An immune cell protein polypeptide freeze-dried powdering device

Abstract

This invention provides a freeze-drying and pulverizing device for immune cell protein polypeptides, relating to the field of biopharmaceutical preparation technology. It includes: a mounting frame with a pipe assembly at its top; sieving components installed at both ends of the pipe assembly; the sieving components connected to both ends of the pipe assembly; a first motor driving a first gear to rotate and mesh with first toothed rings on both sides, causing two freezing tubes to rotate, improving temperature uniformity of the internal solution under low-temperature conditions; a connecting ring that does not rotate with the freezing tubes but is internally hollow, allowing the inlet pipe to feed the solution from the top and into the freezing tube; a cooling pipe surrounding the freezing tubes to cool them; a temperature sensor installed on the inner wall of the freezing tubes to constantly monitor the internal temperature; and baffles at both ends of the freezing tubes and the connected sieving components that can be used alternately, alternately feeding powder from both ends during each movement, improving processing efficiency.

Description

Technical Field

[0001] This invention relates to the field of biopharmaceutical preparation technology, specifically to an apparatus for freeze-drying and pulverizing immune cell protein polypeptides. Background Technology

[0002] "Immune cell protein peptide freeze-drying powdering" refers to the process of extracting proteins or peptides from immune cells, followed by enzymatic hydrolysis and freeze-drying (lyophilization) to produce stable, easily soluble, and highly active powder formulations for enhancing immune regulation or serving as raw materials for biological agents. Enzymatic hydrolysis breaks down large protein molecules into smaller peptide molecules, improving intestinal or cellular absorption. Freeze-drying (pre-freezing to below -40°C and vacuum sublimation) dehydrates the protein at low temperatures, preserving its biological activity and preventing thermal denaturation, ultimately yielding a loose powder with a moisture content ≤3%. This powder is stable at room temperature for short periods but requires long-term refrigeration at -20°C. It requires the addition of preservatives (such as trehalose or mannitol), control of the cooling rate (1-2°C / min), prevention of collapse, and aseptic / clean packaging to prevent oxidation or microbial contamination.

[0003] In existing technologies, the preparation of immune cell protein peptides requires freezing, crushing, and pulverizing and sieving processes, but this process involves transportation, resulting in low production efficiency. Summary of the Invention

[0004] To address the shortcomings of existing technologies, the present invention aims to provide an apparatus for freeze-drying and pulverizing immune cell protein peptides to solve the problems mentioned in the background section. To achieve the above objectives, the present invention provides the following technical solution: an immune cell protein polypeptide freeze-drying and pulverizing device, comprising: a mounting frame, a pipe assembly at the top of the mounting frame, sieving components mounted at both ends of the pipe assembly, the sieving components communicating with both ends of the pipe assembly, and a driving component disposed inside the pipe assembly; the pipe assembly includes freezing tubes, two sets of freezing tubes, top frames rotatably mounted at both ends of the two sets of freezing tubes, and connecting rings rotatably connected to the outer surfaces of the two ends of the freezing tubes near the top frames, the connecting rings being fixedly connected to the top frames, and the connecting rings... An inlet pipe is fixed at the top of the refrigeration tube, and the inlet pipe communicates with the inside of the refrigeration tube through a connecting ring; the driving assembly includes a moving plate, which is slidably installed inside the refrigeration tube, and a ring rope is sleeved inside the refrigeration tube. The two ends of the ring rope slide out from the top frame, and the moving plate is fixed on the surface of the ring rope located inside the refrigeration tube; the sieving assembly includes a bottom frame, which is fixed on the mounting bracket, and a lower filter frame is rotatably installed inside the bottom frame. An upper filter frame is snapped onto the top of the lower filter frame, and the upper and lower filter frames are combined into a hollow cylinder, with filter holes evenly spaced on the surfaces of the upper and lower filter frames.

[0005] Furthermore, the piping assembly also includes: baffles, insert rods, and return springs. Baffles are slidably inserted into both ends of the freezing tube. An insert rod is fixed to one end of the baffle facing the top frame, and the insert rod slides out of the top frame. A return spring is sleeved on the surface of the end of the insert rod, and the other end of the return spring is fixedly connected to the top frame. The freezing tube is fitted with a refrigeration tube on the outer surface of the two connecting rings, and the refrigeration tube is fixed on the mounting bracket. A vacuum pump is mounted on the surface of the mounting bracket and is fixedly connected to the bottom of the connecting rings.

[0006] Furthermore, a first toothed ring is fixedly sleeved at one end of the freezing tube and the rotatable connection of the top frame, and a first gear is rotatably mounted on the top frame between the two first toothed rings. The first gear meshes with the first toothed rings on both sides. A first motor is fixedly mounted on the top frame outside the first gear, and the output end of the first motor is fixedly connected to the first gear.

