Adjustable pathological sample automatic dehydration and wax immersion integrated machine

Abstract

The application discloses a kind of adjustable pathological sample automation dehydration and wax immersion integrated machine, it is related to pathological sample processing technical field, including frame, rear side is fixedly connected with vertical plate on the top of frame, installation beam is fixedly connected with the top of front side of vertical plate, transfer assembly is installed in the bottom of installation beam, dehydration assembly is arranged at the middle position in the inside of frame;Wherein, dehydration assembly includes shell, shell is fixedly connected at the middle position in the inside of frame, at least six blocking strips are fixedly connected with the bottom surface in the inside of shell, adjacent two blocking strips are slidably connected with solvent storage tank.Occupational advantage is in: by integrating "fully sealed solvent storage and delivery system", "the intelligent fluid control system that can be automatically cleaned and prevents backflow" and "dehydration cylinder dynamic sealing mechanism", the inherent solvent cross-contamination and volatile problem of traditional multi-cylinder dehydration machine is systematically solved.

Description

Technical Field

[0001] This invention relates to the field of pathological sample processing technology, specifically to an adjustable automated dehydration and paraffin impregnation machine for pathological samples. Background Technology

[0002] With the development of precision medicine, the role of pathological diagnosis is becoming increasingly prominent, and it is bound to become a powerful force in medical advancement. The accuracy of pathological diagnosis depends on the quality of pathological slides; the quality of the slides can even affect the diagnostic results. Therefore, slide quality has become a fundamental standard for pathological quality control. Currently, new technologies, products, and equipment in pathology are constantly emerging, driving the continuous progress of the discipline and gradually moving it towards automation, standardization, and intelligence.

[0003] For example, Chinese Patent (CN214538888U) discloses an automated tissue dehydration and embedding machine for the fully automated process of preparing paraffin blocks for biological tissue samples. Its main feature is the use of a specimen basket that can extend and retract vertically on both sides. When the basket moves between several V-shaped bottom reagent cylinders and a final flat-bottom reagent cylinder, it is in a narrowed, extended state within the V-shaped bottom cylinder. When the basket reaches the final flat-bottom reagent cylinder, its own weight causes it to fall, changing from a narrowed to an extended state, as the flat-bottom cylinder is deeper than the V-shaped bottom cylinder. The extended basket is then immersed in the final flat-bottom reagent cylinder containing molten liquid paraffin. When the basket is lifted out of the cylinder, the embedding cassette inside is filled with liquid paraffin. After cooling, the paraffin solidifies, embedding the tissue specimen within the cassette. This technology is widely used in clinical pathology, cytology, and biological research.

[0004] In existing technologies, multiple tanks are used to simultaneously dehydrate several groups of biological tissue samples. However, this method involves a mechanical arm immersing a specimen basket into V-bottom reagent tanks containing different concentrations of ethanol. After dehydration at a low concentration, the sample is transferred to the next higher concentration ethanol solution by the mechanical arm. The sample inevitably retains some of the previous low-concentration ethanol solution, which is then diluted over time, affecting the dehydration efficiency. Furthermore, the existing V-bottom reagent tanks are open, and since ethanol is volatile, the ethanol concentration gradually decreases. This concentration change can lead to uneven dehydration, affecting the dehydration effect and potentially causing incomplete or excessive dehydration in certain areas. Therefore, this invention proposes an adjustable automated dehydration and paraffin infusion machine for pathological samples. Summary of the Invention

[0005] The purpose of this invention is to provide an adjustable automated dehydration and paraffin infusion machine for pathological samples, to solve the problem of using multiple cylinders to simultaneously dehydrate several groups of biological tissue samples as described in the background art. However, the above method involves a mechanical arm immersing a specimen basket into V-bottom reagent cylinders containing ethanol of different concentrations. After dehydration at a low concentration, the sample is transferred to the next high-concentration ethanol solution by the mechanical arm. The sample will inevitably carry some of the previous low-concentration ethanol solution, which will then enter the next high-concentration ethanol solution. Over time, the high-concentration ethanol solution will be diluted, affecting the dehydration efficiency of the sample. In addition, the existing V-bottom reagent cylinders are all open, and since ethanol is volatile, the ethanol concentration in the container gradually decreases. With the change in concentration, the dehydration process may be uneven, affecting the dehydration effect of the tissue sample, and may even lead to incomplete or excessive dehydration in some areas.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] An adjustable automated dehydration and paraffin-impregnation machine for pathological samples includes a frame, a vertical plate fixedly connected to the rear side of the frame, an installation beam fixedly connected to the upper front side of the vertical plate, a transfer component installed at the bottom of the installation beam, and a dehydration component located in the middle of the inside of the frame.

