A bod5 detection device and detection method

CN122754504APending Publication Date: 2026-09-15SHENZHEN WATER GRP CO LTD
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Patent Information

Application Number
CN202611150943.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-07-31
Publication Date
2026-09-15

AI Technical Summary

Technical Problem

[0006]本发明要解决的技术问题在于克服现有技术流程割裂、依赖人工、效率低下且易出错的不足,目的在于提供一种智能化水平更高、数据测量更精准、效率更高的BOD5检测设备

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Abstract

The application belongs to the technical field of water quality analysis and detection, and discloses a BOD5 detection device and a detection method. The BOD5 detection device drives an integrated detection module to move and position in a three-dimensional space through a multi-axis motion mechanism. The module integrates a plurality of functional units for performing different detection steps, including liquid processing, dissolved oxygen detection, automatic opening and closing of a bottle cap, and sample information identification. According to the identification information, the device automatically controls the functional units to sequentially perform a series of operations, such as cap opening, liquid filling and extraction, dissolved oxygen measurement, and cap closing, on the sample container according to a preset program. The application realizes full-process and batch automatic BOD5 determination from sample identification to data acquisition, completely replaces the traditional manual operation mode, and significantly improves the detection efficiency, consistency and accuracy.
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Description

Technical Field

[0001] This invention belongs to the field of water quality analysis and testing technology, specifically, it relates to a BOD5 testing device and testing method. Background Technology

[0002] Five-day biochemical oxygen demand (BOD5) is one of the core indicators for evaluating the degree of organic pollutant pollution in water bodies. Its standard determination process is cumbersome and time-consuming. Traditional methods, based on national standards, require a series of manual operations: appropriate dilution or inoculation of water samples, pH adjustment, dispensing into dissolved oxygen bottles, sealing and incubating for five days, and then measuring the dissolved oxygen concentration in the water samples before and after incubation.

[0003] Currently, laboratories generally still use manual or semi-automatic operation modes. Operators need to manually open and close dozens of dissolved oxygen bottles, use pipettes or graduated cylinders to accurately add and mix reagents, and measure each bottle individually using a dissolved oxygen meter. This method has significant drawbacks: First, manual operation is inefficient and difficult to handle large-scale sample testing, artificially lengthening the testing cycle; second, the repetitive labor intensity is high, and errors are easily caused by operator fatigue, such as adding the wrong reagent, improper bottle cap sealing, and mixing up sample numbers, seriously affecting the accuracy and reliability of the data; finally, differences in the techniques of different operators can also introduce systematic errors, resulting in poor repeatability of test results. To improve efficiency, some automated auxiliary equipment has emerged in existing technologies, such as automatic diluents and dissolved oxygen meters, but these can only replace single operational steps. Key connections such as sample transfer between different devices and bottle cap opening and closing still require manual intervention, resulting in fragmented processes, limited efficiency improvements, and the continued risk of errors.

[0004] Therefore, developing a device and method to achieve unattended and automated operation of the entire BOD5 measurement process is of great significance for improving the intelligence level of water quality testing laboratories and ensuring data quality.

[0005] In view of the above, this application is hereby submitted. Summary of the Invention

[0006] The technical problem to be solved by this invention is to overcome the shortcomings of existing technologies, such as fragmented processes, reliance on manual labor, low efficiency, and susceptibility to errors. The aim is to provide a BOD5 detection device with a higher level of intelligence, more accurate data measurement, and higher efficiency.

[0007] Another objective of this invention is to provide a BOD5 detection method applied to the aforementioned BOD5 detection equipment, which achieves standardization and unmanned operation of the detection process through programmed control.

[0008] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is: a BOD5 detection device, comprising: A multi-axis motion mechanism, which is capable of positioning and moving in three-dimensional space; A detection module is mounted on the multi-axis motion mechanism and is driven to move by it; A sample carrier device, which is used to carry a sample container; The detection module includes: Liquid handling unit, used for adding or extracting liquid into sample containers; Dissolved oxygen detection unit, used to detect the dissolved oxygen content in the sample container; A cap operation unit is used to open or close the cap of the sample container; the cap operation unit, dissolved oxygen detection unit, and liquid handling unit are all independently driven components. An information identification unit is used to identify the identification information on the sample carrier or sample container.

[0009] According to one embodiment of the present invention, the multi-axis motion mechanism is a three-axis gantry assembly, including two X-axis arranged in parallel in a horizontal plane, a Y-axis mounted on the moving parts of the two X-axis and moving in a vertical direction, and a Z-axis assembly mounted on the moving parts of the Y-axis and moving in a vertical direction. The detection module is mounted on the Z-axis assembly.

