A raspberry compound beverage with intelligence and nerve protection effect and its preparation method and application

Abstract

This invention belongs to the field of functional food and health product technology, specifically relating to a raspberry complex beverage with cognitive-enhancing and neuroprotective effects, its preparation method, and its application. The beverage is a compound of various natural ingredients, including raspberry, peony flower, longan pulp, and jujube seed, in a specific ratio. The preparation method includes extraction, concentration, blending, and sterilization. Cell and animal experiments have confirmed that this beverage significantly improves cognitive function and reduces oxidative stress and inflammation levels in the brain through a synergistic effect of anti-Aβ, antioxidant, and anti-inflammatory properties, with high bioavailability and good safety. This invention is particularly suitable for preparing various products for the prevention, treatment, adjunctive treatment, or improvement of Alzheimer's disease, improvement of cognitive impairment, and relief of brain aging and neuroinflammation.

Description

Technical Field

[0001] This invention belongs to the field of functional food and health product technology, specifically relating to a raspberry complex beverage with brain-boosting and neuroprotective effects, its preparation method and application, and is particularly suitable for preparing various products with the functions of preventing, treating and adjuvant treatment of Alzheimer's disease, improving cognitive impairment, and alleviating brain aging and neuroinflammation. Background Technology

[0002] Alzheimer's disease (AD) is currently the most prevalent neurodegenerative disease worldwide. With the accelerating aging of the global population, the number of AD patients is increasing year by year, placing a heavy burden on social healthcare systems and patients' families. The core pathological features of AD have been widely agreed upon by the academic community, mainly including the abnormal deposition of β-amyloid protein (Aβ) in the hippocampus and cortex of the brain to form senile plaques, the hyperphosphorylation and aggregation of Tau protein to form neurofibrillary tangles, accompanied by continuous oxidative stress damage and chronic neuroinflammatory response in the brain. The combined effect of multiple pathological mechanisms leads to progressive neuronal death, ultimately causing irreversible cognitive decline.

[0003] Currently, the types of drugs available for the clinical treatment of Alzheimer's disease (AD) are limited. Mainstream drugs, such as cholinesterase inhibitors, primarily aim to alleviate clinical symptoms, only slowing the rate of cognitive decline to a certain extent. They cannot reverse the disease progression or pathologically halt AD progression. Furthermore, long-term use of these chemical drugs carries risks of gastrointestinal adverse reactions, liver and kidney damage, and other side effects, limiting drug safety and patient compliance. Therefore, developing functional foods or adjunctive therapies with natural sources and low side effects that can prevent or delay AD progression at the pathological level through multi-target synergistic effects—simultaneously achieving anti-Aβ neurotoxicity, scavenging free radicals, and inhibiting chronic neuroinflammation—has become a research hotspot in the field of AD prevention, possessing significant social and industrial value.

[0004] Among natural plant raw materials, raspberries, as a common fruit used in both medicine and food, are rich in various polyphenolic active ingredients such as proanthocyanidins, gallic acid, and ellagic acid. Existing basic research has confirmed that its extracts have clear in vitro antioxidant activity and in vivo anti-inflammatory effects, which can reduce the level of peripheral inflammatory factors and reduce oxidative damage in the brain, thus possessing the material basis for intervening in the pathological process of Alzheimer's disease. Fragrant peony flowers are a new food raw material approved in recent years. They are fresh and dried flower buds of cultivated peony varieties belonging to the Paeoniaceae family. The production source is stable, and the safety of consumption has been recognized by toxicological assessment. They can be used in accordance with regulations in the production of various ordinary foods. Peony flowers contain various highly active flavonoids such as luteolin, apigenin, and quercetin. Traditional Chinese medicine records the use of peony flower tea in dietary therapy, stating that it "calms the mind, improves intelligence, and invigorates the spleen." Modern in vitro antioxidant experiments have further confirmed that the DPPH free radical scavenging capacity of total flavonoids from peony flowers is close to that of vitamin C at the same concentration, and its antioxidant activity is significantly superior to that of most common edible flowers such as chrysanthemum, rose, and calendula, making it a natural antioxidant raw material with great development potential. Currently, there are no research reports in the field of combining food ingredients such as raspberries with peony flowers for anti-AD cognitive improvement, and the synergistic neuroprotective effect of the two has not been publicly verified. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the purpose of this invention is to provide a raspberry complex beverage with clear cognitive-enhancing and neuroprotective effects, as well as its preparation method and applications.