[0008] The dehydration assembly includes a housing, which is fixedly connected to the middle of the machine frame. At least six baffles are fixedly connected to the bottom surface of the housing. A solvent storage tank is slidably connected between two adjacent baffles. The rear side wall of the housing is fixedly connected with the same number of second seals as the solvent storage tanks at the corresponding positions. Each of the five solvent storage tanks is fixedly connected with a first seal, which is inserted into the second seal. A suction tube is fixedly connected to the front of the first seal inside the solvent storage tank. Each of the five second seals is fixedly connected with a single-way solenoid valve via a flange.

[0009] Optionally, a bracket for placing embedding boxes is fixedly connected to the upper left side of the frame, and two sets of moving guide rails are fixedly connected to the upper right side of the frame. A placement plate is slidably connected to the two sets of moving guide rails via sliding blocks. A paraffin embedding machine is fixedly installed on the upper side of the frame behind the moving guide rails. An electric push rod is fixedly connected to the upper side of the frame in front of the moving guide rails. A connecting piece is fixedly connected to the output end of the electric push rod. The rear side of the connecting piece is fixedly connected to the front side of the placement plate. A positioning contact is fixedly connected between the two sets of moving guide rails on the upper right side of the frame.

[0010] Optionally, a partition is fixedly connected to the middle position inside the outer shell, and a wastewater tank is detachably connected above the partition. A dehydration cylinder is fixedly connected to the top wall inside the outer shell. A drain pipe with a valve is installed at the bottom of the dehydration cylinder. The drain pipe is located directly above the inlet of the wastewater tank. An inspection door is rotatably connected to the front side of the outer shell via a hinge. A spring compression member with the same number as the solvent storage tank is fixedly connected to the lower inner side of the inspection door. An observation window for observation is provided on the front side of the inspection door.

[0011] Optionally, a merging box is fixedly connected to the middle of the rear side of the outer casing. The bottom of the merging box is fixedly connected to the same number of connecting hoses as the solvent storage tank. The ends of the five sets of connecting hoses are respectively connected to five single-way solenoid valves. A connecting plate is fixedly connected to the upper rear side inside the outer casing. An acid and alkali resistant centrifugal pump is fixedly connected to the upper part of the connecting plate. An inlet pipe is fixedly connected to the input end of the acid and alkali resistant centrifugal pump. The end of the inlet pipe passes through the rear side wall of the outer casing and is fixedly connected to the upper part of the merging box. A drain pipe is fixedly connected to the output end of the acid and alkali resistant centrifugal pump. The end of the drain pipe is connected to the inside of the dehydration tank.

[0012] Optionally, a fixing plate is fixedly connected to the upper left and right sides of the outer shell, and a bidirectional lead screw is rotatably connected between the two fixing plates at the rear. The two ends of the outer surface of the bidirectional lead screw are threaded with a third threaded seat. A guide rod is fixedly connected to the front side between the two fixing plates. The two ends of the outer surface of the guide rod are slidably connected with a sliding member. A cover plate is fixedly connected between the third threaded seat and the sliding member on both sides. A servo motor for driving the bidirectional lead screw to rotate is fixedly installed on the side of the left fixing plate.

[0013] Optionally, the transfer assembly includes two fixed frames, which are respectively fixedly connected to the left and right sides of the bottom of the mounting beam. A first threaded rod is rotatably connected inside each of the two fixed frames. A first limiting rod is fixedly connected below the first threaded rod inside each of the two fixed frames. A first threaded seat is threadedly connected to the outer surface of each of the two first threaded rods. The first threaded seat and the first limiting rod are slidably connected through a guide sleeve. A first motor for driving the first threaded rod to rotate is fixedly connected to the front side of each of the two fixed frames.

[0014] Optionally, a connecting seat is fixedly connected to the bottom of each of the two first threaded seats, and a second threaded rod is rotatably connected between the two connecting seats on the rear side. A second threaded seat is threadedly connected to the outer surface of the second threaded rod, and a second limiting rod is fixedly connected to the front side of the two connecting seats. A sliding seat is slidably connected to the outer surface of the second limiting rod, and the rear side of the sliding seat is fixedly connected to the front side of the second threaded seat. A second motor for driving the second threaded rod to rotate is fixedly connected to at least one side of the connecting seat.

