A hair growth composition with multiple synergistic effects and a preparation method thereof

Abstract

This invention discloses a hair growth composition with multiple synergistic effects and its preparation method, belonging to the field of hair growth cosmetics. The composition comprises the following components in parts by weight: 7.5-10 parts sodium lauryl sulfate, 3.2-5.3 parts camel thorn leaf extract, 3.6-6.4 parts macadamia nut oil, 0.3-1 part Selaginella tamariscina extract, 0.3-1 part black pepper extract, 0.5-1.1 parts green coffee bean extract, 0.5-1.3 parts rosehip oil, 0.01-0.1 parts copper peptide, and 0.5-1.5 parts coenzyme Q10. 10 The composition comprises 3-10 parts by weight of copidrometerium, 0.5-5 parts by weight of amnesic acid, 1.5-3.5 parts by weight of adenosine, 2.2-3.3 parts by weight of nicotinamide, 0.3-1 part by weight of sphingomyelin, 0.5-0.8 parts by weight of preservative, 27-38 parts by weight of penetration enhancer, and 26-48.59 parts by weight of buffer solution. All plant extracts are separated using nanofiltration membranes. The prepared composition exhibits multiple synergistic effects, achieving highly efficient hair growth.

Description

Technical Field

[0001] This invention belongs to the field of hair growth and hair development cosmetics, specifically relating to a hair growth and hair development composition with multiple synergistic effects and its preparation method. Background Technology

[0002] Hair loss in China has now transformed from a problem for middle-aged and elderly people into a health concern for the entire population, exhibiting four core characteristics: large scale, younger age of onset, higher incidence among men, and predominance of androgenetic alopecia.

[0003] Currently, methods to address hair regrowth generally involve regulating single or multiple networks within the hair follicle system, such as improving the follicle environment to activate hair follicles, increasing transdermal penetration, and increasing the number of target points. For example, patent CN118512561A addresses hair follicle regrowth by improving the follicle environment from multiple angles to activate hair follicles; patent CN118178293A increases the penetration of active ingredients in the formula, preventing hair follicle aging and damage, and providing nutrition; and patent US11312714B2 discloses a mitochondrial pyruvate carrier inhibitor that can promote hair growth. However, the skin permeability of active ingredients remains a significant problem, with extremely low transdermal penetration and low hair regrowth efficiency. Patent CN115844984A discloses a method to increase minoxidil absorption; and patent CN113827608A discloses a method to rapidly activate minoxidil. Current methods to increase the transdermal penetration of active ingredients include enhancing solvent permeability, microneedle penetration, and liposomes. Enhancing the skin's permeability and microneedling methods both cause significant skin damage and increase sensitivity; liposomes are also expensive. New methods to improve hair growth and development are urgently needed.

[0004] Therefore, there is a need to develop a hair growth composition with multiple synergistic effects to improve hair growth efficiency, and in particular, to provide a hair growth composition with multiple synergistic effects to solve the problems existing in the prior art. Summary of the Invention

[0005] To address the aforementioned background, this invention provides a hair growth composition with multiple synergistic effects and its preparation method. Sodium lauryl sulfate, camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and sphingomyelin in the composition synergistically promote hair growth. The prepared composition stimulates the skin to secrete monounsaturated fatty acids through micro-damage, thereby activating hair follicle stem cells. The composition simultaneously performs micro-damage and repair, both activating hair follicle stem cells through micro-damage and maintaining hair follicle homeostasis. The composition achieves anti-inflammatory and repair effects through the presence of anti-inflammatory active ingredients, and regulates cell proliferation and anti-aging / anti-inflammatory effects through ceramides generated from sphingomyelin decomposition. The composition activates hair follicle stem cells through micro-damage and also through free monounsaturated fatty acids and β-carotene. Simultaneously, nanofiltration technology reduces the molecular weight of compounds in the plant extracts, improving the skin permeability of the plant extracts and enhancing the efficacy of the composition, achieving a more efficient hair growth effect. The added copper peptide promotes skin absorption and simultaneously enhances the hair growth efficacy of the composition. Nanoscale emulsions are prepared through emulsification, further enhancing the skin permeability of the composition. Multiple synergistic effects result in highly efficient hair growth.

[0006] This invention is achieved through the following technical solution:

[0007] This invention provides a hair growth composition with multiple synergistic effects, the composition comprising the following components in parts by weight: 7.5-10 parts by weight of sodium dodecyl sulfate, 8.4-16.1 parts by weight of plant extract, 0.01-0.1 parts by weight of copper peptide, and 0.5-1.5 parts by weight of coenzyme Q. 10 The plant extract comprises the following components by weight: 3-10 parts copidrome, 0.5-5 parts ammonium chloride, 1.5-3.5 parts adenosine, 2.2-3.3 parts nicotinamide, 0.3-1 parts sphingomyelin, 0.5-0.8 parts preservative, 27-38 parts permeabilizer, and 26-48.59 parts buffer solution; the plant extract further comprises the following components by weight: 3.2-5.3 parts camel thorn leaf extract, 3.6-6.4 parts macadamia nut oil, 0.3-1 parts selaginella extract, 0.3-1 parts black pepper extract, 0.5-1.1 parts green coffee bean extract, and 0.5-1.3 parts rosehip oil; the plant extract is subjected to nanofiltration separation.

[0008] In this invention, the components of the composition are selected within a certain range, so that the total amount of the composition is 100 parts by weight. Sodium dodecyl sulfate (SLS) comprises 7.5-10 parts by weight in the total composition, accounting for 7.5-10%. The presence of 7.5-10% SLS in the composition can cause minor skin damage, leading to mild inflammation. After the skin damage triggers local inflammation, reversible lipolysis occurs in the dermal fat cells, resulting in a decrease in triglycerides and an increase in the release of free monounsaturated fatty acids. Monounsaturated fatty acids can activate hair follicle stem cells, thereby achieving the effect of hair regrowth.

[0009] In this invention, the preparation steps of the plant extract are as follows:

[0010] After crushing the plant, extractant A is added, followed by microwave treatment or pressing to obtain plant extract; the plant extract is then subjected to nanofiltration to obtain plant isolate; the plant isolate is concentrated by rotary evaporation or high-speed centrifugation, and then dried to obtain plant extract.

[0011] The nanofiltration separation process includes the following steps:

[0012] The plant extract was filtered through a 50-200 mesh metal filter, and then through a 5-micron polypropylene membrane to obtain filtrate 1. Filtrate 1 was then filtered through a 0.1-1 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2. Filtrate 2 was then filtered through a 0.01-0.05 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3. Filtrate 3 was then filtered through a 0.66 nanometer polyvinylidene fluoride membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is the plant isolate. The metal filter was selected from copper, silver, and stainless steel.

[0013] The plants mentioned include camel thorn leaves, macadamia nuts, selaginella, black pepper, green coffee beans, and rosehips; the extractant A is selected from one or more of ethanol, diethyl ether, ethyl acetate, propylene glycol, and butylene glycol; the microwave treatment is performed by using microwaves at 2300-2500 MHz at 50-55 degrees Celsius for 15-25 minutes; the pressing treatment is performed by using a hydraulic press at 45-55 degrees Celsius for 0.5-1 hours; the rotary evaporation concentration is performed by rotary evaporation at 45-55 degrees Celsius and -1 to -0.9 MPa for 5-8 hours; the high-speed centrifugal concentration is performed by centrifuging at 10000-13000 r / min for 1-1.5 hours; and the drying is performed by drying at 50-55 degrees Celsius for 72-80 hours.

[0014] The compounds in plant extracts are separated into the extraction agent more efficiently by microwave or pressing methods, improving industrial efficiency. The plant extract obtained in the above steps contains a large number of plant solid particles. The plant extract is filtered through a 50-200 mesh metal filter and then through a 5-micron polypropylene filter membrane. This separation process removes broken plant solid particles. Filtrate 1 is then filtered through a 0.1-1 micron polyvinylidene fluoride filter membrane or an inorganic ceramic filter membrane. This separation process further removes solid particles larger than 0.1-1 microns. Filtrate 2 is then filtered through a 0.01-0.05 micron polyvinylidene fluoride filter membrane or an inorganic ceramic filter membrane. This separation process removes macromolecules larger than 0.01-0.05 microns, including crude fiber, crude protein, crude fat, and polysaccharides. Filtrate 3 is then filtered through a 0.66-nanometer polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane. This separation process removes molecules with a molecular weight of approximately 1000 Da or higher. After concentration and drying, the plant extract is obtained. Furthermore, substances with a molecular weight of less than 500 Da can easily penetrate the stratum corneum of the skin, making the plant extracts obtained by this separation method more efficient at skin penetration.

[0015] The crude fiber, crude protein, crude fat, and polysaccharides in camel thorn leaves were separated by microwave-membrane separation. The camel thorn leaf extract obtained by microwave-membrane separation contains flavonoids, alkaloids, polyphenols, tannins, amino acids, and minerals. These substances also possess anti-inflammatory and antioxidant effects. Camel thorn leaf extract obtained by conventional alcohol extraction contains crude fiber, crude protein, crude fat, and polysaccharides in high proportions. Furthermore, these substances hinder skin permeation, preventing small molecules from passing through the stratum corneum. Compared to camel thorn leaf extract prepared by alcohol extraction, the camel thorn leaf extract prepared by microwave-membrane separation has improved skin permeability of its active ingredients, significantly enhancing the anti-inflammatory and antioxidant capabilities of the composition. Macadamia oil obtained by pressing-membrane separation contains flavonoids, tannins, polyphenols, oleic acid, and palmitoleic acid. Macadamia prepared by this method can significantly increase the probability of monounsaturated fatty acids entering the dermis, increase the ability to activate hair follicle stem cells, and at the same time have anti-inflammatory and antioxidant capabilities, thus improving hair growth and development.

[0016] The *Selaginella tamariscina* extract obtained by microwave-membrane separation contains *Tamarix chinensis* flavonoids and dihydromyricetin. *Tamarix chinensis* flavonoids possess antioxidant, anti-inflammatory, melanin-inhibiting, soothing, and penetration-enhancing effects, while dihydromyricetin exhibits a novel, mild penetration-enhancing effect, combining anti-inflammatory and antioxidant properties, and is non-irritating, making it a penetration enhancer specifically for sensitive skin. The black pepper extract obtained by microwave-membrane separation contains piperine, α-phellandrene, flavonoids, phenolic acids, sterols, manganese, and zinc. Piperine and α-phellandrene enhance the permeation of polyphenols through the skin. The green coffee bean extract obtained by microwave-membrane separation contains chlorogenic acid. Chlorogenic acid possesses potent anti-inflammatory and antioxidant effects. The rosehip oil obtained by pressing-membrane separation contains oleic acid, linoleic acid, α-linolenic acid, palmitic acid, stearic acid, trace amounts of phytosterols, and trace amounts of β-carotene. Substances with a molecular weight less than 500 Da easily penetrate the stratum corneum of the skin. Beta-carotene is a provitamin A, which is enzymatically hydrolyzed in the skin to produce retinol, activating hair follicle stem cells. The retinol produced from a small amount of beta-carotene through enzymatic hydrolysis in the skin is within the regulated usage limits. Oleic acid is a free monounsaturated fatty acid, which can activate hair follicles and enhance hair growth. Linoleic acid can repair the skin barrier and synthesize ceramides; alpha-linolenic acid has a strong anti-inflammatory effect and soothes redness; phytosterols have repairing and soothing effects on sensitivity; beta-carotene activates hair follicle stem cells, promotes absorption, and directly protects hair follicle cells.

[0017] Camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, and rosehip oil, extracted using conventional alcohol extraction methods, contain high proportions of crude fiber, crude protein, crude fat, and polysaccharides. These crude fibers, proteins, fats, and polysaccharides hinder skin permeation, preventing small molecules from passing through the stratum corneum and reducing the efficiency of small molecule permeation, thus weakening the hair growth efficacy of the small molecule active ingredients in the composition. In this invention, plant extracts prepared using microwave-membrane separation or pressing-membrane separation methods have molecular weights greater than 1000 Da filtered out, while substances with molecular weights less than 500 Da easily pass through the stratum corneum. This indicates that nanofiltration controls the molecular weight of compounds in the plant extracts, enhancing the permeability of the active ingredients and thus achieving a highly effective hair growth effect.

[0018] In this invention, the sphingomyelin, upon entering the stratum corneum and dermis, decomposes into ceramides. Ceramides strengthen the skin barrier, reduce transepidermal water loss, and soothe dry and sensitive skin. The added camel thorn leaf extract contains flavonoids, alkaloids, polyphenols, tannins, amino acids, and minerals, which have anti-inflammatory and oxidative damage repair effects, thus repairing the skin. The added macadamia nut oil contains flavonoids, tannins, and polyphenols, which have anti-inflammatory and antioxidant effects, also contributing to skin repair. The added Selaginella extract contains paclitaxel biflavonoids and dihydromyricetin. Paprika biflavonoids have antioxidant, anti-inflammatory, melanin-inhibiting, soothing, and penetration-promoting effects, also contributing to skin repair. The added green coffee bean contains chlorogenic acid, which has potent anti-inflammatory and antioxidant effects, also contributing to skin repair. This indicates that the composition repairs the skin by containing effective anti-inflammatory ingredients and regulates cell proliferation, anti-aging, and anti-inflammatory effects through the ceramides generated from the decomposition of sphingomyelin. It is also explained that the composition can simultaneously perform micro-damage and repair, which can both activate hair follicle stem cells through micro-damage to achieve efficient hair growth and maintain the homeostasis of hair follicles. The two effects work synergistically to obtain excellent hair growth results.

