A deep-sea high-pressure isobaric culture device

Abstract

This invention discloses a deep-sea high-pressure isobaric culture device, belonging to the field of embryo culture and deep-sea extreme environment life research technology. The device includes a culture unit and a gas source unit. The culture unit consists of a storage tank, a culture chamber, and a waste tank connected from top to bottom via ball valves. Each chamber is equipped with a gas connection valve and an overflow valve. The gas source unit simultaneously introduces a controllable mixture of gases into the storage tank, culture chamber, and waste tank via gas cylinders and pressure reducing valves, maintaining a uniform and stable high-pressure environment in all three chambers. This invention enables isobaric and stable culture exchange without depressurization or opening of the chambers, through ball valve control, allowing the old culture medium in the culture chamber to be discharged into the waste tank and the new culture medium to be replenished from the storage tank. This effectively solves the problems of pressure fluctuations, sample damage, and poor experimental repeatability in traditional high-pressure culture devices, and is suitable for the continuous steady-state culture requirements of embryos under deep-sea high-pressure simulation.

Description

Technical Field

[0001] This invention belongs to the field of embryo culture technology, and specifically relates to a deep-sea high-pressure isobaric culture device. Background Technology

[0002] In life science research in extreme deep-sea environments, to simulate the high-pressure environment and conduct long-term biological culture, it is necessary to replace the culture medium while maintaining stable pressure within the chamber to ensure normal sample growth and development. Most existing high-pressure culture devices employ a single-chamber, closed structure, capable only of pressurizing, maintaining, and depressurizing, which is insufficient to meet the experimental requirements of dynamic medium replacement.

[0003] Traditional devices require a process of depressurization, chamber opening, medium replacement, chamber closure, and repressurization when changing the culture medium. This process causes drastic pressure fluctuations, disrupts the steady state of the culture environment, and can easily cause physical damage to biological samples. Furthermore, the devices lack an integrated structure for independent storage, culture, and waste collection, resulting in unstable pressure control and significant fluid impact during medium replacement, making it impossible to achieve stable, undisturbed isobaric medium replacement.

[0004] Existing equipment cannot smoothly replace the culture medium under pressure, resulting in cumbersome procedures, poor experimental repeatability, and difficulty in supporting continuous culture research under high-pressure deep-sea environments. Therefore, developing a multi-chamber culture device capable of stable medium replacement under isobaric conditions has become an urgent technical problem to be solved in this field. Summary of the Invention

[0005] To address the aforementioned problems, the purpose of this invention is to provide a deep-sea high-pressure isobaric culture device.

[0006] The technical solution of the present invention is: a deep-sea high-pressure isobaric liquid exchange culture device, comprising a culture unit and a gas source unit.

[0007] The culture unit includes a storage tank, a culture chamber, and a waste tank connected sequentially from top to bottom via ball valves. The culture chamber is used to hold petri dishes and a first culture medium, while the storage tank is used to hold a second culture medium. Each of the storage tank, culture chamber, and waste tank is equipped with a gas connection valve and an overflow valve. After the first culture stage is completed, the ball valve between the culture chamber and the waste tank is opened, and the first culture medium in the culture chamber is discharged into the waste tank. When the first culture medium has drained out, the ball valve between the culture chamber and the waste tank is closed, and the ball valve between the storage tank and the culture chamber is opened, allowing the second culture medium in the storage tank to be discharged into the culture chamber. When the second culture medium fills the culture chamber, the ball valve between the storage tank and the culture chamber is closed, and the second culture stage begins.

[0008] The gas source unit stores a mixed gas and is connected to the three gas connection valves respectively, for introducing the mixed gas into the storage tank, culture tank and waste tank to maintain a uniform and stable pressure environment inside the storage tank, culture tank and waste tank.

[0009] Furthermore, the gas source unit includes a gas cylinder and a pressure reducing valve. The gas cylinder stores a mixed gas inside. The pressure reducing valve has an inlet end and an outlet end. The inlet end is connected to the gas cylinder, and the outlet end is connected to three gas connection valves respectively, which are used to depressurize the mixed gas and introduce it into the liquid storage tank, the culture tank and the waste liquid tank respectively.

[0010] Furthermore, the pressure of the mixed gas inside the cylinder is 6MPa to 8MPa.

