Cloud tobacco extract and method of making same

Abstract

The application discloses Yunyan extract and a preparation method thereof, and belongs to the technical field of tobacco processing. The preparation method comprises the following steps: mixing middle tobacco leaves, dalaoye flowers and honeysuckle flowers, and then carrying out pre-wetting treatment; subsequently, carrying out two-stage water extraction by adopting a gradient extraction process; after centrifugation, carrying out complex enzymolysis treatment by adopting pectinase and cellulase; and then, after clarification filtration and reduced-pressure concentration, adding tea polyphenols to carry out stabilization treatment, so as to obtain the Yunyan extract. The application can effectively improve the content of aroma components such as linalool, nerol, phenethyl alcohol and geraniol, improve the extract yield and sensory quality, and improve the storage stability of the product by means of raw material synergistic compounding, gradient extraction and complex enzymolysis process and tea polyphenol stabilization treatment. The obtained Yunyan extract has the advantages of prominent floral fragrance, strong sweet feeling, good aroma coordination and low irritation, and has a good industrial application prospect.

Description

Technical Field

[0001] This invention belongs to the field of tobacco processing technology, and more specifically, relates to a Yunnan tobacco extract and its preparation method. Background Technology

[0002] Tobacco extract is a paste or semi-paste extract obtained by solvent extraction and concentration of tobacco or plant raw materials. As an important natural flavoring component, it is widely used in cigarettes, cigars, and new tobacco products. High-quality tobacco extract can impart a full and rounded aroma to tobacco products, reduce off-flavors and irritation, and enhance the overall sensory quality.

[0003] Yunnan tobacco leaves, due to their unique geographical environment and climate, are characterized by a prominent sweet aroma, delicate fragrance, and moderate nicotine content, making them a superior raw material for preparing high-quality tobacco extracts. Among them, the re-dried fragments of the middle part of the tobacco leaves, produced during the re-drying process, contain abundant aroma components and are often used as raw materials for extracting extracts. However, extracts prepared solely from Yunnan tobacco leaves or their re-dried fragments have a relatively limited aroma profile, making it difficult to meet diverse flavoring needs in practical applications.

[0004] Bitter orange blossoms are the dried flower buds of the bitter orange tree (Bitter Orange), a plant in the Rutaceae family, and have a unique floral and fruity fragrance. Honeysuckle is the dried flower bud or newly opened flower of the honeysuckle plant (Lonicera japonica), a plant in the Caprifoliaceae family, and has a sweet floral fragrance with a fresh and elegant aroma. Both have wide applications in the field of natural fragrances.

[0005] However, there are currently no reports on the preparation of Yunnan tobacco extract by compounding and extracting fragments of re-dried tobacco leaves from central Yunnan with bitter orange flowers and honeysuckle. There is also a lack of scientific optimization methods for the compounding ratio of the above raw materials, making it difficult to obtain Yunnan tobacco extract products with stable aroma quality and distinctive style. Summary of the Invention

[0006] In view of the above-mentioned problems in the existing technology, the technical problem to be solved by the present invention is to provide a method for preparing tobacco extract.

[0007] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0008] A method for preparing tobacco extract includes the following steps:

[0009] 1) After mixing the re-drying fragments of tobacco leaves, bitter orange blossoms and honeysuckle, pre-moisten them to obtain the mixed raw materials;

[0010] 2) Add pure water to the mixed raw materials obtained in step 1) and perform gradient temperature extraction, and perform a second extraction. Combine the extracts to obtain a composite extract.

[0011] 3) Add pectinase and cellulase to the compound extract obtained in step 2) for enzymatic hydrolysis, and then inactivate the enzymes;

[0012] 4) Centrifuge and filter the extract obtained after enzymatic hydrolysis in step 3) to obtain a clear extract;

[0013] 5) After the clarified extract obtained in step 4) is concentrated under reduced pressure, tea polyphenols are added for stabilization treatment, and nitrogen is used for packaging to obtain Yunnan tobacco extract.

[0014] Preferably, in step 1), the mass ratio of the re-drying fragments of the middle tobacco leaves, the bitter orange blossoms, and the honeysuckle is 58~64:20~26:16.

