A cutting management method for promoting growth of acanthopanax cuttings

Abstract

The application provides a cutting management method for promoting growth of acanthopanax senticosus cuttings, and relates to the technical field of acanthopanax senticosus cutting seedling raising. The cutting management method is characterized in that, on the basis of conventional management, the cutting substrate is sprinkled with a discarded flue-cured tobacco stalk extraction solution, and after the cutting, a nutrient agent composed of calcium superphosphate, borax, a justicia procumbens extract and 5-amino levulinic acid is sprayed. The application overcomes the shortcomings of the prior art, effectively promotes root formation of acanthopanax senticosus, promotes bud point germination, promotes new shoots to form new branches, further promotes branch growth, and improves the efficiency and effect of seedling raising.

Description

Technical Field

[0001] This invention relates to the field of Acanthopanax senticosus cutting propagation technology, specifically to a cutting management method that promotes the growth of Acanthopanax senticosus cuttings. Background Technology

[0002] Currently, there are four main methods for artificially propagating Acanthopanax senticosus: sowing, cutting, root division (tillering), and tissue culture. Among these, sowing is difficult because the dormancy of Acanthopanax senticosus seeds is hard to break, and the overall germination rate is low, resulting in low germination efficiency. Although root division (tillering) produces seedlings with fast growth and a high survival rate, the overall propagation coefficient is extremely low, and the number of seedlings that can be obtained is very limited, which cannot meet the needs of large-scale planting. Tissue culture has a high propagation coefficient and a short cycle, but it requires high technical skills and is costly, requiring a sterile environment, specialized equipment and reagents, and highly skilled operators. Cutting propagation is currently the most mature method for propagating Acanthopanax senticosus, and it can maintain the excellent traits of the parent plant, but there is a problem with rooting during the cutting propagation period.

[0003] Currently, the methods to improve the rooting efficiency of Acanthopanax senticosus cuttings are mostly to treat the cuttings with rooting hormones during the cutting period and to apply appropriate plant hormones after cutting, which can improve the rooting efficiency of Acanthopanax senticosus to a certain extent. However, in current practical applications, there are problems where Acanthopanax senticosus cuttings root successfully but new shoots grow slowly or not at all, which seriously affects the seedling cultivation effect. Therefore, how to improve the growth of Acanthopanax senticosus seedlings is a major research direction for cutting propagation at this stage. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a cutting management method that promotes the growth of Acanthopanax senticosus cuttings. This method effectively enhances the rooting of Acanthopanax senticosus while promoting bud sprouting and the formation of new branches from new buds, thereby further promoting branch growth and improving the efficiency and effectiveness of seedling cultivation.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for promoting the growth of Acanthopanax senticosus cuttings, the method comprising the following steps: S1. Preparation of cuttings: Cut semi-lignified or lignified branches from the current year, disinfect them, and soak them in rooting hormone to obtain pre-treated cuttings for later use. S2. Treatment of cutting substrate: Spray disinfectant on the cutting substrate and immerse it in waste flue-cured tobacco straw extract, then cover it with film for 2-3 days to obtain pretreated substrate. The waste flue-cured tobacco straw extract is an extract obtained by water extraction of waste flue-cured tobacco straw at a temperature below 30℃. S3. Cutting treatment: Insert the pre-treated cuttings into the substrate and water them thoroughly. After 2-3 days, spray the substrate at the lower end of the cuttings with a nutrient solution. The nutrient solution is formulated as follows: 3-5 g / L superphosphate, 2-4 g / L borax, 400-600 mg / L of *Gnaphalium affine* extract, and 40-100 mg / L of 5-aminolevulinic acid. S4. Daily management: Control the temperature and humidity appropriately, water in a timely manner, and apply foliar fertilizer every 15-20 days after cutting.

[0006] Preferably, in step S1, the length of the pretreated cutting is 15-20cm, and 2-3 buds are retained on the pretreated cutting.

[0007] Preferably, in step S1, the method of soaking the branches in the rooting agent after disinfection is to soak the branches in a 50% carbendazim wettable powder solution diluted 800-1000 times for 10-12 minutes, rinse them with clean water, and then immerse the lower end in an indoleacetic acid solution with a mass concentration of 200-300 mg / L for 3-5 minutes.