[0011] Furthermore, the mixed gas is a mixture of carbon dioxide, oxygen, and nitrogen in a volume ratio of 1:1:(16-20).

[0012] Furthermore, the pressure of the mixed gas is 7 MPa.

[0013] Furthermore, the storage tank, culture tank, and waste tank have identical structures, each having four interfaces; one interface of the storage tank is connected to one end of a ball valve, and the other end of the ball valve is connected to one interface of the culture tank; another interface of the culture tank is connected to one end of another ball valve, and the other end of the ball valve is connected to one interface of the waste tank; three gas connection valves are installed one-to-one on one interface of the storage tank, one interface of the culture tank, and one interface of the waste tank; three overflow valves are installed one-to-one on one interface of the storage tank, one interface of the culture tank, and one interface of the waste tank; the last interface of the storage tank and the last interface of the waste tank are both equipped with plugs.

[0014] Furthermore, the four interfaces are arranged in a cross-shaped structure.

[0015] Furthermore, the culture chamber is also equipped with two filter membranes, which are respectively installed at the interface connecting the culture chamber and the storage tank, and at the interface connecting the culture chamber and the waste tank.

[0016] Furthermore, the interface is connected to the ball valve, gas connection valve, relief valve, and plug via threads.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention uses a storage tank, a culture tank, and a waste tank arranged in sequence, and uses interfaces, ball valves, overflow valves, gas connection valves, and plugs to form a standardized connection structure. With the help of a gas source unit, mixed gas is simultaneously introduced into the storage tank, culture tank, and waste tank, so that the internal pressure of the storage tank, culture tank, and waste tank is kept consistent. Isobaric liquid exchange can be achieved without depressurization or opening of the tank. At the same time, through the functional zoning of the culture tank, storage tank, and waste tank and the on / off control of the ball valve, a closed and stable flow path for storage, culture, and waste liquid collection is formed. This avoids pressure fluctuations and fluid impacts during the liquid exchange process and achieves precise and stable pressure control. The overall structure is simple, easy to assemble, and reliable in sealing, which can significantly improve the stability, safety, and experimental repeatability of embryo culture under deep-sea high-pressure environment. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a partial structural schematic diagram of the present invention.

[0019] Wherein, 1—culture unit; 10—ball valve; 100—interface; 11—liquid storage tank; 12—culture chamber; 13—waste liquid tank; 14—gas connection valve; 15—overflow valve; 16—plug; 17—filter membrane; 2—gas source unit; 21—gas cylinder; 22—pressure reducing valve. Detailed Implementation

[0020] The following is combined Figure 1 , Figure 2 The specific embodiments of the present invention will be described in detail below. In the description of the present invention, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0021] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature; in the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0022] Example like Figure 1 The deep-sea high-pressure isobaric culture device shown includes a culture unit 1 and a gas source unit 2.

[0023] The culture unit 1 includes a storage tank 11, a culture tank 12, and a waste tank 13 connected sequentially from top to bottom by a ball valve 10. The culture tank 12 is used to hold culture dishes and a first culture medium, while the storage tank 11 is used to hold a second culture medium. Each of the storage tank 11, culture tank 12, and waste tank 13 is equipped with a gas connection valve 14 and an overflow valve 15. After the first culture stage is completed, the ball valve 10 between the culture tank 12 and the waste tank 13 is opened, and the first culture medium in the culture tank 12 is discharged into the waste tank 13. When the first culture medium has drained out, the ball valve 10 between the culture tank 12 and the waste tank 13 is closed, and the ball valve 10 between the storage tank 11 and the culture tank 12 is opened, so that the second culture medium in the storage tank 11 is discharged into the culture tank 12. When the second culture medium fills the culture tank 12, the ball valve 10 between the storage tank 11 and the culture tank 12 is closed, and the second culture stage begins.

[0024] The gas source unit 2 stores mixed gas and is connected to three gas connection valves 14 respectively, which are used to introduce mixed gas into the liquid storage tank 11, the culture tank 12 and the waste liquid tank 13 to maintain a uniform and stable pressure environment inside the liquid storage tank 11, the culture tank 12 and the waste liquid tank 13.

