A tuberculosis detection device and method of use thereof

Abstract

This invention discloses a tuberculosis detection device, which includes a body with a mounting plate inserted into it. A laser detector is mounted on the top of the mounting plate, and chromatography paper is installed inside the mounting plate. A winding roller is rotatably connected to one end of the mounting plate, and a flow guide block is inserted into the other end of the mounting plate. An elevated receiving box is installed inside the flow guide block, and a piston is slidably connected inside the receiving box. When detecting plasma, the mounting plate, flow guide block, insertion block, and connecting block are inserted together. Filtration can be selected as needed, allowing for the selection of a suitable insertion block, providing high flexibility. After the baffle closes, blood spillage is effectively prevented, improving detection safety. The receiving box is heated by a water bath, resulting in more stable temperature, facilitating culture or PCR amplification. The chromatography paper can be folded and released, ensuring the continuity and accuracy of chromatography.

Description

Technical Field

[0001] This invention relates to the field of tuberculosis detection technology, and in particular to a tuberculosis detection device and its usage method. Background Technology

[0002] Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. While it was effectively controlled for a period, its incidence has been increasing in recent years, making it a serious infectious disease threatening human health and life once again, drawing widespread attention worldwide. Therefore, TB testing is of paramount importance. Interferon-gamma release assay (IGRA) is a novel in vitro immunoassay method for detecting Mycobacterium tuberculosis infection. It examines the presence of specific effector T lymphocytes in TB-infected individuals. When these effector T lymphocytes are stimulated again by TB antigens, they secrete the cytokine interferon-gamma (INF). This method is simple, rapid, sensitive, and accurate in detecting intrapulmonary and extrapulmonary TB.

[0003] A search revealed a Chinese invention patent, publication number CN113533738B, entitled "Tuberculosis Detection Device and Detection Method." This invention comprises: a first microfluidic chip layer including a reservoir module with two independent cavities for containing whole blood and buffer solution, respectively; a second microfluidic chip layer including a mixing reaction module with two inlets, the mixing reaction module containing a mixing channel and a chromatographic test piece; the first end of the mixing channel is connected to both inlets, and the second end is connected to the chromatographic test piece, configured to mix plasma and buffer solution to form a mixed reagent, and guide the mixed reagent to the chromatographic test piece for a chromatographic reaction; at least two fluid drive components configured to supply plasma and buffer solution from the reservoir module to the corresponding mixing reaction module through the two inlets; and a fluorescence detection component configured to detect the reagent after the chromatographic reaction.

[0004] However, in actual use, traditional and similar technical solutions still have some problems: 1. For this type of detection technology, in the process of collecting, transporting and processing blood samples, the relevant equipment will inevitably come into direct contact with the blood samples. This contact characteristic requires that the equipment must be thoroughly cleaned after each test of a single sample in order to avoid cross-contamination between samples. However, this cleaning step significantly increases the time cost of the overall detection process, which in turn has an adverse impact on the detection efficiency. 2. In practical testing, the length of chromatography paper, as a key testing medium, is preset and fixed. When an abnormal signal is observed during the testing process and further high-precision analysis is required, the existing length of chromatography paper may not meet the more detailed testing needs. At this time, the operator needs to replace it with a longer chromatography paper that can provide a larger testing area and repeat the entire testing process. This step is not only cumbersome, but also greatly reduces the flexibility and efficiency of the testing work. 3. When using electric heating for sample processing or testing, the accuracy of temperature control is extremely critical. Temperature fluctuations or instability in the electric heating system may lead to inconsistencies in sample processing, thereby affecting the accuracy and reliability of the test results. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology by proposing a tuberculosis detection device and its usage method that features precise temperature control, no blood-device contact, and adjustable chromatography paper length.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A tuberculosis detection device includes a body with a laser detection mechanism connected to it. The laser detection mechanism includes a mounting plate, which is inserted into the body. A laser detector is mounted on the top of the mounting plate. Chromatography paper is disposed inside the mounting plate. A take-up roller is rotatably connected to one end of the mounting plate. A spiral spring is installed between the take-up roller and the mounting plate. The chromatography paper is wound onto the take-up roller, and the free end of the chromatography paper is fixed to the mounting plate. A pull roller is slidably connected to the mounting plate, and the chromatography paper is wound around the pull roller. A drive assembly is connected to the mounting plate. A flow guiding mechanism is connected to the mounting plate. The flow guiding mechanism includes a flow guiding block. One end of the mounting plate is inserted into the flow guiding block. An elevated receiving box is installed inside the flow guiding block. Both ends of the receiving box are connected to connecting pipes. A one-way valve is installed on the connecting pipe. The receiving box is unidirectionally connected to the chromatography paper end of the mounting plate through the connecting pipe and the one-way valve. A piston is slidably connected inside the receiving box. A pushing component is connected to the piston. A water pipe is inserted into the flow guiding block. An addition pipe is inserted into the receiving box. A one-way valve is also installed at the end of the receiving box where the addition pipe is inserted.

