Preparation method of bio-organic fertilizer for preventing tobacco root rot and nematode disease

Abstract

The application provides a preparation method of a biological organic fertilizer for preventing tobacco root rot and nematode disease, and relates to the technical field of organic fertilizer.The biological organic fertilizer for preventing tobacco root rot and nematode disease is prepared from the following raw materials in parts by mass: 10-12 parts of a compound microbial agent, 8-9 parts of silicon-rich rings, 87-89 parts of rotten and fermented agricultural waste, and 1-3 parts of a compound fatty acid; the compound microbial agent is prepared from Bacillus velezensis, Bacillus subtilis subsp., Bacillus pumilus and Streptomyces microflavus in equal proportions; and the rotten and fermented agricultural waste is prepared from one or a mixture of several of the following: plant straw, grass, tree branches and kitchen garbage after fermentation.The organic fertilizer can prevent tobacco root rot and nematode disease, and can supply nutrients for tobacco.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer technology, specifically to a method for preparing a bio-organic fertilizer that can prevent both tobacco root rot and nematode disease. Background Technology

[0002] Tobacco is an important economic crop in my country, with its annual production and sales accounting for approximately one-third of the global total. In recent years, Fusarium root rot of tobacco has been reported across the country, impacting the development of the tobacco industry.

[0003] Tobacco is a plant belonging to the genus Nicotiana in the family Solanaceae, and is an annual or short-lived perennial herb. Tobacco can be used as a raw material for the tobacco industry; it can also be used as an insecticide. The nicotine in tobacco has a broad insecticidal spectrum, is fast-acting, easily biodegradable, safe for most crops, and leaves little or no residue. It can kill more than two hundred kinds of pests, including Lepidoptera, Diptera, Coleoptera, and Hemiptera. It also has medicinal uses, as an anesthetic, diaphoretic, sedative, and emetic. Tobacco cyst nematode disease was first reported by Lownsherry in Connecticut, USA, in 1954. Currently, tobacco cyst nematode disease only occurs sporadically in some tobacco-growing areas of Shandong and Henan provinces, causing relatively mild damage. Tobacco root-knot nematode disease occurs in all major tobacco-growing areas worldwide, with severe damage in tropical, subtropical, and warm temperate regions. Tobacco root-knot nematodes are relatively common in Yunnan Province, my country, while localized severe outbreaks occur in tobacco-growing areas of Guangxi, Guizhou, Sichuan, Chongqing, Hubei, Henan, Shaanxi, and Shandong provinces. Root-knot nematodes can cause combined infections with root rot, exacerbating the damage.

[0004] Fusarium root rot in tobacco has gradually risen from a secondary disease to a major disease in some tobacco-growing areas, causing serious economic losses to tobacco production. Currently, there are relatively few reports on field screening of pesticides for controlling Fusarium root rot in tobacco; therefore, screening for effective pesticides is of great significance. Previously commonly used pesticides for controlling root and stem diseases (such as metalaxyl, dimethomorph, thiram, and zineb) are not only ineffective against Fusarium root rot in tobacco, but have also shown resistance to other common root and stem diseases of tobacco after years of use, so their use is not advisable. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a method for preparing a bio-organic fertilizer that can prevent both tobacco root rot and nematode diseases, thus solving the problem of unsatisfactory control effects against tobacco Fusarium root rot.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a bio-organic fertilizer that prevents both tobacco root rot and nematode disease, comprising the following raw materials in parts by weight:

[0007] 10-12 parts of compound microbial inoculant, 8-9 parts of silicon-rich ring, 87-89 parts of fermented agricultural waste, and 1-3 parts of compound fatty acids;

[0008] The compound microbial agent package is composed of equal proportions of Bacillus belye, Bacillus subtilis subtype, Bacillus pumilus, and Streptomyces flavus;

[0009] Fermented agricultural waste consists of one or more of the following: fermented plant stalks, grass, branches, and kitchen waste.

[0010] The preferred method for preparing complex fatty acids is as follows:

[0011] The oil cake or oil cake raw material is fully fermented. After fermentation, 60% saturated fatty acids and 40% unsaturated fatty acids are added to form a complex fatty acid. The saturated fatty acid is palmitic acid and the unsaturated fatty acid is oleic acid.

[0012] Preferably, the mixture contains 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 87 parts of fermented agricultural waste, and 1 part of compound fatty acid.

[0013] Preferably, the mixture contains 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 89 parts of fermented agricultural waste, and 3 parts of compound fatty acid.

[0014] The preferred method for preparing Bacillus belyss is as follows:

[0015] Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Bacillus belye into the culture medium and culture to obtain Bacillus belye.

[0016] The preferred method for preparing the Bacillus subtype is as follows:

[0017] Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate the Bacillus subtilis subtype into the culture medium and culture to obtain the Bacillus subtilis subtype.

