Rice anti-lodging agent, preparation method and application thereof

Abstract

The application belongs to the technical field of rice planting, and discloses a rice lodging-resistant agent and a preparation method and application thereof. The rice lodging-resistant agent comprises the following components in parts of mass: calcium topramezone 0.01-0.05 parts, potassium silicate 1-5 parts, trinexapac-ethyl 0.01-0.05 parts, boron-zinc fertilizer 0.5-2.0 parts, calcium ammonium nitrate 1-4 parts, organic potassium salt 0.5-2.0 parts and azoxystrobin 0.2-1.0 part. The rice lodging-resistant agent can improve the mechanical strength of stems and stalks by adjusting the internode length and the internode weight, reduce the lodging risk, and also can improve the yield of rice, and can adjust the development of rice through potassium source, further improve the lodging resistance and solve the sheaf wrapping phenomenon of rice.

Description

Technical Field

[0001] This invention belongs to the field of rice cultivation technology, and particularly relates to a rice lodging resistant agent, its preparation method and application. Background Technology

[0002] Lodging is a significant factor affecting rice yield and quality. Studies have shown that appropriately increasing plant height can increase biomass and thus rice yield. However, increasing plant height also increases the likelihood of lodging. How to appropriately increase biomass while minimizing lodging has become a challenge in rice cultivation research in recent years. Furthermore, different crops have different lodging factors, necessitating the development of a lodging-resistant agent specifically for rice to enhance its lodging resistance and increase yield.

[0003] Studies have found a general correlation between the morphological characteristics of the above-ground and underground parts of a plant and its lodging resistance. Coordinating the growth of the above-ground and underground parts of a crop can effectively reduce the lodging index and improve its lodging resistance. For the above-ground parts of rice, internode length and weight are key indicators affecting its lodging resistance. For the underground parts of rice, the root system is an important organ for anchoring the rice plant and absorbing water and nutrients; root vitality is a key indicator affecting its lodging resistance.

[0004] Potassium is an important metabolic regulator, playing a crucial role in the growth of both the underground and above-ground parts of plants. Its effects on the above-ground parts primarily manifest as increased photosynthetic capacity, enhanced accumulation of non-protein nitrogen in the stems, resulting in well-developed mechanical tissues, stronger stems, increased resistance to lodging, and improved yield and quality. Its effects on the underground parts (root system) include promoting root development, improving the plant's ability to acquire water and nutrients, and strengthening its anchoring and supporting functions. Furthermore, different types of potassium salts have different mechanisms of action on plants. Therefore, how to regulate the growth of both above-ground and underground parts of rice through potassium, coordinate their nutrient transport and transformation, improve their growth status, enhance crop stress resistance, and improve lodging resistance is an urgent problem to be solved.

[0005] In addition, while shortening the internode length is a common method to improve rice lodging resistance, it can also easily lead to panicle bud formation (or neck bud formation), affecting panicle development. Therefore, it is necessary to reduce panicle bud formation while improving rice lodging resistance. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a rice lodging resistance agent, its preparation method and application. The rice lodging resistance agent can improve the mechanical strength of the stem by adjusting the internode length and internode weight, reduce the risk of lodging, and at the same time improve rice yield. It can also regulate rice development through potassium source, further improve lodging resistance and solve the problem of rice panicle formation.

[0007] The technical solution of the present invention is as follows: A rice lodging resistant agent comprises the following components by weight: 0.01-0.05 parts of calcium cyclohexane, 1-5 parts of potassium silicate, 0.01-0.05 parts of anti-lodging ester, 0.5-2.0 parts of boron-zinc fertilizer, 1-4 parts of calcium ammonium nitrate, 0.5-2.0 parts of organic potassium salt, and 0.2-1.0 parts of azoxystrobin.

[0008] A rice lodging resistant agent, based on the dosage per acre, comprises the following components: 0.2-1.0g of calcium cyclohexane, 20-100g of potassium silicate, 0.2-1.0g of lodging resistant ester, 10-40g of boron-zinc fertilizer, 20-80g of calcium ammonium nitrate, 10-40g of organic potassium salt, and 4-20g of azoxystrobin, with the remainder being 20kg of water.

