A male oil control component, preparation method and application thereof

Abstract

The present application relates to a male oil control component and its preparation method and application, the male oil control component of the present application is pseudomonas alternanivorans / norway spruce leaf fermentation product, the oil control component of the present application realizes the multilevel intervention to androgen-sebaceous gland axis, fundamentally reduces the problem of excessive skin oiling caused by androgen flourishing, and is not limited to the level of surface adsorption or single enzyme inhibition.In addition, the pseudomonas alternanivorans / norway spruce leaf fermentation product is a natural fermentation source, and the component is mild and safe.The active ingredient is prepared by the co-fermentation process of pseudomonas alternanivorans and norway spruce leaves of marine origin, and no chemical synthesis auxiliary agent and irritating solvent is used in the whole process, and the obtained product is tested to have no skin irritation, so that sensitive skin groups can use it safely.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to an oil-controlling ingredient specially developed for men, its preparation method, and its application. Background Technology

[0002] Men's facial skin secretes approximately twice as much sebum as women's. This difference is fundamentally due to the powerful regulation of sebaceous glands by androgens: testosterone in the body is converted into the more potent dihydrotestosterone (DHT) within sebaceous gland cells by type I 5α-reductase. DHT binds to the androgen receptor (AR) to form a transcription complex, which in turn upregulates the expression of lipid synthesis-related genes, driving sebaceous gland enlargement and continuous excessive sebum secretion. Therefore, the core target for oil control in men should focus on the 5α-reductase and androgen receptor pathways, rather than simply relying on surface cleansing or physical adsorption.

[0003] Currently, industry solutions for oil control in men mainly include physical adsorption (such as kaolin and silica powder), chemical exfoliation (such as salicylic acid), and indirect regulation of sebum secretion through ingredients such as niacinamide and zinc salts. Some products attempt to use plant extracts (such as saw palmetto) to inhibit 5α-reductase, but these solutions have significant limitations: First, most plant extracts primarily inhibit type II 5α-reductase (mainly found in the prostate), while their inhibitory efficacy against type I 5α-reductase, which plays a dominant role in the skin, is insufficient; second, existing technologies generally only focus on the single target of 5α-reductase, neglecting the fact that the overactivation of androgen receptors is also a key driver of sebum secretion. In summary, there is an urgent need to develop a men-specific oil-control active ingredient that can act on both 5α-reductase and androgen receptors while possessing good compatibility and safety. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide an oil-controlling ingredient specifically for men, its preparation method, and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a method for preparing an oil-controlling ingredient specifically formulated for men, comprising the following steps: S1. The *Pseudomonas aeruginosa* bacterial culture and Norway spruce leaf extract were mixed and subjected to aerobic fermentation to obtain the fermentation product; wherein the inoculum amount of *Pseudomonas aeruginosa* was 1-5% of the mass of the Norway spruce leaf extract. S2. The fermentation product obtained in step S1 is extracted by ultrasonication, centrifuged, the supernatant is collected and dried to obtain the Pseudomonas alterniflora / Norwegian spruce leaf fermentation product, which is the oil-controlling ingredient specially developed for men.

[0006] Preferably, in step S1, the aerobic fermentation temperature is 18-26℃, the dissolved oxygen (DO) level is 15-40%, and the time is 16-48 hours. It is important to note that the production of extracellular enzymes by bacteria requires oxygen, which significantly affects the DO value. When DO < 15%, localized anaerobic conditions occur, leading to decreased extracellular enzyme activity and ultimately poor oil-controlling activity; when DO > 40%, the polyphenols and other active substances in the Norway spruce leaf extract will be oxidized, resulting in poor oil-controlling effect.

