Microbial inoculant automatic propagation device

Abstract

This invention relates to the field of microbial cultivation technology, specifically an automated microbial agent propagation device, including a fermenter. A support component is fixedly installed inside the fermenter, and a stirring mechanism is connected to the support component. The stirring mechanism is used to stir and dissolve oxygen in the bacterial solution within the fermenter. This invention uses a rotating shaft to drive several sets of zipper-type paddles to rotate at low speed, causing the paddles to stir the bacterial solution. Simultaneously, the paddles push the bacterial solution upwards and downwards, while the aeration block discharges micro-oxygen bubbles to the back surface of the paddles. Then, a figure-eight plate gathers the bacterial solution to the back surface of the paddles, thereby forcing the bacterial solution and micro-oxygen bubbles to mix and dissolve. While maintaining low-speed operation, efficient gas-liquid mixing and oxygen dissolution are achieved, meeting the high oxygen uptake requirements of aerobic probiotics while avoiding damage to the bacteria due to high shear stress.

Description

Technical Field

[0001] This invention relates to the field of microbial cultivation technology, specifically to an automated microbial agent propagation device. Background Technology

[0002] Probiotics are a class of live microorganisms that are beneficial to the health of the host. They are widely used in agricultural planting, animal husbandry, aquaculture, food fermentation and environmental remediation. Probiotics represented by Bacillus subtilis have become the core strains in probiotic preparations due to their excellent characteristics such as spore production, storage resistance, secretion of various enzymes and antibacterial substances.

[0003] These probiotics are strict aerobic bacteria, requiring a continuous and efficient oxygen supply to meet their high metabolic activity during liquid propagation, especially during the logarithmic growth phase, where the peak oxygen uptake rate can reach 30-50 mmol O2 / (L·h). Meanwhile, rod-shaped probiotics, represented by Bacillus subtilis, have a cell length of approximately 2-5 μm and a diameter of approximately 0.8-1.2 μm. Their long axis is more susceptible to damage from flow field shear stress, which in turn reduces the viability and spore formation rate.

[0004] Existing propagation devices mainly break up large bubbles and increase gas-liquid contact by rotating the agitator. However, to obtain a sufficient dissolved oxygen rate, it is usually necessary to increase the agitation speed to 800-1200 rpm, at which point the shear rate generated at the blade edge can reach 200-500 s. -1 The concentration of bacteria far exceeds the safety threshold of Bacillus subtilis, resulting in damage to a large number of bacteria. If the stirring speed is reduced to protect the bacteria (e.g., below 100 rpm), the bubble residence time is short, the gas-liquid mass transfer coefficient decreases, and the dissolved oxygen concentration is insufficient to maintain the normal growth requirements of the bacteria. This ultimately results in a longer propagation cycle, lower live bacteria yield, and large batch-to-batch differences.

[0005] Therefore, there is an urgent need for an automated propagation device that can maintain low-speed operation, effectively reduce shear damage to probiotic cells, and at the same time achieve efficient gas-liquid mixing and oxygen dissolution. Summary of the Invention

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: an automated propagation device for microbial agents, including a fermenter, a support component fixedly installed inside the fermenter, and a stirring mechanism connected to the support component, the stirring mechanism being used to stir and dissolve oxygen in the bacterial liquid inside the fermenter.

[0007] The stirring mechanism includes a rotating shaft rotatably connected to a support member. Several sets of zipper-type blades are fixedly installed on the rotating shaft. Each set includes a stirring blade and a herringbone plate. An aeration block is connected to the back surface of the stirring blade. When the rotating shaft rotates, the stirring blade pushes the bacterial liquid upward and downward, and then the herringbone plate gathers the bacterial liquid to the back surface of the stirring blade.

