Anti-inflammatory and acne-removing composition containing curcumin diglucoside and application thereof

Abstract

The present application relates to a curcumin bisglucoside-containing anti-inflammatory acne-removing composition and application thereof, comprising the following components: curcumin bisglucoside, rosemary extract, phaeophyceae extract and willow bark extract, which can synergistically realize multiple effects of oil control, anti-inflammation, redness reduction, barrier repair, effectively avoid acne recurrence and acne mark residue, and the overall formula takes into account the efficiency and mildness, has the advantages of fast effect, good effect and mildness, and is suitable for various acne skin, even sensitive acne skin, and has a wide market application prospect.

Description

Technical Field

[0001] This invention relates to the technical field of anti-inflammatory and acne-removing cosmetics, specifically to an anti-inflammatory and acne-removing composition containing curcumin diglucoside and its application. Background Technology

[0002] Acne vulgaris is a chronic inflammatory skin disease that commonly affects adolescents and adults. Its pathogenesis mainly involves four key aspects: excessive sebum secretion, abnormal keratinization of the pilosebaceous duct, proliferation of Propionibacterium acnes, and an inflammatory cascade. Currently available acne treatment products mainly suffer from the following technical deficiencies: 1. Significant side effects from chemically synthesized ingredients. Traditional acne treatments often rely on high concentrations of salicylic acid, fruit acids, benzoyl peroxide, or retinoids. While these ingredients are fast-acting, they generally cause significant irritation, damage to the skin barrier, dryness, peeling, and photosensitivity reactions. They are especially unsuitable for long-term use by people with sensitive skin, and the relapse rate is high after discontinuation.

[0003] 2. Bottlenecks in the application of core active ingredients. Curcumin, as a recognized potent natural anti-inflammatory ingredient, has great potential in the field of acne treatment. However, free curcumin has drawbacks such as extremely poor water solubility, low transdermal absorption rate, easy decomposition upon exposure to light, and a tendency to cause yellowing of the skin, which limit its practical application in high-end cosmetics.

[0004] 3. Plant extracts have limited efficacy and lack multi-target synergy. Existing plant-based acne treatment compositions mostly use single extracts, often focusing only on one aspect of antibacterial or anti-inflammatory effects. For example, simple oil-controlling ingredients cannot resolve redness and inflammation, and simple anti-inflammatory ingredients cannot unclog pores. This single-target mechanism of action is insufficient to comprehensively block the complex pathological process of acne, resulting in incomplete acne treatment.

[0005] Therefore, there is an urgent need to develop a natural acne-removing composition and its formulation that combines highly effective anti-inflammatory, antibacterial, oil-controlling and unblocking, and barrier-repairing functions with gentleness, safety, and good stability. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide an anti-inflammatory and acne-removing composition containing curcumin diglucoside and its application.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides an anti-inflammatory and acne-removing composition containing curcumin diglucoside, comprising the following components: curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract and *Salix babylonica* bark extract; wherein the weight ratio of the curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract and *Salix babylonica* bark extract is (2-8):(1-5):(3-7):(0.5-3).

[0008] This invention uses curcumin diglucoside (CGD) to replace traditional free curcumin. This modified component, through glucosylation, significantly improves water solubility and formulation stability, solving the problems of free curcumin's easy precipitation and poor compatibility in aqueous systems. In this composition, curcumin diglucoside acts as a core anti-inflammatory agent, inhibiting NF-κB signaling pathway activation and downregulating the expression of pro-inflammatory factors such as TNF-α, thereby blocking the inflammatory cascade response from upstream.

[0009] Rosemary extract is rich in rosmarinic acid, which has significant antioxidant and mild antibacterial activity. This helps maintain skin comfort during acne treatment, reducing the incidence of adverse reactions such as dryness and redness, making it suitable for acne care of sensitive skin. At the same time, the antioxidant activity of rosmarinic acid helps maintain the stability of active ingredients in the formula.

[0010] White willow bark extract contains salicin and polyphenols, which have anti-inflammatory and mild keratin metabolism-promoting effects, helping to improve clogged pores.

[0011] The extract of *Phaeodactylum tricornutum* is rich in polyunsaturated fatty acids and extracellular polysaccharides, possessing barrier repair and soothing capabilities. It not only alleviates potential irritation during the exfoliation process but also helps maintain the stability of other active ingredients in the formula through its antioxidant activity.

