A composition with the effect of regulating intestinal flora and application

Abstract

The application belongs to the technical field of food, and particularly discloses a composition with a function of regulating intestinal flora and application and a preparation method of the composition with the function of regulating intestinal flora. The application first uniformly mixes gardenia fruit powder, chickpea powder, spearmint powder and radix sophorae tonkinensis powder, and then inoculates and ferments Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid to obtain the composition with the function of regulating intestinal flora, which can effectively increase the number of beneficial bacteria, inhibit harmful bacteria, and has a good improvement effect on colitis.

Description

Technical Field

[0001] This invention relates to the field of food technology, and more specifically to a composition and its application that regulates intestinal flora. Background Technology

[0002] The gut is the largest micro-ecosystem in the human body, and the homeostasis of the gut microbiota is the core foundation for maintaining the integrity of the intestinal barrier and the normal functioning of the body's immune metabolism. Normal gut microbiota can maintain intestinal physiological function through multiple pathways, such as participating in nutrient metabolism, synthesizing short-chain fatty acids, inhibiting the colonization of pathogenic bacteria, and regulating intestinal inflammatory responses.

[0003] When external stimuli, dietary imbalances, or abnormal metabolism occur, intestinal flora imbalance can be triggered, manifesting as a significant decrease in the abundance of beneficial bacteria such as lactic acid bacteria and an abnormal proliferation of harmful bacteria such as Clostridium perfringens. This can damage the intestinal mucosal barrier, induce chronic low-grade intestinal inflammation, and in severe cases, gradually develop into inflammatory bowel diseases such as ulcerative colitis and Crohn's disease, resulting in typical pathological symptoms such as colonic atrophy and shortening, diarrhea, and intestinal mucosal erosion and damage, seriously affecting the body's health and quality of life.

[0004] Currently, interventions for gut microbiota imbalance and colitis mainly focus on chemical drug treatment, probiotic supplementation, and dietary fiber intervention. While chemical anti-inflammatory drugs can quickly relieve intestinal inflammation, they have drawbacks such as limited target action, potential for drug resistance with long-term use, damage to normal metabolism, and inability to fundamentally repair gut microbiota imbalance. Probiotic supplementation is easily affected by gastric acid, bile, and the existing gut microenvironment, resulting in low colonization rates, unstable effects, and difficulty in maintaining long-term microbiota balance. Ordinary dietary fiber preparations only provide basic nutrition to the gut microbiota and lack anti-inflammatory and intestinal mucosal repair effects, showing poor repair effects on existing intestinal inflammatory damage and colonic pathological damage.

[0005] Therefore, this application is submitted. Summary of the Invention

[0006] This invention provides a composition and its application that regulates intestinal flora. The composition described in this application has excellent effects in regulating intestinal flora, effectively increasing the number of beneficial bacteria and inhibiting harmful bacteria, while also having a good effect on improving colitis.

[0007] The present invention solves its technical problem by adopting the following technical solution: A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder evenly to obtain a mixed powder; (2) Inoculate the Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid into the mixed powder, ferment, sterilize, freeze dry, and obtain a composition that has the function of regulating intestinal flora.

[0008] As an implementation scheme of this application, the characteristic is that the mass ratio of gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder is (25~32):(22~30):(21~28):(17~25).

[0009] As an embodiment of this application, the mass ratio of the Lactobacillus paracasei seed liquid, Lactobacillus johnsonii seed liquid, and mixed powder is (0.8~1.5):(0.8~1.5):100.

[0010] As an embodiment of this application, the fermentation temperature is 35~38℃ and the fermentation time is 24~72h.

[0011] As an embodiment of this application, the method for preparing the Lactobacillus paracasei seed liquid is as follows: Lactobacillus paracasei is inoculated into MRS medium and fermented at 34~37℃ for 24~48h to obtain Lactobacillus paracasei seed liquid.

[0012] As an embodiment of this application, the inoculation amount of Lactobacillus paracasei is 1~4 wt%.

[0013] As an embodiment of this application, the method for preparing the Lactobacillus johnsonii seed liquid is as follows: Lactobacillus johnsonii is inoculated into MRS medium and fermented at 34~37℃ for 24~48h to obtain Lactobacillus johnsonii seed liquid.