[0009] Preferably, the organic potassium salt includes potassium humate.

[0010] Preferably, the organic potassium salt further includes small molecule organic acid potassium, which includes any one or more of potassium oxalate, potassium glutamate, potassium formate, potassium acetate, and potassium propionate.

[0011] More preferably, the small molecule organic acid potassium is potassium oxalate.

[0012] Preferably, the mass ratio of potassium humate to potassium small molecule organic acid is 1:(0.1~0.3).

[0013] The present invention also provides a method for preparing a rice lodging-resistant agent, wherein the raw materials are mixed and stirred evenly according to the formula to obtain the rice lodging-resistant agent.

[0014] This invention also provides the application of a rice lodging-resistant agent in improving the lodging resistance of rice or increasing rice yield.

[0015] Preferably, the rice lodging-resistant agent is sprayed at the late tillering stage to the early jointing stage of rice.

[0016] Preferably, the rice lodging-resistant agent is diluted with water 80 to 200 times before spraying.

[0017] Technical effects of the present invention: 1. In the rice lodging-resistant agent of the present invention, calcium cyclohexane regulates the development of aboveground parts and roots, calcium strengthens the stem's resistance to bending, and comprehensively inhibits excessive stem growth and reduces ineffective tillering; potassium silicate can enhance cell wall stability, and potassium and silicon synergistically improve the mechanical strength of the stem; anti-lodging ester precisely controls internode elongation and inhibits apical growth; boron and zinc fertilizer promote nutrient transport and improve the lignification degree of stems; ammonium calcium nitrate supplements nitrogen and calcium to prevent nutrient deficiency; organic potassium salt (potassium humate) improves the soil environment and enhances the root system's grip on the soil; and pyraclostrobin fungicide reduces lodging caused by diseases (such as sheath blight and rice blast).

[0018] The above components, taken together, can improve the lodging resistance of rice and increase rice yield.

[0019] 2. The rice lodging-resistant agent of this invention can lower the plant's center of gravity by shortening the internode length (especially the lower internode length), thereby increasing the mechanical strength of the stem and reducing the risk of lodging. Furthermore, while shortening the internode length, the internode weight does not decrease; in fact, it significantly increases the internode weight.

[0020] 3. The lodging-resistant agent for rice of this invention optimizes the distribution of photosynthetic products, ensuring panicle development and grain filling. Furthermore, the lodging-resistant agent does not lead to a decrease in tillering-to-panicle rate; although the panicle length is slightly shortened, this is compensated for by a significantly increased grain density, and it does not negatively impact yield. This indicates that the lodging-resistant agent effectively increases rice yield by increasing the number of grains per panicle, improving the grain filling rate, and increasing the thousand-grain weight.

[0021] 4. Regarding early and late rice, early rice shows a more significant shortening of internode length and a higher internode weight, which is controlled within a certain range. The basal stem structure exhibits high strength and is relatively uniform, resulting in a more significant improvement in lodging resistance and yield.

[0022] 5. The rice lodging-resistant agent of this invention combines inorganic potassium salts with organic potassium salts, which can comprehensively regulate the rate of potassium absorption by rice, better promote the development of rice stem structure, and improve the lodging resistance of rice.

[0023] 6. The organic potassium salt of this invention includes both potassium humate (large molecule) and small molecule organic potassium acid, which can further improve lodging resistance and yield by enhancing rice root vigor. Among them, the addition of small molecule organic potassium acid has a more significant impact on late rice, with a more pronounced change in the lodging index of late rice, ultimately making the lodging index of late rice closer to that of early rice, thus significantly improving the lodging resistance of late rice.

[0024] In addition, when potassium oxalate is further selected from small molecule organic acid potassium, it can also solve the problem of panicle formation in early rice of the present invention, further improve the yield of early rice, and achieve a dual improvement in rice biomass and lodging resistance. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Unless otherwise specified in the following examples, the implementation conditions are generally those of routine experiments, and the raw materials are all commercially available or prepared by conventional methods in the art. Example 1

[0027] This embodiment provides a rice lodging resistance agent, comprising the following components. Each component is mixed thoroughly with 20 kg of water before spraying. The spraying concentration (mass concentration) of each component is shown in the table below: Example 2

[0028] This embodiment provides a rice lodging-resistant agent, which differs from Embodiment 1 in that the spraying concentration of each component is different.