[0012] Preferably, the weight ratio of curcumin diglucoside, rosemary extract, brown finger algae extract and white poplar bark extract is (3-6):(2-4):(4-6):(0.8-2).

[0013] Most preferably, the weight ratio of curcumin diglucoside, rosemary extract, brown finger algae extract and white poplar bark extract is 5:3:5:1.

[0014] In a second aspect, the present invention provides the application of the anti-inflammatory and acne-removing composition containing curcumin diglucoside described in the first aspect in the preparation of acne-removing cosmetics.

[0015] Preferably, the acne-removing cosmetic includes serum, cream, gel, mask or spray; the amount of the anti-inflammatory acne-removing composition containing curcumin diglucoside added is 0.5-3%.

[0016] Thirdly, the present invention provides an anti-inflammatory acne-removing essence, comprising the following raw materials by weight percentage: 0.5-3% of the anti-inflammatory acne-removing composition containing curcumin diglucoside in the first aspect, 3-8% of moisturizer, 0.05-0.15% of antioxidant, 0.01-0.1% of chelating agent, 0.15-0.6% of thickener, 0.01-0.1% of pH adjuster, and 0.08-0.3% of preservative, with the balance being deionized water.

[0017] Preferably, the moisturizer is at least one selected from butylene glycol, glycerin, 1,2-hexanediol, propylene glycol, 1,2-pentanediol, and sodium hyaluronate.

[0018] Preferably, the antioxidant is at least one selected from p-hydroxyacetophenone, tea polyphenols, tocopherol, tocopheryl acetate, and ferulic acid.

[0019] Preferably, the chelating agent is disodium EDTA.

[0020] Preferably, the thickener is at least one selected from acrylate / C10-30 alkanol acrylate crosspolymer, ammonium acryloyldimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, carbomer, xanthan gum, sodium polyacrylate and hydroxyethyl cellulose.

[0021] Preferably, the pH adjuster is triethanolamine and / or arginine.

[0022] Preferably, the preservative is at least one selected from phenoxyethanol, ethylhexylglycerin, octyl glycol, and 1,2-hexanediol.

[0023] Fourthly, the present invention provides a method for preparing the anti-inflammatory and acne-removing essence of the third aspect, comprising the following steps: S1. Add the chelating agent and some deionized water to the reaction vessel, stir to dissolve, then add the humectant and antioxidant, heat to 75-85℃, keep warm and stir to obtain the aqueous phase; S2. Add a thickener to the aqueous phase, stir until uniform, then cool to 40-45℃, add the anti-inflammatory and acne-removing composition, preservative and remaining deionized water, stir until uniformly dissolved to obtain the liquid. S3. Add pH adjuster to the liquid, stir, filter and discharge to obtain the anti-inflammatory and acne-removing essence.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows: The main components of the anti-inflammatory and acne-removing composition of the present invention are curcumin diglucoside, rosemary extract, brown algae extract, and willow bark extract. These components work synergistically to achieve multiple effects such as oil control and unblocking, anti-inflammation, soothing and redness reduction, and barrier repair. They effectively prevent acne recurrence and acne scar residue. The overall formula balances high efficiency and gentleness, and has the advantages of fast onset, good effect, and gentle non-irritation. It is suitable for all types of acne-prone skin, even sensitive acne-prone skin, and has broad market application prospects. Detailed Implementation

[0025] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0026] The sources of the raw materials used in the following examples and comparative examples are as follows: Curcumin diglucoside: prepared by the preparation method of Example 5 of 2025103988177, a recombinant Escherichia coli for producing curcumin diglucoside and its application; Rosemary extract: purchased from Hunan Jinhan Pharmaceutical Co., Ltd.; Brown finger algae extract: from Guangzhou Bochi Biotechnology Co., Ltd.; Poplar and willow bark extract: from Guangzhou Bochi Biotechnology Co., Ltd.; Chlorella extract: from Guangzhou Bochi Biotechnology Co., Ltd.; Calendula extract: from Qiushi (Shandong) Import & Export Trading Co., Ltd.; Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0027] Examples 1-5 and Comparative Examples 1-8 The components and their weights of the anti-inflammatory and acne-reducing compositions of Examples 1-5 and Comparative Examples 1-8 are shown in Table 1. The total weight of each composition is the same.

