Animal-plant double protein composite powder and preparation method thereof

Abstract

This invention discloses a plant-animal dual-protein compound powder and its preparation method, belonging to the field of health food technology. The compound powder, by weight, consists of 780-820 parts soy protein isolate, 60-80 parts whey protein powder, 25-35 parts fructooligosaccharides, 8-12 parts galactooligosaccharides, and 8-12 parts silicon dioxide. The preparation method employs a five-stage, stepwise, equal-volume incremental mixing process. First, a trace amount of galactooligosaccharides is premixed with silicon dioxide, then sequentially mixed with fructooligosaccharides, whey protein powder, and soy protein isolate. Finally, it is dried at a low temperature of 70-75℃ to control the moisture content to ≤5.0%. This invention, through the scientific compounding of plant and animal proteins and the synergistic effect of two prebiotics, comprehensively enhances the body's immunity from three dimensions: nutritional supplementation, intestinal immune regulation, and antioxidant effects. The unique five-stage mixing process solves the industry problem of uneven mixing of multi-component raw materials with large proportions of differences.

Description

Technical Field

[0001] This invention belongs to the field of health food technology, specifically relating to a compound powder of animal and plant proteins and its preparation method. Background Technology

[0002] Immunity is the core defense mechanism of the human body against the invasion of external pathogens and the maintenance of internal homeostasis. With the fast pace of modern life, increased work pressure, and unbalanced diets, weakened immunity is becoming increasingly common, manifesting as symptoms such as susceptibility to colds, fatigue, and slow recovery. Protein is the building block of the immune system; insufficient intake or poor quality protein directly leads to inhibited immune cell proliferation and decreased antibody synthesis. Therefore, supplementing with high-quality protein is an effective way to enhance immunity.

[0003] Currently, most protein powders on the market use only soy protein or whey protein as raw materials, resulting in an unbalanced amino acid composition and a lack of regulatory effects on intestinal immunity. The gut is the largest immune organ in the human body, with over 70% of immune cells concentrated there. Single-protein formulas cannot achieve a synergistic enhancement of both systemic and intestinal immunity.

[0004] Therefore, developing a scientifically formulated, uniformly mixed, stable, additive-free, and significantly immune-enhancing compound powder of animal and plant proteins, along with its preparation method, has significant market value and social significance. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a plant and animal dual protein composite powder and its preparation method, solving the technical problems of poor formulation synergy, uneven mixing of raw materials with large differences in proportion of multiple components, high product moisture content, and short shelf life in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A compound powder of animal and plant proteins, by weight, is composed of 780-820 parts of soy protein isolate, 60-80 parts of whey protein powder, 25-35 parts of fructooligosaccharides, 8-12 parts of galactooligosaccharides, and 8-12 parts of silicon dioxide.

[0007] Furthermore, the soy protein isolate has a protein content of ≥90%, conforming to GB20371-2016 standard; the whey protein powder has a protein content of ≥80%, conforming to GB11674-2010 standard; the fructooligosaccharide content is ≥90%, conforming to GB / T23528.2-2021 standard; the galactooligosaccharide content is ≥57% (dry basis); and the silicon dioxide conforms to GB25576-2010 standard.

[0008] This invention also provides a method for preparing the above-mentioned animal and plant dual-protein composite powder, comprising the following steps: S1. Raw material pretreatment: Soy protein isolate, whey protein powder, fructooligosaccharides, galactooligosaccharides, and silicon dioxide are sieved through a mesh screen to remove impurities and clumps. After passing the inspection, they are ready for use. S2, Primary Premix: Weigh out the oligogalactose and an equal amount of silicon dioxide according to the formula, place them in a three-dimensional motion mixer and grind and mix until the color is uniform to obtain mixture 1; S3, Secondary mixing: Add the remaining silica to mixture 1 and mix to obtain mixture 2; S4. Tertiary mixing: Add fructooligosaccharides to mixture 2 and mix to obtain mixture 3; S5, Fourth-stage mixing: Add whey protein powder to mixture 3 and mix to obtain mixture 4; S6. Five-stage equal-quantity incremental mixing: Mixture 4 and soy protein isolate are gradually mixed using the equal-quantity incremental method. Each time, an equal amount of soy protein isolate is added to the current mixture. The mixture is continued until all the soy protein isolate is added. Finally, the mixture is mixed to obtain the total powder. S7. Low-temperature drying: Place the total mixed powder in a hot air circulating oven and dry it at 70-75℃ until the moisture content is ≤5.0%, then cool it to room temperature; S8. Repackaging and outer packaging: The dried materials are repackaged in a Class 300,000 clean area.

[0009] As an improvement, in the S1 raw material pretreatment, all raw materials are passed through a 100-mesh sieve.

