A tumor vaccine manufacturing apparatus

Abstract

This invention relates to the field of vaccine manufacturing technology, specifically to a tumor vaccine manufacturing device. The device includes a frame on which a feeding module, a separation module, a collection module, and a control system are mounted. These modules are installed in layers from top to bottom along the height of the frame, and are coaxially arranged parallel to the frame's axis. The control system is electrically connected to each module. The separation module includes a shell containing a molecular sieve filling structure. This structure comprises several filling layers with gradient molecular sieve pore sizes, decreasing gradually from the feeding end to the discharging end. Furthermore, the molecular sieve filling density of each layer is negatively correlated with the pore size. This invention addresses the problems of dispersed layout of the feeding, separation, and collection modules and a single separation structure in traditional oncolytic virus tumor vaccine manufacturing equipment.

Description

Technical Field

[0001] This invention relates to the field of vaccine manufacturing technology, specifically to a tumor vaccine manufacturing device. Background Technology

[0002] In the production of oncolytic virus tumor vaccines, the isolation and purification of oncolytic viruses is the core step that determines vaccine quality. The accuracy of isolation and the preservation of viral activity directly affect the subsequent immunization efficacy and clinical application safety of the vaccine. Currently, traditional oncolytic virus tumor vaccine production equipment has a core problem: the layout of the core functional modules is unreasonable. The feeding, separation, and collection modules are mostly distributed, and the separation structure design is simple, which cannot adapt to the isolation requirements of oncolytic viruses.

[0003] Specifically, in traditional equipment, the feeding, separation, and collection modules are dispersed, causing the material to undergo multiple turns during transportation. This not only increases the probability of material retention and deviation but also causes unstable feeding pressure, making it impossible for oncolytic viruses to achieve uniform contact and separation with impurities. At the same time, the separation structure often uses a single-pore molecular sieve filling method, which cannot classify and intercept impurities based on the size differences between oncolytic viruses and impurities of different particle sizes. This either makes it difficult to completely remove small molecule proteins, cell debris, and other impurities, affecting vaccine purity, or it can easily cause excessive interception of oncolytic viruses, resulting in a significant loss of viral activity.

[0004] The aforementioned shortcomings of existing equipment result in low purification efficiency for oncolytic virus tumor vaccines, making it difficult to achieve the required purity and viral activity after purification. This not only increases vaccine production costs but may also affect the subsequent immunization efficacy, failing to meet the demands for large-scale, high-quality production of oncolytic virus tumor vaccines. Therefore, there is an urgent need for tumor vaccine production equipment that can solve these core problems, achieve efficient and precise separation of oncolytic viruses, and preserve viral activity. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a tumor vaccine manufacturing device that solves the core issue in traditional oncolytic virus tumor vaccine manufacturing devices: the dispersed layout of the feeding, separation, and collection modules and the simple separation structure lead to low purity and significant activity loss of the oncolytic virus, failing to meet the high-quality manufacturing requirements of oncolytic virus tumor vaccines.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: a tumor vaccine manufacturing device, comprising a frame, on which a feeding module, a separation module, a collection module and a control system are mounted. The control system is used to coordinate the control of the operating parameters of each module. The feeding module, separation module and collection module are installed in layers from top to bottom along the height direction of the frame. The feeding module, separation module and collection module are arranged coaxially parallel to the axis of the frame. The control system is electrically connected to the feeding module, separation module and collection module respectively.

[0007] The separation module includes a shell, inside which is a molecular sieve filling structure. The molecular sieve filling structure includes several filling layers. The molecular sieve pore size of each filling layer is distributed in a gradient. The molecular sieve pore size gradually decreases from the feed end to the discharge end, and the molecular sieve filling density of each filling layer is negatively correlated with the pore size.

[0008] Furthermore, the top of the housing of the separation module is provided with a feeding interface, and the bottom of the housing is provided with a discharging interface. The feeding interface is coaxially and sealed with the discharging end of the feeding module, and the discharging interface is coaxially and sealed with the feeding end of the collection module. The molecular sieve filling structure also includes a filling frame, which is detachably connected to the inner wall of the housing. The filling frame is provided with several partition plates, which divide the filling frame into several filling cavities. Each filling cavity corresponds to a filling layer. The side wall of the filling cavity is provided with several air vents. The diameter of the air vents is smaller than the molecular sieve pore diameter of the corresponding filling layer. The top of the filling frame is provided with a guide port corresponding to the feeding interface of the housing.