[0010] According to one embodiment of the present invention, the liquid processing unit includes a liquid injection tube for adding or extracting liquid; The detection module also includes an inoculation solution tube for adding the inoculation solution and a propylene thiourea tube for adding propylene thiourea; the injection tube, inoculation solution tube, propylene thiourea tube, dissolved oxygen detection unit and bottle cap operation unit are each installed through an independent vertical drive mechanism.

[0011] According to one embodiment of the present invention, the independent vertical drive mechanism is a miniature linear module; The Z-axis assembly includes five Z-axis axes that drive the injection tube, inoculation tube, propylene thiourea tube, dissolved oxygen detection unit, and bottle cap operation unit, respectively.

[0012] According to one embodiment of the present invention, the micro linear module includes a motor, a lead screw driven by the motor, and a nut slider fitted on the lead screw; The nut slider is equipped with balls and a ball circulation device that cooperate with the screw spiral groove.

[0013] According to one embodiment of the present invention, the bottle cap operating unit includes two fingers that can be opened and closed for gripping the bottle cap, and a finger drive motor for driving the fingers to open and close.

[0014] According to one embodiment of the present invention, the information identification unit is an automatic barcode scanner; The information recognition unit includes a camera, an image processor, and a storage device, and is fixedly installed on the bottle cap operation unit.

[0015] The present invention also provides a BOD5 detection method, applied to the above-mentioned BOD5 detection equipment, comprising the following steps: The information recognition unit identifies the identification information on the sample carrier device and obtains the sequence of operations to be performed for each of the multiple sample containers. Based on the operation sequence, perform the following automated operations on the current target sample container: The control cap operation unit opens the cap of the target sample container; According to the instructions of the operation sequence, the liquid handling unit and / or dissolved oxygen detection unit are controlled to perform corresponding liquid operations or dissolved oxygen detection in sequence; After completing all the specified operations, control the cap operation unit to close the cap of the sample container; Repeat the above automated operation until all sample containers have been processed.

[0016] According to one embodiment of the present invention, the liquid processing unit includes a plurality of independently driven functional tubes, and in the step of sequentially controlling the execution according to the operation sequence instructions, only the functional tube to be operated is lowered into the sample container, while the other functional tubes remain in the retracted state.

[0017] According to one embodiment of the present invention, the information identification unit identifies the identification information by identifying a QR code; The obtained operation sequence information includes at least whether inoculation is required, whether allyl thiourea needs to be added, and the order of each operation step.

[0018] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art: 1) Full-process automation: For the first time, multiple steps in BOD5 testing that require manual intervention (opening the cap, adding multiple reagents, measuring, and closing the cap) are integrated into a single device for continuous and automated completion, achieving true "sample in, data out," greatly freeing up manpower and significantly improving testing throughput; 2) Error prevention and traceability: By automatically identifying sample labels and binding them to preset programs, problems such as sample confusion and reagent addition errors that are prone to occur during manual operation are completely eliminated. All operations are automatically recorded by the system, ensuring the standardization of the testing process and the integrity and traceability of the data. 3) High precision and high consistency: Mechanical automation eliminates manual errors and individual variations inherent in human operation. Precision motion control ensures consistency in key parameters such as liquid volume and electrode insertion depth, thereby significantly improving the accuracy and repeatability of test results and guaranteeing data quality.

[0019] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0020] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings: Figure 1 This is a partial structural schematic diagram of a BOD5 detection device according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the detection module in an embodiment of the present invention.

[0021] Description of main components in the diagram: 1. X1 axis; 2. X2 axis; 3. Y-axis; 4. Protective outer cover; 5. Z1 axis; 6. Z2 axis; 7. Z3 axis; 8. Z4 axis; 9. Propylene-based thiourea tubing; 10. Inoculation solution tube; 11. Injection tube; 12. Dissolved oxygen detection unit; 13. Bottle cap operation unit; 14. Information recognition unit; 15. Z5 axis.

