A pesticide composition containing fluoroalkenyl sulfoxide and its use

Abstract

The application belongs to the technical field of pesticide nematode killing, and discloses a pesticide composition containing fluazaindene and application thereof; the effective component of the pesticide composition comprises active ingredient A and active ingredient B; the active ingredient A is fluazaindene; the active ingredient B is any one of the following compounds: (formula 1), (formula 2), (formula 3), (formula 4) and (formula 5); and the mass ratio of the active ingredient A to the active ingredient B is 1:60-63:1. The pesticide composition has excellent control effect on plant nematodes.

Description

Technical Field

[0001] This invention belongs to the field of pesticide insecticide technology and discloses a pesticide composition containing fluorinated sulfone and its application. Background Technology

[0002] Nematodes are serious plant parasites that cause significant damage to agriculture. They can infest the roots of various food crops, cash crops, and fruits and vegetables, leading to slow growth, reduced yields, and deterioration in quality, resulting in huge economic losses to global agriculture every year. Currently, nematode control has become a crucial aspect of ensuring safe agricultural production, and the development of highly effective, low-toxicity, and long-lasting nematicides is one of the current research hotspots in the agricultural field.

[0003] Among existing nematicide technologies, chemical agents remain the mainstream control method. However, long-term use of single chemical nematicides easily leads to nematode resistance, resulting in a gradual decline in control efficacy. Furthermore, some agents pose problems such as environmental residues and high toxicity, failing to meet the needs of green agriculture development. While biological control is environmentally friendly, it suffers from limitations such as slow action and unstable control effects, making it difficult to meet the immediate control needs of large-scale agricultural production. Therefore, developing novel compound nematicide compositions that leverage the synergistic effects of compounds with different mechanisms of action to enhance control efficacy, delay resistance development, and reduce the dosage of single agents represents an effective approach to solving the current nematode control challenges. Summary of the Invention

[0004] Based on the above, the present invention provides a pesticide composition containing fluorinated sulfone, which has a significant synergistic effect on a variety of plant nematodes, a remarkable control effect, reduces the amount of pesticide used while slowing down the development of nematode resistance, and reduces pesticide residues.

[0005] The present invention also discloses the application of the above pesticide composition for the control of nematodes.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition containing fluorinated sulfone, wherein the active ingredient of the pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is fluorinated sulfone, and active ingredient B is any one of the following compounds: (Formula 1) (Formula 2) (Formula 3) (Formula 4) (Equation 5); the mass ratio of active ingredient A to active ingredient B is 1:60 to 63:1, or any value within the above range.

[0007] Furthermore, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:70 to 45:1, or any value within the above range. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 56:1, or any value within the above range. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:60 to 60:1, or any value within the above range. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:60 to 60:1, or any value within the above range. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 63:1, or any value within the above range.

[0008] Further, the active ingredient B is a compound of formula 1, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 45:1, or any value within the above range. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 38:1, or any value within the above range. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 45:1, or any value within the above range. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 45:1, or any value within the above range. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 36:1, or any value within the above range. Furthermore, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 25:1, or any value within the above range. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 24:1, or any value within the above range. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:1, or any value within the above range. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:1, or any value within the above range. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 18:1, or any value within the above range.

[0009] Further, the active ingredient B is a compound of formula 1, and the mass ratio of active ingredient A to active ingredient B is 1:70, 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:48, 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, 38:1, or 56:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:60, 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, 45:1, or 60:1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:60, 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, 45:1, or 60:1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:48, 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, 36:1, 54:1, or 63:1.

[0010] Further, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, or 38:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, or 45:1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, or 45:1. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, or 36:1. Furthermore, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, or 25:1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:18, 1:9, 1:3, 6:1, 12:1, or 24:1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, or 35:1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, or 35:1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:16, 1:8, 1:2, 9:1, or 18:1.

[0011] Furthermore, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 90% of the composition; Furthermore, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the composition.

[0012] Furthermore, in addition to the active ingredient, the composition also includes agriculturally permissible auxiliary ingredients selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, and carriers.

[0013] Furthermore, the composition is prepared into a pesticide-permitted formulation, wherein the formulation is a solid or liquid formulation.

[0014] Furthermore, the solid formulation is a water-dispersible granule, wettable powder, or pellet; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, seed treatment suspension, microemulsion, or dispersible oil suspension.

[0015] The present invention also discloses the application of the pesticide composition described above in the control of nematodes.

[0016] Furthermore, the nematode is any one or more of the following: root-knot nematodes, sporangioides nematodes, stem nematodes, scabra nematodes, and umbrella scabra nematodes. Furthermore, the nematodes mentioned are soybean cyst nematode and southern root-knot nematode.