[0007] Furthermore, the drive assembly also includes: a fourth motor, a second screw, a moving frame, and a limiting frame. The second screw is rotatably mounted on the top of the two top frames. The fourth motor is fixed to the top of the top frame on one side of the second screw, and the output end of the fourth motor is fixedly connected to the second screw. The moving frame is threaded onto the surface of the second screw, and the two sides of the moving frame are fixedly connected to the loop rope on the outside of the freezing tube. The limiting frame is fixed at the position where the loop rope passes through, and the loop rope slides through the limiting frame. Multiple crushing cones are fixed in a ring on both sides of the moving plate.

[0008] Furthermore, the sieving assembly also includes: a receiving cylinder, a first discharge channel, an expansion layer, and a connecting frame. The two ends of the bottom frame are fixed with expansion layers, and the expansion layers are connected to the interior of the bottom frame. The bottom of the expansion layer is fixed with the first discharge channel. The top of the bottom frame is fixed with the connecting frame, and the connecting frame is fixedly connected to the bottom of the top frame. The top of the expansion layer on one side of the connecting frame and the bottom frame is fixed with a receiving cylinder. The upper filter frame slides into the receiving cylinder, opening the lower filter frame to receive powder.

[0009] Furthermore, the connecting frame is rotatably mounted with a second gear ring on the outside of the storage tube, the lower filter frame is fixedly connected to the second gear ring, one side of the second gear is meshed with a second gear, the bottom frame is fixed with a second motor corresponding to the top of the second gear, and the output end of the second motor is fixedly connected to the second gear; wherein, the top of both sides of the lower filter frame is provided with side grooves, and the bottom of both sides of the upper filter frame slides along the inside of the side grooves.

[0010] Furthermore, a first circular plate is fixed to one end of the upper filter frame facing the storage cylinder. The first circular plate slides along the inside of the storage cylinder. A collar is provided on the outer edge of the first circular plate. A first screw is rotatably installed on the top of the storage cylinder. The first circular plate is threaded onto the surface of the first screw through the collar. A motor that drives the first screw to rotate is fixed to the outer end of the storage cylinder.

[0011] Furthermore, an arc plate is slidably connected inside the bottom frame, and a bracket is fixed to the bottom of the collar of the arc plate and the first circular plate. The top and bottom of the bracket are slidably inserted into the storage tube and the expansion layer, respectively. The storage tube has a delivery outlet at the bottom of the expansion layer, and a second discharge channel is fixed at the bottom of the delivery outlet. Slider blocks are fixed to the top of both ends of the arc plate, and grooves are opened on both sides of the top of the bottom frame and the expansion layer, and the sliders slide along the inside of the grooves. The outlet end of the second discharge channel extends out of the expansion layer, and a sealing plate is rotatably installed at the outlet end of the second discharge channel.

[0012] Furthermore, a stirring frame is rotatably mounted on the first circular plate at the axis at the bottom of the upper filter frame. A third motor is fixed to the other end of the first circular plate, and the output end of the third motor is fixedly connected to the stirring frame. A second circular plate is inserted into the other end of the stirring frame, and the second circular plate slides along the inside of the lower filter frame.

[0013] Furthermore, a turntable is rotatably mounted on the surface of the second circular plate at the insertion end of the stirring frame, and electric push rods are fixed on both sides of the turntable. The extended ends of the electric push rods are fixed with insertion posts, and the shaft of the stirring frame is provided with insertion holes corresponding to the positions of the insertion posts on both sides.

[0014] The beneficial effects of this invention are: This invention uses a first motor to drive a first gear to rotate and mesh with first toothed rings on both sides, causing the two freezing tubes to rotate. This improves the temperature uniformity of the internal solution in a low-temperature environment. The connecting ring does not rotate with the freezing tubes, but its interior is hollow, allowing the inlet pipe to feed the solution from the top and into the interior of the freezing tubes. The freezing tubes are cooled by a refrigeration pipe fitted around them. A temperature sensor is installed on the inner wall of the freezing tubes to monitor the internal temperature at all times. The baffles at both ends of the freezing tubes and the connected sieving components can be used alternately, allowing the powder to be fed out alternately from both ends each time the tubes are moved, thus improving processing efficiency.

[0015] This invention uses a fourth motor to drive the second screw to rotate. The moving frame is threadedly engaged with the second screw, causing the outer sides of the two ring ropes to move. Consequently, the inner sides of the ring ropes move along the inside of the freezing tube. The moving plate moves along the inside of the freezing tube. Initially, the moving plate moves to one end of the freezing tube, and the immune cell protein polypeptide solution is fed in from the other end inlet pipe. After freezing, the raw materials inside the freezing tube are crushed by a crushing cone on the opposite side of the moving plate. When the moving plate moves to the side of the baffle, the baffle can be opened by squeezing, thereby sending the frozen raw materials into the sieving assembly. The movement of the second screw is reciprocating. After each movement, the mixture is fed in from the other end, thereby reducing the intermediate waiting time and improving production efficiency.

