Sports nutrition powder with the function of assisting uric acid reduction

Abstract

The application discloses a sports nutrition powder formula which is helpful for reducing uric acid and a preparation method thereof. The sports nutrition powder takes slow-release carbohydrate oligomaltose and high-branching cyclic dextrin as main raw materials, is mixed with dietary fiber inulin, a uric acid-reducing functional raw material, a quadruple probiotic powder and other ingredients in a uniform manner through an IBC mixer with a conical barrel, is filled with inert gas, and is heat-sealed to form the sports nutrition powder. The product contains a plurality of natural active ingredients which are homologous to both medicine and food, can realize the uric acid-reducing effect in multiple ways such as reducing uric acid synthesis, promoting uric acid metabolism and anti-inflammation. Through the observation of the intervention effect on a high uric acid mouse model, it is proved that the sports nutrition powder is helpful for reducing the uric acid level.

Description

Technical Field

[0001] This invention belongs to the field of sports nutrition technology, specifically relating to a sports nutrition powder with the function of assisting in lowering uric acid and its preparation method. Background Technology

[0002] Uric acid is a metabolic product of the human body, mainly produced by the liver from purine compounds ingested from food and broken down in the body, and excreted through the kidneys and digestive tract. Under normal circumstances, the production and excretion of uric acid in the body are in balance. When the body's purine metabolism is disordered, resulting in excessive uric acid production and / or a weakened ability to excrete uric acid, the concentration of uric acid in the blood becomes too high, which leads to hyperuricemia (HUA).

[0003] Currently, various raw materials mainly achieve the effects of lowering uric acid and relieving gout through the following three aspects: (1) regulating the expression of uric acid transporters and promoting uric acid excretion; (2) inhibiting xanthine oxidase activity, thereby reducing uric acid production; (3) inhibiting the chemotaxis of inflammatory cells and the release of inflammatory factors, thereby reducing pain caused by inflammation. For example, the ethanol extract and water extract of Poria cocos exert anti-HUA effects mainly by upregulating the expression of renal urate transporters ABCG2, OAT1 and OCT2 and downregulating the expression of renal urate transporter 1 (URAT1) to inhibit uric acid reabsorption, promote renal uric acid excretion, thereby reducing serum uric acid levels and relieving the damage to the body caused by hyperuricemia. In addition, Poria cocos reduces the level of inflammation in the body and improves renal function abnormalities caused by hyperuricemia. Pueraria lobata isoflavones can promote uric acid excretion while also inhibiting xanthine oxidase activity, upregulating GLUT9 expression, and reducing uric acid production. Gardenia contains geniposide, which has the effects of promoting bile and pancreatic secretion, improving liver and gastrointestinal function, and enhancing the body's disease resistance.

[0004] The pathogenesis of hyperuricemia is closely related to gut microbiota dysbiosis. Ingesting probiotics can regulate the gut microbiota by increasing the number of lactobacilli and bifidobacteria in the gut, improving intestinal barrier function, reducing the entry of endotoxins and other metabolites into the liver via the bloodstream, and effectively lowering blood uric acid levels. Studies have found that some probiotics have significant uric acid-lowering effects, such as *Lactobacillus rhamnosus*, *Lactobacillus paracasei*, and *Lactobacillus gasseri*, which can reduce serum uric acid levels while improving kidney function and increasing gut microbiota diversity.

[0005] In addition, exercise has a significant impact on uric acid metabolism. Current clinical treatment guidelines for hyperuricemia both domestically and internationally recommend that doctors, while prescribing medication, also advise patients to maintain a regular exercise routine. According to the National Health Commission's Guidelines for Nutrition and Exercise in Hyperuricemia (2024 Edition), patients with hyperuricemia should primarily engage in aerobic exercise, with at least 150 minutes of moderate-intensity aerobic exercise per week, including forms such as jogging, brisk walking, cycling, and swimming.

[0006] According to GB 24154-2015, the National Food Safety Standard for Sports Nutrition Foods, published in 2015, sports nutrition foods are specially processed foods designed to meet the physiological metabolic state, athletic ability, and specific nutritional needs of people who participate in physical exercise at least three times a week, for at least 30 minutes each time, and at a moderate to high intensity. Currently, commercially available uric acid-lowering products are mainly ordinary foods, and research on sports nutrition supplements specifically for patients with hyperuricemia during exercise is limited. Therefore, developing a sports nutrition supplement with auxiliary uric acid-lowering functions is of great significance for the daily exercise of patients with hyperuricemia.