[0007] Preferably, the preparation method of the *Pseudomonas alterniflora* bacterial suspension includes the following steps: inoculating *Pseudomonas alterniflora* into a marine culture medium for aerobic fermentation until the OD600 value reaches 0.6-1.5, thereby obtaining the *Pseudomonas alterniflora* bacterial suspension; wherein the aerobic fermentation temperature is 18-26℃ and the time is 18-36 hours. It should be noted that when the OD600 value is 1.0-1.2, the bacteria are in the logarithmic growth phase, with vigorous secretion of extracellular enzymes, which can effectively convert the substrates in the Norway spruce leaf extract. An OD value <0.6 indicates that the bacterial concentration is too low, resulting in insufficient extracellular enzyme secretion and ultimately poor oil control; an OD value >1.5 indicates that the bacteria have entered the stationary phase, with the appearance of dead cell debris, and the released intracellular proteases / phospholipases may degrade the required active molecules and potentially promote inflammation, making it unsuitable for sensitive skin.

[0008] Preferably, the pseudoalternating monoclonal bacteria is pseudoalternating monoclonal bacteria ATCC 19648 and / or pseudoalternating monoclonal bacteria ATCCBAA-376.

[0009] Preferably, in step S2, the ultrasonic extraction temperature is 30-50℃, the power is 150-250W, and the time is 30-60min; the centrifugation speed is 10000-14000g, and the time is 10-30min.

[0010] Preferably, in step S2, the ultrasonic extraction temperature is 35-40℃, the power is 200-220W, and the time is 40-50min.

[0011] Preferably, the Norway spruce leaf extract is prepared by water extraction, which can effectively extract the phenolic acid and polysaccharide components.

[0012] Secondly, the present invention provides an oil-controlling ingredient specifically formulated for men, characterized in that it is prepared by the preparation method of the oil-controlling ingredient specifically formulated for men in the first aspect.

[0013] Thirdly, the present invention provides the application of the oil-controlling ingredient specifically developed for men in the second aspect in the preparation of oil-controlling cosmetics.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: The oil-controlling ingredient of this invention, specifically formulated for men, is a fermentation product of *Pseudomonas aeruginosa* and *Picea spruce* leaves. Its mechanism of action for controlling oil production in men involves synergistic inhibition through a dual-pathway approach: Firstly, the fermentation product effectively inhibits the activity of type I 5α-reductase in the skin, blocking the conversion of testosterone to the more potent dihydrotestosterone (DHT), thus reducing the amplification of androgen signals at the source. Secondly, the product simultaneously downregulates the expression of androgen receptors (AR) or interferes with their binding to DHT and subsequent transcriptional activation, thereby weakening the sebaceous gland's response to androgens at the receptor level. Through this dual action of "inhibiting androgen activation" and "reducing receptor sensitivity," the oil-controlling ingredient of this invention achieves multi-level intervention on the androgen-sebaceous gland axis, fundamentally reducing excessive oil production caused by high androgen levels, rather than merely relying on surface adsorption or single enzyme inhibition. Furthermore, the *Pseudomonas aeruginosa* and *Picea spruce* leaf fermentation product is a naturally fermented source, making the ingredient mild and safe. This active ingredient is produced through a co-fermentation process using marine-derived *Pseudomonas alterniflora* and Norway spruce leaves. No chemically synthesized additives or irritating solvents are used throughout the process. The resulting product has been tested and is non-irritating to the skin, making it safe for people with sensitive skin to use. Attached Figure Description

[0015] Figure 1 This is a pseudo-color image of the distribution of oil on the forehead skin surface of volunteers in Example 1 and Comparative Example 3 before and after use in Test Example 3. Detailed Implementation

