Snow-white aspergillus strain ggnsj008 with resistance to puccinia hordei and application thereof

Abstract

The application provides a snow-white aspergillus strain GGNSJ008 with anti-pucciniastrafesicola and an application thereof, and belongs to the technical field of functional microorganisms. Aspergillus niveus The strain GGNSJ008 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 42606. The strain GGNSJ008 is isolated from pueraria endophytic bacteria, has inhibitory activity on 10 common pathogenic fungi, and has the most obvious inhibitory effect on fuscum acuminatum, followed by fusarium graminearum, fusarium verticillioides and guangxi glomerella. It can be seen that the strain GGNSJ008 has effective inhibitory activity on various fungal diseases and has good broad-spectrum antibacterial property, and can be used for preventing and treating diseases caused by the above pathogenic fungi.

Description

Technical Field

[0001] This application belongs to the field of functional microbial technology, specifically relating to a species of Aspergillus snow white that has resistance to the pathogenic fungus of kudzu root rot (Aspergillus snow white). Aspergillus nivus ) strain GGNSJ008 and its application. Background Technology

[0002] As a plant used for both food and medicine, kudzu root possesses significant nutritional and medicinal value. Pharmacological studies have shown that kudzu root has effects such as lowering blood sugar and lipids, anti-inflammation, anti-oxidation, and lowering blood pressure. However, kudzu root cultivation is prone to problems such as soil degradation, water pollution, decreased biodiversity, and increased resistance to pathogens, leading to frequent disease outbreaks and resulting in reduced yield and lower quality.

[0003] Endophytic fungi are crucial for promoting plant health. Studies have shown that they not only interact directly with plants but also act as hubs to regulate the structure and function of the rhizosphere and endophytic microbial communities, indirectly affecting plant health. However, there are currently no reports on the control of kudzu root diseases by endophytic fungi. Summary of the Invention

[0004] The purpose of this invention is to provide a *Aspergillus oryzae* strain GGNSJ008 that is resistant to kudzu root rot pathogens. It has antibacterial activity against dozens of common pathogens, including *Fusarium argentis*, *Fusarium graminearum*, and *Fusarium verticillatum*, and can be used in the preparation of broad-spectrum biocontrol agents and in the prevention and control of kudzu root diseases.

[0005] This invention provides a white Aspergillus ( Aspergillus nivus The strain GGNSJ008 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42606.

[0006] The present invention provides a biocontrol agent comprising the Aspergillus oryzae strain GGNSJ008 or a culture of the Aspergillus oryzae strain GGNSJ008.

[0007] This invention provides a compound microbial fertilizer, comprising the Aspergillus oryzae strain GGNSJ008, the biocontrol agent or a culture of the Aspergillus oryzae strain GGNSJ008, and a biofertilizer; The mass ratio of the Aspergillus oryzae strain GGNSJ008, the biocontrol agent, or the culture of the Aspergillus oryzae strain GGNSJ008 to the biofertilizer is 1~5:1~20.

[0008] Preferably, the bio-fertilizer includes at least one of the following: well-rotted poultry and livestock manure organic fertilizer, well-rotted agricultural waste organic fertilizer, and well-rotted medicinal residue or fungal residue organic fertilizer.

[0009] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008, the biocontrol agent, the culture of the *Aspergillus spp.* strain GGNSJ008, or the compound microbial fertilizer in controlling plant diseases caused by at least one of the following pathogenic fungi: *Fusarium argenteum* (…). Fusarium acuminata Fusarium solani () Fusarium solani ), the pathogen of kudzu leaf spot, *Streptococcus parviflora* ( Alternaria alternata Fruit anthrax ( Colletotrichum fructicola ), Sclerotinia sclerotiorum ( Sclerotinia sclerotia ), Karst anthrax bacteria ( Colletotrichum karsti Fusarium graminearum ( ), Fusarium grasses Fusarium oxysporum ( Fusarium oxysporum ), Fusarium verticillata ( Fusarium whorl-shaped ) and multi-master spirochete ( Corynespora cassicola ).

[0010] Preferably, the plant includes traditional Chinese medicine and / or crops.

