A plant regulator composition containing zearalenone and triacontanol and its use

Abstract

The application provides a plant regulator composition containing zearalenone and melengestrol and application thereof, the composition comprises a first active component and a second active component, the first active component is zearalenone, the second active component is melengestrol, and the mass ratio of the first active component to the second active component is 0.007-145:1.The zearalenone and melengestrol are compounded as the plant regulator for the first time, compared with single components, the zearalenone and melengestrol show good synergistic effect in improving the plant height, effective ear number, seed setting rate, thousand-grain weight, yield and the like of wheat, and the zearalenone and melengestrol are green and environmentally friendly, easy to be decomposed and metabolized by plants, can reduce the residual amount of pesticides on crops, improve the quality and safety level of agricultural products, provide a new candidate scheme for plant regulator compounding technology, and have wide application prospect.

Description

Technical Field

[0001] This invention belongs to the field of pesticide compound technology, and in particular relates to a plant growth regulator composition containing oryzanol and triacontanol and its application. Background Technology

[0002] Oryzanol is a novel plant growth regulator developed by Northeast Agricultural University. It is a nucleoside compound produced by the fermentation of NEAU6, an endophytic actinomycete of the Chinese medicinal herb Paris polyphylla. It has the effects of promoting rooting, germination and increasing yield in crops such as rice and wheat.

[0003] Triacontanol is a natural long-chain substance widely found in beeswax and plant waxes. Its pure form is a white, flaky crystal, poorly soluble in water and common organic solvents. Triacontanol can be used as a plant growth regulator with high bioavailability. It can be applied to crops such as citrus, wheat, rice, corn, peanuts, potatoes, apples, tea, lychees, grapes, soybeans, oyster mushrooms, jujubes, cotton, tobacco, tomatoes, mangoes, prickly ash, and sorghum, exhibiting functions such as yield increase, growth regulation, and stress resistance.

[0004] Oryzanol and triacontanol have been marketed as plant growth regulators, but mostly as single-agent applications. For example, invention patent CN111685126A discloses a plant growth regulator with oryzanol as the active ingredient, demonstrating that oryzanol aqueous solution can increase plant height, seedling fresh weight, and root fresh weight in rice. The "Handbook of Scientific Application of Pesticides in Crops (2017 New Edition)" (Zhongyuan Farmers Publishing House, February 2018) discloses that triacontanol can be used on cereal crops such as corn, wheat, and rice. Spraying at the beginning of heading can promote the transport of photosynthetic products to the panicle, increasing the number of grains per panicle and improving the thousand-grain weight.

[0005] Chinese invention patent CN107615939A discloses a method for breaking the dormancy of *Aristolochia debilis* seeds. The method involves soaking disinfected seeds in a mixture of 6-benzyladenine, triacontanol, and brassinolide, followed by soaking in a seed soaking solution containing oryzanol (vitamin Oxyphenidyl), which promotes dormancy breaking and increases seed germination rate. Although the above technical solution uses oryzanol and triacontanol together, they are used sequentially, not mixed.

[0006] There are no existing reports on the combination of oryzanol and triacontanol as a plant growth regulator, and the combined effect of the two and their application in plants have not been studied. Summary of the Invention

[0007] In view of this, the present invention aims to propose a plant growth regulator composition containing oryzanol and triacontanol and its application, demonstrating the synergistic effect between oryzanol and triacontanol, which can effectively promote wheat growth, increase yield, and broaden the application scenarios of oryzanol and triacontanol.

[0008] To achieve the above objectives, the technical solution of the present invention is implemented as follows: In a first aspect, the present invention provides a plant growth regulator composition, the composition comprising a first active component and a second active component, wherein the first active component is oryzanol and the second active component is triacontanol.

[0009] Further, the mass ratio of the first active component to the second active component is 0.007~145:1, for example, it can be 145:1, 116:1, 87:1, 58:1, 29:1, 20:1, 16:1, 14.5:1, 12:1, 10:1, 8:1, 7.25:1, 6:1, 5:1, 4:1, 3:1, 2.5:1, 2:1, 1.67:1, 1.5:1, 1.33:1, 1.25:1, 1:1, 0.8:1, 0.75:1, 0. The ratios are 67:1, 0.6:1, 0.5:1, 0.4:1, 0.33:1, 0.25:1, 0.2:1, 0.17:1, 0.125:1, 0.1:1, 0.08:1, 0.0625:1, 0.05:1, 0.03:1, 0.017:1, 0.011:1, 0.009:1, 0.007:1; preferably 0.05~116:1; more preferably 0.22~116:1, 0.05~4.53:1 or 0.22~4.53:1.