[0007] Preferably, the pulling assembly includes a lead screw, which is rotatably connected to the mounting plate. A slider is threaded onto the lead screw, one end of the pulling roller is fixedly connected to the slider, and a motor is mounted on the machine body. One end of the lead screw is inserted into the output shaft of the motor.

[0008] Preferably, a guide roller is mounted on the mounting plate near the free end of the chromatography paper, and the chromatography paper is wound and connected to the guide roller.

[0009] Preferably, the pushing assembly includes a third spring, a third spring is installed between the piston and the receiving box, a fifth electric push rod is installed on the body, and the telescopic end of the fifth electric push rod abuts against the piston.

[0010] Preferably, the machine body is connected to a temperature control mechanism, the temperature control mechanism includes a temperature-controlled heating water tank, a pair of water pipes are provided, the other end of the pair of water pipes is installed on the temperature-controlled heating water tank, a second water pump is installed on one of the water pipes, a fourth electric push rod is installed on the machine body, and the plug ends of the pair of water pipes are fixed on the telescopic end of the fourth electric push rod.

[0011] Preferably, the machine body is connected to an adding mechanism, the adding mechanism includes a storage box, one end of the machine body is installed with the storage box, the adding tube is installed on the storage box, a first water pump is installed on the adding tube, a third electric push rod is installed on the machine body, and the plug end of the adding tube is installed on the telescopic end of the third electric push rod.

[0012] Preferably, the end of the guide block opposite to the mounting plate is equipped with a connecting block, the connecting block is equipped with a suction tube, the guide block is connected to a filter mechanism, the filter mechanism includes a plug-in block, a plug-in block is inserted between the guide block and the connecting block, and the connecting tube is connected to the suction tube through the plug-in block.

[0013] Preferably, the plug block is equipped with a blocking filter membrane, which is a 0.45 µm PES membrane.

[0014] Preferably, the connecting block is rotatably connected to a sampling mechanism, the sampling mechanism including a sampling block, the connecting block being rotatably connected to the sampling block, an insertion tube being slidably connected inside the sampling block, a second spring being installed between the insertion tube and the sampling block, one end of the suction tube being installed on the insertion tube, a first electric push rod being installed on the machine body, the telescopic end of the first electric push rod abutting against one end of the insertion tube, a blocking baffle being slidably connected to the bottom end of the sampling block, a first spring being installed between the blocking baffle and the sampling block, a through hole being provided on the blocking baffle, the insertion tube communicating with the outside through the through hole, a second electric push rod being installed on the machine body, the telescopic end of the second electric push rod abutting against the blocking baffle, and the machine body clamping the sampling tube below the insertion tube.

[0015] The tuberculosis detection device and its method of use include the following steps: S1. When testing plasma, insert the mounting plate, guide block, insertion block and connecting block together, bend the sampling block downwards to make the sampling block fit against the body, start the second electric push rod to push the blocking baffle to slide, the through hole moves to the bottom of the tube, start the first electric push rod to push the tube to slide downwards, and the bottom of the tube extends into the sampling tube. S2. Before drawing blood, activate the fifth electric push rod, the piston moves to expel the air inside the container, the fifth electric push rod retracts, the piston returns to its original position, and the blood in the sampling tube is drawn into the container. Activate the first water pump, and the buffer solution inside the container is pressed into the container through the addition tube. The buffer solution mixes with the plasma. Activate the second water pump, and the warm water inside the temperature-controlled heating tank enters the guide block through the water pipe to heat the container in a water bath. S3. Activate the fifth electric push rod. The piston pushes the plasma into the interior of the mounting plate. The plasma contacts the fixed end of the chromatography paper and begins chromatography. The analysis is checked by a laser detector. Start the motor. The motor rotates, driving the lead screw to rotate. The slider drives the pull roller to move. The pull roller pulls the chromatography paper to fold. The take-up roller releases, the spiral spring is compressed, and a new end of the chromatography paper is exposed. The chromatography continues.