[0009] Furthermore, the inner wall of the shell is provided with a ring-shaped heating layer and several evenly distributed temperature sensors. The temperature sensors are electrically connected to the control system. The heating layer is used to maintain a constant temperature in the separation environment inside the shell. The control system is used to adjust the heating layer according to the real-time temperature monitoring of the temperature sensors. The side wall of the shell is provided with an observation window and a temperature display panel electrically connected to the control system. The observation window is used to observe the usage status of the filling layer in the filling frame in real time. The temperature display panel is used to intuitively display the real-time temperature inside the shell.

[0010] Furthermore, the feeding module includes a feeding hopper, a feeding pump, a feeding pipe, and a flow regulation component. The feeding hopper is fixedly installed on the top of the frame, and its outlet is sealed to the inlet of the feeding pump. The feeding pump is fixedly connected to the side of the top of the frame, and its outlet is coaxially sealed to the inlet of the housing through the feeding pipe. The flow regulation component includes a flow regulation valve and a pressure sensor. Both the flow regulation valve and the pressure sensor are installed on the feeding pipe and electrically connected to the control system. The pressure sensor is used to detect the material pressure in the feeding pipe, and the control system automatically adjusts the opening of the flow regulation valve based on the pressure feedback signal.

[0011] Furthermore, the collection module includes a primary collection tank, a filter assembly, a sampling port, and a discharge valve. The primary collection tank is fixedly connected to the bottom of the frame, and the top inlet and outlet of the primary collection tank are coaxially and sealed. The filter assembly is embedded in the inlet of the primary collection tank and is used to intercept incompletely separated target particles and impurities. The sampling port is located in the middle of the side wall of the primary collection tank and is equipped with a sealed sampling valve, which is used by the experimenter to take samples in real time to detect the concentration and activity of the material. The discharge valve is located at the bottom of the primary collection tank and is used to discharge the purified material.

[0012] Furthermore, a sealing linkage structure is provided between the shell and the filling frame. The sealing linkage structure includes an annular sealing groove, an elastic sealing ring, and a limiting boss. The annular sealing groove is opened on the inner wall of the shell, the elastic sealing ring is embedded in the annular sealing groove, and the limiting boss is fixedly connected to the outer wall of the filling frame.

[0013] Furthermore, the partition plate is provided with several guide holes corresponding to the vent holes. The diameter of the guide holes is the same as the diameter of the vent holes of the corresponding filling chamber, and the guide holes and vent holes are arranged coaxially. The partition plate and the interior of the filling chamber form an annular guide step. The guide step is connected to the guide port at the top of the filling frame. The guide step is used to realize that the material enters from the guide port and is diverted to each filling chamber through the guide step.

[0014] Furthermore, the support feet are fixedly connected to the bottom of the frame. The support feet are used to reduce the vibration generated by the feed pump during equipment operation, and the bottom of the support feet is equipped with anti-slip and wear-resistant pads.

[0015] Furthermore, it also includes an early warning module, which is used to provide real-time warnings for abnormal situations during operation. The early warning module includes indicator lights and a buzzer, both of which are electrically connected to the control system. When the pressure in the feed pipe exceeds the preset pressure threshold in the control system and the temperature inside the shell deviates from the set temperature threshold range, the control system triggers the indicator lights to emit warning lights of the corresponding colors, and the buzzer emits intermittent alarm sounds. At the same time, the control system records abnormal information synchronously.

[0016] Furthermore, the control system is electrically connected to a touch screen and a data storage module. The touch screen is used for parameter setting and real-time monitoring of the operating status, while the data storage module is used to store equipment operating parameters and separation process data.

[0017] The above approach has the following beneficial effects:

[0018] 1. The feeding module, separation module and collection module of this solution are arranged in a top-down layered coaxial manner. Combined with the gradient pore size and density design of the molecular sieve filling structure, compared with the messy layout of each module and the single separation structure of the equipment in traditional technology, it can realize the orderly flow of materials and the graded interception of impurities, effectively improve the separation purity of tumor vaccine materials, and reduce the loss of target virus activity, which is more suitable for the core needs of tumor vaccine production.