[0008] The stirring paddle has a rectangular channel inside, and several partitions are connected at equal intervals inside the channel. The lower side of the partition has a grooved air chamber, and a breathable membrane is detachably connected to the lower side of the partition. When the rotating shaft rotates, the bacterial liquid flows into the rectangular channel and is separated into several thin liquids by the partitions. Then these thin liquids come into contact with the breathable membranes at the corresponding positions, so that the oxygen in the grooved air chambers can permeate into the thin liquids through the breathable membranes.

[0009] The stirring mechanism also includes an air distribution component, which is used to introduce oxygen into the grooved air chamber and the aeration block respectively.

[0010] Preferably, a synchronous motor is fixedly installed on the upper end of the support member, and the synchronous motor is connected to the rotating shaft through a belt drive structure.

[0011] Preferably, the belt drive structure includes a driving pulley fixedly mounted on the output shaft of a synchronous motor, a driven pulley fixedly mounted on the outside of the rotating shaft, and a belt wound around the outside of the driving pulley and the driven pulley.

[0012] Preferably, the thickness of the material-facing surface of the agitator is less than the thickness of its material-receiving surface, and the material-receiving surface of the agitator has a steeply sloping vertical structure.

[0013] Preferably, the edges of the stirring paddle and the figure-eight plate are both designed with rounded corners, and several equally spaced corrugated plates are fixedly installed on the back surface of the stirring paddle.

[0014] Preferably, the aeration block is connected to the stirring paddle by screws. When the rotating shaft rotates, the aeration block simultaneously introduces microbubbles into the rectangular through groove and the intervals of the corrugated plate.

[0015] Preferably, the partition is detachably and sealingly connected inside the rectangular through groove by plugging in, and a pressure film frame is detachably connected to the lower side of the partition by countersunk screws.

[0016] Preferably, the air distribution assembly includes two separate air channels located inside the agitator, which are respectively connected to the grooved air chamber and the aeration block.

[0017] Preferably, an air distribution cylinder is fixedly installed on the upper end of the rotating shaft. The air distribution cylinder is divided into upper and lower regions. The lower region of the air distribution cylinder is connected to the inside and outside through a rectangular groove on it. The upper and lower regions of the air distribution cylinder are respectively connected to various air passages through flexible hoses.

[0018] Preferably, a venting component is sealed and fixedly installed on the fermenter. The upper part of the venting component consists of two separately arranged tubular structures, and the lower part consists of a coaxially arranged tubular structure. The venting component is rotatably connected to the gas distribution cylinder in a sealed manner, and the two tubular structures of the venting component are respectively connected to the upper and lower regions of the gas distribution cylinder.

[0019] The beneficial effects of this invention are as follows: First, this invention uses a rotating shaft to drive several sets of zipper-type blades to rotate at low speed, so that the blades stir the bacterial liquid. At the same time, the stirring blades push the bacterial liquid upward and downward, and the aeration block discharges micro-oxygen bubbles to the back surface of the stirring blades. Then, the figure-eight plate gathers the bacterial liquid to the back surface of the stirring blades, thereby forcing the bacterial liquid and micro-oxygen bubbles to mix and dissolve. Under the premise of maintaining low speed operation, efficient gas-liquid mixing and oxygen dissolution are achieved, which meets the high oxygen uptake requirements of aerobic probiotics, while avoiding damage to the bacteria from high shear stress.

[0020] Second, the present invention uses a rectangular channel inside the stirring paddle to introduce bacterial liquid, and then uses several partitions connected at equal intervals inside the channel to separate the bacterial liquid entering the rectangular channel into several thin liquids. Then, these thin liquids come into contact with the corresponding air-permeable membranes, so that the oxygen in the groove air chambers can permeate into the thin liquids through the air-permeable membranes and dissolve in the thin liquids, which greatly increases the gas-liquid contact area and mass transfer path, and significantly improves the dissolution efficiency of oxygen and bacterial liquid.

