Working method of device for extracting and separating organic carbon components in kelp culture water body

Abstract

The application relates to a working method of an organic carbon component extraction and separation device for kelp culture water bodies, which comprises a first dimension of a grading extraction system and a second dimension of an elution and separation system; the first dimension of the grading extraction system comprises a sample supply module, a filter column, a first-stage extraction column and a second-stage extraction column, the output end of the sample supply module is sequentially connected with the filter column, the first-stage extraction column, the second-stage extraction column and a waste liquid collecting module along a liquid flow direction; and the second dimension of the elution and separation system comprises a first-stage elution module and a second-stage elution module. The application is based on the first dimension of the grading extraction system to continuously carry out particle carbon capture, inorganic carbonate removal and dissolved organic carbon extraction in the kelp culture water bodies, and the second dimension of the elution and separation system realizes the purging and elution of different polarity dissolved organic carbon substances, so that the continuous grading extraction and separation of hydrophilic and hydrophobic dissolved organic carbon in the kelp culture water can be realized.

Description

Technical Field

[0001] This invention relates to a working method for an extraction and separation device for organic carbon components in kelp aquaculture water, belonging to the field of environmental monitoring instruments and equipment. Background Technology

[0002] Analysis of the distribution and intensity of carbon sinks in marine algae aquaculture can provide scientific support for future measurement of marine algae carbon sinks. Besides the carbon sinks that can be removed from the aquaculture environment (i.e., harvested algae), algae carbon sinks also include dissolved organic carbon (DOC) pools (DOC) and inert dissolved organic carbon (RDOC) pools (RDOC) driven by microbial activity. Effective extraction and separation of DOC generated in kelp aquaculture is crucial for monitoring and evaluating the forms and content of DOC, and for assessing the organic carbon storage in kelp aquaculture waters.

[0003] Currently, in the analysis of dissolved organic carbon (DOC), existing technologies mainly focus on eutrophication assessment and source tracing. Chen et al. [Environmental Science & Technology, 2015, 148:3998] used parallel factor analysis (PARAFAC) to extract relevant information from three-dimensional fluorescence spectroscopy (EES) and combined it with principal component analysis to identify the impact of different DOC sources on eutrophication assessment. Wu et al. [Marine and Limnological Sciences, 2022, 1:319] studied the correlation between DOC and COD values ​​using three-dimensional fluorescence spectroscopy and conducted eutrophication assessment analysis of nearshore seawater. Guo et al. [Environmental Science, 2010, 143:2178] used the EES-PARAFAC method to analyze the sources and tracer analysis of DOM in estuarine water and the impact of DOC release from sediment on the eutrophication of overlying water bodies. Han et al. [Environmental Science, 2017, 38:2883] used macroporous adsorption resin to separate disinfection byproduct precursors in wastewater into hydrophilic, hydrophobic, and weakly hydrophobic substances. However, due to the high salinity and low organic matter content of seawater, extracting and separating dissolved organic carbon (DOC) from seawater used in kelp farming and analyzing its composition has been difficult. Therefore, it is necessary to establish a small-scale automated two-stage filtration, acidification, and extraction column separation device to develop a simple and practical continuous extraction technology for DOC in kelp farming water. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the technical problem to be solved by the present invention is to provide a working method for an extraction and separation device for organic carbon components in kelp aquaculture water.

[0005] To solve the above-mentioned technical problems, the technical solution of the present invention is: an apparatus for extracting and separating organic carbon components in kelp aquaculture water, comprising a first-dimensional graded extraction system and a second-dimensional elution separation system; the first-dimensional graded extraction system includes a sample supply module, a filter column, a first-stage extraction column, and a second-stage extraction column, wherein the output end of the sample supply module is sequentially connected to the filter column, the first-stage extraction column, the second-stage extraction column, and a waste liquid collection module along the liquid flow direction; the second-dimensional elution separation system includes a first-stage elution module and a second-stage elution module, wherein the output end of the first-stage elution module is connected between the filter column and the first-stage extraction column, and the output end of the second-stage elution module is connected between the first-stage extraction column and the second-stage extraction column; the output end of the first-stage extraction column is connected to a first storage module via a first three-way switching valve, and the output end of the second-stage extraction column is connected to a second storage module via a second three-way switching valve.

