A method for breeding tussah silkworms in an all-process waterless environment to prevent and control tussah silkworm streptococcosis

Abstract

The application discloses a cultivation method for precise water control and prevention of silkworm disease without water disaster, and belongs to the technical field of tussah silkworm cultivation. Tussah silkworms are prone to excessive feeding of high-water-content tender branches due to their water-loving nature, which leads to intestinal water imbalance. Streptococcus first damages the intestinal mucosa, inducing mixed infection of pyosis and microparticle disease. Existing cultivation water control is rough, and lacks targeted disease control means. The application constructs a closed-loop cultivation system: conducts pre-positioned germplasm screening, disinfects and air-dries the cultivation area, and removes virus-carrying individuals and high-humidity pathogenic conditions from the source. The branches are classified, high-water-content tender branches are independently water-cultivated, and are fed after being dried and adjusted by transpiration. The dry and wet states of silkworm sand are used as feedback basis to dynamically control the adjustment degree of the branches. The application does not require chemical bacteriostatic agents, retains trace dew on the leaf surface, completely eliminates water accumulation, optimizes the intestinal microecology of silkworms, blocks the infection channel of streptococcus, reduces the risk of secondary infection, and is suitable for multi-batch large-scale tussah silkworm cultivation.

Description

Technical Field

[0001] This invention relates to a method for controlling streptococcal disease in tussah silkworms in a water-free environment throughout the entire process, belonging to the field of intensive artificial breeding technology of tussah silkworms. Background Technology

[0002] tussah silkworm cocoons are a core raw material for silk processing and silkworm pupa deep processing industries. Tubers and willow branches are the main natural food sources for tussah silkworms. Indoor and greenhouse water-based centralized co-breeding models are gradually replacing the traditional pure wild-range breeding model due to their advantages of centralized management and uniform silkworm development. At present, conventional water-based silkworm rearing only relies on container immersion to maintain the basic freshness of tussah and willow branches and leaves, only addressing the surface needs of branches that dry out quickly and cannot be fed. The entire breeding system has several inherent shortcomings that are difficult for the industry to eradicate.

[0003] First, the existing disease prevention system focuses on the disinfection of the space and silkworms in the later stages of rearing, lacking a source control design. Even with regular disinfection of the rearing environment, the resident *Streptococcus tussah* bacteria carried by the silkworm eggs will proliferate in large numbers and induce disease in a continuously humid environment, indicating a significant loophole in the pre-emptive disease prevention design. The tussah silkworm itself has a simple nervous system and lacks a central mechanism for autonomously regulating its water intake. It has a natural tendency to feed on water, and will consume indiscriminately upon contact with fresh, tender oak leaves and willow leaves with high water content. Traditional rearing practices such as artificially spraying water onto branches and leaves to increase humidity, the presence of large amounts of stagnant water on branches, and the continuous flow of free water are the core causes of long-term high humidity. The trace amounts of natural morning dew on branches are extremely low in volume and will evaporate naturally in a short time, without causing excessive water intake by the silkworms or the harmful effects of environmental dampness. Long-term excessive water intake and a continuously humid environment can lead to water accumulation in the digestive tract, further amplifying the probability of streptococcal infection. After pathogens damage the peritrophic membrane and epithelial tissue of the midgut of the tussah silkworm, latent pustular viruses and microspores within the silkworm can invade the circulatory system through the damaged intestinal wall, easily causing a concentrated outbreak of complex diseases. Every spring and summer silkworm rearing cycle, large-scale disease outbreaks and reduced yields occur. Current control methods mostly involve passive remediation by spraying pesticides and spreading quicklime on the ground after the disease occurs. These methods can only eliminate pathogens in the environment and on the silkworm's body surface, but cannot repair the already damaged intestinal tissue. As a result, the disease recurrence rate is high, and the cost of disease prevention remains high.

[0004] Secondly, the lack of dynamic and adaptable branch conditioning techniques in current aquaculture is a core contributing factor to the high incidence of diseases. The fresh, tender shoots of oak and willow trees naturally have a high moisture content. When farmers directly harvest these high-moisture shoots for feeding, the stacking of shoots easily leads to heat and humidity buildup, causing leaf deterioration and contaminating the rearing environment. Silkworms, relying on their natural thirst, consume large quantities of these excessively moist leaves, resulting in water accumulation in their intestines, obesity, and a significant decrease in their immunity. Traditional open-air natural drying methods easily cause blockage and necrosis of the water-carrying vessels in the shoots, rendering them ineffective for water propagation and resulting in significant feed material loss and poor economic returns. The industry has long lacked a non-destructive conditioning solution for high-moisture shoots that can be adjusted according to the silkworm's metabolic state.

[0005] Third, there is no dynamic standard for judging the replacement of oak and willow branches. It relies entirely on the experience of the breeders to visually determine the progress of grazing and the degree of yellowing of leaves before replacing branches, lacking any reference to the breeding conditions. In high-temperature environments, the humidity around the water slots is high, and the remaining uneaten leaves are prone to fermentation and decay in the heat. This not only wastes the raw materials of branches, but the bacteria carried by the decaying leaves will continue to infect the silkworms, further increasing the probability of disease outbreaks.

[0006] Fourth, traditional silkworm rearing limits the suitable growth temperature to 20℃~28℃, requiring precise temperature control with heating and cooling equipment. The cost of equipment purchase and long-term electricity usage places a heavy burden on small-scale farmers in mountainous areas. In summer, outdoor temperatures can reach over 38℃. Even with greenhouses only having a PV film roof and open ventilation, the heat-concentrating effect of the PV film can further raise the internal temperature to 38~39℃. This combination of high temperature and sustained high humidity significantly increases the pathogenicity of *Streptococcus tussah*. There has been a lack of low-cost rearing and management solutions suitable for a wide temperature range of 8℃~35℃.

[0007] Fifth, the current prevention and control approach fragments the progressive infection relationship of the three types of diseases, which is a key reason for the unstable production of silkworms raised in concentrated co-breeding at the foot of the mountain. Disinfection of the site and equipment can only kill free-floating pustular viruses in the environment, but cannot eliminate latent virus particles and microspores in silkworm eggs and bodies. In low-density rearing in the wild, tussah silkworms feed on leaves with balanced moisture levels, making it difficult for streptococci to cause large-scale outbreaks. Pustular and microspore diseases only occur sporadically due to diseased germplasm. However, in high-density water-planted co-breeding environments, a fixed disease chain exists: excessive water accumulation in branches and leaves, and a continuously high-humidity environment promote the proliferation of streptococci, which breach the intestinal physical barrier. After intestinal damage, latent viruses invade the bloodstream and proliferate throughout the body. Microspores infect damaged epithelial cells via polar filaments, forming a mixed disease of bacterial disease followed by pustular and microspore diseases. Disinfection and lime application can only improve environmental humidity, but cannot repair the intestinal barrier or block the entire disease outbreak pathway. High-density co-breeding models are prone to disease year-round and have low survival rates.