[0021] Third, the present invention uses several equally spaced corrugated plates fixedly installed on the back surface of the stirring paddle to extend the movement path of the micro-oxygen bubbles discharged from the aeration block in the back surface area, thereby extending the contact time between the micro-oxygen bubbles and the bacterial solution, so that these micro-oxygen bubbles can be more fully dissolved in the bacterial solution gathered by the figure-eight plates, further improving the oxygen utilization rate.

[0022] Fourth, the present invention adopts a rounded corner design at the edge of the stirring paddle and the figure-eight plate, which makes the direction change of the bacterial liquid smooth when flowing at low speed, avoiding the generation of local high shear zones. At the same time, combined with the overall low speed operation, it effectively reduces the shear damage to the long axis of the rod-shaped probiotics, ensuring the viability rate and spore formation rate. Attached Figure Description

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a partial cross-sectional view of the present invention; Figure 3 This is a partial sectional view of the rotating shaft, stirring paddle, figure-eight plate, and support components in this invention; Figure 4 This is a partial cross-sectional view of the ventilation component, air distribution cylinder, rotating shaft, and hose in this invention; Figure 5 This is a partial cross-sectional view of the stirring paddle, baffle, rotating shaft and figure-eight plate in this invention; Figure 6 This is a partial structural diagram of the figure-eight plate, stirring paddle, wave plate, and rotating shaft in this invention; Figure 7This is a schematic diagram of the structure of the stirring paddle, aeration block and corrugated plate in this invention; Figure 8 This is a partial cross-sectional view of the stirring paddle, air passage, baffle and aeration block in this invention; Figure 9 This is a partial cross-sectional view of the stirring paddle, air passage, and baffle in this invention; Figure 10 This is a partial cross-sectional view of the breathable membrane, the grooved air chamber, the partition, and the pressing frame in this invention.

[0025] In the diagram: 1. Fermentation tank; 2. Support component; 3. Stirring mechanism; 21. Synchronous motor; 22. Driving pulley; 23. Driven pulley; 31. Rotating shaft; 32. Stirring paddle; 33. Herringbone plate; 34. Baffle plate; 35. Breathable membrane; 36. Gas distribution assembly; 37. Aeration block; 321. Corrugated plate; 341. Grooved air chamber; 342. Pressing frame; 361. Air duct; 362. Air distributor; 363. Hose; 364. Ventilation component. Detailed Implementation

[0026] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the embodiments, they shall be performed in accordance with the techniques or conditions described in the literature in the art or in accordance with the product manual.

[0027] See Figure 1 , Figure 2 and Figure 3 An automated propagation device for microbial agents includes a fermenter 1, a support 2 fixedly installed inside the fermenter 1, and a stirring mechanism 3 connected to the support 2. The stirring mechanism 3 is used to stir and dissolve oxygen in the bacterial liquid inside the fermenter 1.

[0028] When propagating Bacillus subtilis, the prepared bacterial solution is placed in fermenter 1, and the bacterial solution in fermenter 1 is stirred at low speed by stirring mechanism 3. At the same time, oxygen is discharged into the bacterial solution. On the one hand, stirring increases the mixing effect between oxygen and bacterial solution, thereby improving oxygen dissolution efficiency. On the other hand, stirring mechanism 3 directly discharges tiny oxygen bubbles into bacterial solution, which can prolong the movement path of tiny oxygen bubbles in bacterial solution and force the bacterial solution to combine with tiny oxygen bubbles, further improving the oxygen dissolution efficiency of bacterial solution.

[0029] See Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 7 and Figure 10The stirring mechanism 3 includes a rotating shaft 31 rotatably connected to the support member 2. Several sets of zipper-type blades are fixedly installed on the rotating shaft 31. Each set includes a stirring blade 32 and a herringbone plate 33. An aeration block 37 is connected to the back surface of the stirring blade 32. When the rotating shaft 31 rotates, the stirring blade 32 pushes the bacterial liquid upward and downward. Then, the herringbone plate 33 gathers the bacterial liquid to the back surface of the stirring blade 32. The stirring mechanism 3 also includes an air distribution component 36, which is used to introduce oxygen into the grooved air chamber 341 and the aeration block 37 respectively.

