High flash point mustard oil and method of making same

Abstract

This invention discloses a high flash point mustard oil and its preparation method, relating to the field of antibacterial agent technology. It comprises the following components by weight: isopropyl myristate: 7-13 parts, mustard oil: 78-82 parts, and triethyl citrate: 7-13 parts. The product is prepared by mixing isopropyl myristate and triethyl citrate at room temperature, then adding mustard oil and stirring continuously, followed by allowing it to stand and mature. The product has a stable flash point of 62℃, solving the problems of low flash point (approximately 36.3℃) and flammability / explosiveness of traditional mustard oil, significantly reducing safety risks during packaging, transportation, and storage. Simultaneously, it retains the broad-spectrum volatile antibacterial properties of mustard oil, inhibiting the growth and reproduction of bacteria and mold in the environment without direct contact, making it suitable for various scenarios such as home use, vehicle use, and fresh vegetable storage and preservation. The product components are safe, non-toxic, and environmentally friendly. The preparation process is simple and controllable, and the finished product has stable performance, making it suitable for industrial-scale mass production and possessing high practical value and promising prospects for promotion.

Description

Technical Field

[0001] This invention relates to the field of antibacterial agent technology, specifically to a high flash point mustard oil and its preparation method. Background Technology

[0002] Mustard oil is a common condiment in daily life, known for its distinctive pungent aroma. It possesses certain antibacterial and anti-inflammatory properties. The isothiocyanates in mustard oil have strong antibacterial capabilities. Applying a suitable amount to the skin can inhibit the growth and reproduction of various bacteria and fungi. It can also provide some relief for mild skin inflammations, such as folliculitis and small boils caused by bacterial infections, reducing inflammatory responses and promoting inflammation resolution.

[0003] In the field of health and wellness, mustard oil has strong volatility. When it evaporates / is sprayed into the air, it can also inhibit the growth and reproduction of bacteria and fungi. Therefore, mustard oil can also be used as the base of antibacterial agents. In special applications, such as in cars and air conditioners, it can inhibit the growth and reproduction of various bacteria such as mold and E. coli, and reduce odors in cars or air conditioners. In agriculture, mustard oil can be used as an ingredient in insecticides. As an insecticide, it has many advantages, as it can control a variety of pests, especially nematodes. In addition, it is highly volatile, so its retention time on crops is very short, leaving virtually no residue, which is more environmentally friendly.

[0004] However, mustard oil has a very low flash point, typically around 36.3°C. Due to its low flash point, its packaging, transportation, and storage require special methods; otherwise, it is highly flammable. Therefore, the packaging, transportation, and storage of mustard oil must take its flash point into account and take special precautions to prevent accidents. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a high flash point mustard oil and its preparation method, so as to improve the flash point of the mustard oil and facilitate its packaging, transportation and storage.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution: a high flash point mustard oil, composed of the following components in parts by weight: isopropyl myristate: 7-13 parts, mustard essential oil: 78-82 parts, and triethyl citrate: 7-13 parts.

[0007] Preferably, the high flash point mustard oil is composed of the following components in parts by weight: isopropyl myristate: 7 parts, mustard oil: 78 parts, and triethyl citrate: 7 parts.

[0008] Preferably, the high flash point mustard oil is composed of the following components in parts by weight: isopropyl myristate: 10 parts, mustard oil: 80 parts, and triethyl citrate: 10 parts.

[0009] Preferably, the high flash point mustard oil is composed of the following components in parts by weight: isopropyl myristate: 13 parts, mustard oil: 82 parts, and triethyl citrate: 13 parts.

[0010] The method for preparing the high flash point mustard oil includes the following steps: S1. According to the weight ratio, add isopropyl myristate and triethyl citrate into the reaction vessel, and stir evenly for more than 30 minutes at room temperature of 25°C and stirring speed of 280-320 rpm to obtain a mixed excipient liquid. S2. Add the corresponding weight of mustard oil to the mixed excipient liquid obtained in step S1, and stir continuously at a stirring speed of 280-320 rpm for 60 minutes until the material is evenly mixed. S3. After stirring evenly, let the mixture stand for 24 hours to mature, and finally bottle it to obtain high flash point mustard oil.

[0011] Preferably, the high flash point mustard oil in step S3 has a flash point of 62°C and a broad-spectrum antibacterial effect. It can inhibit the growth and reproduction of bacteria and mold in the space through its own volatility and can achieve antibacterial effect without direct contact with objects.

