A method for improving stress resistance of scirpus mariqueter

Abstract

The present application relates to the technical field of plant culture, and particularly relates to a method for improving the stress resistance of Scirpus mariqueter, which comprises the following steps: cultivating seedlings of Scirpus mariqueter by using a nutrient solution, and spraying a paclobutrazol solution; the nutrient solution comprises macroelements, microelements and an iron source, wherein the macroelements comprise nitrogen, phosphorus and potassium in a molar ratio of 13-17:0.5-3:5.0-7.0; the concentration of the paclobutrazol solution is 50-200 mg / L ‑1 The above method can improve the stress resistance of Scirpus mariqueter, especially the ability of resisting seawater flooding, improve the survival rate of Scirpus mariqueter seedlings in seawater immersion environment, and reduce the biomass reduction caused by seawater flooding, and has the advantages of being simple, easy to implement, controllable conditions and remarkable effect, and improving the recovery effect of coastal beach wetland vegetation.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and in particular to a method for improving the stress resistance of *Scirpus triqueter*. Background Technology

[0002] Enhancing the carbon sequestration capacity of terrestrial ecosystems is a crucial component of achieving carbon neutrality. Among the blue carbon ecosystems of China's coastal wetlands, salt marshes have the largest area and annual carbon sequestration volume; however, their carbon sequestration function has significantly declined in recent years, necessitating urgent ecological restoration of damaged coastal salt marshes. *Scirpus triqueter* ( Bolboschoenoplectus mariqueter It is one of the most important coastal salt marsh plants in East China and the only plant that can grow in the low tide zone in subtropical coastal wetlands. Compared with non-coastal salt marsh plants, salt marsh plants such as *Scirpus triqueter* have a certain tolerance to seawater inundation and have been widely used in the ecological restoration of tidal wetlands.

[0003] Although *Scirpus triquetrum* has a certain tolerance to seawater inundation, it grows better under lower seawater inundation intensity and low salinity stress conditions. Furthermore, seedlings used in ecological restoration projects are generally small and have poor stress resistance. Additionally, due to the significant spatial and temporal heterogeneity of water and salt stress conditions in coastal wetlands, extreme water and salt stress caused by typhoons, storm surges, and seawater intrusion frequently occurs. Therefore, improving the stress resistance of *Scirpus triquetrum* seedlings is one of the key technical challenges currently faced in coastal salt marsh vegetation restoration and ecological restoration projects. Summary of the Invention

[0004] This invention provides a method to improve the stress resistance of *Scirpus triqueter*, in order to solve the problem that *Scirpus triqueter* seedlings have poor water flood tolerance when providing seedlings for coastal salt marsh wetland restoration projects.

[0005] In a first aspect, the present invention provides a method for improving the stress resistance of *Scirpus triquetrum*, the method comprising: cultivating *Scirpus triquetrum* seedlings using a nutrient solution and spraying with paclobutrazol solution; The nutrient solution contains macro-elements, micro-elements, and an iron source, wherein the macro-elements include nitrogen, phosphorus, and potassium in a molar ratio of 13~17:0.5~3:5.0~7.0; The concentration of the paclobutrazol solution is 50-200 mg·L. -1 .

[0006] Paclobutrazol is a triazole plant growth regulator whose core function is to inhibit the biosynthesis of gibberellin (GA), thereby regulating plant growth and development. Its existing functions include inhibiting stem elongation, dwarfing plants, promoting tillering / branching, enhancing drought, cold, and lodging resistance, and delaying plant senescence. It is commonly used to prevent lodging in crops such as rice and wheat, or to control flower bud differentiation in fruit trees and flowers, increase the number of flowers, and improve fruit set.

[0007] This invention unexpectedly discovered that, during the cultivation of *Scirpus triquetrum*, by controlling the ratio of nitrogen, phosphorus, and potassium in the growth environment, especially the phosphorus content, and by spraying paclobutrazol solution, its stress resistance can be significantly improved, resulting in higher survival rates and growth capacity of *Scirpus triquetrum* seedlings planted on coastal mudflats, thereby enhancing the restoration of coastal mudflat wetland vegetation.