[0016] This invention uses a motor to drive a first screw to rotate, causing a first circular plate to move the upper filter frame along the top of the lower filter frame. The upper and lower filter frames slide together along the side groove. After the upper filter frame moves away, the frozen material inside the freezing tube is fed into the lower filter frame through the drive assembly. The first circular plate is connected to the first screw and the insert through the collar on its outer ring. When the upper and lower filter frames rotate, they are not interfered with by the first screw and the insert. After the upper and lower filter frames are combined, a second motor drives a second gear to mesh with a second gear ring, causing the upper and lower filter frames to rotate, thus accelerating the separation and screening of raw materials.

[0017] This invention pushes large, unscreened particles towards one side of the collection cylinder, where they are collected through the outlet and second discharge channel. As the first circular plate rotates with the first screw and is drawn into the collection cylinder, the inserter moves the arc plate inside the bottom frame, sending the fine powder screened by the lower and upper filter frames out through the first discharge channel on one side of the collection cylinder. When the first circular plate resets, the arc plate can move from one end of the bottom frame to the other, sending the powder out through the first discharge channel on the other side. This process involves the upper and lower filter frames merging and screening the raw materials, followed by the movement of the second circular plate to send out the powder. When the raw materials enter the lower filter frame, the insert of the electric pusher separates from the insert of the agitator, and the second circular plate remains stationary on the other side. The upper filter frame and the first circular plate move separately into the collection frame, where a third motor drives the agitator to rotate. After the raw materials enter the upper and lower filter frames, the agitator stirs the materials, thereby accelerating the screening process. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of this disclosure; Figure 2 This is a schematic diagram of the driving component structure in an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of the present disclosure; Figure 3This is a schematic diagram of the internal structure of the top frame in an embodiment of an immune cell protein polypeptide freeze-drying pulverization device. Figure 4 This is a schematic diagram of the internal structure of the freeze tube in an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of this disclosure; Figure 5 This is a schematic diagram of the sieving component structure in a freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to an embodiment of this disclosure; Figure 6 This is a schematic diagram of the internal structure of the receiving cylinder and bottom frame in an embodiment of an immune cell protein polypeptide freeze-drying pulverization device. Figure 7 This is a schematic diagram of the connection structure between the insert and the first circular plate and the arc plate in an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of this disclosure; Figure 8 This is a schematic diagram of the connection between the electric push rod and the stirring rack in an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of this disclosure; Figure 9 This is a schematic diagram of the separation of the upper and lower filter frames in an immune cell protein polypeptide freeze-drying pulverization device according to an embodiment of this disclosure; As shown in the figure: 1. Mounting bracket; 2. Piping assembly; 21. Refrigeration pipe; 22. Connecting ring; 23. Inlet pipe; 24. Top frame; 25. Refrigerant pipe; 26. Baffle; 27. Insert rod; 28. Return spring; 29. ​​First gear ring; 210. First gear; 211. First motor; 3. Sieving assembly; 31. Base frame; 32. Collection cylinder; 33. First discharge channel; 34. Expansion layer; 35. Connecting frame; 36. Second motor; 37. Second gear; 38. Second gear ring; 39. Upper filter frame; 310. First screw; 311. Outlet; 312. First circular plate; 313. Insert frame; 314. Second discharge channel; 315. Sealing plate; 316. Lower filter frame; 317. Stirring frame; 318. Third motor; 319. Slide groove; 320. Second circular plate; 321. Arc plate; 322. Sliding block; 323. Electric push rod; 324. Turntable; 325. Side groove; 4. Drive assembly; 41. Fourth motor; 42. Second screw; 43. Moving frame; 44. Ring rope; 45. Limiting frame; 46. Moving plate; 47. Crushing cone. Detailed Implementation

