A cell separation and extraction device

Abstract

This invention relates to the field of leukemia blood cell separation technology, and more particularly to a cell separation and extraction device. The invention includes: a machine base, with a housing fixed to the top of the machine base, and a separation chamber mounted on the top of the housing; a separation mechanism disposed between the separation chamber and the housing; and a sampling mechanism disposed on the separation chamber. Through the structural design of the separation and oscillation mechanisms, this invention superimposes axial high-frequency micro-amplitude vibration on top of traditional centrifugal force, solving the problem of abnormal white blood cell aggregation in leukemia patient blood samples. This allows cells of similar density to more accurately form independent stratifications along the medium, making it particularly suitable for the fine separation of leukemia cell subpopulations. Furthermore, the target cells are extracted through the sampling mechanism. The entire extraction process is completed within the sealed separation chamber, avoiding contact between the sample and the external environment, effectively preventing microbial contamination, and offering high tolerance and separation purity. It is suitable for cytological testing and subsequent treatment of leukemia patients.

Description

Technical Field

[0001] This invention relates to the field of leukemia blood cell separation technology, and more particularly to a cell separation and extraction device. Background Technology

[0002] In common hematology settings, leukemia is a malignant tumor originating from the hematopoietic system, primarily characterized by the proliferation of abnormal white blood cells, affecting the production of normal blood cells. In the clinical diagnosis, subtyping, and subsequent treatment research of leukemia, isolating and extracting high-purity target cells (such as leukemia cells, mononuclear cells, etc.) from patient blood samples is a fundamental and crucial procedure. Currently, commonly used cell isolation methods in clinical practice mainly include density gradient centrifugation and immunomagnetic bead sorting. Among these, density gradient centrifugation is widely used due to its relatively simple operation and low cost.

[0003] For example, a cell separation and extraction device for blood disease testing, with publication (announcement) number CN113174316A, includes test tubes and a motor. The bottom surface of the test tubes is arranged in a parallel shape and has an insertion port. The test tubes are fitted with one or more sealing rings. The outer wall surface of the test tubes has one or more liquid outlet holes. Liquid outlet tubes are installed on the outer ring surface of the sealing tubes. Each liquid outlet tube is fitted with a cover. A connecting sleeve is installed on the end face of each cover. A panel is installed on the rotating shaft of the motor. An insertion rod is installed on the panel and inserted into the insertion port. A vertical plate is provided on the left side of the test tube. The upper end of the vertical plate is bent to form a positioning part, which abuts against the top surface of the cover on the test tube.

[0004] In summary, the existing technology has the following technical problems: During use, the blood samples from leukemia patients show an abnormally high number of white blood cells, which often aggregate. During conventional centrifugation, the aggregated cell clumps settle unevenly, easily leading to unclear stratification interfaces and affecting separation purity. Furthermore, the existing technology uses a structure with perforations on the outer wall of the test tube and a dynamically sealed rubber ring. During centrifugation, there is high-speed relative sliding between the rubber sealing ring and the perforated test tube wall, making the rubber susceptible to shear damage from the edges of the perforations, resulting in particulate contamination. Moreover, the sealing reliability is poor over long-term use. Therefore, we propose a cell separation and extraction device. Summary of the Invention

[0005] The purpose of this invention is to provide a cell separation and extraction device to solve the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: A cell separation and extraction device, comprising: A machine base, the top of which is fixed with a housing, and the top of the housing is equipped with a separation chamber; A separation mechanism, located between the separation chamber and the housing, is used to centrifuge and separate blood samples within the separation chamber. A sampling mechanism, located on the separation chamber, is used to extract target cells from the stratified blood sample.

[0007] Preferably, the separation mechanism includes a drive assembly and a mating assembly. A base is fixed to the bottom of the inner side of the housing, and support arms are fixed to both ends of the top of the base. A drive assembly is assembled between the two support arms, and a mating assembly is assembled between the drive assembly and the support arms.

[0008] Preferably, the drive assembly includes a first motor, a first pulley, a transmission belt, a second pulley, a positioning frame, a positioning cylinder, a telescopic rod, and a top plate. One side of one of the support arms is fixed to the first motor, the output end of the first motor is fixed to the first pulley, the outer side of the first pulley is connected to the second pulley via the transmission belt, the inner side of the second pulley is rotatably connected to the positioning frame, both ends of the bottom of the positioning frame are fixed to the support arm, both ends of the top of the second pulley are fixed to the positioning cylinder, the inner side of each positioning cylinder is slidably connected to the telescopic rod, and the top of the two telescopic rods is fixed to a top plate, which is slidably connected to the top of the housing.