[0016] Compared with the prior art, the present invention provides a tuberculosis detection device, which has the following beneficial effects: 1. When testing plasma, the mounting plate, guide block, plug-in block, and connecting block are plugged together. Filtering can be selected as needed, allowing for the selection of a suitable plug-in block. Without filtration, the selected plug-in block has no internal filter membrane, offering high flexibility. Using the plug-in method, blood does not come into contact with the machine during the entire laser detection process, effectively ensuring the accuracy of the next set of test data. Furthermore, no machine cleaning is required, resulting in high safety. Laser detection eliminates the need to add antibody proteins to the chromatography paper, expanding the detection range. Insert the entire assembly into the machine body, bend the sampling block downwards to clamp the sampling tube containing whole blood onto the machine body, activate the second electric push rod to open the blocking baffle, and activate the first electric push rod to insert the bottom end of the tube into the sampling tube, facilitating blood extraction. After the blocking baffle closes, blood spillage is effectively prevented, improving detection safety.

[0017] 2. Before drawing blood, activate the fifth electric push rod. The piston moves, expelling all air from the container. The fifth electric push rod retracts, and the piston returns to its original position, creating negative pressure inside the container. This draws the blood from the sampling tube into the insertion tube. The blood then enters the insertion block and is filtered through a filter membrane to extract plasma. The plasma enters the container. Activate the first water pump, which collects the buffer solution from inside the container and feeds it into the addition tube. This buffer solution is then forced into the container through a one-way valve, mixing with the plasma for easier culture. Activate the temperature-controlled heating water tank to heat the water to a suitable temperature. Activate the second water pump, which then feeds warm water through a pipe into the guide block, facilitating water bath heating of the container for more stable temperature control, thus aiding in culture or PCR amplification.

[0018] 3. Activate the fifth electric push rod, which pushes the piston. Plasma is forced into the mounting plate through the one-way valve. The plasma contacts the fixed end of the chromatography paper, facilitating chromatography. The analysis can be performed using a laser detector. When increased chromatography accuracy is required, and the existing length of the chromatography paper is insufficient for the test conditions, the exposed end of the chromatography paper will be used up. At this time, the lead screw is connected to the output shaft of the motor, and the motor is started. The slider drives the pull roller to move. Under the action of the guide roller, the pull roller pulls the chromatography paper to fold. During plasma chromatography, as the folded end of the chromatography paper is passed, the chromatographic area becomes longer, and the number of folded ends increases. The take-up roller releases, and a new end of the chromatography paper is exposed, thus ensuring the continuity and accuracy of the chromatography. Attached Figure Description

[0019] Figure 1 This is a perspective view of a tuberculosis detection device and its usage method proposed in this invention; Figure 2 This is a view of the connection structure of the body of the present invention; Figure 3 This is a view of the connection structure of the sampling block of the present invention; Figure 4 This is a view of the connection structure of the laser detector of the present invention; Figure 5 This is a view of the connection structure of the flow guiding mechanism of the present invention; Figure 6 This is a view of the connection structure of the cannula of the present invention; Figure 7 This is a view of the connection structure of the flow guide block of the present invention; Figure 8 This is a view of the connection structure of the mounting plate of the present invention; Figure 9 This is a view of the piston connection structure of the present invention; Figure 10 This is a view of the connection structure of the chromatography paper of the present invention.

