Light guide assembly for a hemolysis device for blood samples

Abstract

A light guide assembly (7) for a blood sample hemolysis device (1) is disclosed. The light guide assembly (7) includes a neon lamp bulb (10), a light-emitting diode / LED (8) including a reflector element (11), and a light guide (9). The LED (8) is mounted in the light guide (9) such that the light guide (9) spatially fixes the LED (8), and the light guide (9) is configured to transmit light emitted from the neon lamp bulb (10) through the reflector element (11) of the LED (8) when mounted in the device (1). A blood sample hemolysis device (1) utilizing such a light guide assembly (7) and a method for assembling such a device (1) are also disclosed.

Description

Technical Field

[0001] This invention relates to a photoconductive component for a blood sample hemolysis apparatus. The photoconductive component is used to optically analyze a blood sample before and / or after hemolysis of a blood sample contained in the apparatus, for example by optically measuring at least one parameter of the blood sample. Background Technology

[0002] Devices for hemolyzing blood samples and measuring at least one parameter thereof (sometimes referred to as "hemolyzers") are known in the art. In such devices, an ultrasonic device for generating ultrasound waves may be arranged at or near the sample chamber (e.g., a cuvette) to induce hemolysis of the blood sample contained within the sample chamber by means of ultrasound waves. Furthermore, such devices may include an optical measuring device, for example in the form of a suitable light source (e.g., a light-emitting diode (LED)), arranged on one side of the sample chamber, and a suitable optical detector arranged on the opposite side of the sample chamber to allow optical measurement of at least one parameter of the blood sample. An example of a blood sample hemolysis device is described in WO 2010 / 006603 A1.

[0003] To calibrate the optical measuring apparatus, in addition to the light source of the optical measuring apparatus, the hemolyzer may also be equipped with a calibration light source, for example, in the form of a neon lamp. The calibration light source should be arranged in the apparatus such that light emitted from both the calibration light source and the measuring light source reaches the sample chamber. Placing the calibration light source between the measuring light source and the sample chamber may be considered unsuitable because this could cause the calibration light source to partially block light emitted from the measuring light source, potentially leading to inaccurate and / or unreliable measurements. Therefore, it is preferable to place the measuring light source between the calibration light source and the sample chamber. However, this results in the measuring light source, particularly its reflector element, at least partially blocking light emitted from the calibration light source. This could result in insufficient calibration light reaching the sample chamber to achieve proper calibration of the optical measuring system. Summary of the Invention

[0004] One object of embodiments of the present invention is to provide a blood sample hemolysis device, and a photoconductor assembly for such a device, wherein accurate and reliable calibration can be easily obtained.

[0005] According to a first aspect, the present invention provides a light guide assembly for a blood sample hemolysis device, the light guide assembly comprising a neon lamp bulb, a light-emitting diode (LED) including a reflector element, and a light guide, wherein the LED is mounted in the light guide such that the light guide spatially fixes the LED, and wherein the light guide is configured to, when mounted in the device, transmit light emitted from the neon lamp bulb through the reflector element of the LED.

[0006] Therefore, according to a first aspect, the present invention provides a light guiding assembly comprising a neon bulb, a light-emitting diode (LED), and a light guide. In this context, the term "light guide" should be interpreted as meaning an element or component capable of guiding light (at least within a certain wavelength range) through it. Thus, the light guide is capable of transmitting light and is therefore at least partially transparent to light, at least to certain wavelengths.

[0007] The LED includes a reflector element that reflects a portion of the light emitted from the LED to ensure that a larger portion of the emitted light is directed toward the sample chamber. This will be described in further detail below. The LED is mounted in a light guide. Therefore, the LED and the light guide are spatially fixed relative to each other; that is, the light guide spatially fixes the LED. This will also be described in further detail below.

[0008] The light guide is also configured to allow light emitted from the neon bulb to pass through the reflector element of the LED. Therefore, the light guide is transparent to light emitted from the neon bulb; in this sense, most of the light emitted from the neon bulb is transmitted through the light guide, and only a small portion of the light is reflected or scattered within the light guide. Thus, it essentially only prevents the portion of the light emitted from the neon bulb that is blocked by the LED, particularly by the reflector element of the LED, from passing through the light guide in which the LED is mounted.

[0009] Therefore, light guides serve the dual purpose of spatially fixing LEDs and transmitting light emitted from neon bulbs.