[0009] Preferably, the mating assembly includes a clamp, an inner cavity, and a square rod. The clamp is fixed to the top of the top plate, the separation cavity is clamped and fixed inside the clamp, the positioning frame has an inner cavity on its inner side, and a square rod is slidably connected to the inner side of the inner cavity. The top of the square rod is assembled and connected to the top plate.

[0010] Preferably, a partition plate is fixed to the top of the square rod, the bottom of the partition plate is in close contact with the top of the positioning frame, a top column is fixed to the top of the partition plate, and a sleeve is rotatably connected to the top of the top column through a bearing, and the top of the sleeve is fixed to the top plate.

[0011] Preferably, the sampling mechanism includes a sealing cap, a sampling tube, a drainage head, and a valve. The top of the separation chamber is provided with a sealing cap, and the top of the sealing cap is provided with a vent valve. Multiple sampling tubes are evenly distributed and fixed on one side of the separation chamber. One end of each sampling tube is threadedly connected to a drainage head, and the top of each sampling tube is provided with a valve.

[0012] Preferably, the sampling mechanism further includes an electric push rod, a protective plate, a limiting through hole, and a controller. Both ends of the top of the machine are fixed with electric push rods, and the output ends of the two electric push rods are fixed with protective plates. The inner side of the protective plate is provided with a limiting through hole corresponding to the position of the sealing cap. The limiting through hole slides in contact with the sealing cap. A controller is fixed on one side of the machine housing. The controller is electrically connected to the electric push rod and the first motor through a wire.

[0013] Preferably, an oscillation mechanism is assembled between the base and the square rod, the oscillation mechanism being used to perform slight vertical oscillation on the separation cavity.

[0014] Preferably, the oscillation mechanism includes a mounting plate, a second motor, a small cam, a swing arm, a force-guiding roller, a U-shaped plate, a force-applying rod, and a connecting ball. The mounting plate is fixed to the top of the base, and the second motor is fixed to one side of the mounting plate. The output end of the second motor passes through the mounting plate and is fixed to the small cam. The swing arm is rotatably connected to one side of the mounting plate and offset from the position of the second motor. The force-guiding roller is rotatably connected to the inner side of the swing arm. The bottom of the force-guiding roller is in contact with the small cam. One end of the swing arm is rotatably connected to the U-shaped plate. The force-applying rod is fixed to the top of the U-shaped plate. The connecting ball is rotatably connected to the top of the force-applying rod and is in ball joint with the square rod.

[0015] It is clear without a doubt that the technical solution described above in this application can solve the technical problem that this application aims to address.

[0016] Meanwhile, through the above technical solutions, the present invention has at least the following beneficial effects: 1. This invention, through the structural design of the separation mechanism and the oscillation mechanism, superimposes axial high-frequency micro-amplitude vibration on the basis of traditional centrifugal force, which solves the problem of abnormal white blood cells easily agglomerating in blood samples of leukemia patients, and enables cells with similar densities to form independent layers more accurately along the medium, which is particularly suitable for the fine separation of leukemia cell subpopulations.

[0017] 2. Through the structural design of the sampling mechanism, this invention enables the device to extract target cells. The entire extraction process is completed within a sealed separation chamber, avoiding contact between the sample and the external environment, effectively preventing microbial contamination, and providing high tolerance and separation purity. It is suitable for cytological testing and subsequent treatment of leukemia patients. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a cross-sectional structural diagram of the housing of the present invention; Figure 3 This is a schematic diagram of the connection structure between the base and the support arm of the present invention; Figure 4 This is a cross-sectional structural diagram of the support arm and positioning frame of the present invention; Figure 5 This is a schematic diagram of the connection structure between the mounting plate and the second motor of the present invention; Figure 6 This is a schematic diagram of the connection structure between the separation chamber and the sampling tube of the present invention.

