Cosmetic composition for improving skin containing nicotinamide adenine dinucleotide

Abstract

The present invention relates to a cosmetic composition containing nicotinamide adenine dinucleotide for improving skin, and more particularly, to a cosmetic composition having improved intracellular potency of nicotinamide adenine dinucleotide, which is capable of increasing the concentration of nicotinamide adenine dinucleotide delivered into skin cells, and providing effects of inhibiting skin aging, improving skin quality, improving wrinkles and elasticity, skin regeneration, and strengthening skin barrier.

Description

Technical Field

[0001] This invention relates to a cosmetic composition for improving skin containing nicotinamide adenine dinucleotide, and more specifically, to a cosmetic composition comprising a combination of nicotinamide adenine dinucleotide, glycyrrhetinic acid and quercetin, which can increase the amount of nicotinamide adenine dinucleotide delivered to skin cells and provide an anti-aging effect. Background Technology

[0002] Nicotinamide adenine dinucleotide is biochemically very unstable. Therefore, in order to experience skin-improving effects, it must be formulated in excess in cosmetics; however, even so, its effects are still limited due to homeostasis mechanisms.

[0003] As an alternative to address the intracellular potency issue of nicotinamide adenine dinucleotide, there is a derivative of nicotinamide adenine dinucleotide, namely nicotinamide adenine dinucleotide phosphate (NADP). However, it is unclear whether this derivative has the same sufficient inhibitory effect on skin aging as nicotinamide adenine dinucleotide.

[0004] Against this backdrop, in order to solve the problem of intracellular potency of nicotinamide adenine dinucleotide, the inventors prepared a cosmetic composition using a combination of nicotinamide adenine dinucleotide, glycyrrhetinic acid, and quercetin. The results confirmed that the composition could solve the existing potency problem and ensure stability. When applied to the skin, the amount of nicotinamide adenine dinucleotide delivered to skin cells was not reduced by various mechanisms, and it provided an anti-aging effect on the skin, thus completing the present invention. Summary of the Invention

[0005] Technical issues

[0006] The present invention aims to provide a cosmetic composition that enhances the potency of exogenous nicotinamide adenine dinucleotide.

[0007] Problem-solving methods

[0008] This will be explained in detail below. Furthermore, the descriptions and embodiments disclosed in this invention are also applicable to other descriptions and embodiments. That is, all combinations of the various elements disclosed in this invention fall within the scope of this invention. Moreover, the scope of this invention should not be construed as being limited to the specific descriptions below.

[0009] To achieve the above objectives, a first aspect of the present invention provides a cosmetic composition comprising: nicotinamide adenine dinucleotide; glycyrrhetinic acid; and quercetin.

[0010] To achieve the above objectives, a second aspect of the present invention provides a cosmetic composition comprising: nicotinamide adenine dinucleotide; glycyrrhetinic acid; quercetin; and sitosterol or tocopherol acetate.

[0011] The present invention will now be described in more detail.

[0012] The term "Nicotinamide adenine dinucleotide (NAD+)" in this invention refers to a substance derived from nicotinamide, a form of vitamin B3, which functions as an electron transporter in intracellular biochemical reactions such as redox reactions. Furthermore, it plays a crucial role in preventing skin aging, including skin cell energy production, stress relief, DNA repair, and inflammation regulation. With age, the concentration of NAD+ within skin cells naturally declines, leading to a weakening of the skin's natural regenerative capacity and accelerated aging. Therefore, methods to maintain or increase NAD+ levels within skin cells are being investigated as an important strategy for mitigating skin aging.

[0013] As mentioned above, due to the highly unstable biochemical properties of NAD+, instances of directly incorporating it into cosmetic compositions are extremely rare. Furthermore, NAD+ rapidly transforms into an inactive form within cells, thus making it difficult to expect sufficient anti-aging and wrinkle-improving effects when applied to the skin.

[0014] Therefore, the present invention aims to solve the aforementioned problem of difficulty in maintaining high concentrations and high biological activity in cells when applied to the skin by using glycyrrhetinic acid, quercetin, and sitosterol or tocopheryl acetate together with NAD+.

[0015] The term "glycyrrhetinic acid" in this invention refers to one of the main active ingredients of licorice, which possesses skin-soothing, anti-inflammatory, antioxidant, and antibacterial properties. It is also included in cosmetic compositions because of its ability to reduce skin irritation and protect the skin from external stimuli to maintain a healthy skin condition.