[0015] Optionally, a fixed seat is fixedly connected to the front side of the sliding seat, and a lifting cylinder is fixedly connected above the fixed seat. The output end of the lifting cylinder passes through the bottom surface of the fixed seat and is fixedly connected to a connecting strip. A pneumatic suction cup for adsorbing the embedding box is fixedly connected to the bottom of the connecting strip.

[0016] Optionally, the frame is also equipped with a controller and an air supply unit for air supply, and omnidirectional casters for moving the device are fixedly connected to the four corners of the bottom of the frame.

[0017] The beneficial effects of this invention are:

[0018] 1. This invention adopts a structure of "one dehydration tank combined with five independent solvent storage boxes". The sample is always dehydrated in the same dehydration tank, avoiding the carrying of residual solvent when the sample is transferred between different tanks. Through the coordinated control of a single-way solenoid valve and an acid and alkali resistant centrifugal pump, the residual solvent of the previous concentration in the pipeline can be emptied before each solvent switch. The one-way valve in the connecting hose further blocks the solvent backflow, eliminating cross-contamination of solvents of different concentrations from the source, ensuring that the high-concentration solvent is always maintained at the preset concentration, and improving the dehydration efficiency. The solvent storage box is precisely connected to the second sealing part of the outer shell through the first sealing part, and with the single-way solenoid valve fixed by the flange, a fully sealed solvent transmission channel is formed. This not only prevents solvent leakage and contamination of the equipment interior, but also prevents solvents in different storage boxes from mixing through gaps, further ensuring the purity and stability of solvents of various concentrations.

[0019] 2. In this invention, the first seal on the rear side of the solvent storage tank is inserted into the second seal on the outer shell to ensure no leakage during solvent delivery; the cover plate above the dehydration tank is opened and closed by a servo motor-driven bidirectional screw. When soaking, the cover plate is closed to prevent solvent evaporation, protecting the health of operators and reducing solvent loss; a single-way solenoid valve precisely controls the solvent flow, avoiding cross-contamination of solvents of different concentrations; the drain pipe at the bottom of the dehydration tank is aligned with the wastewater tank, allowing waste solvent after dehydration to be directly discharged into the wastewater tank for centralized treatment, avoiding environmental pollution caused by indiscriminate solvent discharge; the wastewater tank is detachable and can be quickly replaced when full, making operation convenient and compliant with laboratory environmental protection regulations. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0021] Figure 1 This is a schematic diagram of the structure of the present invention;

[0022] Figure 2This is a schematic diagram of the structure from another perspective of the present invention;

[0023] Figure 3 This is a schematic diagram of the transfer component structure in this invention;

[0024] Figure 4 This is a schematic diagram of the transfer component in this invention from another perspective;

[0025] Figure 5 This is a schematic diagram of the dehydration component structure in this invention;

[0026] Figure 6 This is a schematic diagram of the structure for removing the maintenance door of the dehydration component in this invention;

[0027] Figure 7 This is a schematic diagram of the internal structure of the dehydration component in this invention;

[0028] Figure 8 This is a schematic diagram showing the connection between the solvent storage tank and the confluence tank in this invention;

[0029] Figure 9 This is a cross-sectional view of the solvent storage tank in this invention;

[0030] Figure 10 for Figure 1 A magnified view of a section at point A in the middle;

[0031] Figure 11 for Figure 5 A magnified view of a section at point B in the middle;

[0032] Figure 12 for Figure 9 A magnified view of a section at point C.

[0033] The numbers on the map are:

[0034] 1. Frame; 2. Vertical plate; 3. Mounting beam;

[0035] 4. Transfer assembly; 401. Fixing frame; 402. First threaded rod; 403. First limiting rod; 404. First motor; 405. First threaded seat; 406. Connecting seat; 407. Second threaded rod; 408. Second limiting rod; 409. Second threaded seat; 410. Sliding seat; 411. Second motor; 412. Fixing seat; 413. Lifting cylinder; 414. Connecting bar; 415. Pneumatic suction cup;

[0036] 5. Bracket;

[0037] 6. Dehydration assembly; 601. Housing; 602. Inspection door; 603. Partition; 604. Baffle; 605. Solvent storage tank; 606. Suction tube; 607. First seal; 608. Second seal; 609. Single-way solenoid valve; 610. Merging box; 611. Connecting hose; 612. Connecting plate; 613. Acid and alkali resistant centrifugal pump; 614. Inlet pipe; 615. Drain pipe; 616. Dehydration cylinder; 617. Wastewater tank; 618. Fixing plate; 619. Guide rod; 620. Bidirectional lead screw; 621. Servo motor; 622. Third threaded seat; 623. Sliding part; 624. Cover plate; 625. Spring compression part;

