A multi-mode mechanical testing apparatus for aortic dissection membrane and a testing method thereof

Abstract

The present application relates to aortic dissection membrane mechanics measuring technical field, and disclose a kind of for aortic dissection membrane piece Multi-mode mechanics measuring equipment and its measuring method, including equipment box body, the top of the equipment box body is rotatably connected with top cover.The Multi-mode mechanics measuring equipment for aortic dissection membrane piece and its measuring method, by compacting spring, adjusting nut and floating clamping seat constitute flexible clamping structure, replace traditional rigid fixture, by nut control spring compression amount adaptation different thickness interlayer membrane piece, lifting rotary mechanism is completed after clamping in low-temperature sample carrier, aortic dissection membrane piece is moved to water bath station;Constant-temperature water bath soaking membrane piece lower end, supporting nozzle sprays 37 ℃ physiological saline, simulate in-vivo physiological wet environment, fixed aortic dissection membrane piece initial strain, eliminate the error caused by dry state test and clamping deformation, improve the detection precision of elastic modulus, tensile strength.

Description

Technical Field

[0001] This invention relates to the field of mechanical measurement technology for aortic dissection membranes, specifically to a multi-mode mechanical measurement device and method for aortic dissection membranes. Background Technology

[0002] The mechanical properties of the intimal flap in aortic dissection are key factors that determine the extension of the dissection tear, luminal collapse, and long-term vascular remodeling. In clinical practice and scientific research, it is necessary to accurately measure multiple mechanical indicators such as the elastic modulus, tear strength, fracture toughness, and anisotropy of the flap in order to reveal the pathogenesis and progression of dissection. Therefore, multi-parameter mechanical testing of ex vivo dissection flaps has become an important experimental tool for basic cardiovascular research and personalized diagnosis and treatment.

[0003] Currently, most mechanical testing of aortic dissection diaphragms relies on universal testing machines or atomic force microscopes for ex vivo tissue analysis. However, existing testing equipment still has several shortcomings. First, conventional tests are mostly conducted in a dry, room-temperature environment, which cannot replicate the in vivo environment of 37°C body temperature, tissue surface moisture, and physiological pre-tension. The mechanical properties of the diaphragm change under varying conditions of dryness, humidity, and temperature, resulting in significant discrepancies between the measured elasticity and tear-related data and the actual in vivo mechanical performance, thus limiting the clinical reference value of the test data. Second, existing equipment has a single testing mode; a single device can only perform tensile or tear tests. If multi-mode testing is required, the aortic dissection diaphragm must be repeatedly disassembled and replaced, which not only leads to low testing efficiency but also reduces the consistency of data from the same group due to assembly errors caused by multiple clamping. Furthermore, mice are commonly used experimental animals for constructing aortic dissection disease models, and their arterial blood vessel size is much smaller than that of human blood vessels. This device adopts a modular design, and based on the detection of human aortic dissection diaphragms, it can replace small-sized clamping accessories to adapt to the clamping and mechanical testing of isolated mouse arterial samples, effectively expanding the application scenarios of the device. Summary of the Invention

[0004] This invention provides a multi-mode mechanical measurement device and method for aortic dissection membranes, which solves the problems mentioned in the background art.

[0005] The present invention provides the following technical solution: a multi-mode mechanical measurement device for aortic dissection membranes, comprising a device housing, a top cover rotatably connected to the top of the device housing, a control console mounted on the top of the top cover, a rotating cover rotatably connected to the top of the device housing, an electron microscope mounted on the top of the rotating cover, a testing component located at the bottom of the inner cavity of the device housing, a water bath component located at the bottom of the inner cavity of the device housing, a sample tray fixedly mounted at the bottom of the inner cavity of the device housing, and an ice storage box movably fitted into the inner cavity of the sample tray.

[0006] As a preferred embodiment of the present invention: the test assembly includes a lifting base, a guide cylinder seat fixedly mounted on the inner wall of the lifting base, a guide column fixedly mounted on the inner cavity of the guide cylinder seat, a first electric push rod fixedly mounted on the bottom of the lifting base, a rotating support seat movably sleeved on the outer wall of the guide column, a lifting sliding seat fixedly mounted on the bottom of the rotating support seat, a second electric push rod fixedly mounted on the bottom of the rotating support seat, a drive rack fixedly mounted on the output shaft of the second electric push rod, a guide frame fixedly mounted on the bottom of the rotating support seat, a slewing support bearing seat mounted on the top of the rotating support seat, a slewing bearing platform rotatably connected to the inner cavity of the slewing support bearing seat, and a driven gear fixedly mounted on the bottom of the slewing bearing platform.

[0007] As a preferred embodiment of the present invention: a chuck mounting rail is fixedly mounted on the top of the rotary bearing platform, a tension drive motor is fixedly mounted in the inner cavity of the chuck mounting rail, a transmission lead screw is fixedly mounted on the output shaft of the tension drive motor, a left sliding clamp is movably sleeved in the inner cavity of the chuck mounting rail, and a right sliding clamp is movably sleeved in the inner cavity of the chuck mounting rail.

