Application of high-efficiency haloxyfop- r-methyl in tobacco seedling microalgae prevention and control and prevention and control method
Patent Information
- Application Number
- CN202611171727.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-08-04
- Publication Date
- 2026-09-25
AI Technical Summary
然而，关于高效氟吡甲禾灵制剂作用于藻类细胞、用于水体微藻防控方面的应用，目前尚未见相关报道，将其应用于烟草育苗期微藻防控更未见公开
[0012]本发明与现有技术相比，具有明显的有益效果，从以上技术方案可知：本发明将市售的高效氟吡甲禾灵乳油用清水稀释后，按10～50 mg/L的有效浓度直接施入育苗池水体，可有效抑制水体及育苗盘表面微藻的滋生与繁殖，减少藻膜、藻垢的形成，改善育苗池水质，降低因微藻滋生引发的烟苗根系受损与病害风险。本发明所用药剂为市售常规高效氟吡甲禾灵乳油制剂，无需另行配制母液，配制方法简便、用量小、成本低、来源稳定；经活体试验证明，该浓度范围内对烟草种子的萌发率、烟苗根系生长及地上部生长均无不良影响，烟苗素质未受影响，对烟苗安全性好，且无需改变现有漂浮育苗的水肥管理流程。本发明采用高效、安全、环境友好、且来源稳定易于获取的市售制剂，能够在烟草育苗池中直接稀释施用，有效抑制育苗池水体及育苗盘表面微藻的滋生，同时对烟草种子萌发及烟苗生长安全，对于提高漂浮育苗烟苗素质、保障烟草生产安全具有重要意义，也是烟草产业绿色可持续发展的现实需要。
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Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural biotechnology, specifically to the application of a highly efficient flupyridine in the control of microalgae in tobacco seedlings, and also to a method for controlling microalgae in tobacco seedlings using a highly efficient flupyridine. Background Technology
[0002] Floating seedling cultivation is a widely adopted large-scale and professional seedling cultivation method in current flue-cured tobacco production. It has advantages such as uniform emergence, high seedling quality, and ease of intensive management. In this method, seedlings are cultivated by filling seedling ponds with water and floating seedling trays on the water surface. The water in the seedling ponds remains in a static or low-flow state throughout the entire seedling cycle, and the water contains a certain concentration of nutrients such as nitrogen and phosphorus. The suitable temperature and light conditions easily induce the proliferation of microalgae such as green algae and cyanobacteria.
[0003] Once microalgae proliferate on the surface of seedling ponds and trays, they form algal films or scale on the water and substrate surfaces, causing a decrease in dissolved oxygen and abnormal fluctuations in pH levels. They also compete with tobacco seedling roots for water and nutrients. The decomposition of algal residues can also breed pathogenic microorganisms, inducing diseases in tobacco seedlings, leading to poor root development, yellowing leaves, and weakened growth. In severe cases, this can cause a decline in the overall quality of the seedlings, ultimately affecting the growth of transplanted tobacco plants and the yield and quality of tobacco leaves. Microalgae growth is characterized by its wide occurrence, rapid reproduction, and high recurrence rate, making it a common and pressing problem in floating seedling production.
[0004] Currently, the main control measures for microalgae growth in seedling beds include strengthening water and fertilizer management, controlling phosphate fertilizer usage, disinfecting seedling trays, laying black film for shading, and treating with copper sulfate solution. While copper sulfate has a certain inhibitory effect on microalgae, its concentration range is narrow, and improper use can easily toxicize tobacco seedling roots. Furthermore, copper ions tend to accumulate in the water and substrate of the seedling bed, posing certain environmental and residue risks. Long-term use may also adversely affect subsequent tobacco seedlings and soil. Yao Yuanyuan's research ("Research on Algae Control Technology in Tobacco Seedbeds," Master's Thesis, Shandong Agricultural University, 2011) shows that while potassium permanganate and mancozeb have some inhibitory effect on Oscillatoria and Navicula, the application concentration is high (only 1000 mg / L can inhibit more than 95% of Oscillatoria), and their safety for tobacco seedlings needs further verification. In summary, there is currently a lack of a simple, effective, safe, and environmentally friendly pesticide application scheme for the control of microalgae during the tobacco seedling stage.
