Highly decontaminating kitchen wet wipe and its preparation method

Abstract

This invention relates to the field of wet wipes technology, specifically disclosing a highly efficient stain-removing kitchen wet wipe and its preparation method. The wipe comprises a non-woven fabric layer and a stain-removing functional liquid impregnated within the non-woven fabric layer. The stain-removing functional liquid contains AES, cocamidopropyl betaine, lauryl betaine, a stain-removing active extract, mixed enzymes, glycerin, panthenol, and deionized water. The preparation method includes three steps: preparing the stain-removing active extract, formulating the stain-removing functional liquid, and impregnation molding. This invention optimizes the wetting and emulsifying properties of the system through a combination of multiple surfactants, combines mixed enzymes to decompose different types of kitchen stains, and incorporates plant fermentation extracts to assist cleaning and provide antibacterial effects. The addition of glycerin and panthenol alleviates the dryness caused by the cleaning ingredients. The overall formula is stable and effectively removes common kitchen grease and dried stains, with low skin irritation, making it suitable for various daily kitchen cleaning scenarios.

Description

Technical Field

[0001] This invention relates to the field of wet wipes technology, specifically to a highly effective stain-removing kitchen wet wipe and its preparation method. Background Technology

[0002] Kitchen wipes, as a type of disposable cleaning product, are widely used for daily cleaning in home kitchens, restaurants, and other places. They can be used to directly wipe away grease and food residue on surfaces such as stoves, range hoods, countertops, and the outer walls of tableware without the need for additional rinsing with water. They are convenient to use and can meet the requirements for quick cleaning, making them commonly used in daily kitchen and bathroom cleaning scenarios.

[0003] Existing kitchen wipes' functional liquid formulas mostly use conventional surfactants as the core cleaning component, combined with chemical bactericides to achieve antibacterial effects. The overall composition is relatively simple, and the cleaning effect relies solely on the emulsification effect of surfactants. The antibacterial performance depends entirely on additional antibacterial ingredients. Most products do not introduce biological enzymes and plant-derived active substances to participate in the cleaning process. The formula's action path is simple, and there is little development and compound application of plant-derived compound active ingredients.

[0004] Kitchen wipes with this type of formula have limited cleaning power when dealing with heavy oil stains and dried stains. They are difficult to quickly break down solidified grease and protein-based dirt, and often require repeated wiping to achieve the desired cleaning effect. At the same time, the antibacterial coverage of a single chemical antibacterial ingredient is relatively narrow. In addition, the high content of chemical surfactants is easy to leave residue on the wiping surface, which can irritate the user's skin. It is difficult to simultaneously achieve cleaning effect, antibacterial effect and gentleness. Summary of the Invention

[0005] To address the technical deficiencies in the background technology, this invention proposes a highly efficient stain-removing kitchen wipe and its preparation method, solving the aforementioned technical problems and meeting practical needs. The specific technical solution is as follows: A highly effective stain-removing kitchen wipe includes a non-woven fabric layer and a stain-removing functional liquid impregnated in the non-woven fabric layer; by weight, the stain-removing functional liquid comprises the following components: 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts laurylamide betaine, 5-10 parts stain-removing active extract, 1 part mixed enzyme, 2 parts glycerin, 1 part panthenol, and 100 parts deionized water.

[0006] As a further technical solution of the present invention, the mixed enzyme is composed of alkaline lipase, neutral protease and α-amylase in a mass ratio of 5:3:2; wherein the alkaline lipase activity is ≥10000U / g, the neutral protease activity is ≥5000U / g, and the α-amylase activity is ≥8000U / g.

[0007] As a further technical solution of the present invention, the cleaning active extract is a plant compound fermentation extract, and the fermentation raw materials of the plant compound fermentation extract contain three types of functional components: fermentation to produce essential oil raw materials, fermentation to produce saponin raw materials, and fermentation to produce plant amino acid raw materials. Among them, the fermented essential oil raw materials, fermented saponin raw materials, and fermented plant amino acid raw materials are all in dry powder form, and the weight ratio is (2-3):1:(1-2).

[0008] As a further technical solution of the present invention, the raw material for producing saponins through fermentation is selected from at least one of soapberry, soapberry pericarp, camellia seed cake, and horse chestnut. The fermented plant amino acid raw material is selected from at least one of soybean meal, peanut meal, and walnut meal; The fermented essential oil raw materials are selected from at least one of the following: citrus peel, lemon peel, grapefruit peel, bergamot peel, eucalyptus leaves, rosemary, and thyme.