[0016] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0017] The raw materials used in the following examples and comparative examples are from the following sources: Pseudorabialis ATCC 19648: purchased from Beijing BioBio Biotechnology Co., Ltd. Pseudomonas alterniflora ATCC BAA-376: purchased from Beijing BioBio Biotechnology Co., Ltd. Norway spruce leaf extract: purchased from Shenzhen Diweijia Biotechnology Co., Ltd., product number DVJC056. Lactobacillus plantarum ATCC BAA-793: Purchased from Beijing BioBio Biotechnology Co., Ltd. Eucalyptus leaf extract: Manufacturer: Shaanxi Xinghe Zhiyan Biomedical Technology Co., Ltd., Product No.: XHZY20251016; Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0018] Example 1 A method for preparing an oil-controlling ingredient specifically formulated for men includes the following steps: S1. *Pseudomonas alterniflora* ATCC BAA-376 was inoculated into Marine Broth 2216 liquid medium (purchased from Thermo Fisher Scientific) at an inoculum of 3% (V / V), and fermented aerobicly at 24°C for 18 h until OD reached. 600 A value of 1.0 indicates the presence of *Pseudomonas alterniflora* bacterial suspension. S2. Sterilize the Norway spruce leaf extract by irradiation at a dose of 4 kGy to obtain sterile Norway spruce leaf extract. S3. Sterile Norway spruce leaf extract and Pseudomonas alterniflora bacterial culture were mixed at a mass ratio of 1:3 and fermented aerobicly at 24°C for 24 h, while maintaining the dissolved oxygen (DO) of the culture medium at 20% saturation to obtain the fermentation product. S4. The fermentation product was extracted using ultrasonic crushing at a temperature of 40°C and a power of 200W for 40 minutes. Then, it was centrifuged at 12000g for 20 minutes. The supernatant was collected and vacuum dried at 25°C to obtain the Pseudomonas alterniflora / Norwegian spruce leaf fermentation product, which is the oil-controlling ingredient specifically developed for men.

[0019] Example 2 A method for preparing an oil-controlling ingredient specifically formulated for men includes the following steps: S1. *Pseudomonas alterniflora* (ATCC 19648) was inoculated into Marine Broth 2216 liquid medium (purchased from Thermo Fisher Scientific) at an inoculum of 3% (V / V), and fermented aerobicly at 18°C ​​for 28 h until OD was reached. 600 A value of 1.2 indicates the presence of *Pseudomonas alterniflora* bacterial suspension. S2. Sterilize the Norway spruce leaf extract by irradiation at a dose of 4 kGy to obtain sterile Norway spruce leaf extract. S3. Sterile Norway spruce leaf extract and Pseudomonas alterniflora bacterial culture were mixed at a mass ratio of 1:2 and fermented aerobicly at 18°C ​​for 36 hours, while maintaining dissolved oxygen (DO) ≥ 20% saturation in the culture medium to obtain the fermentation product. S4. The fermentation product was extracted using ultrasonic crushing at a temperature of 30°C and a power of 220W for 50 minutes. Then, it was centrifuged at 12000g for 20 minutes. The supernatant was collected and vacuum dried at 25°C to obtain the Pseudomonas alterniflora / Norwegian spruce leaf fermentation product, which is the oil-controlling ingredient specially developed for men.

[0020] Example 3 The only difference between Example 3 and Example 1 is that in step S1, fermentation is carried out until the OD600 value is 0.6.

[0021] Example 4 The only difference between Example 4 and Example 1 is that in step S1, fermentation is carried out until the OD600 value is 1.5.

[0022] Example 5 The only difference between Example 5 and Example 1 is that in step S3, the aerobic fermentation time is 15 hours.

[0023] Example 6 The only difference between Example 6 and Example 1 is that in step S3, the aerobic fermentation time is 49 hours.

[0024] Example 7 The only difference between Example 7 and Example 1 is that in step S3, the dissolved oxygen (DO) value of aerobic fermentation is 14%.

[0025] Example 8 The only difference between Example 8 and Example 1 is that in step S3, the dissolved oxygen (DO) value of aerobic fermentation is 42%.

[0026] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that Lactobacillus plantarum ATCC BAA-793 was used instead of Pseudomonas alterniflora ATCC BAA-376.