[0010] The light guide assembly is configured to form part of or be installed within a blood sample hemolysis device (i.e., essentially the device as described above). When the light guide assembly is installed in such a device, it can form part of the device's optical measurement system. In particular, an LED can form the measurement light source, and a neon lamp can form the calibration light source. Therefore, when the light guide assembly is installed in the blood sample hemolysis device, the light emitted from the LED is preferably guided toward the sample chamber. Since the light guide spatially fixes the LED, it ensures that the LED is securely and properly held in place relative to and within the device, thus ensuring accurate and reliable optical measurement of at least one relevant parameter of the blood sample contained in the device. For example, the spatial fixation of the LED by the light guide ensures that the light emitted from the LED passes through the optimal optical path of the device.

[0011] In addition, the light guide ensures that the light emitted from the neon lamp bulb is properly transmitted through the reflector element of the LED, thus ensuring that sufficient calibration light can reach the sample chamber and can be further transmitted to the calibration unit.

[0012] Therefore, the light guide, which serves the dual purpose of spatially fixing the LED and transmitting light emitted from the neon lamp, ensures that optical measurements of relevant parameters of blood samples can be reliably and accurately obtained based on the light emitted from the LED, while ensuring that sufficient calibration light from the neon lamp reaches the sample chamber, thus ensuring proper and accurate calibration of the optical measurement system. Furthermore, the dual-purpose light guide provides an easy, reliable, and cost-effective way to achieve this.

[0013] The light guide can be configured to transmit light with substantially no attenuation through it in the wavelength range of 400 nm to 750 nm (e.g., in the range of 450 nm to 700 nm, or in the range of 475 nm to 675 nm). This ensures that wavelengths primarily emitted from the neon lamp are allowed to pass through the light guide substantially unimpeded, meaning that most of the emitted light is transmitted through the light guide, and only a small portion is scattered or reflected by the light guide or within the light guide itself. Therefore, it is ensured that the portion of the light emitted from the neon lamp that is not blocked by the reflective elements of the LED is effectively allowed to pass through the light guide substantially unimpeded, thus reaching the sample chamber and being usable for calibration purposes.

[0014] The light guide can be configured such that at least 40%, such as at least 50%, such as at least 60%, such as at least 70%, of the light emitted from the neon lamp bulb is transmitted through the reflector element of the LED. The portion of the emitted light that is not transmitted through the reflector element of the LED may be primarily due to the reflector element of the LED blocking the emitted light. According to this embodiment, sufficient calibration light is effectively ensured even when high output or intensity from the neon lamp bulb is not required.

[0015] The reflector element of the LED can define an area in a plane substantially perpendicular to the direction of light emitted from the neon bulb, said area being at most 70%, such as at most 60%, such as at most 50%, of the total cross-sectional area of ​​the light guide and the LED at the location of the reflector element. According to this embodiment, as seen from the neon bulb, the area blocked by the reflector element of the LED is relatively small compared to the total cross-sectional area defined by the light guide in which the LED is mounted. More specifically, at least 30% of the total cross-sectional area is left for the free passage of light emitted from the neon bulb, i.e., for transmission through the light guide and past the reflector element of the LED. This also ensures that sufficient calibration light is obtained.

[0016] Light emitted from a neon lamp and transmitted through a light guide and reflector element can be substantially parallel to light emitted from an LED. According to this embodiment, when the light guide assembly is installed in a blood sample hemolysis device, light emitted from the neon lamp and light emitted from the LED are guided substantially in the same direction (e.g., towards the sample chamber).

[0017] The LED can be embedded in a light guide. According to this embodiment, the LED is at least partially surrounded by the light guide and thus spatially secured by it.

[0018] The light guide may include a through-hole, and the LED can be mounted in the through-hole. For example, the LED can be tightly fitted into the through-hole. This will also ensure that the LED is securely fixed in space by the light guide.

[0019] Optical guides can be made of polycarbonate. For example, optical guides can be made of Lexan. ® (such as Lexan) ® 124 R or Lexan ® HF 1130 R) is used. Alternatively, the light guide can be made of polyamide (such as Grilamid). ® For example, Grilamid ® It is made of TR55. Such materials are inexpensive and easy to form into desired shapes. For example, light guides can be formed by injection molding. In addition, the optical properties of these materials allow light of relevant wavelengths to pass through the material, thus making them suitable for use as light guides.