[0025] Preferably, the gas source unit 2 includes a gas cylinder 21 and a pressure reducing valve 22. The gas cylinder 21 stores a mixed gas. The pressure reducing valve 22 has an inlet end and an outlet end. The inlet end is connected to the gas cylinder 21, and the outlet end is connected to three gas connection valves 14 respectively, used to depressurize the mixed gas and introduce it into the liquid storage tank 11, the culture tank 12, and the waste liquid tank 13 respectively. This structure can achieve synchronous gas supply to multiple tanks at the same pressure with a single gas source. From the gas supply principle, it ensures that the pressure of the liquid storage tank 11, the culture tank 12, and the waste liquid tank 13 is always consistent, avoiding pressure difference, liquid backflow, or fluid impact problems caused by uneven gas inlet pressure in each tank.

[0026] Preferably, the pressure of the mixed gas inside the gas cylinder 21 is 6MPa to 8MPa. The gas cylinder 21 provides a high-pressure reserve gas source of 6MPa to 8MPa, which can meet the pressure supply requirements of deep-sea high-pressure simulation. Combined with the precise pressure reduction control of the pressure reducing valve 22, the output pressure can be stabilized within the safe operating range required for cultivation, achieving stable pressure establishment and continuous maintenance. Simultaneously, the pressure reducing valve 22 adopts a single-input, multi-parallel-output connection method, directly and evenly distributing gas to the three gas connection valves 14. This ensures pressure balance within the storage tank 11, culture tank 12, and waste tank 13 without the need for additional distribution devices. This simplifies the gas path structure, improves pressure control accuracy, and maintains a stable pressure throughout the liquid exchange process, avoiding damage to the embryos caused by pressure fluctuations, significantly improving the stability and reliability of the high-pressure culture system. Preferably, in this embodiment, the pressure of the mixed gas is 7MPa.

[0027] Preferably, the mixed gas is a mixture of carbon dioxide, oxygen, and nitrogen in a volume ratio of 1:1:(16-20). In this embodiment, the preferred volume ratio is 1:1:18. The mixed gas is simultaneously introduced into the storage tank 11, culture tank 12, and waste tank 13 via gas cylinder 21, pressure reducing valve 22, and gas connection valve 14 to provide a stable gaseous environment for embryo culture. From the perspective of the physiological requirements of embryo culture and the principle of maintaining high pressure stability, this ratio can form a gas partial pressure system in the storage tank 11, culture tank 12, and waste tank 13 that is most suitable for the in vitro development of early embryos. The oxygen component meets the energy requirements of aerobic metabolism of the embryo, the carbon dioxide component is used to stabilize the pH of the culture medium and maintain the physiological osmotic pressure required for embryo development, and nitrogen, as an inert balancing gas, does not participate in cell biochemical reactions and is only used to stabilize the total gas pressure and avoid fluctuations in the proportion of other gases. The 1:1:(16-20) ratio of the three components can make the partial pressure of each component highly suitable for the physiological standards of in vitro embryo culture. In the deep-sea high-pressure simulation scenario, the mixed gas with this ratio is pressurized by the pressure reducing valve 22 and then introduced into the storage tank 11, culture tank 12, and waste tank 13. This ensures that the gas phase composition remains stable throughout the isobaric liquid exchange process, without sudden changes in proportion due to culture medium replacement or fluid flow. Combined with the venting and pressure stabilization function of the overflow valve 15, this further guarantees the dual stability of the gas phase and pressure in the culture environment. Simultaneously, this ratio is highly versatile and adaptable to the culture requirements of mammalian embryos and human waste research embryos. It can operate continuously and stably in a closed flow path controlled by the interface 100 and ball valve 10. This avoids embryonic developmental arrest or culture medium shock caused by gas phase imbalance and works in synergy with the device's isobaric liquid exchange mechanism to improve the survival rate and developmental stability of embryos under high-pressure extreme environments.

[0028] Preferably, the storage tank 11, culture tank 12, and waste tank 13 have the same structure and each has four ports 100; one port 100 of the storage tank 11 is connected to one end of a ball valve 10, and the other end of the ball valve 10 is connected to one port 100 of the culture tank 12; another port 100 of the culture tank 12 is connected to one end of another ball valve 10, and the other end of the ball valve 10 is connected to one port 100 of the waste tank 13; three gas connection valves 14 are installed one-to-one on one port 100 of the storage tank 11, one port 100 of the culture tank 12, and one port 100 of the waste tank 13; three overflow valves 15 are installed one-to-one on one port 100 of the storage tank 11, one port 100 of the culture tank 12, and one port 100 of the waste tank 13; the last port 100 of the storage tank 11 and the last port 100 of the waste tank 13 are each provided with a plug 16.