[0008] Preferably, the concentration of the paclobutrazol solution is 100-200 mg·L⁻¹. -1 More preferably, 150-200 mg·L⁻¹ -1 Preferably, the molar concentrations of nitrogen, phosphorus, and potassium in the above-mentioned nutrient solution are 13-17 mmol·L⁻¹. -1 0.5~3 mmol·L -1 and 5.0~7.0 mmol·L -1 .

[0009] More preferably, the molar concentration of phosphorus in the above-mentioned nutrient solution is 1.0~2.0 mmol·L⁻¹. -1 .

[0010] This invention has found that adjusting the phosphorus content in the nutrient solution can significantly affect the growth capacity of *Scirpus triquetrum* under seawater immersion, and the aforementioned phosphorus concentration can minimize the decrease in *Scirpus triquetrum* biomass caused by seawater immersion.

[0011] Preferably, the nutrient solution comprises the following components: Ca(NO3)2·4H2O 4.75~6.0 mmol·L -1 KNO3 3.0-5.5 mmol·L -1 KH2PO4 0.5~3 mmol·L -1 , MgSO4·7H2O 1.0~3.0 mmol·L -1 EDTA-FeNa 1.0~3.0 mmol·L -1 H3BO3 45~47 μmol·L -1 , CuSO4·5H2O 0.3~0.4 μmol·L -1 , MnSO4·H2O 5.0~7.0 μmol·L -1 , ZnSO4·7H2O 0.70~0.80 μmol·L -1 (NH4)6MO7O 24 ·4H₂O 0.065~0.075 μmol·L -1 .

[0012] Preferably, the paclobutrazol solution is sprayed starting when the seedlings reach a height of 13-15cm, and sprayed continuously for 2-4 days.

[0013] More preferably, during the spraying period, the paclobutrazol solution is sprayed once a day, with each application consisting of 1 to 3 mL of paclobutrazol solution.

[0014] Preferably, after the spraying is completed, the plants are cultivated for another 25 to 35 days until they reach a height of 20 to 25 cm.

[0015] Preferably, the amount of nutrient solution added is such that the water level on the surface of the ball substrate is maintained at 2-3 cm, and the nutrient solution is replaced with fresh nutrient solution every 5-8 days.

[0016] Preferably, the culture temperature is 20-30℃.

[0017] Preferably, before the seedling cultivation begins, a seed sowing step is also included, wherein the seed sowing includes: after the seeds of *Scirpus triquetrum* have undergone germination treatment, sowing them into the growth substrate when the radicle length does not exceed 0.5 cm; In some embodiments of the present invention, the seed preparation step is further included before the seed germination, specifically including: storing wild-collected *Rubus triquetrum* seeds in water at 0-4℃ for 2 months, soaking them in 0.1% potassium permanganate solution for several minutes and rinsing them clean, germinating them in a petri dish at 25℃ for 3-5 days, and selecting germinated seeds with a radicle length of no more than 0.5 cm for sowing.

[0018] Preferably, the growth substrate comprises quartz sand and vermiculite, with a mass ratio preferably of 1:1 to 3, more preferably 1:2.

[0019] In some embodiments of the present invention, 20-mesh quartz sand and 40-mesh vermiculite are thoroughly mixed as a growth substrate, and the substrate is filled into a culture pot with a height of 13 cm and an inner diameter of 11 cm, with a filling height of 10 cm.

[0020] In some embodiments of the present invention, each of the above-mentioned culture pots is sown with 2 germinated seeds at a depth of 0.5 cm.

[0021] Preferably, the resistance is flood resistance, and more preferably seawater flood resistance.

[0022] The above methods can significantly improve the ability of *Scirpus triqueter* seedlings to resist seawater immersion, especially improving the survival rate and growth capacity of *Scirpus triqueter* seedlings after seawater immersion.

[0023] Secondly, the present invention provides the application of the above-mentioned method for improving the stress resistance of *Scirpus triqueter* in the cultivation of *Scirpus triqueter*.

[0024] Thirdly, the present invention provides a method for cultivating *Scirpus triquetrum*, the method being as described in the first aspect above.