[0009] A method for preparing highly effective stain-removing kitchen wipes includes the following steps: S1. Preparation of active decontamination extract: After mixing and pretreating the three types of fermentation raw materials, a fermentation base liquid is obtained. Fermentation bacteria are introduced into the fermentation base liquid for fermentation culture. After fermentation, the extract is subjected to coarse filtration, lysozyme enzymatic hydrolysis to break the cell wall, inactivation and fine filtration in sequence to obtain the active decontamination extract. S2. Preparation of cleaning function solution: Add AES, cocamidopropyl betaine, lauryl betaine, cleaning active extract, mixed enzyme, glycerin and panthenol to deionized water according to the weight ratio, and stir until completely dissolved and mixed to obtain the cleaning function solution. S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning liquid. After it is fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

[0010] As a further technical solution of the present invention, in step S1, the specific operation of raw material pretreatment is as follows: the three types of functional component fermentation raw materials are mixed according to the ratio and then crushed to 40 mesh, sterilized by irradiation with a dose of 4-8 kGy, and then sterile deionized water is added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain fermentation base liquid.

[0011] As a further technical solution of the present invention, in step S1, the fermentation strains are Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid, and the inoculation amount is 1-3 v / v; the fermentation culture conditions are: fermentation temperature 39-41℃, shaking speed 100-200 r / min, and total fermentation time 36-48 h.

[0012] As a further technical solution of the present invention, in step S1, the amount of lysozyme added is 0.6-1% of the total mass of the fermentation broth, the enzymatic hydrolysis temperature is controlled at 35-40℃, and the enzymatic hydrolysis is carried out by low-speed stirring for 1-3 hours; after the enzymatic hydrolysis is completed, the temperature is raised to 85℃ and kept at 15 minutes to inactivate the enzyme and residual live bacteria, and after cooling, it is filtered through a 0.45μm microporous membrane to obtain the decontamination active extract.

[0013] The beneficial effects of this invention are as follows: This kitchen wipe uses a three-surfactant compound system, combined with saponins from the cleaning active extract, to reduce the interfacial tension of oil stains. It also features a mixed enzyme system of alkaline lipase, neutral protease, and α-amylase to break down common kitchen stains such as grease, protein, and starch, enhancing the wipe's cleaning effect on heavy oil stains. The cleaning active extract is produced through multi-strain fermentation; the resulting plant essential oils mask the raw material odor of the cleaning solution, improving the user experience. The extract itself has antibacterial properties, inhibiting Escherichia coli and Staphylococcus aureus, improving the wipe's hygienic performance during use. The formula includes glycerin and panthenol, along with plant amino acids from fermentation, reducing skin irritation from the cleaning ingredients, balancing cleaning power with gentleness, making it suitable for daily kitchen cleaning. Detailed Implementation

[0014] The embodiments of the present invention will be described below with reference to relevant examples. The embodiments of the present invention are not limited to those described below, and the present invention relates to necessary knowledge in this technical field. It should be considered as well-known technology in this technical field, and is known and mastered by those skilled in the art. Experimental methods in the following examples that do not specify specific conditions are generally performed under conventional conditions in the art or according to the manufacturer's recommendations. Unless otherwise specified, the raw materials and reagents used are all commercially available from the conventional market. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention are within the scope of protection claimed by the present invention.

[0015] A highly effective stain-removing kitchen wipe includes a non-woven fabric layer and a stain-removing functional liquid impregnated in the non-woven fabric layer; by weight, the stain-removing functional liquid comprises the following components: 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts laurylamide betaine, 5-10 parts stain-removing active extract, 1 part mixed enzyme, 2 parts glycerin, 1 part panthenol, and 100 parts deionized water.

[0016] In this invention's formulation, AES serves as the primary cleaning ingredient, combined with cocamidopropyl betaine and lauryl betaine. This combination optimizes the wetting and emulsifying properties of the system, reducing interfacial tension when applied to oily surfaces, thus loosening the oil and making it easier to wipe away. The active cleaning extract contains saponins, which enhance the cleaning effect. It also possesses antibacterial properties, inhibiting common bacteria such as Escherichia coli and Staphylococcus aureus. The mixed enzyme, composed of lipase, protease, and amylase, decomposes grease, protein residues, and starchy stains in the kitchen, respectively. Working in conjunction with surfactants, it improves the removal of hardened, dried grime.