[0008] Preferably, the disinfectant sprayed in step S2 is a 50% carbendazim wettable powder solution diluted 800-1000 times, and the amount of disinfectant sprayed is 0.8-1.2 L / m³. 2 Furthermore, the amount of waste tobacco straw extract added is 1-1.5 L / m³. 2 .

[0009] Preferably, the specific preparation method of the waste flue-cured tobacco straw extract in step S2 is as follows: after crushing the waste flue-cured tobacco straw, add water at a material-to-liquid ratio of 1:80-120, stir thoroughly, soak at a temperature below 30°C for 2-3 days, and then press and filter. The filtrate is the waste flue-cured tobacco straw extract.

[0010] Preferably, the preparation method of the mouse thorn extract in step S3 includes the following steps: S3-1. Wash the whole plant of *Gnaphalium affine*, add water and grind it into a paste for later use; S3-2, Adjust the pH of the slurry to 5.5-6, then use... 60 The slurry was pretreated by Co-γ irradiation for 30-60 seconds, and then the pH was adjusted to neutral. S3-3. Add ethanol to the pretreated slurry, adjust the ethanol concentration to 40%-50%, extract for 1-1.5 hours, then press and filter, and dry the filtrate to obtain the extract of *Gnaphalium affine*.

[0011] Preferably, the absorbed dose of the irradiation treatment in step S3-2 is 3-6 kGy.

[0012] Preferably, in step S4, the appropriate temperature and humidity are controlled as follows: the temperature is controlled at 18-25℃ and the relative humidity is controlled at 80%-90%.

[0013] Preferably, in step S4, foliar fertilization is performed by spraying with a 0.2% potassium dihydrogen phosphate solution.

[0014] This invention provides a method for managing cuttings to promote the growth of Acanthopanax senticosus cuttings, which has the following advantages compared with the prior art: This invention involves disinfecting the cutting substrate and then treating it with an extract from waste flue-cured tobacco straw. Following the insertion of Acanthopanax senticosus cuttings, a nutrient solution containing extracts of *Gnaphalium affine* and 5-aminolevulinic acid is applied. This effectively ensures root development of the cuttings while promoting bud sprouting and ensuring the growth of new shoots, thus improving the growth efficiency and effectiveness of the cuttings. The application of 5-aminolevulinic acid to the nutrient solution promotes the growth of the cuttings while effectively preventing the elongation of new shoots from the buds, thus preventing the continuous emergence of new shoots that consume nutrients and ensuring the normal growth of the cuttings. Attached Figure Description

[0015] Figure 1 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in experimental group 1 of this invention after 60 days of propagation. Figure 2 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in experimental group 2 of this invention after 60 days of propagation. Figure 3 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in experimental group 3 of this invention after 60 days of propagation. Figure 4 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in experimental group 4 of this invention after 60 days of propagation. Figure 5 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in the experimental group of this invention after 60 days of propagation. Figure 6 This is a schematic diagram showing the growth of a single Acanthopanax senticosus cutting in experimental group 7 of this invention after 60 days of propagation. Figure 7 This is a schematic diagram of the overall growth of Acanthopanax senticosus cuttings in experimental group 1 of this invention, which were 60 days old. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] The raw material preparation methods used in the following examples are as follows: 1. Preparation of extract from waste flue-cured tobacco straw: After crushing the waste flue-cured tobacco straw, add water at a material-to-liquid ratio of 1:100, stir thoroughly, soak at 25℃ for 3 days, then press and filter. The filtrate is the waste flue-cured tobacco straw extract.

[0018] 2. Preparation of extract 1 of *Gnaphalium affine*: (1) Wash the whole plant of *Gnaphalium affine* and add twice the volume of water to grind it into a paste for later use; (2) Adjust the pH of the slurry to 5.5, and then use... 60 Co-γ rays were used to treat the slurry with an absorption dose of 5 kGy for 45 seconds, and then the pH was adjusted to neutral to obtain the pretreated slurry. (3) Add ethanol to the pretreated slurry, adjust the ethanol concentration to 45%, extract for 1.5 h, press and filter, and dry the filtrate to obtain rat worm extract 1.

[0019] 3. Preparation of Ratwort Extract 2: (1) Wash the whole plant of *Gnaphalium affine* and add twice the volume of water to grind it into a paste for later use; (2) Add ethanol to the pre-slurry, adjust the ethanol concentration to 45%, extract for 1.5 h, press and filter, and dry the filtrate to obtain rat worm extract 2.