[0006] Specifically, the present invention is achieved through the following technical solutions: In a first aspect, the present invention provides a raspberry complex beverage with cognitive and neuroprotective effects, the beverage being made from the following ingredients in parts by weight: 5-15 parts raspberry, 2-15 parts peony flower, 2-15 parts raisins, 5-20 parts longan pulp, 1-10 parts poria cocos, 5-15 parts wolfberry, 1-10 parts jujube seed, 5-20 parts jujube, and 1-10 parts walnut kernel.

[0007] Alternatively, in the above-mentioned raspberry complex beverage, the beverage is made from the following raw materials in parts by weight: 8-12 parts raspberry, 6-10 parts peony flower, 6-10 parts raisins, 10-15 parts longan pulp, 3-7 parts poria cocos, 8-12 parts wolfberry, 3-7 parts jujube seed, 10-15 parts jujube, and 3-6 parts walnut kernel.

[0008] Alternatively, in the above-mentioned raspberry complex beverage, the beverage is made from the following ingredients in parts by weight: 10 parts raspberry, 8 parts peony flower, 8 parts raisins, 12 parts longan pulp, 5 parts poria cocos, 10 parts wolfberry, 5 parts jujube seed, 12 parts jujube, and 4 parts walnut kernel.

[0009] In a second aspect, the present invention provides a method for preparing the raspberry complex beverage described in the first aspect above, the method comprising the following steps: Step (1): Mix and crush the raw materials; Step (2): Heat and extract with water, filter, and obtain the extract; Step (3): Concentrate, flavor, and adjust the volume of the extract; Step (4): Sterilization and filling.

[0010] As an optional method, in the above preparation method, the heating extraction temperature in step (2) is 85-100℃, the extraction time is 1.5-2.5 hours, and the extraction is repeated 1-2 times.

[0011] As an optional approach, in the above preparation method, the flavoring agent added in step (3) is honey, xylitol, steviol glycosides or mogrosides.

[0012] As an optional approach, in the above preparation method, step (4) uses high-temperature instantaneous sterilization or ultra-high-temperature instantaneous sterilization.

[0013] Preferably, the preparation method includes the following steps: (1) Raw material pretreatment: Weigh each raw material according to the above proportions, wash and crush to a particle size ≤ 5 mm; (2) Extraction: Place the mixed raw materials in an extraction tank, add 8-12 times (preferably 10 times) of the total mass of the raw materials in purified water, and extract in a water bath at 85-100℃ (preferably 95℃) for 1.5-2.5 hours (preferably 2 hours), filter, and collect the filtrate; add 8-10 times (preferably 8 times) of the mass of purified water to the filter residue again, and repeat the extraction once under the same conditions, combine the two extracts, and filter with a 200-mesh filter cloth; (3) Volume adjustment and preparation: Concentrate the combined filtrate under reduced pressure at 60°C to 1 / 3-1 / 2 of the original volume (preferably 40%), add 0.5-1.5% of flavoring agent (such as honey or xylitol) by mass of the concentrate, and add purified water to adjust the volume to the designed capacity. (4) Sterilization and filling: High temperature instantaneous sterilization (121℃, 15-20 minutes) or ultra-high temperature instantaneous sterilization (UHT, 135℃, 5 seconds) is used. After cooling to below 25℃, the product is filled and sealed in a sterile environment.

[0014] In a third aspect, the present invention provides the use of the raspberry complex beverage described in the first aspect above, or the raspberry complex beverage prepared by the preparation method described in the second aspect above, in the preparation of products having the effects of preventing, treating, adjuvant treating or improving Alzheimer's disease, improving cognitive impairment, alleviating brain aging and neuroinflammation.

[0015] Alternatively, in the above applications, the product may be a functional food, health product, or medicine.

[0016] Compared with the prior art, the present invention has the following beneficial effects: (1) This invention is the first to scientifically combine raspberry with peony flower, and together with raisins, longan pulp and other raw materials, to construct a triple synergistic network of anti-Aβ-antioxidant-anti-inflammatory, and to intervene from the core pathological link of AD.

[0017] (2) Through in vitro and in vivo experiments, this invention has confirmed that the fragrant peony flower is an irreplaceable core ingredient for the beverage of this invention to exert its brain-boosting and neuroprotective effects.

[0018] (3) All raw materials used in this invention are food and medicine homologous substances, and have high safety. Compared with traditional solid dosage forms, liquid dosage forms are easier for elderly people to swallow and absorb, and have high bioavailability.