[0017] Glycerin and panthenol, as skincare ingredients, act on the skin surface during cleansing, alleviating the dryness and degreasing sensation caused by cleansing ingredients. Combined with plant amino acids fermented in the active cleansing extract, they further reduce skin irritation and maintain hand moisture. The active cleansing extract contains fermented plant essential oils, which adjust the product's scent, mask any unpleasant odors from the raw materials, and improve the user experience. The overall formula uses deionized water as a solvent; after thorough dissolution and mixing of all components, it is loaded onto a non-woven fabric layer, ensuring stable release of the active ingredients during use. Suitable for various everyday kitchen cleaning scenarios.

[0018] As one of the preferred embodiments of the present invention, the mixed enzyme is composed of alkaline lipase, neutral protease and α-amylase in a mass ratio of 5:3:2; wherein the alkaline lipase activity is ≥10000U / g, the neutral protease activity is ≥5000U / g, and the α-amylase activity is ≥8000U / g.

[0019] Specifically, the mixed enzyme is composed of alkaline lipase, neutral protease, and α-amylase in a mass ratio of 5:3:2. Since grease is the most common type of everyday kitchen stain, the proportion of alkaline lipase is increased accordingly. Neutral protease is used to break down protein residues such as egg and meat stains, while α-amylase is used to break down starch stains such as rice and flour stains. The three enzymes target different types of stain components. Minimum enzyme activity requirements are set for each component to ensure the decomposition efficiency per unit mass of the mixed enzyme and maintain the stability of the formula's cleaning effect. Alkaline lipase can function in a weakly alkaline environment, making it suitable for the acidic or alkaline environment of the surfactant system in this formula. Neutral protease operates under gentle conditions, reducing skin irritation. The combination of these three enzymes can cover various organic stains in the kitchen. For dried, complex stains, it works synergistically with the surfactants and plant saponins in the formula to enhance stain removal and dispersion, achieving its corresponding effect even at relatively low dosages.

[0020] As one of the preferred embodiments of the present invention, the cleaning active extract is a plant compound fermentation extract, and the fermentation raw materials of the plant compound fermentation extract contain three types of functional components: fermentation to produce essential oil raw materials, fermentation to produce saponin raw materials, and fermentation to produce plant amino acid raw materials. Among them, the fermented essential oil raw materials, fermented saponin raw materials, and fermented plant amino acid raw materials are all in dry powder form, and the weight ratio is (2-3):1:(1-2).

[0021] Specifically, the cleaning active extract is a plant-based compound fermentation extract. The fermentation raw materials are divided into three categories based on the core functional substances produced after fermentation, corresponding to the functions of producing essential oils, saponins, and plant amino acids, respectively. The three types of raw materials are mixed and fermented simultaneously, generating multiple active substances within the same system, eliminating the need for separate extraction and compounding, thus simplifying the preparation process. Microbial fermentation decomposes the cell wall structure of the plant raw materials, increasing the dissolution rate of various active ingredients and ensuring the concentration of effective components in the extract.

[0022] Fermentation-derived saponin raw materials can be selected from at least one of the following: soapberry, soapberry peel, camellia seed cake, and horse chestnut. These raw materials themselves contain natural saponin components, which are fully released into the liquid phase after fermentation, thus helping to enhance the system's cleaning ability and also possessing a certain antibacterial effect. Fermentation-derived plant amino acid raw materials can be selected from at least one of the following: soybean meal, peanut meal, and walnut meal. These raw materials are mostly by-products of oilseed processing, rich in plant protein, and after microbial degradation, they generate small-molecule plant amino acids, which can alleviate the skin irritation caused by cleansing ingredients.

[0023] For fermentation to produce essential oils, at least one of the following raw materials can be selected: citrus peel, lemon peel, grapefruit peel, bergamot peel, eucalyptus leaves, rosemary, and thyme. During fermentation, the aromatic essential oils within the plant tissues are gradually released, which can regulate the odor of the extract and mask any unpleasant smells from the raw materials themselves. The essential oil components of some aromatic raw materials can also help enhance antibacterial effects. Various raw materials can be used individually or in combination according to the actual formula; adjusting the ratio can change the proportion of various active substances in the extract to suit different product positioning.