[0006] Preferably, the sample supply module includes a first acid reagent, a first cleaning reagent, a second cleaning reagent, and a seaweed aquaculture water sample, and the output end of the reagent supply module is connected to the filter column via an injection pump.

[0007] Preferably, the first-stage elution module includes a first purge gas, a second acid reagent, a base reagent, and a first organic reagent. The output end of the first purge gas is connected to the input end of the first-stage extraction column via a first purge gas pump. The output end of the second acid reagent is connected to the input end of the first-stage extraction column via a second acid reagent pump. The output end of the base reagent is connected to the input end of the first-stage extraction column via a base reagent pump. The output end of the first organic reagent is connected to the input end of the first-stage extraction column via a first organic reagent pump.

[0008] Preferably, the second-stage elution module includes a second purge gas and a second organic reagent. The output end of the second purge gas is connected to the input end of the second-stage extraction column via a second purge gas pump, and the output end of the second organic reagent is connected to the input end of the second-stage extraction column via a second organic reagent pump.

[0009] Preferably, the filter media of the filter column is a glass fiber membrane with a pore size of 0.45 micrometers, the packing material of the first-stage extraction column is macroporous adsorption resin DAX-8, and the packing material of the second-stage extraction column is HLB hydrophilic-lipophilic balanced packing material.

[0010] Preferably, in the sample supply module, the first acid reagent is a 0.01 mol / L hydrochloric acid solution, the first cleaning reagent is distilled water, and the second cleaning reagent is methanol.

[0011] Preferably, in the first-stage elution module, the first purge gas is nitrogen, the second acid reagent is 0.1 mol / L hydrochloric acid solution, the base reagent is 0.1 mol / L NaOH solution, and the first organic reagent is methanol.

[0012] Preferably, in the second-stage elution module, the second purge gas is nitrogen and the second organic reagent is methanol.

[0013] A method for operating an apparatus for extracting and separating organic carbon components from kelp aquaculture water includes the following steps: S1. Prepare 0.01 mol / L hydrochloric acid solution, distilled water and methanol, and inject them into the corresponding reagent bottles of the first acid reagent, cleaning reagent one and cleaning reagent two. Extract kelp farming water samples and inject them into the corresponding reagent bottles of kelp farming water samples. S2. Start the injection pump and sequentially clean the first-stage extraction column of the activated macroporous adsorption resin DAX-8 and the second-stage extraction column of the hydrophilic-lipophilic balanced packing HLB with the first acid reagent, cleaning reagent one, cleaning reagent two and cleaning reagent one. S3. Start the reagent supply module and deliver 30 mL of kelp aquaculture water sample through a syringe pump at a flow rate of 2.0 mL / min. The sample will pass through a filter column, a first-stage extraction column, and a second-stage extraction column in sequence to collect POC and DOC from the kelp aquaculture water sample. Then, pump 10 mL of 0.01 mol / L hydrochloric acid solution into the filter column, the first-stage extraction column, and the second-stage extraction column at a flow rate of 1.0 mL / min to remove inorganic carbonates and prevent CO2 from dissolving in the air. The effluent will be collected in the waste liquid collection module. S4. Start the first-stage elution module, turn on the first purge gas, and use nitrogen to dry the first-stage extraction column until the adsorbent turns white inside the column. In the second-dimensional mode, use the first purge gas pump, the second acid reagent pump, the alkali reagent pump, and the first organic reagent pump to continuously rinse the first-stage extraction column with 3 mL of 0.1 mol / L HCl solution, 3 mL of 0.1 mol / L NaOH solution, and 5 mL of methanol at a flow rate of 0.3 mL / min. Before each change of elution reagent, turn on the first purge gas to dry the first-stage extraction column with nitrogen. The eluent obtained at the output end of the first-stage extraction column is collected in the first storage module through the first three-way switching valve to achieve the elution and separation of hydrophobic organic carbon HPO. S5. Start the second-stage elution module, turn on the second purge gas nitrogen to dry the second-stage extraction column until the adsorbent turns white in the column. In the second-dimensional mode, use the second purge gas pump and the second organic reagent pump to rinse the second-stage extraction column with 5 mL of the second organic reagent methanol at a flow rate of 0.3 mL / min. Turn on the first purge gas nitrogen to dry the second-stage extraction column. The eluent obtained at the output end of the second-stage extraction column is collected in the second storage module through the second three-way switching valve to achieve the elution and separation of hydrophilic and transitional hydrophilic organic carbon substances HPI and TPI. S6. After completing the test, replace filter column 2, primary extraction column and secondary extraction column, and repeat steps 3-6 to carry out a new round of extraction and separation of organic carbon components in kelp aquaculture water samples.