[0019] (4) The present invention adopts conventional water extraction and sterilization processes, which are simple, cost controllable, and easy to achieve standardized and large-scale production. Attached Figure Description

[0020] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings: Figure 1 In vitro neuroprotective effects of raspberry complex beverage on HT-22 cells. A: Safety screening of different concentrations of beverage (cell viability, MTT assay); B: Anti-Aβ effects. 1-42 Cell survival rate after induced damage. Compared with the normal control group, ### P <0.001; compared with the model group, P <0.05, P <0.01, P <0.001.

[0021] Figure 2 Results of the Y-maze experiment. A: Total number of arm entries; B: Spontaneous alternation response rate. Compared with the normal control group, ### P <0.001; compared with the model group, P <0.01, P <0.001.

[0022] Figure 3 Results of the Morris water maze experiment. AD: latency period for navigation; EF: space exploration. Compared with the normal control group, ## P <0.01, ### P <0.001; compared with the model group, P <0.05, P <0.01, P <0.001.

[0023] Figure 4 AChE activity in hippocampal tissue. Compared with the normal control group, ### P <0.001; compared with the model group, P <0.01, P <0.001.

[0024] Figure 5 MDA content in hippocampal tissue. Compared with the normal control group, ### P <0.001; compared with the model group, P <0.05, P <0.001.

[0025] Figure 6 SOD activity in hippocampal tissue. Compared with the normal control group, ### P <0.001; compared with the model group, P <0.01, P <0.001.

[0026] Figure 7 : Hippocampal neuroinflammatory factors. A: TNF-α level; B: IL-6 level. Compared with the normal control group, ### P<0.001; compared with the model group, P <0.01, P <0.001. Detailed Implementation

[0027] The present invention will be further described below with reference to specific embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the invention.

[0028] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased through legitimate channels.

[0029] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods. Unless otherwise specified, the experimental materials used in the following embodiments are commercially available products.

[0030] Unless otherwise stated, all percentages and parts in this invention refer to weight percentages and weight parts.

[0031] Preparation Example: Example 1: A raspberry complex beverage with brain-boosting and neuroprotective effects, the beverage being made from the following ingredients: 100 g raspberries, 80 g peony flowers, 80 g raisins, 120 g longan pulp, 50 g poria cocos, 100 g goji berries, 50 g jujube seeds, 120 g jujubes, and 40 g walnut kernels.

[0032] The preparation method of the raspberry complex beverage includes the following steps: Step (1): After cleaning the raw materials, mix them and crush them to a particle size ≤ 5 mm; Step (2): Add 10 times the total mass of the raw materials to purified water, extract in a 95℃ water bath for 2 hours, filter to obtain filtrate, add 8 times the amount of water to the residue and extract in the same way for 2 hours, combine the two extracts and filter with a 200-mesh filter cloth; Step (3): Concentrate the combined filtrate under reduced pressure at 60°C to 40% of the original volume, add 1% mogroside by weight of the concentrate, and add purified water to make up to 1 L; Step (4): The raspberry complex beverage is obtained by sterilization at UHT 135℃ for 5 seconds, cooling, and aseptic filling.

[0033] Comparative Example 1: A beverage made from the following ingredients: 80 g of fragrant peony flowers. The preparation method of the beverage is the same as in Example 1.

[0034] Comparative Example 2: A beverage made from the following ingredients: 100 g raspberries, 80 g raisins, 120 g longan pulp, 50 g Poria cocos, 100 g wolfberries, 50 g jujube seeds, 120 g jujubes, and 40 g walnut kernels. The preparation method of the beverage is the same as in Example 1.

[0035] Example of effect: Example 1: Cellular level anti-AD experiment (anti-Aβ) This embodiment aims to verify the effects of raspberry complex beverage on β-amyloid protein (Aβ1), a core pathological factor in Alzheimer's disease. 42 Protective effect against neurotoxicity caused by ).

[0036] 1. Cell culture: The mouse hippocampal neuronal cell line HT-22 was selected. Cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin antibiotics at 37°C in a 5% CO2 incubator. Cells in the logarithmic growth phase were used in the experiment.