[0024] A method for preparing highly effective stain-removing kitchen wipes includes the following steps: S1. Preparation of active decontamination extract: After mixing and pretreating the three types of fermentation raw materials, a fermentation base liquid is obtained. Fermentation bacteria are introduced into the fermentation base liquid for fermentation culture. After fermentation, the extract is subjected to coarse filtration, lysozyme enzymatic hydrolysis to break the cell wall, inactivation and fine filtration in sequence to obtain the active decontamination extract. S2. Preparation of cleaning function solution: Add AES, cocamidopropyl betaine, lauryl betaine, cleaning active extract, mixed enzyme, glycerin and panthenol to deionized water according to the weight ratio, and stir until completely dissolved and mixed to obtain the cleaning function solution. S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning liquid. After it is fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

[0025] Specifically, in step S1, when preparing the decontamination active extract, the three types of functional fermentation raw materials are first mixed and pretreated, then inoculated with the corresponding fermentation strains for constant temperature culture, allowing the microorganisms to metabolize and produce various active substances. After fermentation, most of the solid residue is removed by coarse filtration, then lysozyme is added for enzymatic hydrolysis to break the cell walls and release the active ingredients within the bacterial cells. After enzymatic hydrolysis, the temperature is raised to complete the inactivation treatment, removing residual live bacteria and enzyme activity. Finally, a clear extract is obtained by fine filtration, which can effectively retain the saponins, plant amino acids, and essential oil components, while removing impurities and improving the stability of the system.

[0026] In step S2, the surfactant, detergency active extract, mixed enzyme and skin care components are added to deionized water in sequence according to the weight ratio. Stir at room temperature until all components are completely dissolved and mixed evenly. Control the stirring rate during the process to avoid generating too much foam and ensure the uniformity of the functional liquid system. In step S3, during the third step of impregnation molding, the non-woven fabric substrate is completely immersed in the detergency functional liquid. After being fully soaked, it is taken out, cut according to specifications, and then sealed and packaged to obtain the finished wet wipes.

[0027] As one of the preferred embodiments of the present invention, in step S1, the specific operation of raw material pretreatment is as follows: the three types of functional component fermentation raw materials are mixed according to the ratio and then crushed to 40 mesh, sterilized by irradiation with a dose of 4-8 kGy, and then sterile deionized water is added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain fermentation base liquid.

[0028] Specifically, the three types of functional fermentation raw materials are mixed according to the specified ratio and then pulverized to 40 mesh. This facilitates the full effect of subsequent sterilization and improves the contact efficiency between microorganisms and raw materials during fermentation, promoting the dissolution of internal active ingredients. Irradiation sterilization using a dose of 4-8 kGy can kill miscellaneous bacteria on the surface and inside of the raw materials, avoiding contamination during subsequent fermentation. Furthermore, irradiation sterilization is carried out at room temperature, which will not damage the heat-sensitive plant components in the raw materials, thus preserving more of the raw materials' own active substances. After sterilization, the mixture is stirred evenly with sterile deionized water at a solid-liquid ratio of 1:20 (g / mL) to form a homogeneous fermentation base liquid. This provides a stable liquid environment for the subsequent inoculation of fermentation bacteria, facilitating uniform dispersion and growth after inoculation.

[0029] As one of the preferred embodiments of the present invention, in step S1, the fermentation strains are Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid, and the inoculation amount is 1-3 v / v; the fermentation culture conditions are: fermentation temperature 39-41℃, shaking speed 100-200 r / min, and total fermentation time 36-48 h.

[0030] Specifically, the fermentation stage employs co-inoculation with Bacillus amyloliquefaciens seed culture, Lactobacillus plantarum seed culture, and Bacillus subtilis seed culture. These cultures can respectively decompose the starch, protein, and cellulose components in the raw materials, promoting the release of active substances such as saponins, essential oils, and amino acids from plant cells, while simultaneously generating small-molecule active products. The inoculation amounts of all three strains are controlled at 1-3 v / v%, ensuring that the initial bacterial concentration of the fermentation system is within a reasonable range, shortening the fermentation start-up cycle, and avoiding waste of microorganisms due to excessive inoculation.

