A pipette tip, a pipette and a method of using a pipette

Abstract

The application relates to a pipette tip, a pipette and a method for using the pipette, the pipette tip comprising a liquid storage tube, a connecting piece and a coating rod. The liquid storage tube has a liquid storage cavity, and one end of the liquid storage tube forms a liquid suction port communicating with the liquid storage cavity. The connecting piece is arranged at an angle with the liquid storage tube, one end of the connecting piece is connected to the liquid storage tube at a position adjacent to the liquid suction port, the other end of the connecting piece extends in a direction away from the liquid suction port, the coating rod is connected to the end of the connecting piece away from the liquid storage tube, and the length direction of the coating rod is at an angle with the axis direction of the liquid storage tube. A bypass channel is arranged in the connecting piece, the coating rod is provided with a plurality of liquid distribution holes, the liquid storage tube is provided with a bypass through hole, the liquid storage cavity communicates with the bypass channel, a steel ball is arranged in the bypass channel, the steel ball is used for selectively plugging the bypass through hole, and the liquid distribution holes all communicate with the bypass channel. The pipette tip can integrate pipetting and coating together, improve operation efficiency, reduce the risk of being polluted by the outside world, and improve coating uniformity.

Description

Technical Field

[0001] This invention relates to the field of pipette technology, and more particularly to a pipette tip, a pipette, and a method of using the pipette. Background Technology

[0002] A pipette, also called a pipette tip, is a measuring tool used to transfer liquid from one container to another (such as a petri dish) within a certain volume range. It is widely used in biology, chemistry, and other fields, and is commonly used for the aspiration, transfer, dilution, distribution, and mixing of small or trace amounts of liquid. In related techniques, when using the spread plating method for bacterial colony counting, a certain volume of liquid needs to be aspirated with a pipette and transferred to the culture medium, and then a sterile spreader is used to evenly spread the liquid. During this process, after transferring the liquid, the pipette tip needs to be removed and the corresponding spreader replaced. This method is not only slow and time-consuming, but also prone to contact with contaminants from the external environment (such as personnel's limbs, packaging boxes, etc.) during the spreader replacement process, thus failing to meet the aseptic requirements of the operation. Summary of the Invention

[0003] The purpose of this invention is to provide a pipette tip, a pipette, and a method for using the pipette, which integrates pipetting and coating, improves work efficiency, reduces the risk of external contamination, and enhances coating uniformity.

[0004] To achieve this objective, the present invention adopts the following technical solution: A pipette tip is provided, comprising a reservoir tube, a connector, and a coating rod. The reservoir tube has a reservoir cavity, and one end of the reservoir tube forms a suction port communicating with the reservoir cavity. The connector is disposed at an angle to the reservoir tube, with one end connected to the reservoir tube near the suction port and the other end extending away from the suction port. The coating rod is connected to the end of the connector away from the reservoir tube, and the length direction of the coating rod forms an angle with the axial direction of the reservoir tube. The connector is provided with a bypass channel, the coating rod is provided with a plurality of liquid dispensing holes at intervals along its length, the side wall of the liquid storage tube is provided with a bypass through hole, the liquid storage chamber is connected to the bypass channel through the bypass through hole, the bypass channel is provided with a freely rolling steel ball, the steel ball is used to selectively block the bypass through hole, and all the liquid dispensing holes are connected to the bypass channel.

[0005] As a preferred embodiment of the pipette tip, the length direction of the coating rod is perpendicular to the axial direction of the reservoir tube.

[0006] As a preferred embodiment of the pipette tip, the connector is attached to the middle of the coating rod along its length.

[0007] As a preferred embodiment of the pipette tip, along the length of the coating rod, the coating rod includes a straight section and a curved section connected to each other. The straight section has a cylindrical structure, and the curved section is bent toward the side of the reservoir tube at one end away from the straight section.

[0008] As a preferred embodiment of the pipette tip, the diameter of the curved section gradually decreases from the end near the straight section to the end away from the straight section.

[0009] As a preferred embodiment of the pipette tip, a liquid guiding groove is provided on the outer wall of the coating rod, and the liquid guiding groove is connected to the dispensing hole.

[0010] As a preferred embodiment of the pipette tip, the side wall of the reservoir tube is provided with a groove that fits the surface of the steel ball. The groove is connected to the bypass channel, and the bypass through hole is located at the bottom of the groove. When the reservoir tube is in a vertical state, the steel ball rolls into the groove and can block the bypass through hole.