[0038] 7. Moving guide rail; 8. Paraffin embedding machine; 9. Placement plate; 10. Electric push rod; 11. Connecting piece; 12. Positioning contact. Detailed Implementation

[0039] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0040] As attached Figure 1 To be continued Figure 12 As shown, the present invention provides an adjustable automated dehydration and paraffin impregnation machine for pathological samples, including a frame 1, a vertical plate 2 fixedly connected to the rear side of the frame 1, an installation beam 3 fixedly connected to the upper front side of the vertical plate 2, a transfer component 4 installed at the bottom of the installation beam 3, and a dehydration component 6 set in the middle position inside the frame 1. The transfer component 4 can realize the automated transfer of pathological samples, and the dehydration component 6 is responsible for sample dehydration treatment.

[0041] The dehydration component 6 includes a housing 601, which is fixedly connected to the middle of the frame 1. At least six baffles 604 are fixedly connected to the bottom surface of the housing 601. A solvent storage tank 605 is slidably connected between adjacent baffles 604. The six baffles 604 allow the solvent storage tanks 605 between adjacent baffles 604 to slide and pull out, facilitating solvent addition and tank replacement. A second sealing element 608, the same number as the solvent storage tanks 605, is fixedly connected to the rear wall of the housing 601 at a corresponding position on the solvent storage tank 605. A first sealing element 607 is fixedly connected to the rear side of each of the five solvent storage tanks 605, and the first sealing element 607 is inserted into the second sealing element 608. A suction tube 606 is fixedly connected to the front side of the first sealing element 607 inside the solvent storage tank 605. The five second sealing elements... Each of the 608 components is fixedly connected to a single-way solenoid valve 609 via a flange. The solvent storage tank 605 has five sets of containers, from left to right, containing 70% ethanol, 80% ethanol, 95% ethanol, anhydrous ethanol, and xylene. 70% ethanol: for initial dehydration, removing some water; 95% ethanol: for deep dehydration, serving as a transition for subsequent anhydrous ethanol treatment; anhydrous ethanol: to completely remove moisture from the sample, preventing water from not mixing with paraffin during subsequent paraffin impregnation; xylene: to make the sample transparent, improving the paraffin impregnation effect. The first seal 607 on the rear side of the solvent storage tank 605 is inserted into the corresponding second seal 608 on the rear wall of the outer shell 601, ensuring the airtightness of solvent transmission and preventing leakage and contamination. The suction tube 606 inside the solvent storage tank 605, in conjunction with the single-way solenoid valve 609 on the rear side, can precisely control the output of different solvents.

[0042] In one embodiment of the present invention, a bracket 5 for placing embedding cassettes is fixedly connected to the upper left side of the frame 1. The bracket 5 facilitates the centralized placement of embedding cassettes and makes it convenient for the transfer component 4 to grasp the samples. Two sets of moving guide rails 7 are fixedly connected to the upper right side of the frame 1. A placement plate 9 is slidably connected to the upper side of the two sets of moving guide rails 7 via sliding blocks. A paraffin embedding machine 8 is fixedly installed on the upper side of the frame 1 behind the moving guide rails 7. The paraffin embedding machine 8 can be an ATR-BML50 model. An electric push rod 10 is fixedly connected to the upper side of the frame 1 in front of the moving guide rails 7. A connecting piece 11 is fixedly connected to the output end of the electric push rod 10. The rear side of the connecting piece 11 is fixedly connected to the front side of the placement plate 9. A positioning contact 12 is fixedly connected between the two sets of moving guide rails 7 on the upper right side of the frame 1. The electric push rod 10 on the front side drives the placement plate 9 to move precisely along the moving guide rails 7 via the connecting piece 11. With the help of the positioning contact 12 on the upper right side of the frame 1, the placement plate 9 is ensured to stop accurately, which facilitates the paraffin embedding machine 8 to perform the paraffin embedding operation.