[0010] Further, the total mass percentage of the first active component and the second active component in the composition is 0.1% to 12.0%, for example, it can be 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, or 12%; preferably 1% to 10%.

[0011] Furthermore, the composition further includes at least one of a dispersant, an emulsifier, an antifreeze, a wetting agent, a disintegrant, and a filler.

[0012] Further, the dosage form of the composition is a soluble concentrate, a suspension concentrate, a wettable powder, a soluble powder, a water-dispersible granule, a soluble granule, an emulsifiable concentrate, a water emulsion, or a microemulsion; preferably a water-dispersible granule.

[0013] When formulating water-dispersible granules, the adjuvants selected by those skilled in the art may include, for example: dispersants such as one or more of polycarboxylate, lignin sulfonate, and alkyl naphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, and naphthalene sulfonate; disintegrants such as one or more of ammonium sulfate, urea, sucrose, and glucose; binders such as one or more of diatomaceous earth, corn starch, PVA, and carboxymethyl (ethyl) cellulose; and fillers such as one or more of diatomaceous earth, kaolin, silica, light calcium carbonate, talc, attapulgite, and clay.

[0014] When formulating soluble or suspending agents, those skilled in the art are well aware of the use of appropriate additives to complete this invention. Examples of such additives include: dispersants such as one or more of polycarboxylate, lignin sulfonate, and alkylnaphthalene sulfonate; emulsifiers such as calcium dodecylbenzenesulfonate (Agricultural Emulsion 500#), Agricultural Emulsion 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Agricultural Emulsion 2201#, Span-60# (common name: sorbitan stearate), and Tween-60# (common name: ...). One or more of the following: polyoxyethylene sorbitan stearate, TX-10 (generic name: octylphenol polyoxyethylene (10) ether), AEO-9 (fatty alcohol polyoxyethylene ether), agricultural emulsion 1601# (generic name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), agricultural emulsion 600#, and agricultural emulsion 400#; antifreeze such as glycerol, ethylene glycol, etc.; solvents such as deionized water, methanol, dimethylformamide, N-methylpyrrolidone, cyclohexanone, acetone, and dimethyl sulfoxide.

[0015] When formulating wettable powders, soluble powders, or soluble granules, the adjuvants selected by those skilled in the art may include, for example, dispersants such as one or more of polycarboxylate, lignin sulfonate, and alkyl naphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, and naphthalene sulfonate; and fillers such as one or more of ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, kaolin, silica, light calcium carbonate, talc, attapulgite, and clay.

[0016] When formulating emulsifiable concentrates, water-based emulsions, or microemulsions, adjuvants selected by those skilled in the art may include, for example, emulsifiers such as calcium dodecylbenzenesulfonate (Agricultural Emulsion 500#), Agricultural Emulsion 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Agricultural Emulsion 2201#, Span-60# (common name: dehydrated sorbitan stearate), Tween-60# (common name: polyoxyethylene dehydrated sorbitan stearate), Agricultural Emulsion 1601# (common name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), Agricultural Emulsion 600#, and Agricultural Emulsion 400#; and solvents such as deionized water, methanol, dimethylformamide, N-methylpyrrolidone, cyclohexanone, acetone, and dimethyl sulfoxide. Furthermore, this invention also provides the application of the aforementioned plant growth regulator composition containing oryzanol and triacontanol in improving plant growth and development.

[0017] In a second aspect, the present invention provides the use of the plant growth regulator composition described in the first aspect in promoting wheat growth.

[0018] Furthermore, the application includes at least one of the following: (1) Application in improving the height of wheat plants; (2) Application in increasing the number of effective ears of wheat; (3) Application in improving wheat seed setting rate; (4) Application in increasing the thousand-grain weight of wheat; (5) Application in increasing wheat yield.