[0014] As an embodiment of this application, the inoculation amount of Lactobacillus johnsonii is 1~4wt%.

[0015] This application also provides a composition that regulates intestinal flora, prepared by the preparation method described above.

[0016] This application also provides the use of a composition having the effect of regulating intestinal flora in the preparation of food or pharmaceuticals having the effect of regulating intestinal flora.

[0017] The beneficial effects of the present invention are as follows: In this application, gardenia fruit powder, chickpea powder, spearmint powder, and light soybean powder are mixed evenly, and then Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid are inoculated and fermented to obtain a composition that has the effect of regulating intestinal flora. The composition can effectively increase the number of beneficial bacteria and inhibit harmful bacteria, and at the same time has a good improvement effect on colitis. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0020] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0021] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0022] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0023] The Lactobacillus paracasei mentioned in this application is Lactobacillus paracasei ATCC 334, which is derived from the China Industrial Microbial Culture Collection Center.

[0024] The Lactobacillus johnsonii mentioned in this application is Lactobacillus johnsonii CICC 6084, which is derived from the China Industrial Microbial Culture Collection Center.

[0025] The MRS culture medium mentioned in this application is derived from Huankai Microbiology.

[0026] This application provides a method for preparing a composition that regulates intestinal flora, comprising the following steps: (1) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder evenly to obtain a mixed powder; (2) Inoculate the Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid into the mixed powder, ferment, sterilize, freeze dry, and obtain a composition that has the function of regulating intestinal flora.

[0027] This application first mixes gardenia fruit powder, chickpea powder, spearmint powder, and light soy sauce powder evenly, and then inoculates and ferments Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid to obtain a composition that has the effect of regulating intestinal flora. The composition can effectively increase the number of beneficial bacteria and inhibit harmful bacteria, and at the same time has a good effect on improving colitis.

[0028] Among them, gardenia fruit can effectively inhibit intestinal inflammatory response, repair colonic mucosal damage, improve colitis pathological damage, and regulate intestinal microecology; chickpeas are rich in high-quality dietary fiber, which can act as a prebiotic to promote the proliferation of beneficial intestinal bacteria and lactic acid bacteria, increase the production of short-chain fatty acids, and maintain normal colonic growth and intestinal barrier function; spearmint can significantly inhibit the excessive proliferation of harmful bacteria such as Clostridium perfringens and regulate the balance of intestinal microecology; and fermented soybeans are made through microbial fermentation and contain rich active small molecules and beneficial bacteria, which can further stabilize the colonization of beneficial bacteria in the intestine. In total, gardenia fruit, chickpeas, spearmint, and fermented soybeans, through multiple synergistic effects of anti-inflammatory mucosal repair, promoting beneficial bacteria growth, inhibiting harmful bacteria, and stabilizing intestinal microecology, effectively reverse DSS-induced colonic shortening, reduce intestinal inflammation levels, significantly improve intestinal flora imbalance, exert good protective and repairing effects against colitis, and effectively increase the number of beneficial bacteria and inhibit harmful bacteria.

[0029] In some embodiments, the gardenia fruit powder, chickpea powder, spearmint powder, and light soy sauce powder are characterized by a mass ratio of (25~32):(22~30):(21~28):(17~25).

[0030] In some embodiments, the mass ratio of the Lactobacillus paracasei seed liquid, Lactobacillus johnsonii seed liquid, and mixed powder is (0.8~1.5):(0.8~1.5):100.

[0031] In some embodiments, the fermentation temperature is 35~38°C and the fermentation time is 24~72h.

[0032] In some embodiments, the method for preparing the Lactobacillus paracasei seed culture is as follows: Lactobacillus paracasei is inoculated into MRS medium and fermented at 34-37°C for 24-48 hours to obtain Lactobacillus paracasei seed culture.

[0033] In some embodiments, the inoculum amount of Lactobacillus paracasei is 1-4 wt%.

[0034] In some embodiments, the method for preparing the Lactobacillus johnsonii seed culture is as follows: Lactobacillus johnsonii is inoculated into MRS medium and fermented at 34-37°C for 24-48 hours to obtain Lactobacillus johnsonii seed culture.

