A spray of a lactobacillus fermentation product and a method of making the same

Abstract

The application provides a lactobacillus fermentation product spray and a preparation method thereof, and belongs to the technical field of skin care cosmetics; the lactobacillus fermentation product spray comprises essential oil, rose water, butanediol, lactobacillus fermentation product, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, hydrolyzed sodium hyaluronate, ginseng leaf extract, dipropylene glycol, hydrolyzed collagen, damask rose extract, PCA sodium and PEG-50 hydrogenated castor oil; and the application also provides a preparation method of the lactobacillus fermentation product spray. Compared with the prior art, the application improves pores and reduces oil secretion by promoting skin metabolism and skin convergence through the compounding of components, has good moisturizing capacity, can moisturize the skin, has obvious bacteriostatic effect, can maintain skin homeostasis, and reduces the addition of preservatives.

Description

Technical Field

[0001] This invention belongs to the field of skin care and cosmetic technology, specifically relating to a lactobacillus fermentation product spray and its preparation method. Background Technology

[0002] Lactobacillus ferment precipitates have wide applications in the cosmetics industry. These products serve as natural bioactive ingredients, providing various benefits to skincare and cosmetic products. Lactobacillus ferment precipitates help maintain the skin's microecological balance, helping to prevent skin infections and inflammation. These products also contain various vitamins and antioxidants, which can promote skin cell renewal and repair while combating free radical damage. Simultaneously, Lactobacillus ferment precipitates act as effective moisturizers. Rich in lactic acid, these products promote the continuous renewal of dead skin cells on the skin's surface and improve skin hydration, making the skin smoother, softer, and more elastic. Lactobacillus ferment precipitates can also help improve skin tone. These products promote the continuous renewal of dead skin cells on the skin's surface and help eliminate skin blemishes and acne scars. They can also inhibit the activity of tyrosinase, thereby reducing melanin formation and making the skin whiter.

[0003] Therefore, lactobacillus fermentation products are widely used in the research and development and production of cosmetics. However, there is currently a lack of targeted compounding of other components in lactobacillus fermentation products besides lactic acid, resulting in unsatisfactory effects of the developed cosmetics.

[0004] CN116602910A discloses an oligopeptide composition and its application in the preparation of skin repair and anti-aging products. The oligopeptide composition contains 20-300 parts of Lactobacillus fermentation product filtrate, 10-100 parts of oligopeptide-1, 10-100 parts of palmitoyl pentapeptide-4, and 10-100 parts of acetyl tetrapeptide-9. The oligopeptide composition obtained by compounding Lactobacillus fermentation product filtrate as the main component only uses oligopeptide components to synergize with Lactobacillus fermentation product filtrate, and the effect is relatively simple.

[0005] CN114569537A discloses a skincare composition comprising one or more of a pre-cream, pre-lotion, pre-serum, facial cleanser, and eye cream, containing 0.5-1 parts of Sclerotium tsunemudo gum, 0.2-0.5 parts of sesame seed oil, 0.3-2 parts of saffron extract, 15-25 parts of Lactobacillus fermentation product extract, 15-20 parts of wild chrysanthemum extract, 1.5-15 parts of pomegranate peel extract, 0.5-5 parts of Tremella fuciformis extract, 2-8 parts of ascorbic acid (vitamin C), and 0.5-5 parts of sturgeon caviar extract. This invention, by adding sclerotium gum, sesame seed oil, saffron extract, wild chrysanthemum extract, pomegranate peel extract, tremella extract, ascorbic acid, and sturgeon caviar extract, can provide sufficient and effective nutritional supplements to the skin, thereby playing a corresponding skin care role. However, the skin care product composition includes products such as a base cream, base lotion, base serum, and cleansing cream, and uses a large number of active ingredients, resulting in too many functional components in the product, which can easily cause allergic reactions. Furthermore, the base lotion contains multiple lactobacillus fermentation products and also contains niacinamide, a component with poor stability under acidic conditions, which restricts the efficacy of each component in the product.