[0029] It should be noted that an adapter is installed on the interface 100 connecting the liquid storage tank 11 to the plug 16. The plug 16 is installed on the adapter. The purpose of the adapter is to facilitate the addition of culture medium. When it is necessary to add culture medium to the liquid storage tank 11, the plug 16 is removed from the adapter and the culture medium is added to the liquid storage tank 11 through the adapter.

[0030] Preferably, the four interfaces 100 are arranged in a cross structure. This cross-shaped arrangement ensures that the interfaces 100 are centrally symmetrical and evenly positioned, providing non-interfering installation positions for the ball valve 10, gas connection valve 14, overflow valve 15, and plug 16. From a structural assembly perspective, the cross layout allows the liquid storage tank 11, culture tank 12, and waste liquid tank 13 to maintain coaxial straight-line communication when connected to the ball valve 10 via the interfaces 100. This ensures smooth straight-line flow of the culture medium among the liquid storage tank 11, culture tank 12, and waste liquid tank 13, avoiding localized impacts caused by increased fluid resistance or uneven fluid exchange flow rates. In addition, the cross-shaped interface layout has a high degree of standardization and versatility. The storage tank 11, culture tank 12, and waste tank 13 of the same structure can be used interchangeably without the need for separate special tanks. This simplifies the processing and assembly process, reduces manufacturing costs, and facilitates quick connection and sealing of valves and pipelines. From the structural design level, it improves the overall stability, ease of operation, and interchangeability of the device, providing reliable structural support for isobaric liquid replacement.

[0031] Preferred, such as Figure 2 As shown, the culture chamber 12 is also equipped with filter membranes 17. There are two filter membranes 17, which are correspondingly installed at the interfaces connecting the culture chamber 12 and the storage chamber 11, and at the interfaces connecting the culture chamber 12 and the waste liquid chamber 13. The filter membranes 17 are mainly used to prevent the embryos in the culture chamber 12 from being discharged from the interface 100 of the culture chamber 12 during medium replacement. When the ball valve 10 is opened and the culture medium flows between the storage chamber 11, the culture chamber 12, and the waste liquid chamber 13 under pressure, the filter membrane 17 in the storage chamber 11 can form a bidirectional interception barrier, allowing the liquid to pass freely while stably retaining the embryos in the culture chamber 12, completely preventing the embryos from entering the interface 100, pipeline, or ball valve 10 with the liquid flow, causing loss, jamming, or damage. This structure, combined with the isobaric gas supply and the pressure stabilization mechanism of the overflow valve 15, can achieve in-situ fixed culture of embryos without affecting the smooth replacement of the culture medium, ensuring that there is no sample loss or physical pulling during the entire medium replacement process. In addition, the dual-sided filter membrane 17 layout enables the device to effectively intercept liquids whether the liquid is changed in the forward direction or replenished in the reverse direction, which improves the operational flexibility and system fault tolerance. The structure ensures the safety of the high-pressure liquid change process and the success rate of experiments, and is especially suitable for the stringent culture requirements of trace and precious embryo samples.

[0032] Preferably, the interface 100 is connected to the ball valve 10, gas connection valve 14, relief valve 15, and plug 16 via threads. This connection method is highly standardized and easy to assemble and disassemble, requiring no special tools for assembly and maintenance. This allows for quick replacement or maintenance of the ball valve 10, relief valve 15, gas connection valve 14, and plug 16, improving the flexibility and efficiency of the device. The unified threaded connection also enables interchangeable parts, reducing processing and assembly difficulty and further improving the overall stability of the device.

[0033] In this embodiment, a storage chamber 11, a culture chamber 12, and a waste liquid chamber 13 are arranged in sequence, and the interface 100 of the storage chamber 11, culture chamber 12, and waste liquid chamber 13 are connected by a ball valve 10. In conjunction with the gas source unit 2, mixed gas is introduced into the storage chamber 11, culture chamber 12, and waste liquid chamber 13 to keep the pressure of the storage chamber 11, culture chamber 12, and waste liquid chamber 13 consistent. Isobaric liquid replacement is achieved without depressurization or opening of the chambers, and structurally avoids pressure fluctuations from damaging the sample.