[0029] Specifically, this embodiment includes the following components. After mixing each component with 20 kg of water until homogeneous, it can be sprayed. The spraying concentration (mass concentration) of each component is shown in the table below: Example 3

[0030] This embodiment provides a rice lodging-resistant agent, which differs from Embodiment 1 in that the spraying concentration of each component is different.

[0031] Specifically, this embodiment includes the following components. After mixing each component with 20 kg of water until homogeneous, it can be sprayed. The spraying concentration (mass concentration) of each component is shown in the table below: Example 4

[0032] This embodiment provides a rice lodging resistance agent, which differs from Example 1 in that the organic potassium salt includes potassium humate and potassium oxalate. Specifically, it includes the following components: Example 5

[0033] This embodiment provides a rice lodging resistant agent, which differs from Embodiment 4 in that potassium oxalate is replaced with potassium glutamate, while the rest is the same as Embodiment 4. Example 6

[0034] This embodiment provides a rice lodging resistant agent, which differs from Example 4 in that potassium oxalate is replaced with potassium formate, while the rest is the same as Example 4. Example 7

[0035] This embodiment provides a rice lodging resistant agent, which differs from Example 4 in that potassium oxalate is replaced with potassium acetate, while the rest is the same as Example 4.

[0036] Comparative Example 1 The difference between this comparative example and Example 1 is that calcium cyclohexane and anti-rebound ester are replaced with an equal amount of uniconazole, and it does not contain boron zinc fertilizer, calcium ammonium nitrate, or potassium humate.

[0037] Comparative Example 2 The difference between this comparative example and Example 1 is that the anti-rebound ester is replaced with an equal amount of calcium cyclohexane, potassium silicate is replaced with an equal amount of potassium humate, and boron-zinc fertilizer and calcium ammonium nitrate are not included.

[0038] Comparative Example 3 The difference between this comparative example and Example 1 is that calcium cyclohexane is replaced with an equal amount of anti-rebound ester, and it does not contain potassium silicate or potassium humate.

[0039] Comparative Example 4 The difference between this comparative example and Example 1 is that calcium cyclohexane is replaced with an equal amount of uniconazole.

[0040] Comparative Example 5 The difference between this comparative example and Example 1 is that calcium cyclohexane is replaced with an equal amount of anti-rebound ester.

[0041] Comparative Example 6 The difference between this comparative example and Example 1 is that the anti-reverse ester is replaced with an equal amount of calcium cyclohexanoate.

[0042] Comparative Example 7 The difference between this comparative example and Example 1 is that potassium silicate is replaced with an equal amount of potassium humate.

[0043] Comparative Example 8 The difference between this comparative example and Example 1 is that the rice lodging-resistant agent does not contain lodging-resistant esters.

[0044] Comparative Example 9 The difference between this comparative example and Example 1 is that the rice lodging-resistant agent does not contain boron-zinc fertilizer.

[0045] Comparative Example 10 The difference between this comparative example and Example 1 is that the rice lodging-resistant agent does not contain calcium ammonium nitrate.

[0046] Comparative Example 11 The difference between this comparative example and Example 1 is that potassium humate is replaced with an equal amount of potassium silicate.

[0047] Comparative Example 12 The difference between this comparative example and Example 1 is that the rice lodging resistant agent does not contain pyraclostrobin.

[0048] Application Example 1: Preliminary Exploration of Field Trials In the early stages of the experiment, the present invention selected four lodging-resistant agent formulations from Example 1 and Comparative Examples 1 to 3 to conduct experiments, and studied the effects of different lodging-resistant agent formulations on the lodging resistance (plant height, internode length, and internode weight of early and late rice) and yield of rice.

[0049] (a) Experimental Design The experiment was conducted from March to October 2025 in Guashe Township, Anfu County, based on a double-cropping rice planting pattern. The early and late rice varieties were Super Rice Zhongzao 35 and Qingxiangyou 19, respectively. Five treatments were included: a control group (CK) and lodging-resistant agent treatments (Examples 1 and 3). The lodging-resistant agent was sprayed at the late tillering stage to the early jointing stage of the rice. The CK was sprayed with the same amount of water, and other cultivation practices were the same. Each treatment was replicated three times, with a plot area of ​​16 m². 2 .