[0014] Compared with existing technologies, this invention has the following advantages: Based on a first-dimensional hierarchical extraction system and a second-dimensional elution separation system, the first-dimensional system enables continuous capture of particulate carbon (POC), removal of inorganic carbonates (IC), and extraction of dissolved organic carbon (DOC) in kelp aquaculture water on a filtration column and two-stage extraction columns. Simultaneously, the second-dimensional system enables purge and elution of DOCs of different polarities, achieving hierarchical extraction and separation of hydrophilic and hydrophobic DOCs in kelp aquaculture water. Compared with existing DOC extraction technologies reported in the literature, which mainly use hydrophilic and lipophilic polymer stationary phases as separation columns for total DOC analysis of all types, this invention can achieve hierarchical separation of DOCs of different polarities. This facilitates the analysis of different types of organic carbon, such as humic substances and protein-like substances, in kelp aquaculture water and the tracing of carbon sources, making DOC extraction and analysis in water more efficient.

[0015] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0016] Figure 1 This is a schematic diagram illustrating the structure of an embodiment of the present invention. Detailed Implementation

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0018] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0019] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0020] like Figure 1As shown, this embodiment provides an apparatus for extracting and separating organic carbon components in kelp aquaculture water, including a first-dimensional graded extraction system and a second-dimensional elution separation system. The first-dimensional graded extraction system includes a sample supply module 1, a filter column 2, a first-stage extraction column 3, and a second-stage extraction column 4. The output end of the sample supply module 1 is sequentially connected to the filter column 2, the first-stage extraction column 3, the second-stage extraction column 4, and a waste liquid collection module 5 along the liquid flow direction. The second-dimensional elution separation system includes a first-stage elution module 6 and a second-stage elution module 7. The output end of the first-stage elution module 6 is connected between the filter column 2 and the first-stage extraction column 3, and the output end of the second-stage elution module 7 is connected between the first-stage extraction column 3 and the second-stage extraction column 4. The output end of the first-stage extraction column 3 is connected to the first storage module 9 via a first three-way switching valve 11, and the output end of the second-stage extraction column 4 is connected to the second storage module 10 via a second three-way switching valve 12.

[0021] The kelp aquaculture water sample output by the sample supply module 1 is sequentially subjected to kelp particulate carbon (POC) capture, inorganic carbonate (IC) removal, and dissolved organic carbon (DOC) extraction in a graded extraction system consisting of a filter column 2, a first-stage extraction column 3, and a second-stage extraction column 4. The first-stage elution module 6 and the second-stage elution module 7 sequentially purge and elute dissolved organic carbon substances of different polarities in the first-stage extraction column 3 and the second-stage extraction column 4, respectively, to achieve continuous graded extraction and separation of hydrophilic and hydrophobic DOC in the kelp aquaculture water.

[0022] In this embodiment of the invention, the sample supply module 1 includes a first acid reagent 101, a first cleaning reagent 102, a second cleaning reagent 103, and a seaweed aquaculture water sample 104. The output end of the reagent supply module 1 is connected to the filter column 2 via an injection pump 801.

[0023] In the first-dimensional mode, the filtration and separation of particulate carbon and dissolved carbon components in kelp aquaculture water, the removal of inorganic carbonate carbon, and the fractional extraction of hydrophilic and hydrophobic DOC are achieved.