[0043] like Figure 5-9As shown in Figures 11-12, in one embodiment of the present invention, a partition 603 is fixedly connected to the middle position inside the outer shell 601. A wastewater tank 617 is detachably connected above the partition 603. A dehydration cylinder 616 is fixedly connected to the top wall inside the outer shell 601. A drain pipe with a valve is provided at the bottom of the dehydration cylinder 616. The drain pipe is located directly above the inlet of the wastewater tank 617 (the valve here is a solenoid valve, which can automatically open and close to achieve automated discharge of waste liquid). The front side of the outer shell 601 is rotatably connected to a hinge. The maintenance door 602 has spring compression members 625, the same number as those in the solvent storage tank 605, fixedly connected to its lower inner side. An observation window is provided on the front side of the maintenance door 602 for observation. The maintenance door 602 facilitates internal maintenance of the equipment. The spring compression members 625, the same number as those in the solvent storage tank 605, can tightly press the solvent storage tank 605 to ensure its stable installation. The observation window on the front side allows for real-time observation of the sample processing status in the dehydration tank 616, taking into account maintenance convenience, operational stability, and operational visibility.

[0044] In one embodiment of the present invention, a merging box 610 is fixedly connected to the middle of the rear side of the outer casing 601. A one-way valve (not shown) is also installed inside the connecting hose 611 near the merging box 610 to prevent ethanol solutions of different concentrations from entering other solvent storage tanks 605. This is prior art and will not be described in detail here. The bottom of the merging box 610 is fixedly connected to the same number of connecting hoses 611 as the solvent storage tanks 605. The ends of the five sets of connecting hoses 611 are respectively connected to five one-way solenoid valves 609. A connecting plate 612 is fixedly connected to the upper rear side inside the outer casing 601. An acid-alkali resistant centrifugal pump 613 is fixedly connected above the connecting plate 612. The input end of the acid-alkali resistant centrifugal pump 613 is fixedly connected to... A liquid inlet pipe 614 is fixedly connected to the merging tank 610. The end of the liquid inlet pipe 614 passes through the rear side wall of the outer shell 601 and is fixedly connected to the top of the merging tank 610. The output end of the acid and alkali resistant centrifugal pump 613 is fixedly connected to the drain pipe 615. The end of the drain pipe 615 is connected to the inside of the dehydration tank 616. The acid and alkali resistant centrifugal pump 613 draws solvent from the merging tank 610 through the liquid inlet pipe 614 and then delivers it to the dehydration tank 616 through the drain pipe 615. The controller controls the five single-way solenoid valves 609 to open sequentially from left to right. At the same time, the controller controls the drain pipe valve to discharge the upper concentration of ethanol to the wastewater tank 617, realizing the automated gradient dehydration of the sample without manual intervention. This ensures the precision and orderliness of the dehydration process and improves the processing efficiency.

[0045] In one embodiment of the present invention, fixing plates 618 are fixedly connected to the left and right sides of the outer shell 601. A bidirectional lead screw 620 is rotatably connected between the two fixing plates 618 on the rear side. The left and right ends of the outer surface of the bidirectional lead screw 620 are threaded with third thread seats 622. A guide rod 619 is fixedly connected to the front side between the two fixing plates 618. The left and right ends of the outer surface of the guide rod 619 are slidably connected with sliding members 623. A cover plate 624 is fixedly connected between the third thread seats 622 and the sliding members 623 on the left and right sides. A servo motor 621 for driving the bidirectional lead screw 620 to rotate is fixedly installed on the side of the left fixing plate 618. The servo motor 621 on the side of the left fixing plate 618 drives the bidirectional lead screw 620 to rotate, which can realize the synchronous opening and closing of the left and right cover plates 624. It can seal the dehydration cylinder 616 during the dehydration process to prevent external impurities from entering or solvent from evaporating, and can also automatically open during sample transfer. The servo motor 621 ensures that the opening and closing position is accurate, which improves the automation protection capability of the equipment.

[0046] like Figure 3-4 As shown, in one embodiment of the present invention, the transfer assembly 4 includes two fixed frames 401, which are respectively fixedly connected to the left and right sides of the bottom of the mounting beam 3. A first threaded rod 402 is rotatably connected inside each of the two fixed frames 401. A first limiting rod 403 is fixedly connected below the first threaded rod 402 inside each of the two fixed frames 401. A first threaded seat 405 is threadedly connected to the outer surface of each of the two first threaded rods 402. The first threaded seat 405 and the first limiting rod 403 are slidably connected through a guide sleeve. A first motor 404 for driving the first threaded rod 402 to rotate is fixedly connected to the front side of each of the two fixed frames 401. The first motor 404 drives the first threaded rod 402 to rotate, enabling the first threaded seat 405 to move smoothly and accurately in the front-back direction. The first limiting rod 403 effectively prevents the first threaded seat 405 from rotating, ensuring the stability and accuracy of the front-back movement of the transfer assembly 4, and providing a foundation for accurate sample transfer.