[0027] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that Eucalyptus leaf extract was used instead of Norway spruce leaf extract.

[0028] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that the Norway spruce leaf extract and the Pseudomonas alterniflora fermentation product were directly mixed. The specific steps are as follows: S1. *Pseudomonas alterniflora* ATCC BAA-376 was inoculated into Marine Broth 2216 liquid medium (purchased from Thermo Fisher Scientific) at an inoculum of 3% (V / V), and fermented aerobicly at 24°C for 18 h until OD reached. 600 A value of 1.0 indicates the presence of *Pseudomonas alterniflora* bacterial suspension. S2. Continue aerobic fermentation of Pseudomonas alterniflora culture at 24°C for 24 h, keeping the dissolved oxygen (DO) of the culture medium at 20% saturation, to obtain the fermentation product of Pseudomonas alterniflora. S3. The fermentation product of Pseudomonas alterniflora was extracted by ultrasonic disruption at a temperature of 40°C and a power of 200W for 40 minutes. Then, it was centrifuged at 12000g for 20 minutes. The supernatant was collected and vacuum dried at 25°C to obtain the fermentation product extract of Pseudomonas alterniflora. S4. Sterilize the Norway spruce leaf extract by irradiation at a dose of 4 kGy to obtain sterile Norway spruce leaf extract. S5. Sterile Norway spruce leaf extract and Pseudomonas aeruginosa fermentation product extract were mixed at a mass ratio of 1:3 to obtain Comparative Example 3.

[0029] Test Example 1: In vitro experiments demonstrated that *Pseudomonas aeruginosa* / Norwegian spruce leaf fermentation products inhibit androgen-induced excessive sebum production in the skin. This study investigates the oil control effect of the samples prepared in Examples 1-8 and Comparative Examples 1-3: Experimental methods: The cell line used was human sebaceous gland cell line SZ95 (Shanghai Qingqi Biotechnology, BFN60807569), passaged 7 times, and cultured under the following conditions: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured according to groups, followed by testing, specifically the detection of neutral lipid content, 5α-reductase content, and androgen receptor content. The testing methods are as follows: (1) Seed the cell suspension into a 96-well cell culture plate at a density of 1.0 × 10⁶ cells per well. 4 Add 100 μL of DMEM medium (Gibco, C11965500BT) to each well and incubate for 24 h. (2) Discard the supernatant. Add 100 μL of DMEM medium containing 10 μM testosterone to the control group and add 100 μL of medium containing 10 μM testosterone and 0.1% w / v (g / mL) of the samples prepared in Examples 1-8 and Comparative Examples 1-3 to the sample groups respectively. (3) After incubating under the same conditions for another 48 h, collect the cells, discard the supernatant, and use the cells for subsequent detection; (4) Neutral lipid content detection: Nile red dye (Shanghai Yuanye Biotechnology, S02N11G129713) was added to the cells and incubated at 37℃ in the dark for 15 min. The cells were washed twice with PBS and the fluorescence OD value was detected using a multi-functional microplate reader. The excitation wavelength was 485 nm and the absorption wavelength was 565 nm. The relative content of neutral lipids in each group was calculated based on the fluorescence OD value. (5) Detection of 5α-reductase content: The 5α-reductase level of each group of cells was detected using the human 5α-reductase (5AR) ELISA kit (Shanghai Enzyme-Linked Biotechnology, ml064275). The specific operation was as follows: equal amounts of supernatant were added to the corresponding reaction wells of the kit. After incubation, washing, and color development, the absorbance OD value was measured at a specific wavelength using an ELISA reader. The 5α-reductase content of each group was calculated according to the standard curve. (6) Androgen receptor content detection: The androgen receptor level of each group of cells was detected using the human androgen receptor (AR) ELISA kit (Shanghai Enzyme-Linked Biotechnology, ml105494). The specific operation was as follows: equal amounts of supernatant were added to the corresponding reaction wells of the kit. After incubation, washing, and color development, the absorbance OD value was measured at a specific wavelength using an ELISA reader. The androgen receptor content of each group was calculated according to the standard curve.