[0028] Table 1. Formulations of various anti-inflammatory and acne-reducing compositions

[0029] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that the extract of *Phaeodactylum tricornutum* was replaced with extract of *Chlorella vulgaris*.

[0030] Comparative Example 8 The only difference between Comparative Example 8 and Example 1 is that the rosemary extract was replaced with calendula extract.

[0031] Test Example 1: Anti-inflammatory activity test of each composition (RAW264.7 cell model) (1) Cell culture After resuscitation, RAW264.7 mouse mononuclear macrophage cells were cultured in DMEM high-glucose medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37°C in a 5% CO2 incubator. Cells in the logarithmic growth phase were used for experiments.

[0032] (2) Sample preparation First, the rosemary extract in the anti-inflammatory and acne-removing compositions obtained in Examples 1-5 and Comparative Examples 1-8 was dissolved in a small amount (0.1% of the total solvent volume) of DMSO. Then, curcumin diglucoside, brown finger algae polysaccharide extract and white willow bark extract were added. The mixture was diluted with DMEM medium and sterilized through a 0.22 μm filter membrane to prepare a sample solution with a mass concentration of 1% (w / v).

[0033] (3) Cell seeding and grouping RAW264.7 cells were fed at a rate of 1×10⁻⁶. 5 Cells were seeded at a density of 1 mL per well in 12-well cell culture plates, with 1 mL of complete culture medium added to each well. The plates were then incubated at 37°C in a 5% CO2 incubator for 24 h.

[0034] The experiment was divided into the following groups: Blank control group: No LPS added, only an equal volume of culture medium added; Model control group (LPS): LPS (final concentration 1 μg / mL) was added. Solvent control group (Vehicle): LPS + equal amount of solvent; Positive control group (PC): LPS + dexamethasone (final concentration 10 μmol / L); Experimental group (sample): LPS + different combinations of embodiments.

[0035] (4) Drug administration and modeling After cell adhesion, the original culture medium was aspirated, and the cells were washed once with PBS. Both the experimental and control groups were pre-filled with 200 μL of fresh culture medium containing the corresponding test substance or solvent. After incubation for 2 h, except for the blank control group, LPS solution was added to each well to a final concentration of 1 μg / mL. The blank control group was added with an equal volume of serum-free culture medium. Incubation continued for 24 h.

[0036] (5) Collection and treatment of supernatant Collect the culture supernatant from each well, centrifuge at 3000 r / min for 10 min at 4℃, take the supernatant, and store it at −80℃ for later use, avoiding repeated freeze-thaw cycles.

[0037] (6) TNF-α content determination The mouse TNF-α ELISA kit was used, and the procedure was strictly followed according to the instructions. Set up standard sample wells, blank sample wells, and sample wells; Add biotin-labeled antibody and HRP-labeled streptavidin; TMB color development was performed, and the absorbance (OD value) was measured at a wavelength of 450 nm after the reaction was terminated. The correction wavelength was 570 nm. The concentration of TNF-α in each sample was calculated based on the standard curve.

[0038] (7) Calculation of anti-inflammatory activity Calculate the inhibition rate of the test substance against TNF-α using the following formula: ; In the formula: C LPS TNF-α concentration in the control group of the LPS model; C sample TNF-α concentration in the test substance treatment group; C blank TNF-α concentration in the blank control group.

[0039] Each group had 3 replicates, and the experiment was independently repeated 3 times. A higher TNF-α inhibition rate indicates stronger anti-inflammatory activity of the test substance. Data are shown in Table 2.

[0040] Test Example 2: Oil-controlling activity test of each composition (5α-reductase inhibition experiment) (1) Reagent preparation ① Testosterone (T) stock solution: Accurately weigh 28.8 mg of testosterone and place it in a 10 mL volumetric flask. Add anhydrous ethanol, sonicate to dissolve, and dilute to the mark to prepare a 10 mmol / L stock solution. Store at -20°C protected from light. Dilute to the working concentration (4 mmol / L) with reaction buffer immediately before use.

[0041] ② NADPH solution: Accurately weigh 42.5 mg of tetrasodium NADPH and place it in a 25 mL brown volumetric flask. Add pre-cooled 0.1 mol / L sodium phosphate buffer (pH 7.4) to completely dissolve the solution and bring it to a final volume to prepare a working solution with a concentration of 2 mmol / L. Prepare and use immediately, and operate on ice in the dark.