[0047] In one embodiment of the present invention, a connecting seat 406 is fixedly connected to the bottom of each of the two first threaded seats 405. A second threaded rod 407 is rotatably connected between the two connecting seats 406 at the rear. A second threaded seat 409 is threadedly connected to the outer surface of the second threaded rod 407. A second limiting rod 408 is fixedly connected to the front of the two connecting seats 406. A sliding seat 410 is slidably connected to the outer surface of the second limiting rod 408. The rear side of the sliding seat 410 is fixedly connected to the front side of the second threaded seat 409. A second motor 411 for driving the second threaded rod 407 to rotate is fixedly connected to the side of at least one connecting seat 406. The second motor 411 drives the second threaded rod 407 to rotate, so that the sliding seat 410 can move smoothly in the left and right direction. The second limiting rod 408 ensures that the sliding seat 410 does not rotate. In conjunction with the left and right direction movement, the two-dimensional planar movement of the transfer component 4 is realized, which improves the flexibility and accuracy of sample grasping and placement.

[0048] In one embodiment of the present invention, a fixed seat 412 is fixedly connected to the front side of the sliding seat 410, and a lifting cylinder 413 is fixedly connected above the fixed seat 412. The output end of the lifting cylinder 413 passes through the bottom surface of the fixed seat 412 and is fixedly connected to a connecting strip 414. A pneumatic suction cup 415 for adsorbing the embedding cassette is fixedly connected to the bottom of the connecting strip 414. The lifting cylinder 413 realizes the lifting action in the up and down direction. Combined with the two-dimensional planar movement, it forms a three-dimensional transfer system. The pneumatic suction cup 415 can stably grasp the embedding cassette and avoid damaging the sample. The lifting cylinder 413 is precisely controlled to ensure the smooth transfer of the sample between different workstations and improve the reliability of automated transfer.

[0049] In one embodiment of the present invention, the frame 1 is also equipped with a controller and an air supply component for air supply. The four corners of the bottom of the frame 1 are fixedly connected with universal casters for moving the device. The air supply component provides a stable air supply for pneumatic components such as the pneumatic suction cup 415, ensuring the normal operation of the pneumatic components. The universal casters at the four corners of the bottom of the frame 1 enable the equipment to move flexibly, making it easy to transfer between different locations in the laboratory, thereby improving the overall automation level and flexibility of the equipment.

[0050] In this embodiment, the working principle and workflow of the device are as follows:

[0051] S1: Open the maintenance door 602 of the dehydration component 6, slide the five solvent storage tanks 605 into the housing 601 along the baffle 604, and ensure that the first seal 607 on the rear side of each solvent storage tank 605 is accurately inserted into the second seal 608 on the rear side wall of the housing 601 to achieve a sealed connection. Inject the five storage tanks with solvents of preset concentrations in sequence: 70% ethanol, 80% ethanol, 95% ethanol, anhydrous ethanol, and xylene. Close the maintenance door 602. The spring compression member 625 on the inside of the door is close to the front side of the solvent storage tank 605 to prevent the solvent storage tank 605 from shifting during equipment operation. Preset the parameters for each stage through the controller inside the frame 1: the soaking time of each solvent, the moving path of the transfer component 4, and the opening and closing timing of the cover plate 624. Start the equipment self-test and check the operating status of the acid and alkali resistant centrifugal pump 613, the single-way solenoid valve 609, and the pneumatic suction cup 415 to ensure that each component is fault-free.

[0052] S2: The embedding cassette containing the pathological sample is neatly placed on the tray 5. The controller starts the transfer assembly 4. The first motor 404 drives the first threaded rod 402 to rotate, causing the first threaded seat 405 to move back and forth along the first limiting rod 403, so that the pneumatic suction cup 415 is aligned with the top of the tray 5. The second motor 411 drives the second threaded rod 407 to rotate, causing the second threaded seat 409 and the sliding seat 410 to move left and right along the second limiting rod 408, further fine-tuning the position of the suction cup. The lifting cylinder 413 extends, driving the connecting strip 4 14. The pneumatic suction cup 415 descends, and the air source component supplies air to the suction cup to generate negative pressure, adsorbing the embedding box; the lifting cylinder 413 retracts, and the transfer component 4 moves the embedding box directly above the dehydration cylinder 616; the lifting cylinder 413 extends again, and the embedding box is smoothly placed into the dehydration cylinder 616. The controller starts the servo motor 621, which drives the bidirectional lead screw 620 to rotate, causing the third threaded seats 622 on both sides and the sliding parts 623 to move towards each other along the guide rod 619, so that the cover plate 624 closes, sealing the dehydration cylinder 616 and preventing solvent evaporation.

