A cultivation method for Stropharia rugosoannulata

Abstract

The application discloses a cultivation method for Stropharia rugosoannulata, which aims at irregular fruiting and dispersed yield, and comprises the following steps: culture material preparation: selecting Stropharia rugosoannulata cultivation raw materials to prepare culture materials, adding quicklime to adjust pH value, and then carrying out stacking fermentation, adjusting the water content of the culture materials to a suitable range after the fermentation is completed, and reserving; seeding and mycelium culturing: inoculating Stropharia rugosoannulata cultivation seeds into the prepared culture materials, controlling the thickness of the culture materials, and then placing the culture materials in a dark environment for constant-temperature mycelium culturing until the mycelium fully grows on the whole culture materials. The present application directly touches the growth development signal by supplementing exogenous adenosine, and does not need to wait for the slow accumulation of endogenous signal substances, so that the primordium formation time of Stropharia rugosoannulata can be advanced by 3-5 days compared with the conventional cultivation method, and the fruiting regularity is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of edible fungi cultivation technology, specifically to a cultivation method for *Stropharia macrocarpa* that results in uneven fruiting and dispersed yield. Background Technology

[0002] King cap mushroom, also known as wrinkled king cap mushroom, is a specialty edible mushroom variety promoted globally by the Food and Agriculture Organization of the United Nations. It has a crisp and tender texture, is rich in nutrients, including active polysaccharides, high-quality protein, and various minerals, and has both high edible and economic value. At the same time, king cap mushroom can be directly degraded and utilized from agricultural and forestry waste such as rice straw, corn cobs, and sawdust. After cultivation, the mushroom residue can be directly returned to the field to improve the soil. It is an important carrier for promoting the resource utilization of agricultural waste and building green circular agriculture. The cultivation mode is gradually upgrading from traditional open-field intercropping under forests to facility-based year-round cultivation, and the requirements for controllability of fruiting and standardization of production are constantly increasing.

[0003] Primordia formation is the core developmental node for edible fungi as they transition from vegetative to reproductive growth. It directly determines the fruiting time, uniformity of fruiting, and total yield, and is a key link in the precise management of facility cultivation. Currently, mainstream edible fungi such as oyster mushrooms and shiitake mushrooms have established mature environmental stress-induced primordia-inducing technology systems, which can achieve concentrated fruiting through temperature difference, light, and water supplementation. However, the developmental characteristics of giant king oyster mushrooms are unique, and they have low sensitivity to conventional primordia-inducing methods. Targeted and efficient primordia-inducing technology has always been lacking in production.

[0004] However, existing cultivation methods for *Stropharia macrocarpa* have the following drawbacks: First, primordia formation is slow, typically requiring 40-50 days after sowing to form primordia, resulting in a long single-crop cultivation cycle and low turnover efficiency of greenhouse facilities. Second, fruiting is uneven and yields are scattered, with primordia differentiation spanning 15-20 days, lacking a concentrated fruiting surge, requiring frequent and costly manual harvesting, and making it difficult to adapt to large-scale standardized production. Third, the bud-inducing effect is unstable, relying solely on indirect induction through environmental stresses such as temperature differences and light, making it highly susceptible to external climate fluctuations. Low temperature and low light conditions can easily lead to delayed fruiting and reduced yields, severely hindering the promotion of year-round greenhouse cultivation of *Stropharia macrocarpa*. Summary of the Invention

[0005] The purpose of this invention is to provide a cultivation method for *Stropharia macrocarpa* that addresses the problems of uneven fruiting and dispersed yield, thereby solving the problems of slow primordia development, poor fruiting uniformity, and unstable bud-inducing effect in existing *Stropharia macrocarpa* bud induction methods.