[0015] Preferably, in step 1), the bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments in the middle is controlled to be 10%~14%.

[0016] Preferably, in step 1), the pre-wetting treatment is as follows: spray 8-12 g of pure water onto 100 g of mixed raw materials and let it stand at 20-30°C for 20-30 min.

[0017] Preferably, in step 2), the gradient temperature extraction process is as follows: the first stage extraction temperature is 50~54℃ and the extraction time is 28~30 min; the second stage extraction temperature is 58~61℃ and the extraction time is 42~45 min; then the filter residue is extracted a second time at an extraction temperature of 58~61℃ for 35 min.

[0018] Preferably, in step 2), the pH of the system is controlled at 5.8 to 6.2 during the gradient extraction process.

[0019] Preferably, in step 3), the enzymatic hydrolysis treatment involves: pectinase addition of 0.4~0.55 g; cellulase addition of 0.22~0.25 g; hydrolysis temperature of 46~48℃; and hydrolysis time of 35~42 min.

[0020] Preferably, in step 5), the vacuum concentration conditions are: vacuum degree of -0.09 to -0.088 MPa; water bath temperature of 52 to 55°C; and concentration to a relative density of 1.17 to 1.19.

[0021] Preferably, in step 5), the amount of tea polyphenols added is 0.1% to 0.15% of the mass of Yunnan tobacco extract, and the mixture is stirred at 40°C for 10 to 20 minutes.

[0022] The cloud tobacco extract prepared by the aforementioned method.

[0023] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0024] 1) This invention uses re-dried tobacco leaf fragments, bitter orange blossoms and honeysuckle as compound raw materials. Through the synergistic combination of the three, the floral aroma and sweetness of Yunnan tobacco extract can be effectively enhanced, so that the resulting extract has the characteristics of delicate aroma, good harmony and comfortable aftertaste.

[0025] 2) This invention employs a gradient extraction process, which improves the release efficiency of aroma-producing components such as linalool, nerol, phenylethyl alcohol, and geraniol in the raw materials by extracting them through staged heating, thereby increasing the content of total volatile aroma components.

[0026] 3) This invention uses a combination of pectinase and cellulase for enzymatic hydrolysis, which can effectively destroy the plant cell wall structure, promote the release of aroma precursors, increase the yield of extract and the fullness of aroma, and reduce off-flavors and irritation.

[0027] 4) By controlling the reduced pressure concentration temperature and vacuum conditions, this invention can effectively reduce the loss of heat-sensitive volatile components and improve the retention rate of floral aroma components in tobacco extract.

[0028] 5) This invention employs tea polyphenol stabilization treatment combined with nitrogen replacement packaging, which can effectively improve the storage stability of Yunnan tobacco extract, slow down the oxidation loss of volatile aroma components, extend the product shelf life, and has good industrial application value. Attached Figure Description

[0029] Figure 1 This is a process flow diagram for preparing tobacco extract according to the present invention;

[0030] Figure 2 The sensory evaluation radar charts for Example 1 and Comparative Examples 1, 4, and 6 are shown. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of this invention clearer, the invention is further described below with reference to specific embodiments. Unless otherwise specified, the technical means used in the following embodiments are all conventional means well known to those skilled in the art. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0032] This application provides a method for preparing tobacco extract, such as... Figure 1 As shown, it includes the following steps:

[0033] 1) Raw material pretreatment: Yunnan-produced central tobacco leaf re-drying fragments are mixed with bitter orange flowers and honeysuckle in a certain proportion and pre-moistened to allow the plant tissues to absorb water and swell, and increase cell wall permeability, so as to adjust the raw material particle size and moisture content and improve the release efficiency of aroma components.

[0034] 2) Gradient temperature extraction: First, a preliminary extraction is performed at a lower temperature to reduce the loss of low-boiling-point volatile aroma components. Then, the extraction temperature is increased to promote the release of medium- and high-boiling-point aroma components and functional substances. A second extraction is then performed to increase the yield of aroma components, thereby maintaining the floral and sweet aroma characteristics and improving the aroma layering and harmony.