[0008] Sixth, most existing silkworm rejuvenation technologies only target the rearing of 1st to 3rd instar silkworms, failing to cover the quality improvement needs of all age groups. In actual production, problems such as the decline in physical condition of young silkworms after being moved to the mountains, intestinal damage to older silkworms due to high-temperature environments, continuous accumulation of bacteria during the rearing cycle, and decreased immunity caused by environmental fluctuations are common. There is no robust rearing process suitable for mountain and forest rearing, indoor co-rearing with mutual rotation, and applicable to all age groups, making it impossible to achieve simultaneous rejuvenation and strengthening of silkworms at all ages.

[0009] Seventh, traditionally raised silkworm cocoons generally have a high moisture content. During the concentrated harvesting and stacking of fresh cocoons, the concentrated heat release from respiration makes them highly susceptible to mold growth on the pupae and blackening of the cocoon layers. The drying and management of fresh cocoons requires stringent control, involving a large workload for turning and ventilation, resulting in high material losses during storage and transportation. When fresh cocoons are sent to processing plants for drying, a large amount of free moisture needs to be removed, leading to high energy consumption and continuously increasing the cost of deep processing. Under conventional feeding conditions, cocoons raised in traditional spray-feeding and humid environments tend to have a high moisture content, while cocoons raised in arid regions naturally have a lower moisture content, thus narrowing the difference in moisture content between the two. Furthermore, the pure free-range model is highly constrained by weather and forest conditions, making it impossible to conduct planned multi-batch breeding according to production plans, thus compromising the stability of cocoon production.

[0010] Currently, most of the few publicly available water-propagation silkworm rearing techniques focus only on the prevention and control of single diseases. They lack a complete process system integrating the on-demand quality adjustment of oak and willow branches, environmental temperature control, real-time monitoring of silkworm excrement, and improvement of silkworm cocoon quality. This results in insufficient adaptability and makes it difficult to implement in extensive silkworm rearing scenarios in mountainous greenhouses. Conventional water-propagation structures simply replicate the water-soaking propagation method without matching dynamic water control, ventilation, and a complete set of management standards for daily inspections. Controlling diseases requires additional cooling and disinfection equipment, leading to high overall investment in the rearing process.

[0011] In summary, existing water-planting tussah silkworm farming only achieves basic preservation of tussah branches and willow branches, lacking a comprehensive pre-treatment system integrating the site, silkworms, and feeding branches and leaves. It also lacks methods for dynamic quality control and water management using live branches without damage, and cannot achieve physical disease prevention without chemical agents in a wide-temperature environment. Furthermore, it lacks a full-age silkworm rejuvenation program, dynamic judgment standards for branch replacement, and observable and verifiable farming indicators. In addition, it suffers from a series of shortcomings, including high losses during cocoon storage and transportation, high processing costs, and the inability to conduct orderly multi-batch production. The industry urgently needs a standardized tussah silkworm rearing process that has low barriers to entry for modification, low temperature control operating costs, dynamically quantifiable control indicators, and simultaneously addresses the triple prevention and control of streptococcal disease, septicemia, and microparticle disease in tussah silkworms. Summary of the Invention

[0012] The purpose of this invention is to provide a method for controlling streptococcal disease in tussah silkworms in a water-free environment throughout the entire process, in order to solve the problem that existing technologies lack non-destructive dynamic water quality control methods using live branches, and cannot achieve physical disease prevention without chemical agents.

[0013] To address the aforementioned problems, the present invention provides a method for controlling streptococcal disease in tussah silkworms in a water-free environment throughout the entire process, employing the following technical solution: A method for controlling streptococcal disease in tussah silkworms in a water-free environment throughout the entire process, comprising the following steps: (1) Pre-epidemic prevention and control stage ① Site basic disinfection and cleaning treatment: Before the silkworms are moved indoors or into the greenhouse, the breeding space, feeding racks, silkworm tables and all kinds of feeding utensils are thoroughly disinfected, cleaned and ventilated and dried. ② Silkworm selection and control: Purebred silkworm eggs that have passed microscopic examination and quarantine are selected and uniformly collected for breeding; healthy individuals of all ages of tussah silkworms transferred from the wild to the shed are selected by hand to be symmetrical, agile, free of disease spots, and uniform in growth and development and put into the breeding system. ③ Wide temperature range: Relying on the greenhouse shading facilities and the building's own natural heat insulation capacity, the temperature of the breeding environment can be stably maintained in the range of 8℃~35℃ by relying on the shading and enclosure structure. (2) Differentiated harvesting and external pre-drying and conditioning treatment of oak branches and willow branches The harvested oak and willow branches were divided into three categories: tender branches, moderately mature leaves, and mature leaves, and were then processed differently. Fresh and tender branches are placed in a separate water-insertion area outside the breeding area. The branches are allowed to air dry and condition themselves as needed through their own transpiration. This process maintains the normal physiological activity of the branches and adjusts them to a safe feeding range. Suitable and mature leaves should be selectively treated with disinfection, dust removal and impurity cleaning according to the actual needs of the site. After treatment, they can be used for feeding. During the harvesting, transportation, and shelving of oak and willow branches, it is essential to prevent them from being soaked by rainwater or from accumulating water on the branches, thus protecting them from large amounts of free water. When replacing branches, promptly remove any remaining leaves to prevent them from becoming moldy, breeding bacteria, and causing secondary pollution. (3) Daily feeding and rejuvenation and dry environment management The existing mature water propagation method was used to preserve and feed oak and willow branches; Clean the silkworm excrement regularly every day to keep the base of the silkworm rearing platform dry and loose. Use the dryness and wetness of the silkworm excrement as the sole basis for dynamic regulation: if the silkworm rearing platform is observed to be sticky, clumpy, or soft and wet, it is determined that the current moisture content of the branches and leaves is too high. Increase the degree of external pre-drying and conditioning for the branches to be harvested later, dynamically correct the feeding conditions, and continuously stabilize the water metabolism balance of the silkworm's intestines.

[0014] Furthermore, regular inspections are conducted throughout the entire breeding process to promptly identify and remove diseased silkworms and those that are weak or underdeveloped.