[0025] The present invention has the following beneficial effects: The method for improving the stress resistance of *Scirpus triquetrum* provided by this invention uses the control of nitrogen, phosphorus, and potassium concentrations in the nutrient solution, especially phosphorus concentration, and the application of paclobutrazol to improve the stress resistance of *Scirpus triquetrum*. It has the advantages of being simple and easy to implement, having controllable conditions, and having significant effects. It can greatly improve the survival rate and growth capacity of *Scirpus triquetrum* seedlings in tidal flat habitats and enhance the restoration effect of coastal tidal flat wetland vegetation. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0027] The method for cultivating *Scirpus triqueter* seedlings according to the present invention is as follows: I. Seed Collection: Select healthy and large-area *Scirpus triquetrum* communities in coastal wetlands of East China (Yangtze River Estuary, Hangzhou Bay, Oujiang Estuary, etc.) to determine the seed collection site, and collect seeds from individuals with good growth and large inflorescences.

[0028] II. Substrate preparation: Mix 20-mesh quartz sand and 40-mesh vermiculite thoroughly to form the growth substrate. Fill the substrate into a culture pot with a height of 13cm and an inner diameter of 11cm, with a filling height of 10cm.

[0029] III. Seed sowing and seedling cultivation: Sow the germinated seeds from step one into culture pots, burying them 0.5cm deep, with 2 seeds sown in each culture pot. Use Hoagland nutrient solution, maintain a water level of 2-3cm on the substrate surface, and change the nutrient solution once a week. The cultivation temperature is 20-30℃.

[0030] IV. Spraying plant growth regulators: When the plant height reaches 13-15 cm, spray each seedling with different concentrations of paclobutrazol (Sichuan Runer Technology Co., Ltd., pesticide registration certificate number PD20081543) or purified water (control group), spraying continuously for 3 days, once a day, each time spraying 2 ml of paclobutrazol or purified water per seedling.

[0031] 5. Continue cultivation at a temperature of 20℃-30℃ for 1 month, changing the Hoagland nutrient solution once a week, until the plant height reaches 20-25 cm, at which point the seedling cultivation is complete.

[0032] The specific implementation method is as follows: Example 1: The following methods can be used to improve the stress resistance of *Scirpus triquetrum*: I. Seed preparation: After storing wild-collected seeds of *Rubus triquetrum* in water at 0-4℃ for 2 months, soak them in a 0.1% potassium permanganate solution for a few minutes and rinse them clean. Germinate them in a petri dish at 25℃ for 3-5 days. Select seeds with a radicle length of no more than 0.5 cm for sowing.

[0033] II. Substrate preparation: Mix 20-mesh quartz sand and 40-mesh vermiculite thoroughly to form the growth substrate. Fill the substrate into a culture pot with a height of 13cm and an inner diameter of 11cm, with a filling height of 10cm.

[0034] III. Seed sowing and seedling cultivation: Sow the germinated seeds from step one into culture pots, burying them 0.5cm deep, with 2 seeds sown in each culture pot. Use Hoagland nutrient solution, maintain a water level of 2-3cm on the substrate surface, and change the nutrient solution once a week. The cultivation temperature is 20-30℃.

[0035] IV. Spraying Plant Growth Regulators: When the plant height reaches 13-15 cm, spray each seedling with paclobutrazol solution for 3 consecutive days, once a day, with 2 ml of paclobutrazol solution sprayed each time. The concentrations of paclobutrazol solution were set at 50, 100, 150, and 200 mg / L. -1 The control group was the one sprayed with an equal amount of purified water.

[0036] 5. Continue cultivation at a temperature of 20℃-30℃ for 1 month, changing the Hoagland nutrient solution once a week, until the plant height reaches 20-25 cm, at which point the seedling cultivation is complete. The content of each component in the Hoagland nutrient solution mentioned in step three is: 4.75 mmol·L⁻¹ -1 Ca(NO3)2·4H2O, 5.5 mmol·L -1 KNO3, 0.5 mmol·L -1 KH2PO4, 2.0 mmol·L -1 MgSO4·7H2O, 2.0 mmol·L - 1 EDTA-FeNa, 46.26 μmol·L -1H3BO3, 0.32 μmol·L -1 CuSO4·5H2O, 6.0 μmol·L -1 MnSO4·H2O, 0.77 μmol·L -1 ZnSO4·7H2O, 0.0695 μmol·L -1 (NH4)6MO7O 24 ·4H₂O. The phosphorus content in this experiment was 0.5 mmol·L⁻¹. -1 .