[0030] The oil control effect of the samples is represented by the improvement of each value, and the calculation method is as follows: Sebum secretion improvement rate = (relative content of neutral lipids) 对照组 -Relative content of neutral lipids 样品组 ) / Relative content of neutral lipids 对照组 ×100%; 5α-Reductase Improvement Rate = (5α-Reductase Content) 对照组 -5α-reductase content 样品组 ) / 5α-reductase content 对照组 ×100%; androgen receptor improvement rate = (androgen receptor content) 对照组 -androgen receptor levels 样品组 ) / androgen receptor content 对照组 ×100%; the results are shown in Table 1. The higher the values ​​of the above three indicators, the more effectively the *Pseudomonas alterniflora* / Norwegian spruce leaf fermentation products can intervene in androgen-induced excessive sebum secretion through a multi-target synergistic mechanism (inhibition of 5α-reductase and downregulation of androgen receptors), and the better the oil control effect.

[0031] Table 1. Oil-controlling effect data of each oil-controlling ingredient Example 1 75.3 60.4 38.6 Example 2 74.0 59.7 38.0 Example 3 70.6 55.2 34.6 Example 4 56.7 45.0 28.7 Example 5 69.3 56.5 35.9 Example 6 58.5 47.3 29.6 Example 7 64.2 51.6 33.1 Example 8 61.4 49.2 31.8 Comparative Example 1 34.3 27.6 17.6 Comparative Example 2 41.0 32.9 20.1 Comparative Example 3 46.8 35.3 23.3 Based on the data from Examples 1 and 3-4 in Table 1, it can be seen that when OD600 ≤ 0.6 (insufficient bacterial count) or ≥ 1.5 (entering the stationary phase, intracellular enzymes release degradation active substances / pro-inflammatory), the oil control effect will be significantly reduced.

[0032] Based on the data from Examples 1 and 5-6 in Table 1, it can be seen that when the co-fermentation time is ≤16h, the substrate conversion is incomplete, and when it is ≥48h, the polyphenol oxidation polymerization will reduce the oil control activity.

[0033] Based on the data from Examples 1 and 7-8, it can be seen that co-fermentation with DO ≤ 15% causes local anaerobic conditions leading to a decrease in extracellular enzyme activity, while DO ≥ 40% leads to polyphenol oxidation and a weakened oil control effect (Examples 7 and 8).

[0034] Combining the data from Example 1 and Comparative Examples 1-3 in Table 1, it can be seen that in Comparative Example 1, replacing *Pseudomonas aeruginosa* with *Lactobacillus plantarum*, ordinary lactic acid bacteria do not possess the specific extracellular enzymes that can convert spruce polyphenols, and cannot generate dual-pathway oil-controlling active substances. The three indicators are significantly lower than in all examples, proving that *Pseudomonas aeruginosa* is the core functional strain for achieving oil-controlling effects. In Comparative Example 2, replacing Norway spruce leaf extract with eucalyptus leaf extract, it lacks a characteristic polyphenol substrate that can be converted by the strain and target the androgen-sebaceous gland axis. The fermentation product has no obvious oil-controlling activity, proving that Norway spruce leaf extract is the complementary functional substrate. In Comparative Example 3, only physical mixing without co-fermentation, simple mixing cannot rely on the enzymatic hydrolysis of plant raw materials by the strain, and cannot simultaneously achieve the synergistic effect of inhibiting 5α-reductase and downregulating androgen receptors. Only weak basic activity of a single raw material exists, and the overall oil-controlling effect is the worst in the entire group, proving that co-fermentation of the strain and plant extract is a necessary process to produce strong oil-controlling activity, and simple compounding cannot achieve the same effect.