[0020] The attached diagram lists the components represented by each number as follows: In the diagram: 1. Machine base; 2. Machine housing; 3. Separation chamber; 4. Base; 5. Support arm; 6. First motor; 7. First pulley; 8. Transmission belt; 9. Second pulley; 10. Positioning frame; 11. Positioning cylinder; 12. Telescopic rod; 13. Top plate; 14. Clamp; 15. Inner cavity; 16. Square rod; 17. Partition plate; 18. Top column; 19. Sleeve; 20. Mounting plate; 21. Second motor; 22. Small cam; 23. Swing arm; 24. Guide roller; 25. U-shaped plate; 26. Force rod; 27. Connecting ball; 28. Sealing cap; 29. ​​Sampling tube; 30. Drainage head; 31. Valve; 32. Electric push rod; 33. Protective plate; 34. Limiting through hole; 35. Controller. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. Example 1

[0022] Reference Figure 1-6 A cell separation and extraction device, comprising: Machine base 1, with a housing 2 fixed on the top of the machine base 1, and a separation chamber 3 assembled on the top of the housing 2; The separation mechanism is located between the separation chamber 3 and the housing 2, and is used to centrifuge and separate blood samples in the separation chamber 3. The sampling mechanism, located on the separation chamber 3, is used to extract target cells from the stratified blood sample, providing an integrated device that combines centrifugation stratification and sealed sampling. The entire extraction process is completed within the sealed separation chamber 3, effectively preventing the sample from contacting the external environment, reducing the risk of microbial contamination, and offering convenient and efficient operation.

[0023] The separation mechanism includes a drive assembly and a mating assembly. A base 4 is fixed to the bottom inside the housing 2. Support arms 5 are fixed to both ends of the top of the base 4. A drive assembly is assembled between the two support arms 5. A mating assembly is assembled between the drive assembly and the support arms 5. This layout makes the transmission path of rotational power short and the rigidity high, and the centrifugal operation smooth, effectively improving the stability of equipment operation.

[0024] The drive assembly includes a first motor 6, a first pulley 7, a transmission belt 8, a second pulley 9, a positioning frame 10, a positioning cylinder 11, a telescopic rod 12, and a top plate 13. The first motor 6 is fixed to one side of one of the support arms 5. The first pulley 7 is fixed to the output end of the first motor 6. The second pulley 9 is connected to the outer side of the first pulley 7 via the transmission belt 8. The positioning frame 10 is rotatably connected to the inner side of the second pulley 9. Both ends of the bottom of the positioning frame 10 are fixed to the support arm 5. The positioning cylinder 11 is fixed to both ends of the top of the second pulley 9. The telescopic rod 12 is slidably connected to the inner side of the positioning cylinder 11. A top plate 13 is fixed to the top of the two telescopic rods 12. The top plate 13 is slidably connected to the top of the housing 2. The sliding fit between the positioning cylinder 11 and the telescopic rod 12, and the sliding contact between the top plate 13 and the top of the housing 2, ensure that the separation chamber 3 maintains good alignment during high-speed rotation and can adapt to separation chambers 3 of different heights, making it highly versatile.

[0025] The mating components include a clamp 14, an inner cavity 15, and a square rod 16. The clamp 14 is fixed to the top of the top plate 13, and the separation cavity 3 is clamped and fixed inside the clamp 14. The inner cavity 15 is opened on the inner side of the positioning frame 10, and the square rod 16 is slidably connected to the inner side of the inner cavity 15. The top of the square rod 16 is assembled and connected to the top plate 13. The inner cavity 15 opened on the inner side of the positioning frame 10 is slidably engaged with the square rod 16. This structure can effectively transmit rotational torque to drive the top plate 13 and the separation cavity 3 to rotate centrifugally, and also allows the square rod 16 to slide freely along the axial direction in the inner cavity 15, providing a reliable motion coupling basis for subsequent superposition of axial vibration. The structure is simple and efficient.

[0026] A partition plate 17 is fixed to the top of the square rod 16. The bottom of the partition plate 17 is in close contact with the top of the positioning frame 10. A top column 18 is fixed to the top of the partition plate 17. A sleeve 19 is rotatably connected to the top of the top column 18 via a bearing. The top of the sleeve 19 is fixed to the top plate 13. By fixing the partition plate 17 to the top of the square rod 16 and making the bottom of the partition plate 17 in close contact with the top of the positioning frame 10, axial limiting is achieved to prevent the square rod 16 from moving during rotation. The top column 18 is rotatably connected to the sleeve 19 via a bearing. The top of the sleeve 19 is fixed to the top plate 13. This bearing connection method effectively reduces rotational friction loss and ensures the concentricity between the top plate 13 and the square rod 16, making the centrifugal rotation smoother and more stable.