[0008] As a preferred embodiment of the present invention: a tension sensor is fixedly mounted on the top of the rotary bearing platform; a deflection support is fixedly mounted on the outer wall of the left sliding clamp; a deflection limiting groove is provided on the top of the deflection support; a deflection base is rotatably connected to the top of the left sliding clamp; a driven meshing gear is fixedly mounted on the bottom of the deflection base; a clamping screw is fixedly mounted on the top of the deflection base; a floating clamping seat is movably sleeved on the outer wall of the clamping screw; a clamping spring is movably sleeved on the outer wall of the clamping screw; an adjusting nut is threadedly connected to the outer wall of the clamping screw; a motor fixing chamber is fixedly mounted on the bottom of the left sliding clamp; a tearing drive motor is fixedly mounted on the bottom of the inner cavity of the motor fixing chamber; and a drive gear is fixedly mounted on the power output shaft of the tearing drive motor.

[0009] As a preferred embodiment of the present invention: the output shaft of the first electric push rod passes through the inner cavity of the lifting base and is connected to the bottom of the lifting sliding seat; the guide frame is embedded and movably sleeved on the inner wall of the drive rack; and the end of the drive rack away from the guide frame meshes with the driven gear.

[0010] As a preferred embodiment of the present invention: the rotary bearing platform penetrates downward through the inner cavity of the rotary support bearing seat, the outer wall of the transmission screw is threadedly connected to the inner walls of the left sliding clamp and the right sliding clamp respectively, the outer wall shape of the left sliding clamp and the right sliding clamp near the end of the chuck mounting rail matches the inner wall shape of the chuck mounting rail, and the two ends of the tension sensor are connected to the outer walls of the left sliding clamp and the right sliding clamp respectively.

[0011] As a preferred technical solution of the present invention: the end of the deflection base away from the driven meshing gear is movably sleeved in the inner cavity of the deflection limiting groove, the deflection base passes downward through the inner wall of the left sliding clamp and is connected to the driven meshing gear, the two ends of the tension drive motor are respectively connected to the top of the floating clamp and the bottom of the adjusting nut, and the drive gear and the driven meshing gear mesh with each other.

[0012] As a preferred embodiment of the present invention: the water bath assembly includes a constant temperature water bath cylinder, a nozzle mounting bracket is fixedly mounted on the outer wall of the constant temperature water bath cylinder, an atomizing spray pipe is fixedly mounted on the outer wall of the nozzle mounting bracket, a delivery pipe is fixedly mounted on the top of the atomizing spray pipe, a liquid storage tank is fixedly mounted on the bottom of the inner cavity of the equipment box, a water pump is built into the inner cavity of the liquid storage tank, and the water outlet pipe of the water pump is connected to the delivery pipe.

[0013] As a preferred embodiment of the present invention: the end of the delivery pipe away from the atomizing nozzle is connected to the top of the liquid storage tank; there are two nozzle mounting brackets, and the two nozzle mounting brackets are respectively installed on both sides of the outer wall of the constant temperature water bath; and a release hole is provided on the outer wall of the atomizing nozzle.

[0014] A measurement method for a multi-mode biomechanical measurement device for aortic dissection patches includes the following steps: S1. Fill the inner cavity of the sample tray with ice, and place multiple aortic dissection membrane pieces in sequence on the upper end of the sample tray for low-temperature preservation; add sterile saline to the storage tank, start the built-in water pump in the storage tank, and send the sterile saline through the delivery pipe and atomizing nozzle into the inner cavity of the constant temperature water bath. calibrate the liquid level in the constant temperature water bath to ensure that the spray medium of the atomizing nozzle is consistent with the temperature and composition of the water bath liquid. S2. Initially, the left and right sliding clamps are positioned above the sample tray. The membrane is placed between the deflection base and the floating clamp on the top surface of the sample tray. The adjusting nut is rotated to compress the clamping spring, which elastically clamps the floating clamp and flexibly holds the membrane in cooperation with the deflection base. The first electric push rod is driven to lift the lifting sliding seat, which moves the rotating support along the guide column, raising the entire rotary bearing platform. Then, the second electric push rod drives the drive rack to slide along the guide frame, which meshes with the driven gear, rotating the entire rotary bearing platform 180°. This allows the left and right sliding clamps, along with the membrane they hold, to be transferred to the top of the constant temperature water bath. Subsequently, the first electric push rod is controlled to lower the lifting sliding seat, the rotating support, and the rotary bearing platform, immersing the lower part of the membrane in the physiological saline inside the constant temperature water bath. S3. The constant temperature water bath is maintained at 37℃. The atomizing nozzle continuously atomizes and sprays physiological saline at the same temperature, fully covering the upper surface of the membrane. The tension drive motor and transmission screw drive the left and right sliding clamps to separate slightly, applying physiological pretension to the membrane and locking the initial strain of the aortic dissection membrane.

[0015] S4. Observe the surface of the scanning membrane using an electron microscope and mark the areas to be tested for calcification, tearing, and hematoma; then perform a tensile test: the tensile drive motor drives the double-sided transmission screws to rotate synchronously, the left and right sliding clamps move away from each other at a uniform speed in opposite directions, the tensile sensor collects tensile data in real time, and calculates the elastic modulus and tensile strength; after the tensile test is completed, remove the damaged aortic dissection membrane. The tear test was then conducted: the rotary support platform was rotated back above the sample tray, a new aortic dissection membrane was installed, and the S2-S3 clamping and environmental control steps were repeated; the tear drive motor drove the drive gear to mesh, which in turn caused the driven meshing gear and the deflection base to deflect, and the clamps on both sides rotated outward to pull the aortic dissection membrane, thus tearing the aortic dissection membrane on both sides. S5. After the single sample test is completed, all components are reset. The rotary bearing platform is rotated back to above the sample tray. The aortic dissection diaphragm to be tested on the sample tray is replaced. S2 to S4 are executed in a cycle to complete the mechanical testing of all diaphragms in batches.