[0034] By using the on / off control of ball valve 10, the first culture medium is discharged and the second culture medium is injected in a stepwise manner, forming an integrated closed flow path for liquid storage, culture and waste liquid collection. The liquid exchange process is undisturbed and without reverse impact, ensuring a continuous and stable culture environment.

[0035] The liquid storage chamber 11, culture chamber 12, and waste liquid chamber 13 are each independently equipped with a gas connection valve 14 and an overflow valve 15. The gas connection valve 14 allows for synchronous gas intake and pressure stabilization, while the overflow valve 15 discharges excess gas from the chamber. This structurally achieves pressure balance and real-time pressure stabilization in the three chambers, improving pressure control accuracy and experimental repeatability.

[0036] The liquid storage tank 11, culture tank 12, and waste liquid tank 13 have the same structure and are all equipped with four interfaces 100, which can be connected to ball valve 10, overflow valve 15, gas connection valve 14 and plug 16 respectively. The interface layout is uniform, the connection method is standardized, the structure is simple, the assembly is efficient, and the sealing is reliable, which simplifies the high-pressure culture operation process.

[0037] It should be noted that: such as Figure 1 As shown, in actual use, this culture device is used in conjunction with a constant temperature water bath. The culture unit 1 is immersed in the warm water of the constant temperature water bath so that the culture unit 1 is kept at the required temperature during the culture process.

[0038] A deep-sea high-pressure isobaric culture method, which uses the culture device proposed in this embodiment to culture embryos, includes the following steps: like Figure 1As shown, a culture dish containing embryos is placed in a culture chamber 12. A gas connection valve 14 is installed on the interface 100 on the left side of the culture chamber 12, and an overflow valve 15 is installed on the interface 100 on the right side. Ball valves 10 are connected to the upper and lower interfaces 100 respectively. The ball valve 10 of the lower interface 100 is closed, and the ball valve 10 of the upper interface 100 is opened. The culture chamber 12 is filled with the first culture medium using the ball valve 10 of the upper interface 100, and then the ball valve 10 is closed.

[0039] Install the lower interface of the storage tank 11 onto the other end of the ball valve 10 above the culture chamber 12, and install the upper interface of the waste liquid tank 13 onto the other end of the ball valve 10 below the culture chamber 12; install gas connection valves 14 on the left interface 100 of the storage tank 11 and the left interface 100 of the waste liquid tank 13 respectively, and install overflow valves 15 on the right interface 100 of the storage tank 11 and the right interface 100 of the waste liquid tank 13 respectively; install a plug 16 on the lower interface 100 of the waste liquid tank 13, install an adapter on the upper interface of the storage tank 11, and fill the storage tank 11 with the second culture medium using the adapter, then install the plug 16 on the adapter.

[0040] Connect the gas cylinder 21 to the inlet end of the pressure reducing valve 22, and connect the outlet end of the pressure reducing valve 22 to the three gas connection valves 14 respectively. Continuously introduce mixed gas into the liquid storage tank 11, the culture tank 12, and the waste liquid tank 13, and continue to culture until the embryo completes the initial cleavage.

[0041] Open the ball valve 10 between the culture chamber 12 and the waste liquid chamber 13 to allow the first culture medium to flow from the culture chamber 12 into the waste liquid chamber 13 until it flows out completely, then close the ball valve 10; open the ball valve 10 between the storage chamber 11 and the culture chamber 12 to allow the second culture medium to flow from the storage chamber 11 into the culture chamber 12 until it flows out completely, then close the ball valve 10, and continue culturing until the blastocyst stage is completed.