[0016] The nicotinamide adenine dinucleotide and glycyrrhetinic acid may be contained in weight ratios of 1:0.0004 to 1:0.4, 1:0.0004 to 1:0.3, 1:0.0004 to 1:0.25, 1:0.004 to 1:0.4, 1:0.004 to 1:0.3, 1:0.004 to 1:0.25, 1:0.01 to 1:0.4, 1:0.01 to 1:0.3, or 1:0.01 to 1:0.25.

[0017] The term "quercetin" in this invention refers to a flavonoid polyphenol compound that is naturally found in various plants, including fruits, vegetables, leaves, seeds, and grains. As a potent antioxidant, it is known to possess a wide range of physiological activities, including anti-inflammatory, antibacterial, anti-allergic, and anti-cancer effects. It is particularly effective in preventing skin aging, repairing damaged skin, and relieving irritation and inflammation.

[0018] The nicotinamide adenine dinucleotide and quercetin may be contained in weight ratios of 1:0.0004 to 1:0.4, 1:0.0004 to 1:0.3, 1:0.0004 to 1:0.25, 1:0.004 to 1:0.4, 1:0.004 to 1:0.3, 1:0.004 to 1:0.25, 1:0.01 to 1:0.4, 1:0.01 to 1:0.3, or 1:0.01 to 1:0.25.

[0019] The term "sitosterol" in this invention refers to a type of plant sterol with a steroidal structure and is a naturally occurring substance. It is primarily extracted from plant oils such as soybeans, wheat, corn, peanuts, and sesame. In particular, it protects and soothes the skin and provides repair effects for damaged skin through its moisturizing, anti-inflammatory, antioxidant, and emulsifying stabilizing functions.

[0020] The nicotinamide adenine dinucleotide and sitosterol may be contained in weight ratios of 1:0.0004 to 1:0.4, 1:0.0004 to 1:0.3, 1:0.0004 to 1:0.25, 1:0.004 to 1:0.4, 1:0.004 to 1:0.3, 1:0.004 to 1:0.25, 1:0.01 to 1:0.4, 1:0.01 to 1:0.3, or 1:0.01 to 1:0.25.

[0021] The term "tocopheryl acetate" in this invention refers to a vitamin E derivative, a synthetic compound bound to acetic acid. Vitamin E (tocopherol), known as a potent antioxidant, is sensitive to light and heat and readily decomposes in cosmetics; however, tocopheryl acetate is more stable under such conditions. Tocopheryl acetate provides antioxidant, skin-protective, regenerative, moisturizing, and skin-improving effects.

[0022] The nicotinamide adenine dinucleotide and tocopherol acetate may be contained in weight ratios of 1:0.0004 to 1:1, 1:0.0004 to 1:0.8, 1:0.0004 to 1:0.5, 1:0.004 to 1:1, 1:0.004 to 1:0.8, 1:0.004 to 1:0.5, 1:0.01 to 1:1, 1:0.01 to 1:0.8, or 1:0.01 to 1:0.5.

[0023] The biological mechanisms by which intracellular NAD+ activation is associated with increased cell lifespan and longevity are known (Laurent Mouchiroud, Cell, 2013). A representative example is that the activation of the silencing regulatory protein sirtuin requires NAD+, and studies have shown that increasing intracellular NAD+ can achieve anti-aging effects.

[0024] However, when NAD+ is injected into skin cells from the outside, it is rapidly broken down due to its own homeostasis mechanism. The most representative mechanism is the breakdown of NAD+ by the CD38 protein, also known as NADase (NAD+ degrading enzyme). Studies have shown that inhibiting the activity or expression of CD38 can increase the intracellular NAD+ concentration. (Carlos Escande, Diabetes. 2013)

[0025] In this invention, it was confirmed that CD38 is overexpressed when skin cells are treated with NAD+, and that quercetin, glycyrrhetinic acid, sitosterol, tocopheryl acetate, and combinations thereof can effectively inhibit this CD38 overexpression. This confirms that by effectively increasing the NAD+ concentration within skin cells, the biological activity of exogenous NAD+ can be significantly enhanced.

[0026] The compositions of the present invention can be used to inhibit skin aging or inhibit photoaging.

[0027] In another aspect, the present invention provides a method for inhibiting skin aging or photoaging, comprising: applying a cosmetic composition containing nicotinamide adenine dinucleotide, glycyrrhetinic acid and quercetin to the skin of a subject.