[0022] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0024] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0025] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0026] like Figures 1 to 2 As shown, the BOD5 detection device of the present invention includes: A multi-axis motion mechanism, which is capable of positioning and moving in three-dimensional space; A detection module is mounted on the multi-axis motion mechanism and is driven to move by it; A sample carrier device, which is used to carry a sample container; The detection module includes: Liquid handling unit, used for adding or extracting liquid into sample containers; Dissolved oxygen detection unit, used to detect the dissolved oxygen content in the sample container; The cap operation unit 13 is used to open or close the cap of the sample container; the cap operation unit 13, the dissolved oxygen detection unit, and the liquid processing unit are all independently driven components. The information identification unit 14 is used to identify the identification information on the sample carrier or sample container.

[0027] In this invention, by setting up the multi-axis motion mechanism, the detection module and the sample carrying device in a coordinated manner, a physical platform capable of fully automated serialization of batch sample containers is constructed, which is the foundation for realizing automated operation.

[0028] The core design concept of the BOD5 detection device of the present invention is to use a mechanical platform that can move precisely in three-dimensional space, carrying a multi-functional tool head (detection module) that integrates all necessary functions, to perform unattended serialized operations on multiple standard dissolved oxygen bottles arranged on a fixed carrier plate (sample carrying device).

[0029] Please see the appendix Figure 1 and attached Figure 2 In one specific embodiment of this example, the multi-axis motion mechanism is a three-axis gantry assembly, including two X-axis arranged in parallel in the horizontal plane, a Y-axis 3 mounted on the moving parts of the two X-axis and moving in the vertical direction, and a Z-axis assembly mounted on the moving parts of the Y-axis 3 and moving in the vertical direction. The detection module is mounted on the Z-axis assembly.

[0030] In this invention, by setting up the three-axis gantry assembly, the detection module is provided with a wide range, high precision, stable and reliable motion capability in three-dimensional space, ensuring that each operation can be accurately positioned to each sample container.

[0031] More specifically, the multi-axis motion mechanism includes: two linear guide rails (X1 axis 1 and X2 axis 2) arranged parallel to each other in the horizontal plane along the length of the equipment, forming an X-axis motion pair; a crossbeam (Y-axis 3) spanning the two X-axis guide rails and perpendicular to the X-axis, forming a Y-axis motion pair, the two ends of the crossbeam being connected to the two X-axis guide rails through sliders, so that the entire crossbeam can move synchronously and stably along the X-axis direction; A lifting mechanism vertically mounted on the aforementioned Y-axis beam 3 constitutes the Z-axis kinematic pair. Each kinematic pair is powered by a servo motor or stepper motor, which drives a high-precision ball screw to rotate via a coupling, thereby converting the rotational motion of the motor into the precise linear motion of the load-bearing components.

[0032] This gantry structure features high rigidity, strong load-bearing capacity, large range of motion, and high positioning accuracy, making it ideal for applications requiring precision operations at multiple workstations within a large table area.

[0033] In one specific embodiment of this example, the detection module is installed at the Z-axis end of the multi-axis motion mechanism, and a protective cover 4 is provided on the outside of the detection module.

[0034] Please see the appendix Figure 1 and attached Figure 2 In one specific embodiment of this example, the liquid processing unit includes a liquid injection pipe 11 for injecting or extracting liquid. The detection module also includes an inoculation solution tube 10 for adding inoculation solution and an propylene thiourea tube 9 for adding propylene thiourea; the injection tube 11, the inoculation solution tube 10, the propylene thiourea tube 9, the dissolved oxygen detection unit and the bottle cap operation unit 13 are respectively installed through independent vertical drive mechanisms.

[0035] In this invention, by setting up independent pipelines for extracting liquid, inoculating and adding inhibitors respectively, and installing them together with the dissolved oxygen detection unit and the bottle cap operation unit 13 through an independent vertical drive mechanism, functional modularization and on-demand independent calling are realized for different sample needs, avoiding functional interference, which is the key to high integration.

[0036] The "detection module" is the key component that directly performs all detection operations. It is installed as a single unit at the Z-axis end of the aforementioned multi-axis motion mechanism.

[0037] The detection module contains the following specific units: Liquid handling unit: This unit is not a single pipeline, but is specifically divided into three independent functional pipelines according to the different liquid handling requirements in BOD5 detection: (a) The injection tube 11 is connected to an external dilution water, pure water or aeration gas source, and is responsible for performing sample dilution, water replenishment or pre-aeration and oxygenation. (b) Inoculation solution tube 10, connected to a specific microbial agent storage tank, is responsible for introducing degrading microorganisms into the sample to be inoculated; (c) Propylene thiourea tubing 9, connected to the inhibitor reservoir, is responsible for adding propylene thiourea solution to the sample requiring inhibition of nitration. Each tubing is equipped with an independent micro-precision pump or valve assembly to control the precise addition and extraction of reagents.