[0019] The addition of sphingomyelin forms a nano-emulsion during emulsification. Nano-sized sphingomyelin penetrates the skin's stratum corneum more easily, enhancing the skin's permeability of the active ingredients and thus improving hair growth. The composition uses nanofiltration to control the molecular weight of compounds in the plant extracts, further enhancing the skin's permeability of the active ingredients and achieving a highly effective hair growth effect. The composition also enhances hair growth by activating hair follicles through micro-damage. These three actions work synergistically to achieve a more efficient hair growth effect, overcoming the impact of low skin permeability of the active ingredients.

[0020] Macadamia nuts contain oleic acid and palmitoleic acid, both of which are monounsaturated fatty acids. Oleic and palmitoleic acids can penetrate the dermis and activate hair follicle stem cells, thus promoting hair growth. A 7.5-10% concentration of sodium lauryl sulfate induces mild inflammation through micro-injury, promoting the secretion of monounsaturated fatty acids, thereby activating hair follicle stem cells and achieving hair growth. The macadamia nuts in the composition contain oleic and palmitoleic acids, which, being monounsaturated acids, can penetrate the dermis through the skin to directly activate hair follicle stem cells, achieving hair growth. Simultaneously, the added rosehip oil contains β-carotene, which activates hair follicle stem cells, promotes absorption, and directly protects hair follicle cells. This indicates that the 7.5-10% concentration of sodium lauryl sulfate, macadamia nut oil, and rosehip oil in the composition can both activate hair follicle stem cells through micro-injury and activate and protect them through the addition of free monounsaturated fatty acids and β-carotene, achieving a highly effective hair growth effect. These two synergistic effects result in a stronger hair growth outcome. Furthermore, the composition not only effectively activates hair follicle stem cells, but also protects hair follicles.

[0021] The added Selaginella extract contains paclitaxel flavonoids and dihydromyricetin. Dihydromyricetin has a novel, gentle penetration-enhancing effect, combining anti-inflammatory and antioxidant properties, and is non-irritating, making it a penetration enhancer specifically for sensitive skin. The added black pepper extract contains piperine, which enhances the transdermal absorption of polyphenols. The added copper peptide promotes transdermal absorption. This indicates that the dihydromyricetin, piperine, and copper peptide in the composition can enhance the transdermal absorption of the active ingredients.

[0022] In this invention, the composition contains coenzyme Q. 10 Copinotone, Amenosides, adenosine, and niacinamide provide energy, increase the resilience of hair follicle blood vessels, improve metabolism, and protect nerves during hair growth, making the hair growth process more efficient. In this composition, sodium lauryl sulfate, macadamia nut oil, and rosehip oil activate hair follicle stem cells through two pathways: activating them through micro-damage and activating and protecting them through free monounsaturated fatty acids and β-carotene. Sodium lauryl sulfate, camel thorn leaf extract, selaginella extract, sphingomyelin, green coffee bean extract, macadamia nut oil, and rosehip oil work together to simultaneously promote hair growth through micro-damage and repair, achieving hair growth through damage while maintaining hair follicle homeostasis. Blue copper peptide, black pepper extract, and selaginella extract promote transdermal absorption of the active ingredients for hair growth. This multi-synergistic effect results in highly efficient hair growth.

[0023] In this invention, the composition simultaneously performs micro-damage and repair, which can both activate hair follicle stem cells through micro-damage to achieve highly efficient hair growth and maintain hair follicle homeostasis. The composition activates hair follicle stem cells through micro-damage, while free monounsaturated fatty acids and β-carotene simultaneously activate and protect them, thus activating hair follicle stem cells through two pathways. The dihydromyricetin, piperine, and copper peptide contained in the composition can enhance the skin penetration of the active ingredients. Nanofiltration controls the molecular weight of compounds in the plant extracts, enhancing the skin penetration ability of the hair growth active ingredients. This invention, through multiple synergistic effects, provides a new approach to overcome the industry problem of low skin penetration efficiency and poor efficacy of hair growth active ingredients.

[0024] In this invention, the preservative is one or more selected from phenoxyethanol, ethylhexylglycerin, and ethanol. Phenoxyethanol and ethylhexylglycerin are preferred preservatives. The penetrant is selected from one or more selected from ethanol, propylene glycol, butylene glycol, and PEG-40. Ethanol and propylene glycol are preferred penetrants, and ethanol and butylene glycol are also preferred penetrants. The buffer solution is a mixture of sodium citrate and citric acid; wherein the weight ratio of citric acid to sodium citrate in the buffer solution is 1.1-11.5:1.

[0025] This invention provides a method for preparing a hair growth composition with multiple synergistic effects, comprising the following steps:

[0026] Preparation of the alcohol phase liquid: 3.2-5.3 parts by weight of camel thorn leaf extract, 0.3-1 parts by weight of selaginella extract, 0.3-1 parts by weight of black pepper extract, 0.5-1.1 parts by weight of green coffee bean extract, 0.5-1.3 parts by weight of rosehip oil, 1.5-5 parts by weight of copiolite, and 0.3-1 parts by weight of sphingomyelin were placed in 27-38 parts by weight of permeabilizer and stirred at 45-57 degrees Celsius for 0.5-1 h to obtain the alcohol phase liquid;

[0027] Preparation of aqueous phase liquid: 7.5-10 parts by weight of sodium dodecyl sulfate, 0.5-5 parts by weight of amnesic acid, 1.5-3.5 parts by weight of adenosine, 2.2-3.3 parts by weight of nicotinamide, and 0.5-0.8 parts by weight of preservative are placed in 26-48.59 parts by weight of buffer solution and stirred at 60-70 degrees Celsius for 0.5-1 h. Then, 1.5-5 parts by weight of cobilol are added and stirred for 0.6-0.8 h to obtain aqueous phase liquid.

[0028] Preparation of the hair growth and development composition: While stirring at 800-2000 r / min, inject the alcohol phase liquid (45-57°C) into the aqueous phase liquid (60-70°C), emulsify at 6000-7000 r / min for 5-10 min, then place at 45-55°C and stir at 800-2000 r / min for 0.5-1 h. Next, add 3.6-6.4 parts by weight of macadamia nut oil and 0.5-1.5 parts by weight of coenzyme Q10. 10 Mix 0.01-0.1 parts by weight of blue copper peptide and stir at 45-55 degrees Celsius for 0.5-1 hours to obtain a hair growth composition.

[0029] In this invention, cobiliol is added in two steps, totaling 3-10 parts by weight. This two-step addition results in superior dispersibility, ensuring stable dispersion of cobiliol in the composition. Furthermore, the plant extracts prepared by nanofiltration and the liposomes prepared by emulsification enhance the skin permeability of the hair growth and development active ingredients in the composition. Different plant extracts are added step-by-step during the composition preparation process.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) In this invention, crude fiber, crude protein, crude fat, and polysaccharides in camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, and rosehip oil are filtered and separated by microwave-membrane separation or pressing-membrane separation. In the extracts obtained by microwave-membrane separation or pressing-membrane separation, molecules with a molecular weight greater than 1000 Da are filtered out, while substances with a molecular weight less than 500 Da easily pass through the stratum corneum of the skin. Nanofiltration controls the molecular weight of compounds in the plant extracts in the composition, enhancing the skin permeability of the active ingredients in the composition, thereby achieving a highly effective hair growth effect. The added Selaginella extract contains Taxodium biflavonoids and dihydromyricetin. Dihydromyricetin has a novel, mild penetration-enhancing effect, combining anti-inflammatory and antioxidant properties, and is non-irritating, making it a penetration enhancer specifically for sensitive skin. The added black pepper extract contains piperine, which enhances the transdermal absorption of polyphenols. The added copper peptide promotes transdermal absorption. This indicates that the dihydromyricetin, piperine, and copper peptide in the composition can enhance the transdermal absorption of the active ingredients. The added sphingomyelin forms a nano-emulsion during emulsification. Nano-sphingomyelin more easily penetrates the stratum corneum, and this nano-emulsion enhances the transdermal absorption of the active ingredients, thus improving hair growth. These three ingredients work synergistically to achieve highly effective hair growth.

[0032] (2) In this invention, 7.5-10% sodium lauryl sulfate can cause minor skin damage and mild inflammation. After the skin damage triggers local inflammation, the dermal fat cells undergo reversible lipolysis, resulting in a decrease in triglycerides and an increase in the release of free monounsaturated fatty acids. Monounsaturated fatty acids can activate hair follicle stem cells, thereby achieving the effect of hair growth. This overcomes the effect of low skin penetration efficiency of the active ingredients. When sphingomyelin enters the stratum corneum and dermis, it can decompose into ceramides. Ceramides can strengthen the skin barrier, reduce transepidermal water loss, and soothe dry and sensitive skin. The added camel thorn leaf extract contains flavonoids, alkaloids, polyphenols, tannins, amino acids, and minerals, which can have anti-inflammatory and oxidative damage repair effects, thus achieving the effect of skin repair. The added macadamia nut oil contains flavonoids, tannins, and polyphenols, which have anti-inflammatory and antioxidant effects, and can also achieve the effect of skin repair. The added selaginella extract contains paclitaxel biflavonoids and dihydromyricetin. Pyracantha biflavonoids have antioxidant, anti-inflammatory, melanin-inhibiting, soothing, and penetration-promoting effects, and can also achieve the effect of skin repair. The added green coffee beans contain chlorogenic acid, which has powerful anti-inflammatory and antioxidant effects, and can also repair the skin. The composition repairs the skin by containing effective anti-inflammatory ingredients, and regulates cell proliferation, anti-aging, and anti-inflammatory effects through ceramides generated from the decomposition of sphingomyelin. The composition achieves simultaneous micro-damage and repair, which can activate hair follicle stem cells through micro-damage to achieve highly efficient hair growth, and also maintain the homeostasis of hair follicles.

[0033] (3) In this invention, sodium lauryl sulfate (7.5-10%) induces mild inflammation through micro-injury, promoting the secretion of monounsaturated fatty acids, thereby activating hair follicle stem cells and achieving hair growth. Macadamia nuts added to the composition contain oleic acid and palmitoleic acid, both of which are monounsaturated acids. These acids penetrate the skin and directly activate hair follicle stem cells in the dermis, achieving hair growth. Simultaneously, rosehip oil contains β-carotene, which activates hair follicle stem cells, promotes absorption, and directly protects hair follicle cells. The 7.5-10% sodium lauryl sulfate, macadamia nut oil, and rosehip oil in the composition can both activate hair follicle stem cells through micro-injury and activate and protect them through the added monounsaturated fatty acids and β-carotene, achieving a highly effective hair growth effect. Furthermore, the composition effectively activates hair follicle stem cells while also protecting them.

[0034] (4) The prepared composition achieves simultaneous micro-damage and repair, which can both activate hair follicle stem cells through micro-damage and maintain hair follicle homeostasis. The two effects work synergistically to obtain better hair growth effect. The composition achieves anti-inflammatory and repair effects by containing anti-inflammatory active ingredients, and regulates cell proliferation, anti-aging, and anti-inflammation through ceramides generated by sphingomyelin decomposition. The two effects work synergistically to obtain strong hair growth effect. The composition activates hair follicle stem cells through micro-damage, and activates and protects hair follicle stem cells through free monounsaturated fatty acids and β-carotene. The two effects work synergistically to enhance hair growth effect. The addition of sphingomyelin forms a nano-emulsion during emulsification. Nano-sized sphingomyelin more easily penetrates the stratum corneum of the skin, and this nano-emulsion enhances the skin permeability of the active ingredients, thus improving hair growth. The composition controls the molecular weight of compounds in the plant extracts through nanofiltration, further enhancing the skin permeability of the active ingredients and achieving a highly effective hair growth effect. The composition also enhances hair growth by activating hair follicles through micro-damage. Dihydromyricetin, piperine, and copper peptides in the composition further enhance the skin permeability of the active ingredients. These combined effects work synergistically to achieve excellent hair growth results. This invention, through multiple synergistic effects, provides a new approach to overcome the industry problem of low skin permeability and poor efficacy of hair growth active ingredients.

[0035] These and other features and advantages of the present invention will become apparent from the following description of the embodiments and examples. Attached Figure Description

[0036] Figure 1 The expression of 5α-reductase (SRD5A2) in mouse skin tissue was detected by Western blot (WB) in the control group, model group, positive group and sample of Example 1 in the animal experiment of the example.