[0019] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0020] like Figure 1, Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 and Figure 8 As shown in the present disclosure, an embodiment of the present disclosure proposes a freeze-drying and pulverizing device for immune cell protein peptides, comprising: a mounting frame 1, a pipe assembly 2 on the top of the mounting frame 1, sieving components 3 mounted at both ends of the mounting frame 1 on the pipe assembly 2, the sieving components 3 communicating with both ends of the pipe assembly 2, and a driving component 4 disposed inside the pipe assembly 2; the pipe assembly 2 includes freezing tubes 25, two sets of freezing tubes 25, top frames 24 rotatably mounted at both ends of the two sets of freezing tubes 25, connecting rings 22 rotatably connected to the outer surfaces of the two ends of the freezing tubes 25 near the top frames 24, the connecting rings 22 being fixedly connected to the top frames 24, and an inlet pipe 23 fixed to the top of the connecting rings 22, the inlet pipe 23 communicating with the inside of the freezing tubes 25 through the connecting rings 22; the driving component 4 includes a moving plate 46, the moving plate 46 being slidably mounted inside the freezing tubes 25, the freezing... A loop rope 44 is sleeved inside the tube 25, with both ends of the loop rope 44 sliding out from the top frame 24. The moving plate 46 is fixed on the surface of the loop rope 44 located inside the freezing tube 25. The sieving assembly 3 includes a bottom frame 31, which is fixed on the mounting bracket 1. A lower filter frame 316 is rotatably installed inside the bottom frame 31, and an upper filter frame 39 is snapped onto the top of the lower filter frame 316. The upper filter frame 39 and the lower filter frame 316 are combined into a hollow cylinder, and filter holes are equidistantly opened on the surfaces of the upper filter frame 39 and the lower filter frame 316. In this scheme, the preparation stage of immune cell protein peptides mainly involves placing the newly prepared immune cell protein peptide solution in the freezing tube 25 and pre-cooling it to -5 to -8°C, with the solution volume not exceeding 75%. The pre-cooled immune cell protein peptides are then crushed, and the freezing tube 25 is rotated to stir and mix. The temperature is set to -32 to -35°C, and the mixture is frozen for 2 to 2.5 seconds. h; Adjust the temperature to -40 to -70°C, freeze for 30 minutes, then evacuate the freezing tank to 10 to 20 Pa; Set a vacuum-heating cycle, that is, when the tank pressure reaches 10 to 20 Pa, stop vacuuming, slowly heat up to allow the tank pressure to rise, and when the tank pressure rises to 90 to 100 Pa, evacuate again to 10 to 20 Pa, and then cycle the vacuum-heating operation, while monitoring the tank temperature; When the tank temperature rises to 55 to 58°C, stop the cycle, evacuate again to a tank pressure of 10 to 13 Pa, and dry for 3 to 5 hours; S3.6, When the moisture content reaches 5% to 8%, the freeze-dried immune cells are pulverized at low temperature and fed into the sieving assembly 3, and sieved with a 50 to 80 mesh sieve to obtain immune cell polypeptide powder. This device belongs to the special equipment for biopharmaceutical manufacturing; gene-engineered drugs and vaccine manufacturing.

[0021] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, in some embodiments, the pipe assembly 2 further includes: a baffle 26, a plug rod 27, and a return spring 28. The two ends of the freezing tube 25 are slidably connected to the baffle 26. A plug rod 27 is fixed to one end of the baffle 26 facing the top frame 24, and the plug rod 27 slides through the top frame 24. A return spring 28 is sleeved on the surface of the protruding end of the plug rod 27, and the other end of the return spring 28 is fixedly connected to the top frame 24. Furthermore, a refrigeration tube 21 is sleeved on the outer surface of the two connecting rings 22 of the freezing tube 25, and the refrigeration tube 21 is fixedly connected to the top frame 24. The device is fixed on the mounting bracket 1. A vacuum pump is mounted on the surface of the mounting bracket 1 and is fixedly connected to the bottom of the connecting ring 22. A first toothed ring 29 is fixedly sleeved at one end of the freezing tube 25 and the top frame 24 at the rotatable connection point. A first gear 210 is rotatably mounted on the top frame 24 between the two first toothed rings 29. The first gear 210 meshes with the first toothed rings 29 on both sides. A first motor 211 is fixed on the top frame 24 outside the first gear 210, and the output end of the first motor 211 is fixedly connected to the first gear 210.

[0022] Understandably, the first motor 211 drives the first gear 210 to rotate and mesh with the first gear rings 29 on both sides, causing the two freezing tubes 25 to rotate, improving the temperature balance of the internal solution in a low-temperature environment. The connecting ring 22 does not rotate with the freezing tubes 25, but its interior is hollow, thus allowing the inlet pipe 23 to feed the solution from the top and into the interior of the freezing tubes 25. The freezing tubes 25 can be cooled by the cooling pipes 21 that are sleeved around them. A temperature sensor is installed on the inner wall of the freezing tubes 25 to monitor the internal temperature at all times. The baffles 26 at both ends of the freezing tubes 25 and the connected sieve assembly 3 can be used alternately, and the powder is alternately fed out from both ends each time it moves, improving the processing efficiency.

