Microscope objective and optical system with waveband division achromatism and common focus

Abstract

The application provides a waveband-dividing achromatic and confocal microscopic objective and optical system, and the waveband-dividing achromatic and confocal microscopic objective comprises, in sequence from outgoing parallel light to a surface of an observed object, a coaxial double-concave lens A, a double-convex lens B, a double-convex lens C, a double-concave lens D, a double-convex lens E, a double-convex lens F, a double-concave lens G, a meniscus negative lens H, a double-convex lens I, a meniscus positive lens J and a meniscus positive lens K; the double-convex lens C and the double-concave lens D are glued together to form a glued lens CD, the double-convex lens F and the double-concave lens G are glued together to form a glued lens FG, and the meniscus negative lens H and the double-convex lens I are glued together to form a glued lens HI. Compared with the prior art, the waveband-dividing achromatic and confocal microscopic objective has the characteristics of multi-waveband confocal point, complex achromatism, high resolution, super-long working distance and large field of view.

Description

Technical Field

[0001] This invention relates to the field of optical lenses, and in particular to a wavelength-division achromatic and confocal microscope objective and optical system. Background Technology

[0002] In the field of microscopic imaging technology, especially in cutting-edge life science research such as optogenetics, two-photon fluorescence microscopy, and optical manipulation, increasingly stringent requirements are being placed on the multifunctional capabilities of microscope objectives. Researchers not only need to perform high-resolution, wide-field-of-view diffraction-limited imaging of living samples using visible light, but also need to simultaneously utilize near-infrared lasers to precisely stimulate, operate on, or release light onto specific cells or subcellular structures on the same focal plane. On the one hand, currently available commercial objectives cannot achieve strict confocalization in the visible and near-infrared bands. On the other hand, high-magnification, large-aperture microscope objectives generally have very short working distances, making them prone to interference between the objective and coverslip during observation, potentially damaging samples and equipment. Summary of the Invention

[0003] In view of the above-mentioned problems, this invention proposes a wavelength-division achromatic and confocal microscope objective and optical system that can simultaneously image clearly in visible light and near-infrared light in the 780-850nm range. It is achromatic in both wavelength bands, and further achieves apochromatic in the visible light band. The working distance is the same in both wavelength bands, and it has the characteristics of large field of view, ultra-long working distance and high resolution.

[0004] This invention is achieved through the following technical solution:

[0005] This invention first proposes a band-segmented achromatic and confocal microscope objective, which, from the exit parallel light to the observed object surface, sequentially includes a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a meniscus negative lens H, a biconvex lens I, a meniscus positive lens J, and a meniscus positive lens K on the same axis; the biconvex lens C and the biconcave lens D are cemented together to form a cemented lens CD, the biconvex lens F and the biconcave lens G are cemented together to form a cemented lens FG, and the meniscus negative lens H and the biconvex lens I are cemented together to form a cemented lens HI.

[0006] Preferably, the microscope objective satisfies the following condition:

[0007] ;

[0008] Wherein, TTL is the total optical length of the microscope objective. The total focal length of the microscope objective is given.

[0009] Preferably, the microscope objective satisfies the following condition:

[0010] ;

[0011] Wherein, WD is the working distance of the microscope objective. The total focal length of the microscope objective is given.

[0012] Preferably, the combined focal length f of the biconcave lens A and the biconvex lens B is... AB and the total focal length of the microscope objectives. satisfy:

[0013] .

[0014] Preferably, the combined focal length f of the cemented lens CD and the biconvex lens E is... CDE And the total optical length (TTL) of the microscope objective satisfies:

[0015] .

[0016] Preferably, the focal length f of the cemented lens FG is... FG And the total optical length (TTL) of the microscope objective satisfies:

[0017] .0.

[0018] Preferably, the focal length f of the cemented lens HI HI and the total focal length of the microscope objectives. satisfy:

[0019] .

[0020] Preferably, the combined focal length f of the meniscus lens J and the meniscus lens K is... JK And the total optical length (TTL) of the microscope objective satisfies:

[0021] .

[0022] Preferably, to balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass and none of them involve calcium fluoride material.