[0006] The technical solution of the present invention to solve the above-mentioned technical problems includes the following steps: S1 Culture Material Preparation: Select the raw materials for the cultivation of large-cap mushrooms to prepare the culture material. Add quicklime to adjust the pH value and then pile it for fermentation. After fermentation, adjust the moisture content of the culture material to a suitable range and set it aside. Alternatively, pure rice straw can be soaked in water and drained before being used as the culture material. S2 inoculation and mycelium growth: Inoculate the large-cap mushroom spawn into the prepared culture medium, control the thickness of the substrate, and place it in a dark environment for constant temperature mycelium growth until the mycelium completely covers the entire culture medium; S3 Covering Management: Evenly cover the surface of the culture medium covered with mycelium with sterilized covering material, level the surface and continue cultivation until the mycelium crawls into the covering layer and reaches 1 / 2 of the thickness of the covering layer. S4 Adenosine-induced budding: After entering the budding management stage, the top dressing method is to spray the soil layer with adenosine aqueous solution, or the base application method is to add adenosine during the mixing stage of the culture medium. The mycelium is induced to transform from vegetative growth to reproductive growth by supplementing adenosine from the outside. S5 bud-inducing environmental control: After applying adenosine, the air temperature, relative humidity, carbon dioxide concentration and light parameters of the cultivation environment are simultaneously regulated to provide suitable environmental conditions for primordium knot differentiation. S6 Fruiting Body Cultivation and Harvesting: After a large number of primordia have formed, adjust the environmental parameters to enter the fruiting body growth management stage. When the fruiting bodies grow to meet the commercial harvesting standards, harvest them in batches in a timely manner.

[0007] Preferably, in step S4, when applying the topdressing method during the bud induction period, adenosine is prepared into an aqueous solution and then evenly sprayed onto the surface of the casing layer using a misting or micro-spraying method. The spraying amount per square meter of cultivation area is 0.8–1.2 L. Mist / micro-spraying can ensure that the adenosine aqueous solution evenly covers the surface of the casing layer, avoiding the loss of the solution or uneven distribution caused by excessive water rinsing. This spraying amount can completely moisten the casing layer and allow slight seepage of water into the surface of the bottom culture medium, ensuring that the mycelia in the casing layer can fully contact and absorb adenosine, while preventing mycelial hypoxia due to water accumulation, and ensuring that the induction signal is evenly transmitted to the entire surface of the medium.

[0008] Preferably, the concentration of the adenosine aqueous solution used in the top dressing during the bud induction period in step S4 is 0.5–2 g / L, with the optimal application concentration being 1 g / L. When the adenosine concentration is below 0.5 g / L, the intracellular absorption is insufficient to exceed the reproductive and developmental threshold, resulting in a weak induction effect. When the concentration is above 2 g / L, the signal stimulation is too strong, which can easily lead to excessive hyphal growth and primordia malformation, and the marginal benefit decreases. 1 g / L is the optimal balance point between induction effect, fruiting body morphology, and application cost, which can simultaneously trigger hyphal differentiation and has the lowest malformation rate.

[0009] Preferably, when using the culture medium base application method in step S4, after the culture medium fermentation is completed in step S1 and before sowing, adenosine is dissolved and evenly mixed into the culture medium. During the fermentation process, the temperature of the material can reach above 60°C, which will cause the adenosine to decompose and become inactive. After the fermentation is completed, the temperature of the material drops. At this time, mixing in the culture medium can prevent the adenosine from being degraded by the high temperature. At the same time, the adenosine is evenly distributed in the culture medium by turning and mixing, so that the mycelium is continuously in contact with signal substances during the growth process. After the mycelium is established, the primordium differentiation is naturally and synchronously initiated.

[0010] Preferably, the culture medium in step S1 uses a pure rice straw formula or a mixed formula of 50% corn cob and 50% broadleaf sawdust; the composting and fermentation time is 7-10 days, during which the compost is turned 2-3 times; the adjusted moisture content of the culture medium is 60%-65%, and the pH value is 7.0-7.5; the carbon-nitrogen ratio of the above formula is suitable for the growth requirements of *Agaricus bisporus* mycelium, and can provide sufficient cellulose and hemicellulose nutrients; composting and fermentation can degrade coarse fiber, kill miscellaneous bacteria and insect eggs in the material, and improve the physicochemical properties of the culture medium; the 60%-65% moisture content takes into account the water requirements for mycelial growth and the permeability of the material layer, avoiding anaerobic decay; the neutral to slightly alkaline pH environment can inhibit the reproduction of miscellaneous bacteria, which is more suitable for the colonization and growth of *Agaricus bisporus* mycelium.