[0023] like Figure 2 , Figure 3 and Figure 4 As shown, in some embodiments, the drive assembly 4 further includes: a fourth motor 41, a second screw 42, a moving frame 43, and a limiting frame 45. The second screw 42 is rotatably mounted on the top of the two top frames 24. The fourth motor 41 is fixed on the top of the top frame 24 on one side of the second screw 42, and the output end of the fourth motor 41 is fixedly connected to the second screw 42. The moving frame 43 is threaded onto the surface of the second screw 42, and the two sides of the moving frame 43 are fixedly connected to the loop rope 44 on the outside of the freezing tube 25. The limiting frame 45 is fixed on the top frame 24 at the position where the loop rope 44 passes through, and the loop rope 44 slides through the limiting frame 45. A plurality of crushing cones 47 are fixedly fixed in a ring on both sides of the moving plate 46.

[0024] Understandably, the second screw 42 is rotated by the fourth motor 41, and the moving frame 43 is threadedly engaged with the second screw 42 to move the outer side of the two ring ropes 44. As a result, the inner side of the ring ropes 44 moves along the inside of the freezing tube 25, and the moving plate 46 moves along the inside of the freezing tube 25. In the initial state, the moving plate 46 moves to one end of the freezing tube 25, and the immune cell protein polypeptide solution is fed in from the other end inlet pipe 23. After freezing, the raw material inside the freezing tube 25 is crushed by the crushing cone 47 on the opposite side of the moving plate 46. When the moving plate 46 moves to the side of the baffle 26, the baffle 26 can be opened by squeezing, thereby sending the frozen raw material into the interior of the screening assembly 3. The movement of the second screw 42 is reciprocating. After each movement, the mixture is fed in from the other end, thereby reducing the intermediate waiting time and improving production efficiency.

[0025] like Figure 5 , Figure 6 , Figure 7 , Figure 8 and Figure 9 As shown, in some embodiments, the sieving assembly 3 further includes: a receiving cylinder 32, a first discharge channel 33, an expansion layer 34, and a connecting frame 35. The expansion layer 34 is fixed to both ends of the bottom frame 31 and communicates with the interior of the bottom frame 31. The first discharge channel 33 is fixed to the bottom of the expansion layer 34. The connecting frame 35 is fixed to the top of the bottom frame 31 and is fixedly connected to the bottom of the top frame 24. The receiving cylinder 32 is fixed to the top of the expansion layer 34 on one side of the connecting frame 35 and the bottom frame 31. The upper filter frame 39 slides into the receiving cylinder 32, opening the lower filter frame 316 to receive powder. The connecting frame 35 is located outside the receiving cylinder 32 and is rotatably mounted with a second gear ring 38. The lower filter frame 316 is fixedly connected to the second gear ring 38. A second gear 37 is meshed with one side of the bottom frame 31, and a second motor 36 is fixed to the top of the bottom frame 31 corresponding to the second gear 37. The output end of the second motor 36 is fixedly connected to the second gear 37. The bottom of the bottom frame 316 has side grooves 325 on both sides. The bottom of the bottom frame 39 slides along the inside of the side grooves 325. A first circular plate 312 is fixed to one end of the bottom frame 39 facing the storage cylinder 32. The first circular plate 312 slides along the inside of the storage cylinder 32. A collar is provided on the outer edge of the first circular plate 312. A first screw 310 is rotatably installed on the top of the storage cylinder 32. The first circular plate 312 is threaded onto the surface of the first screw 310 through the collar. A motor that drives the first screw 310 to rotate is fixed to the outer end of the storage cylinder 32.

[0026] It should be noted that the bottom of the first discharge channel 33 is connected to a sealed delivery device, such as a sealed conveyor belt or collection box, to collect the powder. The first screw 310 is driven by a motor to rotate, causing the first circular plate 312 to move the upper filter frame 39 along the top of the lower filter frame 316. The upper filter frame 39 and the lower filter frame 316 slide along the side groove 325. After the upper filter frame 39 is moved away, the frozen material inside the freezing tube 25 is sent into the lower filter frame 316 through the drive assembly 4. The first circular plate 312 is connected to the first screw 310 and the insert 313 through the collar of the outer ring. When the upper filter frame 39 and the lower filter frame 316 rotate, they will not be interfered with by the first screw 310 and the insert 313. After the upper filter frame 39 and the lower filter frame 316 are combined, the second motor 36 drives the second gear 37 to mesh with the second gear ring 38, causing the upper filter frame 39 and the lower filter frame 316 to rotate, thus accelerating the separation and screening of raw materials.