[0035] In some embodiments, the inoculum amount of Lactobacillus johnsonii is 1-4 wt%.

[0036] In some embodiments, the particle size of the gardenia fruit powder is 50-500 mesh.

[0037] In some embodiments, the chickpea flour has a particle size of 50 to 500 mesh.

[0038] In some embodiments, the spearmint powder has a particle size of 50-500 mesh.

[0039] In some embodiments, the particle size of the light fermented soybean powder is 50-500 mesh.

[0040] One embodiment of this application provides a composition that regulates intestinal flora, prepared using the preparation method described above.

[0041] One embodiment of this application provides the use of a composition having the effect of regulating intestinal flora in the preparation of food or medicine having the effect of regulating intestinal flora.

[0042] The present application is further illustrated below with specific embodiments:

[0043] Example 1 A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0044] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0045] (2) Wash and dry gardenia fruit, chickpea, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then grind gardenia fruit, chickpea, spearmint and light soybean into 200 mesh respectively to obtain gardenia fruit powder, chickpea powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder in a mass ratio of 30:26:24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0046] Example 2 A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0047] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0048] (2) Wash and dry gardenia fruit, chickpea, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then grind gardenia fruit, chickpea, spearmint and light soybean into 200 mesh respectively to obtain gardenia fruit powder, chickpea powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder, spearmint powder and light fermented soybean powder in a mass ratio of 32:30:21:17 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1 wt% of Lactobacillus paracasei seed liquid and 1 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition that has the function of regulating intestinal flora.

[0049] Example 3 A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0050] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0051] (2) Wash and dry gardenia fruit, chickpea, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then grind gardenia fruit, chickpea, spearmint and light soybean into 200 mesh respectively to obtain gardenia fruit powder, chickpea powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder in a mass ratio of 25:22:28:25 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.5 wt% of Lactobacillus paracasei seed liquid and 1.5 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0052] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the mass ratios of gardenia fruit powder, chickpea powder, spearmint powder, and light soy sauce powder are different, while all other ratios are the same.

[0053] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0054] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0055] (2) Wash and dry gardenia fruit, chickpea, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then grind gardenia fruit, chickpea, spearmint and light soybean into 200 mesh respectively to obtain gardenia fruit powder, chickpea powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder in a mass ratio of 15:15:35:35 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0056] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the mass ratios of gardenia fruit powder, chickpea powder, spearmint powder, and light soy sauce powder are different, while all other ratios are the same.

[0057] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0058] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0059] (2) Wash and dry gardenia fruit, chickpea, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then grind gardenia fruit, chickpea, spearmint and light soybean into 200 mesh respectively to obtain gardenia fruit powder, chickpea powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder in a mass ratio of 40:35:15:10 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0060] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain gardenia fruit powder, but everything else is the same.

[0061] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0062] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0063] (2) Wash and dry chickpeas, spearmint and light soybeans, sterilize at 85℃ for 30 minutes, then grind chickpeas, spearmint and light soybeans to 200 mesh to obtain chickpea powder, spearmint powder and light soybean powder. (3) Mix chickpea flour, spearmint powder, and light soy sauce powder in a mass ratio of 26:24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0064] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain chickpea flour, but everything else is the same.

[0065] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0066] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0067] (2) Wash and dry gardenia fruit, spearmint and light soybean, sterilize at 85℃ for 30 minutes, then pulverize gardenia fruit, spearmint and light soybean to 200 mesh to obtain gardenia fruit powder, spearmint powder and light soybean powder. (3) Mix gardenia fruit powder, spearmint powder and light fermented soybean powder in a mass ratio of 30:24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0068] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain spearmint powder, but everything else is the same.

[0069] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0070] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0071] (2) Wash and dry gardenia fruit, chickpeas and light soybeans, sterilize at 85℃ for 30 minutes, then pulverize gardenia fruit, chickpeas and light soybeans to 200 mesh to obtain gardenia fruit powder, chickpea powder and light soybean powder. (3) Mix gardenia fruit powder, chickpea powder and light soybean powder in a mass ratio of 30:26:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0072] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain light fermented soybean powder, but all other aspects are the same.