[0011] Preferably, in step S2, the sowing amount is 2% of the dry substrate mass, the substrate thickness is 15-20 cm, the mycelium growth environment temperature is controlled at 23-25℃, the carbon dioxide concentration is maintained at 3000-5000 ppm, and the mycelium growth period is 25-30 days. The 2% sowing amount ensures rapid colonization and sealing of the substrate by mycelium, reducing the risk of contamination by other microorganisms. The 15-20 cm substrate thickness provides both water and fertilizer retention capacity and air permeability, providing sufficient nutrients for subsequent fruiting. 23-25℃ is the optimal growth temperature for *Agaricus bisporus* mycelium, resulting in the fastest mycelial growth rate and strongest growth. High carbon dioxide concentration can inhibit premature primordia differentiation, ensuring that the mycelium focuses on vegetative growth. 25-30 days allows the mycelium to fully absorb the substrate, accumulating sufficient nutrients to support primordia differentiation and fruiting body growth.

[0012] Preferably, the casing material in step S3 is humus or peat moss, with a particle size of 0.5–1 cm, a casing thickness of 2–4 cm, and a casing moisture content of 20%–25%. Humus and peat moss have excellent water retention and air permeability, providing the physical stimulation and microbial environment required for primordia formation. A particle size of 0.5–1 cm maintains suitable porosity, balancing air permeability and water retention. A casing thickness of 2–4 cm ensures uniform mycelial growth; too thin a layer leads to water loss, while too thick a layer results in excessively deep primordia formation and an increase in deformed mushrooms. A moisture content of 20%–25% provides stable humidity for mycelial growth, promotes mycelial extension into the casing layer, and prepares for knotting.

[0013] Preferably, the specific environmental parameters for bud induction in step S5 are: air temperature 14-16℃, relative humidity 95%-99%, carbon dioxide concentration 1500-2500ppm, 10-12 hours of diffused light per day, and light intensity 300-800lx; the low temperature of 14-16℃ works synergistically with adenosine signal to jointly trigger the mycelium to transition from vegetative growth to reproductive growth; high humidity of over 95% can prevent the new primordia from dehydrating and wilting, ensuring normal primordia differentiation; appropriately reducing the carbon dioxide concentration can relieve the inhibition of vegetative growth and induce primordia formation; diffused light can promote primordia pigment deposition and normal differentiation. This light duration and intensity match the photoperiodic requirements for the development of Pleurotus ostreatus primordia, ensuring both the induction effect and avoiding strong light inhibiting primordia growth.

[0014] Preferably, in step S6, the relative humidity of the air during the fruiting body growth stage is adjusted to 90%–95%, and the temperature and carbon dioxide concentration are maintained consistent with those during the bud induction stage. The commercial harvesting standard is that the fruiting body stipe is 5–6 cm long, the mycelium is not ruptured, and the cap has not opened. Appropriately reducing humidity during the fruiting body growth stage can reduce the risk of contamination by miscellaneous fungi and rotting caused by high humidity. Stable temperature and carbon dioxide concentration can ensure uniform growth of the fruiting body and reduce the proportion of deformed mushrooms. Harvesting when the mycelium is not ruptured results in a crisp and tender fruiting body with the highest nutritional content, optimal commercial value, and shelf life. After the cap opens, the release of mycelial spores will lead to a rapid decline in quality and reduced marketability.

[0015] This invention has the following beneficial effects: it significantly shortens the primordium formation cycle and greatly improves the turnover efficiency of facility cultivation: by directly triggering reproductive development signals through exogenous adenosine supplementation, without waiting for the mycelium to slowly accumulate endogenous signal substances, the primordium formation time of *Agaricus macrocarpa* can be advanced by 3 to 5 days compared with conventional cultivation methods, and the uniformity of fruiting is greatly improved, shortening the overall cultivation cycle, effectively reducing facility depreciation and labor management costs, and significantly increasing the annual yield and annual income per unit area, providing core technical support for the year-round industrialized production of *Agaricus macrocarpa*.