[0011] As a preferred embodiment of the pipette tip, a filter element is provided in the liquid storage chamber. The filter element is located at the end of the liquid storage chamber away from the suction port, and the filter element is used to block the test liquid in the liquid storage chamber.

[0012] A pipette is also provided. As a preferred embodiment of a pipette tip, it includes a pipette body and a pipette tip, wherein the reservoir tube in the pipette tip is detachably connected to the pipette body.

[0013] A method for using a pipette is also provided, which involves transferring the test solution from the original container to the culture medium using the pipette. The method for using the pipette specifically includes the following steps: Step S1: Insert the pipette vertically into the original container, and insert the end of the storage tube with the suction port into the test solution. Position the coating rod above the surface of the test solution, and operate the pipette body to draw the test solution into the storage tube. Step S2: Drop the test solution onto the culture medium; Step S3: Adjust the pipette to an inclined position so that the spreading rod contacts the culture medium, and use the spreading rod to spread the test solution evenly.

[0014] The advantages of this invention compared to the prior art are: This invention discloses a pipette tip, a pipette, and a method for using the pipette. The pipette tip is used to draw a certain volume of test solution from the original container, then drop the test solution into the culture medium, and then spread the test solution evenly. By setting a spreading rod on the reservoir tube, pipetting and spreading are integrated. After pipetting, the operator can use the spreading rod to spread the test solution evenly without replacing the pipette tip. This shortens the operation time, improves the operation efficiency, and avoids contamination from contact with the external environment during replacement, thus meeting the aseptic requirements of the operation process. Furthermore, by setting a dispensing hole on the spreading rod, the test solution can be discharged through the dispensing hole and flow onto the surface of the spreading rod. When using the spreading rod for spreading, the test solution flows to the surface of the spreading rod through the dispensing hole, which can replenish the test solution in the spreading area during the spreading process and keep the spreading rod in a moist state, promoting the even distribution of the test solution on the culture medium. Attached Figure Description

[0015] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0016] Figure 1 This is a schematic diagram of a pipette tip according to an embodiment of the present invention.

[0017] Figure 2 This is a cross-sectional view of the liquid storage tube according to an embodiment of the present invention.

[0018] Figure 3 This is a cross-sectional view of the pipette tip in a vertical position according to an embodiment of the present invention.

[0019] Figure 4 This is a cross-sectional view of the pipette tip in an inclined state according to an embodiment of the present invention.

[0020] Figure 5 This is a schematic diagram of a pipette according to an embodiment of the present invention.

[0021] In the picture: 1. Liquid storage tube; 11. Liquid storage chamber; 12. Suction port; 13. Constant diameter section; 14. Variable diameter section; 15. Bypass through hole; 16. Scale line; 2. Connector; 21. Bypass channel; 22. Groove; 3. Coating rod; 31. Straight section; 32. Bending section; 33. Dispensing channel; 34. Dispensing hole; 35. Connecting hole; 36. Liquid guide groove; 4. Filter element; 5. Steel ball; 100. Pipette body; 110. Shell; 120. Piston assembly; 130. Connector. Detailed Implementation

[0022] The advantages and features of the present invention, as well as methods of implementing them, will become apparent from the following detailed description of the embodiments in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various different forms. These embodiments are provided merely to complete the disclosure of the invention and to enable those skilled in the art to fully understand the scope of the invention, which is defined only by the scope of the claims. The same reference numerals denote the same constituent elements throughout the specification.

[0023] The present invention will now be described in detail with reference to the accompanying drawings.

[0024] like Figure 1 , Figure 2 and Figure 5 As shown, this invention provides a pipette tip, including a reservoir tube 1, a connector 2, and a coating rod 3. The reservoir tube 1 is used to draw and temporarily store the test solution from the original container. The reservoir tube 1 has a reservoir cavity 11 for temporarily storing the test solution. One end of the reservoir tube 1 forms a suction port 12, which communicates with the reservoir cavity 11. The end of the reservoir tube 1 facing away from the suction port 12 is used to connect to the pipette body 100. The connector 2 is used to connect the reservoir tube 1 and the coating rod 3 together. The connector 2 has a long rod-like structure, with one end connected to the reservoir tube 1 and the other end connected to the coating rod 3. The connector 2 and the reservoir tube 1 are arranged at an angle, that is, the axis of the connector 2 forms an angle with the axis of the reservoir tube 1. This angled arrangement between the connector 2 and the reservoir tube 1 accommodates the relative position between the coating rod 3 and the reservoir tube 1. One end of connector 2 is connected to the reservoir tube 1 near the aspiration port 12, and the other end extends away from the aspiration port 12. This structure spacees the coating rod 3 from the reservoir tube 1, with the coating rod 3 located at the end of the reservoir tube 1 closest to the aspiration port 12. The coating rod 3 is used to evenly spread the test solution dropped into the culture medium. The coating rod 3 has a long rod-like structure, and the length direction (i.e., the axial direction) of the coating rod 3 forms an angle with the axial direction of the reservoir tube 1, so that the operator can achieve a wider coating area by moving the entire pipette tip back and forth along the axial direction of the reservoir tube 1.