[0030] When propagating Bacillus subtilis, the rotating shaft 31 is rotated at a low speed (less than 100 rpm). The rotating shaft 31 drives the stirring paddle 32 and the figure-eight plate 33 to rotate synchronously, so that the stirring paddle 32 and the figure-eight plate 33 stir the bacterial solution at a low speed. At the same time, the air distribution component 36 continuously introduces oxygen into the aeration block 37, so that the aeration block 37 continuously discharges tiny bubbles into the back surface of the stirring paddle 32, so that the bacterial solution gathered by the figure-eight plate 33 is forced to mix with these discharged tiny bubbles, thereby increasing the oxygen content in the bacterial solution.

[0031] See Figure 3 , Figure 5 , Figure 8 , Figure 9 and Figure 10 The stirring paddle 32 has a rectangular through groove inside, and several partitions 34 are connected at equal intervals inside the through groove. The lower side of the partition 34 has a grooved air chamber 341. A breathable membrane 35 is detachably connected to the lower side of the partition 34. When the rotating shaft 31 rotates, the bacterial liquid flows into the rectangular through groove and is separated into several thin liquids by the partitions 34. Then these thin liquids come into contact with the breathable membrane 35 at the corresponding positions.

[0032] The gas distribution component 36 continuously introduces oxygen into the grooved gas chamber 341, resulting in a higher oxygen pressure within the grooved gas chamber 341. This allows the oxygen in the grooved gas chamber 341 to permeate through the breathable membrane 35 and dissolve in the thin liquid, further increasing the oxygen content in the bacterial solution. Furthermore, by dividing the bacterial solution into several portions, each portion is in contact with the breathable membrane 35 in the corresponding partition 34, thus increasing the overall contact surface area between the bacterial solution and oxygen, thereby improving the dissolution efficiency of oxygen in the bacterial solution.

[0033] To facilitate low-speed rotation of the rotating shaft 31, the present invention designs the following structure: (See attached diagram) Figure 1 , Figure 2 and Figure 3 A synchronous motor 21 is fixedly installed on the upper end of the support member 2. The synchronous motor 21 is connected to the rotating shaft 31 through a belt drive structure. The belt drive structure includes a driving pulley 22 fixedly installed on the output shaft of the synchronous motor 21, a driven pulley 23 fixedly installed on the outside of the rotating shaft 31, and a belt wound around the outside of the driving pulley 22 and the driven pulley 23.

[0034] When propagating the bacterial agent, the synchronous motor 21 is started to rotate at low speed. The synchronous motor 21 drives the drive pulley 22 to rotate synchronously. The drive pulley 22 drives the driven pulley 23 to rotate through the belt. The driven pulley 23 drives the rotating shaft 31 to rotate, so that the rotating shaft 31 drives the stirring paddle 32 and the figure-eight plate 33 to stir the bacterial liquid.

[0035] It should be noted that the support 2 is sealed and fixedly connected to the fermenter 1, and the outer wall of the rotating shaft 31 is sealed and rotatably connected to the support 2, thereby ensuring the airtightness of the inside of the fermenter 1 when the inoculant is propagated.

[0036] To facilitate the efficient mixing of tiny oxygen bubbles by combining the stirring paddle 32 and the figure-eight plate 33 in a zipper-like arrangement, the present invention designs the following structure: (See reference) Figure 5 and Figure 6 The thickness of the material-facing surface of the agitator 32 is less than the thickness of its material-receiving surface, and the material-receiving surface of the agitator 32 has a steeply sloping vertical surface structure.