[0015] Furthermore, the temperature of the breeding area is stabilized by relying on shading and enclosure structures.

[0016] Furthermore, within the aforementioned breeding area, the leaves can be intermittently replenished with small amounts of water according to the dryness of the environment. This water replenishment only maintains the freshness of the leaves and does not produce any free water accumulation, thus not altering the core water-free breeding environment.

[0017] Furthermore, this method is applicable to the feeding and rearing of tussah silkworms at all ages from 1st to 5th instar, the rejuvenation of silkworm bodies, and standardized multi-batch cyclic breeding.

[0018] This invention controls tussah silkworm rearing and prevents diseases by controlling ambient temperature, maintaining feed using existing and mature water-based feeding methods, and controlling feed moisture content. It overcomes the existing misconception that physical disease prevention cannot be achieved without chemical agents. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly described below: Figure 1 This is a feeding flowchart according to an embodiment of the present invention. Detailed Implementation

[0020] To make the technical objectives, technical solutions, and beneficial effects of the present invention clearer, the technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only for explaining the present invention and are not intended to limit the present invention; that is, the described embodiments are merely some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0021] Specific embodiments of the tussah silkworm rearing method for controlling streptococcal disease in tussah silkworms in a water-free environment, as described in this invention, are as follows: Figure 1 As shown, this embodiment's process includes four main stages: S1 site disease prevention and silkworm environment adaptation, S2 differentiated harvesting and pre-drying of branches, S3 daily feeding and drying management, and S4 harvesting cycle. By regulating the moisture content of branches and controlling the humidity of the feeding environment, the intestinal water metabolism of tussah silkworms is stabilized, thus achieving the prevention and control of streptococcal disease in tussah silkworms. After harvesting, the silkworms can be recycled to the next batch of breeding processes.

[0022] Includes the following steps: (1) Pre-epidemic prevention and control stage ① Site basic disinfection and cleaning treatment: Before the silkworms are moved indoors or into the greenhouse, the breeding space, feeding racks, silkworm tables and all kinds of feeding utensils are thoroughly disinfected, cleaned and ventilated and dried. ② Silkworm selection and control: Purebred silkworm eggs that have passed microscopic examination and quarantine are selected and uniformly collected for breeding; healthy individuals of all ages of tussah silkworms transferred from the wild to the shed are selected by hand to be symmetrical, agile, free of disease spots, and uniform in growth and development and put into the breeding system. ③ Wide temperature range adaptability: Relying on the greenhouse shading facilities and the natural heat insulation capacity of the building itself, the temperature of the rearing environment can be stably maintained in the range of 8℃~35℃ by relying on the shading and enclosure structure; under extreme weather conditions, heating and cooling equipment can be used to assist in temperature control; when the ambient temperature is higher than 35℃, tussah silkworms are prone to heat stress; the higher the temperature and the longer the duration of high temperature, the more obvious the disorder of the intestinal physiological function and the decline of the body's immunity. Even if a water-free environment is achieved, streptococcal and secondary septic disease outbreaks can still be induced; different tussah silkworm varieties and different ages have different tolerance to high temperatures. Individuals may tolerate temperatures that are briefly above 35℃, but the risk of disease outbreaks increases significantly when exposed to environments above 35℃ for a long time.

[0023] (2) Differentiated harvesting and external pre-drying and conditioning treatment of oak branches and willow branches The harvested oak and willow branches were divided into three categories: tender branches, moderately mature leaves, and mature leaves, and were then processed differently. Fresh and tender branches are placed in a separate water-insertion area outside the breeding area. The branches are allowed to air dry and condition themselves as needed through their own transpiration. This process maintains the normal physiological activity of the branches and adjusts them to a safe feeding range. Suitable and mature leaves should be selectively treated with disinfection, dust removal, and impurity cleaning according to actual needs on site. After treatment, they can be used for feeding.

[0024] During the harvesting, transportation, and shelving of oak and willow branches, only large amounts of free water such as rainwater soaking and water flowing from the branches are prevented from entering the breeding area; the branches can carry a small amount of morning dew into the breeding area, and the small amount of dew will evaporate naturally and will not cause water accumulation. When replacing branches, promptly remove any remaining leaves to prevent them from becoming moldy, breeding bacteria, and causing secondary pollution. (3) Daily feeding and rejuvenation and dry environment management The existing mature water propagation method was used to preserve and feed oak and willow branches; Clean the silkworm excrement regularly every day to keep the base of the silkworm rearing platform dry and loose. Use the dryness and wetness of the silkworm excrement as the sole basis for dynamic regulation: if the silkworm rearing platform is observed to be sticky, clumpy, or soft and wet, it is determined that the current moisture content of the branches and leaves is too high. Increase the degree of external pre-drying and conditioning for the branches to be harvested later, dynamically correct the feeding conditions, and continuously stabilize the water metabolism balance of the silkworm's intestines.

[0025] Furthermore, regular inspections are conducted throughout the entire breeding process to promptly identify and remove diseased silkworms and those that are weak or underdeveloped.

[0026] Furthermore, within the breeding area, the leaves can be intermittently replenished with small amounts of water according to the dryness of the environment. The water replenishment only keeps the leaves fresh and does not produce any free water accumulation, thus not changing the core water-free breeding environment.

[0027] Furthermore, this method is applicable to the feeding and rearing of tussah silkworms at all stages from the 1st to the 5th instar, the rejuvenation of silkworm bodies, and standardized multi-batch cyclic breeding.

[0028] High-power, routinely operating forced temperature control equipment for heating and cooling is not an essential component of this method; daily cleaning of silkworm excrement is necessary to maintain a dry and loose state on the rearing platform, with the apparent dryness or wetness of the excrement serving as a daily monitoring indicator of the silkworms' health; dynamic regulation of branches and leaves is used to control the water intake of tussah silkworms, maintaining the integrity of their intestinal physiological structure, and achieving source control of streptococcus, septicemia, and microparticle disease in tussah silkworms. Disease control does not rely on spraying bactericidal and bacteriostatic chemical agents as the core means of disease prevention; during the rearing process, regular inspections and removal of diseased and weak silkworms can prevent the lateral spread of diseases.