[0006] Therefore, the question arises as to how to provide a Lactobacillus fermentation product spray that can effectively leverage the advantages of Lactobacillus fermentation products, with a reasonable blend of components, to solve the problems of unreasonable cosmetic ingredient ratios, unsatisfactory oil control and moisturizing effects, excessive active ingredients, and added preservatives in existing technologies. Summary of the Invention

[0007] This invention addresses the problems of unreasonable ingredient ratios, unsatisfactory oil control and moisturizing effects, excessive active ingredients, and added preservatives in existing cosmetic technologies. It provides a lactobacillus fermentation product spray to simplify cosmetic ingredients, effectively improve oily areas of the face, keep the skin clean and refreshed, and provide excellent moisturizing capabilities to hydrate the skin.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A spray containing lactobacillus fermentation products, comprising the following components: Essential oils, rose (ROSA RUGOSA) flower water, butylene glycol, lactobacillus ferment filtrate, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, hydrolyzed sodium hyaluronate, centella asiatica (CENTELLA ASIATICA) leaf extract, dipropylene glycol, hydrolyzed collagen, ROSA DAMASCENA flower extract, sodium PCA, and PEG-50 hydrogenated castor oil.

[0009] Preferably, the lactobacillus fermentation product spray further includes the following components: Sodium chloride and pH adjuster.

[0010] More preferably, the pH adjuster comprises disodium hydrogen phosphate.

[0011] Preferably, the lactobacillus fermentation product spray comprises the following components in parts by weight percentage: Essential oil 0-0.05%, Rose (ROSA RUGOSA) flower water 0.5-5%, Butylene glycol 0.5-4%, Lactobacillus ferment 0.3-4%, 1,2-Pentanediol 0.05-1.5%, 1,2-Hexanediol 0.02-1.5%, p-Hydroxyacetophenone 0.01-0.9%, Hydrolyzed sodium hyaluronate 0.02-0.4%, Centella asiatica leaf extract 0.05-0.3%, Disodium hydrogen phosphate 0.01-0.1%, Sodium chloride 0.01-0.1%, Dipropylene glycol 0.01-0.1%, Hydrolyzed collagen 0.01-0.1%, Rosa damascena flower extract 0.01-0.1%, Sodium PCA 0.01-0.1%, and PEG-50 hydrogenated castor oil 0.01-0.1%.

[0012] More preferably, the lactobacillus fermentation product spray comprises the following components in parts by weight percentage: Essential oil 0-0.05%, Rose (ROSA RUGOSA) flower water 0.8-4.5%, Butylene glycol 0.9-3.5%, Lactobacillus ferment 1-4%, 1,2-Pentanediol 0.3-1.0%, 1,2-Hexanediol 0.3-1.0%, p-Hydroxyacetophenone 0.1-0.6%, Hydrolyzed sodium hyaluronate 0.1-0.3%, Centella asiatica leaf extract 0.1-0.2%, Disodium hydrogen phosphate 0.04-0.1%, Sodium chloride 0.05-0.1%, Dipropylene glycol 0.04-0.08%, Hydrolyzed collagen 0.04-0.08%, Rosa damascena flower extract 0.02-0.06%, Sodium PCA 0.02-0.06%, and PEG-50 hydrogenated castor oil 0.02-0.06%.

[0013] More preferably, the lactobacillus fermentation product spray comprises the following components in parts by weight percentage: Essential oil 0-0.05%, Rose (ROSA RUGOSA) flower water 2-4%, Butylene glycol 1.8-3.5%, Lactobacillus ferment 1.2-3.6%, 1,2-Pentanediol 0.5-0.8%, 1,2-Hexanediol 0.5-0.8%, p-Hydroxyacetophenone 0.2-0.5%, Hydrolyzed sodium hyaluronate 0.2-0.3%, Centella asiatica leaf extract 0.1-0.2%, Disodium hydrogen phosphate 0.06-0.08%, Sodium chloride 0.05-0.07%, Dipropylene glycol 0.05-0.07%, Hydrolyzed collagen 0.05-0.07%, Rosa damascena flower extract 0.04-0.06%, Sodium PCA 0.04-0.06%, and PEG-50 hydrogenated castor oil 0.04-0.06%.

[0014] Most preferably, the lactobacillus fermentation product spray comprises the following components in parts by weight percentage: The formula contains 0.02% essential oil, 3% rose (ROSA RUGOSA) flower water, 3% butylene glycol, 2.5% lactobacillus ferment filtrate, 0.7% 1,2-pentanediol, 0.6% 1,2-hexanediol, 0.4% p-hydroxyacetophenone, 0.3% hydrolyzed sodium hyaluronate, 0.1% centella asiatica leaf extract, 0.07% disodium hydrogen phosphate, 0.06% sodium chloride, 0.06% dipropylene glycol, 0.05% hydrolyzed collagen, 0.05% ROSA DAMASCENA flower extract, 0.04% sodium PCA, and 0.04% PEG-50 hydrogenated castor oil.

