-104, Lactobacillus salivarius AP-32, Lactobacillus paracasei K56, Lactobacillus rhamnosus bv77, Lactobacillus plantarum GLP1-LP, and Lactobacillus plantarum LPL28, all with equal numbers of live bacteria.

4. The compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 3, characterized in that: The total viable count of the compound probiotic agent is ≥1×10⁻⁶. 9 CFU / g.

5. The compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 1, characterized in that: The compound probiotic dietary fiber composition that helps regulate blood sugar also includes yeast powder and silicon dioxide.

6. A method for preparing a compound probiotic dietary fiber composition for assisting in blood sugar regulation as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Grind oat bran powder, oat fiber, wheat fiber, kudzu root powder, and Japanese raisin tree powder to D90≤75μm respectively. Grind resistant dextrin, polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, and compound nutrient fortifier to D90≤100μm respectively. Set aside. S2. Disperse konjac powder in purified water, and add polydextrose, konjac powder, oat β-glucan, L-arabinose, D-tagatose, mulberry leaf extract, sugarcane polyphenols, chromium chloride, compound nutrient fortifier and the first portion of resistant dextrin in sequence. Stir well, filter, and obtain the adhesive liquid. S3. Dissolve the second portion of resistant dextrin in purified water to obtain a protective agent solution. Mix the compound probiotic agent with the protective agent solution and stir evenly to obtain a suspension. Spray dry the suspension to obtain primary microcapsules. The weight ratio of the first portion of resistant dextrin to the second portion of resistant dextrin is 7:

3. S4. Mix oat bran powder, oat fiber, wheat fiber, kudzu root powder, Japanese raisin tree powder, and compound probiotic agent, and add them to a fluidized bed material container. Raise the bed material temperature to 35-40°C at an inlet air temperature of 50-60°C. Atomize the adhesive liquid and spray it into the material layer. After all the adhesive liquid has been sprayed, raise the inlet air temperature to 65°C and continue fluidizing and drying for 15-20 minutes. Lower the inlet air temperature to 30°C and continue fluidizing and cooling for 5 minutes. Discharge and sieve to obtain a compound probiotic dietary fiber composition with a particle size between 20-60 mesh that helps regulate blood sugar.

7. The preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 6, characterized in that: In step S4, the atomization pressure of the adhesive liquid sprayed into the material layer is 0.2-0.3 MPa, and the spraying speed is 50-100 mL / min.

8. The preparation method of the compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 6, characterized in that: The solid content of the protective agent solution is 28-32%.

9. The method for preparing the compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 6, characterized in that: The primary microcapsules have a particle size of 50-100 μm.

10. The method for preparing the compound probiotic dietary fiber composition for assisting in blood sugar regulation according to claim 6, characterized in that, In step S4, the complex probiotic dietary fiber composition that helps regulate blood sugar is coated as follows: Weigh hydroxypropyl methylcellulose, polyethylene glycol 400, purified water and titanium dioxide in a weight ratio of 30:5:465:

2. Heat the purified water to 70-80℃, disperse the hydroxypropyl methylcellulose in the purified water, cool to room temperature, stir until completely dissolved, add polyethylene glycol 400 and titanium dioxide, stir evenly, filter and degas to obtain the coating solution. A compound probiotic dietary fiber composition for assisting blood sugar regulation is added to a fluidized bed. Under an atomization pressure of 0.2-0.25 MPa and a spraying speed of 30-60 mL / min, the coating liquid is sprayed onto the compound probiotic dietary fiber composition for assisting blood sugar regulation. When the coating weight gain reaches 3-5%, the spraying is stopped, and fluidized drying continues for 5-10 minutes. After fluidized cooling to a material temperature ≤30℃, the material is discharged to obtain the coated compound probiotic dietary fiber composition for assisting blood sugar regulation.

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