[0035] 3) Compound enzymatic hydrolysis: Pectinase and cellulase are added to the extract to moderately degrade the plant cell wall, promote the release of bound aroma precursors, increase the content of volatile aroma components and reduce macromolecular impurities, and improve the sensory quality and clarity of the extract.

[0036] 4) Centrifugal filtration: Removes insoluble particles and suspended impurities from the extract, improves the uniformity of the extract and the stability of subsequent concentration, and reduces coking and precipitation during the concentration process.

[0037] 5) Low-temperature vacuum concentration: Low-temperature vacuum concentration is used to reduce the loss of heat-sensitive aroma components, increase the concentration of effective aroma components, and make the extract have a full aroma and stability.

[0038] 6) Stabilization treatment: Tea polyphenols are added for antioxidant protection, and nitrogen replacement packaging is used to reduce the oxidation loss of volatile aroma components during storage and improve product stability.

[0039] This application utilizes the synergistic effects of synergistic compounding of floral plants, gradient temperature extraction, and complex enzymatic hydrolysis to increase the content of volatile aroma components in Yunnan tobacco extract, improve the floral characteristics, sweetness, and aroma harmony, and reduce off-flavors and irritation, thereby enhancing the overall sensory quality and storage stability of Yunnan tobacco extract.

[0040] The tobacco leaf fragments used in the following examples were derived from tobacco leaf fragments generated during the re-drying process of Yunnan-produced flue-cured tobacco leaves. The tobacco leaf part grade was middle-grade. The pectinase activity used was 30,000 U / g, and the cellulase activity was 20,000 U / g. The purity of the tea polyphenols used was ≥98%. The pH of the system was adjusted using 0.1 mol / L citric acid solution and 0.1 mol / L sodium hydroxide solution.

[0041] Example 1

[0042] A method for preparing tobacco extract, the specific steps of which are as follows:

[0043] 1) Raw material pretreatment

[0044] Weigh out 60 g of re-dried tobacco leaf fragments, 24 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0045] 2) Gradient Extraction

[0046] Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6; first, extract at 52℃ and 240 r / min for 28 min with stirring; then raise the temperature to 60℃ and continue stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract at 60℃ for 35 min with stirring, filter and collect the second extract, and combine the two extracts to obtain a composite extract;

[0047] 3) Compound enzymatic hydrolysis treatment

[0048] After centrifuging the compound extract at 5000 r / min for 8 min, the supernatant was taken and cooled to 48℃. Then, 0.5 g of pectinase and 0.22 g of cellulase were added, and the mixture was enzymatically hydrolyzed at 48℃ for 35 min with slow stirring at 100 r / min during the hydrolysis. After the hydrolysis was completed, the mixture was heated at 85℃ for 5 min to inactivate the enzyme.

[0049] 4) Clarification process

[0050] After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, and filter the supernatant through a 0.45 μm microporous membrane to obtain a clear extract.

[0051] 5) Reduced pressure concentration

[0052] The clarified extract was placed in a rotary evaporator and concentrated under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature was controlled at 54℃ and the rotation speed was controlled at 80 r / min. Concentration was stopped when the relative density at 54℃ was 1.17~1.19, and then the extract was rapidly cooled to below 30℃.

[0053] 6) Stabilization treatment

[0054] Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40°C for 15 min, pack with nitrogen purging 3 times, seal, and store at 4°C in the dark to obtain tobacco extract.

[0055] Example 2

[0056] A method for preparing tobacco extract, the specific steps of which are as follows:

[0057] 1) Weigh 62 g of re-dried tobacco leaf fragments, 22 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are crushed to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0058] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6; first, extract at 50℃ and 240 r / min for 30 min by stirring; then raise the temperature to 58℃ and continue to extract by stirring for 45 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again, extract by stirring at 58℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0059] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.5 g of pectinase and 0.22 g of cellulase and hydrolyze at 48℃ for 35 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0060] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0061] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 54℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 54℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0062] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0063] Example 3

[0064] A method for preparing tobacco extract, the specific steps of which are as follows:

[0065] 1) Weigh out 58 g of re-dried tobacco leaf fragments, 26 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are crushed to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0066] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 5.8; first, extract at 52℃ and 240 r / min for 28 min by stirring; then raise the temperature to 60℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 60℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0067] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.45 g of pectinase and 0.22 g of cellulase and hydrolyze at 48℃ for 35 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0068] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0069] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 54℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 54℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0070] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0071] Example 4

[0072] A method for preparing tobacco extract, the specific steps of which are as follows:

[0073] 1) Weigh 63 g of re-dried tobacco leaf fragments, 21 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0074] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6; first, extract at 54℃ and 240 r / min for 28 min by stirring; then raise the temperature to 60℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 60℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0075] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.5 g of pectinase and 0.22 g of cellulase and hydrolyze at 48℃ for 35 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0076] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0077] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 55℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 55℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0078] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0079] Example 5

[0080] A method for preparing tobacco extract, the specific steps of which are as follows:

[0081] 1) Weigh 61 g of re-dried tobacco leaf fragments, 23 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0082] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6.1; first, extract at 52℃ and 240 r / min for 28 min by stirring; then raise the temperature to 59℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 59℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0083] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.55 g of pectinase and 0.25 g of cellulase and hydrolyze at 47℃ for 38 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0084] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0085] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 54℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 54℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0086] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0087] Example 6

[0088] A method for preparing tobacco extract, the specific steps of which are as follows:

[0089] 1) Weigh 60 g of re-dried tobacco leaf fragments, 24 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are crushed to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0090] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6.2; first, extract at 53℃ and 240 r / min for 28 min by stirring; then raise the temperature to 61℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 61℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0091] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.5 g of pectinase and 0.22 g of cellulase and hydrolyze at 48℃ for 35 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0092] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0093] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 52℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 52℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0094] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0095] Example 7

[0096] A method for preparing tobacco extract, the specific steps of which are as follows:

[0097] 1) Weigh 64g of re-dried tobacco leaf fragments, 20g of bitter orange blossoms, and 16g of honeysuckle. The bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100g of mixed raw materials. Then spray 10g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0098] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6; first, extract at 52℃ and 240 r / min for 28 min by stirring; then raise the temperature to 60℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 60℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0099] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.4 g of pectinase and 0.22 g of cellulase and hydrolyze at 46℃ for 42 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0100] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0101] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.088 MPa. During the concentration process, the water bath temperature is controlled at 54℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 54℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0102] 6) Dissolve 0.12 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40℃ for 15 min, pack with nitrogen purging 3 times, seal, and store at 4℃ in the dark to obtain tobacco extract.

[0103] Example 8

[0104] A method for preparing tobacco extract, the specific steps of which are as follows:

[0105] 1) Weigh out 59 g of re-dried tobacco leaf fragments, 25 g of bitter orange blossoms, and 16 g of honeysuckle. The bitter orange blossoms and honeysuckle are crushed to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments is controlled at 12%. Place the above raw materials in a mixer and mix at 380 r / min for 12 min to obtain 100 g of mixed raw materials. Then spray 10 g of pure water into the mixed raw materials and let it stand at 25℃ for 25 min to complete the pre-wetting treatment.

[0106] 2) Add 900 mL of pure water to the pre-wetted mixed raw materials and control the pH of the system to 6; first, extract at 52℃ and 240 r / min for 28 min by stirring; then raise the temperature to 60℃ and continue to extract by stirring for 42 min; after extraction, filter through a 200-mesh filter and collect the first filtrate; add 700 mL of pure water to the filter residue again and extract by stirring at 60℃ for 35 min, filter and collect the second extract, combine the two extracts to obtain a composite extract;

[0107] 3) After centrifuging the compound extract at 5000 r / min for 8 min, take the supernatant and cool it to 48℃. Then add 0.5 g of pectinase and 0.22 g of cellulase and hydrolyze at 48℃ for 35 min. Stir slowly at 100 r / min during the hydrolysis. After the hydrolysis is completed, heat at 85℃ for 5 min to inactivate the enzyme.

[0108] 4) After cooling the enzyme-inactivated extract to room temperature, centrifuge at 4000 r / min for 10 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain a clear extract;

[0109] 5) Place the clarified extract in a rotary evaporator and concentrate it under reduced pressure at -0.09 MPa. During the concentration process, the water bath temperature is controlled at 54℃ and the rotation speed is controlled at 80 r / min. Concentration is stopped when the relative density at 54℃ is 1.17~1.19. Then, it is rapidly cooled to below 30℃.