[0016] The present invention has the following beneficial effects: 1. This multi-mode mechanical testing device and method for aortic dissection membranes utilizes a flexible clamping mechanism comprised of a compression spring, an adjusting nut, and a floating clamping seat to replace traditional rigid clamps. The clamping force is precisely controlled by adjusting the spring compression with the nut, adapting to membranes of varying thicknesses and preventing clamping, crushing, or slippage. The lifting and rotating transport mechanism allows clamping at the sample tray with an ice storage box before transferring the aortic dissection membrane to the water bath station. The lower end of the membrane is immersed in a constant-temperature water bath, while sprays of 37°C physiological saline from both sides ensure the aortic dissection membrane remains in a physiologically warm and humid environment throughout the test. Before testing, a slight displacement of the driving clamping seat applies physiological pre-tension, fixing the initial strain of the aortic dissection membrane and overcoming data deviations caused by traditional room-temperature dry testing and clamping deformation. This effectively improves the accuracy of mechanical parameters such as elastic modulus and tensile strength.

[0017] 2. This multi-mode mechanical testing device and method for aortic dissection membranes features an integrated tensile and tear testing structure. The tensile test is achieved by a tension drive motor driving a bidirectional transmission screw, which synchronously moves the left and right clamps in opposite directions. The tear test is performed by a tear drive motor in conjunction with gear meshing, which drives the deflection of a single clamp to achieve tear detection. Both mechanical properties can be measured without changing the clamps. The device is equipped with a sample tray with a built-in ice storage box, continuously keeping the membrane under test at low temperatures to prevent denaturation and failure after sample removal. An electron microscope mounted on the top allows for real-time observation and marking of characteristic areas such as tears, calcification, and hematomas, facilitating targeted testing. A single clamping station can cycle between the sample loading area and the water bath testing area, enabling automated continuous screening of multiple samples. This significantly reduces the time spent on frequent sample changes and repeated clamping, minimizes human error during clamping, and meets the needs of large-scale clinical research trials. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a schematic diagram of the equipment housing structure of the present invention; Figure 3 This is a schematic diagram of the rotating cover structure of the present invention; Figure 4 This is a schematic diagram of the sample carrier disk structure of the present invention; Figure 5 This is a schematic diagram of the ice storage box structure of the present invention; Figure 6 This is a schematic diagram of the water bath structure of the present invention; Figure 7 This is a schematic diagram of the lifting base structure of the present invention; Figure 8 This is a schematic diagram of the rotating support structure of the present invention; Figure 9 This is a schematic diagram of the drive rack structure of the present invention; Figure 10 This is a schematic diagram of the clamp mounting rail structure of the present invention; Figure 11 This is a schematic diagram of the deflection support structure of the present invention; Figure 12 This is a schematic diagram of the deflection base structure of the present invention.

[0019] In the diagram: 1. Equipment housing; 2. Top cover; 3. Control console; 4. Rotating cover; 5. Electron microscope; 6. Testing components; 7. Water bath components; 8. Sample tray; 9. Ice storage box; 601. Lifting base; 602. Guide cylinder seat; 603. Guide column; 604. First electric push rod; 605. Lifting sliding seat; 606. Rotary support seat; 607. Second electric push rod; 608. Drive rack; 609. Guide frame; 6010. Rotary support bearing seat; 6011. Rotary bearing platform; 6012. Driven gear; 6013. Chuck mounting rail; 6014. Tension drive motor; 6015. Transmission screw 6016, Left sliding clamp; 6017, Right sliding clamp; 6018, Deflection support; 6019, Deflection limiting groove; 6020, Deflection base; 6021, Driven meshing gear; 6022, Clamping screw; 6023, Floating clamp; 6024, Clamping spring; 6025, Adjusting nut; 6026, Tear drive motor; 6027, Drive gear; 6028, Motor fixing chamber; 6029, Tension sensor; 701. Constant temperature water bath; 702. Nozzle mounting bracket; 703. Atomizing nozzle; 704. Delivery pipe; 705. Liquid storage tank. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Please see Figures 1-12 A multi-mode mechanical measurement device for aortic dissection membranes includes a device housing 1, a top cover 2 rotatably connected to the top of the device housing 1, a control console 3 mounted on the top of the top cover 2, a rotating cover 4 rotatably connected to the top of the device housing 1, an electron microscope 5 mounted on the top of the rotating cover 4, a test assembly 6 at the bottom of the inner cavity of the device housing 1, a water bath assembly 7 at the bottom of the inner cavity of the device housing 1, a sample tray 8 fixedly mounted at the bottom of the inner cavity of the device housing 1, and an ice storage box 9 movably fitted into the inner cavity of the sample tray 8.

[0022] In the above structure, the sample tray 8 and ice storage box 9 are set in the inner cavity of the equipment box 1. The aortic dissection membrane is placed on top of the sample tray 8, and ice is placed in the inner cavity of the ice storage box 9 to preserve the aortic dissection membrane on top of the sample tray 8, so as to facilitate the subsequent tensile test of the aortic dissection membrane.