[0037] Example 2: The following methods can be used to improve the stress resistance of *Scirpus triquetrum*: I. Seed preparation: After storing wild-collected seeds of *Rubus triquetrum* in water at 0-4℃ for 2 months, soak them in a 0.1% potassium permanganate solution for a few minutes and rinse them clean. Germinate them in a petri dish at 25℃ for 3-5 days. Select seeds with a radicle length of no more than 0.5 cm for sowing.

[0038] II. Substrate preparation: Mix 20-mesh quartz sand and 40-mesh vermiculite thoroughly to form the growth substrate. Fill the substrate into a culture pot with a height of 13cm and an inner diameter of 11cm, with a filling height of 10cm.

[0039] III. Seed sowing and seedling cultivation: Sow the germinated seeds from step one into culture pots, burying them 0.5cm deep, with 2 seeds sown in each culture pot. Use Hoagland nutrient solution, maintain a water level of 2-3cm on the substrate surface, and change the nutrient solution once a week. The cultivation temperature is 20-30℃.

[0040] IV. Spraying Plant Growth Regulators: When the plant height reaches 13-15 cm, spray each seedling with paclobutrazol solution for 3 consecutive days, once a day, with 2 ml of paclobutrazol solution sprayed each time. The concentrations of paclobutrazol solution were set at 50, 100, 150, and 200 mg / L. -1 The control group was the one sprayed with an equal amount of purified water.

[0041] 5. Continue cultivation at a temperature of 20℃-30℃ for 1 month, changing the Hoagland nutrient solution once a week, until the plant height reaches 20-25 cm, at which point the seedling cultivation is complete. The content of each component in the Hoagland nutrient solution mentioned in step three is: 5.0 mmol·L⁻¹ -1 Ca(NO3)2·4H2O, 5.0 mmol·L -1 KNO3, 1.0 mmol·L -1 KH2PO4, 2.0 mmol·L-1 MgSO4·7H2O, 2.0 mmol·L - 1 EDTA-FeNa, 46.26 μmol·L -1 H3BO3, 0.32 μmol·L -1 CuSO4·5H2O, 6.0 μmol·L -1 MnSO4·H2O, 0.77 μmol·L -1 ZnSO4·7H2O, 0.0695 μmol·L -1 (NH4)6MO7O 24 • 4H₂O. In this example, the phosphorus content is 1.0 mmol·L⁻¹. -1 .

[0042] Example 3: The following methods can be used to improve the stress resistance of *Scirpus triquetrum*: I. Seed preparation: After storing wild-collected seeds of *Rubus triquetrum* in water at 0-4℃ for 2 months, soak them in a 0.1% potassium permanganate solution for a few minutes and rinse them clean. Germinate them in a petri dish at 25℃ for 3-5 days. Select seeds with a radicle length of no more than 0.5 cm for sowing.

[0043] II. Substrate preparation: Mix 20-mesh quartz sand and 40-mesh vermiculite thoroughly to form the growth substrate. Fill the substrate into a culture pot with a height of 13cm and an inner diameter of 11cm, with a filling height of 10cm.

[0044] III. Seed sowing and seedling cultivation: Sow the germinated seeds from step one into culture pots, burying them 0.5cm deep, with 2 seeds sown in each culture pot. Use Hoagland nutrient solution, maintain a water level of 2-3cm on the substrate surface, and change the nutrient solution once a week. The cultivation temperature is 20-30℃.

[0045] IV. Spraying Plant Growth Regulators: When the plant height reaches 13-15 cm, spray each seedling with paclobutrazol solution for 3 consecutive days, once a day, with 2 ml of paclobutrazol solution sprayed each time. The concentrations of paclobutrazol solution were set at 50, 100, 150, and 200 mg / L. -1 The control group was the one sprayed with an equal amount of purified water.

[0046] 5. Continue cultivation at a temperature of 20℃-30℃ for 1 month, changing the Hoagland nutrient solution once a week, until the plant height reaches 20-25 cm, at which point the seedling cultivation is complete. The content of each component in the Hoagland nutrient solution mentioned in step three is 5.5 mmol·L⁻¹.-1 Ca(NO3)2·4H2O, 4.0 mmol·L -1 KNO3, 2.0 mmol·L -1 KH2PO4, 2.0 mmol·L -1 MgSO4·7H2O, 2.0 mmol·L - 1 EDTA-FeNa, 46.26 μmol·L -1 H3BO3, 0.32 μmol·L -1 CuSO4·5H2O, 6.0 μmol·L -1 MnSO4·H2O, 0.77 μmol·L -1 ZnSO4·7H2O, 0.0695 μmol·L -1 (NH4)6MO7O 24 ·4H₂O. In this example, the phosphorus content is 2.0 mmol·L⁻¹. -1 .