[0110] 6) Dissolve 0.15 g of tea polyphenols in 6 mL of pure water to obtain a tea polyphenol solution, add it to the tobacco extract obtained in step 5), stir at 40°C for 15 min, pack with nitrogen purging 3 times, seal, and store at 4°C in the dark to obtain tobacco extract.

[0111] Comparative Example 1

[0112] A method for preparing tobacco extract differs from Example 1 only in that it does not employ a gradient extraction process: after adding 900 mL of pure water to the mixed raw materials, it is directly extracted continuously at 250 r / min for 65 min at 60°C; the remaining process steps are the same as in Example 1.

[0113] Comparative Example 2

[0114] A method for preparing tobacco extract differs from Example 1 only in that it does not involve compound enzymatic hydrolysis; the compound extract is filtered and then directly clarified and concentrated under reduced pressure. The remaining process steps are the same as in Example 1.

[0115] Comparative Example 3

[0116] A method for preparing tobacco extract differs from Example 1 only in the vacuum concentration temperature: the water bath temperature is controlled at 65°C during vacuum concentration; the remaining process steps are the same as in Example 1.

[0117] Comparative Example 4

[0118] A method for preparing tobacco extract differs from Example 1 in that it uses only 100g of re-dried tobacco leaf fragments from the middle section as raw material, without adding bitter orange blossoms or honeysuckle; the remaining process steps are the same as in Example 1.

[0119] Comparative Example 5

[0120] A method for preparing tobacco extract differs from Example 1 in that it uses only 76g of re-dried tobacco leaf fragments from the middle section and 24g of bitter orange blossoms as raw materials; the remaining process steps are the same as in Example 1.

[0121] Comparative Example 6

[0122] A method for preparing tobacco extract, which differs from Example 1 only in that tea polyphenols are not added; the remaining process steps are the same as in Example 1.

[0123] Performance testing

[0124] The performance of the tobacco extracts from Examples 1-8 and Comparative Examples 1-6 was tested as follows:

[0125] 1) Sensory evaluation:

[0126] Nine professionals with experience in cigarette tasting conducted a sensory evaluation. Evaluation indicators included: floral characteristics, sweetness, aroma harmony, off-flavors, irritation, and aftertaste comfort. A 10-point scale was used for scoring, and the average score was taken.

[0127] 2) Extract yield test:

[0128] Weigh the obtained tobacco extract and calculate the extract yield according to the following formula:

[0129]

[0130] Where: m1 is the mass of the extract; m0 is the total mass of the raw materials. Each sample was measured in triplicate, and the average value was taken.

[0131] 3) GC-MS volatile aroma component detection:

[0132] The contents of volatile aroma components such as linalool, nerol, and phenethyl alcohol were detected by GC-MS, and the relative peak areas of the total volatile aroma components were calculated. Linalool, nerol, phenethyl alcohol, and geraniol were quantitatively analyzed using the external standard method. The relative peak areas of the total volatile aroma components were calculated using the peak area normalization method, and the sum of the peak areas of each volatile aroma component in the GC-MS total ion chromatogram represented the overall level of volatile aroma components. The chromatographic column was an HP-5MS quartz capillary column (30m × 0.25 mm × 0.25 μm); the carrier gas was high-purity helium at a flow rate of 1.0 mL / min; the injection volume was 1 μL; the temperature program was as follows: initial temperature 50℃ held for 2 min; increased to 180℃ at 5℃ / min; then increased to 250℃ at 10℃ / min and held for 5 min. Each sample was analyzed in triplicate.

[0133] 4) Stability test:

[0134] The tobacco extract obtained in Example 1 and the extract obtained in Comparative Example 6 were stored in sealed brown glass bottles at 25°C and 50% relative humidity for 30 days in the dark. The retention rate of total volatile aroma components was determined by GC-MS, and sensory evaluation was performed. The results are shown in Tables 1-3.