[0031] The fermentation temperature was set at 39-41℃, which falls within the optimal growth range for the three microbial strains, ensuring stable metabolic activity. The shaking speed was controlled at 100-200 rpm to provide sufficient dissolved oxygen for the aerobic bacteria and ensure uniform mixing of the materials within the fermentation system. The total fermentation time was set at 36-48 hours to guarantee thorough decomposition of the raw materials and complete dissolution of active ingredients, while preventing excessive fermentation time that could lead to the proliferation of unwanted microorganisms or degradation of active ingredients.

[0032] As one of the preferred embodiments of the present invention, in step S1, the amount of lysozyme added is 0.6-1% of the total mass of the fermentation broth, the enzymatic hydrolysis temperature is controlled at 35-40℃, and the enzymatic hydrolysis is carried out by low-speed stirring for 1-3 hours; after the enzymatic hydrolysis is completed, the temperature is raised to 85℃ and kept at 15 minutes to inactivate the enzyme and residual live bacteria, and after cooling, it is filtered through a 0.45μm microporous membrane to obtain a cleaned active extract.

[0033] After coarse filtration following fermentation, this step involves adding 0.6-1% lysozyme for enzymatic hydrolysis. Hydrolysis is performed at 35-40℃ with low-speed stirring for 1-3 hours. This decomposes the cell wall structure of the bacteria in the fermentation system, releasing intracellular active metabolites and increasing the content of effective substances in the extract. After hydrolysis, the system is heated to 85℃ and held for 15 minutes to inactivate the lysozyme and kill any remaining live bacteria, terminating the fermentation process and preventing spoilage during subsequent storage. The treated liquid is then cooled and filtered through a 0.45μm microporous membrane to remove residual bacterial fragments and fine solid impurities, resulting in a clear, decontaminated, and active extract. This improves the homogeneity of the extract and prevents precipitation during subsequent preparation of functional solutions.

[0034] The present invention will be further described below through examples and comparative examples. Example 1

[0035] S1. Preparation of Decontamination Active Extract: The fermentation raw materials were mixed in a 2:1:1 mass ratio according to the following proportions: saponin-producing (a mixture of soapberry and soapberry peel at a mass ratio of 1:1), plant amino acid-producing (soybean meal), and essential oil-producing (a mixture of citrus peel and lemon peel at a mass ratio of 1:1). The mixture was pulverized to 40 mesh and sterilized by irradiation at a dose of 6 kGy. Then, sterile deionized water was added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain the fermentation base liquid. Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid were inoculated into the fermentation base liquid at an inoculation rate of 2 v / v%, and fermented at 40℃ and a shaking speed of 150 r / min for 42 h. After fermentation, coarse filtration is performed first, and 0.8% of the total mass of lysozyme is added to the filtrate. The mixture is stirred at low speed at 37℃ for 2 hours for enzymatic hydrolysis. Then, the temperature is raised to 85℃ and kept at 15 minutes for inactivation. After cooling, the mixture is filtered through a 0.45μm microporous membrane for fine filtration. The clear filtrate is then taken to obtain the decontamination active extract.

[0036] S2. Preparation of the detergency solution: By weight, add 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts laurylamide betaine, 5 parts the detergency active extract obtained in S1, 1 part mixed enzyme, 2 parts glycerol, and 1 part panthenol to 100 parts deionized water, and stir until completely dissolved and mixed. The mixed enzyme is composed of alkaline lipase, neutral protease, and α-amylase in a mass ratio of 5:3:2, wherein the alkaline lipase activity is ≥10000 U / g, the neutral protease activity is ≥5000 U / g, and the α-amylase activity is ≥8000 U / g.

[0037] S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning functional liquid obtained in S2. After being fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

[0038] Example 2

[0039] S1. Preparation of the active extract for decontamination: The fermentation raw materials were mixed in a 2:1:2 mass ratio according to the following proportions: saponin-producing (tea oil cake and horse chestnut in a 1:1 mass ratio), plant amino acid-producing (peanut meal and walnut meal in a 1:1 mass ratio), and essential oil-producing (grapefruit peel, eucalyptus leaves, and rosemary in a 1:1:1 mass ratio). The mixture was pulverized to 40 mesh and sterilized by irradiation at a dose of 6 kGy. Then, sterile deionized water was added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain the fermentation base liquid. Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid were inoculated into the fermentation base liquid at an inoculation rate of 2 v / v%, and fermented at 40℃ and a shaking speed of 150 r / min for 42 h. After fermentation, coarse filtration is performed first, and 0.8% of the total mass of lysozyme is added to the filtrate. The mixture is stirred at low speed at 37℃ for 2 hours for enzymatic hydrolysis. Then, the temperature is raised to 85℃ and kept at 15 minutes for inactivation. After cooling, the mixture is filtered through a 0.45μm microporous membrane for fine filtration. The clear filtrate is then taken to obtain the decontamination active extract.