[0016] Significantly improving fruiting uniformity and concentrating fruiting to reduce production and harvesting costs: By directionally activating the cAMP-PKA pathway within the mycelium, mycelia at different developmental stages synchronously initiate reproductive differentiation programs. The primordia differentiation time span can be compressed from the conventional 15-20 days to 5-7 days, increasing the primordia synchronization rate and forming a clear and concentrated fruiting flush. This significantly reduces the number of harvesting batches, lowering the frequency of manual harvesting and labor costs. Concentrated fruiting also promotes greater uniformity in fruiting body growth, resulting in more consistent grading of marketable mushrooms, facilitating standardized packaging and bulk sales. Furthermore, it lays the foundation for the subsequent application of mechanized harvesting equipment, adapting to the needs of large-scale, standardized industrial development.

[0017] The primordia-inducing effect is stable and reliable, with significantly enhanced resistance to external environmental fluctuations: Conventional environmental stress-based primordia-inducing methods are highly dependent on external conditions such as temperature differences and light intensity, and are greatly affected by seasonal changes, extreme weather, and the insulation and light transmission performance of greenhouses. In low-temperature and low-light seasons, problems such as delayed primordia formation, sparse primordia, or even no mushrooms may occur, resulting in a low success rate of primordia induction. This invention induces primordia formation by starting with the endogenous developmental pathway, with environmental factors playing only an auxiliary and synergistic role. The influence of external temperature, humidity, and light fluctuations is greatly reduced. Primordia formation can still be stably induced under unfavorable cultivation conditions such as low temperature and low light, greatly improving the success rate of primordia induction, effectively reducing production risks, ensuring the stable execution of the annual production plan, and meeting the precise production scheduling needs of contract farming.

[0018] The application method is flexible and simple, and can be directly adapted to existing cultivation and production systems: Two application schemes are provided: basal application to the substrate and topdressing during the bud induction period, to meet the needs of different production scenarios. Basal application to the substrate can be integrated into the regular mixing process, with a single addition providing full-effect, suitable for large-scale centralized material preparation. Topdressing during the bud induction period can be completed simultaneously with regular fruiting water operations, requiring no additional specialized equipment; it can be implemented using existing misting and micro-spraying systems, and the application rate can be flexibly adjusted for different mycelial growth stages, adapting to the needs of refined management. Both schemes are simple to operate and have low barriers to entry, allowing grassroots growers and production personnel to quickly master them without additional training. Furthermore, adenosine is a natural nucleoside substance, with no risk of residue after application, and it does not alter the nutritional components and flavor quality of the *Pleurotus ostreatus* fruiting bodies, meeting the production standards for green edible fungi. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall process flow of the cultivation method of the present invention.

[0020] Figure 2 This image shows the effect on day 10 after the application of adenosine solution (right) in this embodiment and after spraying water (left) in the control group. Detailed Implementation

[0021] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0022] See Figure 1 As shown, the technical solution adopted in this specific embodiment includes the following steps: S1 Culture Medium Preparation: Select the raw materials for the cultivation of large-cap mushrooms to prepare the culture medium. Add quicklime to adjust the pH value and then pile it for fermentation. After fermentation, adjust the moisture content of the culture medium to a suitable range and set it aside. The moisture content of the adjusted culture medium is 60% to 65% and the pH value is 7.0 to 7.5. Alternatively, pure rice straw can be used as the culture medium after soaking and draining. S2 Inoculation and Mycelium Growth: Inoculate the prepared culture medium with the spawn of *Agaricus bisporus*, control the thickness of the substrate, and place it in a dark environment for constant temperature mycelium growth until the mycelium completely covers the entire substrate. The inoculation amount is 2% of the dry substrate mass, and the substrate thickness is 15-20 cm. The temperature of the mycelium growth environment is controlled at 23-25℃, the carbon dioxide concentration is maintained at 3000-5000 ppm, and the mycelium growth period is 25-30 days.

[0023] S3 Casing Management: Evenly cover the surface of the culture medium covered with mycelium with sterilized casing material, level the surface and continue cultivation until the mycelium crawls into the casing layer and reaches 1 / 2 of the casing layer thickness. The casing material is humus or peat moss, with a particle size of 0.5-1cm and a casing thickness of 2-4cm. The moisture content of the casing is adjusted to 20%-25%.