[0027] like Figure 6 , Figure 7 , Figure 8 and Figure 9 As shown, in some embodiments, an arc plate 321 is slidably connected inside the bottom frame 31. A bracket 313 is fixed to the bottom of the collar of the arc plate 321 and the first circular plate 312. The top and bottom of the bracket 313 slide into the storage cylinder 32 and the expansion layer 34, respectively. The storage cylinder 32 has a delivery outlet 311 at the bottom of the expansion layer 34, and a second discharge channel 314 is fixed to the bottom of the delivery outlet 311. Slider blocks 322 are fixed to the top of both ends of the arc plate 321. Slide grooves 319 are formed on both sides of the top of the bottom frame 31 and the expansion layer 34, and the sliders 322 slide along the inside of the slide grooves 319. The outlet end of the second discharge channel 314 extends out of the expansion layer 34, and the second discharge channel 31... A sealing plate 315 is rotatably installed at the through end of 4. A stirring frame 317 is rotatably installed at the axis of the first circular plate 312 at the bottom of the upper filter frame 39. A third motor 318 is fixed at the other end of the first circular plate 312, and the output end of the third motor 318 is fixedly connected to the stirring frame 317. A second circular plate 320 is inserted into the other end of the stirring frame 317. The second circular plate 320 slides along the inside of the lower filter frame 316. A turntable 324 is rotatably installed on the surface of the second circular plate 320 at the insertion end of the stirring frame 317. Electric push rods 323 are fixed on both sides of the turntable 324. An insertion post is fixed at the extended end of the electric push rod 323. Insertion holes are opened on the shaft of the stirring frame 317 corresponding to the positions of the insertion posts on both sides.

[0028] It should be noted that the second circular plate 320 can be inserted into the insertion hole of the stirring frame 317 via the insertion post of the electric push rod 323. Thus, the second circular plate 320 can move along the interior of the lower filter frame 316 along with the stirring frame 317 and the first circular plate 312, pushing unscreened large particles towards the receiving cylinder 32. These particles are then collected through the delivery outlet 311 and the second discharge channel 314. When the first circular plate 312 is drawn into the receiving cylinder 32 as the first screw 310 rotates, the insertion frame 313 drives the arc plate 321 inside the bottom frame 31 to move, sending the fine powder screened by the lower filter frame 316 and the upper filter frame 39 out through the first discharge channel 33 on one side of the receiving cylinder 32. When the first circular plate 312 resets... The arc plate 321 can move from one end of the bottom frame 31 to the other end, sending the powder out from the first discharge channel 33 on the other side. At this time, after the upper filter frame 39 and the lower filter frame 316 screen the raw materials together, the powder is sent out by the movement of the second circular plate 320. When the raw materials enter the lower filter frame 316, the insertion post of the electric push rod 323 separates from the insertion post of the stirring frame 317. The second circular plate 320 remains stationary on the other side. The upper filter frame 39 and the first circular plate 312 move separately into the receiving frame. The stirring frame 317 is driven to rotate by the third motor 318. After the raw materials enter the upper filter frame 39 and the lower filter frame 316, the stirring frame 317 is used to stir the raw materials, thereby speeding up the screening of the raw materials.

