A pesticide composition and use thereof

Abstract

The application belongs to the technical field of pesticide fungicidal technology, and discloses a kind of pesticide composition and its application, the active ingredient of the pesticide composition includes active ingredient A and active ingredient B, the active ingredient A is carmeconazole, and the active ingredient B is thifluzamide or fluazinam;The mass ratio of the active ingredient A to the active ingredient B is 1:45~40:1.The pesticide composition of the application has significant synergistic effect on target pathogenic fungi, can significantly improve the field efficacy, reduce the use amount of pesticide, and slow down the environmental pressure.

Description

Technical Field

[0001] This invention belongs to the field of pesticide sterilization technology and discloses a pesticide composition and its application. Background Technology

[0002] Rice is one of the most widely grown and consumed food crops in Asia. The losses caused by fungal diseases each year are a major factor restricting rice production. Rice sheath blight is one of them, causing a yield loss of about 25% in rice cultivation.

[0003] Rice sheath blight manifests as cloud-like lesions on the base of the stem, leaf sheaths, and panicles, severely impacting photosynthesis and nutrient transport, leading to empty grains, dead panicles, and whiteheads. In severe cases, large-scale lodging occurs, significantly reducing yield by 10% to 30%. Rice sheath blight is a fungal disease caused by *Rhizoctonia solani*. The pathogen overwinters mostly in the soil as sclerotia, but can also overwinter as mycelium on diseased plant debris and other host materials. It thrives under high temperature and humidity conditions. In recent years, due to improper fertilization and pesticide application, the affected area and severity of rice sheath blight have increased annually.

[0004] Currently, the control of rice sheath blight in production mainly relies on single agents such as thifluzamide. However, the long-term use of one or the same type of agent has accelerated the development of resistance and increased agricultural production costs. Research and screening have revealed highly efficient, safe, and broad-spectrum mixed fungicides that can effectively delay the development of resistance, significantly improve fungicidal activity, reduce pesticide usage, and lower costs. Summary of the Invention

[0005] Based on the above, the present invention provides a pesticide composition that rationally combines active compounds with different mechanisms of action, resulting in a significant synergistic effect against target pathogens, effectively improving field control efficacy, reducing pesticide dosage, and mitigating environmental pressure.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition, wherein the active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein active ingredient A is carmeconazole, and active ingredient B is thifluzamide or fluoxastrobin; the mass ratio of active ingredient A to active ingredient B is 1:45~40:1.

[0007] Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:18~20:1; The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:25~40:1.

[0008] Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 20:1; The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:18~20:1; Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 2:11 to 20:1; The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:12 to 5:1.

[0009] Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:18, 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:25, 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, 20:1, or 40:1.

[0010] Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, or 20:1. Furthermore, the active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:12, 1:8, 1:5, 7:6, or 5:1.

[0011] Furthermore, based on the total mass of the pesticide composition (100 wt%), the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5 to 80 wt%.

[0012] Furthermore, the pesticide composition, in addition to the active ingredient, also contains pesticide-acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0013] Furthermore, the pesticide composition is prepared as a solid formulation or a liquid formulation.

[0014] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, microemulsion, or dispersible oil suspension.

[0015] This invention also discloses the use of the pesticide composition described above in controlling plant pathogens, characterized in that the pathogen is *Rhizoctonia solani*. The beneficial effects of this invention are as follows: The pesticide composition of this invention rationally combines fungicidal active compounds with different mechanisms of action, which can produce significant synergistic effects, broaden the control spectrum, and effectively delay the emergence and development of pathogen resistance; After compounding compounds with different targets, it can interfere with the life activities of pathogens through multiple pathways, thereby improving the control effect and reliability; The pesticide composition of this invention reduces the application dosage of each single agent, which helps to reduce environmental pressure and production costs, and is an important strategy for achieving sustainable disease management. Detailed Implementation

[0016] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0017] The following is a preparation method for a formulation.

[0018] 1. Preparation method of water-dispersible granules: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet milling, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0019] 2. Preparation method of wettable powder: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a mixer. After being pulverized by an air jet mill, they are mixed evenly again to prepare the wettable powder of the composition of the present invention.

[0020] 3. Preparation method of emulsifiable concentrate: According to the formula ratio, add the measured active ingredients, solvent and co-solvent into the mixing tank and stir to dissolve them. Then add the emulsifier, and use the remaining solvent to make up the balance. Stir evenly in the mixing tank, and filter to obtain the emulsifiable concentrate required by the present invention.

[0021] 4. Preparation method of suspension: According to the formula ratio, place the active ingredients, surfactants and other functional additives in the reaction vessel in sequence, add water and mix evenly, then perform high-speed shearing, wet sand milling, and finally homogenization filtration to obtain the suspension product.