[0012] The beneficial effects of this invention are as follows: By precisely proportioning its components, this invention achieves a stable flash point of 62℃, effectively solving the problems of low flash point and flammability / explosiveness of traditional mustard oil. This significantly improves the safety of storage, transportation, and use, simplifies storage and transportation conditions, and is suitable for large-scale distribution. Simultaneously, the scientifically and rationally formulated ratio overcomes the drawbacks of excessive excipients reducing antibacterial effects or insufficient excipients failing to raise the flash point, thus balancing excellent antibacterial performance with high flash point characteristics. This product is a broad-spectrum volatile antibacterial agent that inhibits the growth of bacteria and mold in the environment without contact with objects, providing comprehensive antibacterial protection and convenient use. The product components are safe, non-toxic, and environmentally friendly, with no side effects on the human body, and can be widely used in home, automotive, and vegetable storage and preservation scenarios. Furthermore, the preparation process of this invention is simple, parameters are controllable, the finished product has stable performance and good batch consistency, making it suitable for industrial mass production and possessing extremely high practical value and promising prospects for promotion. Detailed Implementation

[0013] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0014] Example 1: This example provides a high flash point mustard oil, which is composed of the following components in parts by weight: 7 parts isopropyl myristate, 78 parts mustard oil, and 7 parts triethyl citrate.

[0015] The preparation method includes the following steps: S1. According to the above weight ratio, isopropyl myristate and triethyl citrate are added to a reaction vessel and stirred continuously for more than 30 minutes at room temperature of 25°C and stirring speed of 280 rpm to obtain a uniform mixed excipient liquid; S2. 78 parts of mustard oil are added to the mixed excipient liquid and stirred continuously at a constant speed of 280 rpm for 60 minutes to ensure that all components are completely mixed and uniform; S3. The mixed material is allowed to stand and mature for 24 hours. After maturation, it is bottled to obtain the finished high flash point mustard oil.

[0016] The mustard oil product prepared in this embodiment has a stable flash point of 62℃ and excellent broad-spectrum antibacterial properties. Relying on its own volatility, it can effectively inhibit the growth and reproduction of bacteria and mold in the space environment. It can achieve all-area antibacterial effect without direct contact with objects. The product is safe, non-toxic, and environmentally friendly, and is suitable for scenarios such as automobiles, homes, and the storage and preservation of fresh vegetables.

[0017] Example 2: This example provides a high flash point mustard oil, which is composed of the following components in parts by weight: 10 parts isopropyl myristate, 80 parts mustard oil, and 10 parts triethyl citrate.

[0018] The preparation method is exactly the same as in Example 1: Isopropyl myristate and triethyl citrate are stirred at 25°C and 300 rpm for more than 30 minutes to prepare a mixed excipient liquid; the corresponding weight parts of mustard oil are added and stirred at 300 rpm for 60 minutes until well mixed; after standing and aging for 24 hours, the product is bottled to obtain the finished product.

[0019] This embodiment represents the optimal formulation, with the best synergistic effect among the components. The finished product maintains a stable flash point of 62℃, demonstrating excellent flash point enhancement. Simultaneously, it maximizes the preservation of the antibacterial activity of mustard oil, avoiding the antibacterial effect degradation caused by excessive excipients. The product exhibits uniform volatility, a broad antibacterial spectrum, and stable performance, making it suitable for routine antibacterial use in various scenarios, resulting in optimal overall effectiveness.

[0020] Example 3: This example provides a high flash point mustard oil, which is composed of the following components in parts by weight: 13 parts isopropyl myristate, 82 parts mustard oil, and 13 parts triethyl citrate.

[0021] The preparation process parameters differ from those in Example 1 in that the stirring speed is 320 rpm, and the finished product is prepared by step stirring, constant temperature and constant speed mixing, static curing and then canning.

[0022] In this example, the auxiliary material ratio is the maximum ratio within the range, the flash point of the finished product is stably maintained at 62°C, the physical and chemical stability is excellent, and the safety during storage and transportation is extremely high. Under the premise of the maximum addition amount of auxiliary materials, the product still maintains good broad-spectrum bacteriostatic ability, with no obvious attenuation of bacteriostatic effect, balancing both high flash point characteristics and basic bacteriostatic performance, and is suitable for harsh use and storage and transportation scenarios such as high temperature and long-distance transportation.

[0023] Example 4: Bacteriostatic Test 1. Culture media: nutrient agar medium, Sabouraud's agar medium 2. Instruments used: biochemical incubator (No.: QFM-B-P016 / QFM-B-P015), biological safety cabinet (No.: QFM-B-P005), low-temperature thermostatic bath (No.: QFM-B-P014).