[0023] Preferably, the microscope objective can be used simultaneously for clear imaging in visible light and near-infrared light (780–850 nm), with achromatic distortion in both bands and the same working distance in both bands; the resolution of the microscope objective is: RMS < 0.45 μm in visible light and RMS < 0.63 μm in infrared light; the wavefront error of the microscope objective is: RMS < 0.072 wavelengths in the center field of view and RMS < 0.1 wavelengths in the edge field of view; the magnification of the microscope objective is 40x; the field of view of the microscope objective is ≥ φ25 mm; and the system distortion of the microscope objective is ≤ ±0.5%.

[0024] The present invention further proposes an optical system, including the above-described band-division achromatic and confocal microscope objective, and an aperture S located at the end of the biconcave lens A.

[0025] Preferably, it also includes a cover glass L located at the end of the meniscus lens K.

[0026] The beneficial effects of this invention are as follows:

[0027] Imaging wavelengths: visible light and 780–850 nm;

[0028] Numerical aperture: NA = 0.8;

[0029] Resolution: RMS < 0.45 μm under visible light, RMS < 0.63 μm under infrared light;

[0030] Wavefront error: RMS < 0.072 wavelengths in the center field of view, and RMS < 0.1 wavelengths in the edge field of view;

[0031] Magnification: 40x (with 200mm tube lens);

[0032] Field of view: ≥φ25mm;

[0033] System distortion: ≤±0.5%;

[0034] Cover glass fits: 0.17mm, BK7 material;

[0035] Compared with existing products, the multi-band achromatic and confocal microscope objective of the present invention has the characteristics of multi-band confocal focus, apochromatic, high resolution, ultra-long working distance and wide field of view. Attached Figure Description

[0036] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 This is a schematic diagram of the optical system structure of Embodiment 2 of the present invention;

[0038] Figure 2 This is the RMS diagram of the wavefront error in the visible light band for Embodiment 2 of the present invention;

[0039] Figure 3 This is the RMS diagram of the wavefront error in the infrared band of Embodiment 2 of the present invention;

[0040] Figure 4 This is the color focus shift diagram in the visible light band of Embodiment 2 of the present invention;

[0041] Figure 5 This is a schematic diagram of the optical system structure of Embodiment 3 of the present invention;

[0042] Figure 6 This is the RMS diagram of the wavefront error in the visible light band for Embodiment 3 of the present invention;

[0043] Figure 7 This is the RMS diagram of the wavefront error in the infrared band of Embodiment 3 of the present invention;

[0044] Figure 8 This is the color focus shift diagram in the visible light band of Embodiment 3 of the present invention;

[0045] Figure 9 This is a schematic diagram of the optical system structure of Embodiment 4 of the present invention;

[0046] Figure 10 This is the RMS diagram of the wavefront error in the visible light band for Embodiment 4 of the present invention;

[0047] Figure 11 This is the RMS diagram of the wavefront error in the infrared band of Embodiment 4 of the present invention;

[0048] Figure 12 This is the color focus shift diagram in the visible light band of Embodiment 4 of the present invention.

[0049] The reference numerals in the diagram are: 1-aperture S, 2-biconcave lens A, 3-biconvex lens B, 4-biconvex lens C, 5-biconcave lens D, 6-biconvex lens E, 7-biconvex lens F, 8-biconcave lens G, 9-negative meniscus lens H, 10-biconvex lens I, 11-positive meniscus lens J, 12-positive meniscus lens K, 13-cover glass L. Detailed Implementation

[0050] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0051] Example 1

[0052] This embodiment proposes a band-segmented achromatic and confocal microscope objective, which, from the exit parallel light to the observed object surface, sequentially includes a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a meniscus negative lens H, a biconvex lens I, a meniscus positive lens J, and a meniscus positive lens K on the same axis; the biconvex lens C and the biconcave lens D are cemented together to form a cemented lens CD, the biconvex lens F and the biconcave lens G are cemented together to form a cemented lens FG, and the meniscus negative lens H and the biconvex lens I are cemented together to form a cemented lens HI.

[0053] In this embodiment, the microscope objective satisfies the following condition:

[0054] ;

[0055] Wherein, TTL is the total optical length of the microscope objective. The total focal length of the microscope objective is given.