[0018] The preferred method for preparing Bacillus pumilus is as follows:

[0019] Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Bacillus pumilus into the culture medium and culture to obtain Bacillus pumilus.

[0020] The preferred method for preparing Streptomyces flavus is as follows:

[0021] Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Streptomyces flavus into the culture medium and culture to obtain Streptomyces flavus.

[0022] Preferably, a method for preparing a bio-organic fertilizer that prevents both tobacco root rot and nematode disease includes the following steps:

[0023] The compound microbial agent, silicon-rich ring, decomposed and fermented agricultural waste, and compound fatty acids are thoroughly mixed and stirred evenly to obtain a high-fat fatty acid bio-organic fertilizer that can prevent tobacco root rot and nematode disease. The organic fertilizer is then tested for nutritional ratio and packaged.

[0024] Preferably, a bio-organic fertilizer that can prevent both tobacco root rot and nematode diseases is used for the prevention and control of Fusarium, root-knot nematodes and cyst nematodes and their larvae in tobacco cultivation, for the supplementation of fatty acid nutrients in tobacco cultivation, and for fertilization in tobacco cultivation.

[0025] This invention provides a method for preparing a bio-organic fertilizer that simultaneously prevents tobacco root rot and nematode diseases. It has the following beneficial effects:

[0026] This invention utilizes bio-organic fertilizer for root treatment, exhibiting strong control over Fusarium, root-knot nematodes, cyst nematodes, and their larvae. It also significantly inhibits the hatching of egg sacs, solving the problem of tobacco nematode control. The resulting biological control agent shows promising application prospects in controlling tobacco root diseases. It overcomes the problems of existing ordinary organic fertilizers used on tobacco, which have excessively low fatty acid content, failing to meet the needs of tobacco leaf growth and exhibiting insufficient decomposition and absorption. The core technology of this invention is the use of a compound fatty acid mixture of 60% saturated fatty acids and 40% unsaturated fatty acids as an additive, allowing for better absorption and utilization by crops, promoting plant growth, and improving tobacco leaf quality. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the present invention. Detailed Implementation

[0028] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1:

[0030] like Figure 1 As shown, this embodiment of the invention provides a bio-organic fertilizer that prevents both tobacco root rot and nematode disease, composed of the following raw materials in parts by weight:

[0031] This formula contains 10 parts of compound microbial agent, 8 parts of silicon-rich ring, 87 parts of fermented agricultural waste, and 1 part of compound fatty acid. It can meet the specific needs of tobacco root rot control and better control this disease.

[0032] The compound microbial agent package is composed of equal proportions of Bacillus vesiculosus, Bacillus subtype, Bacillus pumilus, and Streptomyces flavus. It is added to high fatty acid organic fertilizer in proportion to form the characteristics of resistance to root rot and nematodes.

[0033] Fermented agricultural waste is a mixture of one or more of the following: fermented plant stalks, grass, branches, and kitchen waste. It is a waste utilization method that is low in cost and environmentally friendly.

[0034] The preparation method of complex fatty acids is as follows:

[0035] The raw materials, such as oil cake or oil cake, are fully fermented. After fermentation, 60% saturated fatty acids and 40% unsaturated fatty acids are added to form a complex fatty acid. The saturated fatty acid is palmitic acid, and the unsaturated fatty acid is oleic acid. It is easy to obtain and has a low cost.

[0036] The formula contains 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 87 parts of fermented agricultural waste, and 1 part of compound fatty acid. This formula is mainly effective in preventing tobacco root rot and tobacco nematode disease.

[0037] The formula contains 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 89 parts of fermented agricultural waste, and 3 parts of compound fatty acids. This formula mainly provides nutrients and has the effect of killing tobacco nematodes.

[0038] The preparation method of Bacillus belyssus is as follows:

[0039] A culture medium is prepared by mixing 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate. Bacillus belye is then inoculated into the culture medium to obtain Bacillus belye. This method has low culture costs, high reproductive activity, and is beneficial for the preparation of organic fertilizer.

[0040] The preparation method of Bacillus subtilis subtype is as follows:

[0041] A culture medium is prepared by mixing 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate. Then, the Bacillus subtilis subtype is inoculated into the culture medium and cultured to obtain the Bacillus subtilis subtype. The culture cost is low, the reproduction activity is high, and it is beneficial for the preparation of organic fertilizer.

[0042] The preparation method of Bacillus pumilus is as follows:

[0043] A culture medium is prepared by mixing 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate. Then, *Bacillus pumilus* is inoculated into the culture medium and cultured to obtain *Bacillus pumilus*. This method has low culture costs, high reproductive activity, and is beneficial for the preparation of organic fertilizer.