[0029] Beneficial effects of the present invention 1. Construct a three-tiered, pre-emptive disinfection and closed-loop epidemic prevention system. Simultaneously implement three treatment processes: comprehensive disinfection of the entire breeding area, screening of healthy silkworm eggs / silkworm bodies, and disinfection of oak and willow branches. This prevents external bacteria and pathogens from invading the breeding environment. For the endogenous Streptococcus tussah carried by the silkworms themselves, physiological inhibition is achieved through a dry feeding environment. The entire epidemic prevention logic is closed-loop, stable, and reliable, suppressing the incidence of disease from both external and internal sources. Food-grade chlorine dioxide is used for disinfection, and its decomposition products only produce water and inorganic salts, which are non-toxic and leave no residue. The disinfection process does not damage the physiological functions of the silkworms, ensuring the accuracy and rigor of the data from the breeding control trials.

[0030] 2. Leveraging the inherent biological characteristic of tussah silkworms' preference for moist feed, this solution addresses the industry pain point of being unable to directly feed fresh, tender tussah and willow branches with high moisture content. It pioneers a live branch external water-insertion pre-drying and conditioning process. Tussah silkworms lack a central nervous system for regulating water intake and instinctively prefer to overeat tender branches and leaves with high moisture content, easily leading to water accumulation in the digestive tract and intestinal damage. Traditional open-air drying dehydration easily causes necrosis of the water transport vessels in the branches, losing their water-insertion preservation activity, resulting in a high rate of raw material waste. This solution relies on the transpiration of the live branches for gradual dehydration, and conditioning is performed as needed based on the actual dryness and moisture state of the silkworm excrement. There is no need to fixate on a 52%–66% moisture content range; mature leaves, mature tussah leaves, and willow leaves naturally match the feeding needs and can be directly fed. By controlling the silkworm's water intake from the source of feeding, it eliminates problems such as water accumulation, weakness, and decreased immunity in silkworms, significantly reducing the routine use of quicklime and disinfectants, and lowering the cost of breeding materials.

[0031] 3. It possesses excellent adaptability to a wide temperature range, with a safe rearing critical temperature of up to 35℃. A dry feeding environment cannot meet the conditions for the reproduction and proliferation of Streptococcus tussahii, making outbreaks of disease in large numbers unlikely under high temperatures. Temperature buffering and control can be achieved solely through the natural insulation of the greenhouse's shading netting and surrounding walls, eliminating the need for high-powered heating and cooling equipment. This makes it suitable for low-cost implementation by small-scale farmers, village cooperatives, and small-scale breeding bases in the mountainous areas of Songxian County, Henan Province. With only the top of the greenhouse covered by PV heat-retaining film and ventilation throughout, this system can still stably control disease problems even under stress conditions where the temperature inside the greenhouse rises to 38-39℃ in hot outdoor weather.

[0032] 4. Disease control is achieved entirely through physical methods, with no drug feeding and no drug residues. No antibacterial or therapeutic chemical agents need to be sprayed throughout the entire rearing cycle, resulting in no drug residues in cocoons and pupae, thus improving cocoon quality and the added value of deep processing of silkworm pupae. Staff determine the branch replacement time based on the appearance of the silkworm droppings and the leaf consumption, making the operation simple and easy for ordinary farmers to learn and implement.

[0033] 5. This technology enables the rejuvenation of silkworms throughout their 1st to 5th instar larvae, and is compatible with all mainstream tussah silkworm rearing methods on the market. Most existing conventional rearing methods are only applicable to the rearing of 1st to 3rd instar silkworms. This technology can complete intestinal repair, pathogen suppression, and body strengthening for all instar tussah silkworms. It is suitable for various practical production scenarios, including intensive indoor rearing, indoor rejuvenation followed by release into the mountains, quality improvement and correction of diseased and weak silkworms from the wild, stable production and disease prevention during high-temperature seasons, and staggered multi-batch breeding. The technology has comprehensive coverage. Rearing using this technology can steadily increase the number of individual cocoons and the proportion of high-quality cocoons, effectively reduce breeding losses caused by diseases, and improve overall breeding economic benefits.

[0034] 6. It can be directly upgraded based on existing commercially available water-feeding facilities, with low hardware modification costs; the whole process can support multiple batches of orderly ant breeding throughout the year, improving the utilization rate of breeding sheds and the total annual output.

[0035] 7. Significantly reduces the operating and maintenance costs of low-temperature control systems. The traditional suitable growth range for tussah silkworms is only 20℃~28℃. This process expands the safe rearing range to 8℃~35℃. In the extreme high-temperature outdoor environment in summer, the rearing environment can be controlled within a safe range by relying on shade nets. In winter, the low-temperature rearing requirements can be met by utilizing the heat storage structure of the greenhouse itself, saving the purchase of temperature control equipment, wiring, and continuous expenses incurred from long-term power consumption, which is in line with the current capital investment situation of grassroots farmers.

[0036] 8. The core innovation of this program lies in its integrated control of three diseases: streptococcal disease, pyorrhea, and microparticle disease, with the maintenance of intestinal physiological homeostasis as its core principle. Controlled water supply from branches and leaves stabilizes the integrity of the peritrophic membrane and epithelial cells in the midgut of the tussah silkworm. This directly inhibits the large-scale colonization and reproduction of Streptococcus tussahii, preventing bacterial softening disease. Furthermore, the intact intestinal barrier prevents ingested polyhedrosis viruses and microspores from invading the silkworm's blood and tissues, completely blocking the chain-like pathogenesis of "streptococcal damage to the intestine → secondary viral and microparticle infection." Increased nutrient absorption efficiency and enhanced immunity allow the silkworm's immune system to clear trace amounts of free pathogens from the environment, simultaneously reducing the incidence of pyorrhea and microparticle disease. This program abandons the traditional passive model of "treatment after disease onset," relying instead on environmental regulation combined with the silkworm's own physiological homeostasis to achieve synergistic control of three diseases. Compared to the traditional model of treating specific diseases individually, this approach offers lower control costs, more stable silkworm quality, and greater long-term operational reliability.