[0038] Dissolved oxygen detection unit: This unit is specifically a membrane dissolved oxygen electrode (dissolved oxygen detection unit) with an automatic stirring function. It integrates a micro magnetic stirrer, which can quickly agitate the sample solution during measurement, thereby renewing the water on the electrode surface and obtaining a stable and accurate dissolved oxygen reading.

[0039] Bottle cap operation unit 13: This unit is specifically a clamping and screwing mechanism. It includes a pair of arc-shaped or semi-circular gripping fingers that can be driven by a micro motor to open and close. The inner shape of the gripping fingers matches the outer contour of the threaded cap of a standard dissolved oxygen bottle. The entire mechanism can reciprocate within a small stroke to simulate the action of a human hand screwing the cap in and out.

[0040] Information recognition unit 14: The unit is preferably a one-dimensional or two-dimensional barcode scanner (barcode scanner), the core of which is a small industrial camera and image decoding circuit. It is physically fixed to the housing of the bottle cap operation unit 13, so that its field of view can naturally cover the preset marking area on the bottle or tray before or after the cap opening operation. The one-dimensional or two-dimensional barcode in the marking area is associated with the unique ID of each sample container and the customized detection procedure (such as "dilution required + inoculation required + dissolved oxygen measurement required").

[0041] In one specific implementation of this embodiment, the independent vertical drive mechanism is a miniature linear module; The Z-axis assembly includes five Z-axis that drive the injection tube 11, the inoculation tube 10, the propylene thiourea tube 9, the dissolved oxygen detection unit, and the cap operation unit 13, respectively.

[0042] In this invention, by setting the micro linear module as an independent vertical drive mechanism, precise and reliable vertical feed motion control is provided for each functional component, which is the basic mechanical structure for realizing "independent drive" and "precise operation".

[0043] Each of the aforementioned functional lines (injection tube 11, inoculation tube 10, and propylene-based thiourea tube 9), dissolved oxygen electrode, and cap clamping and screwing mechanism is equipped with an independent, small "micro linear module" as its dedicated vertical drive mechanism, corresponding to Z3 axis 7 (driving the Z-axis of injection tube 11), Z2 axis 6 (driving the Z-axis of inoculation tube 10), Z1 axis 5 (driving the Z-axis of propylene-based thiourea tube 9), Z4 axis 8 (driving the Z-axis of dissolved oxygen detection unit 12), and Z5 axis 15 (driving the Z-axis of cap operating unit 13). Each module includes a micro motor, a vertically mounted lead screw, and a nut slider that mates with the lead screw. The functional components are mounted on the corresponding sliders. Therefore, the control system can individually control the motor rotation of any one module, driving its corresponding functional component (such as inoculation tube 10) to extend downwards and enter the dissolved oxygen bottle below for operation, while other functional components remain retracted inside the module housing, without interfering with each other.

[0044] This design enables a dense arrangement of functions and on-demand access, which is key to integration.

[0045] In one specific embodiment of this example, the micro linear module includes a motor, a lead screw driven by the motor, and a nut slider mounted on the lead screw; The nut slider is equipped with balls and a ball circulation device that cooperate with the screw helical groove; the balls form a closed loop rolling through the ball circulation device integrated inside the nut (which makes the balls form a closed loop path inside the nut).

[0046] In this invention, by setting a precision lead screw and nut pair including ball bearings and a ball bearing circulation device, the high precision, low friction and long life of the micro linear module motion are ensured, thereby guaranteeing the long-term stability and repeatability of the entire equipment.

[0047] In one specific embodiment of this example, the bottle cap operation unit 13 includes two fingers that can be opened and closed for gripping the bottle cap, and a finger drive motor for driving the fingers to open and close.

[0048] In this invention, by setting up the clamping and screwing mechanism composed of the fingers and the finger-driven motor, the action of opening and closing the bottle cap by a human hand is reliably simulated, realizing the automation of the sample container sealing process and solving the key bottleneck in the automation process.

[0049] Please see the appendix Figure 2 In one specific embodiment of this example, the information identification unit 14 is an automatic barcode scanner; The information recognition unit 14 includes a camera, an image processor, and a storage device, and is fixedly installed on the bottle cap operation unit 13; During operation, the camera captures images of the identification marks, which are then decoded by the image processor. The identified information (such as the sample's unique code) is then sent to the control system. Based on this information, the control system retrieves a preset detection operation sequence from the storage device that is bound to the code.