[0029] Working principle: When using the apparatus, pre-cool the freshly prepared immune cell protein peptide solution to -5 to -8°C in cryotube 25, ensuring the solution volume does not exceed 75%. Break the pre-cooled immune cell protein peptides, rotate cryotube 25 to mix thoroughly, and set the temperature to -32 to -35°C for 2 to 2.5 hours. Adjust the temperature to -40 to -70°C and freeze for 30 minutes. After n, the freezing tank is evacuated to 10-20 Pa; a vacuum-heating cycle is set, that is, when the tank pressure reaches 10-20 Pa, the vacuuming is stopped, and the tank pressure is slowly increased to restore the pressure. When the tank pressure reaches 90-100 Pa, the vacuuming is evacuated again to 10-20 Pa, and then the vacuum-heating operation is repeated, while monitoring the tank temperature; when the tank temperature reaches 55-58°C, the cycle is stopped, and the tank pressure is evacuated again to 10-13 Pa, and dried for 3-5 hours; S3.6, when the moisture content reaches 5%-8%, the freeze-dried immune cells are subjected to low-temperature pulverization and fed into the sieving assembly 3. The second screw 42 is rotated by the fourth motor 41, and the moving frame 43 is moved. The screw 42, threadedly engaged with the second screw, drives the outer sides of the two ring ropes 44 to move, thereby causing the inner sides of the ring ropes 44 to move along the interior of the freezing tube 25. The moving plate 46 moves along the interior of the freezing tube 25. Initially, the moving plate 46 moves to one end of the freezing tube 25, and the immune cell protein polypeptide solution is fed in from the other end inlet pipe 23. After freezing, the raw material inside the freezing tube 25 is crushed by the crushing cone 47 on the opposite side of the moving plate 46. When the moving plate 46 moves to the side of the baffle 26, the baffle 26 can be opened by squeezing, thereby sending the frozen raw material into the sieve assembly 3. The movement of the second screw 42 is reciprocating. After each movement... The mixture is fed in from the other end, thereby reducing intermediate waiting time and improving production efficiency. It is sieved through a 50-80 mesh sieve to obtain immune cell polypeptides. The bottom of the first discharge channel 33 is connected to a sealed delivery device, such as a sealed conveyor belt or collection box, to collect the powder. The first screw 310 is driven by a motor to rotate, causing the first circular plate 312 to move the upper filter frame 39 along the top of the lower filter frame 316. The upper filter frame 39 and the lower filter frame 316 slide along the side groove 325. After the upper filter frame 39 is removed, the frozen material inside the freezing tube 25 is fed into the lower filter frame 316 through the drive assembly 4. The material is then transported through the collar of the outer ring of the first circular plate 312. Connected to the first screw 310 and the insert 313, the upper filter frame 39 and the lower filter frame 316 rotate without interference from the first screw 310 and the insert 313. After the upper filter frame 39 and the lower filter frame 316 merge, the second motor 36 drives the second gear 37 to mesh with the second gear ring 38, causing the upper filter frame 39 and the lower filter frame 316 to rotate, thus accelerating the separation and screening of raw materials. The second circular plate 320 can be inserted into the insertion hole of the stirring frame 317 through the insertion post of the electric push rod 323. Thus, the second circular plate 320 can move along the interior of the lower filter frame 316 with the stirring frame 317 and the first circular plate 312, pushing the large unscreened particles towards the receiving cylinder 32.The powder is collected and discharged through the outlet 311 and the second discharge channel 314. When the first circular plate 312 is rotated with the first screw 310 and retracts into the receiving cylinder 32, the insert 313 drives the arc plate 321 inside the bottom frame 31 to move, sending the fine powder screened by the lower filter frame 316 and the upper filter frame 39 out through the first discharge channel 33 on one side of the receiving cylinder 32. When the first circular plate 312 is reset, the arc plate 321 can move from one end of the bottom frame 31 to the other end, sending the powder out through the first discharge channel 33 on the other side. At this time, the upper filter frame 39 and the lower filter frame 316 are discharged through the first discharge channel 33 on the other side. After the frames 316 are merged and the raw materials are screened, the powder is conveyed out by the movement of the second circular plate 320. When the receiving raw material enters the lower filter frame 316, the insertion post of the electric push rod 323 separates from the insertion post of the agitator 317. The second circular plate 320 remains stationary on the other side. The upper filter frame 39 and the first circular plate 312 move separately into the receiving frame. The agitator 317 is driven to rotate by the third motor 318. After the raw material enters the upper filter frame 39 and the lower filter frame 316, the agitator 317 agitates the raw material, thereby accelerating the screening process.

[0030] The foregoing has shown and described the basic principles and main features of the present invention and its advantages. It will be apparent to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention.

[0031] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A freeze-drying and pulverizing device for immune cell protein polypeptides, characterized in that, include: Mounting frame (1), the top of the mounting frame (1) is provided with a pipe assembly (2), the mounting frame (1) is provided with a screening assembly (3) at both ends of the pipe assembly (2), the screening assembly (3) is connected to both ends of the pipe assembly (2), and a driving assembly (4) is provided inside the pipe assembly (2). The pipe assembly (2) includes a freezing pipe (25), which is provided in two sets. A top frame (24) is rotatably installed at both ends of the two sets of freezing pipes (25). A connecting ring (22) is rotatably connected to the outer surface of both ends of the freezing pipe (25) near the top frame (24). The connecting ring (22) is fixedly connected to the top frame (24), and an inlet pipe (23) is fixed at the top of the connecting ring (22). The inlet pipe (23) is connected to the inside of the freezing pipe (25) through the connecting ring (22). The drive assembly (4) includes a movable plate (46), which is slidably installed inside the freezing tube (25). A loop rope (44) is sleeved inside the freezing tube (25), and the two ends of the loop rope (44) slide out from the top frame (24). The movable plate (46) is fixed on the surface of the loop rope (44) located inside the freezing tube (25). The sieving assembly (3) includes a bottom frame (31), which is fixed on the mounting frame (1). A lower filter frame (316) is rotatably installed inside the bottom frame (31). An upper filter frame (39) is snapped onto the top of the lower filter frame (316). The upper filter frame (39) and the lower filter frame (316) are combined into a hollow cylinder, and filter holes are equidistantly opened on the surfaces of the upper filter frame (39) and the lower filter frame (316).

2. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 1, characterized in that, The pipe assembly (2) also includes: The two ends of the freezing tube (25) are slidably connected to the baffle (26), the end of the baffle (26) facing the top frame (24) is fixed with the rod (27), and the rod (27) slides out of the top frame (24). The surface of the end of the rod (27) is sleeved with the return spring (28), and the other end of the return spring (28) is fixedly connected to the top frame (24). The refrigeration tube (25) is located on the outer surface of the two connecting rings (22) and is fitted with a refrigeration tube (21). The refrigeration tube (21) is fixed on the mounting bracket (1). A vacuum pump is installed on the surface of the mounting bracket (1) and is fixedly connected to the bottom of the connecting ring (22).

3. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 2, characterized in that, A first toothed ring (29) is fixedly sleeved at one end of the freezing tube (25) and the top frame (24) at the rotatable connection. A first gear (210) is rotatably installed between the two first toothed rings (29) on the top frame (24). The first gear (210) meshes with the first toothed rings (29) on both sides. The top frame (24) is located outside the first gear (210) and a first motor (211) is fixed thereon, and the output end of the first motor (211) is fixedly connected to the first gear (210).

4. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 3, characterized in that, The driving component (4) also includes: The fourth motor (41), the second screw (42), the moving frame (43), and the limiting frame (45) are rotatably mounted on the top of the two top frames (24). The fourth motor (41) is fixed on the top of the top frame (24) on one side of the second screw (42), and the output end of the fourth motor (41) is fixedly connected to the second screw (42). The moving frame (43) is threaded on the surface of the second screw (42), and the two sides of the moving frame (43) are fixedly connected to the loop rope (44) on the outside of the freezing tube (25). The limiting frame (45) is fixed at the position where the loop rope (44) passes through the top frame (24), and the loop rope (44) slides through the limiting frame (45). The movable plate (46) has multiple crushing cones (47) fixed in a ring on both sides.

5. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 4, characterized in that, The sieving assembly (3) further includes: The bottom frame (31) has an expansion layer (34) and a connecting frame (35). The two ends of the bottom frame (31) are fixed with expansion layers (34), and the expansion layers (34) are connected to the inside of the bottom frame (31). The bottom of the expansion layer (34) is fixed with the first discharge channel (33). The top of the bottom frame (31) is fixed with a connecting frame (35), and the connecting frame (35) is fixedly connected to the bottom of the top frame (24). The top of the expansion layer (34) on one side of the connecting frame (35) and the bottom frame (31) is fixed with a storage tube (32). The upper filter frame (39) slides into the inside of the receiving cylinder (32) to open the lower filter frame (316) to receive powder.

6. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 5, characterized in that, The connecting frame (35) is rotatably mounted on the outside of the storage cylinder (32) with a second gear ring (38). The lower filter frame (316) is fixedly connected to the second gear ring (38). The second gear (37) is meshed on one side of the second gear (37). The bottom frame (31) is fixed with a second motor (36) corresponding to the top of the second gear (37), and the output end of the second motor (36) is fixedly connected to the second gear (37). The lower filter frame (316) has side grooves (325) on the top of both sides, and the bottom of the upper filter frame (39) slides along the inside of the side grooves (325).

7. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 6, characterized in that, The upper filter frame (39) is fixed with a first circular plate (312) at one end facing the storage cylinder (32). The first circular plate (312) slides inside the storage cylinder (32). A collar is provided on the outer edge of the first circular plate (312). A first screw (310) is rotatably installed on the top of the storage cylinder (32). The first circular plate (312) is threaded onto the surface of the first screw (310) through the collar. The outer end of the storage tube (32) is fixed with a motor that drives the first screw (310) to rotate.

8. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 7, characterized in that, An arc plate (321) is slidably connected inside the bottom frame (31). A bracket (313) is fixed at the bottom of the collar of the arc plate (321) and the first circular plate (312). The top and bottom of the bracket (313) are slidably inserted into the storage tube (32) and the expansion layer (34) respectively. The storage tube (32) is located at the bottom of the expansion layer (34) and has an outlet (311). A second discharge channel (314) is fixed at the bottom of the outlet (311). A slider (322) is fixed at the top of both ends of the arc plate (321). A groove (319) is opened on both sides of the top of the bottom frame (31) and the expansion layer (34). The slider (322) slides along the inside of the groove (319). The outlet end of the second discharge channel (314) extends through the expansion layer (34), and a sealing plate (315) is rotatably installed at the outlet end of the second discharge channel (314).

9. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 8, characterized in that, The first circular plate (312) is rotatably mounted with a stirring frame (317) at the bottom axis of the upper filter frame (39). The other end of the first circular plate (312) is fixed with a third motor (318), and the output end of the third motor (318) is fixedly connected to the stirring frame (317). The other end of the stirring frame (317) is inserted with a second circular plate (320), and the second circular plate (320) slides along the inside of the lower filter frame (316).

10. The freeze-drying and pulverizing apparatus for immune cell protein polypeptides according to claim 9, characterized in that: The second circular plate (320) is rotatably mounted on the surface of the insertion end of the stirring frame (317), and electric push rods (323) are fixed on both sides of the turntable (324). The extended end of the electric push rod (323) is fixed with a plug, and the shaft of the stirring frame (317) is provided with a plug hole corresponding to the position of the plug on both sides.

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