[0025] A bypass channel 21 is provided inside the connector 2, and the length of the bypass channel 21 extends along the axial direction of the connector 2. A liquid distribution channel 33 is provided inside the coating rod 3, and the length of the liquid distribution channel 33 extends along the axial direction of the coating rod 3. A plurality of liquid distribution holes 34 are provided on the side wall of the coating rod 3, and the plurality of liquid distribution holes 34 are distributed at intervals along the axial direction of the coating rod 3. The liquid distribution holes 34 penetrate through the side wall of the coating rod 3, so that one end of the liquid distribution hole 34 communicates with the liquid distribution channel 33, and the other end communicates with the outside of the coating rod 3. A connecting hole 35 is also provided on the coating rod 3, which is located on the side wall of the coating rod 3 facing the connector 2, so that the liquid distribution channel 33 communicates with the bypass channel 21 through the connecting hole 35. A bypass through hole 15 is provided on the side wall of the liquid storage tube 1, and the liquid storage chamber 11 communicates with the bypass channel 21 through the bypass through hole 15. The storage chamber 11 is sequentially connected to the dispensing hole 34 via the bypass through-hole 15, bypass channel 21, connecting hole 35, dispensing channel 33, and dispensing hole 34. The test solution within the storage chamber 11 can be discharged to the outside of the coating rod 3 via the bypass through-hole 15, bypass channel 21, connecting hole 35, dispensing channel 33, and dispensing hole 34. A steel ball 5 is installed inside the bypass channel 21. When the operator adjusts the tilt angle of the connector 2, the steel ball 5 can roll freely under its own weight. The rolling of the steel ball 5 allows it to selectively block the bypass through-hole 15. For example, when the pipette tip is in a vertical position, the axis of the storage tube 1 extends vertically. At this time, the position of the bypass through-hole 15 is lower than that of the bypass channel 21. The steel ball 5 can roll freely to the end of the bypass channel 21 near the bypass through-hole 15, thus blocking the bypass through-hole 15. When the pipette tip is tilted, the spreading rod 3 is located below the reservoir tube 1 and can contact the culture medium. At this time, the bypass through hole 15 is higher than the bypass channel 21, and the steel ball 5 can roll freely to the end of the bypass channel 21 near the spreading rod 3. The reservoir 11 and the bypass channel 21 are connected through the bypass through hole 15.

[0026] Understandably, pipette tips are used to draw a certain volume of test solution from the original container, then drop the test solution into the culture medium, and then spread the test solution evenly. By setting a spreading rod 3 on the reservoir tube 1, pipetting and spreading are integrated together. After pipetting, the operator can use the spreading rod 3 to spread the test solution evenly without replacing the pipette tip. This shortens the operation time, improves efficiency, and avoids contamination of the spreading rod 3 from contact with the external environment during replacement, thus meeting the aseptic requirements of the operation process. Furthermore, by setting a dispensing hole 34 on the spreading rod 3, the test solution can be discharged through the dispensing hole 34 and flow onto the surface of the spreading rod 3. When using the spreading rod 3 for spreading, the test solution flows onto the surface of the spreading rod 3 through the dispensing hole 34, which can replenish the test solution in the spreading area during the spreading process and keep the spreading rod 3 in a moist state, promoting the even distribution of the test solution on the culture medium.

[0027] Specifically, the liquid storage tube 1 includes a constant-diameter section 13 and a variable-diameter section 14 connected sequentially along its axial direction. The constant-diameter section 13 has the same diameter, while the diameter of the variable-diameter section 14 gradually decreases from the end near the constant-diameter section 13 to the end away from it. A suction port 12 is located at the end of the variable-diameter section 14 opposite to the constant-diameter section 13. Alternatively, the end of the liquid storage tube 1 with the suction port 12 can be understood as having a tapered structure. The liquid storage tube 1 is made of a transparent material, such as glass or resin, so that the operator can observe the test liquid inside the liquid storage tube 1. Graduation lines 16 are provided on the outer wall of the constant-diameter section 13 to indicate the volume of the test liquid inside the liquid storage tube 1. One end of the connector 2 is connected to the variable-diameter section 14.