[0019] 2. This solution uses an adaptive sealing linkage structure in conjunction with a flow guiding structure. Compared with traditional technologies, which suffer from poor equipment sealing, easy material deviation, and uneven separation, this solution can achieve rapid positioning and reliable sealing of the filling frame. At the same time, it guides the material to flow evenly and vertically, avoiding material leakage and deviation, enhancing the flow continuity between each filling layer, and further ensuring the stability of the separation effect.

[0020] 3. This solution links the control system with the flow regulation component, temperature sensor, early warning module, and data storage module. Compared with traditional technologies where equipment is mostly manually adjusted, abnormalities are difficult to detect in a timely manner, and data cannot be traced, this solution can achieve automatic control of feed pressure and separation temperature, timely early warning of abnormal situations during operation, synchronous recording of equipment operation and separation process data, simplifying the operation process, improving the safety and reliability of equipment operation, and meeting the rigorous requirements of basic research.

[0021] 4. This solution integrates a detachable filling frame, support feet, and filter components. Compared with traditional technologies, which involve cumbersome equipment maintenance, large operating vibrations, and incomplete impurity interception, this solution facilitates equipment cleaning, maintenance, and molecular sieve replacement. It also reduces vibrations generated during equipment operation, avoiding the impact of vibrations on separation efficiency. Furthermore, secondary filtration further intercepts impurities, improving the quality of purified materials, extending equipment lifespan, and reducing long-term operating costs. Attached Figure Description

[0022] Figure 1 This is an isometric view of an embodiment of the tumor vaccine manufacturing equipment of the present invention;

[0023] Figure 2 This is a side view of an embodiment of the tumor vaccine manufacturing equipment of the present invention;

[0024] Figure 3 for Figure 2 A cross-sectional view along the AA direction.

[0025] The reference numerals in the accompanying drawings of the instruction manual include: 1. Frame; 2. Feeding module; 201. Feeding funnel; 202. Feeding pump; 203. Feeding pipe; 3. Separation module; 301. Housing; 302. Feeding interface; 303. Discharge interface; 304. Observation window; 305. Temperature display panel; 4. Primary collection tank; 401. Sealed sampling valve; 402. Discharge valve; 5. Filling frame; 501. Divider plate; 502. Filling layer; 6. Support leg; 7. Touch screen. Detailed Implementation

[0026] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0028] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0029] The following detailed description illustrates the specific implementation method:

[0030] Example 1:

[0031] As attached Figures 1 to 3 As shown: A tumor vaccine manufacturing device includes a frame 1. A feeding module 2, a separation module 3, a collection module, and a control system are mounted on the frame 1. The control system coordinates the operation parameters of each module. The feeding module 2, separation module 3, and collection module are installed in layers from top to bottom along the height direction of the frame 1 (i.e., the feeding module 2 is fixedly connected to the top of the frame 1, the separation module 3 is detachably installed in the middle of the frame 1 and located directly below the feeding module 2, and the collection module is fixedly connected to the bottom of the frame 1 and located directly below the separation module 3). The feeding module 2, separation module 3, and collection module are arranged coaxially parallel to the axis of the frame 1. The control system is electrically connected to the feeding module 2, separation module 3, and collection module respectively. The separation module 3 includes a housing 301, inside which is a molecular sieve filling structure. The molecular sieve filling structure includes several filling layers 502. The molecular sieve pore size of each filling layer 502 is gradient-distributed, gradually decreasing from the feeding end to the discharging end, and the molecular sieve filling density of each filling layer 502 is negatively correlated with the pore size.

[0032] The top of the housing 301 of the separation module 3 is provided with a feed inlet 302 and the bottom of the housing 301 is provided with a discharge inlet 303. The feed inlet 302 is coaxially and sealed with the discharge end of the feed module 2, and the discharge inlet 303 is coaxially and sealed with the feed end of the collection module. The molecular sieve filling structure also includes a filling frame 5, which is detachably connected to the inner wall of the housing 301. The filling frame 5 is provided with several partition plates 501, which divide the filling frame 5 into several filling cavities. Each filling cavity corresponds to a filling layer 502. The side wall of the filling cavity is provided with several vent holes. The diameter of the vent holes is smaller than the molecular sieve pore diameter of the corresponding filling layer 502. The top of the filling frame 5 is provided with a guide port corresponding to the feed inlet 302 of the housing 301.