[0073] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0074] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0075] (2) Wash and dry gardenia fruit, chickpeas and spearmint, sterilize at 85℃ for 30 minutes, then pulverize gardenia fruit, chickpeas and spearmint to 200 mesh to obtain gardenia fruit powder, chickpea powder and spearmint powder. (3) Mix gardenia fruit powder, chickpea powder and spearmint powder in a mass ratio of 30:26:24 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0076] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 does not contain gardenia fruit powder or chickpea powder, but all other aspects are the same.

[0077] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0078] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0079] (2) Wash and dry the spearmint and light soybeans, sterilize them at 85°C for 30 minutes, and then grind the spearmint and light soybeans to 200 mesh to obtain spearmint powder and light soybean powder. (3) Mix spearmint powder and light soy sauce powder at a mass ratio of 24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0080] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 does not contain spearmint powder or light soy sauce powder, but all other aspects are the same.

[0081] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0082] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0083] (2) Wash and dry the gardenia fruit and chickpeas, sterilize them at 85℃ for 30 minutes, and then grind the gardenia fruit and chickpeas to 200 mesh to obtain gardenia fruit powder and chickpea powder. (3) Mix gardenia fruit powder and chickpea powder evenly at a mass ratio of 30:26 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0084] Comparative Example 9 The difference between Comparative Example 9 and Example 1 is that Comparative Example 9 does not contain chickpea flour or spearmint powder, but all other aspects are the same.

[0085] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0086] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0087] (2) Wash and dry the gardenia fruit and light soybeans, sterilize them at 85℃ for 30 minutes, and then pulverize the gardenia fruit and light soybeans to 200 mesh to obtain gardenia fruit powder and light soybean powder. (3) Mix gardenia fruit powder and light soybean powder at a mass ratio of 26:24 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0088] Comparative Example 10 The difference between Comparative Example 10 and Example 1 is that Comparative Example 10 uses an equal amount of dried tangerine peel powder to replace gardenia fruit powder and an equal amount of cinnamon powder to replace chickpea powder; all other aspects are the same.

[0089] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0090] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0091] (2) Wash and dry the dried tangerine peel, cinnamon, spearmint and light fermented soybean, sterilize at 85℃ for 30 minutes, and then grind the dried tangerine peel, cinnamon, spearmint and light fermented soybean into 200 mesh to obtain dried tangerine peel powder, cinnamon powder, spearmint powder and light fermented soybean powder. (3) Mix the dried tangerine peel powder, cinnamon powder, spearmint powder and light fermented black bean powder evenly in a mass ratio of 30:26:24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0092] Comparative Example 11 The difference between Comparative Example 11 and Example 1 is that Comparative Example 11 uses an equal amount of perilla powder to replace spearmint powder and an equal amount of roasted licorice powder to replace light soy sauce powder; all other aspects are the same.

[0093] A method for preparing a composition having the effect of regulating intestinal flora includes the following steps: (1) Inoculate Lactobacillus paracasei into MRS medium at an inoculation rate of 2 wt%, and ferment at 36°C for 36 h to obtain Lactobacillus paracasei seed liquid for later use.

[0094] Lactobacillus johnsonii was inoculated into MRS medium at an inoculum of 2 wt% and fermented at 36°C for 36 h to obtain Lactobacillus johnsonii seed culture for later use.

[0095] (2) Wash and dry gardenia fruit, chickpea, perilla and roasted licorice, sterilize at 85℃ for 30 minutes, then pulverize gardenia fruit, chickpea, perilla and roasted licorice to 200 mesh to obtain gardenia fruit powder, chickpea powder, perilla powder and roasted licorice powder. (3) Mix gardenia fruit powder, chickpea powder, perilla powder and roasted licorice powder in a mass ratio of 30:26:24:20 to obtain a mixed powder; (4) Add the mixed powder to the fermenter, and then inoculate the mixed powder with 1.2 wt% of Lactobacillus paracasei seed liquid and 1.2 wt% of Lactobacillus johnsonii seed liquid. Ferment at 36°C for 72 h, sterilize at 75°C for 30 s, freeze dry, and obtain a composition with the function of regulating intestinal flora.