[0028] Specifically, the length direction of the coating rod 3 is perpendicular to the axis of the reservoir tube 1. During coating, the operator pushes the entire pipette tip back and forth along the axial direction of the reservoir tube 1. Because the length direction of the coating rod 3 is perpendicular to the direction of movement of the pipette tip, the coating rod 3 can cover a larger coating area for ease of use. Along the length direction of the coating rod 3, the end of the connector 2 facing away from the reservoir tube 1 is connected to the middle of the coating rod 3. This structure makes the center of gravity of the entire pipette tip closer to the axis of the reservoir tube 1, which is more conducive to the operator performing operations such as aspiration, dispensing, and coating. Along the length direction of the coating rod 3, the coating rod 3 includes a straight section 31 and a curved section 32 connected to each other. The axis of the straight section 31 is a straight line, and the axis of the curved section 32 is a curve. The straight section 31 has a cylindrical structure and is used to perform large-area coating operations. During coating, the entire length of the straight section 31 can contact the culture medium, resulting in a large coating area. The curved section 32 bends towards the reservoir tube 1 at the end opposite to the straight section 31. In this embodiment, the coating rod 3 and the reservoir tube 1 are located on opposite sides of the connector 2 along its length. The curved section 32 has a structure with one end larger than the other, and its diameter gradually decreases from the end near the straight section 31 to the end opposite to it. The smaller end of the curved section 32 bends towards the connector 2 near the reservoir tube 1. By setting the curved section 32, small-area coating operations can be performed. When small-area coating operations are required on the culture medium, the operator can rotate the pipette tip appropriately and use the curved section 32 for coating to reduce the contact area between the coating rod 3 and the culture medium. Furthermore, by setting the curved section 32 with one end larger than the other, the corner areas of the culture medium can be coated using the smaller end of the curved section 32. Therefore, this pipette tip can meet the needs of large-area coating, small-area coating, and corner coating in coating operations.

[0029] Specifically, refer to Figure 3 and Figure 4As shown, a groove 22 is provided on the side wall of the liquid storage tube 1. The groove 22 is located on the side wall of the liquid storage tube 1 facing the connector 2, and the groove 22 communicates with the bypass channel 21. The shape of the groove 22 matches the outer surface of the steel ball 5 so that part of the steel ball 5 can be accommodated in the groove 22 and fit against the groove 22. The opening of the groove 22 faces the bypass channel 21, and the bypass through hole 15 is provided at the bottom of the groove 22. When the liquid storage tube 1 is in a vertical state, the steel ball 5 rolls into the groove 22, and the outer surface of the steel ball 5 fits against the groove 22 to block the bypass through hole 15. It should be noted that the connector 2 is connected to the straight section 31 of the coating rod 3. The straight section 31 has a cylindrical structure, so that the side wall of the straight section 31 in the bypass channel 21 is an arc surface structure. When the steel ball 5 rolls to the end of the bypass channel 21 near the coating rod 3, the steel ball 5 abuts against the outer wall of the coating rod 3. Since both the surface of the steel ball 5 and the surface of the coating rod 3 are curved, the contact between the steel ball 5 and the coating rod 3 is point contact. The position of the connecting hole 35 should avoid the contact point between the steel ball 5 and the coating rod 3, so that the bypass channel 21 can be connected to the dispensing channel 33 through the connecting hole 35. In practical applications, when aspirating, the pipette tip is adjusted to a vertical position, and the bypass hole 15 is blocked by the steel ball 5. At this time, when the storage tube 1 is evacuated, the test liquid enters the storage chamber 11 from the suction port 12. During coating, the pipette tip is adjusted to an inclined position, and the bypass hole 15 is open. When the storage tube 1 is pressurized, the test liquid in the storage chamber 11 can be discharged simultaneously from the suction port 12 and the dispensing hole 34.