[0007] This product uses slow-release carbohydrates as its main ingredient, combined with various uric acid-lowering components and probiotics. It is mixed using a square cone IBC mixer and then packaged in nitrogen to produce a sports nutrition food. Animal experiments have confirmed that this sports nutrition powder has an auxiliary effect in lowering uric acid levels. Summary of the Invention

[0008] This invention provides a sports nutrition powder special dietary supplement with auxiliary uric acid-lowering function. The raw materials of this nutritional powder include: maltodextrin oligosaccharide, highly branched cyclodextrin, inulin, sour cherry powder, compound medicinal and food homologous powder (Poria cocos powder, kudzu root powder, gardenia powder), apple powder (60% apple polyphenols), compound nutritional fortifiers and supplements (thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, niacin, folic acid, biotin, caffeine), four probiotics (Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus rhamnosus), silicon dioxide, and taste modifiers.

[0009] Preferably, the sports nutrition powder, by weight, comprises: 200-400g of oligomaltose, 100-300g of highly branched cyclodextrin, 100-200g of inulin, 200-400g of compound medicinal and edible powder, 100-200g of sour cherry powder, 10-30g of compound nutritional fortifiers and supplements, 10-18g of quadruple probiotic powder, 1-15g of silicon dioxide, 1-20g of taste modifier, and 0-10g of apple powder (60% apple polyphenols).

[0010] Preferably, the compound medicinal and edible powder is obtained by blending three medicinal and edible powders in a certain mass ratio, with the following addition per kilogram: 30-100g of Poria cocos powder, 20-80g of Pueraria lobata powder, and 10-55g of Gardenia jasminoides powder. The ethanol and water extracts of Poria cocos exert their anti-HUA effect mainly by upregulating the expression of renal urate transporters ABCG2, OAT1, and OCT2 and downregulating the expression of renal urate transporter 1 (URAT1), thereby inhibiting uric acid reabsorption, promoting renal uric acid excretion, and thus reducing serum uric acid levels and alleviating the damage caused by hyperuricemia. In addition, Poria cocos reduces the level of inflammation in the body and improves renal function abnormalities caused by hyperuricemia. Pueraria lobata isoflavones can promote uric acid excretion while also inhibiting xanthine oxidase activity, upregulating GLUT9 expression, and reducing uric acid production. Gardenia jasminoides contains geniposide, which has the effects of promoting bile and pancreatic secretion, improving liver and gastrointestinal function, and enhancing the body's disease resistance.

[0011] Preferably, the compound nutritional fortifier supplement is obtained by compounding seven vitamins and fortifiers in a certain mass ratio, with the following amounts added per kilogram: 0.02-0.03g thiamine hydrochloride, 0.02-0.03g riboflavin, 0.02-0.03g pyridoxine hydrochloride, 0.00004-0.00005g cyanocobalamin, 0.1-0.15g niacin, 0.001-0.002g folic acid, 0.0001-0.0003g biotin, and 0.01-1g caffeine. Caffeine is a methylxanthine that can inhibit xanthine oxidase activity, thereby reducing uric acid production.

[0012] Preferably, the quadruple probiotic powder is formulated with four types of probiotics in a specific ratio, with the following dosage per kilogram: *Lactobacillus rhamnosus* 0.00001-0.00005g, *Lactobacillus reuteri* 0.00001-0.00004g, *Lactobacillus gasseri* 0.00001-0.00004g, and *Lactobacillus paracasei* 0.00001-0.00005g, for a total live bacteria count of 10. 9 -10 11 CFU / g. Ingesting probiotics can regulate the gut microbiota by increasing the number of lactobacilli and bifidobacteria in the intestines, improving intestinal barrier function, reducing the entry of endotoxins and other metabolites into the liver via the bloodstream, and effectively lowering blood uric acid levels.

[0013] Preferably, the taste modifier includes one or a combination of sucralose, steviol glycosides, citric acid, DL-malic acid, food flavorings, and fruit powder.

[0014] This invention also provides a method for preparing the above-mentioned sports nutrition powder, the steps of which include: Weigh out the following ingredients according to the specified amounts: maltodextrin, high branched-chain cyclodextrin, inulin, sour cherry powder, poria cocos powder, kudzu root powder, gardenia powder, apple powder (60% apple polyphenols), compound nutritional fortifier, four-in-one probiotic powder, silicon dioxide, sucralose, citric acid, DL-malic acid, and food flavoring. Then, pass the mixture through a 30-mesh sieve.