[0020] The light guide may include a main body portion housing the LED and a mounting portion extending away from the main body portion, and at least a portion of the light emitted from the neon bulb and transmitted through the light guide via the reflector element may pass through the mounting portion.

[0021] According to this embodiment, the light guide includes two parts: a main body and a mounting part. The main body houses the LED, for example, the LED can be embedded in the main body. The main body can, for example, have a substantially cylindrical shape, with the LED mounted along its central axis.

[0022] The mounting portion extends away from the main body. When the main body has a substantially cylindrical shape, the mounting portion can extend radially away from the main body, that is, in a direction substantially perpendicular to the central axis of the substantially cylindrical main body. Therefore, the cross-sectional area of ​​the light guide is larger in the region defined by the mounting portion than in the region defined by the main body. Thus, the mounting portion represents the portion of the light guide not blocked by the LED, particularly not blocked by the LED's reflector element. The mounting portion can be arranged at the end of the main body, in which case it can be considered as the "foot" of the light guide.

[0023] Therefore, the mounting portion forms part of the light guide, through which light emitted from the neon bulb can pass appropriately without being blocked by the reflector element of the LED. However, it is not excluded that at least some of the light emitted from the neon bulb may also pass through the portion of the main body that is not blocked by the reflector element of the LED.

[0024] The mounting portion can also form part of the light guide, which the operator can easily grip to mount the light guide along with the LED in the blood sample hemolysis device. Furthermore, the mounting portion can form an adjacent surface facing other parts of the device, thus ensuring correct and accurate installation of the light guide assembly within the device.

[0025] According to a second aspect, the present invention provides a blood sample hemolysis apparatus, the apparatus comprising a sample chamber for receiving the blood sample, a hemolyzer unit, and a light guide assembly according to a first aspect of the invention, wherein the light guide assembly is positioned adjacent to the sample chamber such that light emitted from the LED and light emitted from the neon lamp and transmitted by the light guide through the reflector element of the LED reach the sample chamber.

[0026] In this context, the term "blood sample hemolysis device" should be interpreted as a device capable of causing hemolysis of a blood sample contained therein (e.g., by means of ultrasound) and for measuring at least one relevant parameter of the blood sample (e.g., by means of an optical measurement system).

[0027] The apparatus according to a second aspect of the invention includes a sample chamber for receiving a blood sample, a hemolysin unit, and a photoconductive assembly according to a first aspect of the invention. Therefore, the comments set forth above with reference to the first aspect of the invention are equally applicable here.

[0028] The sample chamber can be, for example, in the form of a cuvette, and can be provided with sidewalls, for example, in the form of a window. The volume of the sample chamber can range from 0.1 μl to 20.0 μl, preferably about 1 μl. The distance between the opposite sidewalls of the sample chamber can range from 0.05 mm to 0.30 mm, preferably about 0.1 mm.

[0029] A hemolyzer unit is part of a device that causes hemolysis of a blood sample contained in a sample chamber. For example, the hemolyzer unit may include an ultrasonic device for generating ultrasonic waves in the sidewall of the sample chamber, thus transmitting the ultrasonic waves to the blood sample contained in the sample chamber and causing hemolysis. The ultrasonic device may be arranged adjacent to the sidewall of the sample chamber (e.g., adjacent to the sidewall of the sample chamber) to ensure effective transmission of ultrasonic waves to the blood sample. For example, the ultrasonic device may be adapted to oscillate at a frequency in the range of 20 kHz to 100 kHz, preferably at approximately 30 kHz.

[0030] An ultrasonic device may include at least one piezoelectric element attached to the side wall of the sample chamber. Piezoelectric elements are inexpensive, and their use makes the activation of the ultrasonic device highly controllable.

[0031] To increase the inertia of reciprocating resonance, the hemolytic unit may also include at least one mass element coupled to the ultrasound device. The at least one mass element may include at least one spring element that elastically fixes the at least one mass element to, for example, the housing of the device.

[0032] The light guide assembly is positioned adjacent to the sample chamber in such a manner that light emitted from the LED and light emitted from the neon lamp and transmitted through the reflector element of the LED by the light guide reach the sample chamber. Therefore, the LED can be used as the measurement light source for the device's optical measurement system, and the neon lamp can be used as the calibration light source for the optical measurement system. As described above, the light guide, which spatially fixes the LED, ensures that the LED is firmly and accurately positioned relative to the device, particularly relative to the sample chamber, and is specifically positioned to provide the optimal optical path for the light emitted from the LED through the sample chamber and toward the optical sensor arranged on the opposite side of the sample chamber. Simultaneously, and due to the dual purpose of the light guide, it ensures that light emitted from the neon lamp is transmitted through the light guide and thus reaches the sample chamber sufficiently to ensure that proper and accurate calibration of the optical measurement system can be performed.