[0037] When the agitator 32 rotates, the bacterial solution moves along the upper and lower side walls of the agitator 32, causing the upper and lower side walls of the agitator 32 to push the bacterial solution in the upward and downward direction as they move. When these pushed bacterial solutions move to the back surface of the agitator 32, they simultaneously lose the obstruction of the agitator 32. At the same time, these bacterial solutions are pushed and gathered by the inner side of the herringbone plate 33, forcibly pushing them to the back surface of the agitator 32. This forces the bacterial solutions to mix with the tiny oxygen bubbles discharged from the back surface of the agitator 32. Subsequently, these bacterial solutions pass through the inner side of the herringbone plate 33.

[0038] In this embodiment, the figure-eight plate 33 is composed of a lower side plate, an upper side plate and a connecting arm. The lower side plate and the upper side plate are symmetrically arranged on the back surface of the stirring paddle 32, and the lower side plate and the upper side plate are combined to form a figure-eight structure. The connecting arm is fixed on the lower side plate and the upper side plate together, and the connecting arm is fixedly connected to the rotating shaft 31, so that the figure-eight plate 33 and the rotating shaft 31 rotate synchronously.

[0039] To reduce the damage rate to Bacillus subtilis during the rotation of the stirring paddle 32 and the figure-eight plate 33, and to increase the residence time of micro-oxygen bubbles in the bacterial solution, the present invention designs the following structure: (See reference) Figure 3 , Figure 5 , Figure 6 and Figure 7 The edges of the stirring paddle 32 and the figure-eight plate 33 are both designed with rounded corners. Several equally spaced wave plates 321 are fixedly installed on the back surface of the stirring paddle 32.

[0040] When the agitator 32 rotates, it drives the corrugated plate 321 on its back surface to rotate synchronously, and the aeration block 37 discharges micro-oxygen bubbles between two adjacent corrugated plates 321. This allows the corrugated plate 321 to push the micro-oxygen bubbles back and forth through its side, extending the movement path of the micro-oxygen bubbles discharged by the aeration block 37 in the back surface area. This extends the contact time between the micro-oxygen bubbles and the bacterial solution, allowing these micro-oxygen bubbles to dissolve more fully in the bacterial solution gathered by the figure-eight plate 33, further improving the oxygen utilization rate.

[0041] The rounded corners at the edges of the stirring paddle 32 and the figure-eight plate 33 ensure a smooth change in the direction of the bacterial solution when it flows at low speed, avoiding the formation of local high shear zones. Combined with the overall low-speed operation, this effectively reduces shear damage to the long axis of the rod-shaped probiotics, ensuring the viability and spore formation rate.

[0042] To facilitate the cleaning and replacement of the aeration block 37, the present invention is designed with the following structure: (See reference) Figure 7 and Figure 8 The aeration block 37 is connected to the stirring paddle 32 by screws, which makes it easy to disassemble the aeration block 37. When the rotating shaft 31 rotates, the aeration block 37 simultaneously introduces microbubbles into the rectangular through groove and the intervals of the wave plate 321.

[0043] To facilitate the disassembly and cleaning of the partition 34 and the replacement of the breathable membrane 35, the present invention is designed with the following structure: (See attached diagram) Figure 5 , Figure 8 , Figure 9 and Figure 10 The partition 34 is detachably and sealed inside the rectangular through groove by plugging in, and the pressure film frame 342 is detachably connected to the lower side of the partition 34 by countersunk screws.

[0044] When cleaning the partition 34, unscrew the screws that fix the partition 34 and pull the partition 34 out from the agitator 32 to clean the partition 34. When replacing the breathable membrane 35, unscrew the countersunk screws, remove the membrane frame 342 to separate the old breathable membrane 35 from the partition 34, then lay the new breathable membrane 35 flat on the grooved air chamber 341 of the partition 34, install the membrane frame 342, and lock the membrane frame 342 with the countersunk screws so that the membrane frame 342 presses the four sides of the breathable membrane 35 tightly onto the partition 34.

[0045] In this embodiment, oxygen is introduced into the grooved air chamber 341, causing the oxygen to push the breathable membrane 35 downwards, thereby bringing the breathable membrane 35 into contact with the bacterial liquid. Subsequently, the high-pressure oxygen in the grooved air chamber 341 passes through the breathable membrane 35 and dissolves in the bacterial liquid.