[0023] In a preferred embodiment: the test assembly 6 includes a lifting base 601, a guide cylinder seat 602 fixedly mounted on the inner wall of the lifting base 601, a guide column 603 fixedly mounted on the inner cavity of the guide cylinder seat 602, a first electric push rod 604 fixedly mounted on the bottom of the lifting base 601, a rotating support seat 606 movably sleeved on the outer wall of the guide column 603, a lifting sliding seat 605 fixedly mounted on the bottom of the rotating support seat 606, a second electric push rod 607 fixedly mounted on the bottom of the rotating support seat 606, a drive rack 608 fixedly mounted on the output shaft of the second electric push rod 607, a guide frame 609 fixedly mounted on the bottom of the rotating support seat 606, a slewing support bearing seat 6010 mounted on the top of the rotating support seat 606, a slewing bearing platform 6011 rotatably connected to the inner cavity of the slewing support bearing seat 6010, and a driven gear 6012 fixedly mounted on the bottom of the slewing bearing platform 6011.

[0024] In the above structure, a guide column 603 is installed in the inner cavity of the guide cylinder seat 602 and extends through the inner cavity of the rotating support seat 606. This allows the first electric push rod 604 to lift the lifting sliding seat 605 upwards, thereby driving the lifting sliding seat 605 and the rotating support seat 606 to rise along the outer wall of the guide column 603. This adjusts the height of the rotary bearing platform 6011. The second electric push rod 607 drives the drive rack 608, causing the drive rack to... 608 can slide along the outer wall of the guide frame 609, and under the sliding action of the drive rack 608, it drives the driven gear 6012, so that the rotary bearing platform 6011 can rotate under the support of the rotary support bearing seat 6010, so that the rotary bearing platform 6011 can realize lifting and rotating actions, so that the transmission screw 6015 and the right sliding clamp 6017 can be flexibly adjusted in position within the inner cavity of the equipment housing 1, so as to perform the stretching and tearing test on the aortic dissection membrane.

[0025] In a preferred embodiment: a chuck mounting rail 6013 is fixedly mounted on the top of the rotary bearing platform 6011, a tension drive motor 6014 is fixedly mounted in the inner cavity of the chuck mounting rail 6013, a transmission lead screw 6015 is fixedly mounted on the output shaft of the tension drive motor 6014, a left sliding clamp 6016 is movably sleeved in the inner cavity of the chuck mounting rail 6013, and a right sliding clamp 6017 is movably sleeved in the inner cavity of the chuck mounting rail 6013.

[0026] In the above structure, the chuck mounting rail 6013 is provided on the top of the rotary bearing platform 6011, and the left sliding chuck 6016 and right sliding chuck 6017 are movably sleeved in the inner cavity of the chuck mounting rail 6013. The transmission screw 6015 is driven by the tension drive motor 6014, thereby allowing the left sliding chuck 6016 and right sliding chuck 6017 to move along the outer wall of the transmission screw 6015, changing the distance between them. As the left sliding chuck 6016 and right sliding chuck 6017 move away from each other, the distance between them is adjusted. A tensile test is performed on the aortic dissection diaphragm on the top of the left sliding clamp 6016 and the right sliding clamp 6017. As the left sliding clamp 6016 and the right sliding clamp 6017 move away from each other, the tension between the left sliding clamp 6016 and the right sliding clamp 6017 is detected by the deflection support 6018. The tension drive motor 6014 is equipped with transmission screws 6015 at both ends, so that when the tension drive motor 6014 drives the transmission screws 6015, the left sliding clamp 6016 and the right sliding clamp 6017 can move synchronously and stretch the diaphragm evenly.

[0027] In a preferred embodiment: a tension sensor 6029 is fixedly mounted on the top of the rotary support platform 6011; a deflection support 6018 is fixedly mounted on the outer wall of the left sliding clamp 6016; a deflection limiting groove 6019 is provided on the top of the deflection support 6018; a deflection base 6020 is rotatably connected to the top of the left sliding clamp 6016; a driven meshing gear 6021 is fixedly mounted on the bottom of the deflection base 6020; and a clamping screw 60 is fixedly mounted on the top of the deflection base 6020. 22. A floating clamping seat 6023 is movably sleeved on the outer wall of the clamping screw 6022. A clamping spring 6024 is movably sleeved on the outer wall of the clamping screw 6022. An adjusting nut 6025 is threadedly connected to the outer wall of the clamping screw 6022. A motor fixing chamber 6028 is fixedly assembled at the bottom of the left sliding clamping seat 6016. A tearing drive motor 6026 is fixedly assembled at the bottom of the inner cavity of the motor fixing chamber 6028. A drive gear 6027 is fixedly assembled on the power output shaft of the tearing drive motor 6026.

[0028] In the above structure, the clamping screw 6022 provided on the top of the deflection base 6020, and the floating clamping seat 6023 movably sleeved on the outer wall of the clamping screw 6022, under the drive of the compressed clamping spring 6024, can make the floating clamping seat 6023 tend to move downward, thereby bringing the floating clamping seat 6023 and the deflection base 6020 closer together. Then, the aortic dissection patch can be applied through the floating clamping seat 6023 and the deflection base 6020. The clamping force of the floating clamping seat 6023 can be adjusted as needed. By adjusting the position of the adjusting nut 6025 on the floating clamping seat 6023, the compression degree of the compression spring 6024 can be adjusted, thereby changing the downward force of the floating clamping seat 6023 on the top of the deflection base 6020. This ensures that the aortic dissection diaphragm is fixed without damaging it, so that the aortic dissection diaphragm can be successfully subjected to tensile tests in the future.