[0015] Essential oils include at least one of rose essential oil, lavender essential oil, chamomile essential oil, neroli essential oil, and geranium essential oil.

[0016] Rose (ROSA RUGOSA) floral water has the following effects: anti-oxidation, whitening and spot removal, and anti-aging; it has the effect of scavenging free radicals and can be used as an antioxidant to prevent aging; it can enhance cell activity and has a skin-revitalizing effect; it also has a certain inhibitory effect on tyrosinase, which can help with whitening.

[0017] Butylene glycol is commonly used as a moisturizer, solvent, skin conditioner, or viscosity control agent. It has moisturizing properties and also has certain antibacterial effects.

[0018] Lactobacillus species can ferment sugars to produce large amounts of lactic acid. Its fermentation product, nisin, has broad-spectrum antibacterial properties, strongly inhibiting both Gram-negative and Gram-positive bacteria, and possesses antibacterial and anti-inflammatory effects.

[0019] 1,2-Pentanediol is commonly used as a humectant, solubilizer, solvent, or skin conditioner; it has the excellent moisturizing properties of diols, as well as antibacterial and solubilizing effects, and can enhance the water resistance of sunscreen formulations.

[0020] 1,2-Hexanediol, also known as DL-1,2-hexanediol or DL-1,2-hexanediol, is a colorless, transparent, viscous liquid that can be mixed with a variety of organic compounds in any proportion. It is readily soluble in water and can be used as a preservative in contact with the human body, possessing both antibacterial and moisturizing properties.

[0021] p-Hydroxyacetophenone is a natural plant extract found naturally in the stems and leaves of *Artemisia capillaris* (Asteraceae family), and the roots of plants such as *Artemisia capillaris*, *Asclepiadaceae*, and *Gynostemma pentaphyllum*. It has the effects of clearing heat and dampness, promoting bile secretion and reducing jaundice. It is a preservative accelerator with multiple functions including antioxidant and anti-irritant properties. Its antioxidant and antibacterial properties come from the hydroxyl group, making it an antioxidant (phenolic and ketone characteristics). It is effective against fungi, has a strong killing effect on *Aspergillus niger*, and also has a certain inhibitory effect on *Pseudomonas aeruginosa*. It exhibits excellent stability across high and low pH and temperature ranges; it can extend the shelf life of other active ingredients under high temperature conditions and can promote the efficacy of various preservatives; it has good compatibility with various cosmetic ingredients and packaging materials.

[0022] Hydrolyzed sodium hyaluronate is an acidic mucopolysaccharide that can be used as a skin conditioning agent. It is naturally found in the corneal epithelium and can absorb 1000 times its own weight in water. It can retain skin moisture, prevent water loss through the epidermis, and play a barrier repair role when the skin is damaged. It makes the skin feel less dry after use, increases its radiance, improves skin nutrition and metabolism, makes the skin soft, smooth, wrinkle-free, more elastic, and prevents aging. While moisturizing, it is also a good transdermal absorption promoter.

[0023] Centella Asiatica leaf extract is commonly used as a moisturizer or skin conditioning agent, with moisturizing and antioxidant effects. It contains asiaticoside, which promotes the vitality of scar fibroblasts and has a good polymerization effect with phospholipids, thus having a significant effect on eliminating acne; it also has antibacterial properties.

[0024] The present invention also provides a method for preparing the above-mentioned Lactobacillus fermentation product spray, comprising the following steps: (1) The amount of hydrolyzed sodium hyaluronate in the formula was evenly dispersed in butanediol to obtain intermediate 1; (2) Mix the formulated amounts of PEG-50 hydrogenated castor oil, 1,2-pentanediol, 1,2-hexanediol and dipropylene glycol evenly to obtain intermediate 2; (3) Dissolve the prescribed amounts of sodium PCA and sodium chloride in the prescribed amounts of water to obtain intermediate 3; (4) Add intermediate 1 obtained in step (1) to intermediate 3 obtained in step (3), disperse, and obtain intermediate 4; (5) Add the prescribed amounts of rose (ROSA RUGOSA) flower water, lactobacillus fermentation product, p-hydroxyacetophenone, centella asiatica leaf extract, disodium hydrogen phosphate, hydrolyzed collagen and ROSA DAMASCENA flower extract to intermediate 4 obtained in step (4), stir, and obtain intermediate 5. (6) Add intermediate 2 obtained in step (2) to intermediate 5 obtained in step (5), stir, bottle and flush with propellant to obtain the Lactobacillus fermentation product spray.