[0019] Further, the mass ratio of the first active component to the second active component is 0.007~145:1, for example, it can be 145:1, 116:1, 87:1, 58:1, 29:1, 20:1, 16:1, 14.5:1, 12:1, 10:1, 8:1, 7.25:1, 6:1, 5:1, 4:1, 3:1, 2.5:1, 2:1, 1.67:1, 1.5:1, 1.33:1, 1.25:1, 1:1, 0.8:1, 0.75:1, 0. The ratios are 67:1, 0.6:1, 0.5:1, 0.4:1, 0.33:1, 0.25:1, 0.2:1, 0.17:1, 0.125:1, 0.1:1, 0.08:1, 0.0625:1, 0.05:1, 0.03:1, 0.017:1, 0.011:1, 0.009:1, 0.007:1; preferably 0.05~116:1; more preferably 0.22~116:1, 0.05~4.53:1 or 0.22~4.53:1.

[0020] Further, the plant growth regulator composition is provided in solution form, wherein the concentration of the first active component is 0.1 mg / L to 14.5 mg / L, for example, it can be 0.1 mg / L, 0.5 mg / L, 0.7 mg / L, 1 mg / L, 1.5 mg / L, 2 mg / L, 2.9 mg / L, 3 mg / L, 4 mg / L, 5 mg / L, 5.8 mg / L, 6 mg / L, 7 mg / L, 8 mg / L, 8.7 mg / L, 9 mg / L, 10 mg / L, 11 mg / L, 11.6 mg / L, 12 mg / L, 13 mg / L, 13.135 mg / L, 14 mg / L, etc. The concentration of the second active component is 0.1 mg / L to 14.5 mg / L, for example, it can be 0.1 mg / L, 0.5 mg / L, 0.7 mg / L, 1 mg / L, 1.5 mg / L, 2 mg / L, 2.9 mg / L, 3 mg / L, 4 mg / L, 5 mg / L, 5.8 mg / L, 6 mg / L, 7 mg / L, 8 mg / L, 8.7 mg / L, 9 mg / L, 10 mg / L, 11 mg / L, 11.6 mg / L, 12 mg / L, 12.698 mg / L, 13 mg / L, 14 mg / L, or 14.5 mg / L. Preferably, the concentration of the first active component is 0.7 mg / L to 14 mg / L, and the concentration of the second active component is 0.1 mg / L to 14 mg / L; More preferably, the concentration of the first active component is 0.7 mg / L to 13.135 mg / L, 2.9 mg / L to 14 mg / L, or 2.9 mg / L to 13.135 mg / L, and the concentration of the second active component is 0.1 mg / L to 12.698 mg / L, 2.9 mg / L to 14 mg / L, or 2.9 mg / L to 12.698 mg / L.

[0021] Thirdly, the present invention provides a method for promoting wheat growth, the method comprising the following steps: applying a plant regulator composition containing a first active component and a second active component to wheat leaves, wherein the first active component includes oryzanol and the second active component includes triacontanol.

[0022] Further, the mass ratio of the first active component to the second active component is 0.007~145:1, for example, it can be 145:1, 116:1, 87:1, 58:1, 29:1, 20:1, 16:1, 14.5:1, 12:1, 10:1, 8:1, 7.25:1, 6:1, 5:1, 4:1, 3:1, 2.5:1, 2:1, 1.67:1, 1.5:1, 1.33:1, 1.25:1, 1:1, 0.8:1, 0.75:1, 0. The ratios are 67:1, 0.6:1, 0.5:1, 0.4:1, 0.33:1, 0.25:1, 0.2:1, 0.17:1, 0.125:1, 0.1:1, 0.08:1, 0.0625:1, 0.05:1, 0.03:1, 0.017:1, 0.011:1, 0.009:1, 0.007:1; preferably 0.05~116:1; more preferably 0.22~116:1, 0.05~4.53:1 or 0.22~4.53:1.