[0022] Formulation preparation example: Preparation Example 1: 26% carmeconazole·thifluzamide water-dispersible granules (2:11) Formula composition: 4% carmeconazole, 22% thifluzamide, 8% succinate sulfonate, 5% BX (a type of succinate), 5% sodium alkyl polyoxyethylene ether sulfonate, 12% white sugar, and kaolin to make up the balance.

[0023] Preparation Example 2: 30% carmeconazole·thifluzamide wettable powder (2:1) Formula composition: 20% carmeconazole, 10% thifluzamide, 10% sodium polycarboxylate, 5% fatty alcohol polyoxyethylene ether sulfate, 4% stretching powder BX, 10% silica, and kaolin to make up the balance.

[0024] Preparation Example 3: 11% carmeconazole·thifluzamide emulsifiable concentrate (8:3) Formula composition: 8% carmeconazole, 3% thifluzamide, 20% N-methylpyrrolidone, 10% glycerol fatty acid ester polyoxyethylene ether, 3% calcium dodecylbenzenesulfonate, 10% DMF, methyl oleate to make up the balance.

[0025] Preparation Example 4: 26% carmeconazole·thifluzamide suspension (11:2) Formula composition: 22% carmeconazole, 4% thifluzamide, 4% isotridecyl alcohol polyoxyethylene ether, 2% polyoxyethylene dehydrated sorbitan monooleate, 1.5% succinate sulfonate, 0.25% xanthan gum, 5% ethylene glycol, 0.3% magnesium aluminum silicate, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0026] Preparation Example 5: 32% carmeconazole·fluoxacillin water-dispersible granules (15:1) Formula composition: 30% carmeconazole, 2% fluoxetine, 5% sodium dodecyl sulfate, 10% sodium alkyl polyoxyethylene ether sulfonate, 4% sodium lignosulfonate, 10% ammonium sulfate, and starch to make up the balance.

[0027] Preparation Example 6: 26% carmeconazole·fluoxadiazon wettable powder (1:12) Formula composition: 2% carmeconazole, 24% fluoxetine, 8% calcium dodecylbenzenesulfonate, 5% sodium alkylnaphthalenesulfonate, 4% fatty alcohol polyoxyethylene ether sulfate, 5% silica, 18% starch, and kaolin to make up the balance.

[0028] Preparation Example 7: 6% carmeconazole·fluoxadiazon EC (1:5) Formula composition: 1% carmeconazole, 5% fluoxetine, 10% EO / PO block copolymer, 10% acetophenone, 5% propylene glycol methyl ether, 3% alkylphenol polyoxyethylene ether methyl ether condensate sodium sulfate, and tricresyl to make up the balance.

[0029] Preparation Example 8: 13% carmeconazole·fluoxadiazon suspension (7:6) Formula composition: 7% carmeconazole, 6% fluoxetine, 2% sorbitan oleate polyoxyethylene ether, 2% alkylphenol polyoxyethylene ether, 2% sodium alkyl polyoxyethylene ether sulfonate, 3% castor oil polyoxyethylene ether phosphate, 0.25% magnesium aluminum silicate, 0.15% carboxyethyl cellulose, 0.25% sodium sorbate, 5% glycerol, 0.5% silicone oil, deionized water to make up the balance.

[0030] Example 1: Indoor bioactivity assay.

[0031] Test basis: The test refers to NY / T 1156.5-2006 "Test Guidelines for Indoor Biological Activity Determination of Pesticides - Fungicides Part 5: Test for Inhibition of Rice Sheath Blight - Broad Bean Leaf Method".

[0032] Experimental target: Rice sheath blight pathogen ( Rhizoctonia solani ).

[0033] Test material preparation: Select disease-susceptible broad bean varieties, cut leaves from the same part, with uniform growth and petioles, place them in petri dishes, and keep them moist for later use.

[0034] Test agents: carmeconazole, thifluzamide, and fluoxastrobin technical grade.

[0035] Reagent preparation: Dissolve the test reagent in a suitable solvent, then dilute it with 0.05% Tween 80 aqueous solution. Based on the reagent activity, set 5 series of mass concentrations.

[0036] Chemical treatment: The leaves were fully immersed in the pre-prepared chemical solution for 5 seconds, the excess solution was drained off, and the leaves were air-dried naturally. After that, they were cultured in a moist environment according to the treatment label. The experiment set up a blank control with no chemical treatment.

[0037] Inoculation and cultivation: Using an inoculator, inoculate the mycelial side of a 5mm diameter mycelial cake onto the center of the treated leaf. Inoculate 30 leaves per treatment. After inoculation, place the leaves in a controlled climate chamber with light and cultivate them at a temperature of 26-28℃ and a relative humidity of 80%-90%.

[0038] Investigation: Based on the blank control invention, the length and width of the lesion at each inoculation point were measured and recorded using calipers, and the lesion diameter (unit: mm) was expressed as the average of the length and width.