[0033] The light guide of the light guide assembly can be installed in a part of the hemolysing unit. For example, the light guide can be installed in an ultrasound device, such as in the moving mass of the ultrasound device. This ensures that the light guide, and therefore the other parts of the light guide assembly, are properly positioned relative to the sample chamber.

[0034] According to a third aspect of the present invention, a method for assembling a hemolysis device for blood samples is provided, the method comprising the following steps:

[0035] - The hemolysing unit is installed together with the sample chamber in the housing of the device.

[0036] - A light-emitting diode (LED) with a reflector element is mounted in the light guide.

[0037] - The light guide, together with the LED and the reflector element, is mounted in the housing adjacent to the sample chamber, and

[0038] - Mount the neon bulb adjacent to the light guide.

[0039] An apparatus assembled by means of the method according to the third aspect of the invention can be an apparatus according to the second aspect of the invention. Therefore, the comments set forth above with reference to the first and second aspects of the invention are equally applicable here.

[0040] In the method according to a third aspect of the invention, the hemolyzer unit is initially mounted together with the sample chamber in the housing of the device. The hemolyzer unit may include an ultrasonic device arranged adjacent to (i.e., immediately adjacent to, such as adjacent to or in contact with) the sample chamber. This allows, during use, a blood sample contained in the sample chamber to be hemolyzed by the hemolyzer unit. The sample chamber and the hemolyzer unit may be, for example, of the type described above with reference to the second aspect of the invention.

[0041] Furthermore, a light-emitting diode (LED) having a reflector element is mounted in a light guide of the type described above with reference to the first aspect of the invention. This can be accomplished by mounting the reflector element on the LED and then mounting the LED having the reflector element in the light guide. Alternatively, the LED can be mounted in the light guide, and then the reflector element can be mounted on the LED in the light guide.

[0042] Next, the light guide, along with the LED and reflector elements, is mounted in the housing adjacent to the sample chamber (i.e., immediately adjacent to the sample chamber, for example, adjacent to the sample chamber or to a portion of the hemolysin unit, which may in turn be arranged adjacent to the sample chamber). This will allow light emitted from the LED to reach the sample chamber, enabling optical measurement of at least one parameter of the blood sample contained within the sample chamber.

[0043] Finally, the neon lamp is mounted adjacent to the light guide. Therefore, when in use, the light emitted by the neon lamp can pass through the light guide, through the reflector element of the LED, and reach the sample chamber, enabling calibration of the optical measurement system including the LED.

[0044] The step of mounting the light guide together with the LED and reflector elements in the housing may include mounting the light guide in a portion of the hemolysin unit. As described above with reference to a second aspect of the invention, this may include mounting the light guide in the ultrasound device of the hemolysin unit, for example, in the mass element of the ultrasound device. Attached Figure Description

[0045] The invention will now be described in further detail with reference to the accompanying drawings, in which...

[0046] Figure 1 and 2 A blood sample hemolysis apparatus according to an embodiment of the present invention is shown.

[0047] Figure 3 The LED and neon bulbs of a prior art optical measurement system are shown.

[0048] Figure 4 An optical guide assembly according to an embodiment of the present invention is shown.

[0049] Figure 5 and6 This is a perspective view of a light guide assembly according to an embodiment of the present invention, and

[0050] Figure 7-10 An optical guide for an optical guide assembly according to an embodiment of the present invention is shown. Detailed Implementation

[0051] Figure 1 and 2 A blood sample hemolysis apparatus 1 according to an embodiment of the present invention is shown. Figure 1 This is a perspective view of device 1, and Figure 2 This is a cross-sectional view of device 1.

[0052] The device 1 includes a housing 2 encapsulating the internal portion of the device 1, and a sample chamber 3 in the form of a cuvette for receiving a blood sample to be hemolyzed. The device 1 also includes a hemolyzer unit comprising an ultrasonic device 4 in the form of a piezoelectric element arranged in contact with a side wall 5 of the sample chamber 3, and a mass element 6 coupled to the corresponding ultrasonic device 4. Therefore, vibrations from the ultrasonic device 4 are transmitted to the side wall 5, and thus to the blood sample contained in the sample chamber 3, to induce hemolysis of the blood sample. The mass element 6 increases the inertia of the reciprocating resonance, thus enhancing the transmission of vibration from the ultrasonic device 4 to the side wall 5.