[0056] In this embodiment, the microscope objective satisfies the following condition:

[0057] ;

[0058] Wherein, WD is the working distance of the microscope objective. The total focal length of the microscope objective is given.

[0059] In this embodiment, the combined focal length f of the biconcave lens A and the biconvex lens B is... AB and the total focal length of the microscope objectives. satisfy:

[0060] .

[0061] In this embodiment, the combined focal length f of the cemented lens CD and the biconvex lens E is... CDE And the total optical length (TTL) of the microscope objective satisfies:

[0062] .

[0063] In this embodiment, the focal length f of the cemented lens FG is...FG And the total optical length (TTL) of the microscope objective satisfies:

[0064] .0.

[0065] In this embodiment, the focal length f of the cemented lens HI HI and the total focal length of the microscope objectives. satisfy:

[0066] .

[0067] In this embodiment, the combined focal length f of the meniscus lens J and the meniscus lens K is... JK And the total optical length (TTL) of the microscope objective satisfies:

[0068] .

[0069] In this embodiment, to balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass, and none of them involve calcium fluoride material.

[0070] In this embodiment, the microscope objective can be used simultaneously for clear imaging in visible light and near-infrared light (780–850 nm), with achromatic distortion in both bands and the same working distance in both bands. The resolution of the microscope objective is: RMS < 0.45 μm in visible light and RMS < 0.63 μm in infrared light. The wavefront error of the microscope objective is: RMS < 0.072 wavelengths in the center field of view and RMS < 0.1 wavelengths in the edge field of view. The magnification of the microscope objective is 40x. The field of view of the microscope objective is ≥ φ25 mm. The system distortion of the microscope objective is ≤ ±0.5%.

[0071] This embodiment also proposes an optical system, including the aforementioned band-splitting achromatic and confocal microscope objective, an aperture S located at the A end of the biconcave lens, and a cover glass L located at the K end of the meniscus lens.

[0072] Example 2

[0073] See Figure 1This embodiment first proposes a band-segmented achromatic and confocal microscope objective, which, from the exit parallel light to the observed object surface, sequentially includes a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a meniscus negative lens H, a biconvex lens I, a meniscus positive lens J, and a meniscus positive lens K on the same axis; the biconvex lens C and the biconcave lens D are cemented together to form a cemented lens CD, the biconvex lens F and the biconcave lens G are cemented together to form a cemented lens FG, and the meniscus negative lens H and the biconvex lens I are cemented together to form a cemented lens HI.

[0074] In this embodiment, the total optical length (TTL) and total focal length of the microscope objective are... The relationship is:

[0075] .

[0076] In this embodiment, the working distance WD and total focal length of the microscope objective are... The relationship is:

[0077] .

[0078] In this embodiment, the combined focal length of the biconcave lens A and the biconvex lens B is... satisfy:

[0079] .

[0080] In this embodiment, the combined focal length f of the cemented lens CD and the biconvex lens E is... CDE satisfy:

[0081] .

[0082] In this embodiment, the focal length of the cemented lens FG is... satisfy:

[0083] .

[0084] In this embodiment, the focal length of the cemented lens HI satisfy:

[0085] .

[0086] In this embodiment, the combined focal length f of the meniscus lens J and the meniscus lens K is... JK satisfy:

[0087] .

[0088] In this embodiment, to balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass, and none of them involve calcium fluoride material.

[0089] This embodiment also proposes an optical system, including the aforementioned band-splitting achromatic and confocal microscope objective, an aperture S located at the A end of the biconcave lens, and a cover glass L located at the K end of the meniscus lens.

[0090] Starting with the incident parallel light, the surfaces of each lens group, arranged sequentially from the image side to the object side, are numbered. The specific structural parameters are shown in Table 1.

[0091] Table 1. Detailed parameters of each component in this embodiment

[0092]

[0093] The microscope objective and optical system of this embodiment can be used with an achromatic tube with a focal length of 200mm.