[0037] 9. Optimize the internal physicochemical quality of silkworm cocoons, reduce the moisture content of fresh cocoons, and improve the characteristics of the entire chain of silkworm cocoon drying, storage, transportation, and deep processing. Under the dynamic moisture control feeding system of this invention, the water metabolism of tussah silkworms is stable, with no excess free water accumulation in the body, and nutrients are fully deposited. The resulting cocoons have dense and thick cocoon layers, and regular and full cocoon shapes. Compared with traditional breeding methods, the dry matter content of the cocoons is higher and the commercial grade is better. Under conventional breeding conditions, the moisture content of the fresh cocoons produced by this scheme can be reduced by more than 8% compared with the spray water humidification feeding and humid outdoor rearing methods; the moisture content of tussah cocoons raised in arid environments is already low, and the difference in moisture content between the two will be correspondingly reduced. In the traditional high-moisture fresh cocoon stacking process, respiration heat release is concentrated, the probability of pupa mold and cocoon layer decay is high, the drying cycle is long, the workload of turning and managing the stacks is large, and the storage and transportation losses are high; at the same time, the deep processing of high-moisture silkworm cocoons has a large drying and dehydration load, and the processing energy consumption cost is high. The low-moisture silkworm cocoons produced by this process generate less heat when piled up, are less prone to spoilage during temporary storage at room temperature, improve drying efficiency, and significantly reduce material losses in all stages of harvesting, drying, storage, and transportation. It can also reduce the amount of dehydration in the factory drying process, save processing energy, and further increase the overall economic added value of silkworm cocoon products.

[0038] In summary, this invention breaks away from the long-standing industry practice of "supplementary watering and feeding, and treatment with medication for disease." By relying on a purely physical feeding and control method, it simultaneously achieves multiple technological benefits, including disease prevention and control, stable production and increased income, improved cocoon quality, reduced storage and transportation losses, and energy savings in processing. It effectively addresses a series of industry pain points, such as disease outbreaks in tussah silkworms during the rainy season, reduced production due to high temperatures and damaged sheds in summer, significant cocoon storage losses, and high deep processing costs. This invention possesses outstanding novelty, creativity, and practical value for industrialization.

[0039] The core disease prevention mechanism of this invention relies on dynamically adjusting the dryness and wetness of the feeding branches and leaves based on the state of the silkworm excrement. This stabilizes the intestinal physiological structure of the silkworm and enhances its inherent immunity. The silkworm's own immune system can then resist the infection and outbreak of streptococci, septicemia, and microparticle diseases. Multiple indoor controlled rearing experiments have confirmed that, under rearing conditions without any chemical disinfection, relying solely on the feeding system of pre-drying and conditioning the branches as needed, and blocking harmful free-floating water, can significantly reduce the incidence of disease while ensuring cocoon yield and cocoon quality. The trace amounts of morning dew allowed to enter the rearing environment by the branches will evaporate naturally within a short time, without causing dampness in the rearing environment and not constituting harmful water bodies that need to be blocked. Food-grade chlorine dioxide disinfection is only an optimized supplementary measure in intensive, large-scale centralized rearing scenarios to reduce the risk of exogenous bacteria invading the rearing system; it is an efficiency-enhancing operation and not a necessary technical condition for achieving the disease control effect of this invention.

[0040] Some farmers, when using this breeding system, will independently spread quicklime on the breeding platform for surface antibacterial and environmental cleaning. Quicklime can only inhibit surface bacteria on the breeding contact surface; it cannot repair damaged silkworm digestive tract tissues, nor can it fundamentally block the chain-like disease progression of streptococcal secondary pustular lesions and microparticle diseases. Therefore, it cannot achieve the disease control effect of this invention on its own. The perpetrator has completely replicated the core processes of this invention—branch moisture regulation, harmful water body barrier, and silkworm excrement health monitoring—adding only routine auxiliary operations such as quicklime application and simple environmental disinfection, and still falls within the scope of this patent protection.

[0041] This solution prohibits the continuous high-humidity breeding environment created by routine foliar spraying and overall greenhouse humidification in traditional silkworm farming. Minor, intermittent watering to improve leaf palatability during the breeding period does not alter the overall water management logic and is not intended to regulate silkworm survival humidity or prevent disease; such operations do not constitute modifications to the core technology of this solution. Furthermore, this system can stably maintain a suitable breeding temperature of 8℃~35℃ using shading structures and building envelope structures; high-power, routine heating and cooling units are not essential facilities. Users adding small cooling and heating auxiliary equipment only constitutes minor adjustments and optimizations to the breeding environment and does not change the underlying temperature control logic of this solution; such additional equipment does not constitute circumvention of this patent.

[0042] Compared to conventional humidified silkworm rearing, the moisture content of fresh cocoons raised using this dynamic moisture feeding model can be reduced by more than 8% under normal rainy and artificially irrigated conditions; the difference in moisture content between the two is correspondingly narrowed in arid natural rearing environments. The lower moisture content of the cocoons themselves reduces the likelihood of mold and spoilage during stacking, drying, and storage, thus reducing storage losses, increasing cocoon shell thickness and weight uniformity, and improving the commercial quality and storage stability of the cocoons.

[0043] Example 1: Grouped control experiment of monomorphic tussah silkworms native to Song County, Henan Province Experimental materials: On April 15, 2026, the first-generation tussah silkworms from Henan Yuda were selected and raised in an oak garden to the fourth instar stage. The silkworms in the entire oak garden carried natural background pathogens, and the risk of disease outbreak was high. Healthy fourth-instar tussah silkworms with symmetrical body shape, agile movement and no disease spots on their body surface were selected from the oak garden population as the test samples.

[0044] The baseline number of subjects in each of the three experimental groups was 200 individuals, and the natural temperature range of the rearing environment was 8℃ to 35℃. All oak branches used in each group were uniformly treated with food-grade chlorine dioxide (the oxidation decomposition products of chlorine dioxide are only water and trace amounts of inorganic halide, with no toxic residues and no accumulation on oak leaves or rearing platforms; this disinfectant only has an oxidative inactivation effect on pathogenic bacteria, polyhedroviruses, and microsporidia, and will not damage the body tissues of the oak silkworms, nor interfere with their normal growth, metabolism, and immune function; it only uniformly smooths out the initial bacterial population in each group, eliminating experimental errors caused by the disinfection operation, and ensuring that the control experimental data are accurate, rigorous, and reproducible).

[0045] Experimental group settings Group A: Free-range group Two hundred experimental tussah silkworms were released into a closed oak slope for natural rearing. No artificial watering, pesticide application, or humidity control was carried out throughout the process, allowing the larvae to develop into cocoons naturally in the wild. At the same time, more than 4,000 ordinary production tussah silkworms of the same batch were released into the same oak plantation as a reference sample for actual field production.

[0046] Group B: Control group raised under conventional mountain conditions Two hundred healthy tussah silkworms, whose development and growth were completely consistent with those in Group A, were selected and transferred to a greenhouse at the foot of the mountain for separate rearing. The tussah branches were fed directly without pre-drying or conditioning. During the rearing period, water was sprayed regularly to increase humidity according to the dryness and wetness of the leaves. Silkworm-specific disinfectants were sprayed regularly, and quicklime was spread on the ground to carry out disease prevention and control. Silkworm excrement was cleaned regularly every day, and sick and weak individuals were removed manually in a timely manner.