[0005] Haloxyfop-P-methyl, belonging to the aryloxyphenoxypropionate class of compounds, is the highly active form of haloxyfop-P-methyl. Commercially available formulations are primarily 108 g / L haloxyfop-P-methyl emulsifiable concentrates, commonly used as selective herbicides in broadleaf crop fields to control gramineous weeds. Its mechanism of action involves inhibiting acetyl-CoA carboxylase (ACCase) in target plants, thereby inhibiting fatty acid biosynthesis. This formulation is highly safe for dicotyledonous plants, maintains relatively stable efficacy under low-temperature conditions, and is a commercially available, routinely registered product with a stable and readily available source. However, there are currently no reports on the application of haloxyfop-P-methyl formulations in controlling microalgae in aquatic bodies, and its application in controlling microalgae during tobacco seedling stages is not publicly available. Summary of the Invention
[0006] The purpose of this invention is to overcome the above-mentioned shortcomings and provide a highly efficient flupyridine haloxyfop-R-methyl application in the control of microalgae in tobacco seedlings. This application is effective in controlling the growth of microalgae in seedling ponds and trays during the tobacco seedling stage, has good control effect, is safe for tobacco seedlings, and is easy to use.
[0007] Another objective of this invention is to provide a method for controlling microalgae in tobacco seedlings using highly efficient flupyridine.
[0008] The present invention relates to the application of highly efficient flupyridine in the control of microalgae in tobacco seedling cultivation, wherein: the solution of highly efficient flupyridine preparation is sprayed or added to the tobacco seedling tray or seedling pond, so that the effective concentration of highly efficient flupyridine in the water of the seedling pond is 10-50 mg / L.
[0009] The present invention discloses a method for controlling microalgae in tobacco seedlings using highly efficient flupyridine, comprising the following steps:
[0010] (1) Preparation of drug solution: Take high-efficiency flupyradifurone emulsifiable concentrate, mix it with water to prepare a drug solution with a concentration of 100-500 mg / L, stir and mix well, and set aside for use;
[0011] (2) Before the large cross stage of tobacco floating seedling raising, add the drug solution prepared in step (1) to the water body of the seedling pond to make the concentration of high efficiency flupyridine haloxyfop-R-methyl 10-50 mg / L. After the drug is applied, the water and fertilizer management of the seedling pond is carried out normally.
[0012] Compared with existing technologies, this invention has significant beneficial effects. As can be seen from the above technical solution, this invention dilutes commercially available high-efficiency flupyradifurone emulsifiable concentrate with water and applies it directly to the seedling pond water at an effective concentration of 10-50 mg / L. This effectively inhibits the growth and reproduction of microalgae in the water and on the surface of the seedling trays, reduces the formation of algal films and scale, improves the water quality of the seedling pond, and reduces the risk of root damage and disease in tobacco seedlings caused by microalgae growth. The agent used in this invention is a commercially available conventional high-efficiency flupyradifurone emulsifiable concentrate formulation, eliminating the need for separate preparation of a stock solution. The preparation method is simple, requires small dosage, is low-cost, and has a stable source. In vivo experiments have proven that within this concentration range, it has no adverse effects on the germination rate of tobacco seeds, root growth of tobacco seedlings, or aboveground growth. The quality of the tobacco seedlings is not affected, demonstrating good safety for the seedlings, and it does not require changes to the existing water and fertilizer management process for floating seedling cultivation. This invention utilizes a commercially available formulation that is highly efficient, safe, environmentally friendly, and has a stable and readily available source. It can be directly diluted and applied in tobacco seedling ponds, effectively inhibiting the growth of microalgae in the water and on the surface of the seedling trays. At the same time, it is safe for tobacco seed germination and seedling growth. It is of great significance for improving the quality of floating seedlings and ensuring the safety of tobacco production, and it is also a practical need for the green and sustainable development of the tobacco industry. Detailed Implementation
[0013] The following detailed description, in conjunction with preferred embodiments, details the specific implementation methods, features, and efficacy of the application and control methods of highly efficient flupyridine in tobacco seedling microalgae control based on the present invention.