[0017] The beneficial effects of this invention are as follows: (1) The pesticide composition of the present invention has a prominent synergistic effect. The two compounds complement each other through different mechanisms of action, and their nematode-killing effect is much higher than that of a single compound used alone. It can quickly kill nematodes of different ages. (2) The pesticide composition of the present invention can effectively delay the development of nematode resistance, reduce the selection pressure of single agent, and extend the service life of the agent; (3) The pesticide composition of the present invention reduces the amount of active ingredient used in a single agent, thereby reducing the risk of pesticide residue to the environment while ensuring the control effect and making it safer for crops; (4) The pesticide composition of the present invention has a long-lasting effect, reduces the number of times it is applied, lowers the cost of agricultural production, and is applicable to a wide range of crops. It has excellent control effects on a variety of crop nematode diseases. Detailed Implementation

[0018] To better illustrate the effective control effect of the present invention, and to make the technical solution, purpose, and advantages of the present invention clearer, the present invention is illustrated with the following specific embodiments. However, the present invention is not limited to these examples. The efficacy experiments of the present invention employ a combination of indoor bioassays and field trials.

[0019] Preparation Example: Preparation Process: 1. Seed treatment suspension processing technology: The active ingredient, adjuvant, and water are mixed and stirred evenly under high shear according to the formula, and then ground in a sand mill for 2.5 hours to make the average particle size reach 1-5 micrometers, thus obtaining the seed coating suspension.

[0020] Preparation Example 1: Seed Treatment Suspension of 2.5% Fluorine Sulfone • Formula 1 Compound (3:2) Formula composition: 1.5% fluoroalkyl sulfone, 1% Formula 1 compound, 1% Gelbert alcohol polyoxyethylene ether, 2.5% fatty alcohol polyoxyethylene ether sulfate, 2% ethylene glycol oxyethylene polyoxypropylene ether, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 4% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% potassium benzoate, deionized water to make up the balance.

[0021] Preparation Example 2: Seed Treatment Suspension of 2.8% Fluorine Sulfone • Formula 3 Compound (1:3) Formula composition: 0.7% fluoroalkyl sulfone, 2.1% Formula 3 compound, 1% alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% sodium octylphenol polyoxyethylene ether sulfonate, 2% sodium polycarboxylate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0022] Preparation Example 3: 3% Fluorine Sulfone • Formula 4 Compound Seed Treatment Suspension (2:1) Formula composition: 2% fluorosulfone, 1% Formula 4 compound, 1% castor oil polyoxyethylene ether, 3% sodium dioctyl succinate sulfonate, 2% fatty alcohol polyoxyethylene ether, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 4% rose red pigment, 4% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, deionized water to make up the balance.

[0023] Preparation Example 4: Seed Treatment Suspension of 2.4% Fluorine Sulfone • Formula 2 Compound (1:1) Formula composition: 1.2% fluorosulfone, 1.2% Formula 2 compound, 2.5% tristyrene-phenylphenol polyoxyethylene ether, 3% sodium methylnaphthalenesulfonate formaldehyde condensate, 2% glycerol fatty acid ester polyoxyethylene ether, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.5% sodium sorbate, deionized water to make up the balance.

[0024] Preparation Example 5: Seed Treatment Suspension of 2.7% Fluorine Sulfone • Formula 5 Compound (1:2) Formula composition: 0.9% fluoroalkyl sulfone, 1.8% Formula 3 compound, 2% phenylethyl phenol polyoxyethylene polyoxypropylene ether, 2% sodium polynaphthalene sulfonate, 2% sodium lignosulfonate, 1% polyacrylic acid emulsion, 0.3% xanthan gum, 6% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.8% sodium sorbate, deionized water to make up the balance.

[0025] Example 1: Combined toxicity test on soybean cyst nematode.

[0026] Experimental Basis: The experiment was conducted in accordance with NY / T 1833.1-2009 "Standard for Indoor Bioassay Tests of Pesticides - Nematicides Part 1: Inhibition of Plant Pathogenic Nematodes - Immersion Method".

[0027] Test subjects: Soybean cyst nematodes (Heterodera glycines) were mass-produced in diseased soil indoors, and cysts were collected by flotation separation method, sterilized and stored in a 4℃ refrigerator for later use.

[0028] Test reagents: Fluorine sulfone, and the technical grade of compounds of formula 1-5.

[0029] Reagent preparation: Dissolve each of the above reagents in a suitable solvent first, and then dilute with a 0.1% Tween-80 aqueous solution. Set up 5 series concentration gradients for each reagent, with an aqueous solution containing no reagent and the same solvent content as a control.

[0030] Preparation of nematode suspension: Select plump, brown sporangia of similar size and place them in a 200-mesh sieve. Place the sieve in a 35mm petri dish and sterilize with 1% NaClO solution for 3 minutes. Rinse 3-5 times with sterile water. Collect sporangia using a 200-mesh and 500-mesh sieve, grind them thoroughly with a grinding plug, rinse several times with sterile water, collect the egg suspension, and store at 4℃ for later use. Place the sterilized sporangia in sterile petri dishes for incubation, using sterile water as the incubation medium, ensuring the water slightly covers the sporangia. Incubate in a 25℃ incubator under dark conditions. After 4 days, begin collecting second-instar larvae. Adjust the concentration of the second-instar larval suspension to at least 100 nematodes per 1mL of suspension.