[0037] Figure 2 The number of terminal hairs (coarse) in the control group, model group, positive group, and sample of Example 1 in the animal experiment of the example is counted.

[0038] Figure 3 The number of fine vellus hairs was counted in the control group, model group, positive group, and sample of Example 1 in the animal experiment of the example.

[0039] Figure 4 HE staining was performed on day 21 of the control group, model group, positive group, and sample from Example 1 in the animal experiment to detect the pathological expression and hair follicle status of mouse skin tissue.

[0040] Figure 5 For the animal experiments of the example, the growth of hair on the back of mice was recorded by photograph on days 1, 7, 14, and 21 of the control group, model group, positive group, and sample of Example 1.

[0041] Figure 6 This is a particle size distribution of the sample from Example 1.

[0042] Figure 7 This is the Tyndall effect observed in the sample of Example 1 under infrared laser light.

[0043] Figure 8 This is a photograph of the actual sample from Example 1.

[0044] Figure 9 This is the phenomenon of infrared laser light passing through the sample of Comparative Example 11.

[0045] Figure 10 The growth of hair on the back of mice was recorded by photographing the samples from Example 1 on days 1-21 in the animal experiments of Example 1.

[0046] Figure 11 The expression of 5α-reductase (SRD5A2) in mouse skin tissue of the control group, model group, positive group and sample of Example 1 in the animal experiment of the example is shown.

[0047] Figure 12 The expression of 5α-reductase (SRD5A2) in mouse skin tissue of the control group, model group, positive group and sample of Example 1 in the animal experiment of the example is shown.

[0048] Figure 13 This is a comparison image of the sample from Example 1 used in human trials before and after 12 weeks.

[0049] Figure 14 This is a comparison image of the sample from Example 1 used in human trials before and after 12 weeks.

[0050] Figure 15This is a comparison image of the sample from Example 1 used in human trials before and after 12 weeks.

[0051] Figure 16 HE staining was performed on day 21 of the control group, model group, positive group, and sample from Example 1 in the animal experiment to detect the pathological expression and hair follicle status of mouse skin tissue.

[0052] Figure 17 HE staining was performed on day 21 of the control group, model group, positive group, and sample from Example 1 in the animal experiment to detect the pathological expression and hair follicle status of mouse skin tissue. Detailed Implementation

[0053] Terminology Explanation

[0054] In this invention, "ethanol" refers to di-dealdehyde ethanol.

[0055] In this invention, "Coenzyme Q" 10 "It is a coenzyme found in all eukaryotes that perform aerobic respiration."

[0056] In this invention, "combirol" refers to pyrrolidinyl diaminopyrimidine oxide.

[0057] In this invention, "Amenes" refers to diaminopyrimidine oxide.

[0058] In this invention, "peg-40" refers to polyoxyethylene 40 hydrogenated castor oil.

[0059] In this invention, "water" refers to distilled water.

[0060] In this invention, the "emulsion" is a liposome emulsion, which is a colloidal dispersion emulsion system formed by uniformly dispersing phospholipid bilayer vesicles in an aqueous continuous matrix.

[0061] In this invention, "0.66 nm polyvinylidene fluoride filter membrane or inorganic ceramic nanofiltration membrane" refers to the nominal PEG diameter of the industrial membrane, and 0.66 nm corresponds to a molecular weight of approximately 1000 Da.

[0062] In this invention, "PBS solution" refers to a solution prepared by disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride and other components in a specific ratio, with a pH value usually maintained at 7.2-7.4 and an osmotic pressure similar to that of human body fluids.

[0063] This invention is achieved through the following technical solution:

[0064] This invention provides a hair growth composition with multiple synergistic effects, the composition comprising the following components in parts by weight: 7.5-10 parts by weight of sodium dodecyl sulfate, 8.4-16.1 parts by weight of plant extract, 0.01-0.1 parts by weight of copper peptide, and 0.5-1.5 parts by weight of coenzyme Q. 10 The plant extract comprises the following components by weight: 3-10 parts copidrome, 0.5-5 parts ammonium chloride, 1.5-3.5 parts adenosine, 2.2-3.3 parts nicotinamide, 0.3-1 parts sphingomyelin, 0.5-0.8 parts preservative, 27-38 parts permeabilizer, and 26-48.59 parts buffer solution; the plant extract further comprises the following components by weight: 3.2-5.3 parts camel thorn leaf extract, 3.6-6.4 parts macadamia nut oil, 0.3-1 parts selaginella extract, 0.3-1 parts black pepper extract, 0.5-1.1 parts green coffee bean extract, and 0.5-1.3 parts rosehip oil; the plant extract is subjected to nanofiltration separation.

[0065] In this invention, the preparation steps of the plant extract are as follows:

[0066] After crushing the plant, extractant A is added, followed by microwave treatment or pressing to obtain plant extract; the plant extract is then subjected to nanofiltration to obtain plant isolate; the plant isolate is concentrated by rotary evaporation or high-speed centrifugation, and then dried to obtain plant extract.

[0067] The nanofiltration separation process includes the following steps:

[0068] The plant extract was filtered through a 50-200 mesh metal filter, and then through a 5-micron polypropylene membrane to obtain filtrate 1. Filtrate 1 was then filtered through a 0.1-1 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2. Filtrate 2 was then filtered through a 0.01-0.05 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3. Filtrate 3 was then filtered through a 0.66 nanometer polyvinylidene fluoride membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is the plant isolate. The metal filter was selected from copper, silver, and stainless steel.

[0069] The plants mentioned include camel thorn leaves, macadamia nuts, selaginella, black pepper, green coffee beans, and rosehips; the extractant A is selected from one or more of ethanol, ether, ethyl acetate, propylene glycol, and butylene glycol. The selected extractant can extract the fat-soluble compounds from the above plants into the extract. The fat-soluble compounds are compounds that can be dissolved in ethanol, ether, ethyl acetate, propylene glycol, and butylene glycol.

[0070] The microwave treatment involves using 2300-2500MHz microwaves at 50-55 degrees Celsius for 15-25 minutes; microwave treatment significantly improves the extraction efficiency of the extractant from the broken plants. The pressing process uses a hydraulic press at 45-55 degrees Celsius for 0.5-1 hour; this pressing method is efficient, fast, and yields a stable product. The rotary evaporation concentration is achieved using a rotary evaporator at 45-55 degrees Celsius and -1 to -0.9 MPa for 5-8 hours; the high-speed centrifugal concentration is achieved using a centrifuge at 10000-13000 r / min for 1-1.5 hours; both rotary evaporation and centrifugal concentration can efficiently obtain stable concentrates. The drying process involves drying at 50-55 degrees Celsius for 72-80 hours; the plant extract obtained under these conditions is stable and does not become inactive or deformed.

[0071] Microwave extraction is used to more efficiently separate compounds from plant extracts into the extraction solvent, improving industrial efficiency. The resulting plant extract contains a large number of plant solid particles. The plant extract is then filtered through a 50-200 mesh metal filter and a 5-micron polypropylene membrane. This separation process removes broken plant solid particles. The filtrate is then filtered through a 0.1-1 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane. This separation process further removes solid particles larger than 0.1-1 microns. Filtrate 2 is then filtered through a 0.01-0.05 micrometer polyvinylidene fluoride (PVDF) membrane or an inorganic ceramic membrane. This separation process removes macromolecules larger than 0.01-0.05 micrometers, including crude fiber, crude protein, crude fat, and polysaccharides. Filtrate 3 is then filtered through a 0.66 nanometer PVDF membrane or an inorganic ceramic nanofiltration membrane. This separation process removes molecules larger than 0.66 nanometers, specifically molecules with a molecular weight of approximately 1000 Da or higher. After concentration and drying, the plant extract is obtained.

[0072] The microwave-membrane separation method described above separates crude fiber, crude protein, crude fat, and polysaccharides from camel thorn leaves. Molecules larger than 1000 Da are removed from the camel thorn leaf extract obtained by this method. The resulting extract contains flavonoids, alkaloids, polyphenols, tannins, amino acids, and minerals, while substances with molecular weights smaller than 500 Da easily penetrate the stratum corneum of the skin. These substances also possess anti-inflammatory and antioxidant properties. In contrast, camel thorn leaf extract obtained by conventional alcohol extraction contains high proportions of crude fiber, crude protein, crude fat, and polysaccharides. These components hinder skin penetration, preventing small molecules from passing through the stratum corneum. Compared to camel thorn leaf extract prepared by alcohol extraction, the microwave-membrane separation method significantly improves the skin penetration efficiency of the active ingredients, thereby enhancing the anti-inflammatory and antioxidant capabilities of the composition. The microwave-membrane separation method described above separates crude fiber, crude protein, crude fat, and polysaccharides from macadamia nuts. Molecules larger than 1000 Da are removed from the macadamia nut oil obtained by this method. The resulting macadamia nut oil contains flavonoids, tannins, polyphenols, oleic acid, and palmitoleic acid. This method significantly increases the probability of monounsaturated fatty acids entering the dermis, enhancing the ability to activate hair follicle stem cells. It also possesses anti-inflammatory and antioxidant properties, improving hair growth and development. Macadamia nut oil extracted using conventional alcohol extraction contains crude fiber, crude protein, crude fat, and polysaccharides in high proportions. However, these components hinder skin permeation, preventing small molecules from passing through the stratum corneum. The pressing process involves using a hydraulic press or screw press to press crushed macadamia nuts at 45-55 degrees Celsius to obtain the press liquor. The diluent is selected from one or more of ethanol, water, ethyl acetate, and diethyl ether.

[0073] The crude fiber, crude protein, crude fat, and polysaccharides in Selaginella tamariscina were separated by microwave-membrane separation. Molecules larger than 1000 Da were removed from the Selaginella tamariscina extract obtained by microwave-membrane separation. The Selaginella tamariscina extract prepared by this method contains paclitaxel flavonoids and dihydromyricetin, while substances with molecular weights smaller than 500 Da easily penetrate the stratum corneum of the skin. Simultaneously, paclitaxel flavonoids possess antioxidant, anti-inflammatory, melanin-inhibiting, soothing, and penetration-enhancing effects, while dihydromyricetin exhibits a novel and mild penetration-enhancing effect, combining anti-inflammatory and antioxidant properties, and is non-irritating, making it a penetration enhancer specifically for sensitive skin.

[0074] The above-described microwave-membrane separation method separates crude fiber, crude protein, crude fat, and polysaccharides from black pepper. Molecules larger than 1000 Da are removed from the black pepper extract obtained by this method. The black pepper extract prepared by this method contains piperine, while substances with molecular weights smaller than 500 Da easily penetrate the stratum corneum of the skin. Simultaneously, piperine has a skin-penetrating effect that promotes the transdermal absorption of polyphenols.

[0075] The microwave-membrane separation method described above separates crude fiber, crude protein, crude fat, and polysaccharides from green coffee beans. Molecules larger than 1000 Da are removed from the green coffee bean extract obtained by this method. The resulting green coffee bean extract contains chlorogenic acid, while substances with molecular weights smaller than 500 Da easily penetrate the stratum corneum of the skin. Furthermore, chlorogenic acid possesses potent anti-inflammatory and antioxidant effects.

[0076] The crude fiber, crude protein, crude fat, and polysaccharides in rosehips are separated by microwave-membrane separation. Molecules larger than 1000 Da are removed from the rosehip oil obtained by this method. The rosehip oil prepared by this method contains β-carotene, while substances with molecular weights smaller than 500 Da easily penetrate the stratum corneum of the skin. Simultaneously, β-carotene has the effects of activating hair follicle stem cells, promoting absorption, and directly protecting hair follicle cells.

[0077] Camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, and rosehip oil, extracted using conventional alcohol extraction methods, contain high proportions of crude fiber, crude protein, crude fat, and polysaccharides. These crude fibers, proteins, fats, and polysaccharides hinder skin permeability during the transdermal process, preventing small molecules from passing through the stratum corneum and reducing the efficiency of small molecule permeability, thus weakening the hair growth efficacy of the small molecule active ingredients in the composition. This indicates that nanofiltration controls the molecular weight of compounds in the plant extracts within the composition, enhancing the permeability of the active ingredients and thereby achieving a highly effective hair growth effect.

[0078] In this invention, sphingomyelin is added to form a nano-emulsion during the emulsification process. Nano-sized sphingomyelin can more easily penetrate the stratum corneum of the skin, and the sphingomyelin nano-emulsion can enhance the skin permeability of the active ingredients.

[0079] In this invention, the coenzyme Q... 10 Copinotone, Amenoxin, adenosine, and nicotinamide provide energy, increase the resilience of hair follicle blood vessels, improve metabolism, and protect nerves during hair growth and development, making the process more efficient.

[0080] In this invention, the preservative is one or more selected from phenoxyethanol, ethylhexylglycerin, and ethanol. Phenoxyethanol and ethylhexylglycerin are preferred preservatives. The penetrant is selected from one or more selected from ethanol, propylene glycol, butylene glycol, and PEG-40. Ethanol and propylene glycol are preferred penetrants, and ethanol and butylene glycol are also preferred penetrants. The buffer solution is a mixture of sodium citrate and citric acid; wherein the weight ratio of citric acid to sodium citrate in the buffer solution is 1.1-11.5:1.