[0050] (II) Trial Management Early rice was sown on March 18 and transplanted on April 10, while late rice was sown on June 20 and transplanted on July 12. Transplanting was done by direct seeding. 24,000 seedlings were planted per mu for early rice and 20,000 seedlings per mu for late rice. Fertilization was carried out by deep application of base fertilizer and by spreading the fertilizer after shallow irrigation, allowing it to dissolve and infiltrate naturally. Specifically, for early rice, 7.5 kg of urea, 50 kg of calcium magnesium phosphate, 10 kg of potassium chloride, 250 kg of organic fertilizer, and 2.5 kg of zinc sulfate were applied per mu as base fertilizer. 7 days after transplanting, 7.5 kg of urea and 5 kg of potassium chloride were applied as tillering fertilizer. 25 days after transplanting, 5 kg of urea and 10 kg of potassium chloride were applied as panicle fertilizer. For late-season rice, apply 10 kg of urea, 50 kg of calcium magnesium phosphate fertilizer, 12.5 kg of potassium chloride, 300 kg of organic fertilizer, and 3 kg of zinc sulfate per mu as base fertilizer. Seven days after transplanting, apply 7.5 kg of urea and 5 kg of potassium chloride as tillering fertilizer. Twenty-five days after transplanting, apply 7.5 kg of urea and 12.5 kg of potassium chloride per mu as panicle fertilizer. Water management should adopt alternating dry and wet conditions, with wet irrigation as the main method. For pest and disease control, use new spraying equipment to improve the control effect.

[0051] (III) Test Results 1. Effects of lodging-resistant agents on plant height and internode length of early and late rice The plant height and internode length at the base of the stem (internode length) of rice in each group were measured, and the results are shown in Table 1. All lodging-resistant agent treatment groups showed reduced plant height and internode length, indicating that these agents effectively inhibited the longitudinal growth of early rice, resulting in shorter and stronger stems, thereby enhancing lodging resistance. Compared with the control (CK) and Comparative Examples 1-3, the early and late rice in Example 1 showed lower plant height and internode length, demonstrating a more pronounced lodging resistance effect. This indicates that the lodging-resistant agent of the present invention lowers the plant's center of gravity and increases the mechanical strength of the stem by shortening internodes (especially the lower internodes), which significantly reduces the risk of lodging under windy or rainy conditions.

[0052] Table 1. Effects of lodging-resistant agents on plant height and internode length of early and late rice.

[0053] 2. Effects of lodging-resistant agents on internode weight of early and late rice The weight per internode is a key indicator for measuring stem quality. The higher the value, the thicker and denser the internode is, the greater its mechanical strength and the stronger its resistance to breakage.

[0054] The internode weight of each group of rice was measured, and the results are shown in Table 2. Compared with CK and Comparative Examples 1 to 3, the internode weight of the first, second, and third internodes in Example 1 was consistently 0.22 g / cm, which was much higher than the corresponding value of CK, indicating that the lodging-resistant agent in Example 1 not only greatly shortened the internodes, but also showed that the basal stem structure was strong and relatively uniform.

[0055] Table 2 Effects of lodging-resistant agents on internode weight of early and late rice

[0056] 3. Effects of lodging-resistant agents on the lodging index of early and late rice During the late heading stage, the bending strength (g) of the basal internodes of rice was measured using a stem strength measuring instrument. The length from the basal internode to the top of the panicle and the fresh weight were also measured. The bending moment and lodging index of the rice stem were then calculated.

[0057] Bending moment (cm∙g) = Length from basal internode to ear tip (cm) × Fresh weight from basal internode to ear tip (g).

[0058] Lodging index [(cm∙g) / g] = (bending moment / flexural strength) ×100.

[0059] The lodging index results for each group are shown in Table 3. Compared with CK and Comparative Examples 1 to 3, the lodging index of each node of the early and late rice in Example 1 was lower, and the lodging resistance effect was more prominent.