[0027] The sampling mechanism includes a sealing cap 28, sampling tubes 29, a drainage head 30, and a valve 31. A sealing cap 28 is installed at the top of the separation chamber 3, and a vent valve is installed at the top of the sealing cap 28. Multiple sampling tubes 29 are evenly distributed and fixed on one side of the separation chamber 3. One end of each sampling tube 29 is threadedly connected to a drainage head 30, and a valve 31 is installed at the top of each sampling tube 29. The sealing cap 28 with a vent valve at the top of the separation chamber 3 maintains the pressure balance inside and outside the chamber. Multiple sampling tubes 29 are evenly distributed on one side of the separation chamber 3, and each sampling tube 29 is threadedly connected to a drainage head 30 and equipped with a valve 31. The operator can select the sampling tube 29 of the corresponding height for sampling based on the clear layered interface after centrifugation. The drainage head 30 is replaceable to adapt to different collection containers. The sampling process is sealed and flexible, making it particularly suitable for the precise extraction of different cell subpopulations from precious blood samples from leukemia patients.

[0028] The sampling mechanism also includes an electric push rod 32, a protective plate 33, a limiting through hole 34, and a controller 35. Electric push rods 32 are fixed at both ends of the top of the machine base 1. Protective plates 33 are fixed to the output ends of both electric push rods 32. A limiting through hole 34 corresponding to the position of the sealing cap 28 is opened on the inner side of the protective plate 33. The limiting through hole 34 slides in contact with the sealing cap 28. A controller 35 is fixed to one side of the machine housing 2. The controller 35 is electrically connected to the electric push rods 32 and the first motor 6 via wires. Before separation, the controller 35 controls the output ends of the two electric push rods 32 to retract, causing the protective plate 33 to move. This allows the limiting through hole 34 on the protective plate 33 to slide in contact with the sealing cap 28, ensuring the stability of the separation chamber 3. This facilitates circumferential limiting of the sealing cap 28 during separation, making the separation chamber 3 more stable during rotation. Example 2

[0029] Further optimizations to Example 1, specifically, such as... Figure 5 As shown, an oscillation mechanism is assembled between the base 4 and the square rod 16. The oscillation mechanism is used to perform slight vertical oscillation on the separation chamber 3.

[0030] The oscillation mechanism includes a mounting plate 20, a second motor 21, a small cam 22, a swing arm 23, a guide roller 24, a U-shaped plate 25, a force-applying rod 26, and a connecting ball 27. The mounting plate 20 is fixed to the top of the base 4. The second motor 21 is fixed to one side of the mounting plate 20. The output end of the second motor 21 passes through the mounting plate 20 and is fixed to the small cam 22. The swing arm 23 is rotatably connected to one side of the mounting plate 20, offset from the position of the second motor 21. The guide roller 24 is rotatably connected to the inner side of the swing arm 23. The bottom of the guide roller 24 is in contact with the small cam 22. One end of the swing arm 23 is rotatably connected to a U-shaped plate 25. A force-applying rod 26 is fixed to the top of the U-shaped plate 25, and a connecting ball 27 is rotatably connected to the top of the force-applying rod 26. The connecting ball 27 is ball-jointed with a square rod 16. A second motor 21 is fixed through a mounting plate 20. The second motor 21 drives a small cam 22 to rotate. The small cam 22 periodically pushes the guide roller 24, causing the swing arm 23 to swing back and forth around the rotation point. The swing is then converted into linear reciprocating motion through the U-shaped plate 25 and the force-applying rod 26, and transmitted to the separation chamber 3 through the ball-joint connection between the connecting ball 27 and the square rod 16. This oscillation mechanism has a compact structure, efficiently converting rotational motion into high-frequency micro-amplitude axial vibration. The amplitude is adjustable and the impact is small, which will not damage cell activity. At the same time, the ball-joint method allows for a certain degree of installation eccentricity, reducing the requirements for processing and assembly precision, resulting in high reliability and suitability for long-term use in medical testing scenarios.