[0024] In this embodiment of the invention, the first-stage elution module 6 includes a first purge gas 601, a second acid reagent 602, an alkaline reagent 603, and a first organic reagent 604. The output end of the first purge gas 601 is connected to the input end of the first-stage extraction column 3 via a first purge gas pump 802. The output end of the second acid reagent 602 is connected to the input end of the first-stage extraction column 3 via a second acid reagent pump 803. The output end of the alkaline reagent 603 is connected to the input end of the first-stage extraction column 3 via an alkaline reagent pump 804. The output end of the first organic reagent 604 is connected to the input end of the first-stage extraction column 3 via a first organic reagent pump 805.

[0025] In the second dimension mode, the residual solution in the first-stage extraction column 3 after extraction is purged and removed, and the hydrophobic organic carbon substance HPO on the first-stage extraction column 3 is eluted. The eluent is stored in the first storage module 9.

[0026] In this embodiment of the invention, the second-stage elution module 7 includes a second purge gas 701 and a second organic reagent 702. The output end of the second purge gas 701 is connected to the input end of the second-stage extraction column 4 via a second purge gas pump 806, and the output end of the second organic reagent 702 is connected to the input end of the second-stage extraction column 4 via a second organic reagent pump 807.

[0027] In the second dimension mode, the residual solution in the second-stage extraction column 4 after extraction is purged and removed, and the hydrophilic organic carbon HPI and transitional hydrophilic organic carbon TPI on the second-stage extraction column 4 are eluted. The eluent is stored in the second storage module 10.

[0028] In this embodiment of the invention, the filter media of the filter column 2 is a glass fiber membrane with a pore size of 0.45 micrometers, the packing material of the first-stage extraction column 3 is macroporous adsorption resin DAX-8, and the packing material of the second-stage extraction column 4 is HLB hydrophilic-lipophilic balanced packing material.

[0029] In this embodiment of the invention, the first acid reagent 101 in the sample supply module 1 is a 0.01 mol / L hydrochloric acid solution, the first cleaning reagent 102 is distilled water, and the second cleaning reagent 103 is methanol.

[0030] In this embodiment of the invention, the first purge gas 601 in the first-stage elution module 6 is nitrogen, the second acid reagent 602 is a 0.1 mol / L hydrochloric acid solution, the base reagent 603 is a 0.1 mol / L NaOH solution, and the first organic reagent 604 is methanol.

[0031] In this embodiment of the invention, the second purge gas 701 in the second-stage elution module 7 is nitrogen, and the second organic reagent 702 is methanol.