[0028] In this invention, the term "object" can refer to all mammals, including humans, that require anti-aging, inhibition of photoaging, improvement of wrinkles, or skin regeneration.

[0029] In this invention, the term "application" means applying the composition of the invention to the skin of a subject, which can be done by conventional means of reaching the skin or being absorbed into the skin.

[0030] The cosmetic composition (cosmetic) of the present invention may further comprise all kinds of ingredients that can be used in cosmetics, such as moisturizers, such as glycerin, butylene glycol, propylene glycol, hexanediol, methyl gluceth-20, diglyceride, ethylhexylglycerin; UV blockers, such as ethylhexyl methoxycinnamate, ethylhexyl salicylate, ethylhexyl triazine, octocrylene, bis-ethylhexyloxyphenol methoxyphenyl triazine; pH adjusters, such as triethanolamine; thickeners, such as carbomer, xanthan gum, acrylate / C10-30 alkyl acrylate crosspolymers, hyaluronic acid; preservatives, such as phenoxyethanol, methylparaben, propylparaben; antioxidants, such as BHT, ethyl ascorbate ether, ascorbic acid; skin conditioning agents, such as β-glucan; surfactants, such as cetearyl glucoside, sorbitan stearate; fragrances or colorants, and there are no limitations on their composition.

[0031] The above-mentioned ingredients contained in the cosmetics of the present invention are preferably included in the cosmetic composition of the present invention within the range not exceeding the maximum usage amount specified in the "Cosmetic Safety Technical Specifications" formulated by each government.

[0032] The cosmetics of the present invention can be formulated into any dosage form commonly manufactured in the art. For example, the cosmetics may have the following dosage forms: toners such as softening lotions or nourishing lotions, spray-type toners, lotions such as facial lotions and body lotions, nourishing creams, moisturizing creams, eye creams, sticks, serums, cosmetic ointments, sprays, gels, masks, sunscreens, makeup primers, liquid or spray-type foundations, powders, makeup removers such as cleansing milks and cleansing oils, facial foams, soaps, shower gels, etc., but are not limited thereto.

[0033] In one specific implementation, the dosage form of the cosmetic product may be a balm, a water-in-oil (W / O) emulsion, an oil-in-water (O / W) emulsion, a solubilizing agent, or an oil-based dosage form.

[0034] The cosmetic of the present invention can be used according to conventional methods, and the frequency of use can be adjusted according to the user's skin condition or preference.

[0035] In one specific embodiment, it was confirmed that the cosmetic composition of the present invention can increase the concentration of niacinamide adenine dinucleotide in skin cells and provide anti-aging, skin texture improvement, wrinkle reduction, skin regeneration and barrier strengthening effects.

[0036] The advantages and features of the present invention, as well as the methods for implementing them, will become clear with reference to the experimental and preparation examples detailed below. However, the present invention is not limited to the experimental and preparation examples disclosed below, but can be implemented in many different forms. These are provided only to make the disclosure of the present invention more complete and to fully convey the scope of the present invention to those skilled in the art.

[0037] Invention Effects

[0038] When applied to the skin, the cosmetic composition of the present invention can increase the concentration of nicotinamide adenine dinucleotide in skin cells and provide anti-aging, skin texture improvement, wrinkle reduction, skin regeneration and barrier strengthening effects. Attached Figure Description

[0039] Figure 1 This is a photograph showing the cell aging results of Experimental Example 3 of the present invention.

[0040] Figure 2 This is a graph showing the results of the cell replication lifespan extension effect of Experimental Example 5 of the present invention. Detailed Implementation

[0041] The present invention will now be described in more detail through embodiments. These embodiments are only used to illustrate the present invention more specifically, and the scope of the present invention is not limited to these embodiments.

[0042] Example 1: Confirmation of CD38 expression inhibition ability

[0043] CD38 expression inhibition ability is an evaluation index for anti-aging and skin health improvement. In this experiment, we confirmed whether CD38 expression was inhibited when treated with the composition of the present invention, and the results are shown in Table 1.