[0094] The performance parameters of the microscope objectives and optical system based on the detailed parameters listed in Table 1 are as follows:

[0095] Imaging wavelengths: visible light and near-infrared light;

[0096] Numerical aperture: NA = 0.8;

[0097] Resolution: RMS < 0.45 μm under visible light, RMS < 0.63 μm under infrared light;

[0098] Wavefront error: RMS < 0.051 wavelengths in the center field of view and RMS < 0.096 wavelengths in the edge field of view;

[0099] Magnification: 40x (with 200mm tube lens);

[0100] Field of view: ≥φ25mm;

[0101] System distortion: ≤±0.45%;

[0102] Compatible cover glass: 0.17mm, BK7 material.

[0103] Example 3

[0104] See Figure 5This embodiment first proposes a band-segmented achromatic and confocal microscope objective, which, from the exit parallel light to the observed object surface, sequentially includes a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a meniscus negative lens H, a biconvex lens I, a meniscus positive lens J, and a meniscus positive lens K on the same axis; the biconvex lens C and the biconcave lens D are cemented together to form a cemented lens CD, the biconvex lens F and the biconcave lens G are cemented together to form a cemented lens FG, and the meniscus negative lens H and the biconvex lens I are cemented together to form a cemented lens HI.

[0105] In this embodiment, the relationship between the total optical length TTL and the total focal length f' of the microscope objective is as follows:

[0106] .

[0107] In this embodiment, the relationship between the working distance WD and the total focal length f' of the microscope objective is as follows:

[0108] .

[0109] In this embodiment, the combined focal length of the biconcave lens A and the biconvex lens B is... satisfy:

[0110] .

[0111] In this embodiment, the combined focal length f of the cemented lens CD and the biconvex lens E is... CDE satisfy:

[0112] .

[0113] In this embodiment, the focal length of the cemented lens FG is... satisfy:

[0114] .

[0115] In this embodiment, the focal length of the cemented lens HI satisfy:

[0116] .

[0117] In this embodiment, the combined focal length f of the meniscus lens J and the meniscus lens K is... JK satisfy:

[0118] .

[0119] In this embodiment, to balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass, and none of them involve calcium fluoride material.

[0120] This embodiment also proposes an optical system, including the aforementioned band-splitting achromatic and confocal microscope objective, an aperture S located at the A end of the biconcave lens, and a cover glass L located at the K end of the meniscus lens.

[0121] Starting with the incident parallel light, the surfaces of each lens group, arranged sequentially from the image side to the object side, are numbered. The specific structural parameters are shown in Table 2.

[0122] Table 2. Detailed parameters of each component in this embodiment

[0123]

[0124] The microscope objective and optical system of this embodiment can be used with an achromatic tube with a focal length of 200mm.

[0125] The performance parameters of the microscope objectives and optical system based on the detailed parameters listed in Table 2 are as follows:

[0126] Imaging wavelengths: visible light and near-infrared light;

[0127] Numerical aperture: NA = 0.8;

[0128] Resolution: RMS < 0.45 μm under visible light, RMS < 0.63 μm under infrared light;

[0129] Wavefront error: RMS < 0.053 wavelengths in the center field of view, and RMS < 0.090 wavelengths in the edge field of view;

[0130] Magnification: 40x (with 200mm tube lens);

[0131] Field of view: ≥φ25mm;

[0132] System distortion: ≤±0.5%;

[0133] Compatible cover glass: 0.17mm, BK7 material.

[0134] Example 4

[0135] See Figure 9 This embodiment first proposes a band-segmented achromatic and confocal microscope objective, which, from the exit parallel light to the observed object surface, sequentially includes a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a meniscus negative lens H, a biconvex lens I, a meniscus positive lens J, and a meniscus positive lens K on the same axis; the biconvex lens C and the biconcave lens D are cemented together to form a cemented lens CD, the biconvex lens F and the biconcave lens G are cemented together to form a cemented lens FG, and the meniscus negative lens H and the biconvex lens I are cemented together to form a cemented lens HI.

[0136] In this embodiment, the relationship between the total optical length TTL and the total focal length f' of the microscope objective is as follows:

[0137] .

[0138] In this embodiment, the relationship between the working distance WD and the total focal length f' of the microscope objective is as follows:

[0139] .

[0140] In this embodiment, the combined focal length of the biconcave lens A and the biconvex lens B is... satisfy:

[0141] .

[0142] In this embodiment, the combined focal length f of the cemented lens CD and the biconvex lens E is... CDE satisfy:

[0143] .