[0060] Table 3 Effects of lodging-resistant agents on lodging index of early and late rice

[0061] 4. Effects of lodging-resistant agents on the yield and components of early and late rice Rice yield was calculated separately in each plot at maturity. Five representative plants were selected from each treatment group to measure the number of panicles per plant, number of grains per panicle, thousand-grain weight, and seed setting rate in different treatment groups to study the components of rice yield. The yield components and specific yield results of rice in each treatment group are shown in Tables 4 and 5, respectively.

[0062] As shown in Table 4, with the optimization of the formulation (from Comparative Example 1 to Comparative Example 3, and then to Example 1), the number of grains per ear and the grain density showed an increasing trend, with Example 1 showing the highest values. The thousand-grain weight of each lodging-resistant agent treatment group was higher than that of the control (CK), and the order was Example 1 > Comparative Example 3 > Comparative Example 2 > Comparative Example 1.

[0063] Table 5 shows that the yields of both early and late rice treated with the lodging resistance agent were higher than those in the control (CK), with Example 1 showing the best yield increase. This indicates that the lodging resistance treatment optimized the distribution of photosynthetic products, ensuring panicle development and grain filling. Furthermore, the lodging resistance agent did not lead to a decrease in tillering-to-panicle ratio; although the panicle length was slightly shortened, this was compensated for by the significantly increased grain density, thus not negatively impacting yield. This demonstrates that the lodging resistance agent effectively increased rice yield by increasing the number of grains per panicle, improving the seed setting rate, and increasing the thousand-grain weight.

[0064] Table 4. Yield components of different lodging-resistant agents for early and late rice

[0065] Table 5. Yield of different lodging-resistant agents for early and late rice (kg / mu)

[0066] (iv) Conclusion From the performance of the four lodging-resistant agents (Example 1, Comparative Examples 1 to 3) on lodging resistance and yield in early and late rice, Example 1 showed lower internode length, plant height, and lodging index, higher internode weight, and better yield, demonstrating more prominent lodging resistance and yield-increasing effects. Furthermore, the lodging resistance and yield-increasing effects of early rice were significantly better than those of late rice. These results indicate that the lodging-resistant agent of Example 1 can be considered a superior formulation, and further research should be conducted based on this formulation.

[0067] Meanwhile, as can be seen from Tables 1 and 3, the plant height control during the early rice growth period in Example 1 was too strong, causing some plants to have panicle buds. The panicle bud phenomenon in rice can easily affect its yield, which is also a problem that needs to be solved in the future.

[0068] Application Example 2: Effect of the components of the anti-lodging agent of the present invention Based on Application Example 1, this application example takes early rice as an example to further study the effects of each raw material component in the lodging-resistant agent formulation of Example 1 on the lodging resistance of rice (lodging index of the first internode of the key basal part).

[0069] The experimental design and management of this application example are the same as those of Application Example 1, except that 11 treatments were set up, namely the blank treatment group (CK) and the lodging-resistant agent treatments of Example 1, Comparative Examples 4 to 12. The lodging index of the first internode at the base of the rice in each group was calculated and statistically analyzed, and the results are shown in Table 6.

[0070] As shown in Table 6, compared with CK and Comparative Examples 4 to 12, the lodging index of rice treated with the lodging-resistant agent in Example 1 of the present invention was lower and the lodging resistance effect was better.

[0071] Table 6. Effects of different lodging-resistant agents on the lodging index of early rice.

[0072] Application Example 3: The Influence of Potassium Salt Composition in Lodging Resistance Agents Application Example 2 shows that potassium salts have a significant impact on the lodging resistance of rice. In this application example, the effects of potassium salt composition in the lodging resistance agent on rice root activity, lodging resistance (lodging index of the first internode at the critical base), and yield were studied by changing the potassium salt composition (adding different small-molecule organic potassium acids). The lodging index change rate was measured as the rate of change of each example group relative to the control group.

[0073] The experimental design and management of this application example are the same as those of Application Example 1, except that six treatments were set up: a blank treatment group (CK) and lodging-resistant agent treatments in Examples 1, 4 to 7. The lodging index of the first internode at the base of the rice plant and the rice yield were calculated and statistically analyzed for each group. The root activity of the rice plant was measured using the plant root activity test (TTC method), and the results are shown in Tables 7 and 8.