[0033] The inner wall of the housing 301 is provided with a ring-shaped heating layer and several uniformly distributed temperature sensors. The temperature sensors are electrically connected to the control system. The heating layer is used to maintain a constant temperature in the separation environment inside the housing 301. The control system is used to adjust the heating layer according to the real-time temperature monitoring of the temperature sensors. The side wall of the housing 301 is provided with an observation window 304 and a temperature display panel 305 electrically connected to the control system. The observation window 304 is used to observe the usage status of the filling layer 502 in the filling frame 5 in real time. The temperature display panel 305 is used to intuitively display the real-time temperature inside the housing 301.

[0034] The feeding module 2 includes a feeding funnel 201, a feeding pump 202, a feeding pipe 203, and a flow regulating component. The feeding funnel 201 is fixedly installed on the top of the frame 1. The outlet of the feeding funnel 201 is sealed to the inlet of the feeding pump 202. The feeding pump 202 is fixedly connected to the top side of the frame 1. The outlet of the feeding pump 202 is coaxially sealed to the inlet of the housing 301 through the feeding pipe 203. The flow regulating component includes a flow regulating valve and a pressure sensor. Both the flow regulating valve and the pressure sensor are installed on the feeding pipe 203 and electrically connected to the control system. The pressure sensor is used to detect the material pressure in the feeding pipe 203. The control system automatically adjusts the opening of the flow regulating valve according to the pressure feedback signal.

[0035] The collection module includes a primary collection tank 4, a filter assembly, a sampling port, and a discharge valve 402. The primary collection tank 4 is fixedly connected to the bottom of the frame 1, and the top inlet and outlet interface 303 of the primary collection tank 4 are coaxially and sealed. The filter assembly is embedded in the inlet of the primary collection tank 4. The filter assembly adopts a double-layer microporous membrane structure, and the pore size of the filter membrane is smaller than the molecular sieve particle size of the bottom filling layer 502. The filter assembly is used to intercept incompletely separated target particles and impurities. The sampling port is located in the middle of the side wall of the primary collection tank 4. A sealed sampling valve 401 is provided on the sampling port. The sealed sampling valve 401 is used for real-time sampling by the experimenter to detect the concentration and activity of the material. The discharge valve 402 is located at the bottom of the primary collection tank 4. The discharge valve 402 is used to discharge the purified material.

[0036] It also includes several support feet 6, which are fixedly connected to the bottom of the frame 1. The support feet 6 are used to reduce the vibration generated by the feed pump 202 during the operation of the equipment. The bottom of the support feet 6 is provided with anti-slip and wear-resistant pads.

[0037] The control system is electrically connected to a touch screen 7 and a data storage module. The touch screen 7 is used for parameter setting and real-time monitoring of the operating status, while the data storage module is used to store the equipment's operating parameters and separation process data.

[0038] The specific implementation process is as follows: First, the filling frame 5 of the separation module 3 is removed from the inside of the shell 301. According to the purification requirements of the tumor vaccine, molecular sieves of different pore sizes and densities are filled into each filling cavity of the filling frame 5. Following the principle of "gradually decreasing pore size and gradually increasing filling density from the feed end to the discharge end", the corresponding specification molecular sieve filling layer 502 is filled into each filling cavity in sequence to ensure that the molecular sieves are filled evenly without looseness or gaps. After filling, the filling frame 5 is installed on the inner wall of the shell 301, ensuring that the filling frame 5 is installed firmly and that the guide port at the top of the filling frame 5 is precisely aligned with the feed interface 302 at the top of the shell 301 to ensure smooth subsequent material flow. At the same time, the coaxial correspondence of the feeding module 2, separation module 3, and collection module is checked to ensure that the feeding pipe 203 and the feed interface 302 of the shell 301, and the discharge interface 303 of the shell 301 and the inlet of the primary collection tank 4 are coaxially sealed and connected to avoid material leakage. Ensure that the anti-slip and wear-resistant pads at the bottom of the support feet 6 are in close contact with the placement platform, so that the equipment is placed stably without shaking. Utilize the shock absorption effect of the support feet 6 to reduce the vibration that may be generated when the subsequent feed pump 202 is running, thus laying the foundation for the stability of the separation process.