[0011] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008, the biocontrol agent, the culture of the *Aspergillus spp.* strain GGNSJ008, or the compound microbial fertilizer in kudzu cultivation or the control of kudzu diseases. The kudzu diseases include at least one of the following pathogenic fungi: *Fusarium racifolium*, *Fusarium solani*, *Streptococcus spp.* (the kudzu leaf spot pathogen), *Anthracis chinensis*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium oxysporum*, and *Cyclocarya pallida*.

[0012] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008 and / or a culture of the *Aspergillus spp.* strain GGNSJ008 in the preparation of a broad-spectrum antibacterial agent. The broad-spectrum antibacterial agent targets at least one of the following pathogenic fungi: *Fusarium argentis*, *Fusarium solani*, *Streptococcus spp.*, *Anthracis ciliatus*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium verticillatum*, and *Cyclocarya pallida*.

[0013] This invention provides a method for preventing and controlling plant diseases, which involves applying the Aspergillus oryzae strain GGNSJ008, the biocontrol agent, a culture of the Aspergillus oryzae strain GGNSJ008, or the compound microbial fertilizer.

[0014] Preferably, the method of application includes root irrigation.

[0015] This invention provides a *Aspergillus spp.* strain GGNSJ008, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42606. The strain GGNSJ008 described in this invention was isolated from the rhizosphere of kudzu root and identified as *Aspergillus spp.* by morphological and molecular identification. Aspergillus nivus The strain GGNSJ008 was subjected to plate confrontation experiments against 10 pathogenic fungi. The results showed that strain GGNSJ008 had antibacterial activity against all 10 pathogenic fungi, with the most significant inhibitory effect against *Fusarium argentis*, at an inhibition rate of 31.76%; followed by *Fusarium graminearum*, *Fusarium verticillatum*, and *Anthracnose karstii*, with inhibition rates of 22.22%, 20.94%, and 20%, respectively. Therefore, the strain GGNSJ008 of this invention has effective antibacterial activity against a variety of fungal diseases, exhibiting good broad-spectrum antibacterial properties, and can be used to control diseases caused by the aforementioned pathogenic fungi. Attached Figure Description

[0016] Figure 1 The morphological identification results of Aspergillus niger strain GGNSJ008 are shown in Figure a, where a is the colony morphology characteristic image, b is the conidia morphology characteristic image, and c is the conidiophore morphology characteristic image. Figure 2 Phylogenetic tree of Aspergillus niger strain GGNSJ008; Figure 3 The image shows the antifungal effect of Aspergillus niger strain GGNSJ008 against kudzu pathogens, where a: Fusarium raffins; b: Fusarium solani; c: Fusarium oxysporum; d: Fusarium verticillatum; e: *Cyclocarya paliurus*; f: *Anthracnose citrinum*; g: *Fusarium graminearum*; h: *Sclerotinia sclerotiorum*; j: *Anthracnose karstii*; k: *Alternaria alternata*; scale bar is 1 cm.

[0017] Information on the preservation of biological materials Aspergillus snow white ( Aspergillus nivus Strain GGNSJ008 was deposited on April 8, 2026, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 42606. Detailed Implementation

[0018] This invention provides a white Aspergillus ( Aspergillus nivus The strain GGNSJ008 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42606.

[0019] In this invention, strain GGNSJ008 was isolated and screened from kudzu root. The morphological characteristics of strain GGNSJ008 are as follows: colonies cultured on PDA medium for 7 days reach a diameter of 2.3 cm, are irregularly round, velvety grayish-white, wrinkled, with a yellowish-brown back; conidial heads are densely spherical or cylindrical; conidiophores are 4-9 µm in diameter; vesicles are flask-shaped with a diameter of 10-20 µm; conidial spheroids have slightly rough walls and a diameter of 2.0-3.5 µm. The nucleotide sequences of the rDNA-ITS, RPB2, CAL, and TUB genes of strain GGNSJ008 are shown in SEQ ID NO:1-SEQ ID NO:4, respectively. Phylogenetic tree construction shows that strain GGNSJ008 clusters with *Aspergillus spp.*, while other *Aspergillus* fungi form independent branches. Therefore, strain GGNSJ008 is identified as *Aspergillus spp.* Aspergillus nivus ).