[0040] S2. Preparation of the detergency solution: By weight, add 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts laurylamide betaine, 8 parts the detergency active extract obtained in S1, 1 part mixed enzyme, 2 parts glycerol, and 1 part panthenol to 100 parts deionized water, and stir until completely dissolved and mixed. The mixed enzyme is composed of alkaline lipase, neutral protease, and α-amylase in a mass ratio of 5:3:2, wherein the alkaline lipase activity is ≥10000 U / g, the neutral protease activity is ≥5000 U / g, and the α-amylase activity is ≥8000 U / g.

[0041] S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning functional liquid obtained in S2. After being fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

[0042] Example 3

[0043] S1. Preparation of Decontamination Active Extract: The fermentation raw materials were mixed in a 3:1:1 mass ratio according to the following proportions: saponin-producing (Sapindus mukorossi peel and Camellia oleifera seed oil mixed at a mass ratio of 1:1), plant amino acid-producing (soybean meal), and essential oil-producing (lemon peel, bergamot peel, and thyme mixed at a mass ratio of 2:1:1). The mixture was pulverized to 40 mesh and sterilized by irradiation at a dose of 6 kGy. Then, sterile deionized water was added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain the fermentation base liquid. Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid were inoculated into the fermentation base liquid at an inoculation rate of 2 v / v%, and fermented at 40℃ and a shaking speed of 150 r / min for 42 h. After fermentation, coarse filtration is performed first, and 0.8% of the total mass of lysozyme is added to the filtrate. The mixture is stirred at low speed at 37℃ for 2 hours for enzymatic hydrolysis. Then, the temperature is raised to 85℃ and kept at 15 minutes for inactivation. After cooling, the mixture is filtered through a 0.45μm microporous membrane for fine filtration. The clear filtrate is then taken to obtain the decontamination active extract.

[0044] S2. Preparation of the detergency solution: By weight, add 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts laurylamide betaine, 10 parts the detergency active extract obtained in S1, 1 part mixed enzyme, 2 parts glycerol, and 1 part panthenol to 100 parts deionized water, and stir until completely dissolved and mixed. The mixed enzyme is composed of alkaline lipase, neutral protease, and α-amylase in a mass ratio of 5:3:2, wherein the alkaline lipase activity is ≥10000 U / g, the neutral protease activity is ≥5000 U / g, and the α-amylase activity is ≥8000 U / g.

[0045] S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning functional liquid obtained in S2. After being fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

[0046] Comparative Example 1 The difference between Comparative Example 1 and Example 2 is that in the formula of the detergency liquid in S2, the detergency active extract is omitted, and 8 parts of the detergency active extract are replaced with an equal amount of deionized water. The remaining components and preparation process are the same as in Example 2.

[0047] Comparative Example 2 The difference between Comparative Example 2 and Example 2 is that in the formula of the cleaning functional liquid in S2, the mixed enzyme is omitted and 1 part of the mixed enzyme is replaced with an equal amount of deionized water. The remaining components and preparation process are the same as in Example 2.

[0048] Comparative Example 3 The difference between Comparative Example 3 and Example 2 is that in the stain-removing liquid formula of S2, 8 parts of AES, 4 parts of cocamidopropyl betaine, and 4 parts of laurylamide betaine are replaced with 16 parts of AES alone, while the remaining components and preparation process are the same as in Example 2.

[0049] Comparative Example 4 The difference between Comparative Example 4 and Example 2 is as follows: In S1, after fermentation, the active extract for cleaning was coarsely filtered, the lysozyme enzymatic hydrolysis and cell wall breaking step was omitted, and the temperature was directly raised to 85°C and kept at 15 min for inactivation. After cooling, it was finely filtered through a 0.45 μm microporous membrane. The resulting filtrate was used as the active extract for cleaning to prepare the functional cleaning solution. The remaining steps and formulas were the same as in Example 2.