[0014] Example 1:
[0015] A method for controlling microalgae in tobacco seedlings using highly effective flupyridine, comprising the following steps:
[0016] (1) Preparation of drug solution: Take high-efficiency flupyradifurone emulsifiable concentrate, dilute it with water according to the ratio, prepare a drug solution with an effective ingredient concentration of 100 mg / L, stir and mix well, and set aside for use;
[0017] (2) On the third day after sowing (early seedling stage), add the prepared solution in step (1) to the water in the seedling pond so that the final effective concentration of high-efficiency flupyridine in the seedling pond water is 10 mg / L. After stirring and mixing, carry out normal water and fertilizer management in the seedling pond.
[0018] Example 2:
[0019] A method for controlling microalgae in tobacco seedlings using highly effective flupyridine, comprising the following steps:
[0020] (1) Preparation of drug solution: Take high-efficiency flupyradifurone emulsifiable concentrate, dilute it with water according to the ratio, prepare a drug solution with an effective ingredient concentration of 300 mg / L, stir and mix well, and set aside for use;
[0021] (2) On the third day after sowing (early seedling stage), add the prepared solution in step (1) to the water in the seedling pond so that the final effective concentration of high-efficiency flupyridine in the seedling pond water is 30 mg / L. After stirring and mixing, carry out normal water and fertilizer management in the seedling pond.
[0022] Example 3:
[0023] A method for controlling microalgae in tobacco seedlings using highly effective flupyridine, comprising the following steps:
[0024] (1) Preparation of the drug solution: Take high-efficiency flupyradifurone emulsifiable concentrate, dilute it with water according to the ratio, prepare a drug solution with an effective ingredient concentration of 500 mg / L, stir and mix well, and set aside for use;
[0025] (2) On the third day after sowing (early seedling stage), add the prepared solution in step (1) to the water in the seedling pond so that the final effective concentration of high-efficiency flupyridine in the seedling pond water is 50 mg / L. After stirring and mixing, carry out normal water and fertilizer management in the seedling pond.
[0026] Experimental Example 1: Indoor toxicity determination of the dominant algae in the seedbed according to the present invention
[0027] 1.1 Experimental Materials and Methods
[0028] The high-efficiency flupyradifurone was prepared with sterile water to a concentration of 1.0 × 10⁻⁶. 4 The stock solution of high-efficiency flupyradifurone was diluted to a series of concentrations. Using a micropipette, the same volume of the diluted solution was added to the pure culture of three algae (Chlorella vulgaris, Chlamydomonas flatus, and Chlorella vulgaris) to prepare algal solutions with concentrations of 0.1 mg / L, 1 mg / L, 10 mg / L, 50 mg / L, and 100 mg / L. The solutions were then added to 24-well plates and incubated in a constant temperature and light incubator with a photoperiod of 12 h / 12 h, a light intensity of 1000 Lux, and a temperature of 25 ℃.
[0029] After 7 days of culture, the absorbance (OD) of each well at 680 nm was measured using a microplate reader. 680 The concentration value at which the inhibition rate is 50% was calculated using the inhibition rate probability value-concentration logarithmic method (EC). 50 ) and the concentration value with an inhibition rate of 95% (EC 95 Based on the probability value of the inhibition rate and the corresponding logarithm of the concentration, an independent regression equation was obtained using linear regression.
[0030] 1.2 Test Results
[0031] The results of the indoor toxicity test (see Table 1) show that the regression equations for the three dominant algal strains in the tobacco seedbed by the highly effective flupyradifurone of the present invention are y = 33.24 + 20.36x, y = 44.71 + 17.80x, and y = 34.15 + 20.01x, respectively. EC 50 The values were 6.76, 6.14, and 6.26 mg / L, respectively; EC 95 The values were 48.7, 42.5 and 47.1 mg / L, respectively.
[0032] Table 1. Inhibitory activity of highly effective flupyradifurone against dominant microalgae
[0033]
[0034] Experimental Example 2: Effect of this invention on microalgae control in tobacco seedling ponds and safety of tobacco seedlings.