[0053] S3: The controller opens the single-way solenoid valve 609 corresponding to the 70% ethanol storage tank and simultaneously starts the acid and alkali resistant centrifugal pump 613. The centrifugal pump draws solvent from the combined tank 610 through the inlet pipe 614. The combined tank 610 is connected to the single-way solenoid valve 609 through the connecting hose 611, and then injects 70% ethanol into the dehydration tank 616 through the drain pipe 615 until the embedding box is completely submerged. The centrifugal pump and solenoid valve are closed, and the soaking is carried out for a preset time. After soaking, the drain pipe valve at the bottom of the dehydration tank 616 is opened, and the waste ethanol is discharged into the wastewater tank 617 below. After the wastewater is discharged, the valve is closed, and the above steps are repeated. The single-way solenoid valves 609 corresponding to 80% ethanol, 95% ethanol, anhydrous ethanol, and xylene are opened in sequence to complete the gradient treatment of "low concentration → high concentration → transparency". Before each stage of solvent injection, the controller will briefly turn on the acid and alkali resistant centrifugal pump 613 to run in reverse to empty the solvent of the previous concentration remaining in the combined tank 610 and the drain pipe 615 to avoid cross-contamination.

[0054] S4: After gradient dehydration is completed, cover plate 624 is opened, and transfer component 4 is activated again. Pneumatic suction cup 415 adsorbs the dehydrated embedding box and moves it to the upper part of placement plate 9 on the right side of frame 1. The embedding box is placed stably on placement plate 9. The controller starts electric push rod 10, which pulls placement plate 9 to slide to the right along moving guide rail 7 through connecting piece 11. When placement plate 9 touches positioning contact 12, electric push rod 10 stops. At this time, placement plate 9 is aligned with the paraffin liquid outlet of paraffin embedding machine 8. Paraffin embedding machine 8 automatically injects molten paraffin into embedding box to complete wax impregnation. After wax impregnation is completed, electric push rod 10 drives placement plate 9 to reset. The embedded box after wax impregnation is removed manually or by automated equipment. The controller closes all solenoid valves and motors and transfers waste solvent to a special treatment container. Inspection door 602 can be opened as needed to replenish solvent in solvent storage tank 605 to prepare for the next batch of sample processing.

[0055] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An adjustable automated dehydration and paraffin impregnation machine for pathological samples, characterized in that: Includes a frame (1), a vertical plate (2) is fixedly connected to the rear side of the frame (1), an installation beam (3) is fixedly connected to the upper front side of the vertical plate (2), a transfer component (4) is installed at the bottom of the installation beam (3), and a dehydration component (6) is provided in the middle position inside the frame (1). The dehydration assembly (6) includes a housing (601), which is fixedly connected to the middle position inside the frame (1). At least six baffles (604) are fixedly connected to the bottom surface inside the housing (601). A solvent storage tank (605) is slidably connected between two adjacent baffles (604). The rear side wall of the housing (601) is fixedly connected with the same number of second seals (608) as the solvent storage tanks (605) at the corresponding positions. A first seal (607) is fixedly connected to the rear side of each of the five solvent storage tanks (605). The first seal (607) is inserted into the second seal (608). A suction tube (606) is fixedly connected to the front side of the first seal (607) inside the solvent storage tank (605). A single-way solenoid valve (609) is fixedly connected to the rear side of each of the five second seals (608) through a flange.

2. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 1, characterized in that: A bracket (5) for placing embedding boxes is fixedly connected to the upper left side of the frame (1). Two sets of moving guide rails (7) are fixedly connected to the upper right side of the frame (1). A placement plate (9) is slidably connected above the two sets of moving guide rails (7) via sliding blocks. A paraffin embedding machine (8) is fixedly installed on the upper side of the frame (1) behind the moving guide rails (7). An electric push rod (10) is fixedly connected to the upper side of the frame (1) in front of the moving guide rails (7). A connecting piece (11) is fixedly connected to the output end of the electric push rod (10). The rear side of the connecting piece (11) is fixedly connected to the front side of the placement plate (9). A positioning contact (12) is fixedly connected between the two sets of moving guide rails (7) on the upper right side of the frame (1).