[0135] Table 1 Sensory evaluation results of each embodiment and comparative example

[0136]

[0137] Table 2 Performance test results of each embodiment and comparative example

[0138]

[0139] Table 3. Stability test results of Example 1 and Comparative Example 6

[0140]

[0141] As shown in Tables 1-3, the content of volatile floral aroma components such as linalool, nerol, phenylethyl alcohol, and geraniol in the tobacco extract obtained in the embodiments of the present invention is significantly higher than that in the comparative example. Furthermore, after employing gradient extraction and compound enzymatic hydrolysis, the relative peak area of ​​the total volatile aroma components significantly increased, indicating that the process of the present invention is beneficial for the release of aroma-producing components. Stability test results show that after adding tea polyphenol stabilizers, the retention rate of volatile aroma components after 30 days of storage is significantly improved, effectively slowing down the oxidative loss of aroma components.

[0142] Depend on Figure 2 It can be seen that the tobacco extract obtained in the embodiments of the present invention is significantly superior to the comparative example in terms of floral fragrance characteristics, sweetness, and aroma harmony.

[0143] This invention utilizes a synergistic blend of re-dried tobacco leaf fragments, bitter orange blossoms, and honeysuckle, combined with gradient extraction and complex enzymatic hydrolysis processes. This effectively enhances the aroma component content and sensory quality of Yunnan tobacco extract, and improves product storage stability through tea polyphenol stabilization treatment. It has significant industrial application value.

[0144] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method of preparing a cloud smoke extract, characterized by, Includes the following steps: 1) After mixing the re-drying fragments of tobacco leaves, bitter orange blossoms and honeysuckle, pre-moisten them to obtain the mixed raw materials; 2) Add pure water to the mixed raw materials obtained in step 1) and perform gradient temperature extraction, and perform a second extraction. Combine the extracts to obtain a composite extract. 3) Add pectinase and cellulase to the compound extract obtained in step 2) for enzymatic hydrolysis, and then inactivate the enzymes; 4) Centrifuge and filter the extract obtained after enzymatic hydrolysis in step 3) to obtain a clear extract; 5) After the clarified extract obtained in step 4) is concentrated under reduced pressure, tea polyphenols are added for stabilization treatment, and nitrogen is used for packaging to obtain Yunnan tobacco extract.

2. The process for the preparation of cloud smoke extract according to claim 1, characterized in that, In step 1), the mass ratio of the re-drying fragments of the middle tobacco leaves, bitter orange blossoms, and honeysuckle is 58~64:20~26:

16.

3. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 1), the bitter orange blossoms and honeysuckle are pulverized to 30 mesh, and the moisture content of the re-dried tobacco leaf fragments in the middle is controlled to be 10%~14%.

4. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 1), the pre-wetting treatment is as follows: spray 8-12 g of pure water onto 100 g of mixed raw materials and let it stand at 20-30°C for 20-30 min.

5. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 2), the gradient temperature extraction process is as follows: the first stage extraction temperature is 50~54℃ and the extraction time is 28~30 min; the second stage extraction temperature is 58~61℃ and the extraction time is 42~45 min; then the filter residue is extracted a second time at an extraction temperature of 58~61℃ for 35 min.

6. The process for the preparation of cloud smoke extract according to claim 1, characterized in that, In step 2), the pH of the system is controlled at 5.8~6.2 during the gradient extraction process.

7. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 3), the enzymatic hydrolysis process involves the following steps: pectinase is added at a rate of 0.4–0.55 g; cellulase is added at a rate of 0.22–0.25 g; the hydrolysis temperature is 46–48 °C; and the hydrolysis time is 35–42 min.

8. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 5), the vacuum concentration conditions are: vacuum degree of -0.09 to -0.088 MPa; water bath temperature of 52 to 55°C; and concentration to a relative density of 1.17 to 1.

19.

9. The method of claim 1, wherein the cloud smoke extract is prepared by the steps of: In step 5), the amount of tea polyphenols added is 0.1% to 0.15% of the mass of Yunnan tobacco extract, and the mixture is stirred at 40°C for 10 to 20 minutes.

10. The tobacco extract prepared by the method according to any one of claims 1-9.

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