[0050] In this invention, by setting the automatic barcode scanner fixed on the bottle cap operation unit 13, the sample information can be read naturally and efficiently during or before the opening or closing of the cap, thus realizing the accurate binding of the detection command and the physical sample.

[0051] The "sample carrier" is specifically a positioning tray or rack capable of holding multiple (e.g., 18) standard dissolved oxygen bottles. Each bottle slot has a unique graphic identifier (such as a QR code) below or to the side, which is linked to the complete testing protocol for that sample in the database.

[0052] The present invention also provides a BOD5 detection method applied to the above-mentioned BOD detection equipment, the BOD5 detection method comprising step S1: The information recognition unit identifies the identification information on the sample carrier device and obtains the sequence of operations to be performed for each of the multiple sample containers. Based on the operation sequence, perform the following automated operations on the current target sample container: The control cap operation unit opens the cap of the target sample container; According to the instructions of the operation sequence, the liquid handling unit and / or dissolved oxygen detection unit are controlled to perform corresponding liquid operations or dissolved oxygen detection in sequence; After completing all the specified operations, control the cap operation unit to close the cap of the sample container; Repeat the above automated operation until all sample containers have been processed.

[0053] In this invention, by setting the method of prioritizing information recognition and driving each independent functional unit to operate sequentially according to a preset sequence, the standardization and procedural operation of the detection process are realized, ensuring the correctness of the logic and the integrity of the steps in batch processing.

[0054] In one specific embodiment of this example, the liquid processing unit includes multiple independently driven functional tubes. In the step of sequentially controlling the execution according to the operation sequence instructions, only the functional tube to be operated is lowered into the sample container, while the other functional tubes remain in the retracted state.

[0055] In this invention, by controlling the descent of only the functional tube that needs to be operated at the moment, physical collisions or cross-contamination that may occur between multiple functional components in the narrow bottle opening are effectively avoided, thereby improving the safety and reliability of automated operation.

[0056] In one specific implementation of this embodiment, the information recognition unit identifies the identification information by recognizing a QR code; The obtained operation sequence information includes at least whether inoculation is required, whether allyl thiourea needs to be added, and the order of each operation step.

[0057] In this invention, by setting up a sequence of instructions that uses QR codes as carriers to transmit key information such as vaccination, inhibitor requirements, and operation order, a precise and traceable digital work order is provided for the fully automated operation of the equipment.

[0058] More specifically, the BOD5 detection method includes the steps S0-S1-S2.

[0059] Step S0, Initialization and Recognition: The operator dispenses the pre-treated samples (such as initial dilution and pH adjustment) into dissolved oxygen bottles and places them in the fixed positions of the sample carrier. After starting the equipment, the control system first instructs the multi-axis motion mechanism to move the detection module above the tray marking area, and then reads the total code of the tray or the individual code of each bottle sequentially using an automatic barcode scanner. The decoded information is transmitted to the equipment's control system. The control system then calls the preset detection program based on the sample ID (e.g., for bottle #3, the operation sequence is: open the cap -> add 10 mL of dilution water -> add 2 mL of inoculum -> measure the initial dissolved oxygen -> close the cap).

[0060] Step S1, Serialization Automated Operation: The equipment automatically cycles from the first bottle position (this part is a detailed description of the steps listed above).

[0061] a. Positioning and Opening: The multi-axis motion mechanism precisely moves the entire detection module to directly above the target dissolved oxygen cylinder according to the program. Then, the control system first activates the dedicated linear module of the cap operation unit, driving the cap clamping mechanism to descend to the cap position, where the clamping fingers close and tighten the cap. Then, the module moves in the reverse direction, unscrewing the cap and raising it to a safe height.

[0062] b. Sequential Function Operations: Based on the bottle's program instructions, the control system sequentially schedules the relevant functional components. For example, if dilution water needs to be added, the multi-axis motion mechanism moves the injection tube to the bottle opening, then activates the corresponding linear module to lower it to a suitable depth below the liquid surface, and then activates the external pump valve to complete the injection. After completion, the module is raised and retracted. Next, the inoculum tube is scheduled for injection according to the same logic. If dissolved oxygen needs to be measured, the dissolved oxygen electrode is lowered to the measurement position, the built-in agitator is activated, and data is read. After completion, it is retracted. During all operations, unused components remain stationary.