[0047] Example 4: The following methods can be used to improve the stress resistance of *Scirpus triquetrum*: I. Seed preparation: After storing wild-collected seeds of *Rubus triquetrum* in water at 0-4℃ for 2 months, soak them in a 0.1% potassium permanganate solution for a few minutes and rinse them clean. Germinate them in a petri dish at 25℃ for 3-5 days. Select seeds with a radicle length of no more than 0.5 cm for sowing.

[0048] II. Substrate preparation: Mix 20-mesh quartz sand and 40-mesh vermiculite thoroughly to form the growth substrate. Fill the substrate into a culture pot with a height of 13cm and an inner diameter of 11cm, with a filling height of 10cm.

[0049] III. Seed sowing and seedling cultivation: Sow the germinated seeds from step one into culture pots, burying them 0.5cm deep, with 2 seeds sown in each culture pot. Use Hoagland nutrient solution, maintain a water level of 2-3cm on the substrate surface, and change the nutrient solution once a week. The cultivation temperature is 20-30℃.

[0050] IV. Spraying Plant Growth Regulators: When the plant height reaches 13-15 cm, spray each seedling with paclobutrazol solution for 3 consecutive days, once a day, with 2 ml of paclobutrazol solution sprayed each time. The concentrations of paclobutrazol solution were set at 50, 100, 150, and 200 mg / L. -1 The control group was the one sprayed with an equal amount of purified water.

[0051] 5. Continue cultivation at a temperature of 20℃-30℃ for one month, changing the Hoagland nutrient solution once a week, until the plant height reaches 20-25 cm, at which point the seedling cultivation is complete.

[0052] The content of each component in the Hoagland nutrient solution mentioned in step three is 6.0 mmol·L⁻¹. -1 Ca(NO3)2·4H2O, 3.0 mmol·L -1 KNO3, 3.0 mmol·L -1 KH2PO4, 2.0 mmol·L -1 MgSO4·7H2O, 2.0 mmol·L - 1 EDTA-FeNa, 46.26 μmol·L -1 H3BO3, 0.32 μmol·L -1 CuSO4·5H2O, 6.0 μmol·L -1 MnSO4·H2O, 0.77 μmol·L -1 ZnSO4·7H2O, 0.0695 μmol·L -1 (NH4)6MO7O 24 ·4H₂O. In this example, the phosphorus content is 3.0 mmol·L⁻¹. -1 .

[0053] The phosphorus content, Ca(NO3)2·4H2O, KNO3 and KH2PO4 concentrations in the Hoagland nutrient solution used in Examples 1-4 above are shown in Table 1.

[0054] Table 1

[0055] Test case Seaweed treated with paclobutrazol at various concentrations in Examples 1-4 were subjected to seawater flooding. The survival rate and percentage decrease in biomass after treatment were measured relative to the control (no flooding treatment). The seawater flooding treatment lasted for 60 days, with a continuous flooding time of 6 hours per day (10:00-16:00), a flooding depth of 35 cm, and a seawater salinity of 30‰.

[0056] The survival rate (%) of *Scirpus triqueter* seedlings in Examples 1-4 is shown in Table 2: Table 2

[0057] Table 3 shows the percentage decrease in biomass of *Scirpus triqueter* seedlings after treatment with different concentrations of paclobutrazol in Examples 1-4 compared to the control (no waterlogging treatment). Table 3