[0031] In summary: This invention addresses the following technical problem: In existing technologies, due to the abnormally high number of white blood cells in blood samples from leukemia patients, and the cells often exhibiting aggregated states, the uneven settling speed of these aggregated cell clumps during conventional centrifugation easily leads to unclear stratification interfaces, affecting separation purity. Furthermore, existing technologies employ a structure with perforations in the outer wall of the test tube and a dynamically sealed rubber ring. During centrifugation, high-speed relative sliding occurs between the rubber sealing ring and the perforated test tube wall, making the rubber susceptible to shear damage from the edges of the perforations, resulting in particulate contamination. Moreover, the sealing reliability is poor over long-term use. The invention employs the technical solutions described in the above embodiments. The implementation process of the above technical solutions is as follows: Sample loading and installation: The blood sample to be processed, pre-added with density gradient separation solution, is loaded into the separation chamber 3, and the sealing cap 28 is tightened. The vent valve at the top of the sealing cap 28 maintains internal air pressure balance. Then, the separation chamber 3 is placed into the clamp 14 and fixed by the clamp 14, so that the separation chamber 3 and the top plate 13 are integrated.

[0032] Centrifugal stratification and oscillatory deagglomeration: The first motor 6 is started by the controller 35. The first motor 6 drives the first pulley 7, which in turn drives the second pulley 9 to rotate via the transmission belt 8. The second pulley 9 drives the telescopic rod 12 to rotate via the positioning cylinder 11, which in turn causes the top plate 13 to rotate synchronously. The top plate 13 and the separation chamber 3 fixed on it rotate at high speed, generating centrifugal force, causing the blood sample to stratify according to density.

[0033] Simultaneously or intermittently, the second motor 21 is activated, driving the small cam 22 to rotate. The small cam 22 periodically pushes the guide roller 24, causing the swing arm 23 to swing back and forth. Through the U-shaped plate 25, the force rod 26, and the connecting ball 27, the swing is converted into vertical high-frequency micro-amplitude vibration of the square rod 16. This vibration, superimposed on the centrifugal motion, effectively breaks up abnormal white blood cell clusters, making the interfaces of each cell layer clear. After centrifugation and oscillation, the first motor 6 and the second motor 21 are turned off.

[0034] Layered sampling: Based on the layer of the target cells, such as the mononuclear cell layer or the leukemia cell layer, select the sampling tube 29 at the corresponding height. Open the valve 31 at the top of the sampling tube 29, connect the drainage head 30 to an external syringe or negative pressure device, and slowly draw fluid from the target cell layer. Since the sampling tubes 29 are evenly distributed along the side wall of the separation chamber 3, and the drainage heads 30 can be threaded and replaced, precise sampling at different layers can be achieved.

[0035] Collection and cleaning: After sampling, close valve 31, remove drainage head 30, loosen clamp 14 and remove separation chamber 3, and clean and disinfect the device for future use.

[0036] With the above-mentioned settings, this application will certainly solve the above-mentioned technical problems, and at the same time achieve the following technical effects: 1. This invention, through the structural design of the separation mechanism and the oscillation mechanism, superimposes axial high-frequency micro-amplitude vibration on the basis of traditional centrifugal force, which solves the problem of abnormal white blood cells easily agglomerating in blood samples of leukemia patients, and enables cells with similar densities to form independent layers more accurately along the medium, which is particularly suitable for the fine separation of leukemia cell subpopulations.

[0037] 2. Through the structural design of the sampling mechanism, this invention enables the device to extract target cells. The entire extraction process is completed within the sealed separation chamber 3, avoiding contact between the sample and the external environment, effectively preventing microbial contamination, and providing high fault tolerance and high separation purity. It is suitable for cytological testing and subsequent treatment of leukemia patients.

[0038] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0039] Obviously, the embodiments described above are merely some embodiments of the present invention, not all embodiments. The accompanying drawings show preferred embodiments of the present invention, but do not limit the patent scope of the present invention. The present invention can be implemented in many different forms; rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing specific embodiments, or make equivalent substitutions for some of the technical features. Any equivalent structures made using the content of this specification and drawings, directly or indirectly applied to other related technical fields, are similarly within the patent protection scope of this invention.

Claims

1. A cell separation and extraction device, characterized in that, include: A machine base (1) is fixed to the top of the machine base (1), and a separation chamber (3) is assembled on the top of the machine housing (2). A separation mechanism is provided between the separation chamber (3) and the housing (2) for centrifuging and stratifying the blood sample in the separation chamber (3); A sampling mechanism is provided on the separation chamber (3) for extracting target cells from the stratified blood sample.