[0039] The control system is then activated, and parameters are set via the touch screen 7. Based on the characteristics of the tumor vaccine material to be purified, a constant temperature is set for the separation environment inside the housing 301 (to meet the requirements for preserving viral activity). The normal pressure threshold inside the feed pipe 203 is also set. At the same time, the monitoring functions of the temperature sensor and pressure sensor are activated to ensure that the sensors and the control system work together normally. The temperature display panel 305 can display the temperature inside the housing 301 in real time, and the touch screen 7 can clearly display the operating status of each module.

[0040] After preliminary preparation and debugging, the purification of tumor vaccine materials begins. The tumor vaccine material to be separated and purified is slowly poured into the feed funnel 201. The material enters the feed pump 202 through the outlet of the feed funnel 201. The feed pump 202 is started, pressurizing the material and then conveying it to the separation module 3 through the feed pipe 203. During this process, a pressure sensor monitors the material pressure in the feed pipe 203 in real time and feeds the pressure signal back to the control system. When the detected pressure exceeds a preset threshold, the control system automatically adjusts the opening of the flow regulating valve to reduce the material conveying speed and lower the pressure in the pipe. When the pressure is below the preset threshold, the control system synchronously adjusts the flow regulating valve to increase the opening and increase the feeding speed, ensuring that the feeding pressure remains stable within the preset range. This ensures a smooth separation process and avoids pressure fluctuations affecting the separation effect and equipment.

[0041] The material enters the feed inlet 302 of the housing 301 of the separation module 3 through the feed pipe 203, and then enters the interior of the filling frame 5 through the guide port at the top of the filling frame 5. Under the action of gravity, the material flows from top to bottom through the molecular sieves of each filling layer 502. Since the pore size of the molecular sieves in each filling layer 502 is gradient distributed, and the filling density is negatively correlated with the pore size, the large-pore, low-density molecular sieve at the feed end can first intercept larger impurities (such as cell debris and large polymer molecules) in the material, the middle layer molecular sieves intercept medium-sized impurities (such as miscellaneous proteins), and the small-pore, high-density molecular sieve at the discharge end precisely intercepts small molecule impurities, while allowing target tumor virus particles to pass through, thus achieving efficient separation of impurities and target viruses.

[0042] During the separation process, the heating layer on the inner wall of the shell 301 works continuously. The temperature sensor monitors the temperature inside the shell 301 in real time and feeds the temperature data back to the control system. When the temperature deviates from the set value, the control system automatically adjusts the heating power of the heating layer to stabilize the temperature inside the shell 301 within the set range, with temperature fluctuations controlled within ±0.5℃. Operators can observe the usage status of the molecular sieve filling layer 502 in the filling frame 5 in real time through the observation window 304 on the side wall of the shell 301 to check for molecular sieve blockage, material deviation, etc. The real-time temperature inside the shell 301 can be intuitively monitored through the temperature display panel 305, which facilitates timely detection and handling of abnormalities.

[0043] The purified material from the separation module 3 flows into the primary collection tank 4 of the collection module through the discharge port 303 at the bottom of the shell 301. The material first passes through a double-layer microporous membrane filter assembly embedded at the inlet of the primary collection tank 4. The pore size of the membrane is smaller than the molecular sieve particle size of the bottom filling layer 502, effectively intercepting incompletely separated molecular sieve particles and small amounts of residual impurities, further improving the purity of the material. Operators can periodically take real-time samples through the sealed sampling valve 401 on the side wall of the primary collection tank 4 to detect the concentration and activity of the virus in the material. After sampling, the sealed sampling valve 401 is closed to ensure a sterile environment inside the collection tank and prevent material contamination.

[0044] During equipment operation, the support feet 6 continuously dampen vibrations, effectively reducing the vibrations generated by the feed pump 202. This prevents the molecular sieve filling layer 502 within the filling frame 5 from loosening or causing material flow turbulence, thus ensuring separation accuracy. Simultaneously, it prevents vibration from causing misalignment of the coaxial connections between modules or seal failure, improving the stability of equipment operation. The control system's storage module synchronously records equipment operating parameters (such as feed pressure, temperature inside the shell 301, and flow control valve opening) and separation process data, facilitating the traceability, analysis, and replication of subsequent experimental data, meeting the needs of basic research.