[0047] Group C: The waterless conditioning feeding experimental group of this invention Two hundred healthy tussah silkworms of the same origin and with consistent growth were selected and raised in the same isolated area of ​​a greenhouse, 2 meters away from the rearing area of ​​Group B, to eliminate experimental deviations caused by differences in the microenvironment within the greenhouse. Fresh tussah branches were placed on independent water-supported racks outside the rearing area, and air-drying and conditioning were carried out by the transpiration of the branches themselves. The condition was dynamically adjusted to a suitable feeding state according to the dryness and wetness of the silkworm excrement, without fixing or restricting the moisture content range. During the harvesting, transportation, and feeding of tussah branches, only rainwater, soaking water, and flowing free water were isolated. The branches could carry a small amount of morning dew into the greenhouse, which could evaporate naturally in a short time without affecting the rearing conditions. The frequency and management standards for cleaning silkworm excrement and inspecting and removing diseased silkworms were completely consistent with those of Group B.

[0048] Feeding process and experimental results In the early stages of rearing, the latent pathogen load in groups B and C was the same, the onset time of the disease and the number of diseased silkworms in the early stage were basically synchronized, and there was no significant difference in the number of surviving individuals in the short term.

[0049] As the feeding cycle progressed, Group B silkworms, which had been fed high-moisture oak leaves for an extended period, experienced water accumulation in their digestive tracts. This led to the proliferation of Streptococcus tussah in the midgut, which gradually damaged the peritrophic membrane and epithelial cells. With the intestinal barrier damaged, latent polyhedrosis viruses and microspores in the silkworms invaded the hemocoel and various tissues of the body through the intestinal wall gaps, causing the disease to spread continuously and the number of diseased silkworms to rise steadily.

[0050] Group C effectively controlled the moisture levels of branches and leaves to prevent water accumulation in the silkworm's intestines, thus maintaining the integrity and stability of the silkworm's intestinal structure and inhibiting the large-scale colonization and reproduction of Streptococcus tussahii. When a small number of individuals became ill, the spread of the disease could be stopped by manually removing the diseased silkworms. The overall health of the silkworm population gradually improved, and the gap between the health level of the silkworms and the control group continued to widen.

[0051] In the field group A, the combined effects of the rainy and humid spring weather and pathogens carried by the silkworm eggs led to a concentrated outbreak of bacterial softening disease, septicemia, and microparticle disease. The vast majority of the 200 experimental silkworms in this group died from the disease, with only 5 completing cocoon spinning. Of these, only 2 were viable healthy cocoons suitable for the photosensitive test. Simultaneously, over 4,000 silkworms of the same batch released into the field suffered severe disease losses, ultimately yielding only over 300 viable cocoons. After screening, only 153 qualified live cocoons remained for the one-stage-two-stage photosensitive test.

[0052] The final measured data on the number of cocoons produced, the number of live cocoons, and the quality of the cocoons in the two greenhouses at the foot of the mountain are as follows: 1) Control group B: The tussah silkworms were under long-term disease stress and had insufficient nutrient accumulation. In the end, only 26 tussah silkworms completed cocooning, and 14 live qualified cocoons met the photosensitive test standards. The cocoon layer was thin and the cocoon shape was not regular. The actual measured average dry cocoon weight was 6.8g.

[0053] 2) Experimental Group C: Diseases occurred sporadically and their spread could be effectively blocked. Ultimately, 54 cocoons were successfully spun, and 44 live healthy cocoons were suitable for the photosensitive test. Due to the early pathogen stress, the silkworms experienced some growth loss, but the intact intestinal structure ensured normal nutrient absorption. The cocoons were dense, uniform, and full, with an average dry cocoon weight of 7.3g. Both the cocoon quality and the weight of a single dry cocoon were better than those of the conventional feeding control group.

[0054] Throughout the entire rearing cycle, the excrement of Group C silkworms remained dry and loose, allowing for a direct distinction between the health status of the two groups of silkworms.

[0055] Summary of the experiment in this embodiment Under the same silkworm species and initial virus-carrying conditions, the wild-raised model suffered the most severe disease losses, leading to significant yield reductions in large-scale field farming. Conventional artificial rearing methods, which combine water replenishment with drug disinfection, can only temporarily suppress surface diseases. Considering the lack of a self-regulating water center in tussah silkworms and their natural preference for feeding on tender branches and leaves with high water content, traditional feeding methods continuously cause water accumulation in the silkworm's digestive tract, which is the root cause of intestinal damage and secondary diseases. Drugs cannot repair the damaged intestinal tissue, ultimately resulting in a low number of effective cocoons and poor cocoon quality.

[0056] This invention employs an external air-drying and conditioning process for oak branches combined with a feeding process that controls harmful free water. While it cannot eliminate latent pathogens already present inside the silkworm, it can stabilize the physiological barrier of the silkworm's intestines and break the chain path of disease development: "streptococcus destroys the intestines → viruses and microparticles cause secondary systemic infection." Under harsh breeding conditions with high disease incidence, it can steadily increase the number of effective cocoons and improve the quality of silkworm cocoons.

[0057] Meanwhile, sampling and testing in this embodiment confirm that the moisture content of fresh cocoons raised by this invention is more than 8% lower than that of conventional high-humidity rearing and free-range rearing models. The difference in moisture content between the two in arid rearing plots is correspondingly narrowed. The cocoons have less free water and a higher proportion of dry matter, making them less prone to heat accumulation and mold growth during harvesting and piling. The drying cycle is shorter and the storage and transportation stability is significantly better than that of traditionally raised cocoons. This effectively solves the industry pain points of multiple diseases, low yields, and difficulty in preserving cocoons in silkworm rearing under rainy and humid conditions in spring.

[0058] Example 2: Grouped Control Experiment of Yuda Tussah Silkworm in High-Temperature Environment in Songxian County, Henan Province Experimental materials: Local Henan-grown Yuda No. 1 tussah silkworms were selected. All silkworm eggs underwent uniform egg surface disinfection and microparticle microscopy screening, ensuring initial cleanliness and absence of obvious pathogens. On June 4, 2026, ants were collected uniformly on the hillside of Zhupo, and the young silkworms were uniformly reared and managed in the wild. Once the silkworms reached the stage of uniform second molt, healthy individuals with consistent body shape, normal activity, and no external symptoms were selected as the test samples for this experiment.