[0025] Preferably, the propellant in step (6) is nitrogen.

[0026] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a compound based on Lactobacillus fermentation products. By leveraging the lactic acid in the fermentation products and combining it with hydrolyzed sodium hyaluronate, it reduces irritation and improves the refreshing feel while enhancing the penetration of lactic acid and other active ingredients (p-hydroxyacetophenone, Centella asiatica leaf extract, and Rosa damascena flower extract). This improves oily areas of the face, keeping the skin clean and minimizing pores. It also incorporates polyols with excellent moisturizing capabilities to hydrate the skin. Furthermore, this invention employs various antibacterial natural ingredients, ensuring product stability without the use of irritating preservatives.

[0027] In summary, compared with the prior art, the present invention improves pores and reduces sebum secretion through a compound of components that promote skin metabolism and tighten the skin, thus exhibiting good moisturizing ability and moisturizing the skin; and has a significant antibacterial effect, which can maintain skin homeostasis. Detailed Implementation

[0028] It is worth noting that the raw materials used in this invention are all commercially available products, and their sources are not specifically limited.

[0029] The CAS numbers for the following products are specified in this invention: Butanediol (CAS number 25265-75-2), 1,2-pentanediol (CAS number 5343-92-0), 1,2-hexanediol (CAS number 6920-22-5), p-hydroxyacetophenone (CAS number 99-93-4), hydrolyzed sodium hyaluronate (CAS number 9067-32-7), disodium hydrogen phosphate (CAS number 7558-79-4), sodium chloride (CAS number 7647-14-5), dipropylene glycol (CAS number 25265-71-8), hydrolyzed collagen (CAS number 92113-31-0), sodium PCA (CAS number 28874-51-3), and PEG-50 hydrogenated castor oil (CAS number 61788-85-0).

[0030] Rose essential oil, purchased from Shandong Huinong Rose Co., Ltd., product number HM-MD-001 (Pingyin double-petal red rose single essential oil), or can be replaced by Gansu Kushui Rose Industry Co., Ltd., product number KS-YJ-029 (Kushui rose essential oil).

[0031] Rose (ROSA RUGOSA) floral water, purchased from Shandong Huamei Biotechnology Co., Ltd., product number HM-CS-105 (RUGOSA double-petal rose floral water); Centella Asiatica leaf extract, purchased from Guilin Rhine Biotechnology Co., Ltd., product number LAYN-JXC-803 (Centella Asiatica leaf extract, cosmetic grade), or can be replaced by Xi'an Lvtian Biotechnology Co., Ltd. (focusing on Centella Asiatica monomer raw materials), product number LT-JXC-612.

[0032] Hydrolyzed collagen was purchased from Baotou Dongbao Biotechnology Co., Ltd., product number DB-CL-908 (small molecule hydrolyzed collagen, cosmetic use), or it can be replaced by Bloomage Biotechnology Co., Ltd., product number HX-JY-771; Rosa damascena flower extract, purchased from Shanghai Zhina Biotechnology Co., Ltd. (a leading domestic manufacturer of Damask rose raw materials), product number DF410-D008 (Rosa damascena flower extract stock solution). The lactobacillus fermentation product in this invention is the fermentation supernatant of Bifidobacterium lactis with accession number CGMCC NO.24307. The fermentation conditions are 37±1℃, anaerobic, and cultured for 72±3h. Specifically, it can be prepared according to Example 1 and Example 2 of CN115317432B. The Bifidobacterium lactis CGMCC NO.24307 can be purchased from China General Microbiological Culture Collection Center or Shenzhen Duoweisheng Health Food Co., Ltd.

[0033] The Escherichia coli strain used in the following examples and control examples is CICC 10899, and the Staphylococcus aureus strain is CICC 21600, both of which were purchased from the China Industrial Microbial Culture Collection Center.