[0044] Specifically, fibroblasts (HS68) were seeded in 12-well plates and cultured for 24 hours. The culture medium used was DMEM (Dulbecco's Modified Eagle Medium, Solbiopharm, Korea), and an incubator maintained at 37°C and 5% CO2 was used. After 24 hours, the medium was removed, and the cells were washed once with PBS (Phosphate Buffered Saline). After removing the PBS, DMEM containing 25 ppm NAD+ and the evaluation subjects shown in Table 1 was added to the cells. After 24 hours, the medium was removed again, and RNA was extracted using the AccuPrep® Universal RNA Extraction Kit (Bioneer, Korea). cDNA was then synthesized using the same RNA and cDNA synthesis kit (TOPscript™ cDNA Synthesis Kit (dN6 Mix), Enzynomics, Korea). Next, RT-qPCR (Real-time polymerase chain reaction) was performed using the TOPreal™ SYBR Green RT-qPCR kit and the Applied Biosystems™ StepOne™ Real-Time PCR System (Thermofisher, USA) to analyze CD38 expression levels. To confirm the impact of the evaluation target, DMEM containing 25 ppm NAD+ but without the evaluation target component (control) was also processed using the same method, and CD38 expression levels were analyzed. The CD38 inhibition rate relative to the control was then calculated using the following formula and is shown in Table 1.

[0045] CD38 inhibition rate (%) = [1 - (Result value of the evaluated subject ÷ Result value of the control)] × 100

[0046] [Table 1]

[0047] As shown in Table 1 above, it can be confirmed that NAD+-induced CD38 expression is significantly inhibited in the combination of quercetin and glycyrrhetinic acid, and in combination with sitosterol or tocopheryl acetate. In particular, the inhibition rates of Examples 6 and 8 of the present invention were confirmed to be significantly higher, demonstrating effectiveness in preventing and improving skin aging.

[0048] Experiment 2 confirmed an increase in intracellular NAD+.

[0049] NAD+ is a factor involved in intracellular metabolism and is associated with aging. The NAD+ / NADH ratio is an indicator that confirms how much NAD+ is retained without being converted into NADH; generally, a higher ratio indicates a greater inhibitory effect on skin aging. In this experimental example, it was confirmed whether the indicator increased or decreased when treated with the composition of the present invention, and the results are shown in Table 2.

[0050] Specifically, fibroblasts (HS68) were seeded in 12-well plates and cultured for 24 hours. The culture medium used was DMEM (Dulbecco's Modified Eagle Medium, Sol Biopharmaceutical, Korea), and an incubator maintained at 37°C and 5% CO2 was used. After 24 hours, the medium was removed, and the cells were washed once with PBS (Phosphate-Buffered Saline). After removing the PBS, DMEM containing 25 ppm NAD+ and the evaluation subjects shown in Table 2 was added to the cells. After 24 hours, the medium was removed, and the cells were washed three times with PBS. A kit capable of quantifying intracellular NAD+ and NADH at absorbance at 450 nm (PicoSens™ NAD / NADH Assay Kit, Biomax, Korea) was used. Quantification was performed according to the manufacturer's operating procedures, and the NAD+ / NADH ratio for each evaluation subject was calculated. At this point, to confirm the impact on the evaluation target, the same procedure was performed on DMEM (control) containing 25 ppm NAD+ but not the evaluation target component. Then, the improvement rate of the NAD+ / NADH ratio relative to the control was calculated according to the following formula and is shown in Table 2.

[0051] Improvement rate of NAD+ / NADH ratio (%)

[0052] = [(Result value of the evaluated object ÷ Result value of the control group) - 1] × 100

[0053] [Table 2]

[0054] As shown in Table 2 above, the NAD+ / NADH ratios of Examples 1, 2, 4 to 7 increased by a level exceeding expectations. In particular, Examples 6 and 7 showed significantly higher improvement rates, confirming that the topical skin agent of the present invention can increase the concentration of nicotinamide adenine dinucleotide in skin cells, thereby effectively inhibiting skin aging.

[0055] Experiment Example 3: Confirmation of the inhibitory effect on cell senescence

[0056] After treating fibroblasts with oxidative stress (H2O2) to increase cell senescence, they were treated with the composition of the present invention to confirm whether the senescence was improved. The results are shown in Table 3 and... Figure 1 middle.