[0096] Test case 1. Toxicity test: Fifty-six SD rats weighing 250±5g were selected, with half males and half females, and randomly divided into 14 groups of 4 rats each. The rats were administered 0.8g·kg by gavage three times (once a day). -1 ·d -1 The patients were fasted for 16 hours before gavage and observed for two weeks afterward. Symptoms of poisoning and mortality were recorded, as shown in Table 1. During the gavage test, the patients were fed with standard feed as usual.

[0097] Table 1

[0098] As can be seen from Table 1, the composition described in this application has good safety.

[0099] 2. Efficacy Experiment: 2.1 Eighty C57BL / 6 mice weighing 20±0.5g were randomly divided into 16 groups of 5 mice each: 14 experimental groups, 1 model group, and 1 blank control group.

[0100] 2.2 After feeding mice normally for 5 days, feces were aseptically collected from the anus of mice to count the number of beneficial bacteria (represented by lactic acid bacteria) and harmful bacteria (Clostridium perfringens).

[0101] 2.3 Except for the blank control group, mice in other groups were given free access to 2.5% DSS aqueous solution for 7 consecutive days; subsequently, they were given sterile water and administered 0.8 g / kg via gavage once daily for 7 consecutive days. -1 ·d -1 The compositions of the examples and comparative examples, the blank control group and the model group were administered an equal volume of sterile water by gavage.

[0102] 2.4 Twelve hours after the last administration, the mice were anesthetized and then sacrificed. The colon and feces from the anus of the mice were collected aseptically. The length of the colon was counted, and the number of beneficial bacteria (represented by lactic acid bacteria) and harmful bacteria (Clostridium perfringens) were counted.

[0103] Calculate the change rate of lactic acid bacteria = 100% (Number of lactic acid bacteria after the experiment - number of lactic acid bacteria before the experiment) / number of lactic acid bacteria before the experiment.

[0104] Calculate the change rate of Clostridium perfringens = 100% (Number of Clostridium perfringens after the test - Number of Clostridium perfringens before the test) / Number of Clostridium perfringens before the test

[0105] Table 2

[0106] As can be seen from Table 2, the composition described in this application has excellent effects in regulating intestinal flora, effectively increasing the number of beneficial bacteria and inhibiting harmful bacteria, while also having a good effect in improving colitis.

[0107] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a composition having an effect of regulating intestinal flora, characterized by, Includes the following steps: (1) Mix gardenia fruit powder, chickpea powder, spearmint powder and light soy sauce powder evenly to obtain a mixed powder; (2) Inoculate the Lactobacillus paracasei seed liquid and Lactobacillus johnsonii seed liquid into the mixed powder, ferment, sterilize, freeze dry, and obtain a composition that has the function of regulating intestinal flora.

2. The method for preparing a composition having an effect of adjusting intestinal flora according to claim 1, characterized in that, The mass ratio of gardenia fruit powder, chickpea powder, spearmint powder, and light soy sauce powder is (25~32):(22~30):(21~28):(17~25).

3. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The mass ratio of Lactobacillus paracasei seed liquid, Lactobacillus johnsonii seed liquid, and mixed powder is (0.8~1.5):(0.8~1.5):

100.

4. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The fermentation temperature is 35~38℃, and the fermentation time is 24~72h.

5. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The method for preparing the Lactobacillus paracasei seed culture is as follows: Lactobacillus paracasei is inoculated into MRS medium and fermented at 34-37℃ for 24-48 hours to obtain Lactobacillus paracasei seed culture.

6. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The inoculation amount of Lactobacillus paracasei is 1-4 wt%.

7. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The method for preparing the Lactobacillus johnsonii seed liquid is as follows: Lactobacillus johnsonii is inoculated into MRS medium and fermented at 34~37℃ for 24~48h to obtain Lactobacillus johnsonii seed liquid.

8. The method for preparing the composition with the function of regulating intestinal flora according to claim 1, characterized in that, The inoculation amount of Lactobacillus johnsonii is 1-4 wt%.

9. A composition having the effect of regulating intestinal flora, characterized in that, It is prepared by any of the preparation methods described in claims 1 to 8.

10. The use of the composition of claim 9, which has the effect of regulating intestinal flora, in the preparation of food or medicine having the effect of regulating intestinal flora.

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