[0081] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Unless otherwise specified, the reagents used in the embodiments can be obtained commercially. The specific embodiments are for further detailed explanation of this invention and are not intended to limit the scope of protection of this invention.

[0082] Example 1

[0083] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0084] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 26 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0085] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0086] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate, and 25.8 kg of water; the preparation steps of the plant extracts, including camel thorn leaf extract, selaginella extract, black pepper extract, and green coffee bean extract, are as follows:

[0087] (1) After crushing the plant, add ethanol and then treat it with microwave at 2300MHz at 50 degrees Celsius for 25 minutes to obtain plant extract;

[0088] (2) The plant extract was filtered through a 50-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0089] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0090] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0091] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0092] (6) The plant isolate was concentrated by high-speed centrifugation at 10000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract;

[0093] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0094] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 55 degrees Celsius for 0.5 hours to obtain plant extract;

[0095] (2) The plant extract was filtered through a 50-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0096] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0097] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0098] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0099] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0100] Example 2

[0101] Preparation of alcohol phase liquid: 5.3 kg of camel thorn leaf extract, 1 kg of selaginella extract, 1 kg of black pepper extract, 1.1 kg of green coffee bean extract, 1.3 kg of rosehip oil, 1.5 kg of copilot, and 1 kg of sphingomyelin were placed in 27 kg of permeabilizing agent and stirred at 57 degrees Celsius for 1 h to obtain alcohol phase liquid.

[0102] Preparation of aqueous phase liquid: 10 kg of sodium dodecyl sulfate, 5 kg of Amenos, 3.5 kg of adenosine, 3.3 kg of nicotinamide, and 0.8 kg of preservative were placed in 32.5 kg of buffer solution and stirred at 70 degrees Celsius for 1 h. Then 1.5 kg of cobilol was added and stirred for 0.8 h to obtain aqueous phase liquid.

[0103] Preparation of the hair growth and development composition: The 57°C alcohol phase liquid was injected into the 70°C aqueous phase liquid under stirring at 2000 r / min, emulsified at 7000 r / min for 10 min, then placed at 55°C and stirred at 2000 r / min for 1 h. Next, 3.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 Mix 0.1 kg of blue copper peptide and stir at 55 degrees Celsius for 1 hour to obtain a hair growth composition;

[0104] The penetrant contains 21 kg of ethanol and 6 kg of butylene glycol; the preservative contains 0.6 kg of phenoxyethanol and 0.2 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate, and 32.3 kg of water; the preparation steps of the plant extracts, including camel thorn leaf extract, selaginella extract, black pepper extract, and green coffee bean extract, are as follows:

[0105] (1) After crushing the plant, add propylene glycol and ethanol, and then treat it with microwave at 2500MHz at 55 degrees Celsius for 15 minutes to obtain plant extract;

[0106] (2) The plant extract was filtered through a 100-mesh copper filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0107] (3) Filter 1 through a 0.5-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0108] (4) Filter 2 through a 0.03-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0109] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0110] (6) The plant isolate was concentrated by high-speed centrifugation at 13000 r / min for 1 h, and then dried at 55 degrees Celsius for 72 h to obtain the plant extract;

[0111] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0112] (1) After crushing the plant, add propylene glycol and ethanol, and then press it with a hydraulic press at 45 degrees Celsius for 1 hour to obtain plant extract;

[0113] (2) The plant extract was filtered through a 90-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0114] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0115] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0116] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0117] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0118] Example 3

[0119] Preparation of the alcohol phase liquid: 3.3 kg of camel thorn leaf extract, 0.6 kg of selaginella extract, 0.6 kg of black pepper extract, 0.8 kg of green coffee bean extract, 0.9 kg of rosehip oil, 1.6 kg of copilot, and 0.6 kg of sphingomyelin were placed in 28.55 kg of permeabilizing agent and stirred at 50 degrees Celsius for 0.8 h to obtain the alcohol phase liquid;

[0120] Preparation of aqueous phase liquid: 8 kg of sodium dodecyl sulfate, 1.0 kg of Amenos, 2 kg of adenosine, 2.3 kg of nicotinamide, and 0.6 kg of preservative were placed in 43 kg of buffer solution and stirred at 65 degrees Celsius for 0.6 h. Then 1.6 kg of cobilol was added and stirred for 0.7 h to obtain aqueous phase liquid.

[0121] Preparation of the hair growth and development composition: The 50°C alcohol phase liquid was injected into the 65°C aqueous phase liquid under stirring at 1000 r / min, emulsified at 6500 r / min for 7 min, then placed at 50°C and stirred at 1000 r / min for 0.6 h. Next, 3.7 kg of macadamia nut oil and 0.8 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.05 kg of blue copper peptide at 50 degrees Celsius for 0.6 h.

[0122] The penetrant contains 22 kg of ethanol and 6.55 kg of PEG-40; the preservative contains 0.48 kg of phenoxyethanol and 0.12 kg of ethanol; the buffer contains 0.106 kg of citric acid, 0.094 kg of sodium citrate, and 42.8 kg of water; the plant extracts include camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps of the plant extracts are as follows:

[0123] (1) After crushing the plant, add ether and ethyl acetate, and then treat it with microwave at 2300MHz at 53 degrees Celsius for 20 minutes to obtain plant extract;

[0124] (2) The plant extract was filtered through a 50-mesh copper filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0125] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0126] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0127] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0128] (6) The plant isolate was concentrated by high-speed centrifugation at 12000 r / min for 1.3 h, and then dried at 53 degrees Celsius for 76 h to obtain the plant extract;

[0129] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0130] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 48 degrees Celsius for 0.6 hours to obtain plant extract;

[0131] (2) The plant extract was filtered through an 80-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0132] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0133] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0134] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0135] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0136] Example 4

[0137] Preparation of the alcohol phase liquid: 4.6 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.6 kg of green coffee bean extract, 0.7 kg of rosehip oil, 2.5 kg of copilot, and 0.7 kg of sphingomyelin were placed in 29.01 kg of permeabilizing agent and stirred at 50 degrees Celsius for 0.8 h to obtain the alcohol phase liquid;

[0138] Preparation of aqueous phase liquid: 9 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 1.5 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 40 kg of buffer solution and stirred at 65 degrees Celsius for 0.6 h. Then 2.4 kg of cobilol was added and stirred for 0.7 h to obtain aqueous phase liquid.

[0139] Preparation of the hair growth and development composition: The 50°C alcohol phase liquid was injected into the 65°C aqueous phase liquid under stirring at 1000 r / min, emulsified at 6500 r / min for 7 min, then placed at 50°C and stirred at 1000 r / min for 0.6 h. Next, 4.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.09 kg of blue copper peptide at 50 degrees Celsius for 0.6 h.

[0140] The penetrant contains 22 kg of ethanol and 7.01 kg of PEG-40; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethanol; the buffer contains 0.156 kg of citric acid, 0.044 kg of sodium citrate, and 39.8 kg of water; the plant extracts include camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps of the plant extracts are as follows:

[0141] (1) After crushing the plant, add butanediol and ethanol, and then treat it with microwave at 2400MHz at 50 degrees Celsius for 25 minutes to obtain plant extract;

[0142] (2) The plant extract was filtered through a 50-mesh silver filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0143] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0144] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0145] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0146] (6) The plant isolate was concentrated by rotary evaporation at 45°C and -0.9 MPa for 8 hours, and then dried at 52°C for 79 hours to obtain the plant extract;

[0147] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0148] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 52 degrees Celsius for 0.7 hours to obtain plant extract;

[0149] (2) The plant extract was filtered through a 100-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0150] (3) Filter 1 through a 0.6-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0151] (4) Filter 2 through a 0.03-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0152] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0153] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0154] Example 5

[0155] Preparation of the alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 1.5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 27 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain the alcohol phase liquid;

[0156] Preparation of aqueous phase liquid: 10 kg of sodium dodecyl sulfate, 0.5 kg of Amenes, 1.5 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 46.09 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 1.5 kg of cobilol was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0157] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 3.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.01 kg of blue copper peptide at 45 degrees Celsius for 0.5 h.

[0158] The solution contains 20 kg of ethanol and 7 kg of propylene glycol as the penetrant; 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin as the preservative; 0.106 kg of citric acid, 0.094 kg of sodium citrate, and 45.89 kg of water as the buffer; and plant extracts including camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps for the plant extracts are as follows:

[0159] (1) After crushing the plant, add ethanol and then treat it with microwave at 2500MHz at 52 degrees Celsius for 22 minutes to obtain plant extract;

[0160] (2) The plant extract was filtered through a 50-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0161] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0162] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0163] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0164] (6) The plant isolate was concentrated by rotary evaporation at 55 degrees Celsius and -1 MPa for 5 hours, and then dried at 50 degrees Celsius for 80 hours to obtain the plant extract;

[0165] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0166] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 50 degrees Celsius for 0.8 hours to obtain plant extract;

[0167] (2) The plant extract was filtered through a 50-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0168] (3) Filter 1 through a 0.5-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0169] (4) Filter 2 through a 0.02-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0170] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0171] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0172] Example 6

[0173] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 55 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0174] Preparation of aqueous phase liquid: 7.5 kg sodium dodecyl sulfate, 0.5 kg amnesic acid, 2.29 kg adenosine, 2.2 kg nicotinamide, and 0.5 kg preservative were placed in 26 kg buffer solution and stirred at 70 degrees Celsius for 0.5 h. Then 5 kg coliform was added and stirred for 0.6 h to obtain aqueous phase liquid.

[0175] Preparation of the hair growth and development composition: The 55°C alcohol phase liquid was injected into the 70°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 55 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0176] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate, and 25.8 kg of water; the preparation steps of the plant extracts, including camel thorn leaf extract, selaginella extract, black pepper extract, and green coffee bean extract, are as follows:

[0177] (1) After crushing the plant, add ethanol and then treat it with microwave at 2300MHz at 50 degrees Celsius for 25 minutes to obtain plant extract;

[0178] (2) The plant extract was filtered through a 200-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0179] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0180] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0181] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0182] (6) The plant isolate was concentrated by high-speed centrifugation at 10000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract;

[0183] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0184] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 55 degrees Celsius for 0.5 hours to obtain plant extract;

[0185] (2) The plant extract was filtered through a 200-mesh silver filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0186] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0187] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0188] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0189] (6) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min for 1.5 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0190] Example 7

[0191] Preparation of the alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 1.5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 27 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain the alcohol phase liquid;

[0192] Preparation of aqueous phase liquid: 7.5 kg sodium dodecyl sulfate, 0.5 kg amnesic acid, 1.5 kg adenosine, 2.2 kg nicotinamide, and 0.5 kg preservative were placed in 48.59 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then 1.5 kg coliform was added and stirred for 0.6 h to obtain aqueous phase liquid.

[0193] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 3.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.01 kg of blue copper peptide at 45 degrees Celsius for 0.5 h.

[0194] The solution contains 20 kg of ethanol and 7 kg of propylene glycol as the penetrant; 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin as the preservative; 0.11 kg of citric acid, 0.1 kg of sodium citrate, and 48.38 kg of water as the buffer; and plant extracts including camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps for the plant extracts are as follows:

[0195] (1) After crushing the plant, add ethanol and then treat it with microwave at 2500MHz at 52 degrees Celsius for 22 minutes to obtain plant extract;

[0196] (2) The plant extract was filtered through a 200-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0197] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0198] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0199] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0200] (6) The plant isolate was concentrated by rotary evaporation at 55 degrees Celsius and -0.95 MPa for 7 h, and then dried at 50 degrees Celsius for 80 h to obtain the plant extract;

[0201] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0202] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 50 degrees Celsius for 0.8 hours to obtain plant extract;

[0203] (2) The plant extract was filtered through a 200-mesh silver filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0204] (3) Filter 1 through a 0.5-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0205] (4) Filter 2 through a 0.02-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0206] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0207] (6) The plant isolate was concentrated by high-speed centrifugation at 13000 r / min for 1 h, and then dried at 55 degrees Celsius for 72 h to obtain the plant extract.

[0208] Example 8

[0209] Preparation of the alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 1.5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 27 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain the alcohol phase liquid;

[0210] Preparation of aqueous phase liquid: 7.5 kg sodium dodecyl sulfate, 0.5 kg amnesic acid, 1.5 kg adenosine, 2.2 kg nicotinamide, and 0.5 kg preservative were placed in 48.59 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then 1.5 kg coliform was added and stirred for 0.6 h to obtain aqueous phase liquid.

[0211] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 3.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.01 kg of blue copper peptide at 45 degrees Celsius for 0.5 h.