[0029] In a preferred embodiment: the output shaft of the first electric push rod 604 passes through the inner cavity of the lifting base 601 and is connected to the bottom of the lifting sliding seat 605; the guide frame 609 is embedded and movably sleeved on the inner wall of the drive rack 608; and the end of the drive rack 608 away from the guide frame 609 meshes with the driven gear 6012.

[0030] In the above structure, a first electric push rod 604 is installed at the bottom of the lifting base 601. The first electric push rod 604 lifts the lifting sliding seat 605 upward, which pushes the rotating support seat 606 to rise along the outer wall of the guide column 603. This raises the height of the rotating support seat 606 and the rotary bearing platform 6011 as a whole, thereby indirectly adjusting the working height of the right sliding clamp 6017 and the left sliding clamp 6016. The second electric push rod 607 drives the drive rack 608, which slides along the outer wall of the guide frame 609. As the drive rack 608 moves, it drives the driven gear 6012, which in turn drives the driven gear 6012 and the rotary bearing platform 6011 to rotate under the support of the rotary support bearing seat 6010. This adjusts the working position of the left sliding clamp 6016 and the right sliding clamp 6017.

[0031] In a preferred embodiment: the rotary bearing platform 6011 extends downward through the inner cavity of the rotary support bearing seat 6010, the outer wall of the transmission screw 6015 is threadedly connected to the inner walls of the left sliding clamp 6016 and the right sliding clamp 6017 respectively, the outer wall shape of the left sliding clamp 6016 and the right sliding clamp 6017 near the end of the chuck mounting rail 6013 matches the inner wall shape of the chuck mounting rail 6013, and the two ends of the tension sensor 6029 are connected to the outer walls of the left sliding clamp 6016 and the right sliding clamp 6017 respectively.

[0032] In the above structure, a tension drive motor 6014 installed in the inner cavity of the chuck mounting rail 6013 drives the transmission screws 6015 on both sides, allowing the transmission screws 6015 to rotate within the inner cavity of the chuck mounting rail 6013. This, in turn, drives the left sliding chuck 6016 and the right sliding chuck 6017, enabling them to slide along the chuck mounting rail 6013 respectively. The distance between the movable clamps 6017 is adjusted to perform a tensile test on the aortic dissection membrane fixed between the left sliding clamp 6016 and the right sliding clamp 6017. The left sliding clamp 6016 and the right sliding clamp 6017 can move outward synchronously. During the separation of the left sliding clamp 6016 and the right sliding clamp 6017, the tension sensor 6029 can be pulled, thereby recording the tension between the left sliding clamp 6016 and the right sliding clamp 6017 through the tension sensor 6029.

[0033] In a preferred embodiment: the end of the deflection base 6020 away from the driven meshing gear 6021 is movably sleeved in the inner cavity of the deflection limiting groove 6019, the deflection base 6020 extends downward through the inner wall of the left sliding clamp 6016 and is connected to the driven meshing gear 6021, the two ends of the tension drive motor 6014 are respectively connected to the top of the floating clamp 6023 and the bottom of the adjusting nut 6025, and the drive gear 6027 meshes with the driven meshing gear 6021.

[0034] In the above structure, the deflection support 6018 provided on the outer wall of the left sliding clamp 6016 and the deflection limiting groove 6019 opened in the inner cavity of the deflection support 6018 are used to drive the drive gear 6027 by the tearing drive motor 6026, thereby driving the driven meshing gear 6021 to rotate. This allows the deflection base 6020 to rotate on the top of the left sliding clamp 6016 and along the inner wall of the deflection limiting groove 6019. With the deflection of the deflection base 6020 on the top of the left sliding clamp 6016 and the right sliding clamp 6017, the diaphragm held by the floating clamp 6023 and the deflection base 6020 can be torn to both sides, so that the diaphragm can be unfolded and torn outward to perform the tearing operation of the diaphragm.

[0035] In a preferred embodiment: the water bath assembly 7 includes a constant temperature water bath cylinder 701, a nozzle mounting bracket 702 is fixedly mounted on the outer wall of the constant temperature water bath cylinder 701, an atomizing nozzle 703 is fixedly mounted on the outer wall of the nozzle mounting bracket 702, a delivery pipe 704 is fixedly mounted on the top of the atomizing nozzle 703, a liquid storage tank 705 is fixedly mounted on the bottom of the inner cavity of the equipment housing 1, a water pump is built into the inner cavity of the liquid storage tank 705, and the water outlet pipe of the water pump is connected to the delivery pipe 704.

[0036] In the above structure, the constant temperature water bath 701 and the atomizing nozzle 703 installed on the outer wall of the nozzle mounting bracket 702, through the water pump built into the inner cavity of the water storage tank 705, can release sterile physiological saline in the inner cavity of the water storage tank 705 into the delivery pipe 704 by starting the water pump, and release physiological saline into the space at the top of the constant temperature water bath 701 through the atomizing nozzle 703. The sterile physiological saline is continuously atomized and sprayed onto the upper surface of the aortic dissection membrane through the atomizing nozzle 703, so as to achieve uniform wetting of the entire aortic dissection membrane. Moreover, the composition of the spray liquid is consistent with the water bath medium in the inner cavity of the constant temperature water bath 701, which can avoid local dehydration and shrinkage of the sample or osmotic pressure imbalance, and ensure that the entire aortic dissection membrane is always in a physiologically moist condition close to the body.