[0050] Comparative Example 5 The difference between Comparative Example 5 and Example 2 is as follows: When preparing the active cleaning extract in S1, fermentation and enzymatic hydrolysis processes are not used. Instead, the same type and proportion of fermentation raw materials as in Example 2 are mixed and pulverized to 40 mesh, and deionized water is added at a solid-liquid ratio of 1:20. The mixture is stirred and extracted at 90°C for 2 hours, filtered, and concentrated to the same solid content as the active cleaning extract in Example 2. This extract is used to prepare the cleaning functional liquid. The remaining steps and formulas are the same as in Example 2.

[0051] Comparative Example 6 The difference between Comparative Example 6 and Example 2 is that in the stain-removing functional liquid formula of S2, 1 part of mixed enzyme (alkaline lipase: neutral protease: α-amylase = 5:3:2) was replaced with an equal amount of single alkaline lipase (enzyme activity ≥10000U / g), and the remaining components and preparation process were the same as in Example 2.

[0052] Performance testing Oil and stain removal performance: tested in accordance with GB_T27728.1-2024 "Wet wipes and similar products - Part 1: General requirements"; Antibacterial rate: The test strains were Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Test method: A small amount of bacterial cells was placed in 10 ml of sterile water and serially diluted to prepare a 10:10 concentration. 7 Prepare a bacterial suspension at CFU / mL for later use. Dilute the detergent samples from Examples 1-3 and Comparative Examples 1-6 with deionized water at a mass ratio of 1:4 to obtain diluted solutions. Take 0.5 mL of the bacterial suspension, add 5 mL of the diluted solution, and incubate at 37°C for 10 min. Add 4.5 mL of distilled water to the blank control group. Use the dilution-poured plate method to test the bactericidal effect of the detergents from the examples and comparative examples against Escherichia coli and Staphylococcus aureus.

[0053]

[0054] The comparative test results show that, in Comparative Example 1, the oil removal rate decreased from 94.2% in Example 2 to 85.1% after the absence of the active cleaning extract; in Comparative Example 2, the oil removal rate further decreased to 78.9% without the addition of mixed enzymes, the lowest value among all test groups. In Comparative Example 3, the oil removal rate decreased to 88.7% after replacing the compound surfactant with a single AES, indicating that the addition of cocamidopropyl betaine and lauryl betaine has a practical effect on improving the oil removal effect. The preparation process also affects the cleaning performance. In Comparative Example 4, the oil removal rate decreased to 90.5% after omitting the lysozyme hydrolysis step; in Comparative Example 5, the oil removal rate was only 83.6% after using hot water extraction instead of fermentation, reflecting that microbial fermentation and enzymatic cell wall breaking treatment help release the active cleaning substances in the raw materials. In Comparative Example 6, the oil removal rate decreased to 87.2% after replacing the compound enzyme with a single lipase, indicating that for mixed stains composed of grease, protein, and starch in the kitchen, the combination of lipase, protease, and α-amylase is more efficient than a single enzyme in decomposing the stains.

[0055] Antibacterial test data showed that the inhibitory effect of the formulation on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa mainly came from the detergent-active extract. In Comparative Example 1, omitting the extract reduced the inhibition rates of the three bacteria from 98.5%, 96.8%, and 95.5% to 65.3%, 60.2%, and 55.8%, respectively. Comparative Examples 2, 3, and 6, which changed the type of enzyme or surfactant composition, showed no significant difference in inhibition rates compared to Example 2, indicating that these components themselves do not contribute to antibacterial activity. Comparative Example 4, which did not undergo lysozyme cell disruption, showed a decrease in inhibition rate compared to Example 2, indicating that intracellular metabolites also contain some antibacterial activity. The antibacterial rate of the aqueous extract in Comparative Example 5 was only slightly higher than that of Comparative Example 1, which did not contain any extract, indicating that conventional water extraction cannot effectively dissolve saponins and plant essential oils in the raw materials. Therefore, preparing the detergent-active extract using a fermentation-enzymatic hydrolysis method is a necessary condition to ensure stable antibacterial properties in the product.