[0015] Mixing: Using an equal-incremental mixing method, first mix the compound nutritional fortifier, nutritional supplement, four-in-one probiotic powder, silicon dioxide, sucralose, citric acid, DL-malic acid, food flavoring, apple powder (60% apple polyphenols) with an equal amount of inulin for 10 minutes to obtain mixed powder I. Mixed powder I is then mixed with the remaining materials for 30 minutes until uniform to obtain the total mixed powder.

[0016] Mixing Equipment and Parameters: The weighed material is fed through a vibrating screen into the IBC (Integrated Batteries Cone) mixer using a vacuum feeder. The working principle of the IBC mixer is based on the fact that the rotation axis of the IBC forms a certain angle with its axis of symmetry. During mixing, the powder undergoes complex three-dimensional motion within the rotating drum. Within one revolution, it completes contraction, diffusion, and convection, thereby achieving the required mixing process. The inner surface of the IBC mixer is smooth, without blades, and the corners are rounded. Typically, the IBC mixer has a loading coefficient of 30%-80% and a working speed of 5-15 rpm. Preferably, the loading coefficient is 70% and the working speed is 10 rpm, resulting in a high degree of material uniformity.

[0017] Inert gas filling: The mixed powder after IBC treatment in a square cone barrel is packaged by a fully automatic bagging and filling machine under nitrogen mediated by a gas flow rate of 600-1000L / h and a gas pressure of 0.6-1MPa, and then heat-sealed to obtain sports nutrition powder with the function of assisting in lowering uric acid, while controlling its residual oxygen content to <3%. Detailed Implementation

[0018] The present invention will now be clearly and completely described with reference to specific embodiments, but the described embodiments are only a part of the present invention. Unless otherwise specified, the raw materials, methods, and equipment used in the present invention are commonly used in this technical field. Unless otherwise specified, all raw materials used in the present invention are commercially available. Example 1

[0019] A method for preparing a sports nutrition powder with the function of assisting in lowering uric acid includes: (1) weighing 46.8 kg of oligomaltose, 30.6 kg of high branched cyclodextrin, 26.28 kg of inulin, 27 kg of sour cherry powder, 39.384 kg of compound food and medicine homology powder, 0.936 kg of apple powder (60% apple polyphenols), 1.8 kg of compound nutritional fortifier and nutritional supplement, 1.8 kg of quadruple probiotic powder, 1.8 kg of silicon dioxide, 0.1 kg of sucralose, 0.15 kg of citric acid, 0.1 kg of DL-malic acid, and 0.15 kg of food flavoring, totaling 180 kg; the above materials are passed through a 30-mesh sieve for later use. (2) Apple powder (60% apple polyphenols), compound nutritional fortifier, four-in-one probiotic powder, silicon dioxide, sucralose, citric acid, DL-malic acid, food flavoring and an equal amount of inulin are premixed. (3) All materials are put into a square cone barrel IBC mixer with a loading coefficient of 70% and a working speed of 10 rpm. The mixed powder is made by quantitative filling and heat sealing under nitrogen mediated by 0.8 MPa and 800 L / h. The filling amount is controlled at 25 ± 0.5 g / bag, the filling accuracy is above 99.9%, and the residual oxygen content of the product is <2%, thus obtaining a sports nutrition food that can help lower uric acid. Example 2

[0020] Construction and efficacy testing of a mouse model of hyperuricemia Uric acid oxidase can convert uric acid into water-soluble allantoin, which is excreted in urine. Under natural conditions, mice do not suffer from hyperuricemia because they have uric acid oxidase in their bodies. Potassium oxonate (PO) is a uric acid oxidase inhibitor, which inhibits the decomposition of uric acid and thus accumulates in mice. Therefore, potassium oxonate is often used to treat mice to establish a hyperuricemia mouse model.