[0059] Both groups of silkworms initially numbered 200. The control group was raised in an open-air shed with ventilation on all four sides and a PV film covering the top with a shade net. Due to defects in the top shading facilities, and the obvious heat-gathering and heat-storing properties of the PV film, although the shed was not airtight, local heat accumulation still occurred. When the outdoor ambient temperature was 37°C, the stable ambient temperature inside the shed could reach 38-39°C, and the silkworms were under continuous high-temperature stress for a long time.

[0060] The experimental group was placed in an independent indoor breeding space, relying on the building's natural heat insulation buffer. The indoor temperature was controllable throughout the process and the maximum temperature did not exceed 35℃. The temperature control safety margin was sufficient, and the equipment operation and maintenance costs were low and the adaptability was strong.

[0061] All groups were fed oak branches and uniformly treated with food-grade chlorine dioxide (chlorine dioxide oxidation decomposition products are only water and trace amounts of inorganic halide, with no toxic residue accumulation, and it only has an inactivation effect on various pathogenic microorganisms, without damaging the body tissue of the oak silkworm or interfering with the normal growth and metabolism of the silkworm, and only uniformly smooths out the initial environmental bacterial source base of each group, eliminating the experimental deviation caused by the disinfection process, and ensuring that the control test data are true, rigorous and reproducible).

[0062] Experimental group settings Group A: Control group consisting of conventionally raised animals in a ventilated PV film greenhouse on a mountaintop. The tested silkworms were conventionally raised in a well-ventilated greenhouse with a shaded roof. The oak branches were not air-dried or treated to preserve the natural high moisture content of the leaves. During the rearing period, the leaves were regularly sprayed with water to increase humidity, and routine disease control measures were implemented, including daily application of silkworm medications and spreading quicklime on the ground. Silkworm excrement was cleaned daily, and weak or diseased individuals were manually removed. The greenhouse maintained a high-temperature stress environment of 38–39°C due to heat accumulation at the top.

[0063] Group B: Anhydrous conditioning indoor rearing experimental group of this invention Healthy tussah silkworms of the same developmental stage were selected from the same source and housed in a separate, temperature-controlled indoor area, with the indoor temperature never exceeding 35°C. Fresh tussah branches were uniformly pre-dried and conditioned in water outside the rearing area, and their condition was dynamically adjusted to a suitable feeding level based on the feedback from the moisture content of the silkworm excrement, eliminating the limitation of fixed moisture content values. During the harvesting, transportation, and feeding of tussah branches, only rainwater soaking and flowing water were prevented from entering the area, allowing the branches to carry a small amount of morning dew for feeding, which could evaporate quickly and naturally. The frequency of daily inspections, cleaning of silkworm excrement, and removal of diseased silkworms was exactly the same as that of the control group, ensuring the uniqueness of the experimental variables.

[0064] Feeding process and current status of phased field measurements Although the control group greenhouse had ventilation and air circulation, the combined effects of heat accumulation and insufficient shading from the PV film at the top resulted in a persistently high-temperature environment, with the indoor temperature consistently 1-2°C higher than the outdoor temperature. Combined with the traditional methods of foliar irrigation and feeding with high-humidity tender leaves, the tussah silkworms ingested excessive water over a long period, leading to osmotic pressure imbalances in their digestive tract. This directly induced a massive proliferation of Streptococcus tussahii, which continuously eroded and damaged the peritrophic membrane and epithelial barrier in the midgut.

[0065] The key aquaculture mechanism is formed in this stage: Once high-temperature stress has caused intestinal damage and intestinal barrier disruption in silkworms, any subsequent foliar watering, environmental dusting, or external pesticide application can only affect the surface and environment, failing to repair the already damaged intestinal structure. Drug control is therefore extremely ineffective. With the intestinal breach persisting, latent polyhedroviruses and microspores continue to invade the hemocoel and other tissues, leading to a continuous chain reaction of disease outbreaks and exacerbating silkworm mortality.

[0066] The experimental group utilized a constant indoor temperature environment to avoid extreme high-temperature stress. Simultaneously, it employed the dynamic conditioning technology of the present invention, which maintained the integrity of the silkworm's intestinal structure and the homeostasis of its microbial community over a long period. This suppressed the initial infection by streptococci at the source, completely breaking the entire disease chain of "high-temperature intestinal damage → bacterial colonization → secondary infection by viruses and microparticles." Throughout the experiment, only a very few weak silkworms appeared sporadically; after manual removal, no disease spread, and the silkworms exhibited uniform development and robust growth.

[0067] At present, all experimental silkworms have completed cocooning, and the pupae are still in the development stage. The final values ​​of the cycle, such as the live pupa rate and the weight of dry cocoons, have not yet been finalized. This time, only the number of formed wet cocoons and the apparent cocoon quality characteristics are counted.

[0068] The actual measured data at this stage are as follows: 1) Control group A: High temperature and humidity combined with drug ineffective control led to the continuous spread of the disease. 200 tussah silkworms were finally harvested and only 52 mature wet cocoons were produced. The cocoons were generally thin, uneven in size, and had many deformed cocoons, resulting in poor overall quality.

[0069] 2) Experimental Group B: The breeding environment was stable, the intestines were healthy and intact, and the disease was controllable. 178 mature wet cocoons were finally harvested from 200 tussah silkworms. The cocoons were full, thick, and uniform, and the overall quality was significantly better than that of the traditional control group.

[0070] Throughout the entire experimental period, the silkworm excrement in the experimental group remained dry and loose, allowing for a direct distinction between the physiological health differences between the two groups of silkworms.

[0071] Summary of the experiment in this embodiment In the ventilated greenhouse environment where outdoor temperatures reach 37°C in summer and the PV film at the top creates a sustained heat stress of 38-39°C inside, the traditional breeding model of spraying water for moisture retention followed by drug prevention has fundamental flaws. Driven by their instinct to feed on moist soil, tussah silkworms will ingest large amounts of leaves with high moisture content. This, combined with the high temperature environment, damages the silkworm's intestinal immune barrier. Subsequent external drug application and ground dust application cannot repair the already damaged intestinal barrier, rendering disease control essentially ineffective. Ultimately, this results in low cocoon yield, poor cocoon quality, and extremely high breeding risks.

[0072] This invention employs a technical solution of temperature zone control, dynamic on-demand quality adjustment of oak leaves, and control of harmful high-humidity environments to stably control the breeding temperature within the optimal range of 8–35℃, preserving the integrity of the silkworm's intestinal physiology and fundamentally blocking the chain outbreak pathways of three core diseases: bacterial, viral, and microparticle-related diseases. Even under extreme high-temperature breeding conditions in summer, it can still significantly increase the number of cocoons and significantly optimize the external quality of the cocoons.

