An antioxidant composition and its use in the preparation of antioxidant tea drinks, medicaments or health products

Abstract

The application provides an antioxidant composition and application thereof in preparation of antioxidant tea drinks, medicines or health products, and belongs to the technical field of flower and fruit tea compositions. Peach blossoms, rose flowers, Kunlun snow chrysanthemums, osmanthus flowers, jasmine flowers and Chinese allspice flowers are combined, and through metabolomics combined with antioxidant activity evaluation technology, it is determined that the tea drink obtained after the combination of the six components has a unique metabolite spectrum that is different from other combinations, indicating that the combination of the components forms a new synergistic material basis, and the antioxidant activity of the combination of the six components is significantly higher than that of other combinations, and the effect exceeds the simple addition of each single medicinal material, confirming that the components in the prescription have a synergistic antioxidant effect.

Description

Technical Field

[0001] This invention relates to the field of herbal tea composition processing technology, specifically to an antioxidant composition and its application in the preparation of antioxidant tea drinks, medicines or health products. Background Technology

[0002] Herbal teas are beverages similar to tea made from non-tea plant materials such as flowers, leaves, and fruits. In recent years, with consumers paying more attention to the "natural, healthy, and functional" qualities of food ingredients, plant-based ingredients have become a hot topic in the development of functional foods.

[0003] While various herbal tea products are currently available on the market, most are simple blends based on traditional experience, lacking systematic identification of functional components. Modern research indicates that oxidative stress is a significant factor contributing to aging, dull skin, and various metabolic disorders. Therefore, developing natural compound compositions with good antioxidant activity is of great value. Summary of the Invention

[0004] In view of this, the object of the present invention is to provide an antioxidant composition and its application in the preparation of antioxidant teas, pharmaceuticals, or health products. The antioxidant composition provided by the present invention, when formulated, can enrich the metabolic spectrum and has a good synergistic antioxidant effect.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides an antioxidant composition comprising the following components in parts by weight: Peach blossoms: 0.5-1 part; 1-2 portions of roses; Kunlun snow chrysanthemum 0.5~1 part; Osmanthus flowers 0.5-1 part; Jasmine flowers, 0.5-1.5 parts; 0.5 to 1.5 parts of mimosa flowers.

[0006] Preferably, the components include the following parts by weight: 0.5 portions of peach blossoms; One serving of roses; Kunlun Snow Chrysanthemum 0.5 parts; Osmanthus flowers, 0.6 parts; One serving of jasmine flowers; One portion of mimosa flowers.

[0007] Preferably, the rose is a double-petaled red rose.

[0008] Preferably, the moisture content of the peach blossoms, roses, Kunlun snow chrysanthemums, osmanthus, jasmine, or mimosa flowers is ≤9%.

[0009] This invention provides the application of the above-mentioned antioxidant composition in the preparation of antioxidant teas, antioxidant drugs or antioxidant health products.

[0010] This invention provides an antioxidant tea beverage, which is obtained by brewing the above-mentioned antioxidant composition.

[0011] Preferably, the water used for brewing is boiling water, and the brewing time is 20-40 minutes.

[0012] This invention provides an antioxidant composition comprising the following components in parts by weight: 0.5-1 part peach blossom; 1-2 parts rose; 0.5-1 part Kunlun snow chrysanthemum; 0.5-1 part osmanthus; 0.5-1.5 parts jasmine; and 0.5-1.5 parts mimosa. According to traditional Chinese medicine theory, rose has the effects of regulating qi and relieving depression, promoting blood circulation and relieving pain; peach blossom has the effects of promoting blood circulation and relieving constipation; Kunlun snow chrysanthemum has the effects of clearing heat and detoxifying, promoting blood circulation and removing blood stasis; osmanthus has the effects of warming the lungs and resolving phlegm, dispelling cold and relieving pain; jasmine has the effects of regulating qi and relieving pain, dispelling foulness and relieving depression; mimosa has the effects of relieving depression and calming the mind; and wolfberry has the effects of nourishing the liver and kidneys, benefiting essence and improving eyesight. This invention combines peach blossom, rose, Kunlun snow chrysanthemum, osmanthus, jasmine, and mimosa flowers. Through metabolomics combined with antioxidant activity evaluation technology, it was determined that the tea obtained after combining these six ingredients produced a unique metabolic profile that is different from other formulations. This indicates that the combination forms a new synergistic material basis, and the antioxidant activity of this combination is significantly higher than that of other formulations. Its effect exceeds the simple sum of the individual herbs, confirming that there is a synergistic antioxidant effect among the components in the formula. The results of the examples show that the total antioxidant capacity of the tea infusion formulation three (peach blossom, rose, Kunlun snow chrysanthemum, osmanthus, jasmine, and mimosa) of the present invention is 112.55 μmol / mL, which is significantly higher than that of formulation one (peach blossom, rose, and Kunlun snow chrysanthemum) and formulation two (peach blossom, rose, Kunlun snow chrysanthemum, and wolfberry). Principal component analysis (PCA) of metabolites shows that, compared with formulation one and formulation two, the antioxidant composition formulation three of the present invention contains less trans-ferulic acid, N-acetyl-L-glutamic acid, 3-isopropylmalic acid, and 5- O The significantly increased content of feruloylquinic acid indicates that the antioxidant composition of the present invention has stronger anti-inflammatory, antioxidant, and energy metabolism regulation capabilities. Attached Figure Description