[0024] 3. Test Results Remarks: Effect evaluation criteria: when the bacteriostatic rate is ≥90%, the sample has a strong bacteriostatic effect on the tested strain; when the bacteriostatic rate is between 50% and 90%, the sample has a bacteriostatic effect on the tested strain.

[0025] Example 5: Acute Inhalation Toxicity Test (1) Apparatus and Animals 1. Test sample: bacteriostatic module.

[0026] 2. Experimental animals and feeding conditions: Experimental animals: 20 SPF-grade KM mice, half male and half female, with a body weight of 18 g to 22 g, provided by Hunan Sila Jingda Experimental Animal Co., Ltd., the production license number of experimental animals is: SCXK (Xiang) 2021-0002, and the quality certification number is: 430727241103651385. The animals were quarantined for 4 days in the laboratory animal room environment before the test.

[0027] Feeding conditions: The feed is provided by Keao Xieli (Tianjin) Feed Co., Ltd., the production license number is SCXK (Jin) 2020-0004, and the quality certification number is 1209162400035975. The temperature of the animal room is 20°C~26°C, and the relative humidity is 40%~70%. 12-hour lighting and 12-hour darkness.

[0028] (2) Methods 1. Testing basis: *Technical Specification for Disinfection* (2002 edition) 2.3.2 Acute inhalation toxicity test.

[0029] 2. Preparation of test substance: the original sample is used as it is.

[0030] 3. Exposure Method: A static exposure method was used. All animals were included in the exposure group, 20 animals per group, half male and half female. The experimental animals were placed in a sealed container (exposure chamber) of a fixed volume. The weighed test substance was placed in the exposure chamber to allow it to evaporate and reach the required concentration for the experiment. The exposure time was 2 hours. The volume of the exposure chamber was sufficient to provide at least 3L of air per exposed mouse per hour. After exposure, the remaining amount of test substance was weighed, and the actual exposure concentration was calculated to be 10185.19 mg / m³. 3 .

[0031] 4. Observation and Evaluation: Each animal should be observed at least once within 30 minutes of exposure, and the onset and disappearance times of toxic signs and the time of death should be observed and recorded. On day 1 of exposure, the toxic manifestations and mortality of the experimental animals should be observed regularly, followed by daily observation for 14 days. Animals that die during the observation period or are euthanized at the end of the observation period should be grossly dissected and visually examined. If any abnormalities are found in tissues or organs, further histopathological examination should be performed. If mice die during the experiment, the dosage should be reduced or multiple dosages should be designed for repeated testing; if no mice die, the acute inhalation LC50 of the test substance in mice is considered to be greater than the exposure concentration. The toxicity of the test substance should be evaluated according to the "Evaluation Provisions" in section 2.3.2.4 of the "Disinfection Technical Specifications" (2002 edition).

[0032] (III) Test Results No abnormal symptoms were observed in the animals during the exposure period and the 14-day observation period. Weight gain was normal, and no animal deaths were observed (see table below). LC50 2h > 10185.19 mg / m³ 3 At the end of the experiment, all animals underwent gross dissection, and no abnormalities were observed by visual inspection.

[0033] Table of acute inhalation toxicity test results (IV) Conclusion The test substance was tested according to the acute inhalation toxicity test in section 2.3.2 of the "Disinfection Technical Specifications" (2002 edition). Under the conditions of this test, the acute inhalation toxicity LC5002h in KM mice was >10185.19 mg / m³. 3 The toxicity classification indicates it is practically non-toxic.

[0034] Example 6: Skin Allergy Test 1. Test sample: contents of the antibacterial module.

[0035] 2. Laboratory animals and their housing conditions: Experimental animals: 32 conventional-grade Hartley guinea pigs, half male and half female, with body weight of 200g~300g, provided by Guangzhou Tianzhu Biotechnology Co., Ltd. The production license number of experimental animals is SCXK (Guangdong) 2024-0071, and the quality certificate number is 44838300000663. The animals were quarantined in the laboratory animal room environment for 6 days before the test.

[0036] Breeding conditions: The feed was provided by Guangzhou Innove Nutrition Feed Co., Ltd., with production license No. Yue Feed Certificate (2024) 01154, and quality certificate No. 202504030036. The temperature of the animal room is 18°C~29°C, and the relative humidity is 40%~70%, with 12h of light and 12h of darkness.