[0073] Meanwhile, this embodiment further verifies that the feeding system of the present invention can reduce the moisture content of fresh cocoons by more than 8% compared with the traditional high temperature and high humidity breeding mode. Under drought conditions, this reduction will be reduced accordingly. The dry matter accumulation of cocoons is more compact, which greatly improves the stability of fresh cocoon stacking, drying and storage, reduces mold loss and subsequent processing energy consumption, and achieves the dual benefits of stable breeding and quality improvement and optimized cocoon storage and transportation.

[0074] Overall Summary of Implementation Examples This invention verifies the technical advantages of this process under high humidity pathogen stress in spring and extreme high temperature heat stress in summer through two sets of comparative experiments covering the core aquaculture conditions throughout the year. It fully covers three aquaculture systems: free-range farming, conventional drug-treated greenhouse farming, and dynamic water control intensive farming of this invention.

[0075] Traditional breeding methods rely on foliar feeding of branches and leaves to match the silkworm's natural preference for moist food. This results in the silkworm passively ingesting excessive water, which, combined with the high temperature and humidity, directly damages the silkworm's intestinal barrier, inducing primary streptococcal infection, followed by secondary diseases caused by viruses and microparticles. Relying on post-breeding medication and disinfection is a remedial measure that cannot solve the root problem. As a result, the breeding process is characterized by high losses, unstable yields, and inconsistent cocoon quality.

[0076] This invention completely abandons the traditional water-supplementation approach to silkworm rearing. It focuses on dynamic, on-demand quality adjustment, temperature control, and dry, healthy management to counteract the physiological drawbacks caused by the silkworm's preference for water. It addresses the root causes of disease, reduced yields, and unstable production in silkworm rearing, significantly improving cocoon setting rate, live cocoon rate, and cocoon quality. Simultaneously, through continuous low-water metabolism regulation, it achieves a stable reduction of over 8% in cocoon moisture content, thus reducing the impact of drought conditions and effectively solving the industry pain points of traditional silkworm cocoon storage, high losses during drying, and high energy consumption during processing.

[0077] This invention divides the basic core breeding process into several preferred supporting implementation schemes: graded water propagation and pre-drying of branches, dynamic control of branch and leaf moisture based on silkworm excrement status, prevention of harmful free water accumulation, control of breeding temperature range, and monitoring of silkworm health based on silkworm excrement status are essential basic technical features of this scheme; chlorine dioxide disinfection, no application of chemical disease prevention agents throughout the process, and passive temperature control using shade nets are all preferred supporting methods. Breeders can freely select various supporting methods according to different usage scenarios such as small-batch indoor breeding, joint breeding in the wild, and large-scale centralized breeding in greenhouses. All selected methods are implemented based on the core mechanism of water control and disease prevention of this invention and are all within the protection scope of this patent.

[0078] Finally, it should be noted that the above embodiments are only for illustration and not for limiting the technical solutions of the present invention. Any equivalent substitutions, modifications or partial substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for controlling streptococcal disease in tussah silkworms in a water-free environment throughout the entire process, characterized in that, Includes the following steps: (1) Pre-epidemic prevention and control stage ① Site basic disinfection and cleaning treatment: Before the silkworms are moved indoors or into the greenhouse, the breeding space, feeding racks, silkworm tables and all kinds of feeding utensils are thoroughly disinfected, cleaned and ventilated and dried. ② Silkworm selection and control: Purebred silkworm eggs that have passed microscopic examination and quarantine are selected and uniformly collected for breeding; healthy individuals of all ages of tussah silkworms transferred from the wild to the shed are selected by hand to be symmetrical, agile, free of disease spots, and uniform in growth and development and put into the breeding system. ③ Wide temperature range: Relying on the greenhouse shading facilities and the building's own natural heat insulation capacity, the temperature of the breeding environment can be stably maintained in the range of 8℃~35℃ by relying on the shading and enclosure structure. (2) Differentiated harvesting and external pre-drying and conditioning treatment of oak branches and willow branches The harvested oak and willow branches were divided into three categories: tender branches, moderately mature leaves, and mature leaves, and were then processed differently. Fresh and tender branches are placed in a separate water-insertion area outside the breeding area. The branches are allowed to air dry and condition themselves as needed through their own transpiration. This process maintains the normal physiological activity of the branches and adjusts them to a safe feeding range. Suitable and mature leaves should be selectively treated with disinfection, dust removal and impurity cleaning according to the actual needs of the site. After treatment, they can be used for feeding. During the harvesting, transportation, and shelving of oak and willow branches, it is essential to prevent them from being soaked by rainwater or from accumulating water on the branches, thus protecting them from large amounts of free water. When replacing branches, promptly remove any remaining leaves to prevent them from becoming moldy, breeding bacteria, and causing secondary pollution. (3) Daily feeding and rejuvenation and dry environment management The existing mature water propagation method was used to preserve and feed oak and willow branches; Clean the silkworm excrement regularly every day to keep the base of the silkworm rearing platform dry and loose. Use the dryness and wetness of the silkworm excrement as the sole basis for dynamic regulation: if the silkworm rearing platform is observed to be sticky, clumpy, or soft and wet, it is determined that the current moisture content of the branches and leaves is too high. Increase the degree of external pre-drying and conditioning for the branches to be harvested later, dynamically correct the feeding conditions, and continuously stabilize the water metabolism balance of the silkworm's intestines.

2. The method for controlling streptococcal disease in tussah silkworms in a water-free environment according to claim 1, characterized in that, Regular inspections are conducted throughout the entire breeding process to promptly identify and remove diseased silkworms and those that are weak or underdeveloped.

3. The method for controlling streptococcal disease in tussah silkworms in a water-free environment according to claim 2, characterized in that, The temperature of the breeding area is stabilized by relying on shading and enclosure structures.

4. The method for controlling streptococcal disease in tussah silkworms in a water-free environment according to claim 3, characterized in that, The leaves can be intermittently replenished with small amounts of water within the breeding area according to the dryness of the environment. The water replenishment only keeps the leaves fresh and does not produce any free water accumulation, thus not changing the core water-free breeding environment.

5. A method for controlling streptococcal disease in tussah silkworms in a water-free environment according to any one of claims 1-4, characterized in that: This method is applicable to the feeding and rearing of tussah silkworms at all stages from the 1st to the 5th instar, the rejuvenation of silkworms' physical condition, and the standardized breeding of multiple batches.

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