[0034] Examples 1-5: A spray of Lactobacillus fermentation product and its preparation method The components and percentage by weight of the Lactobacillus fermentation product sprays in Examples 1-5 are shown in the table below: Table 1

[0035] The preparation methods of the Lactobacillus fermentation product spray in Examples 1-5 are as follows: (1) The amount of hydrolyzed sodium hyaluronate in the formula was evenly dispersed in butanediol to obtain intermediate 1; (2) Mix the formulated amounts of PEG-50 hydrogenated castor oil, 1,2-pentanediol, 1,2-hexanediol and dipropylene glycol evenly to obtain intermediate 2; (3) Dissolve the prescribed amounts of sodium PCA and sodium chloride in the prescribed amounts of water to obtain intermediate 3. (4) Add intermediate 1 obtained in step (1) to intermediate 3 obtained in step (3), disperse, and obtain intermediate 4; (5) Add the formulated amounts of rose essential oil, rose (ROSA RUGOSA) flower water, lactobacillus fermentation product, p-hydroxyacetophenone, centella asiatica leaf extract, disodium hydrogen phosphate, hydrolyzed collagen and ROSA DAMASCENA flower extract to intermediate 4 obtained in step (4), stir, and obtain intermediate 5; (6) Add intermediate 2 obtained in step (2) to intermediate 5 obtained in step (5), stir, bottle and flush with propellant (nitrogen) to obtain the Lactobacillus fermentation product spray.

[0036] Comparative Examples 1-2 The only difference between Comparative Examples 1-2 and Example 1 is the amount of raw materials used, as shown in Table 1.

[0037] Comparative Example 3 The only difference between this comparative example and Example 1 is that the Centella Asiatica leaf extract was replaced with an equal amount of Centella Asiatica extract, and the Rosa Damascena flower extract was replaced with an equal amount of Rosa Damascena extract.

[0038] Comparative Example 4 The only difference between this comparative example and Example 1 is that the Centella Asiatica leaf extract was replaced with an equal amount of p-hydroxyacetophenone.

[0039] Comparative Example 5 The only difference between this comparative example and Example 1 is that p-hydroxyacetophenone was replaced with an equal amount of Centella Asiatica leaf extract.

[0040] Experimental Example 1: Short-term skin sebum secretion test The SEBUMETER® method was used for testing.

[0041] We are recruiting 80 volunteers, aged 20 to 40, with no gender restrictions. Volunteers must meet the following requirement: their sebum level must be 150 μg / cm³ or higher three hours after cleansing their face in the morning. 2 .

[0042] The volunteers were then randomly divided into 10 groups. During the experiment, a half-face test was used for comparison. Volunteers first washed their faces and then sat quietly for 20 minutes in a constant temperature and humidity environment (temperature: 25±1℃, relative humidity: 40±5%). The forehead area was divided into left and right halves, with the center of the eyebrows as the boundary, and the skin oil content of each half was measured.

[0043] Subsequently, we sprayed appropriate amounts of the Lactobacillus fermentation products prepared in the examples and comparative examples onto the forehead test areas of different groups of volunteers (corresponding one-to-one with Examples 1-5 and Comparative Examples 1-5), and waited for them to dry. Data was recorded before spraying. One hour, two hours, and three hours after sample use, the changes in sebum secretion on the left and right sides of the face were measured and recorded using the same method. In all the above measurements, each test area was tested three times, and the average value was taken as the experimental result. The average value of each group of volunteers was taken as the experimental result for each example / comparative example. The results are shown in Table 2, with units of μg / cm³. 2 : Table 2

[0044] As shown in Table 2, the Lactobacillus fermentation product sprays in Examples 1-5 of this invention exhibited changes in skin sebum secretion ranging from 4.13 to 6.81 μg / cm³ during a 3-hour short-term skin sebum secretion test. 2 The values ​​were between 12.33 μg / cm³; while comparative examples 1-5 were all between 12.33 μg / cm³. 2 The highest concentration can reach 45.28 μg / cm³. 2 Comparing Example 1 with the comparative examples, it can be seen that: Comparative Examples 1-2 have different amounts of each raw material compared to Example 1, and the ratio is unreasonable, resulting in higher short-term sebum secretion in the skin. The chemical composition of Comparative Example 3 changed significantly due to the replacement of Centella Asiatica / Rosa damascena extract at specific locations, which in turn reduced the oil control effect of the Lactobacillus fermentation product spray prepared in Comparative Example 3. In Comparative Examples 4 and 5, the compound components were replaced with single components, which prevented the synergistic effect of Centella asiatica leaf extract and p-hydroxyacetophenone from being exerted, resulting in a short-term skin sebum secretion of 23.01 μg / cm³ after 3 hours. 2 The above is significantly higher than the 4.13 μg / cm³ of Example 1. 2 .