[0020] In the diagram: 1. Main body; 2. Sampling tube; 3. Sampling mechanism; 31. Sampling block; 32. First electric push rod; 33. Second electric push rod; 34. Insertion tube; 35. Blocking baffle; 36. First spring; 37. Through hole; 38. Suction tube; 39. Second spring; 4. Addition mechanism; 41. Storage box; 42. First water pump; 43. Addition tube; 44. Third electric push rod; 5. Temperature control mechanism; 51. Temperature-controlled heating water tank; 52. Water pipe; 53. Second water pump 54. Fourth electric push rod; 6. Laser detection mechanism; 61. Laser detector; 62. Mounting plate; 63. Motor; 64. Chromatography paper; 65. Scroll spring; 66. Lead screw; 67. Take-up roller; 68. Guide roller; 69. Pull roller; 7. Connecting block; 8. Filtering mechanism; 81. Insertion block; 82. Filter membrane; 9. Flow guiding mechanism; 91. Fifth electric push rod; 92. Flow guiding block; 93. Receiving box; 94. Piston; 95. Third spring; 96. Connecting pipe. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0022] In the description of this invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0023] Example 1: Refer to Figures 1-10 A tuberculosis detection device includes a body 1, a laser detection mechanism 6 connected to the body 1, and a mounting plate 62. The mounting plate 62 is inserted into the body 1, and a laser detector 61, which is a Raman spectroscopy laser detector, is mounted on the top of the mounting plate 62 and connected to an external Raman spectrometer. Chromatography paper 64 is provided inside the mounting plate 62, and a take-up roller 67 is rotatably connected to one end of the mounting plate 62. A spiral spring 65 is installed between the take-up roller 67 and the mounting plate 62. The chromatography paper 64 is wound onto the take-up roller 67, and the free end of the chromatography paper 64 is fixed to the mounting plate 62. A pull roller 69 is slidably connected to the mounting plate 62, and the chromatography paper 64 is wound around the pull roller 69. A drive assembly is connected to the mounting plate 62, thereby facilitating the detection of plasma through the chromatography paper 64.

[0024] In this invention, the pulling assembly includes a lead screw 66, which is rotatably connected to a mounting plate 62. A slider is threaded onto the lead screw 66, and one end of a pulling roller 69 is fixedly connected to the slider. A motor 63 is mounted on the machine body 1, and one end of the lead screw 66 is inserted into the output shaft of the motor 63. Driving the lead screw 66 to rotate will drive the pulling roller 69 to move, thereby folding the chromatography paper 64 and increasing the exposed length of the chromatography paper 64, thus facilitating high-precision chromatography detection.

[0025] In this invention, a guide roller 68 is installed on the mounting plate 62 near the free end of the chromatography paper 64. The chromatography paper 64 is wound and connected with the guide roller 68, thereby increasing the smoothness of folding of the chromatography paper 64 and avoiding mutual contact.

[0026] Example 2: Based on Example 1, a tuberculosis detection device is provided. A flow guiding mechanism 9 is connected to a mounting plate 62. The flow guiding mechanism 9 includes a flow guiding block 92. The flow guiding block 92 is inserted into one end of the mounting plate 62. An elevated receiving box 93 is installed inside the flow guiding block 92. Both ends of the receiving box 93 are equipped with connecting pipes 96. A one-way valve is installed on the connecting pipes 96. The receiving box 93 is unidirectionally connected to the chromatography paper 64 end of the mounting plate 62 through the connecting pipes 96 and the one-way valve. A piston 94 is slidably connected inside the receiving box 93. A pushing component is connected to the piston 94. A water pipe 52 is inserted into the flow guiding block 92. An adding pipe 43 is inserted into the receiving box 93. A one-way valve is also installed at the end of the receiving box 93 where it is inserted into the adding pipe 43, thereby facilitating the control of whole blood extraction and plasma propulsion through the movement of the piston 94.

[0027] In this invention, the pushing component includes a third spring 95, and the third spring 95 is installed between the piston 94 and the receiving box 93. A fifth electric push rod 91 is installed on the body 1, and the telescopic end of the fifth electric push rod 91 abuts against the piston 94, thereby facilitating the automatic reset of the piston 94.

[0028] In this invention, a temperature control mechanism 5 is connected to the body 1. The temperature control mechanism 5 includes a temperature-controlled heating water tank 51 and a pair of water pipes 52. The other end of the pair of water pipes 52 is installed on the temperature-controlled heating water tank 51. A second water pump 53 is installed on one of the water pipes 52. A fourth electric push rod 54 is installed on the body 1. The insertion ends of the pair of water pipes 52 are fixed to the telescopic end of the fourth electric push rod 54, which facilitates the control of the plasma temperature inside the container 93, facilitates water bath heating, and provides high temperature control accuracy.

[0029] In this invention, the body 1 is connected to an adding mechanism 4, which includes a storage box 41. The storage box 41 is installed at one end of the body 1, and the adding tube 43 is installed on the storage box 41. A first water pump 42 is installed on the adding tube 43. A third electric push rod 44 is installed on the body 1. The plug end of the adding tube 43 is installed on the telescopic end of the third electric push rod 44, thereby facilitating the addition of buffer solution to the inside of the container 93.