[0023] Further, the plant growth regulator composition is provided in solution form, wherein the concentration of the first active component is 0.1 mg / L to 14.5 mg / L, for example, it can be 0.1 mg / L, 0.5 mg / L, 0.7 mg / L, 1 mg / L, 1.5 mg / L, 2 mg / L, 2.9 mg / L, 3 mg / L, 4 mg / L, 5 mg / L, 5.8 mg / L, 6 mg / L, 7 mg / L, 8 mg / L, 8.7 mg / L, 9 mg / L, 10 mg / L, 11 mg / L, 11.6 mg / L, 12 mg / L, 13 mg / L, 14 mg / L, etc. The concentration of the second active component is 0.1 mg / L to 14.5 mg / L, for example, it can be 0.1 mg / L, 0.5 mg / L, 0.7 mg / L, 1 mg / L, 1.5 mg / L, 2 mg / L, 2.9 mg / L, 3 mg / L, 4 mg / L, 5 mg / L, 5.8 mg / L, 6 mg / L, 7 mg / L, 8 mg / L, 8.7 mg / L, 9 mg / L, 10 mg / L, 11 mg / L, 11.6 mg / L, 12 mg / L, 13 mg / L, 14 mg / L, or 14.5 mg / L. Preferably, the concentration of the first active component is 0.7 mg / L to 14 mg / L, and the concentration of the second active component is 0.1 mg / L to 14 mg / L; More preferably, the concentration of the first active component is 0.7 mg / L to 13.135 mg / L, 2.9 mg / L to 14 mg / L, or 2.9 mg / L to 13.135 mg / L, and the concentration of the second active component is 0.1 mg / L to 12.698 mg / L, 2.9 mg / L to 14 mg / L, or 2.9 mg / L to 12.698 mg / L.

[0024] Furthermore, the plant growth regulator composition is sprayed once each during the wheat's pre-winter tillering stage, the wheat's heading stage, and the wheat's flowering stage.

[0025] Compared with the prior art, the plant growth regulator composition containing oryzanol and triacontanol described in this invention and its application have the following advantages: (1) This invention is the first to combine oryzanol and triacontanol as a plant growth regulator. Compared with the single component, it shows a good synergistic effect in improving wheat plant height, effective spike number, seed setting rate, thousand-grain weight and yield. It expands the application scope of oryzanol and triacontanol in the field of plant growth regulators, and also provides a new candidate scheme for the compounding technology of plant growth regulators, which has broad application prospects. (2) Both oryzanol and triacontanol of the present invention are low-toxicity plant growth regulators. They are green and environmentally friendly, easily decomposed and metabolized by plants, have low toxicity to humans and animals, and have no irritating effect on the skin. When the two are combined, they can significantly reduce the amount used per unit area while still maintaining excellent regulatory effect, which can reduce the amount of pesticide residues on crops and improve the quality and safety level of agricultural products. Attached Figure Description

[0026] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings: Figure 1 The equivalent curve for regulating wheat yield using a combination of oryzanol and triacontanol. Detailed Implementation

[0027] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0028] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0029] Example 1

[0030] The method for preparing the plant growth regulator composition (2.9% oryzanol, 0.1% triacontanol water-dispersible granules) in this embodiment is as follows: Formula composition: 2.9% oryzanol, 0.1% triacontanol, 4% D350, 8% D325, 2% DW205, 10% bentonite, ammonium sulfate to make up to 100%. The above materials are added to a twin-screw mixer in the corresponding proportions and mixed evenly. Then, they are pulverized to 800 mesh using an air jet mill. The pulverized material is then mixed evenly with 12% deionized water by weight, granulated by extrusion, and sieved to produce water-dispersible granules with an effective ingredient content of 3% oryzanol and triacontanol.

[0031] Comparative Example 1 The difference between this comparative example and Example 1 is that no oryzanol is added, while the remaining steps are the same as in Example 1.

[0032] Comparative Example 2 The difference between this comparative example and Example 1 is that triacontanol is not added, while the remaining steps are the same as in Example 1.

[0033] Comparative Example 3 The difference between this comparative example and Example 1 is that oryzanol is replaced with an equal amount of furfural, while the remaining steps are the same as in Example 1.

[0034] Comparative Example 4 The difference between this comparative example and Example 1 is that triacontanol is replaced with an equal amount of benzylaminopurine, while the remaining steps are the same as in Example 1.

[0035] Test Example 1 The plant growth regulator compositions prepared in Example 1 and Comparative Examples 1-4 were subjected to efficacy tests, as detailed below: 1. Test target: Wheat (Triticum aestivum L.), variety Zhenmai 18. Wheat seeds were purchased from the market.

[0036] 2. How to use: spray.