[0045] When the sample detects that the virus concentration and activity in the material reach the preset standard, the feed pump 202 is turned off to stop feeding. After all the remaining material in the separation module 3 flows into the primary collection tank 4, the discharge valve 402 at the bottom of the primary collection tank 4 is opened to discharge the purified tumor vaccine material, completing one purification operation. After the operation is completed, the control system is turned off, the filling frame 5 of the separation module 3 is disassembled, and the filling frame 5, each filling layer 502 molecular sieve, the feed funnel 201, the feed pipe 203, the primary collection tank 4, and the filter components are cleaned and disinfected for future use.

[0046] Example 2:

[0047] The difference from Embodiment 1 is that a sealing linkage structure is provided between the housing 301 and the filling frame 5. The sealing linkage structure includes an annular sealing groove, an elastic sealing ring, and a limiting boss. The annular sealing groove is opened on the inner wall of the housing 301, the elastic sealing ring is embedded in the annular sealing groove, and the limiting boss is fixedly connected to the outer wall of the filling frame 5. When the filling frame 5 is installed into the housing 301, the limiting boss engages with the edge of the annular sealing groove for positioning, and at the same time, it squeezes the elastic sealing ring, so that the elastic sealing ring is tightly fitted with the outer wall of the filling frame 5 and the inner wall of the annular sealing groove, forming a double coaxial seal.

[0048] The specific implementation process is as follows: The filling frame 5, filled with molecular sieve, is then aligned with the inner cavity of the shell 301, ensuring the limiting boss on the outer wall of the filling frame 5 is aligned with the edge of the annular sealing groove. The filling frame 5 is then slowly installed into the shell 301. During the installation process, the limiting boss precisely engages with the edge of the annular sealing groove, achieving rapid positioning of the filling frame 5 and the shell 301 without the need for additional positioning tools. Simultaneously, the limiting boss compresses the elastic sealing ring, causing it to elastically deform and tightly adhere to the inner wall of the annular sealing groove and the outer wall of the filling frame 5, forming a double coaxial seal. After assembly, the sealing fit is checked to ensure there are no gaps or looseness, guaranteeing a good sealing effect.

[0049] During equipment operation, the sealing linkage structure continues to function, and the double seal effectively prevents material leakage while isolating outside air from entering the housing 301. Combined with the heating layer, it maintains a constant temperature environment within the housing 301, preventing temperature fluctuations and bacterial contamination. When disassembling the filling frame 5, pulling it upwards causes the limiting boss to disengage from the annular sealing groove, and the elastic sealing ring automatically resets.

[0050] Example 3:

[0051] The difference from Embodiment 2 is that the partition plate 501 is provided with a plurality of guide holes corresponding to the vent holes. The diameter of the guide holes is the same as the diameter of the vent holes of the corresponding filling cavity, and the guide holes and the vent holes are arranged coaxially. The partition plate 501 and the interior of the filling cavity form an annular guide step. The guide step is connected to the guide port at the top of the filling frame 5. The guide step is used to realize that after the material enters from the guide port, it is diverted to each filling cavity through the guide step. At the same time, the coaxial linkage between the guide holes of the hollow partition plate 501 and the vent holes of the filling cavity ensures vertical flow of the material, avoids uneven separation caused by material deviation, and enhances the continuity of material flow between each filling layer 502.

[0052] The specific implementation process is as follows: First, check the guide holes on the partition plate 501 to confirm that the guide holes correspond one-to-one with the ventilation holes on the side walls and bottom of each filling cavity, that the hole diameters are completely consistent and arranged coaxially, and that there is no blockage or offset, so as to ensure smooth material flow.

[0053] After each filling chamber of the filling frame 5 is filled with molecular sieves of the corresponding specifications, the filling frame 5 is installed into the housing 301 of the separation module 3 and fixed, ensuring that the annular guide step is tightly connected to the guide port at the top of the filling frame 5, and that the guide hole and the vent hole are coaxially aligned. After the equipment is started, the material enters the guide port of the filling frame 5 through the feed interface 302 of the housing 301, and is evenly distributed to each filling chamber along the annular guide step, avoiding the material from concentrating in a single filling chamber.