[0053] According to an embodiment of the invention, a light guide assembly 7 is mounted in one of the mass elements 6. The light guide assembly 7 includes an LED 8 mounted in a light guide 9 and a neon bulb 10 arranged adjacent to the light guide 9 (i.e., immediately adjacent to the light guide 9, such as adjacent to the light guide). Figure 2 In this configuration, a neon lamp 6 is positioned below or beneath a light guide 9. The light guide 9 spatially fixes the LED 8 and is configured to transmit light emitted from the neon lamp 10 through a reflector element (not shown) of the LED 8. Therefore, light emitted from the LED 8 and light emitted from the neon lamp 10 can reach the sample chamber 3.

[0054] The LED 8 forms the measuring light source of the apparatus 1, and the neon lamp 10 forms the calibration light source of the apparatus 1. (Refer to the following text.) Figure 3-6 The optical guide component 7 is described in further detail.

[0055] Figure 3 The diagram illustrates an existing optical measurement system with an LED 8 and a neon lamp 10. The LED 8 includes a reflector element 11. It can be seen that the LED 8 is relatively large, and it will be directly mounted to... Figure 1 and 2In the mass element 6 of the device 1 shown, the reflector element 11 is also relatively large, and thus, as indicated by the arrow, most of the light emitted from the neon lamp bulb 10 is blocked by the reflector element 11 and is therefore prevented from reaching the sample chamber. Therefore, in order to ensure sufficient calibration light for proper and reliable calibration, the neon lamp bulb 10 needs to be able to emit light of a certain intensity.

[0056] Figure 4 A light guide assembly 7 according to an embodiment of the present invention is shown, which includes an LED 8 having a reflector element 11 and a neon bulb 10. Figure 4 LED 8 is significantly smaller than Figure 3 The LED 8 is smaller, and therefore the reflector element 11 is also significantly smaller. This results in a smaller portion of the light emitted from the neon bulb 10 being blocked by the reflector element 11, and thus a larger portion of the emitted light reaching the sample chamber. Therefore, proper and reliable calibration can be obtained without imposing additional requirements on the intensity of the light emitted from the neon bulb 10.

[0057] The small size of LED 8 makes it impossible to directly assemble it into... Figure 1 and 2 The mass element 6 of device 1 is used. Therefore, LED 8 is mounted in a light guide (not shown), which spatially fixes LED 8, thus ensuring an optimal optical path for the light emitted from LED 8. Furthermore, the light guide is configured to transmit light emitted from the neon lamp 10, thus ensuring that light emitted from the neon lamp 10 and not blocked by the reflector element 11 of LED 8 is allowed to reach the sample chamber. For example, the light guide can be substantially transparent to light having a wavelength corresponding to the light emitted from the neon lamp 10.

[0058] Figure 5 and 6 An optical guide component 7 according to an embodiment of the present invention is shown. Figure 5 This is a perspective view of the light guide component 7, and Figure 6 This is a partial cross-sectional view of the light guide component 7, revealing details of the LED 8 within the light guide component 7.

[0059] The light guide assembly 7 includes an LED 8 mounted in a light guide 9 and a neon bulb 10 arranged adjacent to the light guide 9. The light guide 9 includes a main body portion 9a and a mounting portion 9b. The main body portion 9a is provided with a through hole 12, in which the LED 8 is mounted, and the LED 8 is tightly fitted in the through hole 12. This securely fixes the LED 8 in space relative to the light guide 9.

[0060] The main body portion 9a has a substantially cylindrical shape, and the mounting portion 9b extends radially away from the main body portion 9a. Therefore, the mounting portion 9b can be considered to form the "feet" of the light guide 9. When the light guide 9, in which the LED 8 is mounted, is being installed in the blood sample hemolysis device, the mounting portion 9b can be grasped by the person performing the installation. This ease of manipulation of the light guide 9 allows for easy and reliable installation. Furthermore, the mounting portion 9b can be oriented towards other parts of the device (e.g., towards...). Figure 2 The adjacent surface of the mass element 6 shown in the figure ensures that the light guide 9 and thus the light guide assembly 7 are accurately installed in the device.