[0010] As an improvement, in the S2 primary premix, galactooligosaccharides and an equal amount of silica are weighed according to the formula, placed in a three-dimensional motion mixer, and ground and mixed at a speed of 15-20 r / min in an environment of 18-26℃ and relative humidity ≤60% until the color is uniform, to obtain mixture 1.

[0011] As an improvement, the remaining silica was added to mixture 1 during the S3 secondary mixing process, and mixing was continued for 10 minutes to obtain mixture 2.

[0012] As an improvement, in the S4 three-stage mixing process, oligofructose was added to mixture 2 and mixed for 10 minutes to obtain mixture 3.

[0013] As an improvement, whey protein powder was added to mixture 3 during the S5 four-stage mixing process, and the mixture was mixed for 15 minutes to obtain mixture 4.

[0014] As an improvement, in the S6 five-stage equal incremental mixing, the equal incremental method is used to gradually mix mixture 4 with soy protein isolate. Each time, an equal amount of soy protein isolate is added to the current mixture, and the mixture is mixed for 10 minutes until all the soy protein isolate is added. Finally, the mixture is mixed for another 15 minutes to obtain the total mixed powder.

[0015] As an improvement, in the S7 low-temperature drying process, the total mixed powder is placed in a hot air circulating oven and dried at 70-75℃. The material is turned over every 30 minutes until the moisture content is ≤5.0%, and then it is taken out and cooled to room temperature.

[0016] As an improvement, the dried material is conveyed to a fully automatic powder filling machine in a Class 300,000 clean area during the S8 dispensing and outer packaging process. It is then dispensed in 450g / cans, sealed, and the filling volume difference is controlled within ±3.0%. Then, the outer packaging is carried out, and a full inspection is conducted according to the enterprise standard. After passing the inspection, the material is stored in the warehouse. Beneficial effects

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) This invention uses a golden ratio of 78:7 of soy protein isolate and whey protein, with an amino acid composition close to the human body’s needs and a digestibility rate of over 95%, providing high-quality raw materials for immune cell and antibody synthesis; the addition of fructooligosaccharides and galactooligosaccharides as prebiotics can selectively proliferate beneficial bacteria such as bifidobacteria and lactic acid bacteria in the intestine, regulate the balance of intestinal flora, and enhance the intestinal mucosal immune barrier; the three work synergistically to achieve a dual enhancement of systemic immunity and intestinal immunity, with a significantly better effect than single protein products.

[0018] (2) To address the issue of significant differences in proportions between trace components such as galactooligosaccharides (1%) and silicon dioxide (1%) and the main raw material, soy protein isolate (80%), this invention designs a five-stage stepwise mixing process. First, the trace amounts of galactooligosaccharides and silicon dioxide are premixed, and then the mixing system is gradually expanded to ensure uniform dispersion of the trace components in the main raw material. Experiments have shown that the protein content RSD of the product prepared by this process is only 1.12%, far lower than the 7.85% of the conventional direct mixing process. This completely solves the technical problem of uneven mixing of multi-component raw materials with large proportion differences, ensuring the consistency of efficacy for each product.

[0019] (3) This invention determines the optimal drying temperature of 70-75℃, which can effectively remove moisture and control it below 5.0%, while avoiding protein denaturation and prebiotic degradation. At the same time, the relative humidity of the production environment is strictly controlled to ≤60%, which effectively reduces the hygroscopicity of the product. Accelerated test results show that the product of this invention, after being placed at 40℃ and 75% relative humidity for 6 months, shows no significant changes in various indicators, and the shelf life can reach 24 months.

[0020] (4) All raw materials of this invention are ordinary food or food additives, and do not contain artificial additives such as flavorings, sweeteners, and preservatives. According to the safety evaluation experiment of Hunan Provincial Center for Disease Control and Prevention, they are safe and non-toxic within the recommended dosage range and are suitable for long-term use.

[0021] The preparation process of this invention does not require complex equipment, is easy to operate, and the yield of three batches of pilot products is ≥97%. The process has good reproducibility and is suitable for large-scale industrial production. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to specific embodiments. Example 1

[0023] This embodiment provides a plant and animal dual protein compound powder with the following formula: 800g of soy protein isolate (protein content 90.5%), 70g of whey protein powder (protein content 82.3%), 30g of fructooligosaccharides (content 92.1%), 10g of galactooligosaccharides (content 58.5%), and 10g of silicon dioxide.