[0013] Figure 1 Principal component analysis (PCA) of metabolites from the three tea formulas is shown. Figure 2 A comparison chart of the total antioxidant capacity of ABTS in the three tea formulas. Detailed Implementation

[0014] This invention provides an antioxidant composition comprising the following components in parts by weight: Peach blossoms: 0.5-1 part; 1-2 portions of roses; Kunlun snow chrysanthemum 0.5~1 part; Osmanthus flowers 0.5-1 part; Jasmine flowers, 0.5-1.5 parts; 0.5 to 1.5 parts of mimosa flowers.

[0015] The antioxidant composition of the present invention comprises 0.5 to 1 part of peach blossom by weight percentage, specifically 0.5, 0.6, 0.7, 0.8, 0.9 or 1 part.

[0016] Based on the mass fraction of the peach blossoms, the antioxidant composition of the present invention includes 1 to 2 parts of rose petals, specifically 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, or 2 parts. In the present invention, the rose petals are preferably double-petaled red roses.

[0017] Based on the mass fraction of the peach blossoms, the antioxidant composition of the present invention includes 0.5 to 1 part of Kunlun snow chrysanthemum, specifically 0.5, 0.6, 0.7, 0.8, 0.9 or 1 part.

[0018] Based on the mass fraction of the peach blossoms, the antioxidant composition of the present invention includes 0.5 to 1 part of osmanthus, specifically 0.5, 0.6, 0.7, 0.8, 0.9 or 1 part.

[0019] Based on the mass fraction of the peach blossoms, the antioxidant composition of the present invention includes 0.5 to 1.5 parts of jasmine flowers, specifically 0.5, 0.6, 0.8, 1, 1.2, 1.4, or 1.5 parts.

[0020] Based on the mass fraction of the peach blossoms, the antioxidant composition of the present invention includes 0.5 to 1.5 parts of mimosa flowers, specifically 0.5, 0.6, 0.8, 1, 1.2, 1.4, or 1.5 parts.

[0021] In this invention, the moisture content of the peach blossoms, roses, Kunlun snow chrysanthemums, osmanthus, jasmine, or mimosa flowers is preferably ≤9%, more preferably 5~8%.

[0022] As a specific embodiment of the present invention, the antioxidant composition preferably comprises the following components in parts by weight: 0.5 portions of peach blossoms; One serving of roses; Kunlun Snow Chrysanthemum 0.5 parts; Osmanthus flowers, 0.6 parts; One serving of jasmine flowers; One portion of mimosa flowers.

[0023] This invention provides the application of the above-mentioned antioxidant composition in the preparation of antioxidant teas, antioxidant drugs or antioxidant health products.

[0024] This invention provides an antioxidant tea beverage, obtained by brewing the above-mentioned antioxidant composition. In this invention, the water used for brewing is preferably boiling water, and the brewing time is preferably 20-40 minutes, more preferably 30 minutes.

[0025] The antioxidant tea of ​​this invention contains the following main active ingredients: D-(+)-raffinose, 6-phosphogluconic acid, galactitol, (-)-quinic acid, chlorogenic acid, citric acid, 3-isopropylmalic acid, D-gluconic acid, gallic acid, trans-ferric acid, N-acetyl-L-glutamic acid, and 5- O - One or more of feruloylquinic acid.

[0026] The following examples illustrate the antioxidant compositions provided by the present invention and their application in the preparation of antioxidant teas, pharmaceuticals, or health products. However, these examples should not be construed as limiting the scope of protection of the present invention.

[0027] Example The raw materials selected are dried, impurity-free authentic medicinal herbs: peach blossoms, roses (double-petaled red roses), Kunlun snow chrysanthemums, wolfberries, osmanthus, jasmine, and mimosa flowers.