[0054] Under the influence of gravity, the material permeates downwards and flows vertically into the lower filling cavity through the guide holes of the separator 501. This forms a coaxial linkage with the venting holes, ensuring that the material always flows vertically without deviation or stagnation. The material in each filling layer 502 achieves continuous flow through the precise connection between the guide holes and the venting holes, ensuring full contact between the material and each layer of molecular sieves to complete the classification and separation.

[0055] Example 4:

[0056] The difference from Embodiment 3 is that it also includes an early warning module. The early warning module is used to provide real-time warnings for abnormal situations during operation. The early warning module includes an indicator light and a buzzer. Both the indicator light and the buzzer are electrically connected to the control system. When the pressure in the feed pipe 203 exceeds the preset pressure threshold in the control system and the temperature in the housing 301 deviates from the set temperature threshold range, the control system triggers the indicator light to emit a warning light of the corresponding color and the buzzer to emit an intermittent alarm sound. At the same time, the control system records the abnormal information synchronously.

[0057] The specific implementation process is as follows: Enter the control system parameter setting interface through the touch operation screen 7, preset the pressure threshold of the feed pipe 203 and the temperature threshold inside the shell 301, set the early warning trigger logic, and ensure that the pressure sensor, temperature sensor and early warning module are linked.

[0058] After the equipment is started, the early warning module goes into standby mode synchronously with the control system. The pressure sensor monitors the pressure inside the feed pipe 203 in real time, and the temperature sensor monitors the temperature inside the housing 301 in real time. Both transmit the real-time data synchronously to the control system. When the pressure inside the feed pipe 203 exceeds the preset pressure threshold, or the temperature inside the housing 301 deviates from the set temperature threshold range, the control system immediately triggers the early warning module.

[0059] At this time, the indicator light emits a corresponding color warning light (red for abnormal pressure, yellow for abnormal temperature), and the buzzer emits an intermittent alarm sound to remind the operator to check for abnormalities in a timely manner. Simultaneously, the control system records abnormal information through the data storage module, including the abnormality type, trigger time, and real-time pressure / temperature data, facilitating subsequent traceability and analysis. After the operator has checked and resolved the abnormality (such as adjusting the feed speed or checking the heating layer), the abnormality is resolved, the warning module automatically stops working, and the system returns to standby mode.

[0060] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A tumor vaccine manufacturing device, comprising a frame (1), on which a feeding module (2), a separation module (3), a collection module, and a control system are mounted, the control system being used to coordinate the control of the operating parameters of each module, characterized in that, The feeding module (2), separation module (3) and collection module are installed in layers from top to bottom along the height direction of the frame (1). The feeding module (2), separation module (3) and collection module are arranged coaxially parallel to the axis of the frame (1). The control system is electrically connected to the feeding module (2), separation module (3) and collection module respectively. The separation module (3) includes a housing (301), and a molecular sieve filling structure is provided inside the housing (301). The molecular sieve filling structure includes several filling layers (502). The molecular sieve pore size of each filling layer (502) is distributed in a gradient. The molecular sieve pore size gradually decreases from the feed end to the discharge end, and the molecular sieve filling density of each filling layer (502) is negatively correlated with the pore size.

2. The tumor vaccine manufacturing equipment according to claim 1, characterized in that, The top of the housing (301) of the separation module (3) is provided with a feed port (302), and the bottom of the housing (301) is provided with a discharge port (303). The feed port (302) is coaxially and sealed with the discharge end of the feed module (2), and the discharge port (303) is coaxially and sealed with the feed end of the collection module. The molecular sieve filling structure also includes a filling frame (5). The filling frame (5) is detachably connected to the inner wall of the housing (301). The filling frame (5) is provided with several partition plates (501). The partition plates (501) divide the filling frame (5) into several filling cavities. Each filling cavity corresponds to a filling layer (502). The side wall of the filling cavity is provided with several air vents. The diameter of the air vents is smaller than the molecular sieve pore diameter of the corresponding filling layer (502). The top of the filling frame (5) is provided with a guide port corresponding to the feed port (302) of the housing (301).