[0030] Specifically, refer to Figure 1 As shown, a liquid guiding groove 36 is provided on the outer wall of the coating rod 3. The liquid guiding groove 36 communicates with the dispensing hole 34, so that the test liquid flowing out of the dispensing hole 34 can be guided to the outer wall of the coating rod 3 through the liquid guiding groove 36 to promote the dispersion of the test liquid on the surface of the coating rod 3. The liquid guiding groove 36 includes a first groove and a second groove. The first groove extends along the axial direction of the coating rod 3, and the second groove extends along the circumferential direction of the coating rod 3. The first groove communicates with the dispensing hole 34, and the second groove intersects with and communicates with the first groove. In this embodiment, the first groove and the second groove interweave with each other to expand the distribution area of ​​the liquid guiding groove 36 on the surface of the coating rod 3, and promote the distribution of the test liquid to the entire surface of the coating rod 3.

[0031] Specifically, refer to Figure 4As shown, the pipette tip also includes a filter element 4, which is disposed within the reservoir 11. The filter element 4 is located at the end of the reservoir 11 furthest from the aspiration port 12. The filter element 4 is used to block the test liquid within the reservoir 11, and it allows airflow. Since the reservoir tube 1 needs to be tilted during the coating process, the filter element 4 prevents the test liquid from flowing towards the end away from the aspiration port 12, thus preventing the test liquid from entering the pipette body 100. It should be noted that the pipette body 100 is mainly used to drive the test liquid into and out of the reservoir tube 1; the pipette body 100 must not adhere to the test liquid to prevent different test liquids from mixing.

[0032] Specifically, the liquid storage tube 1, the connector 2, and the coating rod 3 are made of the same material, and the three can be configured as an integrated structure.

[0033] like Figure 5 As shown, the present invention also provides a pipette, including a pipette body 100 and a pipette tip. The pipette body 100 includes a housing 110 and a piston assembly 120 disposed on the housing 110, and a connector 130 disposed at the bottom of the housing 110. The end of the reservoir tube 1 in the pipette tip away from the aspiration port 12 is detachably connected to the connector 130. The connection between the reservoir tube 1 and the connector 130 can be a plug-in fixation or a threaded fixation. In this embodiment, the pipette body 100 is prior art, which draws the test liquid into the reservoir tube 1 or discharges the test liquid from the reservoir tube 1 by means of piston movement. The specific structure and working principle of the pipette body 100 will not be described in detail here.

[0034] like Figures 3 to 5 As shown, the present invention also provides a method for using a pipette to transfer the test solution in the original container to the culture medium. The method for using the pipette specifically includes the following steps: Step S1: Insert the pipette vertically into the original container, ensuring that the end of the reservoir tube 1 with the suction port 12 is immersed in the test solution. Position the applicator 3 above the surface of the test solution. Operate the pipette body 100 to draw the test solution into the reservoir tube 1. Specifically, during suction, place the pipette vertically so that the suction port 12 at the bottom of the reservoir tube 1 is submerged in the test solution. Simultaneously, control the insertion depth of the pipette tip in the test solution, ideally with the applicator 3 positioned above the surface of the test solution. Avoid contact between the applicator 3 and the test solution to prevent excessive test solution from adhering to its surface, thus preventing test solution dripping during transfer. Operate the piston assembly 120 to draw a certain volume of test solution into the reservoir tube 1.

[0035] Step S2: Drop the test solution onto the culture medium. Operate the piston assembly 120, using the piston movement of the piston assembly 120 to drive the test solution from the suction port 12 onto the culture medium.

[0036] Step S3: Adjust the pipette to an inclined position so that the spreading rod 3 is below the reservoir tube 1, allowing the spreading rod 3 to contact the culture medium. The operator pushes the spreading rod 3 back and forth along the axis of the reservoir tube 1 to spread the test solution evenly.

[0037] In some embodiments, when adding liquid to the culture medium, the pipette can be kept vertical, and the test solution can be dripped onto the culture medium through the aspiration port 12. Then, the pipette is tilted, and the test solution is spread evenly using the spreading rod 3.

[0038] In some embodiments, the dispensing and coating operations are performed simultaneously. After the pipette is moved above the culture medium, it is tilted. At this time, the steel ball 5 moves to the bypass channel 21 near the end of the coating rod 3. The pipette body 100 is operated, and part of the test solution is discharged from the aspiration port 12 and drips onto the culture medium. Another part of the test solution is discharged sequentially through the bypass through-hole 15, bypass channel 21, connecting hole 35, dispensing channel 33, and dispensing hole 34 to the outside of the coating rod 3. The test solution can flow onto the surface of the coating rod 3. Simultaneously, the operator pushes the coating rod 3 in a reciprocating motion, with dispensing and coating occurring simultaneously. This improves operational efficiency and facilitates rapid and uniform distribution of the test solution.