[0030] In this invention, the end of the guide block 92 opposite to the mounting plate 62 is equipped with a connecting block 7, and a suction tube 38 is installed on the connecting block 7. A filter mechanism 8 is connected to the guide block 92. The filter mechanism 8 includes a plug-in block 81. The plug-in block 81 is inserted between the guide block 92 and the connecting block 7. The connecting tube 96 is connected to the suction tube 38 through the plug-in block 81, thereby facilitating the filtration of whole blood and thus facilitating the filtration of plasma.

[0031] In this invention, the plug block 81 is equipped with a blocking filter membrane 82, which is a 0.45 µm PES membrane, thereby restricting the passage of cells and playing a filtering role.

[0032] Example 3: Based on Example 2, a sampling mechanism 3 is rotatably connected to the connecting block 7. The sampling mechanism 3 includes a sampling block 31. The sampling block 31 is rotatably connected to the connecting block 7. An insertion tube 34 is slidably connected inside the sampling block 31. A second spring 39 is installed between the insertion tube 34 and the sampling block 31. One end of the suction tube 38 is installed on the insertion tube 34. A first electric push rod 32 is installed on the machine body 1. The telescopic end of the first electric push rod 32 abuts against one end of the insertion tube 34. The bottom end of the sampling block 31 is slidably connected to... A blocking baffle 35 is connected, and a first spring 36 is installed between the blocking baffle 35 and the sampling block 31. The blocking baffle 35 is provided with a through hole 37, through which the insertion tube 34 communicates with the outside. A second electric push rod 33 is installed on the machine body 1. The telescopic end of the second electric push rod 33 abuts against the blocking baffle 35. The machine body 1 is located below the insertion tube 34 and holds the sampling tube 2, thereby facilitating the extraction of blood through the insertion tube 34. The blocking baffle 35 effectively restricts blood dripping, thereby ensuring the safety of the test.

[0033] Working principle: When testing plasma, the mounting plate 62, guide block 92, plug-in block 81, and connecting block 7 are plugged together. Screws can be installed at the joints to ensure a secure connection. Filtering can be selected as needed, allowing for the selection of a suitable plug-in block 81. Without filtration, the selected plug-in block 81 does not contain a filter membrane 82. The entire assembly is inserted into the machine body 1. The sampling block 31 is bent downwards to fit snugly against the machine body 1, ensuring it is vertical. The sampling tube 2 containing whole blood is clamped onto the machine body 1. The second electric push rod 33 is activated, extending and retracting. The first electric push rod 33 extends into the sampling block 31, and its telescopic end pushes the blocking baffle 35. The blocking baffle 35 slides, the first spring 36 is compressed, the through hole 37 moves to the bottom end of the insertion tube 34, and the first electric push rod 32 is activated. The first electric push rod 32 extends and its telescopic end extends into the sampling block 31. The telescopic end of the first electric push rod 32 abuts against the insertion tube 34, thereby pushing the insertion tube 34 to slide downward. The second spring 39 is compressed, and the bottom end of the insertion tube 34 extends into the sampling tube 2, thereby facilitating blood extraction. After the blocking baffle 35 closes, blood spillage is effectively prevented, improving the safety of the test. Before blood is drawn, the fifth electric push rod 91 is activated, extending into the guide block 92 and pushing the piston 94. The third spring 95 extends, causing the piston 94 to move and expel the air from the receiving box 93. The fifth electric push rod 91 then retracts, disengaging from the piston 94. The third spring 95 retracts, pulling the piston 94 back to its original position, creating a negative pressure inside the receiving box 93. This negative pressure is then created inside the filter membrane 82 and the suction tube 38 via a one-way valve, drawing the blood from the sampling tube 2 into the insertion tube 34. The blood then enters the insertion block 81 and is filtered through the filter membrane 82 (0.45 μm). A µm PES membrane is used to restrict cell passage and filter out plasma. The plasma enters the container 93. When buffer needs to be added, the third electric push rod 44 is activated, inserting the addition tube 43 into the container 93. The first water pump 42 is activated, and the buffer from the storage box 41 enters the addition tube 43 and is forced into the container 93 through a one-way valve, thus mixing with the plasma for easy culture. The temperature-controlled heating water tank 51 is activated, heating the water inside to a suitable temperature. The fourth electric push rod 54 is activated, pushing the water tube 52 to connect with the guide block 92. The second water pump 53 is activated, and the warm water inside the temperature-controlled heating water tank 51 enters the guide block 92 through one end of the water tube 52, and then flows back into the temperature-controlled heating water tank 51 through the other water tube 52, thus facilitating water bath heating of the container 93, making the temperature more stable and facilitating culture or PCR amplification. The fifth electric actuator 91 is activated, which pushes the piston 94 to allow the cultured plasma to be forced into the mounting plate 62 through the one-way valve. The plasma contacts the fixed end of the chromatography paper 64, facilitating chromatography. The plasma can then be inspected using the laser detector 61. An external Raman spectrometer is used to analyze the signal detected by the laser detector 61. When increased chromatography accuracy is required, and the existing length of the chromatography paper 64 is insufficient for the testing conditions, the exposed end of the chromatography paper 64 will be used up. At this time, the lead screw 66 is connected to the output shaft of the motor 63, and the motor 63 is started. The motor 63 rotates, driving the lead screw 66 to rotate. The slider drives the pull roller 69 to move. Under the action of the guide roller 68, the pull roller 69 pulls the chromatography paper 64 to fold. During plasma chromatography, the folded area of ​​the chromatography paper 64 becomes longer and the number of folded ends increases. The take-up roller 67 is released and the spiral spring 65 is compressed, exposing the new end of the chromatography paper 64, thus ensuring the continuity of chromatography. The spiral spring 65 uses a small elastic force, so it is not easy to form a large pressure between the chromatography paper 64 and the guide roller 68, thus not affecting the chromatography effect. At the same time, parallel plastic strips are woven inside the chromatography paper 64 to ensure the strength of the chromatography paper 64 and avoid excessive compression.