[0042] ③ Enzyme extraction buffer: Weigh 109.0 g of sucrose, 15.4 mg of dithiothreitol (DTT), and 7.16 g of disodium hydrogen phosphate (Na2HPO4·12H2O), dissolve them in an appropriate amount of ultrapure water, adjust the pH to 6.5 with phosphoric acid, and bring the volume to 1 L to obtain an enzyme extraction buffer containing 0.32 mol / L sucrose, 0.1 mmol / L DTT, and 20 mmol / L sodium phosphate. Store at 4 °C.

[0043] ④Reaction buffer solution: Weigh 15.4 mg DTT and 3.12 g sodium dihydrogen phosphate (NaH2PO4·2H2O), dissolve them in an appropriate amount of ultrapure water, adjust the pH to 6.8~7.0 with NaOH, and bring the volume to 1 L to obtain a reaction buffer containing 1 mmol / L DTT and 20 mmol / L sodium phosphate. Store at 4℃.

[0044] ⑤ Preparation of crude 5α-reductase solution: Male SD rats were sacrificed, and their skin tissue was quickly dissected, minced, and added to pre-cooled enzyme extraction buffer at a mass-to-volume ratio of 1:9. The mixture was homogenized under ice bath conditions. The homogenate was centrifuged at 12000 rpm for 30 min at 4°C, and the supernatant was collected as the crude 5α-reductase enzyme solution. After aliquoting, the solution was stored at -80°C, avoiding repeated freeze-thaw cycles. The protein concentration was determined before use.

[0045] (2) Sample processing First, the rosemary extract in the anti-inflammatory and acne-reducing compositions obtained in Examples 1-5 and Comparative Examples 1-8 was dissolved in DMSO (0.1% of the total solvent volume). Then, curcumin diglucoside, *Phaeodactylum tricornutum* polysaccharide extract, and *Salix babylonica* bark extract were added and diluted to 1 mg / mL with reaction buffer. After sterilization through a 0.22 μm filter membrane, the solution was set aside for use. Simultaneously, a reaction buffer (containing an equal volume of DMSO) was prepared as a solvent control.

[0046] (3) Experimental grouping Blank control group: The reaction buffer replaces the enzyme solution and sample and is used to subtract the background absorbance. Model control group: The reaction buffer replaced the sample and contained enzyme solution, testosterone, and NADPH; Solvent control group (Vehicle): Contains enzyme solution, testosterone, NADPH and an equal volume of DMSO solvent; Positive control group (PC): containing enzyme solution, testosterone, NADPH and 5α-reductase inhibitor (finasteride, final concentration 1 μmol / L). Experimental group (sample): contains enzyme solution, testosterone, NADPH and various combinations.

[0047] (4) Enzyme activity assay (ultraviolet spectrophotometry) The reaction system, with a total volume of 200 μL, was carried out in a 96-well UV plate using a continuous monitoring method. The sample addition sequence was as follows: Add 155 μL of reaction buffer and preheat at 37 °C for 5 min. Add 12 μL of testosterone solution (4 mmol / L) and mix well; Add 10 μL of NADPH solution (2 mmol / L) to start the reaction; Immediately add 20 μL of crude 5α-reductase solution and mix well; Finally, add 20 μL of sample solution (or control solution), mix quickly, and start timing.

[0048] At 37℃, the absorbance (OD) at 340nm wavelength was continuously monitored over 4 minutes using an ELISA reader. 340 The data is read every 30 seconds, showing the changes in the data. Each group has 3 duplicate holes.

[0049] (5) Data processing and calculation ① Plotting the NADPH standard curve: Prepare a series of NADPH standard solutions of different concentrations (0, 50, 100, 150, 200 μmol / L), measure the absorbance at 340 nm, plot the concentration-absorbance standard curve, and calculate the regression equation.

[0050] ② Definition of enzyme activity unit: Under conditions of 37°C and pH 6.8, the amount of enzyme required to catalyze the oxidation of 1 μmol NADPH per minute is defined as one unit of enzyme activity (U).

[0051] ③ Inhibition rate calculation: Based on the NADPH standard curve, calculate the reaction rate (ΔOD / min) of each reaction system within the linear range, and then convert it to enzyme activity. Calculate the 5α-reductase inhibition rate using the following formula: ; V sample : Reaction rate of the experimental group (μmol / min); V model : Reaction rate of the model control group (μmol / min); V blank : Reaction rate of the blank control group (μmol / min).