[0020] The present invention provides a biocontrol agent comprising the Aspergillus oryzae strain GGNSJ008 or a culture of the Aspergillus oryzae strain GGNSJ008.

[0021] In this invention, the preferred method for preparing the culture of the Aspergillus spp. strain GGNSJ008 is to inoculate the activated Aspergillus spp. strain GGNSJ008 into PDA liquid medium and culture it to obtain a culture. The culture contains the cells of the Aspergillus spp. strain GGNSJ008 and a secondary culture of the Aspergillus spp. strain GGNSJ008.

[0022] In this invention, the biocontrol agent preferably further includes excipients. There are no particular limitations on the type of excipients; any excipients well-known in the art can be used. The mass percentage of the *Aspergillus spp.* strain GGNSJ008 or the culture of *Aspergillus spp.* strain GGNSJ008 is preferably 5%~95%, can be 10%~90%, can be 15%~85%, can be 20%~80%, and further can be 30%~50%. There are no particular limitations on the preparation method of the biocontrol agent; any preparation method well-known in the art can be used.

[0023] This invention provides a compound microbial fertilizer, comprising the Aspergillus spp. strain GGNSJ008, the biocontrol agent or a culture of the Aspergillus spp. strain GGNSJ008, and a biofertilizer; the mass ratio of the Aspergillus spp. strain GGNSJ008, the biocontrol agent or a culture of the Aspergillus spp. strain GGNSJ008, to the biofertilizer is 1~5:1~20.

[0024] In this invention, the preferred mass ratio of the *Aspergillus spp.* strain GGNSJ008 or its culture to the bio-fertilizer is 1-3:5-10, but can also be 1:10, 1:5, or 1:1. The bio-fertilizer preferably includes at least one of the following: well-rotted poultry and livestock manure organic fertilizer, well-rotted agricultural waste organic fertilizer, or well-rotted medicinal residue or fungal residue organic fertilizer. The addition of the bio-fertilizer provides the nutrients required for plant growth, while the *Aspergillus spp.* strain GGNSJ008 or its culture can play a role in the control of various diseases, thereby improving plant growth.

[0025] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008, the biocontrol agent, the culture of the *Aspergillus spp.* strain GGNSJ008, or the compound microbial fertilizer in controlling plant diseases caused by at least one of the following pathogenic fungi: *Fusarium argentea*, *Fusarium solani*, *Streptococcus spp.*, *Anthracis chinensis*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium oxysporum*, and *Cyclocarya pallida*.

[0026] In this invention, the plants preferably include traditional Chinese medicinal herbs and / or agricultural crops. The traditional Chinese medicinal herbs preferably include kudzu root, chrysanthemum, atractylodes macrocephala, peony root, scrophularia root, and ophiopogon japonicus. The agricultural crops preferably include at least one of the following: grain crops, oil crops, vegetables, fruit trees, medicinal herbs, flowers, sugar crops, and fiber crops. The grain crops preferably include corn, wheat, rice, and sorghum. The oil crops preferably include rapeseed, peanuts, and soybeans. The vegetables preferably include rapeseed, cucumber, tomato, eggplant, pepper, lettuce, and cabbage. The fruit trees preferably include bananas, grapes, and citrus fruits.

[0027] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008, the biocontrol agent, the culture of the *Aspergillus spp.* strain GGNSJ008, or the compound microbial fertilizer in kudzu cultivation or the control of kudzu diseases. The kudzu diseases include at least one of the following pathogenic fungi: *Fusarium racifolium*, *Fusarium solani*, *Streptococcus spp.* (the kudzu leaf spot pathogen), *Anthracis chinensis*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium oxysporum*, and *Cyclocarya pallida*.