[0058] As shown in Table 2 above, the survival rate of seedlings in the control group without paclobutrazol was only 66.7%–82.1%, and it decreased with decreasing phosphorus concentration in the nutrient solution, indicating that low phosphorus conditions exacerbate the lethal effect of seawater flooding on *Scirpus triqueter* seedlings. After paclobutrazol application, the survival rate of seedlings in all examples significantly increased with increasing paclobutrazol concentration; when the paclobutrazol concentration reached 150 mg·L⁻¹… -1 At these times, the seedling survival rate in all four examples reached 100%, indicating that appropriate concentrations of paclobutrazol can effectively ensure the survival of *Scirpus triqueter* seedlings under extreme seawater flooding conditions. As shown in Table 3 above, the trend of retained biomass was generally consistent with the survival rate, and paclobutrazol treatment significantly reduced biomass loss caused by seawater flooding. Without paclobutrazol application, the biomass reduction rate of seedlings in each example compared to the unflooded control was as high as 72.4%–88.2%; with increasing paclobutrazol concentration, the biomass reduction rate gradually decreased, reaching a minimum at 100 mg·L⁻¹. -1 The percentage decreased to 34.2%–48.3% at 150 mg / L. -1 The concentration further decreased to 19.3%–23.4%, the lowest among all concentrations. It is noteworthy that when the paclobutrazol concentration continued to increase to 200 mg / L... -1 At that time, the rate of biomass decline actually rebounded slightly.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for improving the stress resistance of *Scirpus triqueter*, characterized in that, The method includes: cultivating seedlings of *Scirpus triquetrum* using a nutrient solution and spraying with paclobutrazol solution; The nutrient solution contains macro-elements, micro-elements, and an iron source, wherein the macro-elements include nitrogen, phosphorus, and potassium in a molar ratio of 13~17:0.5~3:5.0~7.0; The concentration of the paclobutrazol solution is 50-200 mg·L -1 .

2. The method for improving the stress resistance of *Scirpus triqueter* according to claim 1, characterized in that, The molar concentrations of nitrogen, phosphorus and potassium in the nutrient solution are 13-17 mmol·L -1 , 0.5-3 mmol·L -1 and 5.0-7.0 mmol·L -1 , respectively.

3. The method for improving the stress resistance of *Scirpus triquetrum* according to claim 1 or 2, characterized in that, The nutrient solution comprises the following components: Ca(NO3)2·4H2O 4.75~6.0 mmol·L -1 KNO3 3.0-5.5 mmol·L -1 KH2PO4 0.5~3 mmol·L -1 , MgSO4·7H2O 1.0~3.0 mmol·L -1 EDTA-FeNa 1.0~3.0 mmol·L -1 H3BO3 45~47 μmol·L -1 , CuSO4·5H2O 0.3~0.4 μmol·L -1 , MnSO4·H2O 5.0~7.0 μmol·L -1 , ZnSO4·7H2O 0.70~0.80 μmol·L -1 (NH4)6MO7O 24 ·4H₂O 0.065~0.075 μmol·L -1 .

4. The method for improving the stress resistance of *Scirpus triquetrum* according to any one of claims 1 to 3, characterized in that, The application of paclobutrazol solution should begin when the seedlings reach a height of 13-15cm and continue for 2-4 days.

5. The method for improving the stress resistance of *Scirpus triqueter* according to claim 4, characterized in that, During the application period, spray once a day, each time applying 1-3 mL of paclobutrazol solution.

6. The method for improving the stress resistance of *Scirpus triquetrum* according to any one of claims 1 to 5, characterized in that, After the spraying is completed, continue cultivation for 25 to 35 days until the plant height reaches 20 to 25 cm; Preferably, the amount of nutrient solution added is such that the water level on the substrate surface is maintained at 2-3 cm, and the nutrient solution is replaced with fresh nutrient solution every 5-8 days.

7. The method for improving the stress resistance of *Scirpus triqueter* according to any one of claims 1 to 6, characterized in that, The culture temperature is 20-30℃.

8. The method for improving the stress resistance of *Scirpus triqueter* according to any one of claims 1 to 7, characterized in that, Before the seedling cultivation begins, a seed sowing step is also included, which includes: after the seeds of *Scirpus triquetrum* have undergone germination treatment, sowing them into the growth substrate when the radicle length does not exceed 0.5 cm; Preferably, the growth substrate comprises quartz sand and vermiculite, with a volume ratio preferably of 1:1 to 3.

9. The method for improving the stress resistance of *Scirpus triqueter* according to any one of claims 1 to 8, characterized in that, The aforementioned resistance is flood resistance, preferably seawater flood resistance.

10. The application of the method for improving the stress resistance of *Scirpus triquetrum* according to any one of claims 1 to 9 in the cultivation of *Scirpus triquetrum*.

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