[0024] The preparation method is as follows: S1. Raw material pretreatment: Pass the above raw materials through a 100-mesh sieve and use them after passing the inspection. S2, Primary premix: Weigh 10g of galactooligosaccharide and 10g of silicon dioxide, place them in a three-dimensional motion mixer, and grind and mix them at a speed of 18r / min in an environment of 22℃ and 55% relative humidity until the color is uniform, to obtain mixture 1 (20g). S3, Secondary mixing: Add the remaining 0g of silica to mixture 1 and mix for 10 minutes to obtain mixture 2 (20g). S4. Three-stage mixing: Add 30g of oligofructose to mixture 2 and mix for 10 minutes to obtain mixture 3 (50g). S5, Fourth stage mixing: Add 70g of whey protein powder to mixture 3 and mix for 15 minutes to obtain mixture 4 (120g). S6. Five-stage equal-incremental mixing: (1) Add 120g of soy protein isolate and mix for 10 minutes to obtain mixed powder A1 (240g); (2) Add 240g of soy protein isolate and mix for 10 minutes to obtain mixed powder A2 (480g); (3) Add 480g of soy protein isolate and mix for 10 minutes to obtain mixed powder A3 (960g); (4) Add the remaining 0g of soy protein isolate and mix for 15 minutes to obtain total mixed powder (960g). S7. Low-temperature drying: Place the total mixed powder in a hot air circulating oven and dry at 72°C, turning it over every 30 minutes until the moisture content is ≤5.0%. Remove and cool to room temperature. S8. Dispensing and outer packaging: In a Class 300,000 clean area, use a fully automatic powder filling machine to dispense powder at 450g / can, seal it, and control the filling volume difference within ±3.0%; then carry out outer packaging, and put it into storage after passing inspection. Example 2

[0025] This embodiment provides a compound powder of animal and plant proteins, with the following formula: 780g soy protein isolate, 80g whey protein powder, 35g fructooligosaccharides, 12g galactooligosaccharides, and 12g silicon dioxide.

[0026] The preparation method is the same as in Example 1. Example 3

[0027] This embodiment provides a plant-animal dual-protein composite powder with the following formula: 820g soy protein isolate, 60g whey protein powder, 25g fructooligosaccharides, 8g galactooligosaccharides, and 8g silicon dioxide.

[0028] The preparation method is the same as in Example 1. Comparative Example 1

[0029] Prepared using a conventional direct mixing process, with the same formula as in Example 1: 930g of soy protein isolate, 70g of whey protein powder, 30g of fructooligosaccharides, 10g of galactooligosaccharides, and 10g of silica were added to a mixer at once and mixed for 45 minutes. The remaining steps were the same as in Example 1. Example 4: Comparison Experiment on Mixing Uniformity

[0030] Six samples were randomly selected from the total mixed powder of Example 1 and Comparative Example 1, respectively. The protein content was determined according to GB5009.5-2016 "National Food Safety Standard - Determination of Protein in Food", and the RSD value was calculated. The results are shown in the table below: 1 81.23 76.54 2 80.95 85.32 3 81.56 78.91 4 80.78 83.67 5 81.34 77.25 6 80.89 82.48 average value 81.13 80.70 RSD (%) 1.12 7.85 Experimental results show that the five-stage stepwise equal-volume incremental mixing process of the present invention significantly improves the mixing uniformity of the product, with minimal fluctuation in protein content, thus ensuring the consistency of product quality. Example 5: Three batches of pilot-scale testing

[0031] Following the formula and process of Example 1, three batches of pilot production were conducted, with a feed amount of 45 kg (100 cans) for each batch. The production results are shown in the table below: 20260101 45.0 45.0 43.65 97.0 81.08 1.08 4.2 20260102 45.0 45.0 43.20 96.0 81.15 1.15 4.3 20260103 45.0 45.0 43.65 97.0 81.11 1.09 4.1 All three batches of pilot products met the enterprise's standard requirements, with a yield rate of ≥96%, indicating that the process of this invention is stable and reliable, and suitable for large-scale industrial production. Example 6: Evaluation Test of Enhanced Immunological Function

[0032] In accordance with the requirements of the "Technical Specifications for Inspection and Evaluation of Health Foods" (2003 edition), the Hunan Provincial Center for Disease Control and Prevention was commissioned to conduct a mouse immune enhancement function test on the product prepared in Example 1.

[0033] 1. Experimental animals: 60 male SPF-grade Kunming mice, weighing 18-22g.

[0034] 2. Experimental grouping: Mice were randomly divided into 4 groups of 15 each: blank control group, low-dose group (0.75g / kg), medium-dose group (1.5g / kg), and high-dose group (3.0g / kg).

[0035] 3. Experimental Methods: Mice in each treatment group were administered the corresponding test substance by gavage daily, while the blank control group was administered an equal volume of physiological saline by gavage for 30 consecutive days. After the last administration, delayed-type hypersensitivity (DTH), antibody-producing cell count, serum hemolysin level, peritoneal macrophage phagocytic capacity for chicken erythrocytes, and NK cell activity were measured in mice.