[0024] S4 Adenosine-Induced Bud Induction: After entering the bud induction management stage, top-dressing with adenosine aqueous solution sprayed onto the soil layer, or basal application with adenosine added during the culture medium mixing stage, can induce mycelium to transform from vegetative growth to reproductive growth by supplementing with exogenous adenosine. When using the top-dressing method during the bud induction period, adenosine is prepared into an aqueous solution and then evenly sprayed onto the surface of the soil layer by misting or micro-spraying. The spraying amount per square meter of cultivation area is 0.8-1.2L, and the concentration of adenosine aqueous solution used for top-dressing during the bud induction period is 0.5-2g / L, with the optimal application concentration being 1g / L.

[0025] S5 bud-inducing environmental control: After applying adenosine, the air temperature, relative humidity, carbon dioxide concentration and light parameters of the cultivation environment are simultaneously regulated to provide suitable environmental conditions for primordium knot differentiation. The specific bud-inducing environmental parameters are: air temperature 14-16℃, relative humidity 95%-99%, carbon dioxide concentration 1500-2500ppm, 10-12 hours of diffused light per day, and light intensity 300-800lx.

[0026] S6 Fruiting Body Cultivation and Harvesting: After a large number of primordia have formed, adjust the environmental parameters to enter the fruiting body growth management stage. When the fruiting bodies grow to meet the commercial harvesting standards, harvest them in batches in a timely manner. During the fruiting body growth stage, adjust the relative humidity of the air to 90% to 95% and maintain the temperature and carbon dioxide concentration consistent with the bud induction stage. The commercial harvesting standard is that the fruiting body stipe is 5 to 6 cm long, the mycelium is not ruptured, and the cap has not opened.

[0027] The working principle of this invention is as follows: Spray with 1g / L adenosine aqueous solution during the bud induction period Culture medium preparation: Use pure rice straw formula, add water to pre-moisten to the moisture content to 65%, and set aside.

[0028] Sowing and mycelium growth: The spawn of *Agaricus bisporus* was inoculated using the layer sowing method, with a sowing amount of 2% of the dry substrate weight and a substrate thickness of 15cm. The substrate was placed in a dark incubation room for mycelium growth, with the temperature controlled at 24±1℃, relative humidity of 65%~70%, and carbon dioxide concentration of 3500~4500ppm. After 28 days of incubation, the mycelium had fully grown into the substrate.

[0029] Covering management: Select humus soil with a particle size of 0.5-1cm, disinfect it with hypochlorous acid, and adjust the moisture content to about 22%. Cover the soil evenly with about 3cm of soil, level it, and continue to cultivate for 7 days until the mycelium climbs to 1 / 2 the thickness of the covering layer.

[0030] Adenosine-induced budding: Prepare an adenosine aqueous solution with a concentration of 1 g / L, and spray it evenly on the surface of the casing layer using a mist sprayer at a rate of 1 L / m². Reduce ventilation for 24 hours after spraying and maintain a relative humidity of 98% to promote the absorption of adenosine by the mycelium.

[0031] Environmental control: After spraying, adjust the environmental parameters as follows: temperature 15±1℃, relative humidity 95%~98%, carbon dioxide concentration 1800~2200ppm, provide 11 hours of diffused light per day, and light intensity 400~600lx.

[0032] Harvesting and index determination: After the primordia form, adjust the relative humidity of the air to 90% to 93% and keep the temperature and carbon dioxide concentration constant; harvest when the fruiting body stipe is 5 to 6 cm long and the mycelium is not broken, and record the time of primordia appearance, the time span of primordia formation, the yield of the first flush of mushrooms, the biological conversion rate and the rate of deformed mushrooms.

[0033] like Figure 2 As shown in the figure, the results of this embodiment are as follows: Primordia began to appear on the 8th day after adenosine application, the concentrated formation time of primordia spanned 5 days, and the primordia synchronization rate was greatly improved.