[0061] The light guide 9 is configured to allow light emitted from the neon bulb 10 to pass through a reflector element (not shown) of the LED 8. For example, the light guide 9 may be transparent to light emitted from the neon bulb 10. In particular, light emitted from the neon bulb 10 may pass through the mounting portion 9b of the light guide 9.

[0062] Figure 7-10 An optical guide 9 for an optical guide assembly according to an embodiment of the present invention is shown. Figure 7 This is a perspective view of light guide 9. Figure 8 This is a top view of the optical guide 9, and Figure 9 and 10 This is a side view of the light guide 9. The main body 9a, the mounting part 9b, and the through hole 12 can be clearly seen.

Claims

1. A light guide assembly (7) for a blood sample hemolysis device (1), the light guide assembly (7) comprising a neon lamp bulb (10), a light-emitting diode (LED) (8) including a reflector element (11), and a light guide (9), wherein the LED (8) is mounted in the light guide (9) such that the light guide (9) spatially fixes the LED (8), and wherein the light guide (9) is configured to, when mounted in the device (1), transmit light emitted from the neon lamp bulb (10) through the reflector element (11) of the LED (8).

2. The light guide assembly (7) according to claim 1, wherein the light guide (9) is configured to transmit light with substantially no attenuation in the wavelength range of 400 nm to 750 nm through the light guide (9).

3. The light guide assembly (7) according to claim 1 or 2, wherein the light guide (9) is configured to transmit at least 40% of the light emitted from the neon bulb (10) through the reflector element (11) of the LED (8).

4. The light guide assembly (7) according to any one of the preceding claims, wherein the reflector element (11) of the LED (8) defines an area in a plane substantially perpendicular to the direction of light emitted from the neon bulb (10), the area being at most 70% of the total cross-sectional area of ​​the light guide (9) and the LED (8) at the location of the reflector element (11).

5. The light guide assembly (7) according to any one of the preceding claims, wherein light emitted from the neon bulb (10) and transmitted by the light guide (9) through the reflector element (11) is substantially parallel to light emitted from the LED (8).

6. The light guide assembly (7) according to any one of the preceding claims, wherein the LED (8) is embedded in the light guide (9).

7. The light guide assembly (7) according to any one of the preceding claims, wherein the light guide (9) includes a through hole (12), and wherein the LED (8) is mounted in the through hole (12).

8. The light guide assembly (7) according to any one of the preceding claims, wherein the light guide (9) is made of polycarbonate.

9. The light guide assembly (7) according to any one of the preceding claims, wherein the light guide (9) includes a body portion (9a) housing the LED (8) and a mounting portion (9b) extending away from the body portion (9a), and wherein at least a portion of the light emitted from the neon bulb (10) and transmitted by the light guide (9) through the reflector element (11) passes through the mounting portion (9b).

10. The light guide assembly (7) according to any one of the preceding claims, wherein the neon bulb (10) is a calibration light source, and wherein the LED (8) is a measurement light source.

11. The light guide assembly (7) according to any one of the preceding claims, wherein the light guide (9) is configured to spatially fix the LED (8) such that an optimal optical path is obtained for light emitted from the LED (8).

12. A blood sample hemolysis device (1), the device (1) comprising a sample chamber (3) for receiving the blood sample, a hemolyzer unit, and a light guide assembly (7) according to any one of the preceding claims, wherein the light guide assembly (7) is positioned adjacent to the sample chamber (3) such that light emitted from the LED (8) and light emitted from the neon bulb (10) and transmitted by the light guide (9) through the reflector element (11) of the LED (8) reach the sample chamber (3).

13. The blood sample hemolysis apparatus (1) according to claim 12, wherein the light guide (9) of the light guide assembly (7) is installed in a portion of the hemolyzer unit.

14. A method for assembling a blood sample hemolysis device (1), the method comprising the following steps: - The hemolysin unit and the sample chamber (3) are installed together in the housing (2) of the device (1). - A light-emitting diode (LED) (8) with a reflector element (11) is mounted in a light guide (9). - The light guide (9), together with the LED (8) and the reflector element (11), is mounted adjacent to the sample chamber (3) in the housing (2), and - Install the neon bulb (10) adjacent to the light guide (9).

15. The method of claim 14, wherein the step of mounting the light guide (9) together with the LED (8) and the reflector element (11) in the housing (2) comprises mounting the light guide (9) in a portion of the hemolytic unit.

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