[0052] Each experiment was repeated independently in 3 instances. A higher inhibition rate indicates a stronger oil-controlling (anti-sebum secretion) activity of the test substance. Data are shown in Table 2.

[0053] Table 2. TNF-α inhibition rate and 5α-reductase inhibition rate of each composition

[0054] As shown in Table 2, the data from Examples 1-5 and Comparative Examples 5-6 indicate that when the weight ratio of curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract, and *Salix babylonica* bark extract in the composition is within the range of (3-6):(2-4):(4-6):(0.8-2), the composition exhibits higher TNF-α inhibition and 5α-reductase inhibition rates, indicating better anti-inflammatory and oil-controlling effects. Conversely, when the weight ratio of curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract, and *Salix babylonica* bark extract is outside the range of (2-8):(1-5):(3-7):(0.5-3), the anti-inflammatory and oil-controlling effects of the composition are significantly reduced.

[0055] Based on the data from Example 1, Comparative Examples 1-4 and Comparative Examples 7-8, it can be seen that if a certain ingredient is missing from the composition or if a certain ingredient is replaced, the TNF-α inhibition rate and 5α-reductase inhibition rate of the composition are significantly reduced. This indicates that curcumin diglucoside, rosemary extract, brown finger algae extract and white poplar bark extract can synergistically achieve the effects of oil control, unblocking and anti-inflammation.

[0056] Application Example 1-5 and Comparative Application Example 1-9 The present invention provides an anti-inflammatory acne-removing essence in application examples and comparative application examples. The components (weight percentage) of the essence are shown in Table 3. The anti-inflammatory acne-removing compositions used in application examples 1-5 are the anti-inflammatory acne-removing compositions prepared in examples 1-5, and the anti-inflammatory acne-removing compositions used in comparative application examples 1-8 are the anti-inflammatory acne-removing compositions prepared in comparative examples 1-8. In addition, a comparative application example 9 (blank application example) is set up. The only difference between comparative application example 9 and application example 1 is that no anti-inflammatory acne-removing composition is added in comparative application example 9, and deionized water is used to make up the missing amount. The preparation method is the same as that of application example 1.

[0057] Table 3. Serum Formulas for Each Group

[0058] The preparation method of the above-mentioned anti-inflammatory and acne-reducing serum includes the following steps: S1. Add the chelating agent and 1 / 2 deionized water to the reaction vessel, stir to dissolve, then add the humectant and antioxidant, heat to 80°C, keep warm and stir to obtain the aqueous phase; S2. Add a thickener to the aqueous phase, stir evenly, then cool to 40°C, add the anti-inflammatory and acne-removing composition, preservative and remaining deionized water, stir until evenly dissolved to obtain the liquid. S3. Add pH adjuster to the liquid, stir, filter and discharge to obtain the anti-inflammatory and acne-removing essence.

[0059] Test Example 3: Safety Tests of Various Serums (Patch Test) Thirty volunteers were selected. The serums used in Examples 1-5 and the control examples 1-7 were applied to the flexor surfaces of both forearms of the subjects for 24 hours. All plaster applicators were then gently and completely removed, and any remaining sample was wiped away with a cotton swab. After 30 minutes of rest until the pressure marks on the skin completely disappeared, the skin reaction at each point was observed and recorded visually for the first time. At 24 hours and 48 hours after plaster applicator removal, the skin condition of all subjects was observed a second and third time, and photographs were taken for record-keeping.

[0060] The results showed that at three observation time points (30 min, 24 h, and 48 h after patch removal), all tested areas of the volunteers who applied the serums from Examples 1-5 and Comparative Examples 1-9 showed a grade 0 reaction, with no skin irritation symptoms such as erythema, edema, infiltration, papules, vesicles, itching, or stinging. The blank saline control group showed no abnormal reactions. The anti-inflammatory and acne-reducing serums prepared in Examples 1-5 and Comparative Examples 1-7 of this invention were verified through a 24-hour human patch test to be non-irritating to healthy human skin, with a mild formula system, low risk of allergic reactions, and suitable for long-term use on sensitive facial acne-prone skin.

[0061] Test Example 4: Human Efficacy Test of Various Serums 1. Subjects and Grouping Seventy healthy male or female volunteers aged 18-45 with mild to moderate acne (blackheads and papules) on their faces were randomly divided into 14 groups of 5 people each.