[0028] This invention provides the application of the *Aspergillus spp.* strain GGNSJ008 and / or a culture of the *Aspergillus spp.* strain GGNSJ008 in the preparation of a broad-spectrum antibacterial agent. The broad-spectrum antibacterial agent targets at least one of the following pathogenic fungi: *Fusarium argentis*, *Fusarium solani*, *Streptococcus spp.*, *Anthracis ciliatus*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium verticillatum*, and *Cyclocarya pallida*.

[0029] This invention provides a method for preventing and controlling plant diseases, which involves applying the Aspergillus oryzae strain GGNSJ008, the biocontrol agent, a culture of the Aspergillus oryzae strain GGNSJ008, or the compound microbial fertilizer.

[0030] This invention does not impose any special restrictions on the species of plants, as described above. Since the *Aspergillus spp.* strain GGNSJ008 is an endophytic fungus in the rhizosphere of kudzu and has good parasitic ability within the roots, the application method preferably includes root irrigation to ensure the stable colonization of the *Aspergillus spp.* strain GGNSJ008 in the plant rhizosphere.

[0031] The following detailed description, in conjunction with embodiments, illustrates the Aspergillus oryzae strain GGNSJ008, which possesses resistance to kudzu root rot pathogens, and its applications. However, these descriptions should not be construed as limiting the scope of protection of this invention.

[0032] Example 1 Isolation and identification of endophytic fungi from kudzu root 1. Collection of kudzu root samples Kudzu samples were collected from kudzu bases in Jiangxi Province. Healthy, disease-free samples were selected, dug out intact, and the joints were rinsed with purified water and disinfected with 75% ethanol before being placed in sterile bags and stored in a low-temperature incubator. Six plants were collected from each base. After the samples were collected, they were immediately transported back to the laboratory and stored in a 4°C refrigerator.

[0033] 2. Culture medium preparation PDA medium: Take 39 g of powder (OXOID potato dextrose agar medium) and add 1 L of pure water. After sonication to dissolve, sterilize at 121 °C for 20 min. Prepare PDA solid plates in a clean bench for later use.

[0034] 3. Surface disinfection of kudzu root samples After absorbing moisture from the surface of the kudzu root sample with sterile filter paper, the epidermis of the kudzu root was removed with a sterile knife, and the internal tissue was extracted and cut into 5 cm × 5 cm tissue blocks. Each tissue block was sterilized with 75% ethanol for 10 s, then treated with 2% sodium hypochlorite solution for 5 min, rinsed 3 times with sterile water, air-dried, and placed on PDA plates for inverted incubation at 28 ℃.

[0035] 4. Purification and preservation of endophytic fungi Observe the colony situation on the plate. Once a single colony grows on the PDA plate, use a tool to pick the colony and place it on a new PDA medium for purification. After complete purification, preserve the strain by preparing mycelial cakes using a sterile punch. Then, add 1.5 mL of 30% sterile glycerol to a 2 mL cryovial, and add the mycelial cakes to the cryovial, 3-4 pieces per cryovial.

[0036] 5. Identification of Endophytic Fungi The morphological identification method is as follows: the strain is inoculated in the center of a PDA culture dish and cultured in an incubator at 28 ℃ for 7 days. During the growth of the strain, the characteristics of colony color, size, texture, surface, edge and other features are recorded. After sporulation, the morphology of hyphae and spores is observed under an optical microscope. Morphological identification is performed with reference to the "Handbook of Fungal Identification" (Shanghai Science and Technology Press, 1979).

[0037] The molecular biological identification method is as follows: The purified endophytic fungus rDNA-ITS gene was amplified using a fungal extraction direct amplification kit (Thermo Fisher Scientific). Fungal DNA was extracted with lysis buffer, and a 25 μL amplification system was prepared, consisting of 12 μL of 2×mix, 1 μL each of forward and reverse primers, 1 μL of DNA template, and ddH2O added to a final volume of 25 μL. Primers are listed in Table 1. Amplification conditions were: 98 ℃ for 5 min; 98 ℃ for 5 s, 58 ℃ for 5 s, 72 ℃ for 30 s, for 35 cycles; 72 ℃ for 1 min. After amplification, the sample was sent to BGI Genomics for bidirectional sequencing to complete preliminary identification.