[0037] 2. Drug safety screening: HT-22 cells were seeded in 96-well plates (1×10⁶ cells / well). 4 (samples / well), adhered to the culture vessel for 24 hours. Discard the old solution and add culture medium containing different concentrations of the test sample (based on the raspberry complex beverage prepared in Example 1, converted to raspberry extract concentrations: 12.5, 25, 50, 100, 200, 400, 800 µg / mL). After incubation for 24 hours, add MTT solution and continue incubation for 4 hours. Discard the supernatant, add 150 µL DMSO to dissolve and crystallize, and measure the absorbance (OD value) at 570 nm using a microplate reader.

[0038] 3. Anti-Aβ nerve injury experiment (1) Modeling agent: Aβ1 42 The lyophilized powder was dissolved in DMSO, then diluted with PBS, and incubated at 37°C for 3-7 days to form a neurotoxic oligomer.

[0039] (2) Group processing: Blank control group (Control): Normal culture medium.

[0040] Model group: Add Aβ1 42Oligomers (final concentration 20 μM), incubated for 24 h.

[0041] Positive control group: donepezil (final concentration 10 μM).

[0042] Beverage administration group: First, different concentrations of the test sample (based on the raspberry compound beverage prepared in Example 1, converted to raspberry extract concentrations: 50, 100, 200 μg / mL) were added and pre-incubated for 2 h, followed by the addition of Aβ1. 42 Incubate for a total of 24 hours.

[0043] (3) Detection: Cell viability was determined by the MTT assay.

[0044] 4. Experimental Results 4.1 Cytotoxicity Results like Figure 1 As shown in Figure A, the survival rate of HT-22 cells remained above 93% within the concentration range of 12.5-200 µg / mL, with no statistical difference compared to the control group, indicating that the beverage has no neurotoxicity at effective doses.

[0045] 4.2 Results of resistance to Aβ damage like Figure 1 As shown in B, the model group cells were treated with Aβ1. 42 After oligomer treatment, the cell survival rate significantly decreased to approximately 54%, indicating the successful establishment of the AD cell model. The raspberry complex beverage dose-dependently improved cell survival, with the high-dose group (200 µg / mL) recovering a survival rate of 86.5%, demonstrating a significant protective effect approaching that of the positive control drug donepezil. This indicates that the complex beverage can effectively antagonize Aβ-induced neuronal death.

[0046] Example 2: Animal-level anti-AD experiment (Aβ1) 42 Hippocampal injection model) This embodiment uses the classic Aβ stereotactic injection model to directly simulate oxidative stress, inflammatory outbreaks, and memory loss caused by plaque deposition in the brain of AD patients, thus verifying the in vivo efficacy of the beverage of this invention.

[0047] 1. Laboratory animals and grouping (1) Animals: C57BL / 6 male mice, 8 weeks old, n=12 / group.

[0048] (2) Grouping: Sham surgery group (blank): hippocampal injection of saline solution + gavage.

[0049] AD model group (model): Hippocampal injection of Aβ1 42 +Gastric enema.

[0050] Positive control group (positive): hippocampal injection of Aβ1 42 + Donepezil (3 mg / kg) administered by gavage.

[0051] Comparative Example 1 (Control 1): Hippocampal injection of Aβ1 42 + Gavage 10 mL / kg of the beverage stock solution prepared in Comparative Example 1.

[0052] Comparative Example 2 (Control 2): ​​Hippocampal injection of Aβ1 42 +Administer 10 mL / kg of the beverage concentrate prepared in Comparative Example 2 via gavage.

[0053] Example 1 (Implementation 1): Hippocampal injection of Aβ1 42 +The beverage stock solution prepared in Example 1 by gavage.

[0054] 2. Modeling and Dosing Regimen Modeling surgery: Mice were anesthetized and fixed in a stereotaxic apparatus. Aβ1 was slowly injected into the bilateral CA1 region of the hippocampus (coordinates: 2.0 mm posterior to the anterior fontanelle, 1.5 mm lateral, 2.0 mm deep). 42 Oligomers (2 μL per side). The sham-operated group received an equal volume of normal saline.

[0055] Administration cycle: Start daily gavage administration 3 days after surgery for 28 consecutive days.

[0056] 3. Behavioral tests Y-maze experiment: The apparatus consists of three equal-length arms (30 cm × 8 cm × 15 cm) with an included angle of 120°, labeled A, B, and C. Mice are placed facing the center and allowed to explore freely for 8 minutes. The order in which the mice enter each arm is recorded (all four limbs must be fully entered for the experiment to be valid). After the experiment, the total number of arm entries and spontaneous alternation behaviors of the mice are counted.