[0062] Inclusion criteria: The severity of facial acne meets the quantitative inclusion criteria for mild to moderate acne (refer to the "Chinese Acne Treatment Guidelines"): Only non-inflammatory comedones (whiteheads, blackheads) and inflammatory papules are present on the face, without nodules, cysts or other severe acne lesions; 10-35 non-inflammatory comedones and 3-15 inflammatory papules are present on one side of the cheek and forehead, without large areas of redness, swelling or purulent lesions.

[0063] Exclusion criteria: Pregnant or breastfeeding women; those with known allergies to cosmetic ingredients; those who have taken orally or topically acne medications such as retinoids, antibiotics, or corticosteroids within the past two weeks.

[0064] Grouping: The anti-inflammatory acne-reducing serums used in Application Examples 1-5 and Control Application Examples 1-7 were used respectively.

[0065] 2. Testing Process After cleansing their faces, participants applied the corresponding sample to their faces twice daily, morning and evening, using 1 mL each time. Other functional skincare products were discontinued during the trial period.

[0066] 3. Detection Indicators and Calculation Formulas Before use (day 0) and 14 days after sample use, blind testing was conducted by the same trained researcher in a constant temperature and humidity environment (temperature 22±1℃, humidity 50±5%). (1) Improvement rate of acne / papules Two dermatologists conducted a blinded assessment, counting facial comedones (non-inflammatory) and papules (inflammatory).

[0067] Calculation formula: Acne / papule improvement rate (%) = [(Number before use - Number after use) / Number before use] × 100%. The higher the acne / papule improvement rate, the better the acne or papule reduction effect.

[0068] (2) Skin color a-value improvement rate The VISIA-CR facial image analysis system was used to measure fixed areas on both cheeks and the forehead. The a* value represents the red-green axis; a higher value indicates more severe erythema / swelling.

[0069] Calculation formula: The improvement rate of a value (%) is calculated as follows: [(a value before use - a value after use) / a value before use] × 100%. The higher the a value improvement rate, the more significant the reduction of skin erythema and the better the anti-inflammatory and redness-reducing effect.

[0070] (3) Oil improvement rate Using Sebumeter ® The SM815 sebum analyzer measures and records the amount of sebum secreted per unit time (μg / cm²) on the nose and T-zone of the forehead.

[0071] Calculation formula: Oil control improvement rate (%) = [(Oil amount before use - Oil amount after use) / Oil amount before use] × 100%; the higher the oil control improvement rate, the more significant the reduction in sebum secretion and the better the oil control effect. See Table 4 for data.

[0072] Table 4. Human efficacy test data for each serum Application Example 1 33.8 32.3 26.1 Application Example 2 31.4 31.7 25.3 Application Example 3 32.1 31.5 25.8 Application Example 4 30.2 30.8 24.6 Application Example 5 31.6 31.5 25.4 Comparative Application Example 1 15.3 19.3 13.0 Comparative Application Example 2 25.1 21.6 16.1 Comparative Application Example 3 19.7 25.4 14.6 Comparative Application Example 4 17.3 21.1 15.5 Comparative Application Example 5 22.5 25.8 19.6 Comparative Application Example 6 24.1 22.0 21.4 Comparative Application Example 7 21.4 24.6 16.9 Comparative Application Example 8 23.6 21.4 18.7 Comparative Application Example 9 8.5 5.1 2.3 As shown in Table 4, the improvement rates of acne / papules, skin a-value, and oiliness in volunteers using Application Examples 1-5 and Comparative Application Examples 1-8 were all higher than those in Comparative Application Example 9 (blank application example). This indicates that the addition of the anti-inflammatory and acne-removing composition is an important factor affecting the oil-controlling, acne-removing, anti-inflammatory, and redness-reducing effects of the serum.

[0073] Combining the data from Application Examples 1-5 and Comparative Application Examples 5-6, it can be seen that when the weight ratio of curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract, and *Salix babylonica* bark extract in the composition added to the serum is within the range of (3-6):(2-4):(4-6):(0.8-2), the serum exhibits better oil-controlling, acne-reducing, and anti-inflammatory / redness-reducing effects. However, if the weight ratio of curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract, and *Salix babylonica* bark extract in the composition is not within the range of (2-8):(1-5):(3-7):(0.5-3), the serum's oil-controlling, acne-reducing, and anti-inflammatory / redness-reducing effects are significantly reduced, demonstrating the necessity of limiting the range.