[0035] In summary, the optimal oil-control effect occurred when *Pseudomonas alterniflora* was inoculated into Marine Broth 2216 and fermented aerobically to an OD600 of 1.0–1.2 (logarithmic growth phase), with Norway spruce leaf extract and bacterial broth co-fermented at 18–26°C for 16–48 hours and dissolved oxygen (DO) of 15–40%. Under these conditions, the *Pseudomonas alterniflora* / Norwegian spruce leaf fermentation product exhibited the strongest inhibition rate against type I 5α-reductase and the greatest ability to downregulate / interfere with androgen receptor (AR) expression, resulting in the highest improvement rate in sebum secretion. This indicates that the *Pseudomonas alterniflora* / Norwegian spruce leaf fermentation product of this invention can simultaneously achieve two oil-control pathways: inhibiting the conversion of testosterone to DHT at the source and reducing the sebaceous gland response to androgens at the receptor level, unlike ordinary adsorption-type oil-control ingredients; moreover, it contains no chemically irritating additives throughout the process, and the fermentation product is mild and non-irritating, suitable for men with oily and sensitive skin.

[0036] Test Example 2: Human Skin Patch Test.

[0037] Sixty volunteers were recruited, 30 men and 30 women, aged 20-50 years, and randomly divided into two groups of 15 men and 15 women each. A closed patch test method was used. Equal volumes (0.020 mL-0.025 mL) of the test sample (containing 1% w / v aqueous solutions of the samples prepared in Examples 1-8 and Comparative Examples 1-3) were placed in a specific patch applicator. The patch was then applied to the volunteer's arm with hypoallergenic adhesive tape, gently pressed to ensure even application, and left for 24 hours. The blank control group used distilled water, and the blank application examples used a serum without the whitening composition. One patch applicator was applied to each arm of each volunteer. Each patch applicator had 10 wells, allowing for the simultaneous testing of 20 samples per volunteer (both arms). The test samples for the first group were the sample solutions prepared in Examples 1-8, and the test samples for the second group were the sample solutions prepared in Comparative Examples 1-3. A blank control was included in each group. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 2 below.

[0038] Table 2 Adverse Reaction Grades After testing, the samples provided in Examples 1-8 and Comparative Examples 1-3 of this invention all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0039] Test Example 3: Human efficacy test of each composition for oil control Experimental Methods: Sixty-six participants aged 18-40 with oily, sensitive skin (who reported feeling sensitive and had an oil content on their forehead >120 μg / cm²) were selected. 2 Asian adult male participants were randomly divided into 11 groups of 6. Each group of volunteers was randomly assigned to use a test sample (containing 1% w / v aqueous solution of the samples prepared in Examples 1-8 and Comparative Examples 1-3). Volunteers applied equal amounts of the sample to their entire face twice daily, morning and evening. Data were collected before use (D0), after 7 days of use (D7), after 14 days of use (D28), and after 7 days of discontinuation (DT7). After arriving at the trial site, volunteers washed their faces with facial cleanser and then sat quietly for 2 hours in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%. Researchers then used a skin oil testing probe (Sebumeter SM 815) to collect data on the oil content of the forehead.

[0040] Skin's oil control ability is represented by the improvement in forehead oil content, as shown in the following formula: Oil content improvement rate = (oil content D0 - oil content Dx) / oil content D0 × 100%; data are shown in Table 3.

[0041] Table 3. Test data on the human oil-controlling efficacy of each composition.

[0042] As shown in Tables 2-3, the oil improvement rates of the compositions in Examples 1 and 2 reached 37.9% and 37.4% respectively at D14, significantly better than other groups. This indicates that when *Pseudomonas aeruginosa* was fermented to the logarithmic growth phase (OD600 = 1.0-1.2) to obtain *Pseudomonas aeruginosa* broth, and co-fermented with Norway spruce leaf extract under conditions of 18-26℃, DO 15-40%, and fermentation for 16-48 h, the product had the highest content of active molecules, which could efficiently regulate sebaceous gland secretion through a dual pathway of "inhibition of 5α-reductase + downregulation of androgen receptors". Seven days after discontinuation, the improvement rates of both groups remained above 36%, proving that the component has a sustained regulatory effect on the androgen-sebaceous gland axis, rather than a transient physical adsorption.