[0046] To facilitate the introduction of oxygen into the recessed air chamber 341 and the aeration block 37, the present invention is designed with the following structure: (See reference) Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 7 , Figure 8 and Figure 9 The air distribution component 36 includes two separate air passages 361 located inside the agitator 32. The two air passages 361 are respectively connected to the recessed air chamber 341 and the aeration block 37.

[0047] Continue reading Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 7 , Figure 8 and Figure 9 An air distribution cylinder 362 is fixedly installed on the upper end of the rotating shaft 31. The air distribution cylinder 362 is divided into upper and lower regions. The lower region of the air distribution cylinder 362 is connected to the inside and outside through a rectangular groove on it. The upper and lower regions of the air distribution cylinder 362 are respectively connected to each air passage 361 through a hose 363.

[0048] Continue reading Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 7 , Figure 8 and Figure 9 A venting component 364 is sealed and fixedly installed on the fermenter 1. The upper part of the venting component 364 consists of two separately arranged tubular structures, and the lower part consists of a coaxially arranged tubular structure. The venting component 364 is rotatably connected to the gas distribution cylinder 362 in a sealed manner, and the two tubular structures of the venting component 364 are respectively connected to the upper and lower areas of the gas distribution cylinder 362.

[0049] The two tubular structures on the upper part of the ventilation component 364 are connected to two oxygen supply devices respectively. When the bacterial agent is propagated, the two oxygen supply devices respectively introduce oxygen into the two tubular structures on the upper part of the ventilation component 364 at a specified flow rate and pressure. The oxygen in one of the tubular structures flows directly to the upper part of the air distribution cylinder 362, and then flows along the corresponding hose 363 and air passage 361 to the aeration block 37, so that the aeration block 37 refines the oxygen into tiny bubbles and discharges them into the bacterial liquid.

[0050] Oxygen from another tubular structure flows to the lower section of the gas distribution cylinder 362, and then flows along the corresponding hose 363 and air passage 361 to each inner recessed air chamber 341, thereby allowing the oxygen in the recessed air chamber 341 to pass through the breathable membrane 35 and dissolve in the bacterial solution.

[0051] It should be noted that, in order to achieve industrialized manufacturing and convenient maintenance of the present invention, the stirring paddle 32 and the rotating shaft 31 can be manufactured by separate casting and then welding: the stirring paddle 32 and the rotating shaft 31 are cast separately and then fixedly connected as one unit by welding to ensure the alignment and sealing of the internal air passage 361; the corrugated plate 321 and the herringbone plate 33 are respectively installed on the corresponding positions of the rotating shaft 31 and the stirring paddle 32 by welding, which is convenient for later disassembly and cleaning; each partition 34 is cast separately, and after surface polishing and precision treatment, it is detachably and sealed to the inside of the rectangular through groove of the stirring paddle 32 by plugging to ensure the flatness and airtightness of the permeable membrane 35. The above manufacturing and assembly methods can effectively reduce the processing difficulty, ensure the fitting accuracy of each component, and facilitate cleaning, replacement and maintenance during use, and have good industrial practicality and operability.

[0052] It is worth noting that, compared with traditional fermentation and propagation devices, although the present invention adds components such as the stirring paddle 32, the figure-eight plate 33, the aeration block 37, and the breathable membrane 35 to the structure, slightly increasing the initial equipment investment cost, the synergistic effect of the above structures can effectively improve the oxygen dissolution rate while maintaining low-speed operation, effectively reduce the shear damage to rod-shaped probiotics such as Bacillus subtilis, significantly improve the viability and spore formation rate, and shorten the propagation cycle while reducing energy consumption and operating costs. Therefore, the present invention can quickly balance the initial investment and achieve higher output returns, and has good economic practicality and market promotion value.