[0212] The penetrant contains 20 kg of ethanol and 7 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.106 kg of citric acid, 0.094 kg of sodium citrate, and 48.39 kg of water; the plant extracts include camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps of the plant extracts are as follows:

[0213] (1) After crushing the plant, add ethanol and then treat it with microwave at 2500MHz at 52 degrees Celsius for 22 minutes to obtain plant extract;

[0214] (2) The plant extract was filtered through a 100-mesh copper filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0215] (3) Filter 1 through a 0.1-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0216] (4) Filter the filtrate 2 through a 0.01-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0217] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0218] (6) The plant isolate was concentrated by rotary evaporation at 55 degrees Celsius and -0.9 MPa for 8 hours, and then dried at 50 degrees Celsius for 80 hours to obtain the plant extract;

[0219] The preparation steps for rosehip oil and macadamia nut oil from plant extracts are as follows:

[0220] (1) After crushing the plant, add ethanol and then press it with a hydraulic press at 50 degrees Celsius for 0.8 hours to obtain plant extract;

[0221] (2) The plant extract was filtered through a 50-mesh copper filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0222] (3) Filter 1 through a 0.5-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2;

[0223] (4) Filter 2 through a 0.02-micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3;

[0224] (5) Filtrate 3 is filtered again through a 0.66 nm polyvinylidene fluoride filter membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4, which is a plant isolate.

[0225] (6) The plant isolate was concentrated by high-speed centrifugation at 12000 r / min for 1.2 h, and then dried at 53 degrees Celsius for 76 h to obtain the plant extract.

[0226] Comparative Example 1

[0227] Preparation of alcohol phase liquid: 38 kg of penetrant was stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0228] Preparation of aqueous phase liquid: 0.5 kg of preservative was placed in 26 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h to obtain aqueous phase liquid;

[0229] Preparation of hair growth composition: Under stirring at 800 r / min, the alcohol phase liquid at 45 degrees Celsius was injected into the aqueous phase liquid at 60 degrees Celsius, emulsified at 6000 r / min for 5 min, and then placed at 45 degrees Celsius and stirred at 800 r / min for 0.5 h to obtain the hair growth composition;

[0230] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 25.8 kg of water.

[0231] Comparative Example 2

[0232] Preparation of alcohol phase liquid: 5 kg of cobalt was placed in 38 kg of penetrant and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0233] Preparation of aqueous phase liquid: 2.5 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 42.51 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0234] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 1.5 kg of coenzyme Q was added. 10 The hair growth composition was obtained by stirring at 45 degrees Celsius for 0.5 hours.

[0235] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 42.31 kg of water.

[0236] Comparative Example 3

[0237] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0238] Preparation of aqueous phase liquid: 2.5 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 31 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0239] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0240] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 30.8 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of selaginella extract is the same as in Example 1; the preparation method of black pepper extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1; the preparation method of macadamia nut oil is the same as in Example 1.

[0241] Comparative Example 4

[0242] Preparation of alcohol phase liquid: 5 kg of cobalt was placed in 38 kg of penetrant and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0243] Preparation of aqueous phase liquid: 7.5 kg sodium dodecyl sulfate, 0.5 kg amnesic acid, 2.29 kg adenosine, 2.2 kg nicotinamide, and 0.5 kg preservative were placed in 37.51 kg buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then 5 kg coliform was added and stirred for 0.6 h to obtain aqueous phase liquid.

[0244] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 1.5 kg of coenzyme Q was added. 10 The hair growth composition was obtained by stirring at 45 degrees Celsius for 0.5 hours.

[0245] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.171 kg of citric acid, 0.029 kg of sodium citrate and 37.31 kg of water.

[0246] Comparative Example 5

[0247] Preparation of alcohol phase liquid: 0.3 kg of black pepper extract and 5 kg of cobalt were placed in 38 kg of permeabilizer and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0248] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 37.2 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of cobilol was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0249] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 1.5 kg of coenzyme Q was added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0250] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.144 kg of citric acid, 0.056 kg of sodium citrate and 37 kg of water; the preparation method of black pepper extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1.

[0251] Comparative Example 6

[0252] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid.

[0253] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 32.9 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0254] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 1.5 kg of coenzyme Q was added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0255] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 32.7 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of selaginella extract is the same as in Example 1; the preparation method of black pepper extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1.

[0256] Comparative Example 7

[0257] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid.

[0258] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 26.61 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0259] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring at 45 degrees Celsius for 0.5 hours.

[0260] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.18 kg of citric acid, 0.02 kg of sodium citrate and 26.41 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1; the preparation method of macadamia nut oil is the same as in Example 1.

[0261] Comparative Example 8

[0262] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0263] Preparation of aqueous phase liquid: 7.3 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 27 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of cobilol was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0264] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 5.6 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0265] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.181 kg of citric acid, 0.019 kg of sodium citrate and 26.8 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of selaginella extract is the same as in Example 1; the preparation method of black pepper extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1; the preparation method of macadamia nut oil is the same as in Example 1.

[0266] Comparative Example 9

[0267] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0268] Preparation of aqueous phase liquid: 10.2 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.69 kg of adenosine, 2.6 kg of nicotinamide, and 0.5 kg of preservative were placed in 22.5 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0269] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0270] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 22.3 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of selaginella extract is the same as in Example 1; the preparation method of black pepper extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1; the preparation method of macadamia nut oil is the same as in Example 1.

[0271] Comparative Example 10

[0272] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, 5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 38 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0273] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 26 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0274] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0275] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate, and 25.8 kg of water; the plant extracts include camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps of the plant extracts are as follows:

[0276] (1) After crushing the plant, add ethanol and then treat it with microwave at 2300MHz at 50 degrees Celsius for 25 minutes to obtain plant extract;

[0277] (2) The plant extract was filtered through a 50-mesh stainless steel filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1.

[0278] (3) Filtrate 1 is filtered again through a 0.1-micron polyvinylidene fluoride filter membrane or an inorganic ceramic filter membrane to obtain filtrate 2, which is a plant isolate;

[0279] (4) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min and then dried at 50 degrees Celsius for 80 h to obtain the plant extract.

[0280] Comparative Example 11

[0281] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 0.5 kg of rosehip oil, and 5 kg of copilot were placed in 38 kg of permeating agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid.

[0282] Preparation of aqueous phase liquid: 7.5 kg of sodium dodecyl sulfate, 0.5 kg of amnesic acid, 2.29 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 26.3 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 5 kg of coliform was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0283] Preparation of the hair growth and development composition: Under stirring at 800 r / min, the 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Next, 6.4 kg of macadamia nut oil and 1.5 kg of coenzyme Q were added. 10 Mix 0.01 kg of blue copper peptide and stir at 45 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0284] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 26.1 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 1; the preparation method of selaginella extract is the same as in Example 1; the preparation method of black pepper extract is the same as in Example 1; the preparation method of green coffee bean extract is the same as in Example 1; the preparation method of rosehip oil is the same as in Example 1; the preparation method of macadamia nut oil is the same as in Example 1.

[0285] Comparative Example 12

[0286] Preparation of alcohol phase liquid: 5.3 kg of camel thorn leaf extract, 1 kg of selaginella extract, 1 kg of black pepper extract, 1.1 kg of green coffee bean extract, 1.3 kg of rosehip oil, and 1.5 kg of copilot were placed in 28 kg of permeating agent and stirred at 57 degrees Celsius for 1 h to obtain alcohol phase liquid.

[0287] Preparation of aqueous phase liquid: 10 kg of sodium dodecyl sulfate, 5 kg of Amenos, 3.5 kg of adenosine, 3.3 kg of nicotinamide, and 0.8 kg of preservative were placed in 32.5 kg of buffer solution and stirred at 70 degrees Celsius for 1 h. Then 1.5 kg of cobilol was added and stirred for 0.8 h to obtain aqueous phase liquid.

[0288] Preparation of the hair growth and development composition: The 57°C alcohol phase liquid was injected into the 70°C aqueous phase liquid under stirring at 2000 r / min, emulsified at 7000 r / min for 10 min, then placed at 55°C and stirred at 2000 r / min for 1 h. Next, 3.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 Mix 0.1 kg of blue copper peptide and stir at 55 degrees Celsius for 1 hour to obtain a hair growth composition;

[0289] The penetrant contained 21 kg of ethanol and 7 kg of butylene glycol; the preservative contained 0.6 kg of phenoxyethanol and 0.2 kg of ethylhexylglycerin; the buffer contained 0.184 kg of citric acid, 0.016 kg of sodium citrate and 32.3 kg of water; the preparation method of camel thorn leaf extract was the same as in Example 2; the preparation method of selaginella extract was the same as in Example 2; the preparation method of black pepper extract was the same as in Example 2; the preparation method of green coffee bean extract was the same as in Example 2; the preparation method of rosehip oil was the same as in Example 2; the preparation method of macadamia nut oil was the same as in Example 2.

[0290] Comparative Example 13

[0291] Preparation of the alcohol phase liquid: 3.3 kg of camel thorn leaf extract, 0.6 kg of selaginella extract, 0.6 kg of black pepper extract, 0.8 kg of green coffee bean extract, 0.9 kg of rosehip oil, 1.6 kg of copilot, and 0.6 kg of sphingomyelin were placed in 28.55 kg of permeabilizing agent and stirred at 50 degrees Celsius for 0.8 h to obtain the alcohol phase liquid;

[0292] Preparation of aqueous phase liquid: 8 kg of sodium dodecyl sulfate, 1.0 kg of Amenos, 2 kg of adenosine, 2.3 kg of nicotinamide, and 0.6 kg of preservative were placed in 43 kg of buffer solution and stirred at 65 degrees Celsius for 0.6 h. Then 1.6 kg of cobilol was added and stirred for 0.7 h to obtain aqueous phase liquid.

[0293] Preparation of the hair growth and development composition: The 50°C alcohol phase liquid was injected into the 65°C aqueous phase liquid under stirring at 1000 r / min, emulsified at 6500 r / min for 7 min, then placed at 50°C and stirred at 1000 r / min for 0.6 h. Next, 3.7 kg of macadamia nut oil and 0.8 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring 0.05 kg of blue copper peptide at 50 degrees Celsius for 0.6 h.

[0294] The penetrant contains 22 kg of ethanol and 6.55 kg of PEG-40; the preservative contains 0.48 kg of phenoxyethanol and 0.12 kg of ethanol; the buffer contains 0.106 kg of citric acid, 0.094 kg of sodium citrate, and 42.8 kg of water; the plant extracts include camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, and macadamia nut oil. The preparation steps of the plant extracts are as follows:

[0295] (1) After crushing the plant, add ethanol and then treat it with microwave at 2300MHz at 50 degrees Celsius for 25 minutes to obtain plant extract;

[0296] (2) The plant extract was filtered through a 100-mesh copper filter and then through a 5-micron polypropylene filter membrane to obtain filtrate 1;

[0297] (3) Filtrate 1 is filtered again through a 0.1-micron polyvinylidene fluoride filter membrane or an inorganic ceramic filter membrane to obtain filtrate 2, which is a plant isolate;

[0298] (4) The plant isolate was concentrated by high-speed centrifugation at 10,000 r / min and then dried at 55 degrees Celsius for 72 h to obtain the plant extract.

[0299] Comparative Example 14

[0300] Preparation of alcohol phase liquid: 4.6 kg of camel thorn leaf extract, 0.6 kg of green coffee bean extract, 0.7 kg of rosehip oil, 2.5 kg of copilot and 0.7 kg of sphingomyelin were placed in 29.7 kg of permeabilizing agent and stirred at 50 degrees Celsius for 0.8 h to obtain alcohol phase liquid;

[0301] Preparation of aqueous phase liquid: 9 kg of sodium dodecyl sulfate, 0.5 kg of Amenos, 1.5 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 40 kg of buffer solution and stirred at 65 degrees Celsius for 0.6 h. Then 2.4 kg of cobilol was added and stirred for 0.7 h to obtain aqueous phase liquid.

[0302] Preparation of the hair growth and development composition: The 50°C alcohol phase liquid was injected into the 65°C aqueous phase liquid under stirring at 1000 r / min, emulsified at 6500 r / min for 7 min, then placed at 50°C and stirred at 1000 r / min for 0.6 h. Next, 4.6 kg of macadamia nut oil and 0.5 kg of coenzyme Q were added. 10 The hair growth composition was obtained by stirring at 50 degrees Celsius for 0.6 hours.

[0303] The penetrant contained 22 kg of ethanol and 7.7 kg of PEG-40; the preservative contained 0.4 kg of phenoxyethanol and 0.1 kg of ethanol; the buffer contained 0.156 kg of citric acid, 0.044 kg of sodium citrate and 39.8 kg of water; the preparation method of camel thorn leaf extract was the same as in Example 4; the preparation method of green coffee bean extract was the same as in Example 4; the preparation method of rosehip oil was the same as in Example 4; the preparation method of macadamia nut oil was the same as in Example 4.