2. The cell separation and extraction device according to claim 1, characterized in that, The separation mechanism includes a drive assembly and a mating assembly. A base (4) is fixed to the bottom of the inner side of the housing (2). Support arms (5) are fixed to both ends of the top of the base (4). A drive assembly is assembled between the two support arms (5). A mating assembly is assembled between the drive assembly and the support arms (5).

3. The cell separation and extraction device according to claim 2, characterized in that, The drive assembly includes a first motor (6), a first pulley (7), a transmission belt (8), a second pulley (9), a positioning frame (10), a positioning cylinder (11), a telescopic rod (12), and a top plate (13). The first motor (6) is fixed to one side of one of the support arms (5). The first pulley (7) is fixed to the output end of the first motor (6). The second pulley (9) is connected to the outer side of the first pulley (7) via the transmission belt (8). The positioning frame (10) is rotatably connected to the inner side of the second pulley (9). The two ends of the bottom of the positioning frame (10) are fixed to the support arm (5). The two ends of the top of the second pulley (9) are fixed to the positioning cylinder (11). The telescopic rod (12) is slidably connected to the inner side of the positioning cylinder (11). A top plate (13) is fixed to the top of the two telescopic rods (12). The top plate (13) is slidably connected to the top of the housing (2).

4. The cell separation and extraction device according to claim 3, characterized in that, The mating assembly includes a clamp (14), an inner cavity (15), and a square rod (16). The clamp (14) is fixed to the top of the top plate (13). The separation cavity (3) is clamped and fixed inside the clamp (14). The inner cavity (15) is opened on the inner side of the positioning frame (10). The square rod (16) is slidably connected to the inner side of the inner cavity (15). The top of the square rod (16) is assembled and connected to the top plate (13).

5. The cell separation and extraction device according to claim 4, characterized in that, The top of the square rod (16) is fixed with a partition plate (17), the bottom of the partition plate (17) is in contact with the top of the positioning frame (10), the top of the partition plate (17) is fixed with a top column (18), the top of the top column (18) is rotatably connected to a sleeve (19) through a bearing, and the top of the sleeve (19) is fixed to the top plate (13).

6. The cell separation and extraction device according to claim 3, characterized in that, The sampling mechanism includes a sealing cap (28), a sampling tube (29), a drainage head (30), and a valve (31). The top of the separation chamber (3) is provided with a sealing cap (28), and the top of the sealing cap (28) is provided with a vent valve. Multiple sampling tubes (29) are evenly distributed and fixed on one side of the separation chamber (3). One end of each sampling tube (29) is threadedly connected to a drainage head (30), and the top of each sampling tube (29) is provided with a valve (31).

7. The cell separation and extraction apparatus according to claim 6, characterized in that, The sampling mechanism also includes an electric push rod (32), a protective plate (33), a limiting through hole (34), and a controller (35). Both ends of the top of the machine base (1) are fixed with electric push rods (32), and the output ends of the two electric push rods (32) are fixed with protective plates (33). The inner side of the protective plate (33) is provided with a limiting through hole (34) corresponding to the position of the sealing cap (28). The limiting through hole (34) slides in contact with the sealing cap (28). A controller (35) is fixed on one side of the machine housing (2). The controller (35) is electrically connected to the electric push rod (32) and the first motor (6) through a wire.

8. The cell separation and extraction device according to claim 4, characterized in that, An oscillation mechanism is assembled between the base (4) and the square rod (16), which is used to perform slight vertical oscillation on the separation cavity (3).

9. The cell separation and extraction device according to claim 8, characterized in that, The oscillation mechanism includes a mounting plate (20), a second motor (21), a small cam (22), a swing arm (23), a guide roller (24), a U-shaped plate (25), a force-applying rod (26), and a connecting ball (27). The mounting plate (20) is fixed to the top of the base (4). The second motor (21) is fixed to one side of the mounting plate (20). The output end of the second motor (21) passes through the mounting plate (20) and is fixed to the small cam (22). The oscillation mechanism is located on one side of the mounting plate (20) and is offset from the first motor (23). The position of the two motors (21) is rotatably connected to a swing arm (23). The inner side of the swing arm (23) is rotatably connected to a guide roller (24). The bottom of the guide roller (24) is in contact with the small cam (22). One end of the swing arm (23) is rotatably connected to a U-shaped plate (25). The top of the U-shaped plate (25) is fixed with a force rod (26). The top of the force rod (26) is rotatably connected to a connecting ball (27). The connecting ball (27) is ball-jointed with the square rod (16).

Images