[0020] 4. Preparation of Ratwort Extract 3: After washing the whole plant of *Gnaphalium affine*, add twice the volume of water and grind it into a paste. Then, heat it at 50°C for 1.5 hours, press and filter it, and dry the filtrate to obtain *Gnaphalium affine* extract 3.

[0021] Example 1: 1. Prepare different nutrient solutions according to the following formulas: Nutrient 1: 4g / L superphosphate + 3g / L borax + 500mg / L mouse thorn extract 1 + 70mg / L 5-aminolevulinic acid, add water to make up the balance.

[0022] Nutrient 2: 4g / L superphosphate + 3g / L borax + 500mg / L mouse thorn extract 2 + 70mg / L 5-aminolevulinic acid, add water to make up the balance.

[0023] Nutrient 3: 4g / L superphosphate + 3g / L borax + 500mg / L mouse thorn extract 3 + 70mg / L 5-aminolevulinic acid, add water to make up the balance.

[0024] Nutrient 4: 4g / L superphosphate + 3g / L borax + 70mg / L 5-aminolevulinic acid, add water to make up the balance; Nutrient 5: 4g / L superphosphate + 3g / L borax + 500mg / L mouse thorn extract 1, add water to make up the balance.

[0025] 2. Treatment of the cutting substrate: Treatment 1: After tilling the garden soil in the cutting site, spray with an 800-fold dilution of 50% carbendazim wettable powder (0.8 L / m²). 2 Then, pour in waste tobacco straw extract (1.5L / m2), cover with film for 3 days, then remove the film to prepare the cuttings.

[0026] Treatment 2: After tilling the garden soil in the cutting site, spray with an 800-fold dilution of 50% carbendazim wettable powder (0.8 L / m²). 2 Then pour in clean water (1.5L / m). 2 After covering with film for 3 days, remove the film and wait for the cuttings to be planted.

[0027] 3. Treatment of cuttings: Select 15-20cm of current-year semi-lignified or lignified Acanthopanax senticosus branches, retaining 2-3 buds. Soak the branches in a 900-fold dilution of 50% carbendazim wettable powder for 10 minutes, then rinse them thoroughly with clean water. Next, immerse the lower 2-3cm of the branches in a 250mg / L indoleacetic acid solution for 4 minutes. Remove the pretreated cuttings for later use (planting should be completed within 4 hours of removal). 4. Cuttings treatment: Plant the pretreated cuttings at a spacing of 4-5 cm between plants and 5-8 cm between rows, and water them thoroughly. Two days after planting, spray the substrate with a nutrient solution, and then spray with a 0.2% potassium dihydrogen phosphate solution every 15 days. Control the temperature at 18-25℃ and the relative humidity at 80%-90% throughout the entire planting period.

[0028] Multiple cutting experiments were set up using the above method, with different cutting substrate treatments and nutrient solutions. Cuttings were taken on February 26th. The specific cutting methods for each group are shown in Table 1 below: Table 1 After 30 days of observation, it was found that the mortality rate of the cuttings in experimental group 6 had exceeded 50% (blackening of buds and withering and decay of cuttings were considered signs of death). Therefore, no further statistics were collected on the growth of the cuttings in experimental group 6. This also confirmed to some extent that the use of waste tobacco straw extract alone would have an allelopathic effect on the Acanthopanax senticosus cuttings, affecting their rooting and survival.

[0029] Sixty days after cutting, the cuttings in each group were observed and statistically analyzed. The survival rate of the cuttings (dead cuttings were removed every 10 days during the cutting period), the average length and diameter of the new shoots, the average number of roots and the average root length (5 plants were randomly selected from each group and the average values ​​were calculated) were recorded. The specific results are shown in Table 2 below: Table 2 Experimental group 1 ( Figure 1 Experimental group 2 Figure 2 ) and experimental group 3 ( Figure 3 Overall, the cuttings grew well, but the new branches in group 2 were relatively thin and weak. Although the new branches in group 3 were the thickest, the number of roots was relatively small, which would affect the survival rate of subsequent transplanting.