[0042] Preferably, the pressure reducing valve 22 reduces the pressure of the mixed gas to 0.8 MPa to 1.2 MPa before introducing it into the storage tank 11, culture tank 12, and waste tank 13. In this embodiment, the mixed gas is reduced to 1.0 MPa before being introduced into the storage tank 11, culture tank 12, and waste tank 13. This pressure range meets the pressure simulation requirements for life research in the extreme environment of the deep sea. At the same time, this pressure range is highly matched with the pressure-bearing and sealing performance of the ball valve 10, overflow valve 15, interface 100, and plug 16. It will not cause sealing failure, pipeline deformation, or excessive fluid impact due to excessive pressure, nor will it fail to achieve effective pressure maintenance and isobaric liquid exchange due to excessively low pressure. During the liquid exchange process, the pressure reducing valve 22 continuously and stably outputs a pressure of 0.8 MPa to 1.2 MPa, and in conjunction with the independently set overflow valve 15 of each tank, excess gas is discharged in real time, so that the storage tank 11, culture tank 12, and waste tank 13 always maintain pressure balance, avoiding embryo damage, culture medium backflow, or poor liquid exchange caused by pressure fluctuations or pressure differences. In addition, the pressure drop range is gradual and highly controllable, which can be perfectly adapted to the isobaric fluid exchange process, ensuring no sudden pressure changes and no fluid disturbances throughout the fluid exchange process, significantly improving the stability of the embryo culture environment and the reliability of experimental results.

[0043] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and do not limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention are included within the protection scope of the present invention.

Claims

1. A deep-sea high-pressure isobaric culture device, characterized in that, include: The culture unit includes a storage tank, a culture chamber, and a waste tank connected sequentially from top to bottom via ball valves. The culture chamber is used to hold petri dishes and a first culture medium, while the storage tank is used to hold a second culture medium. Each of the storage tank, culture chamber, and waste tank is equipped with a gas connection valve and an overflow valve. After the first culture stage is completed, the ball valve between the culture chamber and the waste tank is opened, and the first culture medium in the culture chamber is discharged into the waste tank. When the first culture medium has drained out, the ball valve between the culture chamber and the waste tank is closed, and the ball valve between the storage tank and the culture chamber is opened, allowing the second culture medium in the storage tank to be discharged into the culture chamber. When the second culture medium fills the culture chamber, the ball valve between the storage tank and the culture chamber is closed, and the second culture stage begins. The gas source unit stores a mixed gas and is connected to the three gas connection valves respectively, for introducing the mixed gas into the liquid storage tank, the culture tank and the waste liquid tank to maintain a uniform and stable pressure environment inside the liquid storage tank, the culture tank and the waste liquid tank.

2. The deep-sea high-pressure isobaric culture device as described in claim 1, characterized in that, The gas source unit includes: Gas cylinder, which stores a mixture of gases; The pressure reducing valve has an inlet end and an outlet end. The inlet end is connected to the gas cylinder, and the outlet end is connected to three gas connection valves respectively. It is used to depressurize the mixed gas and introduce it into the storage tank, the culture tank and the waste tank respectively.

3. The deep-sea high-pressure isobaric culture device as described in claim 2, characterized in that, The pressure of the mixed gas inside the gas cylinder is 6MPa to 8MPa.

4. The deep-sea high-pressure isobaric culture device as described in claim 3, characterized in that, The mixed gas is a mixture of carbon dioxide, oxygen, and nitrogen in a volume ratio of 1:1:(16-20).

5. The deep-sea high-pressure isobaric culture device as described in claim 3, characterized in that, The pressure of the mixed gas is 7 MPa.

6. The deep-sea high-pressure isobaric culture device as described in claim 1, characterized in that, The storage tank, culture tank and waste tank have the same structure and each has four interfaces; One of the interfaces of the liquid storage chamber is connected to one end of a ball valve, and the other end of the ball valve is connected to one of the interfaces of the culture chamber. The other port of the culture chamber is connected to one end of another ball valve, the other end of which is connected to one of the ports of the waste liquid tank; Three gas connection valves are installed one-to-one on one of the interfaces of the liquid storage tank, one of the interfaces of the culture tank, and one of the interfaces of the waste liquid tank. The three overflow valves are installed one-to-one on one of the interfaces of the storage tank, one of the interfaces of the culture tank, and one of the interfaces of the waste tank. Both the last port of the liquid storage tank and the last port of the waste liquid tank are equipped with plugs.

7. The deep-sea high-pressure isobaric culture device as described in claim 6, characterized in that, The four interfaces are arranged in a cross-shaped pattern.

8. The deep-sea high-pressure isobaric culture device as described in claim 6, characterized in that, The culture chamber is also equipped with two filter membranes, which are installed one-to-one at the interface connecting the culture chamber and the storage tank, and at the interface connecting the culture chamber and the waste tank.

9. The deep-sea high-pressure isobaric culture device as described in claim 6, characterized in that, The interface is connected to the ball valve, gas connection valve, relief valve, and plug via threads.

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