[0032] A method for operating an apparatus for extracting and separating organic carbon components from kelp aquaculture water comprises the following steps: S1. Prepare 0.01 mol / L hydrochloric acid solution, distilled water and methanol, and inject them into the corresponding reagent bottles of the first acid reagent, cleaning reagent one and cleaning reagent two. Extract kelp farming water samples and inject them into the corresponding reagent bottles of kelp farming water samples. S2. Start the injection pump 801 and sequentially clean the first-stage extraction column 3 of the activated macroporous adsorption resin DAX-8 and the second-stage extraction column 4 of the hydrophilic-lipophilic balanced packing HLB with the first acid reagent 101, cleaning reagent 102, cleaning reagent 2 103 and cleaning reagent 102. S3. Start the reagent supply module and deliver 30 mL of kelp aquaculture water sample through syringe pump 801 at a flow rate of 2.0 mL / min. The sample will pass through filter column 2, first-stage extraction column 3, and second-stage extraction column 4 in sequence to collect POC and DOC from the kelp aquaculture water sample. Then, pump 10 mL of 0.01 mol / L hydrochloric acid solution into filter column 2, first-stage extraction column 3, and second-stage extraction column 4 at a flow rate of 1.0 mL / min to remove inorganic carbonates and prevent CO2 from dissolving in the air. The effluent will be collected in waste liquid collection module 5. S4. Start the first-stage elution module 6, turn on the first purge gas 601, and use nitrogen to dry the first-stage extraction column 3 until the adsorbent turns white inside the column. In the second-dimensional mode, the first-stage extraction column 3 is continuously eluted with 3 mL of 0.1 mol / L HCl solution, 3 mL of 0.1 mol / L NaOH solution, and 5 mL of methanol at a flow rate of 0.3 mL / min by the first purge gas pump 802, the second acid reagent pump 803, the alkali reagent pump 804, and the first organic reagent pump 805. Before each change of elution reagent, turn on the first purge gas 601 to dry the first-stage extraction column 3 with nitrogen. The eluent obtained at the output end of the first-stage extraction column 3 is collected in the first storage module 9 through the first three-way switching valve 11 to achieve the elution and separation of hydrophobic organic carbon HPO. S5. Start the second-stage elution module 7, turn on the nitrogen of the second purge gas 701 to dry the second-stage extraction column 4 until the adsorbent turns white in the column. In the second dimension mode, the second-stage extraction column 4 is rinsed with 5 mL of methanol of the second organic reagent 702 at a flow rate of 0.3 mL / min through the second purge gas pump 806 and the second organic reagent pump 807. The second-stage extraction column 4 is dried with nitrogen of the first purge gas 601. The eluent obtained at the output end of the second-stage extraction column 4 is collected in the second storage module 10 through the second three-way switching valve 12 to achieve the elution and separation of hydrophilic and transitional hydrophilic organic carbon substances HPI and TPI. S6. After completing the test, replace filter column 2, primary extraction column 3, and secondary extraction column 4, and repeat steps 3-6 to carry out a new round of extraction and separation of organic carbon components in kelp aquaculture water samples.

[0033] In this embodiment of the invention, in step 2, the first acid reagent is a 0.01 mol / L hydrochloric acid solution, the first cleaning reagent is distilled water, and the second cleaning reagent is methanol.

[0034] In this embodiment of the invention, in step 4, the second acid reagent is a 0.1 mol / L HCl solution, the base reagent is a 0.1 mol / L NaOH solution, and the first organic reagent is methanol.

[0035] In this embodiment of the invention, the purging gas in step 5 is nitrogen, and the second organic reagent is methanol.

[0036] Specific implementation process: S1. Open the sample supply module 1 and use the syringe pump 801 to sequentially feed 10 mL of 0.01 mol / L hydrochloric acid solution, 10 mL of distilled water, 10 mL of methanol, and 10 mL of distilled water into the glass fiber membrane of the filter column 2 (0.45 μm pore size), the first-stage extraction column 3 (3 mL, Φ10 mm × 100 mm) of macroporous adsorption resin DAX-8, and the second-stage extraction column 4 (3 mL, Φ10 mm × 100 mm) of hydrophilic-lipophilic balanced packing HLB. Clean and activate the filter column and the two-stage extraction columns. S2. Collect 30 mL of kelp aquaculture water sample at a flow rate of 2.0 mL / min, and pass it sequentially through filter column 2, first-stage extraction column 3, and second-stage extraction column 4. Then, pump 10 mL of 0.01 mol / L hydrochloric acid solution into filter column 2, first-stage extraction column 3, and second-stage extraction column 4 at a flow rate of 1.0 mL / min to achieve POC collection, DOC capture, and inorganic carbonate removal from the kelp aquaculture water sample. S3. Start the first-stage elution module 6, turn on nitrogen to dry the first-stage extraction column 3 until the adsorbent turns white inside the column, and continuously rinse the first-stage extraction column 3 with 3 mL of 0.1 mol / L HCl solution, 3 mL of 0.1 mol / L NaOH solution and 5 mL of methanol at a flow rate of 0.3 mL / min. Before each change of elution reagent, dry the first-stage extraction column 3 with nitrogen. The resulting eluent is collected in the first storage module 9 through the first three-way switching valve 11 to achieve the extraction and separation of hydrophobic organic carbon HPO. S4. Selectively and synchronously start the second-stage elution module 7, turn on nitrogen to dry the second-stage extraction column 4 until the adsorbent turns white in the column, rinse the second-stage extraction column 4 with 5 mL of methanol at a flow rate of 0.3 mL / min, turn on nitrogen to dry the second-stage extraction column 4, and collect the eluent in the second storage module 10 through the second three-way switching valve 12 to achieve the extraction and separation of hydrophilic and transitional hydrophilic organic carbon substances HPI and TPI. S5. After completing the test, replace filter column 2, first-stage extraction column 3, and second-stage extraction column 4, and repeat the above steps to extract and separate different polar organic carbon components from the kelp aquaculture water sample for a new round.