[0028] Three formulas are set up, wherein formula one is: peach blossom, rose, and Kunlun snow chrysanthemum; Formula 2 consists of: peach blossom, rose, Kunlun snow chrysanthemum, and wolfberry; Formula 3 consists of: peach blossom, rose, Kunlun snow chrysanthemum, osmanthus, jasmine, and mimosa.

[0029] The proportions of raw materials for each component are shown in Table 1: Table 1. Raw material ratios for each component

[0030] The preparation process for each tea formula is as follows: Place each of the weighed ingredients into a 250 mL beaker, add 100 mL of boiling water (100℃), cover and soak for 30 min. After cooling to room temperature, use as the test solution.

[0031] Performance Test 1: Metabolite Detection Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF MS) was used to detect the tea infusions in each group.

[0032] (1) Chromatographic conditions Samples were separated using an Agilent 1290 Infinity LC ultra-high performance liquid chromatography (UHPLC) system with a HILIC column; column temperature 25℃; flow rate 0.5 mL / min; injection volume 2 μL; mobile phase composition A: water + 25 mM ammonium acetate + 25 mM ammonia, B: acetonitrile; gradient elution program as follows: 0–0.5 min, 95%; 0.5–7 min, B linearly changes from 95% to 65%; 7–8 min, B linearly changes from 65% to 40%; 8–9 min, B maintains at 40%; 9–9.1 min, B linearly changes from 40% to 95%; 9.1–12 min, B maintains at 95%; throughout the analysis, samples were placed in an autosampler at 4℃. To avoid the influence of instrument signal fluctuations, samples were analyzed sequentially in a random order. QC samples were inserted into the sample queue to monitor and evaluate the stability of the system and the reliability of the experimental data.

[0033] (2) Q-TOF mass spectrometry conditions The first and second-order spectra of the samples were acquired using an AB Triple TOF 6600 mass spectrometer.

[0034] After separation using an Agilent 1290 Infinity LC ultra-high performance liquid chromatography (UHPLC) system, the samples were analyzed by mass spectrometry using a Triple TOF 6600 mass spectrometer (AB SCIEX), with detection performed in both positive and negative electrospray ionization (ESI) modes.

[0035] The ESI source settings are as follows: Auxiliary heating gas 1 (Gas1): 60, Auxiliary heating gas 2 (Gas2): 60, Curtain gas (CUR): 30psi, Ion source temperature: 600℃, Spray voltage (ISVF): ±5500 V (positive and negative modes); Primary mass-to-charge ratio detection range: 60-1000 Da, Secondary fragment ion mass-to-charge ratio detection range: 25~1000 Da, Primary mass spectrometry scan cumulative time: 0.20 s / spectra, Secondary mass spectrometry scan cumulative time: 0.05 s / spectra; Secondary mass spectrometry is obtained using data-dependent acquisition mode (IDA) and peak intensity value screening mode, Declustering voltage (DP): ±60 V (positive and negative modes), Collision energy: 35±15 eV, IDA settings are as follows: Dynamic exclusion range of isotopic ions: 4 Da, 10 fragment spectra are acquired per scan.

[0036] The collected raw data were imported into metabolomics processing software for peak alignment, extraction, and normalization. Principal component analysis (PCA) was used to compare the differences in metabolite profiles among the three groups of samples. The main active substances of formulation 1 are shown in Table 2, the main active substances of formulation 2 with increased content compared to formulation 1 are shown in Table 3, and the main active substances of formulation 3 with increased content compared to formulations 1 and 2 are shown in Table 4.

[0037] Table 2. Main active ingredients of Formula 1 (characteristics of the basic formula)

[0038] Table 3. Increase in the content of main active substances in Formulation 2 compared to Formulation 1

[0039] Table 4. Increase in main active substances in Formulation 3 compared to Formulations 1 and 2

[0040] As can be seen from Tables 2-4, the main active ingredient of Formula 1 is concentrated in carbohydrate metabolism, indicating that Formula 1 may be more inclined to be a basal metabolic maintenance formula.

[0041] Compared with Formulation 1, the contents of (-)-quinic acid, chlorogenic acid, citric acid, 3-isopropylmalic acid, D-gluconic acid and gallic acid in Formulation 2 were significantly increased.