[0037] 3. Test basis: GB / T 17980.132-2004 "Guidelines for Field Efficacy Tests of Pesticides (II)" Part 132: Wheat Growth Regulator Tests; 4. Experimental material cultivation Rotary tillage was carried out before sowing on November 8, 2024, and mechanical application was carried out on November 9, 2024, with a seed rate of 22.5 kg per mu. The cultivation and management practices were in line with local agricultural production practices. Experiments were conducted on December 14, 2024, during the wheat's pre-winter tillering stage; March 30, 2025, during the wheat's booting stage; and April 8, 2025, during the wheat's flowering stage.

[0038] 5. Chemical treatment The test material was sprayed with a 3WBD-16A electric sprayer with a conical nozzle, diluted 2500-3000 times. The pesticide was applied once each during the wheat's pre-winter, heading, and flowering stages. A blank control was treated first, followed by the pesticide treatment. The sprayer was rinsed three times with clean water before each application of a different pesticide.

[0039] 6. Observation and Results Statistics: Ten wheat plants with uniform growth were marked before the application of the pesticide. Yield was measured on May 28, 2025, the wheat harvest season.

[0040] Yield measurement: Five random sampling points were taken from each plot, with each point covering 0.2 square meters. The wheat yield was measured after mixing, threshing, and drying.

[0041] Calculation formula

[0042] The indicator in the formula is output.

[0043] The results are shown in Table 1. In Example 1, the oryzanol-triacontanol composition prepared by compounding oryzanol and triacontanol achieved the highest number of ears per mu, number of grains per ear, ear length, thousand-grain weight, and yield, with the highest yield increase rate. This proves that the plant regulator composition of the present invention can effectively promote wheat growth, increase yield, and is less likely to cause adverse phenomena such as excessively short ear length, insufficient number of grains per ear, and excessively low number of ears per mu.

[0044] Compared to Example 1, Comparative Example 1 or Comparative Example 2 lacked one of the active components, resulting in a significant reduction in the wheat growth-promoting effect. This demonstrates that the compound composition of the present invention has a better plant regulation effect than a single component. The combination of oryzanol and triacontanol is not a simple additive effect, but rather a significant improvement in the wheat growth-promoting effect through the complementarity and synergy of different mechanisms of action.

[0045] Compared with Example 1, Comparative Examples 3 and 4 replaced one of the active components, and their promoting effect on wheat growth was also weaker than that of Example 1. This proves that the combination of two specific active components selected in this invention has unique advantages in promoting the coordination of vegetative and reproductive growth compared with other conventional plant growth regulators.

[0046] Test Example 2 In Test Example 2, the same proportions of raw materials as in Example 1 were used, and the test method was the same as in Test Example 1. The difference was that oryzanol was sprayed first, followed by the composition of other raw materials. The effect on promoting wheat growth was also weaker than that in Example 1 of Test Example 1. This proves that the compound composition of the present invention has a better plant regulation effect than the separate sequential application of single components, and has a unique advantage in promoting the coordination of vegetative growth and reproductive growth.

[0047] Table 1. Wheat growth status in the examples and comparative cases

[0048] Example 2

[0049] Weigh a certain amount of oryzanol technical grade, dissolve it in water to form an oryzanol stock solution; weigh triacontanol technical grade, add 100 ml of 95% ethanol and 1 g of Tween-80 emulsifier, add to a beaker, and slowly heat while stirring. When the oil phase temperature rises to 75-85℃, continue stirring until the oil phase is completely dissolved and transparent. Adjust the volume to 1000 ml with hot water above 90℃ to prepare a 1 g / L triacontanol stock solution for later use. First, dilute the single-agent stock solution with 0.1% Tween-80 water to the concentration gradient designed in Table 2, then mix them in pairs according to the same volume. A blank control was set up containing only ethanol and Tween-80 and no active ingredient.

[0050] Table 2. Pharmaceutical Experimental Design

[0051] The efficacy of each group in Table 2 was tested, and the specific methods are as follows: 1. Test target: Wheat (Triticum aestivum L.), variety Zhenmai 18. Wheat seeds were purchased from the market.

[0052] 2. Usage method: Spray 3. Test basis: GB / T 17980.132-2004 "Guidelines for Field Efficacy Tests of Pesticides (II)" Part 132: Wheat Growth Regulator Tests; NY / T 2061.5-2016 "Guidelines for Indoor Bioassay Tests of Pesticides: Plant Growth Regulators" Part 5: Determination of Combined Effects of Mixtures.