[0057] Specifically, fibroblasts (HS68) were seeded in 96-well plates and cultured for 24 hours. The culture medium used was DMEM (Dulbecco's Modified Eagle Medium, Sol Biopharmaceutical, Korea), and an incubator maintained at 37°C and 5% CO2 was used. After 24 hours, the medium was removed, and the cells were washed once with PBS (Phosphate-Buffered Saline). After removing the PBS, DMEM containing 25 ppm NAD+ and the evaluation substances shown in Table 3 was added to the cells. After 4 hours, the cells were treated with 300 μM hydrogen peroxide (at final concentration), cultured for 2 hours, and then the medium was removed, followed by washing twice with PBS. DMEM containing the evaluation substances shown in Table 3 was added to the cells again, and the cells were cultured for 24 hours. After 24 hours, the medium was removed, and the cells were washed once with PBS. Subsequently, following the manufacturer's operating procedures, fluorescence intensity was measured using a Cellular Senescence Assay Kit (SPiDER-β Gal, Dojindo, Japan) at Ex: 535 nm; Em: 580 nm to quantify cell senescence. To confirm the influence of the evaluation subject, the following experimental groups were also tested using the same method: one group (Negative Control) with cells only receiving DMEM without the evaluation subject and without the hydrogen peroxide addition step; another group (Reference) with cells only receiving DMEM without the evaluation subject but with the hydrogen peroxide addition step; and a control group (Control) containing 25 ppm NAD+ but without the evaluation subject. The senescence improvement rate relative to the reference value was then calculated using the following formula.

[0058] Cellular aging improvement rate (%) =

[0059] [1 - (Result value of the evaluated object ÷ Result value of the benchmark value)] × 100

[0060] In experiments used to visualize aging, where green fluorescence increases with aging, a staining kit using SA-β-gal (SPiDER-βGal, Dojin, Japan) as an indicator of cell aging was employed. Staining was performed according to the manufacturer's instructions, and observation was conducted using a fluorescence microscope (EVOS™ M7000, Thermo Fisher Scientific, USA). The results after treatment with the composition of this invention are shown below. Figure 1 middle.

[0061] [Table 3]

[0062] As shown in Table 3 and Figure 1 As shown, after increasing oxidative stress (hydrogen peroxide treatment) in fibroblasts, the cells were treated with Examples 1, 3, 4, 8, and 9 of this invention to confirm whether cell senescence was improved. The results confirmed a synergistic effect beyond expectations, especially when Example 4 was applied. Figure 1 As shown, the aging-induced green fluorescence almost completely disappeared. Therefore, the cosmetic composition of the present invention is confirmed to be effective in inhibiting cell aging.

[0063] Experiment Example 4: Confirmation of the ability of ultraviolet light to inhibit MMP-1 expression

[0064] It was confirmed whether MMP-1 expression was inhibited when treated with the composition of the present invention after ultraviolet treatment to increase cellular MMP-1 expression.

[0065] Specifically, fibroblasts (HS68) were seeded into 12-well plates and cultured for 24 hours. The culture medium used was DMEM (Dulbecco's Modified Eagle Medium, Sol Biopharmaceutical, Korea), and an incubator maintained at 37°C and 5% CO2 was used. After 24 hours, the medium was removed, and the cells were washed once with PBS (Phosphate-Buffered Saline). After removing the PBS, DMEM containing 25 ppm NAD+ and the evaluation substances shown in Table 4 was added to the cells. After 4 hours of culture, the medium was removed, PBS was added to the cells, and they were then irradiated with ultraviolet light. Ultraviolet irradiation was performed using a BIO-SUN UV irradiator (Vilber Lourmat, France), irradiating 312 nm narrow-band UVB at 35 mJ / cm² and 15 mJ / cm². 2Cells were irradiated with narrow-band UVA at 365 nm. Afterward, PBS was removed, and DMEM containing the evaluation components shown in Table 4 was added to the cells again, followed by 48 hours of incubation. After 48 hours, the supernatant was collected, and MMP-1 expression was quantified by ELISA (Enzyme-linked immunosorbent assay). For this purpose, the Human Total MMP-1 DuoSet ELISA (R&D Systems, USA) was used, and the experiments were performed according to the manufacturer's instructions. Protein quantification was performed using the BCA assay (Pierce™ BCA Protein Assay Kits, Thermo Fisher Scientific, USA). To confirm the effect of the evaluation components, the same procedure was performed on the experimental group (negative control) where cells were added only to DMEM without the evaluation components and without the UV irradiation step, and on the control group containing 25 ppm NAD+ but without the evaluation components. The MMP-1 expression inhibition capacity relative to the control was then calculated using the following formula.