[0074] As shown in Tables 7 and 8, compared with Example 1, after adding small molecule organic acid potassium to the lodging-resistant agents of Examples 4 to 7 of the present invention to treat rice, the lodging index of early and late rice was further reduced, and the root activity and yield were also increased. This indicates that small molecule organic acid potassium can further improve lodging resistance and yield by increasing the root activity of rice.

[0075] Comparing the early and late rice in Examples 1 and 4, it can be seen that the addition of potassium small molecule organic acid has a more significant effect on late rice. The lodging index of early rice changed from -33.33% to -36.60%, while the lodging index of late rice changed from -25.02% to -40.54%. Ultimately, the lodging index of late rice became closer to that of early rice, significantly improving the lodging resistance of late rice.

[0076] Comparing the early and late rice cases in Examples 4 to 7, it can be seen that different small-molecule organic potassium acids have significantly different effects on root activity, lodging index, and yield. Example 4 showed superior lodging index, root activity, and yield. This indicates that potassium oxalate improves lodging resistance and yield by significantly enhancing rice root activity.

[0077] Table 7. Effects of different potassium salt compositions of lodging-resistant agents on root activity and lodging index of early and late rice.

[0078] Table 8. Effects of different potassium salt compositions of lodging-resistant agents on the yield of early and late rice (kg / mu)

[0079] Furthermore, in the early rice of this application example, it was found that some plants in Examples 1 and 5-7 still exhibited panicle budding, while in Example 4, panicle budding was basically absent (see Table 9 for details). This indicates that the addition of potassium oxalate further regulated the growth and development of the rice panicle, resolving the panicle budding phenomenon in early rice. This may also be one of the reasons for the significant increase in rice yield in Example 4.

[0080] Table 9. Effects of different lodging-resistant agents on the panicle formation of early rice.

[0081] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A rice lodging-resistant agent, characterized in that: The product comprises the following components by weight: 0.01-0.05 parts of calcium cyclohexane, 1-5 parts of potassium silicate, 0.01-0.05 parts of anti-rot ester, 0.5-2.0 parts of boron-zinc fertilizer, 1-4 parts of calcium ammonium nitrate, 0.5-2.0 parts of organic potassium salt, and 0.2-1.0 parts of azoxystrobin. The organic potassium salt includes potassium humate and small molecule organic potassium acid, wherein the small molecule organic potassium acid includes any one or more of potassium oxalate, potassium glutamate, potassium formate, potassium acetate, and potassium propionate.

2. A rice lodging-resistant agent, characterized in that: The dosage per acre includes the following components: 0.2-1.0g of calcium cyclohexane, 20-100g of potassium silicate, 0.2-1.0g of anti-rotting ester, 10-40g of boron-zinc fertilizer, 20-80g of calcium ammonium nitrate, 10-40g of organic potassium salt, and 4-20g of azoxystrobin, with the remainder being 20kg of water; The organic potassium salt includes potassium humate and small molecule organic potassium acid, wherein the small molecule organic potassium acid includes any one or more of potassium oxalate, potassium glutamate, potassium formate, potassium acetate, and potassium propionate.

3. The rice lodging-resistant agent according to claim 1 or 2, characterized in that: The mass ratio of potassium humate to potassium small molecule organic acid is 1:(0.1~0.3).

4. The rice lodging-resistant agent according to claim 1 or 2, characterized in that: The small molecule organic acid potassium is potassium oxalate.

5. A method for preparing the rice lodging-resistant agent as described in claim 1 or 2, characterized in that: Mix and stir all the ingredients according to the formula to obtain the rice lodging resistance agent.

6. The application of a rice lodging-resistant agent as described in claim 1 or 2 in improving the lodging resistance of rice and / or increasing rice yield.

7. The application according to claim 6, characterized in that: The rice lodging-resistant agent is sprayed at the end of the tillering stage to the beginning of the jointing stage of rice.

8. The application according to claim 6, characterized in that: Dilute the rice lodging-resistant agent with water 80 to 200 times and then spray it.

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