[0144] In this embodiment, the focal length of the cemented lens FG is... satisfy:

[0145] .

[0146] In this embodiment, the focal length of the cemented lens HI satisfy:

[0147] .

[0148] In this embodiment, the combined focal length f of the meniscus lens J and the meniscus lens K is... JK satisfy:

[0149] .

[0150] In this embodiment, to balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass, and none of them involve calcium fluoride material.

[0151] This embodiment also proposes an optical system, including the aforementioned band-splitting achromatic and confocal microscope objective, an aperture S located at the A end of the biconcave lens, and a cover glass L located at the K end of the meniscus lens.

[0152] Starting with the incident parallel light, the surfaces of each lens group, arranged sequentially from the image side to the object side, are numbered. The specific structural parameters are shown in Table 3.

[0153] Table 3. Detailed parameters of each component in this embodiment

[0154]

[0155] The microscope objective and optical system of this embodiment can be used with an achromatic tube with a focal length of 200mm.

[0156] The performance parameters of the microscope objectives and optical system based on the detailed parameters listed in Table 2 are as follows:

[0157] Imaging wavelengths: visible light and near-infrared light;

[0158] Numerical aperture: NA = 0.8;

[0159] Resolution: RMS < 0.45 μm under visible light, RMS < 0.63 μm under infrared light;

[0160] Wavefront error: RMS < 0.058 wavelengths in the center field of view, and RMS < 0.01 wavelengths in the edge field of view;

[0161] Magnification: 40x (with 200mm tube lens);

[0162] Field of view: ≥φ25mm;

[0163] System distortion: ≤±0.48%;

[0164] Compatible cover glass: 0.17mm, BK7 material.

[0165] The above embodiments are not intended to limit this application in any way. Although preferred embodiments have been disclosed above, they are not intended to limit this application. Any modifications or variations made by those skilled in the art without departing from the scope of the technical solution disclosed above are equivalent to equivalent implementations and fall within the scope of the technical solution. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A band-specific achromatic and confocal microscope objective, characterized in that: From the point of origin of the parallel light to the surface of the observed object, the lenses in sequence include a biconcave lens A, a biconvex lens B, a biconvex lens C, a biconcave lens D, a biconvex lens E, a biconvex lens F, a biconcave lens G, a negative meniscus lens H, a biconvex lens I, a positive meniscus lens J, and a positive meniscus lens K, all on the same axis. Biconvex lens C and biconcave lens D are cemented together to form a cemented lens CD, biconvex lens F and biconcave lens G are cemented together to form a cemented lens FG, and negative meniscus lens H and biconvex lens I are cemented together to form a cemented lens HI.

2. A dual-band confocal microscope objective as described in claim 1, characterized in that: The microscope objective satisfies the following condition: ； ； Wherein, TTL is the total optical length of the microscope objective. WD is the total focal length of the microscope objective and WD is the working distance of the microscope objective.

3. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: The combined focal length f of the biconcave lens A and the biconvex lens B AB and the total focal length of the microscope objectives. satisfy: 。 4. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: The combined focal length f of the cemented lens CD and the biconvex lens E CDE And the total optical length (TTL) of the microscope objective satisfies: 。 5. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: The focal length f of the cemented lens FG FG And the total optical length (TTL) of the microscope objective satisfies: .0。 6. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: The focal length f of the cemented lens HI HI and the total focal length of the microscope objectives. satisfy: 。 7. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: The combined focal length f of the meniscus lens J and the meniscus lens K JK And the total optical length (TTL) of the microscope objective satisfies: 。 8. A band-specific achromatic and confocal microscope objective as described in claim 1, characterized in that: To balance chromatic aberration, the lenses of the biconvex lens E, biconvex lens F, biconvex lens I, and meniscus lens J are made of low-dispersion glass, and none of them involve calcium fluoride material.

9. An optical system, characterized in that: The microscope objective includes a wavelength-division achromatic and confocal microscope as described in any one of claims 1-8, and further includes an aperture stop S located at the end of the biconcave lens A.

10. The optical system as claimed in claim 9, characterized in that: It also includes a cover glass L located at the end of the meniscus lens K.

Images