[0038] Table 1 Primer sequences involved in this embodiment

[0039] 6. Identification results of endophytic fungus GGNSJ008 Morphological identification results such as Figure 1 As shown. Colonies cultured on PDA medium for 7 days reached a diameter of 2.3 cm, were irregularly round, velvety grayish-white, wrinkled, with a yellowish-brown back. The conidial heads were densely spherical or cylindrical, the conidiophores were 4-9 µm in diameter, the vesicles were flask-shaped with a diameter of 10-20 µm, and the conidial spheroids had slightly rough walls with a diameter of 2.0-3.5 µm.

[0040] Molecular biological identification results showed that this strain... rDNA-ITS、RPB2 , CAL , TUB The lengths of the gene sequences are 603bp (SEQ ID NO:1), 1221bp (SEQ ID NO:2), 772bp (SEQ ID NO:3), and 545bp (SEQ ID NO:4), respectively.

[0041] After BLAST comparison, the above four sequences of related Aspergillus fungi were downloaded. The four sequences of each strain were tandemly compared, and a phylogenetic tree was constructed using the neighbor-joining method.

[0042] Figure 2 The results showed that GGNSJ008 was associated with Aspergillus oryzae (… Aspergillus nivusThe fungi clustered into one clade, while other Aspergillus species formed independent clades. Based on sequence similarity and phylogenetic relationships, strain GGNSJ008 was identified as... Aspergillus snowy The strain GGNSJ008 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on April 8, 2026, with accession number CGMCC No. 42606.

[0043] Example 2 Detection of antifungal function of endophytic fungal strain GGNSJ008 from kudzu root 1. Plate confrontation experiment between endophytic fungi and common pathogens of kudzu. Ten pathogenic fungi were tested, including Fusarium argentis, the pathogen of kudzu root rot. Fusarium acuminata Fusarium solani () Fusarium solani ), the pathogen of kudzu leaf spot, *Streptococcus parviflora* ( Alternaria alternata ), and other common plant pathogenic fungi: fruit anthracnose ( Colletotrichum fructicola ), Sclerotinia sclerotiorum ( Sclerotinia sclerotia ), Karst anthrax bacteria ( Colletotrichum karsti Fusarium graminearum ( ), Fusarium grasses Fusarium oxysporum ( Fusarium oxysporum ), Fusarium verticillata ( Fusarium whorl-shaped ), multi-master spores ( Corynespora cassicola Using a sterile punch, 0.5 mm diameter mycelial cakes of the aforementioned plant pathogens were taken from each PDA medium and placed at 1 / 4 of the diameter. Endophytic fungal strain GGNSJ008 was symmetrically inoculated into each medium to create an antagonistic relationship, serving as the experimental group. PDA medium without inoculation of the antagonistic fungal strain served as the control group. Each treatment was repeated three times. The inhibition rate was calculated using the following formula.

[0044] Inhibition rate (%) = (colon radius of control group - colony radius of treatment group) / colony radius of control group × 100%.

[0045] 2. Results of antibacterial activity assay for endophytic fungal strain GGNSJ008 The antifungal effect of GGNSJ008 against 10 common pathogenic fungi was detected using the plate confrontation method. Figure 3 And Table 2.

[0046] The results showed that GGNSJ008 had the most significant antibacterial effect against Fusarium argentis, with an inhibition rate of 31.76%. Figure 3In the first category, *Fusarium graminearum* (a) was the most effective inhibitor, followed by *Fusarium graminearum*, *Fusarium verticillatum*, and *Anthracnose karstii*, with inhibition rates of 22.22%, 20.94%, and 20%, respectively. Furthermore, it also exhibited varying degrees of inhibitory activity against *Fusarium oxysporum*, *Fusarium solani*, *Sclerotinia sclerotiorum*, *Cyclocarya paliurus*, *Anthracnose spp.*, and *Alternaria alternata*. Therefore, strain GGNSJ008, derived from endophytic fungi of kudzu root, demonstrates excellent broad-spectrum inhibitory effects against the aforementioned 10 kudzu root pathogens, and plays a crucial role in controlling root rot, leaf spot, anthracnose, sclerotinia stem rot, soft rot, wheat scab, wilt, ear rot, and *Cyclocarya paliurus* leaf spot in various plants, including kudzu root.