[0056] The above description is only a preferred embodiment of the present invention. It should be noted that those skilled in the art can make several improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A highly effective stain-removing kitchen wipe, characterized in that, Includes a non-woven fabric layer and a cleaning liquid impregnated in the non-woven fabric layer; By weight, the cleaning functional liquid comprises the following components: 8 parts AES, 4 parts cocamidopropyl betaine, 4 parts lauroamide betaine, 5-10 parts cleaning active extract, 1 part mixed enzyme, 2 parts glycerol, 1 part panthenol, and 100 parts deionized water.

2. The kitchen wipes with high efficiency in removing stains according to claim 1, characterized in that, The mixed enzyme is composed of alkaline lipase, neutral protease and α-amylase in a mass ratio of 5:3:2; wherein the alkaline lipase activity is ≥10000U / g, the neutral protease activity is ≥5000U / g, and the α-amylase activity is ≥8000U / g.

3. The kitchen wipes with high efficiency in removing stains according to claim 1, characterized in that, The cleaning active extract is a plant compound fermentation extract, and the fermentation raw materials of the plant compound fermentation extract contain three types of functional components: fermentation to produce essential oil raw materials, fermentation to produce saponin raw materials, and fermentation to produce plant amino acid raw materials. Among them, the fermented essential oil raw materials, fermented saponin raw materials, and fermented plant amino acid raw materials are all in dry powder form, and the weight ratio is (2-3):1:(1-2).

4. The highly efficient stain-removing kitchen wipes according to claim 3, characterized in that, The saponin-producing raw materials for fermentation are selected from at least one of soapberry, soapberry pericarp, camellia seed cake, and horse chestnut. The fermented plant amino acid raw material is selected from at least one of soybean meal, peanut meal, and walnut meal; The fermented essential oil raw materials are selected from at least one of the following: citrus peel, lemon peel, grapefruit peel, bergamot peel, eucalyptus leaves, rosemary, and thyme.

5. The method for preparing the highly efficient stain-removing kitchen wipes according to any one of claims 1 to 4, characterized in that, Includes the following steps: S1. Preparation of active decontamination extract: After mixing and pretreating the three types of fermentation raw materials, a fermentation base liquid is obtained. Fermentation bacteria are introduced into the fermentation base liquid for fermentation culture. After fermentation, the extract is subjected to coarse filtration, lysozyme enzymatic hydrolysis to break the cell wall, inactivation and fine filtration in sequence to obtain the active decontamination extract. S2. Preparation of cleaning function solution: Add AES, cocamidopropyl betaine, lauryl betaine, cleaning active extract, mixed enzyme, glycerin and panthenol to deionized water according to the weight ratio, and stir until completely dissolved and mixed to obtain the cleaning function solution. S3. Impregnation molding: The non-woven fabric substrate is immersed in the cleaning liquid. After it is fully soaked, it is taken out, cut, sealed and packaged to obtain the finished kitchen wipes.

6. The method for preparing the highly efficient stain-removing kitchen wipes according to claim 5, characterized in that, In step S1, the specific operation of raw material pretreatment is as follows: the three types of functional component fermentation raw materials are mixed according to the ratio and then crushed to 40 mesh. They are then sterilized by irradiation with a dose of 4-8 kGy. Subsequently, sterile deionized water is added at a solid-liquid ratio of 1:20 (g / mL) and stirred evenly to obtain fermentation base liquid.

7. The method for preparing the highly efficient stain-removing kitchen wipes according to claim 5, characterized in that, In step S1, the fermentation strains are Bacillus amyloliquefaciens seed liquid, Lactobacillus plantarum seed liquid, and Bacillus subtilis seed liquid, with an inoculation amount of 1-3 v / v; the fermentation conditions are: fermentation temperature 39-41℃, shaking speed 100-200 r / min, and total fermentation time 36-48 h.

8. The method for preparing the highly efficient stain-removing kitchen wipes according to claim 5, characterized in that, In step S1, the amount of lysozyme added is 0.6-1% of the total mass of the fermentation broth, the enzymatic hydrolysis temperature is controlled at 35-40℃, and the enzymatic hydrolysis is carried out by low-speed stirring for 1-3 hours. After the enzymatic hydrolysis is completed, the temperature is raised to 85℃ and kept at 15 minutes to inactivate the enzyme and residual live bacteria. After cooling, the solution is filtered through a 0.45μm microporous membrane, and the clear filtrate is taken to obtain the decontamination active extract.

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