[0045] Experimental Example 2 Eighty volunteers (female, aged 25-35) were recruited and divided into 10 groups of 8 people each. The experiment was conducted in winter, with the first test at 8:00 am. The test site was the face. Skin moisture content was measured before spraying the products prepared in Examples 1-5 and Comparative Examples 1-5 and at 0h, 6h, 12h, and 24h after using the products prepared in Examples 1-5 and Comparative Examples 1-5. The test results were averaged and expressed as a percentage. The results are shown in Table 3.

[0046] Table 3

[0047] It is evident that the Lactobacillus fermentation product spray provided by this invention still maintains the skin's moisture content well 12 hours after spraying, effectively moisturizing the skin.

[0048] Experimental Example 3 Antibacterial activity test: Referring to section 7.3 of QB / T 2738-2012 "Evaluation Methods for Antibacterial and Antimicrobial Effects of Daily Chemical Products" (Suspension Quantitative Method), the antibacterial effects of the Lactobacillus fermentation product sprays obtained in Examples 1-5 and Comparative Examples 1-5 of this application on Escherichia coli and Staphylococcus aureus were tested. The test results are shown in Table 4, with the unit being the inhibition rate (%), and the contact time being 20 min.

[0049] Table 4

[0050] As shown in Table 4, the products in the examples have antibacterial effects against Escherichia coli and Staphylococcus aureus, while the products in the comparative examples have no antibacterial effect against these two bacteria.

[0051] Specifically, the antibacterial efficacy against Escherichia coli in Examples 1, 2, 3, 4, and 5 was all above 65.1%, and the antibacterial efficacy against Staphylococcus aureus was all above 56.7%. These data indicate that the products in the examples have good antibacterial effects against both of these bacteria.

[0052] However, the antibacterial effect of the comparative samples against the two bacteria mentioned above was less than 50%, which does not indicate that they have a significant antibacterial effect.

[0053] In summary, the Lactobacillus fermentation product spray provided in Examples 1-5 of this invention has good oil control performance, which is due to the addition of specific components and dosages of moisturizing components and skin conditioners; at the same time, it has a significant antibacterial effect, reduces the use of preservatives, and can also ensure skin homeostasis.

[0054] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A spray containing lactobacillus fermentation products, characterized in that, Includes the following components: Essential oils, rose water, butylene glycol, lactobacillus ferment filtrate, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, hydrolyzed sodium hyaluronate, centella asiatica leaf extract, dipropylene glycol, hydrolyzed collagen, Rosa damascena flower extract, sodium PCA, and PEG-50 hydrogenated castor oil.

2. The Lactobacillus fermentation product spray according to claim 1, characterized in that, It also includes the following components: Sodium chloride and pH adjuster.

3. The Lactobacillus fermentation product spray according to claim 2, characterized in that, The pH adjuster includes disodium hydrogen phosphate.

4. The Lactobacillus fermentation product spray according to claim 3, characterized in that, The components include the following percentages by weight: The ingredients include: essential oil 0-0.05%, rose water 0.5-5%, butylene glycol 0.5-4%, Lactobacillus ferment 0.3-4%, 1,2-pentanediol 0.05-1.5%, 1,2-hexanediol 0.02-1.5%, p-hydroxyacetophenone 0.01-0.9%, hydrolyzed sodium hyaluronate 0.02-0.4%, Centella asiatica leaf extract 0.05-0.3%, disodium hydrogen phosphate 0.01-0.1%, sodium chloride 0.01-0.1%, dipropylene glycol 0.01-0.1%, hydrolyzed collagen 0.01-0.1%, Rosa damascena flower extract 0.01-0.1%, sodium PCA 0.01-0.1%, and PEG-50 hydrogenated castor oil 0.01-0.1%.