[0063] c. Closing and Transfer: After all preset steps for the bottle are completed, the control system again lowers the bottle cap operating unit to accurately tighten the clamped bottle cap to the bottle opening, then releases the clamping fingers and retracts it. At this point, the fully automated processing of a single sample container is complete. The multi-axis motion mechanism then drives the detection module to move to the next bottle to be processed, repeating steps a to c.

[0064] S2, Batch processing complete: The equipment processes all dissolved oxygen cylinders on the tray sequentially in this manner, requiring no manual intervention throughout the process. All operation logs and measurement data are automatically stored, and test reports are generated.

[0065] Through the implementation of the specific equipment and methods described above, this invention successfully transforms a classic environmental testing method that was originally highly dependent on skilled technicians, cumbersome and error-prone into an efficient, accurate and reliable automated process, demonstrating significant technological advancement and practical value.

[0066] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. The implementation schemes in the above embodiments can also be further combined or replaced. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A BOD5 detection device, characterized in that, include: A multi-axis motion mechanism, which is capable of positioning and moving in three-dimensional space; A detection module is mounted on the multi-axis motion mechanism and is driven to move by it; A sample carrier device, which is used to carry a sample container; The detection module includes: Liquid handling unit, used for adding or extracting liquid into sample containers; Dissolved oxygen detection unit, used to detect the dissolved oxygen content in the sample container; A cap operation unit is used to open or close the cap of the sample container; the cap operation unit, dissolved oxygen detection unit, and liquid handling unit are all independently driven components. An information identification unit is used to identify the identification information on the sample carrier or sample container.

2. The BOD5 detection device according to claim 1, characterized in that, The multi-axis motion mechanism is a three-axis gantry assembly, including two X-axis arranged in parallel in the horizontal plane, a Y-axis mounted on the moving parts of the two X-axis and moving in the vertical direction, and a Z-axis assembly mounted on the moving parts of the Y-axis and moving in the vertical direction. The detection module is mounted on the Z-axis assembly.

3. The BOD5 detection device according to claim 2, characterized in that, The liquid processing unit includes a liquid injection pipe for adding or extracting liquid; The detection module also includes an inoculation solution tube for adding the inoculation solution and a propylene thiourea tube for adding propylene thiourea; the injection tube, inoculation solution tube, propylene thiourea tube, dissolved oxygen detection unit and bottle cap operation unit are each installed through an independent vertical drive mechanism.

4. The BOD5 detection device according to claim 3, characterized in that, The independent vertical drive mechanism is a miniature linear module; The Z-axis assembly includes five Z-axis axes that drive the injection tube, inoculation tube, propylene thiourea tube, dissolved oxygen detection unit, and bottle cap operation unit, respectively.

5. A BOD5 detection device according to claim 4, characterized in that, The miniature linear module includes a motor, a lead screw driven by the motor, and a nut slider mounted on the lead screw; The nut slider is equipped with balls and a ball circulation device that cooperate with the screw spiral groove.

6. The BOD5 detection device according to claim 1, characterized in that, The bottle cap operating unit includes two fingers that can be opened and closed for gripping the bottle cap, and a finger drive motor for driving the fingers to open and close.

7. The BOD5 detection device according to claim 1, characterized in that, The information recognition unit is an automatic barcode scanner; The information recognition unit includes a camera, an image processor, and a storage device, and is fixedly installed on the bottle cap operation unit.

8. A BOD5 detection method, applied to the BOD5 detection equipment according to any one of claims 1-7, characterized in that, Including the following steps: The information recognition unit identifies the identification information on the sample carrier device and obtains the sequence of operations to be performed for each of the multiple sample containers. Based on the operation sequence, perform the following automated operations on the current target sample container: The control cap operation unit opens the cap of the target sample container; According to the instructions of the operation sequence, the liquid handling unit and / or dissolved oxygen detection unit are controlled to perform corresponding liquid operations or dissolved oxygen detection in sequence; After completing all the specified operations, control the cap operation unit to close the cap of the sample container; Repeat the above automated operation until all sample containers have been processed.

9. A BOD5 detection method according to claim 8, characterized in that, The liquid processing unit includes multiple independently driven functional tubes. In the step of sequentially controlling the execution according to the operation sequence instructions, only the functional tube that needs to be operated is lowered into the sample container, while the other functional tubes remain in the retracted state.

10. A BOD5 detection method according to claim 8, characterized in that, The specific method by which the information recognition unit identifies the identification information is by recognizing a QR code; The obtained operation sequence information includes at least whether vaccination is required, whether allyl thiourea needs to be added, and the order of each operation step.