[0037] This invention is based on a first-dimensional fractional extraction system and a second-dimensional elution separation system. The first-dimensional fractional extraction system continuously captures particulate carbon (POC), removes inorganic carbonates (IC), and extracts dissolved organic carbon (DOC) from aquaculture seawater via its filtration column and two-stage extraction columns. The second-dimensional elution system then elutes the DOC from the two extraction columns, achieving fractional extraction and separation of hydrophilic and hydrophobic DOC in kelp aquaculture water. This invention can continuously achieve fractional separation of DOCs of different polarities. Compared with existing technologies that cannot distinguish between different polarities of DOC and only perform total analysis of all types of DOC, this invention is more efficient for the extraction and separation of DOC components in seawater, facilitating the analysis and traceability of different types of organic carbon, such as humic substances and protein-like substances, in kelp aquaculture water.

[0038] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A method for operating a device for extracting and separating organic carbon components in kelp aquaculture water, characterized in that: The system includes a first-dimensional fractional extraction system and a second-dimensional elution separation system. The first-dimensional fractional extraction system includes a sample supply module (1), a filter column (2), a first-stage extraction column (3), and a second-stage extraction column (4). The output end of the sample supply module (1) is sequentially connected to the filter column (2), the first-stage extraction column (3), the second-stage extraction column (4), and a waste liquid collection module (5) along the liquid flow direction. The second-dimensional elution separation system includes a first-stage elution module (6) and a second-stage elution module (7). The output end of the first-stage elution module (6) is connected between the filter column (2) and the first-stage extraction column (3). The output end of the second-stage elution module (7) is connected between the first-stage extraction column (3) and the second-stage extraction column (4). The output end of the first-stage extraction column (3) is connected to the first storage module via a first three-way switching valve (11). (9) Connection: The output end of the second-stage extraction column (4) is connected to the second storage module (10) via the second three-way switching valve (12); The sample supply module (1) includes a first acid reagent (101), a first cleaning reagent (102), a second cleaning reagent (103), and a seaweed aquaculture water sample (104). The output end of the reagent supply module (1) is connected to the filter column (2) via an injection pump (801); The second-stage elution module (7) includes a second purge gas (701) and a second organic reagent (702). The output end of the second purge gas (701) is connected to the input end of the second-stage extraction column (4) via a second purge gas pump (806), and the output end of the second organic reagent (702) is connected to the input end of the second-stage extraction column (4) via a second organic reagent pump (807); The working method is carried out according to the following steps: S1. Prepare 0.01 mol / L hydrochloric acid solution, distilled water and methanol, and inject them into the corresponding reagent bottles of the first acid reagent, cleaning reagent one and cleaning reagent two. Extract kelp farming water samples and inject them into the corresponding reagent bottles of kelp farming water samples. S2. Start the injection pump (801) and sequentially clean the first-stage extraction column (3) of the activated macroporous adsorption resin DAX-8 and the second-stage extraction column (4) of the hydrophilic and lipophilic balanced packing HLB with the first acid reagent (101), cleaning reagent one (102), cleaning reagent two (103) and cleaning reagent one (102). S3. Start the reagent supply module and deliver 30 mL of kelp aquaculture water sample (104) through the syringe pump (801) at a flow rate of 2.0 mL / min. The sample passes through the filter column (2), the first-stage extraction column (3), and the second-stage extraction column (4) in sequence to collect POC and DOC in the kelp aquaculture water sample. Then, pump 10 mL of 0.01 mol / L hydrochloric acid solution into the filter column (2), the first-stage extraction column (3), and the second-stage extraction column (4) at a flow rate of 1.0 mL / min to remove inorganic carbonates and prevent CO2 from dissolving in the air. The effluent is collected in the waste liquid collection module (5). S4. Start the first-stage elution module (6), turn on the first purge gas (601), and blow the first-stage extraction column (3) with nitrogen until the adsorbent turns white in the column. In the second dimension mode, the first-stage extraction column (3) is continuously eluted with 3 mL of 0.1 mol / L HCl solution of the second acid reagent (602), 3 mL of 0.1 mol / L NaOH solution of the alkali reagent (603), and 5 mL of methanol of the first organic reagent (604) at a flow rate of 0.3 mL / min by the first purge gas pump (802), the second acid reagent pump (803), the alkali reagent pump (804), and the first organic reagent pump (805). Before each change of elution reagent, turn on the first purge gas (601) to blow the first-stage extraction column (3) with nitrogen. The eluent obtained at the output end of the first-stage extraction column (3) is collected in the first storage module (9) through the first three-way switching valve (11) to realize the elution and separation of hydrophobic organic carbon HPO. S5. Start the second-stage elution module (7), turn on the second purge gas (701) to blow the nitrogen gas of the second stage extraction column (4) until the adsorbent turns white in the column. In the second dimension mode, the second purge gas pump (806) and the second organic reagent pump (807) are used to rinse the second-stage extraction column (4) with 5 mL of methanol of the second organic reagent (702) at a flow rate of 0.3 mL / min. Turn on the first purge gas (601) to blow the nitrogen gas of the second stage extraction column (4) dry. The eluent obtained at the output end of the second stage extraction column (4) is collected in the second storage module (10) through the second three-way switching valve (12) to realize the elution and separation of hydrophilic and transitional hydrophilic organic carbon substances HPI and TPI. S6. After completing the test, replace filter column 2, primary extraction column (3) and secondary extraction column (4), and repeat steps 3-6 to carry out a new round of extraction and separation of organic carbon components in kelp aquaculture water samples.