[0037] In a preferred embodiment: the end of the delivery pipe 704 away from the atomizing nozzle 703 is connected to the top of the liquid storage tank 705, there are two nozzle mounting brackets 702, and the two nozzle mounting brackets 702 are respectively installed on both sides of the outer wall of the constant temperature water bath cylinder 701, and the outer wall of the atomizing nozzle 703 is provided with a release hole.

[0038] In the above structure, a constant temperature water bath 701 is set up, and the inner cavity of the constant temperature water bath 701 is filled with physiological saline. When the aortic dissection diaphragm is subjected to stretching and tearing tests, the height of the left sliding clamp 6016 and the right sliding clamp 6017 is reduced, so that the aortic dissection diaphragm on the top of the left sliding clamp 6016 and the right sliding clamp 6017 is immersed downward into the inner cavity of the constant temperature water bath 701 to simulate the physiological environment in the body. Then, the aortic dissection diaphragm is subjected to stretching and tearing tests. During the stretching and tearing tests, the aortic dissection diaphragm can be observed in real time through an electron microscope 5.

[0039] A measurement method for a multi-mode biomechanical measurement device for aortic dissection patches includes the following steps: S1. Ice blocks are placed inside the sample tray 8, and multiple aortic dissection membranes are placed sequentially on the upper end of the sample tray 8 for low-temperature preservation. Sterile physiological saline is added to the storage tank 705, and the built-in water pump of the storage tank 705 is started to send the sterile physiological saline into the inner cavity of the constant temperature water bath 701 through the delivery pipe 704 and the atomizing nozzle 703. The liquid level in the constant temperature water bath 701 is calibrated to ensure that the spray medium of the atomizing nozzle 703 is consistent with the temperature and composition of the water bath liquid. S2. Initially, the left sliding clamp 6016 and right sliding clamp 6017 are positioned above the sample tray 8. The diaphragm is placed between the deflection base 6020 and the floating clamp 6023 on the top surface of the sample tray 8. Rotating the adjusting nut 6025 compresses the clamping spring 6024, elastically clamping the floating clamp 6023, which, in conjunction with the deflection base 6023, flexibly clamps the diaphragm. Simultaneously, the first electric push rod 604 is driven to raise the lifting sliding seat 605, causing the rotating support seat 606 to move upwards along the guide column 603, thus raising the entire rotary bearing platform 6011. Then, the second electric push rod 607 drives the drive rack 608 to slide along the guide frame 609, and the drive rack 608 meshes with the driven gear 6012, causing the rotary bearing platform 6011 to rotate 180°, so that the left sliding clamp 6016 and the right sliding clamp 6017, together with the clamped membrane, are transferred to the top of the constant temperature water bath 701; then, the first electric push rod 604 controls the lifting sliding seat 605, the rotating support seat 606 and the rotary bearing platform 6011 to descend, so that the lower part of the membrane is immersed in the physiological saline inside the constant temperature water bath 701; S3, the constant temperature water bath 701 maintains a constant temperature of 37℃, and the atomizing nozzle 703 continuously atomizes and sprays physiological saline at the same temperature, fully covering the upper surface of the membrane; relying on the tension drive motor 6014 and the transmission screw 6015 to slightly drive the left sliding clamp 6016 and the right sliding clamp 6017 to slightly separate, apply physiological pretension to the membrane, and lock the initial strain of the aortic dissection membrane.

[0040] S4. The surface of the scanning membrane is observed using an electron microscope 5, and the areas to be tested, including calcification, rupture, and hematoma, are marked. Then, a tensile test is performed: the tensile drive motor 6014 drives the double-sided transmission screws 6015 to rotate synchronously, and the left sliding clamp 6016 and the right sliding clamp 6017 move away from each other at a uniform speed in opposite directions. The tensile sensor 6029 collects tensile data in real time and calculates the elastic modulus and tensile strength. After the tensile test is completed, the damaged aortic dissection membrane is removed. The tear test was then conducted: the rotary support platform 6011 rotated back above the sample tray 8, a new aortic dissection membrane was installed, and the clamping and environmental control steps S2-S3 were repeated; the tear drive motor 6026 drove the drive gear 6027 to mesh, which in turn drove the driven meshing gear 6021 and the deflection base 6020 to deflect, and the clamps on both sides rotated outward to pull the aortic dissection membrane, thus tearing the aortic dissection membrane on both sides; S5. After the single sample test is completed, all components are reset. The rotary bearing platform 6011 is rotated and reset to above the sample carrier plate 8. The aortic dissection membrane to be tested on the sample carrier plate 8 is replaced. S2 to S4 are executed in a cycle to complete the mechanical testing of all membranes in batches.