[0042] Compared to Formula 1, Formula 3 significantly increases the amount of amino acid derivatives, phenolic acids, and organic acids, indicating that Formula 3 places greater emphasis on the synergistic effect of amino acid metabolism and antioxidant activity. The unique advantage of Formula 3 lies in its organic acid metabolites (such as 3-isopropylmalic acid) and highly active phenolic acids (ferulic acid, 5-...). O The significant enrichment of feruloylquinic acid may endow Formulation 3 with stronger anti-inflammatory, antioxidant, and energy metabolism regulation capabilities.

[0043] Principal component analysis (PCA) diagrams of metabolites from the three tea formulas are shown below. Figure 1 As shown. Figure 1 The sample points of Formula 1 and Formula 2 are quite similar, while the sample points of Formula 3 are completely independent, indicating that its chemical composition group is significantly different from the former two, forming a new material basis.

[0044] Performance Test 2: Free Radical Scavenging Effect The total antioxidant capacity of formulations one, two, and three was tested as follows: Accurately pipette 0.1 mL of sample solution and dilute to 1 mL with 80% ethanol. Take 10 µL of the test solution, add 200 µL of ABTS working solution, mix gently, incubate at room temperature for 5 min, and then measure the absorbance at 734 nm. For the blank control, use distilled water instead of the test solution; the remaining procedures are the same. Record the measured absorbance (A). 测定) and blank absorbance (A) 空白 ), calculate ΔA 测定 =A 空白 -A 测定 .

[0045] Take 10 μmol / mL Trolox standard solution and dilute it with distilled water to prepare a series of concentrations of 0.70, 0.60, 0.40, 0.20, 0.10, and 0.05 μmol / mL. Perform the standard solution and the test solution simultaneously, and measure the absorbance at 734 nm (A). 标准 ), calculate ΔA 标准 = A 空白 -A_ 标准 Plotting Trolox concentration (μmol / mL) on the x-axis, ΔA 标准 Use the vertical axis to plot the standard curve.

[0046] The total antioxidant capacity of a sample is expressed as the Trolox concentration required to achieve the same ΔA value, calculated according to Equation 1: Formula 1; In Equation 1: —Sample concentration obtained from the standard curve, in μmol / mL; —Total reaction volume, 0.21 mL; —Sample volume in the reaction, 0.01 mL; —Fixed volume, 1 mL; —Sampling volume, 0.1 mL; —Dilution factor.

[0047] The total antioxidant capacity of different groups of samples was determined using the ABTS method. The comparison of the total antioxidant capacity of the three tea formulas by ABTS is shown in the figure below. Figure 2 As shown, by Figure 2 It can be seen that the concentration of ABTS free radicals is 60.19 μmol / mL for formulation 1, 31.61 μmol / mL for formulation 2, and 112.55 μmol / mL for formulation 3. Among them, formulation 3 has the strongest ABTS free radical scavenging ability (ΔA = 112.55 μmol / mL), which is significantly higher than that of formulation 1 (ΔA = 60.19 μmol / mL) and formulation 2 (ΔA = 31.61 μmol / mL). Formulation 1 is the most effective, while formulation 2 has the lowest antioxidant capacity. This indicates that formulation 3 can significantly enhance the ABTS free radical scavenging ability of the system.

[0048] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An antioxidant composition, characterized in that, The components include the following parts by weight: Peach blossoms: 0.5-1 part; 1-2 portions of roses; Kunlun snow chrysanthemum 0.5~1 part; Osmanthus flowers 0.5-1 part; Jasmine flowers, 0.5-1.5 parts; 0.5 to 1.5 parts of mimosa flowers.

2. The antioxidant composition according to claim 1, characterized in that, The components include the following parts by weight: 0.5 portions of peach blossoms; One serving of roses; Kunlun Snow Chrysanthemum 0.5 parts; Osmanthus flowers, 0.6 parts; One serving of jasmine flowers; One portion of mimosa flowers.

3. The antioxidant composition according to claim 1 or 2, characterized in that, The roses mentioned are double-petaled red roses.

4. The antioxidant composition according to claim 1 or 2, characterized in that, The moisture content of the peach blossoms, roses, Kunlun snow chrysanthemums, osmanthus, jasmine, or mimosa flowers is ≤9%.

5. The use of the antioxidant composition according to any one of claims 1 to 4 in the preparation of antioxidant teas, antioxidant drugs or antioxidant health products.

6. An antioxidant tea beverage, characterized in that, It is obtained by brewing the antioxidant composition according to any one of claims 1 to 5.

7. The antioxidant tea beverage according to claim 6, characterized in that, The water used for brewing is boiling water.

8. The antioxidant tea beverage according to claim 6, characterized in that, The steeping time is 20-40 minutes.

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