[0053] 4. Experimental material cultivation Rotary tillage was carried out before sowing on November 8, 2024, and mechanical application was carried out on November 9, 2024, with a seed rate of 22.5 kg per mu. The cultivation and management practices were in line with local agricultural production practices. Experiments were conducted on December 14, 2024, during the wheat's pre-winter tillering stage; March 30, 2025, during the wheat's booting stage; and April 8, 2025, during the wheat's flowering stage.

[0054] 5. Chemical treatment The test materials were sprayed using a 3WBD-16A electric sprayer with a conical nozzle. The pesticide was applied once each during the pre-winter wheat growth stage, the wheat heading stage, and the poplar flowering stage. A blank control was treated first, followed by treatments with the test pesticide in order of increasing concentration. Each treatment was marked immediately after completion. The sprayer was rinsed three times with clean water before applying a different pesticide.

[0055] 6. Dosage and composition design A semi-field trial was conducted to screen the optimal ratio of oryzanol and triacontanol for regulating wheat growth. The test materials were sprayed using a 3WBD-16A electric sprayer with a conical nozzle. The pesticide was applied once each during the wheat's pre-winter tillering stage, the wheat's heading stage, and the flowering stage.

[0056] 7. Observation and Results Statistics: Ten wheat plants with uniform growth were marked before the pesticide was applied. Yield was measured on May 28, 2025, the wheat harvest season.

[0057] Yield measurement: Five random sampling points were taken from each plot, with each point covering 0.2 square meters. The wheat yield was measured after mixing, threshing, and drying.

[0058] Calculation formula

[0059] The indicator in the formula is output.

[0060] Statistical analysis: The combined effects of oryzanol and triacontanol on wheat yield were evaluated using the isoline method. Regression analysis was performed on the results, and the toxicity equation and effect-adjusting factor (ED) were derived using DPS software. 15 Values. The horizontal and vertical axes represent the concentrations of single-agent triacontanol and oryzanol, respectively. The ED values ​​of the corresponding single agents are plotted on both axes. 15 Connecting the points on the graph yields the theoretical equivalence line for the combination of oryzanol and triacontanol. Then, plot the ED values ​​of each combination on the graph. 15 The values ​​are calculated, and edge points are removed and connected to form a line, resulting in an equivalent line diagram of the oryzanol and triacontanol combination. Values ​​below the theoretical equivalent line indicate synergistic effects, values ​​above the theoretical equivalent line indicate antagonistic effects, and values ​​close to the theoretical equivalent line indicate additive effects.

[0061] 8. Results and Analysis Table 3. Combined effect of oryzanol and triacontanol on wheat yield

[0062] Table 4 Regression analysis of yield regulation by the combination of oryzanol and triacontanol

[0063] As shown in Table 3, all compound combinations exhibited higher yield-promoting rates than their respective single-agent counterparts, and the isotropic effect was consistently demonstrated in all cases. This result fully demonstrates that the synergistic effect of oryzanol and triacontanol has broad dosage adaptability, providing ample room for adjustment in the formulation design of field preparations.

[0064] As shown in Table 4, the correlation coefficient (R) of the toxicity regression equation for each treatment group was greater than 0.9, indicating a good fit of the regression model and ED. 15The estimated values ​​are reliable. The toxicity regression slopes and intercepts of oryzanol and triacontanol at different fixed doses show clear patterns, providing a solid mathematical basis for drawing equivalence diagrams and determining their combined effects.

[0065] according to Figure 1 The isoequivalence plot results show that the measured isoequivalence lines for adjusting yield of the oryzanol and triacontanol combination are generally below the theoretical isoequivalence lines, confirming that the combination exhibits a synergistic effect in yield regulation. Furthermore, the synergistic effect is most pronounced between the coordinates of the two isoequivalence lines (2.9, 13.135) and (12.698, 2.9), i.e., when triacontanol is quantified at 2.9 mg / L, and oryzanol ED... 15 The concentration was 13.135 mg / L, oryzanol was 2.9 mg / L (quantitative), and triacontanol ED... 15 The optimal synergistic effect was achieved at a concentration of 12.698 mg / L. This demonstrates that the regulator composition of the present invention can achieve excellent regulatory effects at lower active ingredient dosages, effectively reducing the amount of plant regulators required.