[0034] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A tuberculosis detection device, comprising a body (1), wherein a laser detection mechanism (6) is connected to the body (1), characterized in that: The laser detection mechanism (6) includes a mounting plate (62), which is inserted into the body (1). A laser detector (61) is installed on the top of the mounting plate (62) of the body (1). Chromatography paper (64) is provided inside the mounting plate (62). A take-up roller (67) is rotatably connected to one end of the mounting plate (62). A spiral spring (65) is installed between the take-up roller (67) and the mounting plate (62). The chromatography paper (64) is wound on the take-up roller (67). The free end of the chromatography paper (64) is fixed on the mounting plate (62). A pull roller (69) is slidably connected to the mounting plate (62). The chromatography paper (64) is wound around the pull roller (69). A drive assembly is connected to the mounting plate (62). A flow guiding mechanism (9) is connected to the mounting plate (62). The flow guiding mechanism (9) includes a flow guiding block (92). One end of the mounting plate (62) is inserted into the flow guiding block (92). An elevated container (93) is installed inside the flow guiding block (92). Both ends of the container (93) are connected to connecting pipes (96). A one-way valve is installed on the connecting pipes (96). The container (93) is unidirectionally connected to the chromatography paper (64) end of the mounting plate (62) through the connecting pipes (96) and the one-way valve. A piston (94) is slidably connected inside the container (93). A pushing component is connected to the piston (94). A water pipe (52) is inserted into the flow guiding block (92). An adding pipe (43) is inserted into the container (93). A one-way valve is also installed at the end of the container (93) where it is inserted into the adding pipe (43).

2. The tuberculosis detection device according to claim 1, characterized in that, The pulling assembly includes a lead screw (66), which is rotatably connected to the mounting plate (62). A slider is threaded onto the lead screw (66), and one end of the pulling roller (69) is fixedly connected to the slider. A motor (63) is installed on the machine body (1), and one end of the lead screw (66) is inserted into the output shaft of the motor (63).

3. The tuberculosis detection device according to claim 2, characterized in that, The mounting plate (62) is equipped with a guide roller (68) near the free end of the chromatography paper (64), and the chromatography paper (64) is wound and connected to the guide roller (68).

4. The tuberculosis detection device according to claim 1, characterized in that, The pushing assembly includes a third spring (95), which is installed between the piston (94) and the receiving box (93). A fifth electric push rod (91) is installed on the body (1), and the telescopic end of the fifth electric push rod (91) abuts against the piston (94).