[0034] Compared to conventional water-based bud induction The operation procedure was the same as spraying a 1g / L adenosine aqueous solution during the bud induction period, but an equal volume of water was sprayed during the bud induction period without adding adenosine. Results: Primordia began to appear on the 12th day after covering with soil, with a primordia formation time span of 18 days and a low primordia synchronization rate.

[0035] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A cultivation method for *Stropharia macrocarpa* that addresses uneven fruiting and dispersed yield, characterized in that... Includes the following steps: S1 Culture Medium Preparation: Select the raw materials for the cultivation of large-cap mushrooms to prepare the culture medium. Add quicklime to adjust the pH value and then pile it for fermentation. After fermentation, adjust the moisture content of the culture medium to a suitable range and set it aside. S2 inoculation and mycelium growth: Inoculate the large-cap mushroom spawn into the prepared culture medium, control the thickness of the substrate, and place it in a dark environment for constant temperature mycelium growth until the mycelium completely covers the entire culture medium; S3 Covering Management: Evenly cover the surface of the culture medium covered with mycelium with sterilized covering material, level the surface and continue cultivation until the mycelium crawls into the covering layer and reaches 1 / 2 of the thickness of the covering layer. S4 Adenosine-induced budding: After entering the budding management stage, the top dressing method is to spray the soil layer with adenosine aqueous solution, or the base application method is to add adenosine during the mixing stage of the culture medium. The mycelium is induced to transform from vegetative growth to reproductive growth by supplementing adenosine from the outside. S5 bud-inducing environmental control: After applying adenosine, the air temperature, relative humidity, carbon dioxide concentration and light parameters of the cultivation environment are simultaneously regulated to provide suitable environmental conditions for primordium knot differentiation. S6 Fruiting Body Cultivation and Harvesting: After a large number of primordia have formed, adjust the environmental parameters to enter the fruiting body growth management stage. When the fruiting bodies grow to meet the commercial harvesting standards, harvest them in batches in a timely manner.

2. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: When applying the top dressing during the bud-inducing stage in step S4, adenosine is prepared into an aqueous solution and then evenly sprayed onto the surface of the soil covering layer by misting or micro-spraying. The amount of spraying per square meter of cultivation area is 0.8 to 1.2 L.

3. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: In step S4, the concentration of adenosine aqueous solution used for topdressing during the bud-inducing period is 0.5–2 g / L, with the optimal application concentration being 1 g / L.

4. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: When using the culture medium base application method in step S4, after the culture medium fermentation is completed in step S1 and before sowing, adenosine is dissolved and evenly mixed into the culture medium.

5. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: In step S4, the amount of adenosine added to the culture medium is 10-50 mg / kg based on the dry culture medium mass, with the optimal addition amount being 30 mg / kg.

6. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: The culture medium in step S1 uses a pure rice straw formula or a mixed formula of 50% corn cob and 50% broadleaf sawdust; the fermentation time is 7 to 10 days, during which the pile is turned 2 to 3 times; the moisture content of the culture medium after adjustment is 60% to 65%, and the pH value is 7.0 to 7.

5.

7. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: In step S2, the seeding amount is 2% of the dry culture medium mass, the culture medium thickness is 15-20cm, the mycelium growth environment temperature is controlled at 23-25℃, the carbon dioxide concentration is maintained at 3000-5000ppm, and the mycelium growth culture time is 25-30 days.

8. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: In step S3, the covering material is selected from humus or peat moss, the particle size of the covering material is 0.5-1cm, the covering thickness is 2-4cm, and the moisture content of the covering material is adjusted to 60%-70%.

9. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: The specific environmental parameters for bud induction in step S5 are: air temperature 14-16℃, relative humidity 95%-99%, carbon dioxide concentration 1500-2500ppm, 10-12 hours of diffused light per day, and light intensity 300-800lx.

10. The cultivation method for *Stropharia macrocarpa* with uneven fruiting and dispersed yield according to claim 1, characterized in that: In step S6, the relative humidity of the air during the fruiting body growth stage is adjusted to 90%–95%, and the temperature and carbon dioxide concentration are maintained at the same level as during the bud induction stage. The commercial harvesting standard is that the fruiting body stipe is 5–6 cm long, the mycelium is not ruptured, and the cap has not opened.

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