2. The working method of the device for extracting and separating organic carbon components in kelp aquaculture water according to claim 1, characterized in that: The first-stage elution module (6) includes a first purge gas (601), a second acid reagent (602), an alkaline reagent (603), and a first organic reagent (604). The output of the first purge gas (601) is connected to the input of the first-stage extraction column (3) via a first purge gas pump (802). The output of the second acid reagent (602) is connected to the input of the first-stage extraction column (3) via a second acid reagent pump (803). The output of the alkaline reagent (603) is connected to the input of the first-stage extraction column (3) via an alkaline reagent pump (804). The output of the first organic reagent (604) is connected to the input of the first-stage extraction column (3) via a first organic reagent pump (805).

3. The working method of the device for extracting and separating organic carbon components in kelp aquaculture water according to claim 1, characterized in that: The filter media of the filter column (2) is a glass fiber membrane with a pore size of 0.45 micrometers, the packing material of the first-stage extraction column (3) is macroporous adsorption resin DAX-8, and the packing material of the second-stage extraction column (4) is HLB hydrophilic and lipophilic balanced packing material.

4. The working method of the device for extracting and separating organic carbon components in kelp aquaculture water according to claim 1, characterized in that: In the sample supply module (1), the first acid reagent (101) is a 0.01 mol / L hydrochloric acid solution, the first cleaning reagent (102) is distilled water, and the second cleaning reagent (103) is methanol.

5. The working method of the device for extracting and separating organic carbon components in kelp aquaculture water according to claim 2, characterized in that: In the first-stage elution module (6), the first purge gas (601) is nitrogen, the second acid reagent (602) is 0.1 mol / L hydrochloric acid solution, the base reagent (603) is 0.1 mol / L NaOH solution, and the first organic reagent (604) is methanol.

6. The working method of the device for extracting and separating organic carbon components in kelp aquaculture water according to claim 1, characterized in that: In the second stage elution module (7), the second purge gas (701) is nitrogen, and the second organic reagent (702) is methanol.

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