[0039] The beneficial effects of this embodiment are as follows: The pipette tip is used to draw a certain volume of test solution from the original container, then drops the test solution into the culture medium, and then spreads the test solution evenly. By setting a spreading rod 3 on the reservoir tube 1, pipetting and spreading are integrated together. After pipetting, the operator can use the spreading rod 3 to spread the test solution evenly without replacing the pipette tip. This shortens the operation time and improves the efficiency. Furthermore, it avoids contamination of the spreading rod 3 with the external environment during replacement operations, thus meeting the aseptic requirements of the workflow. Additionally, by setting a dispensing hole 34 on the spreading rod 3, the test solution can be discharged through the dispensing hole 34 and flow onto the surface of the spreading rod 3. During the spreading operation using the spreading rod 3, the test solution flows onto the surface of the spreading rod 3 through the dispensing hole 34, allowing for replenishment of the spreading area and keeping the spreading rod 3 moist, promoting even distribution of the test solution on the culture medium.

[0040] Although embodiments of the invention have been described above with reference to the accompanying drawings, the invention is not limited to the above embodiments, but can be made in various forms, and those skilled in the art will understand that the invention can be implemented in other specific forms without changing the technical spirit or essential characteristics of the invention. Therefore, it should be understood that the above embodiments are exemplary in all respects and not restrictive.

Claims

1. A pipette tip, characterized in that, The device includes a liquid storage tube, a connector, and a coating rod. The liquid storage tube has a liquid storage cavity, and one end of the liquid storage tube forms a suction port communicating with the liquid storage cavity. The connector is set at an angle to the liquid storage tube, with one end connected to the liquid storage tube near the suction port and the other end of the connector extending away from the suction port. The coating rod is connected to the end of the connector away from the liquid storage tube, and the length direction of the coating rod forms an angle with the axial direction of the liquid storage tube. The connector is provided with a bypass channel, the coating rod is provided with a plurality of liquid dispensing holes at intervals along its length, the side wall of the liquid storage tube is provided with a bypass through hole, the liquid storage chamber is connected to the bypass channel through the bypass through hole, the bypass channel is provided with a freely rolling steel ball, the steel ball is used to selectively block the bypass through hole, and all the liquid dispensing holes are connected to the bypass channel.

2. The pipette tip according to claim 1, characterized in that, The length direction of the coating rod is perpendicular to the axis of the liquid storage tube.

3. The pipette tip according to claim 1, characterized in that, Along the length of the coating rod, the connector is attached to the middle of the coating rod.

4. The pipette tip according to claim 1, characterized in that, Along the length of the coating rod, the coating rod includes a straight section and a curved section connected to each other. The straight section has a cylindrical structure, and the curved section is bent toward the side of the liquid storage tube at one end away from the straight section.

5. The pipette tip according to claim 4, characterized in that, The diameter of the curved section gradually decreases from the end near the straight section to the end away from the straight section.

6. The pipette tip according to claim 1, characterized in that, A liquid guiding groove is provided on the outer wall of the coating rod, and the liquid guiding groove is connected to the liquid dispensing hole.

7. The pipette tip according to claim 1, characterized in that, The side wall of the liquid storage tube is provided with a groove that fits the surface of the steel ball. The groove is connected to the bypass channel. The bypass through hole is located at the bottom of the groove. When the liquid storage tube is in a vertical state, the steel ball rolls into the groove and can block the bypass through hole.

8. The pipette tip according to any one of claims 1 to 7, characterized in that, A filter element is provided inside the liquid storage chamber. The filter element is located at the end of the liquid storage chamber away from the liquid suction port. The filter element is used to block the test liquid in the liquid storage chamber.

9. A pipette, characterized in that, It includes a pipette body and a pipette tip as described in any one of claims 1 to 8, wherein the reservoir tube in the pipette tip is detachably connected to the pipette body.

10. A method of using a pipette, characterized in that, The method of using the pipette as described in claim 9 to transfer the test solution from the original container to the culture medium specifically includes the following steps: Step S1: Insert the pipette vertically into the original container, and insert the end of the storage tube with the suction port into the test solution. Position the coating rod above the surface of the test solution, and operate the pipette body to draw the test solution into the storage tube. Step S2: Drop the test solution onto the culture medium; Step S3: Adjust the pipette to an inclined position so that the spreading rod contacts the culture medium, and use the spreading rod to spread the test solution evenly.

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