5. A tuberculosis detection device according to claim 1, characterized in that, The body (1) is connected to a temperature control mechanism (5), which includes a temperature-controlled heating water tank (51). A pair of water pipes (52) are provided, and the other end of the pair of water pipes (52) is installed on the temperature-controlled heating water tank (51). A second water pump (53) is installed on one of the water pipes (52). A fourth electric push rod (54) is installed on the body (1). The plug ends of the pair of water pipes (52) are fixed on the telescopic end of the fourth electric push rod (54).

6. A tuberculosis detection device according to claim 1, characterized in that, The body (1) is connected to an adding mechanism (4), which includes a storage box (41). The storage box (41) is installed at one end of the body (1). The adding tube (43) is installed on the storage box (41). A first water pump (42) is installed on the adding tube (43). A third electric push rod (44) is installed on the body (1). The plug end of the adding tube (43) is installed on the telescopic end of the third electric push rod (44).

7. A tuberculosis detection device according to claim 1, characterized in that, The guide block (92) is equipped with a connecting block (7) at one end away from the mounting plate (62). A suction tube (38) is installed on the connecting block (7). A filter mechanism (8) is connected to the guide block (92). The filter mechanism (8) includes a plug-in block (81). A plug-in block (81) is inserted between the guide block (92) and the connecting block (7). The connecting tube (96) is connected to the suction tube (38) through the plug-in block (81).

8. A tuberculosis detection device according to claim 7, characterized in that, The plug block (81) is equipped with a blocking filter membrane (82), which is a 0.45 µm PES membrane.

9. A tuberculosis detection device according to claim 7, characterized in that, The connecting block (7) is rotatably connected to a sampling mechanism (3), the sampling mechanism (3) includes a sampling block (31), the connecting block (7) is rotatably connected to the sampling block (31), the sampling block (31) is slidably connected to an insertion tube (34), a second spring (39) is installed between the insertion tube (34) and the sampling block (31), one end of the suction tube (38) is installed on the insertion tube (34), a first electric push rod (32) is installed on the body (1), and the telescopic end of the first electric push rod (32) is connected to the insertion tube (34). One end of the sampling block (31) is in contact with the bottom end of the sampling block (31), and a blocking baffle (35) is slidably connected to it. A first spring (36) is installed between the blocking baffle (35) and the sampling block (31). A through hole (37) is provided on the blocking baffle (35). The insertion tube (34) is connected to the outside through the through hole (37). A second electric push rod (33) is installed on the body (1). The telescopic end of the second electric push rod (33) is in contact with the blocking baffle (35). The body (1) is located below the insertion tube (34) and holds the sampling tube (2).

10. A method of using a tuberculosis detection device according to any one of claims 1-9, characterized in that, Includes the following steps: S1. When testing plasma, insert the mounting plate (62), the guide block (92), the plug block (81) and the connecting block (7) together, bend the sampling block (31) downwards so that the sampling block (31) fits against the body (1), start the second electric push rod (33) to push the blocking baffle (35) to slide, the through hole (37) moves to the bottom of the insertion tube (34), start the first electric push rod (32) to push the insertion tube (34) to slide downwards, and the bottom of the insertion tube (34) extends into the sampling tube (2); S2. Before drawing blood, start the fifth electric push rod (91), the piston (94) moves to expel the air inside the container (93), the fifth electric push rod (91) retracts, the piston (94) returns to its original position, the blood in the sampling tube (2) is drawn into the container (93), start the first water pump (42), the buffer solution inside the container (41) is pressed into the container (93) through the addition tube (43), the buffer solution is mixed with the plasma, start the second water pump (53), the warm water inside the temperature-controlled heating water tank (51) enters the guide block (92) through the water pipe (52) to heat the container (93) in a water bath; S3. Start the fifth electric push rod (91), the piston (94) pushes the plasma into the interior of the mounting plate (62), the plasma contacts the fixed end of the chromatography paper (64) and performs chromatography. The plasma is checked by the laser detector (61). Start the motor (63), the motor (63) rotates, the drive screw (66) rotates, and the pull roller (69) moves through the slider. The pull roller (69) pulls the chromatography paper (64) to fold, the take-up roller (67) is released, the spiral spring (65) is compressed, the end of the new chromatography paper (64) is exposed, and the chromatography continues.

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