[0074] Combining the data from Application Example 1, Comparative Application Examples 1-4, and Comparative Application Examples 7-8, it can be seen that when a certain ingredient is missing from the composition of the serum or is replaced, the improvement rate of acne / papules, the improvement rate of skin a-value, and the improvement rate of oiliness are significantly lower than in Application Example 1. This indicates that curcumin diglucoside, rosemary extract, *Phaeodactylum tricornutum* extract, and *Saussurea costatum* bark extract can synergistically achieve multiple effects such as oil control and unblocking, anti-inflammation, soothing and redness reduction, and barrier repair, effectively preventing acne recurrence and acne scar residue. Moreover, after the composition was made into various serums, no precipitation or discoloration problems caused by curcumin diglucoside were observed, and it is safe and non-irritating, suitable for various acne-prone skin types. This verifies the feasibility and safety of using it as a core active ingredient in water-based serum systems, overcoming the formulation application challenges of free curcumin.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-inflammatory and acne-reducing composition containing curcumin diglucoside, characterized in that, It includes the following components: curcumin diglucoside, rosemary extract, brown finger algae extract and white willow bark extract; the weight ratio of curcumin diglucoside, rosemary extract, brown finger algae extract and white willow bark extract is (2-8):(1-5):(3-7):(0.5-3).

2. The anti-inflammatory and acne-reducing composition containing curcumin diglucoside as described in claim 1, characterized in that, The weight ratio of curcumin diglucoside, rosemary extract, brown finger algae extract and white poplar bark extract is (3-6):(2-4):(4-6):(0.8-2).

3. The anti-inflammatory and acne-reducing composition containing curcumin diglucoside as described in claim 1, characterized in that, The weight ratio of curcumin diglucoside, rosemary extract, brown finger algae extract, and willow bark extract is 5:3:5:

1.

4. The use of the anti-inflammatory and acne-removing composition containing curcumin diglucoside according to any one of claims 1-3 in the preparation of acne-removing cosmetics.

5. The application as described in claim 4, characterized in that, The acne-removing cosmetics include serums, creams, gels, masks, or sprays; the amount of the anti-inflammatory acne-removing composition containing curcumin diglucoside added is 0.5-3%.

6. An anti-inflammatory and acne-reducing essence, characterized in that, Including the following raw materials by weight percentage: 0.5-3% of the anti-inflammatory and acne-reducing composition containing curcumin diglucoside according to any one of claims 1-3, 3-8% of moisturizer, 0.05-0.15% of antioxidant, 0.01-0.1% of chelating agent, 0.15-0.6% of thickener, 0.01-0.1% of pH adjuster and 0.08-0.3% of preservative, with the balance being deionized water.

7. The anti-inflammatory and acne-removing essence as described in claim 6, characterized in that, The raw material is selected from at least one of (I)-(VI): (I) The moisturizer is at least one of butylene glycol, glycerin, 1,2-hexanediol, propylene glycol, 1,2-pentanediol and sodium hyaluronate; (II) The antioxidant is at least one of p-hydroxyacetophenone, tea polyphenols, tocopherol, tocopheryl acetate and ferulic acid; (III) The chelating agent is disodium EDTA; (IV) The thickener is at least one of the following: acrylate / C10-30 alkanol acrylate crosspolymer, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, carbomer, xanthan gum, sodium polyacrylate and hydroxyethyl cellulose; (V) The pH adjuster is triethanolamine and / or arginine; (VI) The preservative is at least one of phenoxyethanol, ethylhexylglycerin, octyl glycol, and 1,2-hexanediol.

8. The preparation method of the anti-inflammatory and acne-removing essence as described in claim 6, characterized in that, Includes the following steps: S1. Add the chelating agent and some deionized water to the reaction vessel, stir to dissolve, then add the humectant and antioxidant, heat to 75-85℃, keep warm and stir to obtain the aqueous phase; S2. Add a thickener to the aqueous phase, stir evenly, then cool to 40-45℃, add the anti-inflammatory and acne-removing composition, preservative and remaining deionized water, stir until evenly dissolved to obtain the liquid. S3. Add pH adjuster to the liquid, stir, filter and discharge to obtain the anti-inflammatory and acne-removing essence.

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