[0030] Experimental group 4 ( Figure 4 Nutrient 4 used in the experimental group did not contain extracts of *Gnaphalium affine*, resulting in a higher overall mortality rate, and the surviving branches showed almost no sprouting, with only root growth; experimental group 5 ( Figure 5 Nutrient 5 used in group 7 did not contain 5-aminolevulinic acid, resulting in less root growth and the emergence of multiple branches from a single bud, but the elongation of each branch was poor, and they were basically unable to form a woody structure (branch diameter could not be measured); Figure 6 In the substrate treatment, no waste tobacco straw extract was added. The overall growth of the cuttings in group 1 was relatively weaker compared to group 1. This indicates that the method in group 1 effectively improved the growth of new shoots and root development of *Eleutherococcus senticosus* cuttings. Furthermore, the overall growth of the cuttings in group 1 was significantly better than that in group 2. Figure 7 As shown, the growth is basically uniform and the height of the new branches is relatively similar, indicating that the method in experimental group 1 has a high stability in its effect on Acanthopanax senticosus cuttings.

[0031] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for managing cuttings to promote the growth of Acanthopanax senticosus cuttings, characterized in that, The management method includes the following steps: S1. Preparation of cuttings: Cut semi-lignified or lignified branches from the current year, disinfect them, and soak them in rooting hormone to obtain pre-treated cuttings for later use. S2. Treatment of cutting substrate: Spray disinfectant on the cutting substrate and immerse it in waste flue-cured tobacco straw extract, then cover it with film for 2-3 days to obtain pretreated substrate. The waste flue-cured tobacco straw extract is an extract obtained by water extraction of waste flue-cured tobacco straw at a temperature below 30℃. S3. Cutting treatment: Insert the pre-treated cuttings into the substrate and water them thoroughly. After 2-3 days, spray the substrate at the lower end of the cuttings with a nutrient solution. The nutrient solution is formulated as follows: 3-5 g / L superphosphate, 2-4 g / L borax, 400-600 mg / L of *Gnaphalium affine* extract, and 40-100 mg / L of 5-aminolevulinic acid. S4. Daily management: Control the temperature and humidity appropriately, water in a timely manner, and apply foliar fertilizer every 15-20 days after cutting.

2. The cutting propagation management method according to claim 1, characterized in that: In step S1, the length of the pre-treated cuttings is 15-20cm, and 2-3 buds are retained on the pre-treated cuttings.

3. The cutting propagation management method according to claim 1, characterized in that: In step S1, the method of soaking the branches in rooting agent after disinfection is to soak them in a 50% carbendazim wettable powder solution diluted 800-1000 times for 10-12 minutes, rinse them with clean water, and then immerse the lower end in an indoleacetic acid solution with a mass concentration of 200-300 mg / L for 3-5 minutes.

4. The cutting management method according to claim 1, characterized in that: In step S2, the disinfectant sprayed is a 50% carbendazim wettable powder solution diluted 800-1000 times, and the dosage of the disinfectant sprayed is 0.8-1.2 L / m³. 2 Furthermore, the amount of waste tobacco straw extract added is 1-1.5 L / m³. 2 .

5. The cutting management method according to claim 1, characterized in that: The specific preparation method of the waste flue-cured tobacco straw extract in step S2 is as follows: after crushing the waste flue-cured tobacco straw, add water at a material-to-liquid ratio of 1:80-120, stir thoroughly, soak at a temperature below 30℃ for 2-3 days, and then press and filter. The filtrate is the waste flue-cured tobacco straw extract.

6. The cutting management method according to claim 1, characterized in that, The preparation method of the mouse thorn extract in step S3 includes the following steps: S3-1. Wash the whole plant of *Gnaphalium affine*, add water and grind it into a paste for later use; S3-2, Adjust the pH of the slurry to 5.5-6, then use... 60 The slurry was pretreated by Co-γ irradiation for 30-60 seconds, and then the pH was adjusted to neutral. S3-3. Add ethanol to the pretreated slurry, adjust the ethanol concentration to 40%-50%, extract for 1-1.5 hours, then press and filter, and dry the filtrate to obtain the extract of *Gnaphalium affine*.

7. The cutting management method according to claim 6, characterized in that: The absorbed dose of the irradiation treatment in step S3-2 is 3-6 kGy.

8. The cutting propagation management method according to claim 1, characterized in that: In step S4, the appropriate temperature and humidity are controlled by keeping the temperature between 18-25℃ and the relative humidity between 80%-90%.

9. The cutting management method according to claim 1, characterized in that: In step S4, foliar fertilization is performed by spraying with a 0.2% potassium dihydrogen phosphate solution.

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