3. The tumor vaccine manufacturing equipment according to claim 2, characterized in that, The inner wall of the housing (301) is provided with a ring-shaped heating layer and several uniformly distributed temperature sensors. The temperature sensors are electrically connected to the control system. The heating layer is used to maintain a constant temperature of the separation environment inside the housing (301). The control system is used to adjust the heating layer according to the real-time temperature monitored by the temperature sensors. The side wall of the housing (301) is provided with an observation window (304) and a temperature display panel (305) electrically connected to the control system. The observation window (304) is used to observe the usage status of the filling layer (502) in the filling frame (5) in real time. The temperature display panel (305) is used to intuitively display the real-time temperature inside the housing (301).

4. The tumor vaccine manufacturing equipment according to claim 3, characterized in that, The feeding module (2) includes a feeding funnel (201), a feeding pump (202), a feeding pipe (203), and a flow regulating component. The feeding funnel (201) is fixedly installed on the top of the frame (1). The outlet of the feeding funnel (201) is sealed to the inlet of the feeding pump (202). The feeding pump (202) is fixedly connected to the top side of the frame (1). The outlet of the feeding pump (202) is coaxially sealed to the inlet of the housing (301) through the feeding pipe (203). The flow regulating component includes a flow regulating valve and a pressure sensor. Both the flow regulating valve and the pressure sensor are installed on the feeding pipe (203) and electrically connected to the control system. The pressure sensor is used to detect the material pressure in the feeding pipe (203). The control system automatically adjusts the opening of the flow regulating valve according to the pressure feedback signal.

5. The tumor vaccine manufacturing equipment according to claim 4, characterized in that, The collection module includes a primary collection tank (4), a filter assembly, a sampling port, and a discharge valve (402). The primary collection tank (4) is fixedly connected to the bottom of the frame (1). The top inlet and outlet interface (303) of the primary collection tank (4) are coaxially and sealed. The filter assembly is embedded in the inlet of the primary collection tank (4). The filter assembly is used to intercept incompletely separated target particles and impurities. The sampling port is located in the middle of the side wall of the primary collection tank (4). A sealed sampling valve (401) is provided on the sampling port. The sealed sampling valve (401) is used for real-time sampling by the experimenter to detect the concentration and activity of the material. The discharge valve (402) is located at the bottom of the primary collection tank (4). The discharge valve (402) is used to discharge the purified material.

6. The tumor vaccine manufacturing equipment according to claim 5, characterized in that, A sealing linkage structure is provided between the housing (301) and the filling frame (5). The sealing linkage structure includes an annular sealing groove, an elastic sealing ring and a limiting boss. The annular sealing groove is opened on the inner wall of the housing (301), the elastic sealing ring is embedded in the annular sealing groove, and the limiting boss is fixed to the outer wall of the filling frame (5).

7. The tumor vaccine manufacturing equipment according to claim 6, characterized in that, The partition plate (501) is provided with several guide holes corresponding to the vent holes. The diameter of the guide holes is consistent with the diameter of the vent holes of the corresponding filling cavity, and the guide holes and vent holes are arranged coaxially. The partition plate (501) and the interior of the filling cavity form an annular guide step. The guide step is connected to the guide port at the top of the filling frame (5). The guide step is used to realize that the material enters from the guide port and is diverted to each filling cavity through the guide step.

8. The tumor vaccine manufacturing equipment according to claim 7, characterized in that, It also includes several support feet (6), which are fixedly connected to the bottom of the frame (1). The support feet (6) are used to reduce the vibration generated by the feed pump (202) during the operation of the equipment. The bottom of the support feet (6) is provided with anti-slip and wear-resistant pads.

9. The tumor vaccine manufacturing equipment according to claim 8, characterized in that, It also includes an early warning module, which is used to provide real-time warnings for abnormal situations during operation. The early warning module includes an indicator light and a buzzer. Both the indicator light and the buzzer are electrically connected to the control system. When the pressure in the feed pipe (203) exceeds the preset pressure threshold in the control system and the temperature in the shell (301) deviates from the set temperature threshold range, the control system triggers the indicator light to emit a warning light of the corresponding color and the buzzer to emit an intermittent alarm sound. At the same time, the control system records the abnormal information synchronously.

10. The tumor vaccine manufacturing equipment according to claim 9, characterized in that, The control system is electrically connected to a touch screen (7) and a data storage module. The touch screen (7) is used for parameter setting and real-time monitoring of the operating status, and the data storage module is used to store the equipment operating parameters and separation process data.

Images