[0304] Comparative Example 15

[0305] Preparation of alcohol phase liquid: 3.2 kg of camel thorn leaf extract, 0.3 kg of selaginella extract, 0.3 kg of black pepper extract, 0.5 kg of green coffee bean extract, 1.5 kg of copilot, and 0.3 kg of sphingomyelin were placed in 31.1 kg of permeabilizing agent and stirred at 45 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0306] Preparation of aqueous phase liquid: 10 kg of sodium dodecyl sulfate, 0.5 kg of Amenes, 1.5 kg of adenosine, 2.2 kg of nicotinamide, and 0.5 kg of preservative were placed in 46.09 kg of buffer solution and stirred at 60 degrees Celsius for 0.5 h. Then, 1.5 kg of cobilol was added and stirred for 0.6 h to obtain the aqueous phase liquid.

[0307] Preparation of the hair growth and development composition: The 45°C alcohol phase liquid was injected into the 60°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 0.5 kg of coenzyme Q was added. 10 The hair growth composition was obtained by stirring 0.01 kg of blue copper peptide at 45 degrees Celsius for 0.5 h.

[0308] The penetrant contains 23 kg of ethanol and 8.1 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.106 kg of citric acid, 0.094 kg of sodium citrate and 45.89 kg of water; the preparation method of camel thorn leaf extract is the same as in Example 5; the preparation method of selaginella extract is the same as in Example 5; the preparation method of black pepper extract is the same as in Example 5; the preparation method of green coffee bean extract is the same as in Example 5.

[0309] Comparative Example 16

[0310] Preparation of alcohol phase liquid: 0.3 kg of black pepper extract, 0.5 kg of rosehip oil, and 5 kg of cobiol were placed in 38 kg of permeabilizing agent and stirred at 55 degrees Celsius for 0.5 h to obtain alcohol phase liquid;

[0311] Preparation of aqueous phase liquid: 7.5 kg sodium dodecyl sulfate, 0.5 kg amnesic acid, 2.29 kg adenosine, 2.2 kg nicotinamide, and 0.5 kg preservative were placed in 36.7 kg buffer solution and stirred at 70 degrees Celsius for 0.5 h. Then 5 kg coliform was added and stirred for 0.6 h to obtain aqueous phase liquid.

[0312] Preparation of the hair growth and development composition: The 55°C alcohol phase liquid was injected into the 70°C aqueous phase liquid under stirring at 800 r / min, emulsified at 6000 r / min for 5 min, then placed at 45°C and stirred at 800 r / min for 0.5 h. Following this, 1.5 kg of coenzyme Q was added. 10 Mix 0.01 kg of blue copper peptide and stir at 55 degrees Celsius for 0.5 h to obtain a hair growth composition;

[0313] The penetrant contains 30 kg of ethanol and 8 kg of propylene glycol; the preservative contains 0.4 kg of phenoxyethanol and 0.1 kg of ethylhexylglycerin; the buffer contains 0.184 kg of citric acid, 0.016 kg of sodium citrate and 36.5 kg of water; the preparation method of black pepper extract is the same as in Example 6; the preparation method of rosehip oil is the same as in Example 6.

[0314] Animal experiments were conducted on samples from the embodiments of this application for anti-hair loss cosmetics:

[0315] (a) Transdermal absorption (permeation enhancement) test:

[0316] 1. Test substance

[0317] Samples of Examples 1-4, Sample 7 of Comparative Examples, and Samples 10-14 of Comparative Examples.

[0318] 2. Test Methods

[0319] Thirty SPF-grade, 6-8 week old, half male and half female C57BL / 6 mice were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd., Animal Production License: SCXK (Lu) 2022 0006, Animal Use License: SYXK (Lu) 2023 0032. The rearing environment was maintained at (23±2)℃ and (50±5)% humidity, with free access to food and water.

[0320] The effects of transdermal absorption (permeation enhancement) assays on the transdermal absorption (permeation enhancement) capabilities of Examples 1-4, Comparative Examples 7, and Comparative Examples 10-14 on the antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel biflavonoids, and chlorogenic acid) were evaluated. The specific methods are as follows:

[0321] In vitro transdermal experiments were conducted using a vertical diffusion cell with nude mouse skin as the model (abdominal skin, with subcutaneous fat and blood vessels removed). The receiving solution was PBS. The skin patch was fixed between the supply and receiving cells, with the skin layer facing down, and equilibrated for 20 min. Samples were collected and added to the supply cell, and the receiving solution was collected after 2 h, 6 h, 12 h, and 24 h. Methanol was added to the collected receiving solution, and the solution was ultrasonically treated for 30 min at a ratio of sample:methanol = 1:9 (v / v). After filtration through a 0.45 μm organic filter membrane, the contents of antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel biflavonoids, and chlorogenic acid) were determined by high performance liquid chromatography (HPLC, Shimadzu, Japan), and the cumulative permeation per unit area was calculated accordingly. Each group of experiments was conducted in triplicate, and the arithmetic mean of the experimental results was taken. The formula for calculating the cumulative permeation per unit area on the skin patch is shown in formula (1):

[0322] (Formula 1)

[0323] Among them, Q n The cumulative transmittance per unit area (ng / cm²) of the antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel biflavonoids, chlorogenic acid) in the sample at time t. 2 ), A is the permeation area, C n C represents the concentration of antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel biflavonoids, and chlorogenic acid) in the sample at time t. i V represents the concentration of antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel flavonoids, chlorogenic acid) in the sample before time t, and V is the total volume of the receiving liquid. i Let be the sampling volume for the i-th time.

[0324] 3. Test Results

[0325] Table 1. Permeability of antioxidant and anti-inflammatory active ingredients (flavonoids, paclitaxel flavonoids, chlorogenic acid) in samples from Examples 1-4, Comparative Examples 7, and Comparative Examples 10-14

[0326]

[0327] The transdermal absorption characterization results of samples from Examples 1-4, Comparative Examples 7, and Comparative Examples 10-14 are shown in Table 1. The cumulative permeation per unit area of ​​the antioxidant and anti-inflammatory active ingredients in samples from Examples 1-4 at 2h, 6h, 12h, and 24h was significantly greater than that of Comparative Examples 7 and 10-14. The difference between Comparative Examples 7 and 14 and Samples from Examples 1-4 is the absence of three components that promote skin penetration: copper peptide, Selaginella tamariscina extract, and black pepper extract. The difference between Comparative Examples 10 and 13 and Samples from Examples 1-4 is that the added plant extract components were not subjected to nanofiltration membrane separation. The difference between Comparative Examples 11 and 12 and Samples from Examples 1-4 is the absence of sphingomyelin, which can be emulsified to form nanoemulsions. This indicates that in this invention, nanofiltration membrane separation, components that promote skin penetration, and nanoemulsions formed by sphingomyelin emulsification have a synergistic effect in enhancing the transdermal permeability of the antioxidant and anti-inflammatory active ingredients.

[0328] (ii) Security Testing

[0329] 1. Test substance

[0330] Samples of Examples 1, 5, and 6; samples of Comparative Examples 2-6; samples of Comparative Examples 8-9; and samples of Comparative Examples 15-16.

[0331] 2. Test Methods

[0332] After 7 days of acclimatization feeding, 120 mice were randomly divided into 12 groups of 10 mice each, with 5 males and 5 females. Samples from Examples 1, 5, and 6, samples from Comparative Examples 2-6, samples from Comparative Examples 8-9, and samples from Comparative Examples 15-16 were then applied to the backs of the mice in the 12 groups once daily for 14 consecutive days.

[0333] Administration and Observation: 24 hours before the experiment, the hair on both sides of the spine of the mice was shaved, covering an area of ​​2cm × 3cm. 0.2ml of the test substance was applied directly to the mouse skin, covering an area of ​​2cm × 2.5cm. The untreated area served as a control. The drug was administered once daily for 14 consecutive days. Starting from the second day, the hair was shaved before each application, and the test substance was cleaned with water. After the experiment, the test substance was removed with warm water, and the skin condition of the mice was observed. Results were observed one hour later.

[0334] According to the skin irritation / corrosion test table 2 of the "Cosmetic Safety Technical Specifications" (2015 edition), the control area and the test area were treated in the same way.

[0335] Results evaluation: The average score per mouse per day was calculated according to the following formula (2), and the skin irritation intensity of the mice was determined according to Table 3 of the Skin Irritation / Corrosion Test in the "Cosmetic Safety Technical Specifications" (2015 edition).

[0336] During the experiment, observe whether the skin has any symptoms other than skin irritation.

[0337] Number of test animals 14 (Formula 2)

[0338] Table 2 Skin Irritation Response Scores

[0339]

[0340] Table 3 Skin Irritation Intensity Grading

[0341]

[0342] 3. Test Results

[0343] Table 4. Skin irritation test results of samples from Examples 1, 5, and 6, Comparative Examples 2-6, Comparative Examples 8-9, and Comparative Examples 15-16.

[0344]

[0345] The skin irritation test results for samples from Examples 1, 5, and 6, Comparative Examples 2-6, Comparative Examples 8-9, and Comparative Examples 15-16 are shown in Table 4. Samples from Examples 1, 5, and 6 were non-irritating in the skin irritation test. Samples from Comparative Examples 2, 3, 6, 8, and 15 were non-irritating in the skin irritation test. Sample from Comparative Example 9 was moderately irritating in the skin irritation test, indicating that the composition of this invention contains 10.2% sodium lauryl sulfate, exceeding the repair limit of the composition's anti-inflammatory and antioxidant effects, thus disrupting the homeostasis of hair follicles. Samples from Examples 1, 5, and 6, and Comparative Examples 2, 3, and 8 were non-irritating in the skin irritation test. The sodium lauryl sulfate content in Samples 1, 5, and 6 was 7.5%, 10%, and 7.5%, respectively, while the sodium lauryl sulfate content in Comparative Examples 2, 3, and 8 was 2.5%, 2.5%, and 7.3%, respectively. This indicates that in the present invention, in the absence of excellent anti-inflammatory and repairing components, 7.5-10% sodium lauryl sulfate can damage the skin, while the composition containing 10.2% sodium lauryl sulfate exceeds the limit of the repair effect of the composition, and the composition containing 7.3% sodium lauryl sulfate does not cause skin damage. Comparative Example 4 was moderately irritating in the skin irritation test, indicating that the 7.5% sodium lauryl sulfate in the present invention can damage the skin and cause inflammation. Comparative Examples 5 and 16 were moderately irritating in the skin irritation test. The difference between Comparative Examples 5 and 16 and Examples 1 and 6 is that they did not contain camel thorn leaf extract, macadamia nut oil, selaginella extract, green coffee bean extract, or sphingomyelin; camel thorn leaf extract, sphingomyelin, selaginella extract, green coffee bean extract, and macadamia nut oil contain excellent anti-inflammatory components. When the composition lacks excellent anti-inflammatory and antioxidant components, it can cause some discomfort. This indicates that in this invention, by simultaneously performing micro-damage and repair, that is, by simultaneously activating hair follicle stem cells through micro-damage and carrying out anti-inflammatory repair, hair follicle homeostasis can be maintained while achieving efficient hair growth.

[0346] (iii) Validity test

[0347] 1. Test substance

[0348] Example 1: Sample and positive control drug were minoxidil and physiological saline.

[0349] 2. Main Instruments

[0350] Embedding machine (YD-6L); Paraffin microtome (Leica HistoCore BIOCUT); Inverted microscope (Nikon Ts2R); Electrophoresis apparatus (BIO-RAD mini protean 3 cell); Electroporation apparatus (HOEFER, model TE77XP); Microplate reader (Raber MK3 microplate reader from Finland).

[0351] 3. Animal grouping and treatment

[0352] Sixteen mice were acclimatized for 7 days and then randomly divided into four groups of four (two males and two females) each. Except for the normal control group, the remaining mice were subcutaneously injected with testosterone propionate solution (5 mg / kg / day) for three consecutive weeks. Dosage: The hair removal area for each mouse was approximately 2cm × 3cm. The dosage was 0.1 mL per mouse, ensuring even coverage of the hair removal area. Administration was twice daily for three consecutive weeks.

[0353] Group 1 (n=4): Normal feeding.

[0354] The second model control group (n=4): physiological saline was applied to the back twice a day for three consecutive weeks.

[0355] The third group (n=4) of minoxidil positive drug model groups: After hair removal and administration of testosterone solution, 5% minoxidil tincture solution (0.1 mL) was applied to the back twice daily for three consecutive weeks.

[0356] Group 4, Sample Group 1 (n=4): After hair removal, testosterone solution was applied to the back of the sample 1 (0.1 mL) twice daily for three consecutive weeks.

[0357] 4. Data Collection

[0358] We took photos daily to record the hair growth on the backs of the mice; we used HE staining to detect the pathological expression of mouse skin tissue, focusing on the miniaturization index of hair follicles.

[0359] Miniaturized hair follicle ratio: count the number and ratio of terminal hairs (coarse) and vellus hairs (fine), measure the diameter of the thickest part of the hair follicle, dermal thickness, etc.

[0360] Western blot analysis was used to detect the expression of 5α-reductase (SRD5A2) in mouse skin tissue.