[0044] The preparation method of Streptomyces flavus is as follows:

[0045] A culture medium is prepared by mixing 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate. Then, *Streptomyces flavus* is inoculated into the culture medium and cultured to obtain *Streptomyces flavus*. The culture cost is low, the reproduction activity is high, and it is beneficial for the preparation of organic fertilizer.

[0046] Example 2:

[0047] Reference Figure 1 As shown: This embodiment of the invention provides a method for preparing a bio-organic fertilizer that prevents both tobacco root rot and nematode disease, comprising the following steps:

[0048] The compound microbial agent, silicon-rich ring, decomposed and fermented agricultural waste, and compound fatty acids are thoroughly mixed and stirred evenly to obtain a high-fat fatty acid bio-organic fertilizer that can prevent tobacco root rot and nematode disease. The organic fertilizer is then tested for nutritional ratio and packaged.

[0049] Example 3:

[0050] Reference Figure 1 As shown, this embodiment of the invention provides an application of a bio-organic fertilizer that can prevent both tobacco root rot and nematode diseases. It is used to control Fusarium, root-knot nematodes, and cyst nematodes and their larvae in tobacco cultivation, to supplement fatty acid nutrients in tobacco cultivation, and to fertilize tobacco plants.

[0051] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A bio-organic fertilizer that prevents both tobacco root rot and nematode disease, characterized in that: It consists of the following parts by weight of raw materials: 10-12 parts of compound microbial inoculant, 8-9 parts of silicon-rich ring, 87-89 parts of fermented agricultural waste, and 1-3 parts of compound fatty acids; The compound microbial agent package is composed of equal proportions of Bacillus belye, Bacillus subtilis subtype, Bacillus pumilus, and Streptomyces flavus; Fermented agricultural waste consists of one or more of the following: fermented plant stalks, grass, branches, and kitchen waste.

2. The bio-organic fertilizer that prevents both tobacco root rot and nematode disease according to claim 1, characterized in that: The preparation method of complex fatty acids is as follows: The oil cake or oil cake raw material is fully fermented. After fermentation, 60% saturated fatty acids and 40% unsaturated fatty acids are added to form a complex fatty acid. The saturated fatty acid is palmitic acid and the unsaturated fatty acid is oleic acid.

3. The bio-organic fertilizer that prevents both tobacco root rot and nematode disease according to claim 1, characterized in that: 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 87 parts of fermented agricultural waste, and 1 part of compound fatty acid.

4. The bio-organic fertilizer for preventing both tobacco root rot and nematode disease according to claim 1, characterized in that: 10 parts of compound microbial inoculant, 8 parts of silicon-rich ring, 89 parts of fermented agricultural waste, and 3 parts of compound fatty acids.

5. The bio-organic fertilizer according to claim 1, which simultaneously prevents tobacco root rot and nematode disease, is characterized in that: The preparation method of Bacillus belyssus is as follows: Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Bacillus belye into the culture medium and culture to obtain Bacillus belye.

6. The bio-organic fertilizer according to claim 1, which simultaneously prevents tobacco root rot and nematode disease, is characterized in that: The preparation method of Bacillus subtilis subtype is as follows: Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate the Bacillus subtilis subtype into the culture medium and culture to obtain the Bacillus subtilis subtype.

7. The bio-organic fertilizer according to claim 1, which simultaneously prevents tobacco root rot and nematode disease, is characterized in that: The preparation method of Bacillus pumilus is as follows: Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Bacillus pumilus into the culture medium and culture to obtain Bacillus pumilus.

8. The bio-organic fertilizer according to claim 1, which simultaneously prevents tobacco root rot and nematode disease, is characterized in that: The preparation method of Streptomyces flavus is as follows: Mix 50-80 parts corn starch, 10-15 parts soybean flour, 0.1-0.2 parts molasses solution, 0.01-0.03 parts ammonium sulfate, 0.01-0.03 parts magnesium sulfate, 0.02-0.05 parts manganese sulfate, and 0.05-0.1 parts potassium dihydrogen phosphate to form a culture medium. Then, inoculate Streptomyces flavus into the culture medium and culture to obtain Streptomyces flavus.

9. The method for preparing a bio-organic fertilizer that prevents both tobacco root rot and nematode disease according to claim 1, characterized in that: Includes the following steps: The compound microbial agent, silicon-rich ring, decomposed and fermented agricultural waste, and compound fatty acids are thoroughly mixed and stirred evenly to obtain a high-fat fatty acid bio-organic fertilizer that can prevent tobacco root rot and nematode disease. The organic fertilizer is then tested for nutritional ratio and packaged.

10. The application of a bio-organic fertilizer that simultaneously prevents tobacco root rot and nematode disease according to claim 1, characterized in that: Used for the prevention and control of Fusarium, root-knot nematodes, and cyst nematodes and their larvae in tobacco cultivation; used as a fatty acid nutrient supplement in tobacco cultivation; used as fertilizer in tobacco cultivation.

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