[0039] Calculation method: Based on the survey data, calculate the prevention effect according to the following formula, with the unit being percentage (%), and the calculation result should be rounded to two decimal places.

[0040]

[0041] In the formula: P—Prevention and control effect; D0—Diameter of lesions in the blank control group; D1—Diameter of the lesion treated with the agent; Experimental statistics: The data was processed using probability value analysis. A statistical analysis system was used to analyze the data and calculate EC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0042] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0043] In the formula: ATI —Measured toxicity index of the mixture; S ——EC of standard fungicides 50 The unit is milligrams per liter (mg / L); M— —EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0044]

[0045] In the formula: TTI —Theoretical toxicity index of the mixture; TI A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0046]

[0047] In the formula: CTC —Cotoxicity coefficient; ATI —Measured toxicity index of the mixture; TTI —Theoretical toxicity index of mixed preparations.

[0048] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0049] The results of the indoor tests are shown below: Table 1. Results of indoor bioactivity tests of carmeconazole and thifluzamide combination against rice sheath blight. carmeconazole (A) / / y=3.5214+1.7698x 0.9988 6.8463 / thifluzamide (B) / / y=4.3642+1.7522x 0.9942 2.3058 / A:B 1 30 y=4.4075+1.6992x 0.9946 2.2319 105.570 A:B 1 18 y=4.4898+1.7775x 0.9956 1.9365 123.377 A:B 1 10 y=4.5185+1.8019x 0.9882 1.8502 132.620 A:B 2 11 y=4.5066+2.0077x 0.9944 1.7610 145.815 A:B 3 8 y=4.5291+2.0229x 0.9947 1.7091 164.704 A:B 2 1 y=4.6174+1.9234x 0.9925 1.5810 261.434 A:B 8 3 y=4.3762+1.7651x 0.9965 2.2564 197.403 A:B 11 2 y=4.3408+1.3943x 0.9991 2.9704 176.894 A:B 10 1 y=4.1832+1.4003x 0.9988 3.8313 151.562 A:B 20 1 y=4.0866+1.4677x 0.9988 4.1914 149.338 A:B 30 1 y=3.9479+1.4185x 0.9919 5.5169 116.685 Indoor experiments showed that combining carmeconazole and thifluzamide in an appropriate mass ratio effectively controlled rice sheath blight. A mass ratio of 1:18–20:1 resulted in a co-toxicity coefficient greater than 120, indicating a synergistic effect; a ratio of 1:10–20:1 resulted in a co-toxicity coefficient greater than 130, showing a significant synergistic effect; and a ratio of 2:11–20:1 resulted in a co-toxicity coefficient greater than 140, also demonstrating a remarkable synergistic effect. Among them, the mass ratios of carmeconazole to thifluzamide of 1:18, 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, and 20:1 showed a co-toxicity coefficient greater than 120, indicating a synergistic effect; the mass ratios of carmeconazole to thifluzamide of 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, and 20:1 showed a co-toxicity coefficient greater than 130, indicating a significant synergistic effect; and the mass ratios of carmeconazole to thifluzamide of 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, and 20:1 showed a co-toxicity coefficient greater than 140, indicating a significant synergistic effect.

[0050] Table 2 Results of indoor bioactivity tests of carmeconazole and fluopyram combined on rice sheath blight. carmeconazole (A) / / y=3.8781+1.3446x 0.9975 6.8288 / sedaxane (B) / / y=4.2576+1.4939x 0.9999 3.1402 / A:B 1 40 y=4.3197+1.4679x 0.9974 2.9070 109.464 A:B 1 25 y=4.3803+1.4778x 0.9963 2.6263 122.104 A:B 1 18 y=4.4376+1.4968x 0.9948 2.3754 136.065 A:B 1 12 y=4.4918+1.5263x 0.9927 2.1525 152.211 A:B 1 8 y=4.5427+1.5694x 0.9898 1.9560 170.792 A:B 1 5 y=4.5502+1.6261x 0.9909 1.8906 182.528 A:B 7 6 y=4.4350+1.4743x 0.9978 2.4166 183.238 A:B 5 1 y=4.1786+1.4666x 0.9988 3.6315 157.257 A:B 10 1 y=4.0208+1.5151x 0.9974 4.4288 139.314 A:B 20 1 y=3.9293+1.5572x 0.9959 4.8704 132.783 A:B 40 1 y=3.8248+1.6167x 0.9934 5.3325 124.493 Indoor experiments showed that combining carmeconazole and fluopyram at an appropriate mass ratio effectively controlled rice sheath blight. A mass ratio of 1:25–40:1 resulted in a co-toxicity coefficient greater than 120, indicating a synergistic effect; a ratio of 1:18–20:1 resulted in a co-toxicity coefficient greater than 130, showing a significant synergistic effect; and a ratio of 1:12–5:1 resulted in a co-toxicity coefficient greater than 140, also indicating a remarkable synergistic effect. Among them, the mass ratios of carmeconazole and fluoxetine were 1:25, 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, 20:1, and 40:1, with a co-toxicity coefficient greater than 120, showing a synergistic effect; the mass ratios of 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, and 20:1, with a co-toxicity coefficient greater than 130, showing a significant synergistic effect; and the mass ratios of 1:12, 1:8, 1:5, 7:6, and 5:1, with a co-toxicity coefficient greater than 140, showing a significant synergistic effect.