[0361] 5. Statistical Analysis

[0362] The results were analyzed using GraphPad Prism 8 software, and the data are expressed as (x±s). The T-test was used to compare the two groups of data, and the p-value < 0.05 was considered statistically significant.

[0363] 6. Results Analysis

[0364] Table 5. Number of terminal hairs, number of vellus hairs, and ratio of terminal hairs to vellus hairs in local areas of mice in the control group, model group, positive group, and sample group of Example 1.

[0365]

[0366]

[0367] Table 6. Dermal thickness of mice in control group, model group, positive group, and sample group of Example 1.

[0368]

[0369] Table 7. Terminal hair diameter of mice in the control group, model group, positive group, and sample group of Example 1.

[0370]

[0371]

[0372] Table 8. Vellus diameter of mice in control group, model group, positive group, and sample group of Example 1.

[0373]

[0374] Table 9. Western blot analysis of 5α-reductase (SRD5A2) expression in mouse skin tissue of mice in the control group, model group, positive group, and sample group of Example 1.

[0375]

[0376]

[0377] Note: **P<0.01 compared to the model group, *P<0.05 compared to the model group

[0378] In Tables 5, 6, 7, 8, and 9, the control group is the normal control group, the model group is the model control group, the positive group is the minoxidil positive drug administration model group, and the sample group of Example 1 is the sample group of Example 1. Table 5 is... Figure 2 and Figure 3 The original data, Figure 2 and Figure 3 The values ​​in the table are the average values ​​of each group in Table 5. Table 6 shows the dermal thickness of mice in the control group, model group, positive group, and sample group of Example 1. Table 7 shows the terminal hair diameter of mice in the control group, model group, positive group, and sample group of Example 1. Table 8 shows the vellus hair diameter of mice in the control group, model group, positive group, and sample group of Example 1. Table 9 shows... Figure 1 The original data, Figure 1 The value in the table is the average SRD5A2 expression level for each group in Table 9. Figure 11 and Figure 12 This example illustrates the expression of 5α-reductase (SRD5A2) in mouse skin tissue from the control group, model group, positive group, and sample from Example 1 in animal experiments. Figure 11 and Figure 12 The original spectral data in Table 9 are based on... Figure 11 and Figure 12 The expression of 5α-reductase (SRD5A2) in the skin tissue of medullomyeloid mice was obtained. Among them, Figure 11 and Figure 12 The control group was the normal control group, the model group was the model control group, the positive group was the minoxidil positive drug administration model group, and the sample group of Example 1 was the sample group of Example 1. Figure 16 and Figure 17 HE staining was performed on day 21 of the control group, model group, positive group, and samples from Example 1 in the animal experiments of this embodiment to detect the pathological expression and hair follicle status of mouse skin tissue. Figure 16 and Figure 17 The original spectral data in Table 5-8 are as follows. The data in Table 5-8 are based on... Figure 16 and Figure 17 HE staining was used to detect the pathological expression and hair follicle status of mouse skin tissue.

[0379] Figure 1 , Figure 2 , Figure 3 and Figure 4 The control group was the normal control group, the model group was the model control group, the positive group was the minoxidil positive drug administration model group, and Example 1 was the Example 1 sample group. Observation Figure 1 , Figure 2 , Figure 3 and Figure 4 The study found that, compared with the normal control group, the number of terminal hairs in the model control group was significantly reduced (from 29.67±5.30 to 10.22±3.80, P<0.05); the number of vellus hairs was significantly increased (from 1.92±1.56 to 8.00±3.16); and the ratio of terminal hairs to vellus hairs decreased significantly (from 18.26±11.58 to 1.61±1.32); there were no significant differences in the diameter of the thickest part of the hair follicle and the thickness of the dermis. These results indicate that the androgen-induced male pattern baldness model mice exhibited typical pathological features of follicle miniaturization and the transformation of terminal hairs into vellus hairs, confirming the successful establishment of the model. Compared with the model control group, the minoxidil positive drug model group showed a significant increase in the number of terminal hairs (32.82±19.44, P<0.01), a decrease in the number of vellus hairs, a recovery in the ratio of terminal hairs to vellus hairs (12.89±10.26), and improvements in hair follicle diameter and dermal thickness, indicating that the positive drug can effectively reverse the hair miniaturization process in the model mice.

[0380] Model construction successful: Compared with the normal control group, the expression level of 5α-reductase (SRD5A2) in the skin tissue of mice in the model control group was significantly upregulated, and typical pathological features of hair follicle miniaturization, such as reduced terminal hair number, increased vellus hair number, and decreased terminal hair / vellus hair ratio, were observed.

[0381] Example 1 sample can significantly inhibit SRD5A2 expression: Compared with the model control group, the expression level of SRD5A2 in the Example 1 sample group was significantly reduced, and the inhibition effect was obvious. This indicates that the tested sample Example 1 can reduce the production of dihydrotestosterone (DHT) by downregulating SRD5A2 expression, thereby alleviating the damage of androgens to hair follicles.

[0382] Example 1 samples effectively reversed the hair follicle miniaturization process: Compared with the model control group, the number of terminal hairs in the Example 1 sample group was significantly increased (P<0.05 or P<0.01), the number of vellus hairs decreased, and the ratio of terminal hairs to vellus hairs rebounded, indicating that the tested samples can promote the conversion of vellus hairs into terminal hairs and improve hair structure. Example 1 samples exhibited certain anti-andruff activity, and its mechanism of action may be related to inhibiting SRD5A2 expression, reducing DHT production, and reversing hair follicle miniaturization.

[0383] observe Figure 5 The results showed that the anti-hair loss effect of the sample from Example 1 was close to that of the positive group, indicating that our Example 1 has a significant anti-hair loss effect. Figure 5 The control group consisted of a normal control group, the model group consisted of a model control group, the positive group consisted of a minoxidil positive drug administration model group, and Example 1 consisted of the Example 1 sample group. Figure 10 The growth of hair on the back of mice in the sample group of Example 1 was recorded by photographing from day 1 to day 21 in the animal experiment of Example 1.

[0384] The samples from the embodiments of this application were subjected to human trial testing as anti-hair loss cosmetics:

[0385] (a) Security Testing

[0386] 1. Materials and Methods

[0387] 1.1. Test material: Sample of Example 1.

[0388] 1.2. Subjects: A total of 30 subjects, 18 males and 12 females, aged 31 to 57 years, with a mean age of 41.8 ± 7.91 years, meeting the subject voluntary inclusion criteria.

[0389] 1.3. Trial Method: Subjects used the test product directly according to the usage characteristics and methods indicated on the cosmetic product label, i.e., once a day, using 1ml each time. After applying the test product, gently massage with the fingertips for at least 15 minutes, and then leave it on for at least two hours. Subjects' skin reactions were followed up by telephone once a week, and the results were recorded according to the grading standards for adverse skin reactions in human trials in the "Cosmetic Safety Technical Specifications" (2015 edition) (as shown in Table 10). The trial period was 12 weeks.

[0390] Table 10 Grading Standards for Skin Reactions in Human Trial Use

[0391]

[0392] 2. Test Results

[0393] Table 11 Results of human trials for anti-hair loss cosmetics

[0394]

[0395] Note: The skin reactions of the 30 subjects at different observation times are shown in Table 12.

[0396] Human trials showed that 0 out of 30 participants experienced adverse skin reactions. This indicates that the composition of this invention is safe and non-irritating.

[0397] Table 12 Skin reactions of 30 subjects at different observation times.

[0398]

[0399]

[0400] (ii) Validity test

[0401] 1. Test substance

[0402] Samples from Examples 1-3, minoxidil, samples from Comparative Examples 2-3, samples from Comparative Examples 6-8, and samples from Comparative Examples 10-11

[0403] 2. Subjects

[0404] 330 volunteers with various types of hair loss (average daily hair loss of 180-210 strands, localized hair loss of 18-21 strands) were randomly selected, including 200 males and 130 females aged 30-55 years. They were randomly divided into 11 groups of 30 each. Each group received care using samples from Examples 1-3, minoxidil, comparative samples 2-3, comparative samples 6-8, and comparative samples 10-11, respectively. No other care products were used during the period. In addition to the main conditions mentioned above, the subjects were generally required to have no other diseases that could affect the results of this observation, not to have used similar hair growth products within the past month, and to be able to voluntarily cooperate in completing the observation as required.

[0405] 3. Test method

[0406] The method of use is as follows: Subjects should use the test product directly according to the usage characteristics and methods indicated on the cosmetic product label, i.e., once a day, using 1ml each time. After applying the test product, gently massage with the fingertips for at least 15 minutes, and then leave it on for at least two hours. Continue use for 12 weeks, observing hair growth and average daily hair loss in each group of volunteers during the 12th week.

[0407] 4. Evaluation of therapeutic effect

[0408] Effect criteria: (1) Significant effect: hair loss stops and black hair grows back in the hair loss area; (2) Effective: hair loss is significantly improved and black hair grows back in some hair loss areas; (3) Slight effect: hair loss is improved and vellus hair grows in the hair loss area; (4) Ineffective: hair loss is not improved or reduced and no new hair grows in the hair loss area.

[0409] Hair loss statistics: Before and 12 weeks after use, volunteers' hair was combed by trained staff using the 60-comb method during interviews. The number of hairs lost was counted and recorded. The number of hairs lost after 12 weeks of use was statistically analyzed to evaluate the hair loss prevention effect of the serum. The statistical results were averaged.

[0410] 5. Test Results:

[0411] Table 13 Results of hair growth efficacy tests for samples from Examples 1-3, minoxidil, comparative examples 2-3, comparative examples 6-8, and comparative examples 10-11

[0412]

[0413] Table 14 Results of hair growth efficacy tests after 12 weeks for samples from Examples 1-3, minoxidil, Comparative Examples 2-3, Comparative Examples 6-8, and Comparative Examples 10-11.

[0414]

[0415] The results of the regrowth and hair growth efficacy tests for samples from Examples 1-3, minoxidil, comparative examples 2-3, comparative examples 6-8, and comparative examples 10-11 are shown in Table 13. The results of the regrowth and hair growth efficacy tests for samples from Examples 1-3, minoxidil, comparative examples 2-3, comparative examples 6-8, and comparative examples 10-11 after 12 weeks are shown in Table 14. Figure 13-15 These are comparison images of the samples from Example 1 used in human experiments before and after 12 weeks.

[0416] In Table 13, the efficacy of samples 1-3 was above 70%, higher than that of the minoxidil group. This indicates that the composition of the present invention has a highly effective hair growth effect. The efficacy of comparative samples 2, 3, and 8 was 27%, 33%, and 30%, respectively, significantly weaker than that of samples 1-3. Compared to samples 1-3 containing 7.5%, 10%, and 8% sodium lauryl sulfate, respectively, comparative samples 2, 3, and 8 containing 2.5%, 2.5%, and 7.3% sodium lauryl sulfate, this indicates that the hair growth effect of the composition containing 2.5%, 2.5%, and 7.3% sodium lauryl sulfate is weaker than that containing 7.5%, 10%, and 8%. The efficacy of comparative samples 6, 7, 10, and 11 was 43%, 40%, 33%, and 47%, respectively, significantly weaker than that of samples 1-3.

[0417] Table 14 clearly shows that the use of samples from Examples 1-3 significantly improved hair loss prevention compared to comparative samples 2-3, 6-8, and 10-11. Using samples from Examples 1-3 combined with minoxidil treatment resulted in hair loss levels comparable to normal adults. A normal adult loses an average of 50-100 hairs per day, with 6-10 hairs lost in specific areas. The table shows that after using samples from Examples 1-3, the skin condition remained normal for 12 weeks without any discomfort. This indicates that the hair follicles were in a dynamically stable state after using the composition, and that micro-damage and repair occurred simultaneously—that is, micro-damage activated hair follicle stem cells while anti-inflammatory repair occurred concurrently, maintaining hair follicle homeostasis and promoting efficient hair growth.

[0418] The difference between Comparative Example 2 and Examples 1-3 is the addition of 2.5 kg of sodium lauryl sulfate, and the absence of camel thorn leaf extract, selaginella extract, black pepper extract, green coffee bean extract, rosehip oil, macadamia nut oil, sphingomyelin, and copper peptides. Comparing the effects of Comparative Example 2 with those of Examples 1-3, the anti-hair loss effect was significantly weaker. The 2.5% sodium lauryl sulfate in the composition makes it difficult to activate hair follicle stem cells through micro-damage, thereby enhancing hair regrowth.

[0419] The difference between Comparative Example 3 and Examples 1-3 is the addition of 2.5 kg of sodium lauryl sulfate. Comparing the effects of Comparative Example 3 with Examples 1-3, the anti-hair loss effect was significantly weaker. The 2.5% sodium lauryl sulfate in the composition makes it difficult to activate hair follicle stem cells through micro-damage, thus hindering hair regrowth. This indicates that the composition can achieve highly efficient hair regrowth by activating hair follicle stem cells through micro-damage, as well as by activating and protecting them through free monounsaturated fatty acids and β-carotene; it also shows that activating hair follicle stem cells solely through free monounsaturated fatty acids and β-carotene is less effective. Furthermore, it indicates that without a synergistic effect of damage and repair in the composition, the hair regrowth effect is generally limited. When micro-damage and repair are performed simultaneously—that is, micro-damage activation of hair follicle stem cells and anti-inflammatory repair occur simultaneously—highly efficient hair regrowth can be achieved while maintaining hair follicle homeostasis. This demonstrates that simultaneous micro-damage and repair has a more efficient hair regrowth effect; micro-damage activation of hair follicle stem cells, combined with the activation of hair follicle stem cells by free monounsaturated fatty acids and β-carotene, has a synergistic effect.