[0035] 2.1 Experimental Materials and Methods
[0036] The tested tobacco variety was Yunyan 87. Conventional floating seedling raising was used, with seedling trays placed in the seedling pond and sown according to the normal procedure. The experiment included a control treatment (no pesticide application, and no high-efficiency fluroxypyr formulation added to the seedling pond water) and treatments from Examples 1-3, totaling four treatments.
[0037] Control treatment: Water and fertilizer management in the seedling pond was carried out according to normal procedures, without the application of high-efficiency flupyridine preparation solution.
[0038] Microalgae control efficacy survey: On the 30th day after application, the efficacy was assessed using a combination of visual inspection and absorbance (OD) measurement. 680 The absorbance of the seedling pond water was investigated using a assay method, and the control effect was calculated based on the difference in absorbance between the control and treatment waters. The formula for calculating the control effect is as follows:
[0039] Prevention and control effect (%) = [(control group OD)] 680 - Processing Group OD 680 ) / Control group OD 680 ]×100
[0040] Tobacco seedling safety survey: On the 30th day after pesticide application, the seedling emergence rate, plant height, stem circumference and fresh weight of 100 plants were observed and investigated by visual inspection.
[0041] 2.2 Test Results
[0042] The results of the microalgae control effect test (see Table 2) show that the high-efficiency flupyridine formulation of the present invention has a good control effect on microalgae in the seedling pond in the concentration range of 10-50 mg / L. Moreover, the control effect increases with the increase of the treatment concentration. The control effect reaches more than 85% on the 30th day after application, which is significantly better than the control treatment.
[0043] Table 2. Control effect of the chemical solutions in Examples 1-3 on microalgae in tobacco seedling ponds.
[0044]
[0045] The safety test results (see Table 3) show that, compared with the control, there were no significant differences in the growth indicators of tobacco seedlings after treatment in Examples 1-3, such as seedling emergence rate, plant height, stem circumference, root length, and fresh weight per 100 plants. The tobacco seedlings had normal leaf color and good root development, indicating that the high-efficiency fluopyram preparation within the concentration range described in this invention is safe for tobacco seed germination and seedling growth and does not affect the quality of tobacco seedlings.
[0046] Table 3. Safety effects of pesticide solutions in Examples 1-3 on tobacco seedling growth.
[0047]
[0048] In summary, this invention utilizes highly efficient flupyradifurone emulsifiable concentrate, which can effectively inhibit three dominant microalgae species in tobacco seedbeds. (EC) 95 The values are all less than 50 mg / L; and the agent, after being diluted with water, is directly applied to the tobacco seedling pond water at an effective concentration of 10-50 mg / L, which can significantly inhibit the growth and reproduction of microalgae in the seedling pond water and on the surface of the seedling trays. It is also safe for tobacco seed germination and seedling growth. It can be promoted and applied as a simple, effective and safe chemical control measure for the control of microalgae during the floating seedling stage of tobacco.
[0049] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications, equivalent changes, and alterations made to the above embodiments without departing from the technical essence of the present invention shall still fall within the scope of the present invention.
Claims
1. Application of a highly efficient flupyridine in the control of microalgae in tobacco seedlings.
2. The application of the highly efficient flupyridine haloxyfop-R-methyl as described in claim 1 in the control of microalgae in tobacco seedlings, characterized in that: Add a solution of highly effective flupyridine preparation to the tobacco seedling pond to make the effective concentration of highly effective flupyridine in the pond water 10-50 mg / L.
3. A method for controlling microalgae in tobacco seedling cultivation using highly efficient flupyridine, characterized in that: During the initial sowing stage of tobacco floating seedling cultivation, before the large cross stage, add high-efficiency flupyridine solution to the seedling pond water to achieve a concentration of 10-50 mg / L. After application, carry out normal water and fertilizer management in the seedling pond.
4. The method for controlling microalgae in tobacco seedling cultivation using highly efficient flupyridine as described in claim 3, characterized in that: The high-efficiency flupyradifurone solution is prepared by taking high-efficiency flupyradifurone emulsifiable concentrate, mixing it with water to form a solution with a concentration of 100-500 mg / L, and stirring until well mixed.