[0037] (II) Methods 1. Test basis: Skin allergy test specified in Section 2.3.6 of *Disinfection Technical Specifications* (2002 Edition). 2. Preparation of test substance: With reference to GB / T16886.12-2017, 24.6 g of the module was added into 123 mL of sterile water at a ratio of 0.2 g / mL, and extracted at 37°C for 72 h. The extract was taken as the test solution. Preliminary test results showed that the original test solution caused no skin irritation reaction to the test animals, therefore the original test solution was used for both induction and challenge in the test group during the test; the original test solution was used for challenge in the negative control group; 1% 2,4-dinitrochlorobenzene solution was used for induction, and 0.5% 2,4-dinitrochlorobenzene solution was used for challenge in the positive control group (test period of the positive control group: from February 12, 2025 to March 15, 2025, once every six months).

[0038] 3. Test grouping: Guinea pigs were randomly divided into a test group and a negative control group, with half male and half female in each group.

[0039] 4. Exposure method 4.1 Induction: 24 hours before the test, the hair on the left back of the animal within a 3cm×3cm area was removed. For the test group, 0.5 mL of the test substance at induction concentration was directly applied to the 2cm×2cm depilated skin on the left side, covered with two layers of gauze and one layer of non-irritating plastic film, then fixed with non-irritating adhesive tape, and maintained for 6 hours. The procedure was repeated once on the 7th day and the 14th day. The 1% 2,4-dinitrochlorobenzene solution in the positive control group was treated in the same way. No treatment was performed for the negative control.

[0040] 4.2 Challenge: On the 14th day after the last induction, 0.5 mL of the test substance at challenge concentration was directly applied to the 2cm×2cm depilated skin on the right side of animals in the test group and the negative control group, covered with two layers of gauze and one layer of non-irritating plastic film, then fixed with non-irritating adhesive tape. The residual test substance was washed away after 6 hours. The 0.5% 2,4-dinitrochlorobenzene solution in the positive control group was treated in the same way.

[0041] 5. Evaluation indicators: Skin reactions were observed and scored 24h and 48h after stimulation treatment to assess the sensitization intensity. The scoring criteria were based on Tables 2-18 and 2-19 of "Skin Allergy Test" in Section 2.3.6 of the "Disinfection Technical Specifications" (2002 edition).

[0042] (III) Test Results The skin reaction scores at the test sites were observed 24 h and 48 h after the test substance was activated.

[0043] Results of skin allergic reactions in guinea pigs Note: The results of skin erythema and edema reaction intensity refer to the number of animals when the skin reaction score is 0, 1, 2, 3, or 4.

[0044] (IV) Conclusion The contents of the antibacterial module were tested according to GB / T16886.12-2017. 24.6g of the module was added to 123 mL of sterile water at a ratio of 0.2g / mL and extracted at 37℃ for 72h. The extract was used as the test solution and tested according to the skin allergy test 2.3.6 of the "Disinfection Technical Specifications" (2002 edition). Under the test conditions, no skin allergy was observed in Hartley guinea pigs.

[0045] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A high flash point mustard oil, characterized in that, It is composed of the following components in parts by weight: isopropyl myristate: 7-13 parts, mustard oil: 78-82 parts, and triethyl citrate: 7-13 parts.

2. The high flash point mustard oil according to claim 1, characterized in that, It consists of the following components in parts by weight: isopropyl myristate: 7 parts, mustard oil: 78 parts, and triethyl citrate: 7 parts.

3. The high flash point mustard oil according to claim 1, characterized in that, It consists of the following components in parts by weight: isopropyl myristate: 10 parts, mustard oil: 80 parts, and triethyl citrate: 10 parts.

4. The high flash point mustard oil according to claim 1, characterized in that, It is composed of the following components in parts by weight: isopropyl myristate: 13 parts, mustard oil: 82 parts, and triethyl citrate: 13 parts.

5. A method for preparing high flash point mustard oil, characterized in that, Includes the following steps: (S1) According to the weight ratio, add isopropyl myristate and triethyl citrate into the reaction vessel, and stir evenly for more than 30 minutes at room temperature of 25°C and stirring speed of 280-320 rpm to obtain the mixed excipient liquid. (S2) Add the corresponding weight of mustard oil to the mixed excipient liquid obtained in step (S1), and stir continuously at a stirring speed of 280-320 rpm for 60 minutes until the material is evenly mixed. (S3) After stirring evenly, let the mixture stand for 24 hours to mature, and finally bottle it to obtain high flash point mustard oil.

6. The method for preparing high flash point mustard oil according to claim 5, characterized in that, The high flash point mustard oil in step (S3) has a flash point of 62°C and has a broad-spectrum antibacterial effect. It can inhibit the growth and reproduction of bacteria and mold in the space through its own volatility and can achieve antibacterial effect without direct contact with objects.

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