[0036] 4. Experimental Results: Blank control group 0.22±0.06 24.1±3.5 105.3±13.2 33.5±4.3 19.2±2.5 low-dose group 0.30±0.07 32.5±4.8 128.7±16.5 43.2±5.4 24.8±3.2 medium dose group 0.37±0.08 40.2±5.3 146.5±19.3 50.6±6.5 29.7±3.8 High-dose group 0.44±0.09 47.5±6.1 162.4±22.5 58.3±7.2 34.5±4.3 Note: Compared with the blank control group, *P<0.05, **P<0.01.

[0037] The experimental results show that the product of this invention can significantly enhance cellular immunity, humoral immunity, mononuclear-macrophage function and NK cell activity in mice, meeting the criteria for enhancing immune function in the "Technical Specifications for Inspection and Evaluation of Health Foods". Example 7 Accelerated Stability Test

[0038] The product prepared in Example 1 was subjected to an accelerated stability test for 6 months at a temperature of 40℃±2℃ and a relative humidity of 75%±5%. Samples were taken and tested at 0, 1, 2, 3, and 6 months, and the results are shown in the table below: Senses Off-white powder, without lumps Off-white powder, without lumps Off-white powder, without lumps Off-white powder, without lumps Off-white powder, without lumps Protein content (g / 100g) 81.13 81.02 80.89 80.75 80.52 Moisture (%) 4.2 4.4 4.6 4.8 5.1 Total bacterial count (CFU / g) 115 128 142 158 175 Coliform bacteria (MPN / g) <0.03 <0.03 <0.03 <0.03 <0.03 Accelerated testing results show that the product of this invention, after being placed under accelerated conditions for 6 months, meets all standard requirements and exhibits stable quality.

[0039] The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A compound powder of animal and plant proteins, characterized in that: By weight, it is composed of the following raw and auxiliary materials: 780-820 parts soy protein isolate, 60-80 parts whey protein powder, 25-35 parts fructooligosaccharides, 8-12 parts galactooligosaccharides, and 8-12 parts silicon dioxide; the soy protein isolate has a protein content ≥90%; the whey protein powder has a protein content ≥80%; the fructooligosaccharide content is ≥90%; and the galactooligosaccharide content is ≥57%.

2. The animal and plant dual-protein composite powder according to claim 1, characterized in that, By weight, it consists of the following raw and auxiliary materials: 800 parts soy protein isolate, 70 parts whey protein powder, 30 parts fructooligosaccharides, 10 parts galactooligosaccharides, and 10 parts silicon dioxide.

3. The animal and plant dual-protein composite powder according to claim 1 or 2, characterized in that, The defining component of the composite powder is protein, with a protein content of ≥80g per 100g of product.

4. The animal and plant dual-protein composite powder according to claim 1 or 2, characterized in that, The composite powder is a uniform powder ranging from off-white to pale yellow, with the unique taste and odor of this product. It does not absorb moisture or clump, and there are no foreign objects visible to normal vision. The moisture content is ≤8.0%, and the ash content is ≤10.0%.

5. A method for preparing the animal and plant dual-protein composite powder as described in any one of claims 1-4, characterized in that, Includes the following steps: S1. Raw material pretreatment: Soy protein isolate, whey protein powder, fructooligosaccharides, galactooligosaccharides, and silicon dioxide are sieved through a mesh screen to remove impurities and clumps. After passing the inspection, they are ready for use. S2, Primary Premix: Weigh out the oligogalactose and an equal amount of silicon dioxide according to the formula, place them in a three-dimensional motion mixer and grind and mix until the color is uniform to obtain mixture 1; S3, Secondary mixing: Add the remaining silica to mixture 1 and mix to obtain mixture 2; S4. Tertiary mixing: Add fructooligosaccharides to mixture 2 and mix to obtain mixture 3; S5, Fourth-stage mixing: Add whey protein powder to mixture 3 and mix to obtain mixture 4; S6. Five-stage equal-quantity incremental mixing: Mixture 4 and soy protein isolate are gradually mixed using the equal-quantity incremental method. Each time, an equal amount of soy protein isolate is added to the current mixture. The mixture is continued until all the soy protein isolate is added. Finally, the mixture is mixed to obtain the total powder. S7. Low-temperature drying: Place the total mixed powder in a hot air circulating oven and dry it at 70-75℃ until the moisture content is ≤5.0%, then cool it to room temperature; S8. Repackaging and outer packaging: The dried materials are repackaged in a Class 300,000 clean area.

6. The preparation method according to claim 5, characterized in that, In steps S2 to S6, the ambient temperature of the mixing process is controlled at 18-26℃ and the relative humidity is controlled below 60%; the rotation speed of the three-dimensional motion mixer is 15-20 r / min.

7. The preparation method according to claim 5, characterized in that, In step S7, the material is turned over every 30 minutes during the drying process to ensure uniform drying.

8. The preparation method according to claim 5, characterized in that, In step S8, the dispensing is carried out using a fully automatic powder filling machine, and the filling volume difference is controlled within ±3.0%.