[0021] The effects of this sports nutrition powder on uric acid, creatinine, blood urea nitrogen levels and xanthine oxidase activity in hyperuricemic mice were evaluated. Several SPF-grade mice were used and fed free access to food and water under conditions of 22-24℃, 50%-70% relative humidity, and a 12-hour light cycle. After one week of acclimatization, the mice were randomly divided into three groups (control group, positive control group, and experimental group), with 10 mice in each group. The control group was injected intraperitoneally with a 0.5% sodium carboxymethyl cellulose solution, while the other two groups were injected intraperitoneally with 400 mg / (kg·d) PO dissolved in a 0.5% sodium carboxymethyl cellulose solution. One hour later, the control and positive control groups were administered physiological saline by gavage, while the experimental group was administered the sports nutrition powder prepared in Example 1 by gavage. This treatment was continued for one month, during which all mice underwent supplementary exercise. Twelve hours prior to sampling, mice were fasted but allowed free access to water. Two hours after the last administration, five mice were randomly selected from each model group. Blood was collected from the eyeballs after ether anesthesia. The blood was centrifuged at 3000 rpm for 10 minutes at 4°C. The supernatant serum was collected to detect the levels of uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN), and xanthine oxidase (XOD). Serum creatinine and BUN levels reflect kidney function, and xanthine oxidase is involved in purine metabolism to produce uric acid. All oral gavage and intraperitoneal injections were performed at a dose of 0.4 mL / 20 g.

[0022] Hyperuricemia Model Construction and Efficacy Testing Protocol Group processing method control group Intraperitoneal injection of 0.5% sodium carboxymethyl cellulose solution + normal saline via gavage Positive control group Intraperitoneal injection of 400 mg / (kg·d) PO solution + normal saline via gavage experimental group Intraperitoneal injection of 400 mg / (kg·d) PO solution + sports nutrition powder via gavage The effects of this sports nutrition powder intervention on serum uric acid, creatinine, blood urea nitrogen levels and xanthine oxidase activity in hyperuricemic model mice (x±s, n=5) Group UA (µmol / L) Cr (µmol / L) BUN (mmol / L) XOD (U / L) control group 97.96 ± 0.80 c ]] <![CDATA[8.92±0.48 c ]]> <![CDATA[4.42±0.37 c ]]> <![CDATA[18.87±0.56 c ]]> Positive control group <![CDATA[216.53±2.18 a ]]> <![CDATA[19.55±0.67 a ]]> <![CDATA[9.37±0.51 a ]]> <![CDATA[29.89±0.64 a ]]> experimental group <![CDATA[167.39±1.43 b ]]> <![CDATA[12.61±0.90 b ]]> <![CDATA[6.22±0.41 b ]]> <![CDATA[21.92±0.90 b ]]> Note: Different letters between groups represent significant differences. p<0.05 ).

[0023] As can be seen from the table above, after the modeling process, the serum uric acid level of mice injected intraperitoneally with 400 mg / (kg·d) PO solution was 216.53 µmol / L, which was significantly higher than that of the control group (97.96 µmol / L). p<0.05The uric acid level in mice treated with the sports nutrition powder was approximately 2.2 times that of the control group. After one month of gavage treatment, the serum uric acid level in mice treated with the sports nutrition powder was 167.39 µmol / L, which was lower than that in the untreated group, but still higher than the normal uric acid value, indicating that the sports nutrition powder prepared in this invention has a certain degree of uric acid-lowering effect. Compared with the control group, the serum creatinine and urea nitrogen levels in the positive control group were significantly increased, reaching 19.55 µmol / L and 9.37 mmol / L, respectively, an increase of 107% and 111%, indicating that high uric acid accumulation caused significant kidney damage in mice. After one month of administration of the sports nutrition powder, the serum creatinine and urea nitrogen levels in the experimental group decreased by 35.5% and 33.6%, respectively, and the kidney damage was improved. In addition, the serum xanthine oxidase activity in mice with high uric acid was also high, reaching 29.89 U / L, which can catalyze the conversion of more xanthine to uric acid. However, the serum xanthine oxidase activity in mice treated with the sports nutrition powder decreased by 26.7%, thereby inhibiting the metabolism of purine to produce uric acid.

[0024] The results showed that the sports nutrition powder of the present invention can reduce the serum uric acid, creatinine and urea nitrogen levels in hyperuricemic mice to a certain extent, alleviate kidney damage, and inhibit serum xanthine oxidase activity, thereby hindering the metabolism of purines into uric acid and reducing uric acid production.

[0025] This specific embodiment is merely an explanation of this application and is not intended to limit it. Various changes and modifications can be made to this invention without departing from its spirit and scope. Any equivalent structural changes to the content of this specification, directly or indirectly applied to other technical fields under the concept of this invention, are included within the patent protection scope of this invention.