[0051] Example 2: Field efficacy test for controlling rice sheath blight.

[0052] Experimental location: The experiment was conducted in Xiazhen Village, Wan'an Town, Wuping County, Fujian Province. The experimental fields were flat, with convenient irrigation and drainage, moderate fertility, and basically consistent field management levels.

[0053] Experimental target: Rice sheath blight.

[0054] Experimental Design: The experiment consisted of 6 treatments, each replicated 4 times, with a plot area of ​​30m². 2 The rice plants were randomly assigned to blocks. Each treatment used 450 kg / ha of water to ensure uniform spraying of the rice leaves.

[0055] Experimental Methods: The experimental survey was conducted in accordance with GB / T 17980.20—2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Rice Sheath Blight with Fungicides". The experiment was conducted with the first application on September 6, 2025, and the second application on September 13, 2025. Two surveys were conducted: one 7 days after the first application and another 10 days after the second application. Five points were sampled diagonally from each plot, and four adjacent rice clumps were surveyed at each point. The total number of plants, the number of diseased plants, the disease severity, and the effects of the application on rice and other organisms were recorded. The disease index and control effect were calculated. The disease grading criteria are shown below: The disease grading criteria are as follows: Grade 0: The entire plant is disease-free; Grade 1: Disease occurs on the fourth leaf and all leaf sheaths and leaves below (with the top leaf considered as the first leaf); Grade 3: Disease occurs in the third leaf and all leaf sheaths and leaves below it; Level 5: Disease occurs on the second leaf and all subsequent leaf sheaths and leaves; Level 7: Disease occurs on the sword leaf and all leaf sheaths and leaves below it; Level 9: The entire plant is infected and dies prematurely.

[0056]

[0057] Table 3 Results of field efficacy trials for controlling rice sheath blight

[0058] Field efficacy trials showed that no adverse effects of the test pesticides on rice or other organisms were observed during the experiment. Five pesticide treatments were used to prepare Example 2: 30% carmeconazole·thifluzamide wettable powder (2:1) 80 g / hm². 2 It showed the best control efficacy against rice sheath blight, with a disease index control rate of 88.55% 10 days after the second application; Preparation Example 7: 6% carmeconazole·fluoxadiazon EC (1:5) 80g / hm 2 The control efficacy against rice sheath blight was 85.30%.

Claims

1. A pesticide composition, characterized in that, The active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein active ingredient A is carmeconazole and active ingredient B is thifluzamide or fluoxastrobin; the mass ratio of active ingredient A to active ingredient B is 1:45 to 40:

1.

2. The pesticide composition according to claim 1, characterized in that, The active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:18~20:1; The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:25~40:

1.

3. The pesticide composition according to claim 2, characterized in that, The active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 20:1; The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:18~20:1; Preferably, the active ingredient B is thifluzamide, and the mass ratio of the active ingredient A to the active ingredient B is 2:11 to 20:

1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:12 to 5:

1.

4. The pesticide composition according to claim 1, characterized in that, The active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:18, 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:

1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:25, 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, 20:1, or 40:

1.

5. The pesticide composition according to claim 4, characterized in that, The active ingredient B is thifluzamide, and the mass ratio of active ingredient A to active ingredient B is 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:

1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:18, 1:12, 1:8, 1:5, 7:6, 5:1, 10:1, or 20:

1. Preferably, the active ingredient B is thifluzamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:10, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, or 20:

1. The active ingredient B is fluoxastrobin, and the mass ratio of active ingredient A to active ingredient B is 1:12, 1:8, 1:5, 7:6, or 5:

1.

6. The pesticide composition according to claim 1, characterized in that, Based on the total mass of the pesticide composition, which is 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5 to 80 wt%.

7. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition contains pesticide-acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

8. The pesticide composition according to claim 1, characterized in that, The pesticide composition is prepared as a solid or liquid formulation.

9. The pesticide composition according to claim 6, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, microemulsion, or dispersible oil suspension.

10. The use of the pesticide composition according to any one of claims 1-9 in the control of plant pathogens, characterized in that, The pathogen mentioned is rice sheath blight pathogen.

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