[0066] In conclusion, the combination of oryzanol and triacontanol can improve wheat yield to a certain extent, showing a synergistic effect without antagonistic effects, and can be used as a mixture.

[0067] Formulation Preparation Example 1 The formulation of 3% oryzanol·triacontanol water-dispersible granules consists of: 2.9% oryzanol, 0.1% triacontanol, 4% D350, 8% D325, 2% DW205, 10% bentonite, and ammonium sulfate to make up to 100%. The above materials are added to a twin-screw mixer in the corresponding proportions and mixed evenly. Then, the mixture is pulverized to 800 mesh using an air jet mill. The pulverized material is then mixed evenly with 12% deionized water by its weight, granulated by extrusion, and sieved to produce water-dispersible granules with an effective ingredient content of 3% oryzanol·triacontanol.

[0068] Formulation Preparation Example 2 The formulation of a 3% oryzanol-triacontanol water-based emulsion consists of: 2.9% oryzanol, 0.1% triacontanol, 4% fatty alcohol polyoxyethylene ether, 8% castor oil polyoxyethylene ether, 8% xylene, and purified water to make up to 100%. The above materials are subjected to high-speed shearing in a mixing tank according to the corresponding proportions to obtain a 3% oryzanol-triacontanol microemulsion.

[0069] Formulation Preparation Example 3 The formulation of 3% oryzanol·triacontanol wettable powder consists of: 2.9% oryzanol, 0.1% triacontanol, 8% sodium sulfate, 5% alkyl sulfate, 3% sodium lignosulfonate, 5% silica, 4% diatomaceous earth, and kaolin to 100%. The above materials are added to a twin-screw mixer in the corresponding proportions, mixed evenly, and then pulverized to 500 mesh using an air jet mill to produce a 3% oryzanol·triacontanol wettable powder.

[0070] The embodiments described above are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

Claims

1. A plant growth regulator composition, characterized in that: The composition comprises a first active component and a second active component, wherein the first active component is oryzanol and the second active component is triacontanol, and the mass ratio of the first active component to the second active component is 0.007~145:

1.

2. The plant growth regulator composition according to claim 1, characterized in that: The mass ratio of the first active component to the second active component is 0.05 to 116:

1.

3. The plant growth regulator composition according to claim 1, characterized in that: The composition further includes at least one of a dispersant, emulsifier, antifreeze, wetting agent, disintegrant, and filler.

4. The plant growth regulator composition according to claim 1, characterized in that: The dosage form of the composition is a soluble concentrate, suspension concentrate, wettable powder, soluble powder, water-dispersible granule, soluble granule, emulsifiable concentrate, water emulsion, or microemulsion; preferably, it is a water-dispersible granule.

5. The plant growth regulator composition according to claim 1, characterized in that: The total mass percentage of the first active component and the second active component in the composition is 0.1% to 12.0%; preferably 1% to 10%.

6. The use of the plant growth regulator composition according to any one of claims 1-5 in promoting wheat growth.

7. The application according to claim 6, characterized in that, The application includes at least one of the following: (1) Application in improving the height of wheat plants; (2) Application in increasing the number of effective ears of wheat; (3) Application in improving wheat seed setting rate; (4) Application in increasing the thousand-grain weight of wheat; (5) Application in increasing wheat yield.

8. A method for promoting wheat growth, characterized in that, The method includes the following steps: applying a plant growth regulator composition containing a first active component and a second active component to wheat leaves, wherein the first active component includes oryzanol, the second active component includes triacontanol, and the mass ratio of the first active component to the second active component is 0.007~145:

1.

9. The method according to claim 8, characterized in that, The mass ratio of the first active component to the second active component is 0.05 to 116:

1.

10. The method according to claim 8, characterized in that, The plant growth regulator composition is provided in solution form, wherein the concentration of the first active component is 0.1 mg / L to 14.5 mg / L, and the concentration of the second active component is 0.1 mg / L to 14.5 mg / L; Preferably, the concentration of the first active component is 0.7 mg / L to 14 mg / L, and the concentration of the second active component is 0.1 mg / L to 14 mg / L.

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