[0041] Example 1: Multimodal biomechanical measurement experiment based on isolated mouse aorta; This embodiment utilizes the aforementioned equipment to conduct a mechanical measurement experiment on isolated mouse aortas. The original standard clamping assembly was removed and replaced with a mouse-specific miniature clamping assembly. The effective clamping width of the miniature clamps was 0.4-1.3 cm, matching the outer diameter of mouse thoracic and abdominal aortas (0.6-1.2 cm). A micro-force compression spring was also installed. A miniature water bath measuring 15 cm long × 5 cm wide × 8 cm deep was embedded within a constant-temperature water bath, equipped with a low-flow directional atomizing nozzle. A 2 cm × 10 cm miniature storage compartment was used to store the vascular samples. A 2-3 cm segment of mouse artery was cut and placed in the miniature storage compartment for cryopreservation. Following the original equipment procedure, sample clamping, 180° rotation transport, 37°C constant-temperature water bath, and atomization spray treatment were performed. Physiological pre-tension was applied through slight displacement of the clamp. The electron microscope was set to high-magnification mode to mark the vascular lesion area. Simultaneously, the equipment operating speed was reduced, and the micro-force range of the tension sensor was switched to perform tensile and tear tests on the mouse arteries. The device automatically resets after each experiment, allowing for continuous testing of multiple batches of samples. By changing the small clamps and accessories, this equipment can be adapted for testing mouse microvascular samples, enabling universal testing of human aortic dissection membranes and animal vascular samples.

[0042] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended technical solutions and their equivalents.

Claims

1. A multi-mode biomechanical measurement device for aortic dissection membranes, comprising a device housing (1), characterized in that: The top of the equipment housing (1) is rotatably connected to a top cover (2), and a control console (3) is installed on the top of the top cover (2). The top of the equipment housing (1) is rotatably connected to a rotating cover (4), and an electron microscope (5) is installed on the top of the rotating cover (4). A test assembly (6) is provided at the bottom of the inner cavity of the equipment housing (1). A water bath assembly (7) is provided at the bottom of the inner cavity of the equipment housing (1). A sample tray (8) is fixedly assembled at the bottom of the inner cavity of the equipment housing (1). An ice storage box (9) is movably sleeved in the inner cavity of the sample tray (8).

2. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 1, characterized in that: The test assembly (6) includes a lifting base (601), a guide cylinder seat (602) fixedly mounted on the inner wall of the lifting base (601), a guide column (603) fixedly mounted on the inner cavity of the guide cylinder seat (602), a first electric push rod (604) fixedly mounted on the bottom of the lifting base (601), a rotating support seat (606) movably sleeved on the outer wall of the guide column (603), a lifting sliding seat (605) fixedly mounted on the bottom of the rotating support seat (603), and the rotating support seat (604)... 6) The bottom is fixedly equipped with a second electric push rod (607), the output shaft of the second electric push rod (607) is fixedly equipped with a drive rack (608), the bottom of the rotary support base (606) is fixedly equipped with a guide frame (609), the top of the rotary support base (606) is equipped with a slewing support bearing seat (6010), the inner cavity of the slewing support bearing seat (6010) is rotatably connected to a slewing bearing platform (6011), and the bottom of the slewing bearing platform (6011) is fixedly equipped with a driven gear (6012).

3. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 2, characterized in that: The top of the rotary bearing platform (6011) is fixedly equipped with a chuck mounting rail (6013), the inner cavity of the chuck mounting rail (6013) is fixedly equipped with a tension drive motor (6014), the output shaft of the tension drive motor (6014) is fixedly equipped with a transmission screw (6015), the inner cavity of the chuck mounting rail (6013) is movably sleeved with a left sliding clamp (6016), and the inner cavity of the chuck mounting rail (6013) is movably sleeved with a right sliding clamp (6017).

4. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 3, characterized in that: A tension sensor (6029) is fixedly mounted on the top of the rotary bearing platform (6011). A deflection support (6018) is fixedly mounted on the outer wall of the left sliding clamp (6016). A deflection limiting groove (6019) is opened on the top of the deflection support (6018). A deflection base (6020) is rotatably connected to the top of the left sliding clamp (6016). A driven meshing gear (6021) is fixedly mounted on the bottom of the deflection base (6020). A clamping screw (6022) is fixedly mounted on the top of the deflection base (6020). A floating clamping seat (6023) is movably sleeved on the outer wall of the tightening screw (6022), a clamping spring (6024) is movably sleeved on the outer wall of the tightening screw (6022), an adjusting nut (6025) is threadedly connected to the outer wall of the clamping screw (6022), a motor fixing chamber (6028) is fixedly assembled at the bottom of the left sliding clamp (6016), a tearing drive motor (6026) is fixedly assembled at the bottom of the inner cavity of the motor fixing chamber (6028), and a drive gear (6027) is fixedly assembled on the power output shaft of the tearing drive motor (6026).

5. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 4, characterized in that: The output shaft of the first electric push rod (604) passes through the inner cavity of the lifting base (601) and is connected to the bottom of the lifting sliding seat (605). The guide frame (609) is embedded and movably sleeved on the inner wall of the drive rack (608). The end of the drive rack (608) away from the guide frame (609) meshes with the driven gear (6012).

6. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 5, characterized in that: The rotary bearing platform (6011) extends downward through the inner cavity of the rotary support bearing seat (6010). The outer wall of the transmission screw (6015) is threadedly connected to the inner walls of the left sliding clamp (6016) and the right sliding clamp (6017). The outer wall shape of the left sliding clamp (6016) and the right sliding clamp (6017) near the chuck mounting rail (6013) matches the inner wall shape of the chuck mounting rail (6013). The two ends of the tension sensor (6029) are connected to the outer walls of the left sliding clamp (6016) and the right sliding clamp (6017) respectively.

7. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 6, characterized in that: The deflection base (6020) is movably sleeved in the inner cavity of the deflection limiting groove (6019) at one end away from the driven meshing gear (6021). The deflection base (6020) extends downward through the inner wall of the left sliding clamp (6016) and is connected to the driven meshing gear (6021). The two ends of the tension drive motor (6014) are respectively connected to the top of the floating clamp (6023) and the bottom of the adjusting nut (6025). The drive gear (6027) meshes with the driven meshing gear (6021).

8. The multi-mode biomechanical measurement device for aortic dissection membranes according to claim 3, characterized in that: The water bath assembly (7) includes a constant temperature water bath cylinder (701), a nozzle mounting bracket (702) is fixedly mounted on the outer wall of the constant temperature water bath cylinder (701), an atomizing nozzle (703) is fixedly mounted on the outer wall of the nozzle mounting bracket (702), a delivery pipe (704) is fixedly mounted on the top of the atomizing nozzle (703), a liquid storage tank (705) is fixedly mounted on the bottom of the inner cavity of the equipment box (1), a water pump is built into the inner cavity of the liquid storage tank (705), and the water outlet pipe of the water pump is connected to the delivery pipe (704).

9. A multi-mode biomechanical measurement device for aortic dissection membranes according to claim 8, characterized in that: The end of the delivery pipe (704) away from the atomizing nozzle (703) is connected to the top of the liquid storage tank (705). There are two nozzle mounting brackets (702), and the two nozzle mounting brackets (702) are respectively installed on both sides of the outer wall of the constant temperature water bath (701). The outer wall of the atomizing nozzle (703) is provided with a release hole.

10. A method for measuring the multi-mode biomechanics of aortic dissection membranes according to any one of claims 1-9. Includes the following steps: Characterized by: S1. Fill the inner cavity of the sample tray (8) with ice, and place multiple aortic dissection membranes in sequence on the upper end of the sample tray (8) for low-temperature preservation; add sterile saline to the storage tank (705), start the built-in water pump of the storage tank (705), and send the sterile saline through the delivery pipe (704) and the atomizing nozzle (703) into the inner cavity of the constant temperature water bath (701). calibrate the liquid level in the constant temperature water bath (701) so that the spray medium of the atomizing nozzle (703) is consistent with the temperature and composition of the water bath liquid; S2. Initially, the left sliding clamp (6016) and right sliding clamp (6017) are located above the sample tray (8). The diaphragm is placed between the deflection base (6020) and the floating clamp (6023) on the top surface of the sample tray (8). The adjusting nut (6025) is rotated to compress the clamping spring (6024), which elastically clamps the floating clamp (6023) and flexibly clamps the diaphragm in cooperation with the deflection base (6020). The first electric push rod (604) is driven to lift the lifting sliding seat (605), which drives the rotating support seat (606) to move upward along the guide column (603), so that the rotary bearing platform (6011) is raised as a whole. The second electric push rod (607) drives the drive rack (608) to slide along the guide frame (609), and the drive rack (608) meshes with the driven gear (6012), causing the rotary bearing platform (6011) to rotate 180°, so that the left sliding clamp (6016) and the right sliding clamp (6017) along with the clamped membrane are transferred to the top of the constant temperature water bath (701); then the first electric push rod (604) controls the lifting sliding seat (605), the rotating support seat (606) and the rotary bearing platform (6011) to descend, so that the lower part of the membrane is immersed in the physiological saline inside the constant temperature water bath (701); S3. The constant temperature water bath (701) maintains a constant temperature of 37°C, and the atomizing nozzle (703) continuously atomizes and sprays physiological saline at the same temperature, fully covering the upper surface of the membrane. The tension drive motor (6014) and the transmission screw (6015) drive the left sliding clamp (6016) and the right sliding clamp (6017) to slightly separate, apply physiological pretension to the membrane, and lock the initial strain of the aortic dissection membrane. S4. The surface of the scanning membrane is observed using an electron microscope (5), and the areas to be tested for calcification, rupture, and hematoma are marked. Then, a tensile test is performed: the tensile drive motor (6014) drives the double-sided transmission screws (6015) to rotate synchronously, and the left sliding clamp (6016) and the right sliding clamp (6017) move away from each other at a constant speed in opposite directions. The tensile sensor (6029) collects tensile data in real time and calculates the elastic modulus and tensile strength. After the tensile test is completed, the damaged aortic dissection membrane is removed. The tear test was then performed: the rotating support platform (6011) was rotated back to the sample tray (8), a new interlayer membrane was installed, and the clamping and environmental control steps of S2-S3 were repeated; the tear drive motor (6026) drove the drive gear (6027) to mesh and drive the driven meshing gear (6021) and the deflection base (6020) to deflect, and the clamps on both sides rotated outward to pull the aortic interlayer membrane, and the two sides of the aortic interlayer membrane were pulled and torn. S5. After the single sample test is completed, each component is reset. The rotary bearing platform (6011) is rotated and reset to above the sample carrier plate (8). The aortic dissection membrane to be tested on the sample carrier plate (8) is replaced. S2 to S4 are executed in a cycle to complete the mechanical test of all membranes in batches.

Images