[0420] The difference between Comparative Example 6 and Examples 1-3 is the absence of macadamia nut oil and rosehip oil. Macadamia nut oil contains monounsaturated fatty acids, and rosehip oil contains β-carotene. When the composition lacks free monounsaturated fatty acids and β-carotene, the hair regrowth effect is weak, indicating that a portion of free monounsaturated fatty acids and β-carotene is needed to enhance the hair regrowth effect. Activating hair follicle stem cells solely through micro-damage has a weak hair regrowth effect. This demonstrates that in this invention, activating hair follicle stem cells through micro-damage, combined with activating them through free monounsaturated fatty acids and β-carotene, achieves a highly efficient hair regrowth effect; simultaneously, β-carotene has a protective effect on hair follicles. This also indicates that in this invention, the activation of hair follicle stem cells through micro-damage, the activation of hair follicle stem cells through free monounsaturated fatty acids, and the activation of hair follicle stem cells through β-carotene have a synergistic enhancing effect.

[0421] The difference between Comparative Example 7 and Examples 1-3 is the absence of copper peptide, black pepper extract, and Selaginella extract. Selaginella extract contains paclitaxel flavonoids and dihydromyricetin. Dihydromyricetin has a novel, mild penetration-enhancing effect, possessing anti-inflammatory and antioxidant properties, and is non-irritating, making it a penetration enhancer specifically for sensitive skin. Black pepper extract contains piperine, which enhances the transdermal absorption of polyphenols. Copper peptide promotes transdermal absorption. Comparing the effects of Comparative Example 7 with those of Examples 1-3, the anti-hair loss effect was weaker. The hair regrowth effect of Comparative Example 7 compared to Examples 1-3 indicates that Selaginella extract, black pepper extract, and copper peptide in the composition promote transdermal absorption and enhance the transdermal effect of the active ingredients.

[0422] The difference between Comparative Example 8 and Examples 1-3 is the addition of 7.3 kg of sodium lauryl sulfate. Comparing the effects of Comparative Example 8 with Examples 1-3, the anti-hair loss effect was significantly weaker. Comparing the effects of Comparative Example 8 with Comparative Example 3, the anti-hair loss effects were similar. The composition containing 7.3% sodium lauryl sulfate did not achieve a hair follicle growth enhancement effect, indicating that it did not activate hair follicle stem cells through micro-damage. This suggests that the composition can achieve a highly efficient hair follicle growth effect by activating hair follicle stem cells through micro-damage, as well as through the activation of free monounsaturated fatty acids and β-carotene; it also indicates that the effect of activating hair follicle stem cells solely through free monounsaturated fatty acids and β-carotene is weak. It also suggests that when the composition only exerts anti-inflammatory and antioxidant effects, the hair follicle growth effect is generally limited. Furthermore, it indicates that a concentration of 7.5% or higher of sodium lauryl sulfate is needed in the composition to activate hair follicle stem cells through micro-damage and enhance the effect.

[0423] The difference between Comparative Example 10 and Examples 1-3 is that the latter used plant extracts that were not subjected to nanofiltration membrane separation. Comparing the effects of Comparative Example 10 with Examples 1-3, the anti-hair loss effect was significantly weaker. Camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, and rosehip oil extracted by ordinary alcohol extraction methods contain high proportions of crude fiber, crude protein, crude fat, and polysaccharides. These crude fibers, proteins, fats, and polysaccharides hinder skin permeation, preventing small molecules from passing through the stratum corneum, reducing the efficiency of small molecule permeation, and weakening the hair growth efficacy of the small molecule active ingredients in the composition. This indicates that nanofiltration controls the molecular weight of compounds in the plant extracts, enhancing the permeability of the active ingredients and thus achieving a highly effective hair growth effect. This demonstrates that camel thorn leaf extract, macadamia nut oil, selaginella extract, black pepper extract, green coffee bean extract, and rosehip oil obtained through microwave-membrane separation or pressing-membrane separation methods have stronger permeability, resulting in a highly effective hair growth effect.

[0424] Figure 6 The particle size distribution of the sample from Example 1 is shown in the figure. It can be observed that the particle size of the sample from Example 1 is concentrated between 100-300 nanometers, indicating that the sample from Example 1 is a nanoemulsion. The Tyndall effect of the prepared sample from Example 1 is as follows... Figure 7 The obvious Tyndall effect indicates that the sample in Example 1 is a nanoemulsion. Figure 8 This is a photograph of the actual packaging of the sample from Example 1. The Tyndall effect of the sample from Comparative Example 11 is as follows: Figure 9The absence of the Tyndall effect indicates that Comparative Example 11 is not an emulsion. The difference between Comparative Example 11 and Example 1 is the absence of sphingomyelin, suggesting that the added sphingomyelin has a nano-emulsifying effect. This indicates that in this invention, the nano-emulsifying effect of sphingomyelin helps enhance hair growth. Simultaneously, sphingomyelin decomposes into ceramides in the skin, which can regulate cell proliferation, anti-aging, anti-inflammation, and enhance skin repair.

[0425] The hair regrowth effects of samples 2, 3, 6, and 8 compared to those of samples 1-3 indicate that the 7.5-10% sodium lauryl sulfate in the composition can enhance hair regrowth through micro-damage to the skin. In the composition, sphingomyelin, camel thorn leaf extract, macadamia nut oil, selaginella extract, and green coffee bean extract have antioxidant and anti-inflammatory effects. This also demonstrates that the 7.5-10% sodium lauryl sulfate, sphingomyelin, camel thorn leaf extract, macadamia nut oil, selaginella extract, and green coffee bean extract in the composition of this invention can achieve highly efficient hair regrowth while maintaining hair follicle homeostasis. In this invention, the 7.5-10% sodium lauryl sulfate induces mild inflammation through micro-damage, promoting the secretion of monounsaturated fatty acids in the skin, thereby activating hair follicle stem cells and achieving a hair regrowth effect. The macadamia nut, rosehip oil, and 7.5-10% sodium lauryl sulfate added to the composition can activate hair follicle stem cells both through micro-damage and through the activation of hair follicle stem cells by free monounsaturated fatty acids and β-carotene in the composition, working synergistically to achieve highly efficient hair regrowth. Furthermore, the composition not only effectively activates hair follicle stem cells, but also protects hair follicles.

[0426] In this invention, the hair regrowth effects of Comparative Examples 10 and 11 samples compared to those of Examples 1-3 are due to the addition of sphingomyelin, which forms a nano-emulsion during emulsification. Nano-sized sphingomyelin more easily penetrates the stratum corneum of the skin. In the skin, sphingomyelin decomposes into ceramides, which can regulate cell proliferation, anti-aging, and anti-inflammation. The sphingomyelin nanoemulsion enhances the skin permeability of the active ingredients, thus enhancing the hair regrowth effect. The composition controls the molecular weight of compounds in the plant extracts through nanofiltration, enhancing the skin permeability of the active ingredients and achieving a highly efficient hair regrowth effect. The composition also enhances the hair regrowth effect by activating hair follicles through micro-damage. The dihydromyricetin, piperine, and copper peptides contained in the composition enhance the skin permeability of the active ingredients. These combined effects result in excellent hair regrowth.

[0427] In summary, the invention demonstrates that multiple synergistic effects work together to achieve highly efficient hair regrowth.

[0428] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A hair growth composition with multiple synergistic effects, characterized in that, The composition comprises the following components in parts by weight: 7.5-10 parts by weight of sodium lauryl sulfate, 8.4-16.1 parts by weight of plant extracts, 0.01-0.1 parts by weight of copper peptides, and 0.5-1.5 parts by weight of coenzyme Q10. 10 The plant extract comprises the following components by weight: 3-10 parts copidrome, 0.5-5 parts ammonium chloride, 1.5-3.5 parts adenosine, 2.2-3.3 parts nicotinamide, 0.3-1 parts sphingomyelin, 0.5-0.8 parts preservative, 27-38 parts permeabilizer, and 26-48.59 parts buffer solution; the plant extract further comprises the following components by weight: 3.2-5.3 parts camel thorn leaf extract, 3.6-6.4 parts macadamia nut oil, 0.3-1 parts selaginella extract, 0.3-1 parts black pepper extract, 0.5-1.1 parts green coffee bean extract, and 0.5-1.3 parts rosehip oil; the plant extract is subjected to nanofiltration separation.

2. The hair growth composition with multiple synergistic effects according to claim 1, characterized in that, The preparation steps of the plant extract are as follows: After crushing the plant, extractant A is added, followed by microwave treatment or pressing to obtain plant extract; the plant extract is then subjected to nanofiltration to obtain plant isolate; the plant isolate is concentrated by rotary evaporation or high-speed centrifugation, and then dried to obtain plant extract. The plants mentioned include camel thorn leaves, macadamia nuts, selaginella, black pepper, green coffee beans, and rosehips; the extractant A is selected from one or more of ethanol, diethyl ether, ethyl acetate, propylene glycol, and butylene glycol; the microwave treatment is performed by using microwaves at 2300-2500 MHz at 50-55 degrees Celsius for 15-25 minutes; the pressing treatment is performed by using a hydraulic press at 45-55 degrees Celsius for 0.5-1 hours; the rotary evaporation concentration is performed by rotary evaporation at 45-55 degrees Celsius and -1 to -0.9 MPa for 5-8 hours; the high-speed centrifugal concentration is performed by centrifuging at 10000-13000 r / min for 1-1.5 hours; and the drying is performed by drying at 50-55 degrees Celsius for 72-80 hours.

3. The hair growth composition with multiple synergistic effects according to claim 1, characterized in that, The nanofiltration separation process includes the following steps: The plant extract was filtered through a 50-200 mesh metal filter, and then through a 5-micron polypropylene membrane to obtain filtrate 1. Filtrate 1 was then filtered through a 0.1-1 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 2. Filtrate 2 was then filtered through a 0.01-0.05 micron polyvinylidene fluoride membrane or an inorganic ceramic membrane to obtain filtrate 3. Filtrate 3 was then filtered through a 0.66 nanometer polyvinylidene fluoride membrane or an inorganic ceramic nanofiltration membrane to obtain filtrate 4. Filtrate 4 is the plant isolate. The metal filter screen is selected from copper, silver, and stainless steel.

4. The hair growth composition with multiple synergistic effects according to claim 1, characterized in that, The preservative is one or more of phenoxyethanol, ethylhexylglycerin, and ethanol; the penetrant is one or more of ethanol, propylene glycol, butylene glycol, and PEG-40; the buffer solution is a mixture of sodium citrate and citric acid, with a weight ratio of citric acid to sodium citrate of 1.1-11.5:

1.

5. A method for preparing a hair growth composition with multiple synergistic effects according to any one of claims 1-4, characterized in that, Includes the following steps: Preparation of the alcohol phase liquid: 3.2-5.3 parts by weight of camel thorn leaf extract, 0.3-1 parts by weight of selaginella extract, 0.3-1 parts by weight of black pepper extract, 0.5-1.1 parts by weight of green coffee bean extract, 0.5-1.3 parts by weight of rosehip oil, 1.5-5 parts by weight of copiolite, and 0.3-1 parts by weight of sphingomyelin were placed in 27-38 parts by weight of permeabilizer and stirred at 45-57 degrees Celsius for 0.5-1 h to obtain the alcohol phase liquid; Preparation of aqueous phase liquid: 7.5-10 parts by weight of sodium dodecyl sulfate, 0.5-5 parts by weight of amnesic acid, 1.5-3.5 parts by weight of adenosine, 2.2-3.3 parts by weight of nicotinamide, and 0.5-0.8 parts by weight of preservative are placed in 26-48.59 parts by weight of buffer solution and stirred at 60-70 degrees Celsius for 0.5-1 h. Then, 1.5-5 parts by weight of cobilol are added and stirred for 0.6-0.8 h to obtain aqueous phase liquid. Preparation of the hair growth and development composition: While stirring at 800-2000 r / min, inject the alcohol phase liquid (45-57°C) into the aqueous phase liquid (60-70°C), emulsify at 6000-7000 r / min for 5-10 min, then place at 45-55°C and stir at 800-2000 r / min for 0.5-1 h. Next, add 3.6-6.4 parts by weight of macadamia nut oil and 0.5-1.5 parts by weight of coenzyme Q10. 10 Mix 0.01-0.1 parts by weight of blue copper peptide and stir at 45-55 degrees Celsius for 0.5-1 hours to obtain a hair growth composition.

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