[0057] Morris Water Maze: The experimental setup consisted of a black circular pool with a diameter of 120 cm and a height of 50 cm. The water temperature was kept constant at 22 ± 1℃, and food-grade titanium dioxide was added to the water to make the platform invisible. The pool was divided into four quadrants, with the platform (10 cm in diameter) fixed in the center of the third quadrant (target quadrant), submerged 1 cm underwater. The experiment consisted of two phases: navigation and spatial exploration. The first phase was the navigation test (lasting 4 days): training was conducted 4 times a day. Mice were placed in the water from the midpoint of the wall in each of the four quadrants, facing the pool wall, and the time it took for the mouse to find the hidden platform was recorded (escape latency). If the mouse did not find the platform within 60 seconds, it was guided onto the platform and remained there for 15 seconds; the latency was recorded as 60 seconds. The second phase was the spatial exploration test (day 5): the platform was removed, and the mouse was placed in the water from the midpoint of the first quadrant opposite the target quadrant, facing the pool wall, and its swimming trajectory was recorded over 60 seconds. The main detection indicators include the time the mouse spends in the target quadrant (the quadrant where the original platform is located) and the number of times it crosses the original platform location, in order to evaluate the mouse's long-term spatial learning and memory ability.

[0058] 4. Brain tissue biochemical testing The day after the behavioral tests, mice were euthanized by decapitation, and bilateral hippocampal tissue was rapidly dissected and isolated on an ice tray. After accurate weighing, pre-chilled PBS was added at a weight (g):volume (mL) ratio of 1:9, and the mixture was mechanically homogenized in an ice-water bath to prepare a 10% tissue homogenate. The homogenate was centrifuged at 4°C and 3000-4000 rpm for 10-15 minutes, and the supernatant was carefully collected as the test sample. The total protein concentration of each sample was determined using a BCA protein quantification kit for standardization correction.

[0059] For oxidative stress detection, the kit instructions were strictly followed, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected by chemical colorimetry.

[0060] In terms of neuroinflammation detection, enzyme-linked immunosorbent assay (ELISA) was used to detect the content of TNF-α and IL-6 in hippocampal tissue. The sample was added to a microplate pre-coated with antibody and incubated. After washing the plate, biotin-labeled antibody and HRP conjugate were added for color development. The optical density (OD) value was measured and the concentration was calculated.

[0061] In terms of cholinergic system detection, the activity of acetylcholinesterase (AChE) was determined by chemical colorimetry.

[0062] 5. Experimental Results 5.1 Y-maze test like Figure 2 A and Figure 2As shown in Figure B, behavioral test results indicated that the total number of arm insertions by mice in each group within 8 minutes was between 30 and 35, with no statistically significant difference between groups. This result suggests that Aβ1... 42 Hippocampal injection and subsequent drug intervention did not significantly affect the spontaneous activity and exploratory desire of mice, thus ruling out the potential interference of motor dysfunction on working memory evaluation. Regarding the spontaneous alternation response rate, the model group mice showed a significantly reduced rate, approaching the level of random exploration, indicating severe impairment of short-term working memory. The raspberry complex beverage intervention significantly improved this deficiency, with increased alternation rates in Comparative Example 2 and Example 1, and Example 1 recovering to levels close to the sham-operated group. Furthermore, these results also show that although *Peony tataricus* flower alone (Comparative Example 1) had virtually no effect on the above indicators, its addition to the raspberry complex beverage of this invention produced a significant synergistic effect on improving short-term memory in mice.

[0063] 5.2 Morris Water Maze Test like Figure 3 A to Figure 3 As shown in Figure F, on day 4 of the navigation training, the latency of the model group mice in finding the concealed platform was still significantly longer than that of the sham-operated group. The latency of the raspberry complex beverage group mice was significantly shortened, and the latency of the Example 1 group was comparable to that of the positive control group, indicating that the beverage significantly improved Aβ1. 42 Induced spatial learning impairment. After the platform was removed, the model group mice traversed the original platform location fewer times and spent less time in the target quadrant, significantly lower than the sham-operated group, indicating spatial memory deficit. The raspberry complex beverage intervention group showed a significant increase in the number of traversals, and the time spent in the target quadrant was significantly prolonged in both Comparative Example 2 and Example 1 groups, confirming that the raspberry complex beverage of this invention can enhance mice's precise spatial location and long-term memory. Furthermore, these results also show that although *Peony tataricus* flower alone (Comparative Example 1) had virtually no effect on the above indicators, its addition to the raspberry complex beverage of this invention produced a significant synergistic effect on improving mice's precise spatial location and long-term memory.