[0066] MMP-1 expression inhibition capacity (%) =

[0067] [1 - (Result value of the evaluated object ÷ Result value of the control group)] × 100

[0068] [Table 4]

[0069] As shown in Table 4, fibroblasts were treated with ultraviolet light to increase MMP-1 expression, and then treated with Example 1 of the present invention to confirm the prevention and improvement rate of cell aging. The results confirmed that Example 1 had an improved ability to inhibit MMP-1 expression. Therefore, the cosmetic composition of the present invention was confirmed to be effective in preventing skin aging and maintaining elasticity.

[0070] Experiment Example 5: Confirmation of the effect of extending cell replication lifespan

[0071] It was confirmed whether the composition of the present invention has the effect of prolonging cell division ability and replication life.

[0072] Specifically, fibroblasts (HS68) were seeded at 10 cells per well in 96-well plates and cultured for 24 hours. The culture medium used was DMEM (Dulbecco's Modified Eagle Medium, Sol Biopharmaceutical, Korea), and an incubator maintained at 37°C and 5% CO2 was used. After 24 hours, the medium was removed, and the cells were washed once with PBS (Phosphate Buffered Saline). After removing the PBS, DMEM containing 25 ppm NAD+ and the evaluation components shown in Table 5 was added to the cells. The medium was then changed every 7 days, and cell population and proliferation were measured on days 0, 21, and 28. To confirm the influence of the evaluation components, the negative control group (treated only with DMEM) and the control group (containing 25 ppm NAD+ but not the evaluation components) were also treated and measured using the same method. The assay was performed using the CCK-8 assay (CK04, Dojin, Japan), and the results are shown below. Figure 2 In this case, the relative cell population is directly proportional to the cell number. Based on the measured values, the effect of extending cell division capacity and replication lifespan is calculated according to the following formula and is shown in Table 5.

[0073] Effectiveness (%) = [1 - (Result value of the evaluated subject ÷ Result value of the negative control)] × 100

[0074] [Table 5]

[0075] As shown in Table 5 and Figure 2 As shown, the triple combination as in Examples 6 and 7 confirmed the effect of prolonging cell division capacity and replication lifespan. Therefore, the cosmetic composition of the present invention has been confirmed to be effective in inhibiting cell aging, improving skin texture, promoting skin regeneration, and strengthening the skin barrier.

[0076] Based on the embodiments and experimental examples of the present invention, it has been confirmed that the cosmetic composition of the present invention provides aging improvement effects on damaged skin and skin wrinkle and elasticity improvement effects by increasing the concentration of nicotinamide adenine dinucleotide in skin cells.

[0077] From the above description, those skilled in the art should understand that the present invention can be implemented in other specific forms without changing its technical concept or essential features. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. The scope of the present invention should be interpreted as including, compared to the detailed description above, all changes or modifications derived from the meaning and scope of the claims described below, and from their equivalents.

Claims

1. A cosmetic composition comprising nicotinamide adenine dinucleotide, glycyrrhetinic acid, and quercetin.

2. The cosmetic composition according to claim 1, wherein, The cosmetic composition further comprises sitosterol or tocopheryl acetate.

3. The cosmetic composition according to claim 1, wherein, It contains the nicotinamide adenine dinucleotide and glycyrrhetinic acid in a weight ratio of 1:0.0004 to 1:0.

4.

4. The cosmetic composition according to claim 1, wherein, It contains the nicotinamide adenine dinucleotide and quercetin in a weight ratio of 1:0.0004 to 1:0.

4.

5. The cosmetic composition according to claim 2, wherein, It contains the nicotinamide adenine dinucleotide and sitosterol in a weight ratio of 1:0.0004 to 1:0.

4.

6. The cosmetic composition according to claim 2, wherein, It contains the nicotinamide adenine dinucleotide and tocopherol acetate in a weight ratio of 1:0.0004 to 1:

1.

7. The cosmetic composition according to claim 1, wherein, The composition is used to inhibit skin aging.

8. The cosmetic composition according to claim 1, wherein, The composition is used to inhibit photoaging.

9. A method for inhibiting skin aging, comprising: The step of applying a cosmetic composition containing nicotinamide adenine dinucleotide, glycyrrhetinic acid and quercetin to the skin of a target body.

10. A method for inhibiting photoaging, comprising: The step of applying a cosmetic composition containing nicotinamide adenine dinucleotide, glycyrrhetinic acid and quercetin to the skin of a target body.

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