[0047] Table 2. Statistics on the antifungal inhibition rate of GGNSJ008 against antagonistic pathogens.

[0048] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A type of snow-white Aspergillus ( Aspergillus niveus The strain GGNSJ008 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42606.

2. A biocontrol agent, characterized in that, Includes the Aspergillus oryzae strain GGNSJ008 of claim 1 or a culture of the Aspergillus oryzae strain GGNSJ008.

3. A compound microbial fertilizer, characterized in that, Includes the Aspergillus oryzae strain GGNSJ008 of claim 1, the biocontrol agent of claim 2, or the culture of the Aspergillus oryzae strain GGNSJ008 and biofertilizer; The mass ratio of the Aspergillus oryzae strain GGNSJ008, the biocontrol agent, or the culture of the Aspergillus oryzae strain GGNSJ008 to the biofertilizer is 1~5:1~20.

4. The compound microbial fertilizer according to claim 3, characterized in that, The bio-fertilizer includes at least one of the following: well-rotted poultry and livestock manure organic fertilizer, well-rotted agricultural waste organic fertilizer, and well-rotted medicinal residue or fungal residue organic fertilizer.

5. The application of the *Aspergillus oryzae* strain GGNSJ008 of claim 1, the biocontrol agent of claim 2, the culture of the *Aspergillus oryzae* strain GGNSJ008, or the compound microbial fertilizer of claim 3 or 4 in the control of plant diseases caused by at least one of the following pathogenic fungi: *Fusarium argenteum* (… Fusarium acuminatum Fusarium solani () Fusarium solani ), the pathogen of kudzu leaf spot, *Streptococcus parviflora* ( Alternaria alternata Fruit anthrax ( Colletotrichum fructicola ), Sclerotinia sclerotiorum ( Sclerotinia sclerotiorum ), Karst anthrax bacteria ( Colletotrichum karsti Fusarium graminearum ( ), Fusarium graminearum Fusarium oxysporum ( Fusarium oxysporum ), Fusarium verticillata ( Fusarium verticillioides ) and multi-master spirochete ( Corynespora cassiicola ).

6. The application according to claim 5, characterized in that, The plants mentioned include traditional Chinese medicine and / or crops.

7. The application of the Aspergillus oryzae strain GGNSJ008 of claim 1, the biocontrol agent of claim 2, the culture of the Aspergillus oryzae strain GGNSJ008, or the compound microbial fertilizer of claim 3 or 4 in the cultivation of kudzu or the control of kudzu diseases, wherein the kudzu diseases include diseases caused by at least one of the following pathogenic fungi: Fusarium racifolium, Fusarium solani, the kudzu leaf spot pathogen Lepidoptera, Anthracnose sclerotiorum, Sclerotium sclerotiorum, Anthracnose karstii, Fusarium graminearum, Fusarium oxysporum, Fusarium verticillata, and Ligustrum multiflorum.

8. The use of the Aspergillus oryzae strain GGNSJ008 of claim 1 and / or the culture of the Aspergillus oryzae strain GGNSJ008 in the preparation of a broad-spectrum antibacterial agent, wherein the broad-spectrum antibacterial agent targets at least one of the following pathogenic fungi: Fusarium argentis, Fusarium solani, *Streptococcus spp.*, *Anthracis ciliatus*, *Sclerotium sclerotiorum*, *Anthracis karstii*, *Fusarium graminearum*, *Fusarium oxysporum*, *Fusarium verticillatum*, and *Cyclocarya pallida*.

9. A method for preventing and controlling plant diseases, characterized in that, Apply the Aspergillus oryzae strain GGNSJ008 of claim 1, the biocontrol agent of claim 2, the culture of the Aspergillus oryzae strain GGNSJ008, or the compound microbial fertilizer of claim 3 or 4.

10. The method according to claim 9, characterized in that, The application method includes root irrigation.

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