[0053] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0054] Furthermore, the terms "first," "second," "number one," and "number two" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first," "second," "number one," or "number two" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0055] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0056] The embodiments described herein are preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. An automated microbial inoculant propagation device, comprising a fermenter, characterized in that, The fermenter is equipped with a support structure, and a stirring mechanism is connected to the support structure. The stirring mechanism is used to stir and dissolve oxygen in the bacterial solution inside the fermenter. The stirring mechanism includes a rotating shaft rotatably connected to a support member. Several sets of zipper-type blades are fixedly installed on the rotating shaft. Each set includes a stirring blade and a herringbone plate. An aeration block is connected to the back surface of the stirring blade. When the rotating shaft rotates, the stirring blade pushes the bacterial liquid upward and downward, and then the herringbone plate gathers the bacterial liquid to the back surface of the stirring blade. The stirring paddle has a rectangular through groove inside, and several partitions are connected at equal intervals inside the through groove. A grooved air chamber is opened on the lower side of the partition. A breathable membrane is detachably connected to the lower side of the partition. When the rotating shaft rotates, the bacterial liquid flows into the rectangular through groove and is separated into several thin liquids by the partitions. Then these thin liquids come into contact with the breathable membranes at the corresponding positions, so that the oxygen in the grooved air chambers can penetrate into the thin liquids through the breathable membranes and dissolve in the thin liquids. The stirring mechanism also includes an air distribution component, which is used to introduce oxygen into the grooved air chamber and the aeration block respectively.

2. The automated microbial agent propagation device according to claim 1, characterized in that, A synchronous motor is fixedly installed on the upper end of the support member, and the synchronous motor is connected to the rotating shaft through a belt drive structure.

3. The automated microbial agent propagation device according to claim 2, characterized in that, The belt drive structure includes a driving pulley fixedly mounted on the output shaft of a synchronous motor, a driven pulley fixedly mounted on the outside of a rotating shaft, and a belt wound around the outside of the driving pulley and the driven pulley.

4. The automated microbial agent propagation device according to claim 1, characterized in that, The thickness of the material-facing surface of the agitator is less than the thickness of its material-receiving surface, and the material-receiving surface of the agitator has a steeply sloping vertical structure.

5. The automated microbial agent propagation device according to claim 1, characterized in that, The edges of both the stirring paddle and the figure-eight plate are designed with rounded corners, and several equally spaced wave plates are fixedly installed on the back surface of the stirring paddle.

6. The automated microbial agent propagation device according to claim 5, characterized in that, The aeration block is connected to the stirring paddle by screws. When the rotating shaft rotates, the aeration block simultaneously introduces microbubbles into the rectangular through groove and the intervals of the corrugated plate.

7. The automated microbial agent propagation device according to claim 5, characterized in that, The partition is detachably and sealed inside the rectangular through groove by plugging in, and a pressure film frame is detachably connected to the lower side of the partition by countersunk screws.

8. The automated microbial agent propagation device according to claim 1, characterized in that, The air distribution assembly includes two separate air channels located inside the agitator, which are connected to the grooved air chamber and the aeration block, respectively.

9. The automated microbial agent propagation device according to claim 8, characterized in that, An air distribution cylinder is fixedly installed on the upper end of the rotating shaft. The air distribution cylinder is divided into upper and lower regions. The lower region of the air distribution cylinder is connected to the inside and outside through a rectangular groove on it. The upper and lower regions of the air distribution cylinder are connected to various air passages through flexible hoses.

10. The automated microbial agent propagation device according to claim 9, characterized in that, The fermenter is sealed and fixedly installed with a venting component. The upper part of the venting component consists of two separately arranged tubular structures, and the lower part consists of a coaxially arranged tubular structure. The venting component is rotatably connected to the gas distribution cylinder in a sealed manner, and the two tubular structures of the venting component are respectively connected to the upper and lower areas of the gas distribution cylinder.

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