[0064] 5.3 AChE activity like Figure 4 As shown, AChE activity was abnormally elevated in the hippocampus of the model group, leading to excessive breakdown of neurotransmitters. The raspberry complex beverage of this invention significantly inhibited AChE activity; this protective effect on the cholinergic system is one of the key mechanisms by which it improves memory.

[0065] 5.4 MDA content in hippocampal tissue like Figure 5 As shown, the MDA content in the hippocampus of the model group surged, suggesting that Aβ injection induced severe lipid peroxidation. The MDA level in the raspberry complex beverage treatment group of this invention decreased in a dose-dependent manner, demonstrating that the complex beverage of this invention has a potent antioxidant capacity in the brain.

[0066] 5.5 Hippocampal SOD activity like Figure 6 As shown, the activity of the antioxidant enzyme SOD in the hippocampus of mice was detected using a chemical colorimetric method. The results showed that SOD activity in the hippocampus of the model group mice was significantly reduced, indicating that Aβ injection caused severe damage to the brain's antioxidant defense system. After intervention with the raspberry complex beverage of this invention, SOD activity showed a significant recovery. This confirms that the complex beverage of this invention can effectively enhance the brain's antioxidant capacity, scavenge free radicals, and thus protect neurons from oxidative damage.

[0067] 5.6 Hippocampal neuroinflammatory factors like Figure 7 A to Figure 7 As shown in Figure B, the ELISA results revealed significantly higher levels of pro-inflammatory factors TNF-α and IL-6 in the hippocampus of the model group mice compared to the sham-operated group, indicating significant neuroinflammation in the brain. The raspberry complex beverage of this invention effectively inhibited the inflammatory response, significantly reducing TNF-α levels, which helps reduce inflammation-mediated neuronal damage. Similarly, IL-6 levels also significantly decreased after intervention with the raspberry complex beverage of this invention. This demonstrates that the complex beverage of this invention can effectively inhibit Aβ-induced neuroinflammatory responses.

[0068] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A raspberry complex beverage with brain-boosting and neuroprotective effects, characterized in that, The beverage is made from the following ingredients in parts by weight: 5-15 parts raspberry, 2-15 parts fragrant peony flower, 2-15 parts raisins, 5-20 parts longan pulp, 1-10 parts poria cocos, 5-15 parts wolfberry, 1-10 parts jujube seed, 5-20 parts jujube, and 1-10 parts walnut kernel.

2. The raspberry complex beverage according to claim 1, characterized in that, The beverage is made from the following ingredients in parts by weight: 8-12 parts raspberry, 6-10 parts peony flower, 6-10 parts raisins, 10-15 parts longan pulp, 3-7 parts poria cocos, 8-12 parts goji berries, 3-7 parts jujube seed, 10-15 parts jujube, and 3-6 parts walnut kernel.

3. The raspberry complex beverage according to claim 2, characterized in that, The beverage is made from the following ingredients in parts by weight: 10 parts raspberry, 8 parts fragrant peony flower, 8 parts raisins, 12 parts longan pulp, 5 parts poria cocos, 10 parts wolfberry, 5 parts jujube seed, 12 parts jujube, and 4 parts walnut kernel.

4. The method for preparing the raspberry complex beverage according to any one of claims 1 to 3, characterized in that, The preparation method includes the following steps: Step (1): Mix and crush the raw materials; Step (2): Heat and extract with water, filter, and obtain the extract; Step (3): Concentrate, flavor, and adjust the volume of the extract; Step (4): Sterilization and filling.

5. The preparation method according to claim 4, characterized in that, The temperature for heating and extraction in step (2) is 85-100℃, the extraction time is 1.5-2.5 hours, and the extraction is repeated 1-2 times.

6. The preparation method according to claim 4, characterized in that, The flavoring agent added in step (3) is honey, xylitol, steviol glycosides or mogrosides.

7. The preparation method according to claim 4, characterized in that, In step (4), high-temperature instantaneous sterilization or ultra-high-temperature instantaneous sterilization is used.

8. The use of the raspberry complex beverage according to any one of claims 1 to 3 or the raspberry complex beverage prepared by the preparation method according to any one of claims 4 to 7 in the preparation of products having the effects of preventing, treating, adjuvant treating or improving Alzheimer's disease, improving cognitive impairment, alleviating brain aging and neuroinflammation.

9. The application according to claim 8, characterized in that: The product is a functional food, health product, or medicine.

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