[0043] Depend on Figure 1 As can be seen, the image was acquired using the skin surface texture analysis system (Visioscan@VC 20plus) in conjunction with the Sebufix skin oil testing membrane. The black, yellow-green, red, and blue heterogeneous patches in the image represent sebum particles of different sizes adsorbed by the Sebufix testing membrane. After 7 and 14 days of use in volunteers of Example 1, the number of sebum particles was significantly lower than in Comparative Example 3. Furthermore, after 7 days of discontinuation, there was no significant change in the number of sebum particles in volunteers using Comparative Example 3. However, after 7 days of discontinuation, the number of sebum particles in volunteers using Comparative Example 3 showed a significant upward trend compared to 14 days of use, indicating that the oil-controlling ingredient in Example 1 has a long-lasting oil-controlling effect.

[0044] In summary, the aqueous solution of the co-fermentation product of *Pseudomonas alterniflora* and *Picea spruce* leaves of this invention showed good oil-controlling effects in male subjects with oily and sensitive skin, with no reports of irritation. It is suitable for daily care of men with oily, especially sensitive, skin.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing an oil-controlling ingredient specifically formulated for men, characterized in that, Includes the following steps: S1. The *Pseudomonas aeruginosa* bacterial culture and Norway spruce leaf extract were mixed and subjected to aerobic fermentation to obtain the fermentation product; wherein the inoculum amount of *Pseudomonas aeruginosa* was 1-5% of the mass of the Norway spruce leaf extract. S2. The fermentation product obtained in step S1 is extracted by ultrasonication, centrifuged, the supernatant is collected and dried to obtain the Pseudomonas alterniflora / Norwegian spruce leaf fermentation product, which is the oil-controlling ingredient specially developed for men.

2. The preparation method of the oil-controlling ingredient specifically formulated for men as described in claim 1, characterized in that, In step S1, the temperature for aerobic fermentation is 18-26℃, the dissolved oxygen content is 15-40%, and the time is 16-48h.

3. The preparation method of the oil-controlling ingredient specifically developed for men as described in claim 1, characterized in that, The method for preparing the *Pseudomonas alterniflora* bacterial culture includes the following steps: inoculating *Pseudomonas alterniflora* into a marine culture medium and performing aerobic fermentation until the OD600 value is 0.6-1.5 to obtain the *Pseudomonas alterniflora* bacterial culture; wherein, the temperature of aerobic fermentation is 18-26℃ and the time is 18-36h.

4. The preparation method of the oil-controlling ingredient specifically developed for men as described in claim 1, characterized in that, The pseudoalternating monoclonal bacteria are pseudoalternating monoclonal bacteria ATCC 19648 and / or pseudoalternating monoclonal bacteria ATCC BAA-376.

5. The preparation method of the oil-controlling ingredient specifically developed for men as described in claim 1, characterized in that, In step S2, the ultrasonic extraction temperature is 30-50℃, the power is 150-250W, and the time is 30-60min; the centrifugation speed is 10000-14000g, and the time is 10-30min.

6. The preparation method of the oil-controlling ingredient specifically developed for men as described in claim 1, characterized in that, In step S2, the ultrasonic extraction temperature is 35-40℃, the power is 200-220W, and the time is 40-50min.

7. An oil-controlling ingredient specifically developed for men, characterized in that, It is prepared by the method for preparing the oil-controlling ingredient specifically for men according to any one of claims 1-6.

8. The application of the oil-controlling ingredient specifically developed for men as described in claim 7 in the preparation of oil-controlling cosmetics.

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