3. An adjustable automated dehydration and paraffin-impregnation machine for pathological samples according to any one of claims 1-2, characterized in that: A partition (603) is fixedly connected to the middle position inside the outer shell (601). A wastewater tank (617) is detachably connected above the partition (603). A dehydration cylinder (616) is fixedly connected to the top wall inside the outer shell (601). A drain pipe with a valve is provided at the bottom of the dehydration cylinder (616). The drain pipe is located directly above the inlet of the wastewater tank (617). An inspection door (602) is rotatably connected to the front side of the outer shell (601) via a hinge. A spring compression piece (625) with the same number as the solvent storage tank (605) is fixedly connected to the lower inner side of the inspection door (602). An observation window for observation is provided on the front side of the inspection door (602).

4. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 3, characterized in that: A merging box (610) is fixedly connected to the middle of the rear side of the outer shell (601). The bottom of the merging box (610) is fixedly connected to the same number of connecting hoses (611) as the solvent storage tank (605). The ends of the five sets of connecting hoses (611) are respectively connected to five single-way solenoid valves (609). A connecting plate (612) is fixedly connected to the upper rear side inside the outer shell (601). An acid and alkali resistant centrifugal pump (613) is fixedly connected to the upper part of the connecting plate (612). An inlet pipe (614) is fixedly connected to the input end of the acid and alkali resistant centrifugal pump (613). The end of the inlet pipe (614) passes through the rear side wall of the outer shell (601) and is fixedly connected to the upper part of the merging box (610). A drain pipe (615) is fixedly connected to the output end of the acid and alkali resistant centrifugal pump (613). The end of the drain pipe (615) is connected to the inside of the dehydration tank (616).

5. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 4, characterized in that: Fixed plates (618) are fixedly connected to the top left and right sides of the outer shell (601). A bidirectional lead screw (620) is rotatably connected between the two fixed plates (618) on the rear side. The two ends of the outer surface of the bidirectional lead screw (620) are threaded with third thread seats (622). A guide rod (619) is fixedly connected to the front side between the two fixed plates (618). Sliding parts (623) are slidably connected to the two ends of the outer surface of the guide rod (619). Cover plates (624) are fixedly connected between the third thread seats (622) and the sliding parts (623) on the left and right sides. A servo motor (621) for driving the bidirectional lead screw (620) to rotate is fixedly installed on the side of the fixed plate (618) on the left side.

6. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 1, characterized in that: The transfer assembly (4) includes two fixed frames (401), which are fixedly connected to the left and right sides of the bottom of the mounting beam (3), respectively. A first threaded rod (402) is rotatably connected inside each of the two fixed frames (401). A first limiting rod (403) is fixedly connected below the first threaded rod (402) inside each of the two fixed frames (401). A first threaded seat (405) is threadedly connected to the outer surface of each of the two first threaded rods (402). The first threaded seat (405) and the first limiting rod (403) are slidably connected through a guide sleeve. A first motor (404) for driving the first threaded rod (402) to rotate is fixedly connected to the front side of each of the two fixed frames (401).

7. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 6, characterized in that: Each of the two first threaded seats (405) is fixedly connected to a connecting seat (406) at its bottom. A second threaded rod (407) is rotatably connected between the two connecting seats (406) at their rear sides. A second threaded seat (409) is threadedly connected to the outer surface of the second threaded rod (407). A second limiting rod (408) is fixedly connected between the two connecting seats (406) at their front sides. A sliding seat (410) is slidably connected to the outer surface of the second limiting rod (408). The rear side of the sliding seat (410) is fixedly connected to the front side of the second threaded seat (409). A second motor (411) for driving the second threaded rod (407) to rotate is fixedly connected to the side of at least one of the connecting seats (406).

8. The adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 7, characterized in that: A fixed seat (412) is fixedly connected to the front side of the sliding seat (410). A lifting cylinder (413) is fixedly connected above the fixed seat (412). The output end of the lifting cylinder (413) passes through the bottom surface of the fixed seat (412) and is fixedly connected to a connecting strip (414). A pneumatic suction cup (415) for adsorbing the embedding box is fixedly connected to the bottom of the connecting strip (414).

9. An adjustable automated dehydration and paraffin impregnation machine for pathological samples according to claim 1, characterized in that: The frame (1) is also equipped with a controller and a gas supply component for gas supply, and the four corners of the bottom of the frame (1) are all fixedly connected with universal casters for moving the device.

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