Claims

1. A sports nutrition powder for endurance that helps lower uric acid, characterized in that: It uses slow-release carbohydrates as an excipient, combined with a variety of uric acid-lowering functional ingredients and probiotic powder, to achieve the effect of lowering uric acid through multiple pathways such as reducing uric acid production, promoting uric acid metabolism, and anti-inflammation.

2. The endurance sports nutrition powder with auxiliary uric acid-lowering effect according to claim 1, characterized in that, Added per kilogram: 200-400g of oligomaltose, 100-300g of highly branched cyclodextrin, 100-200g of inulin, 200-400g of compound medicinal and edible powder, 100-200g of sour cherry powder, 10-30g of compound nutritional fortifier / supplement, 10-30g of quadruple probiotic powder, 1-15g of silicon dioxide, 1-20g of taste modifier, and 0-10g of apple powder (60% apple polyphenols).

3. The endurance sports nutrition powder with auxiliary uric acid-lowering effect according to claim 2, characterized in that: (1) The compound medicinal and edible powder is obtained by compounding three kinds of medicinal and edible powders in a certain mass ratio. The amount added per kilogram is: 30-100g of Poria cocos powder, 20-80g of Pueraria lobata powder, and 10-55g of Gardenia jasminoides powder. (2) The compound nutritional fortifier is obtained by compounding seven kinds of vitamins and fortifiers in a certain mass ratio. The amount added per kilogram includes: 0.02-0.03g of thiamine hydrochloride, 0.02-0.03g of riboflavin, 0.02-0.03g of pyridoxine hydrochloride, 0.00004-0.00005g of cyanocobalamin, 0.1-0.15g of niacin, 0.001-0.002g of folic acid, 0.0001-0.0003g of biotin, and 0.01-1g of caffeine. (3) The aforementioned quadruple probiotic powder is formulated with four types of probiotics in a specific ratio, with the following amounts added per kilogram: Lactobacillus rhamnosus 0.00001-0.00005 g, Lactobacillus reuteri 0.00001-0.00004 g, Lactobacillus gasseri 0.00001-0.00004 g, and Lactobacillus paracasei 0.00001-0.00005 g, for a total live bacteria count of 10. 9 -10 11 The mouthfeel modifiers described in cfu / g. (4) include one or more of the following: sucralose, steviol glycosides, citric acid, DL-malic acid, food flavorings, and fruit powder.

4. The endurance sports nutrition powder with auxiliary uric acid-lowering effect according to claim 2, characterized in that, Includes the following steps: (1) Weigh out the following ingredients according to the specified proportions: maltodextrin, high-branched cyclodextrin, inulin, sour cherry powder, poria cocos powder, kudzu root powder, gardenia powder, apple powder (60% apple polyphenols), compound nutritional fortifier, nutritional supplement, four-in-one probiotic powder, silicon dioxide, sucralose, citric acid, DL-malic acid, and food flavoring. Sieve through a 30-mesh sieve for later use. (2) Pass the weighed materials through a vibrating screen and use a vacuum feeder to feed them into the square cone barrel IBC mixer. The working principle of the IBC mixer is that the rotation axis of the IBC forms a certain angle with the axis of symmetry of the IBC. During mixing, the powder undergoes complex three-dimensional motion in the rotating drum. Within one rotation, it completes the shrinkage, diffusion, and convection motions, thereby achieving the mixing process requirements. The inner surface of the IBC mixer is smooth, without blades, and the corners are rounded. Typically, the loading coefficient of the IBC mixer is 30%-80%, and the working speed is 5-15 rpm. Preferably, the loading coefficient is 70% and the working speed is 10 rpm, resulting in a high degree of material mixing uniformity. (3) The mixed powder after IBC treatment in the square cone barrel is packaged by a fully automatic bag filling machine under the nitrogen-mediated gas flow rate of 600-1000L / h and gas pressure of 0.6-1MPa. After heat sealing, sports nutrition powder with auxiliary uric acid lowering effect is obtained. The quantitative filling is 25g±0.5g / bag, the yield is >99.5%, and the residual oxygen content is <3%.

5. The sports nutrition powder for assisting in lowering uric acid as described in claim 1, characterized in that: Application of the sports nutrition powder with uric acid-lowering function in animal models.