5. The Lactobacillus fermentation product spray according to claim 4, characterized in that, The product comprises the following components by weight percentage: essential oil 0-0.05%, rose water 0.8-4.5%, butylene glycol 0.9-3.5%, Lactobacillus ferment 1-4%, 1,2-pentanediol 0.3-1.0%, 1,2-hexanediol 0.3-1.0%, p-hydroxyacetophenone 0.1-0.6%, hydrolyzed sodium hyaluronate 0.1-0.3%, Centella asiatica leaf extract 0.1-0.2%, disodium hydrogen phosphate 0.04-0.1%, sodium chloride 0.05-0.1%, dipropylene glycol 0.04-0.08%, hydrolyzed collagen 0.04-0.08%, Rosa damascena flower extract 0.02-0.06%, sodium PCA 0.02-0.06%, and PEG-50 hydrogenated castor oil 0.02-0.06%.

6. The Lactobacillus fermentation product spray according to claim 5, characterized in that, The product comprises the following components by weight percentage: essential oil 0-0.05%, rose water 0.8-4.5%, butylene glycol 0.9-3.5%, Lactobacillus ferment 1-4%, 1,2-pentanediol 0.3-1.0%, 1,2-hexanediol 0.3-1.0%, p-hydroxyacetophenone 0.1-0.6%, hydrolyzed sodium hyaluronate 0.1-0.3%, Centella asiatica leaf extract 0.1-0.2%, disodium hydrogen phosphate 0.04-0.1%, sodium chloride 0.05-0.1%, dipropylene glycol 0.04-0.08%, hydrolyzed collagen 0.04-0.08%, Rosa damascena flower extract 0.02-0.06%, sodium PCA 0.02-0.06%, and PEG-50 hydrogenated castor oil 0.02-0.06%.

7. The Lactobacillus fermentation product spray according to claim 6, characterized in that, The product comprises the following components by weight percentage: essential oil 0-0.05%, rose water 2-4%, butylene glycol 1.8-3.5%, Lactobacillus ferment 1.2-3.6%, 1,2-pentanediol 0.5-0.8%, 1,2-hexanediol 0.5-0.8%, p-hydroxyacetophenone 0.2-0.5%, hydrolyzed sodium hyaluronate 0.2-0.3%, Centella asiatica leaf extract 0.1-0.2%, disodium hydrogen phosphate 0.06-0.08%, sodium chloride 0.05-0.07%, dipropylene glycol 0.05-0.07%, hydrolyzed collagen 0.05-0.07%, Rosa damascena flower extract 0.04-0.06%, sodium PCA 0.04-0.06%, and PEG-50 hydrogenated castor oil 0.04-0.06%.

8. The Lactobacillus fermentation product spray according to claim 7, characterized in that, The components include the following percentages by weight: The ingredients include 0.02% essential oil, 3% rose water, 3% butylene glycol, 2.5% Lactobacillus ferment precipitate, 0.7% 1,2-pentanediol, 0.6% 1,2-hexanediol, 0.4% p-hydroxyacetophenone, 0.3% hydrolyzed sodium hyaluronate, 0.1% Centella asiatica leaf extract, 0.07% disodium hydrogen phosphate, 0.06% sodium chloride, 0.06% dipropylene glycol, 0.05% hydrolyzed collagen, 0.05% Rosa damascena flower extract, 0.04% sodium PCA, and 0.04% PEG-50 hydrogenated castor oil.

9. A method for preparing the Lactobacillus fermentation product spray according to any one of claims 1-8, comprising the following steps: (1) The hydrolyzed sodium hyaluronate of the formula amount was evenly dispersed in butanediol to obtain intermediate 1; (2) Mix the formulated amounts of PEG-50 hydrogenated castor oil, 1,2-pentanediol, 1,2-hexanediol and dipropylene glycol evenly to obtain intermediate 2; (3) Dissolve the prescribed amounts of sodium PCA and sodium chloride in the prescribed amounts of water to obtain intermediate 3; (4) Add intermediate 1 obtained in step (1) to intermediate 3 obtained in step (3), disperse, and obtain intermediate 4; (5) Add the prescribed amounts of essential oil, rose water, lactobacillus fermentation product, p-hydroxyacetophenone, centella asiatica leaf extract, disodium hydrogen phosphate, hydrolyzed collagen and Rosa damascena flower extract to intermediate 4 obtained in step (4), stir, and obtain intermediate 5. (6) Add intermediate 2 obtained in step (2) to intermediate 5 obtained in step (5), stir, bottle and flush with propellant to obtain the Lactobacillus fermentation product spray.

10. The preparation method according to claim 9, characterized in that: The propellant mentioned in step (6) is nitrogen.

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