[0031] Experimental Method: Using a pipette, 3 mL of the drug solution was added sequentially from low to high concentration to each test tube. Then, 3 mL of the prepared nematode suspension was added to each test tube, ensuring equal volumes of drug and nematode suspension were thoroughly mixed. A specific volume of the mixture was then pipetted into the wells of a multi-well biochemical test plate, which was then capped. Each treatment was repeated four times, with an aqueous solution containing the same solvent and no drug as a control. The plates were incubated at 25°C for 24 hours, and the activity of the nematodes was examined. The relative lethality of each drug treatment was calculated.

[0032] Experimental investigation: Take 1 mL of the mixture from each treatment and observe the mortality of nematodes under a dissecting microscope. The number of nematodes observed in each replicate should not be less than 100. Record the total number of nematodes observed and the number of dead nematodes. Dead nematodes are often stiff and cannot bend or move when touched with a hair-thread needle or bamboo needle.

[0033] Calculation method: Based on the survey data, calculate the mortality rate for each treatment using the following formula, and keep the results to two decimal places.

[0034]

[0035] If the control mortality rate is less than 5%, no correction is needed; if the control mortality rate is between 5% and 15%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is greater than 15%, the trial needs to be repeated.

[0036] Statistical analysis: Based on the logarithmic values ​​of each drug concentration and the corresponding mortality rate, a statistical analysis system was used to analyze the data and determine the toxicity regression line and LC. 50 Values ​​and correlation coefficients are used to evaluate the activity of the test reagent on the biological sample.

[0037] The co-toxicity coefficient (CTC) of the mixture was calculated using Sun Yunpei's method to evaluate the type of combined effects.

[0038] A CTC ≥ 120 indicates a synergistic effect, a CTC ≤ 80 indicates an antagonistic effect, and a CTC between 80 and 120 indicates an additive effect.

[0039] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0040] In the formula: ATI —Measured toxicity index of the mixture; S LC of standard insecticides 50 The unit is milligrams per liter (mg / L); M LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0041]

[0042] In the formula: TTI —Theoretical toxicity index of the mixture; TI A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0043]

[0044] In the formula: CTC —Cotoxicity coefficient; ATI —Measured toxicity index of the mixture; TTI —Theoretical toxicity index of mixed preparations.

[0045] The experimental results are shown in the table below: Table 1. Results of indoor bioactivity tests of fluoxetine sulfone and compound of formula 1 on soybean cyst nematode. Fluorine sulfone (A) / / y = 4.1363 + 1.5479x 0.9964 3.6138 / Compound (B) of Formula 1 / / y = 4.3692 + 1.8280x 0.9906 2.2135 / A:B 1 70 y = 4.6165 + 1.5115x 0.9906 1.7935 124.095 A:B 1 35 y = 4.7143 + 1.5784x 0.9872 1.5171 147.491 A:B 1 25 y = 4.8022 + 1.4334x 0.9866 1.3739 163.548 A:B 1 15 y = 4.8002 + 1.7666x 0.9940 1.2975 174.831 A:B 1 5 y = 4.8853 + 1.4187x 0.9957 1.2047 196.424 A:B 3 2 y = 4.7431 + 1.7908x 0.9946 1.3915 207.259 A:B 5 1 y = 4.5999 + 1.5650x 0.9987 1.8017 181.446 A:B 15 1 y = 4.4985 + 1.5811x 0.9935 2.0759 167.462 A:B 25 1 y = 4.3685 + 1.7838x 0.9907 2.2595 156.139 A:B 45 1 y = 4.3539 + 1.4718x 0.9845 2.7478 129.732 A:B 65 1 y = 4.1585 + 1.5546x 0.9980 3.4778 102.924 Indoor bioactivity tests showed that the rational combination of fluoxetine sulfone and compound 1 had a significant synergistic effect on soybean cyst nematode. When the mass ratio of the two compounds was 1:70–45:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:35–45:1, the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect; and when the mass ratio was 1:25–25:1, the co-toxicity coefficient was greater than 150, indicating a remarkable synergistic effect.

[0046] Among them, when the mass ratio of the two is 1:70, 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect; when the mass ratio of the two is 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect; when the mass ratio of the two is 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, or 25:1, the co-toxicity coefficient is greater than 150, indicating a significant synergistic effect.

[0047] Table 2. Results of in vitro bioactivity tests of fluoxetine sulfone and compound of formula 3 on soybean cyst nematodes. Fluorine sulfone (A) / / y = 4.1363 + 1.5479x 0.9964 3.6138 / Compound (B) of Formula 3 / / y = 4.4366 + 1.4809x 0.9991 2.4013 / A:B 1 60 y = 4.4369 + 1.5376x 0.9947 2.3237 103.911 A:B 1 48 y = 4.6454 + 1.2879x 0.9968 1.8852 128.255 A:B 1 36 y = 4.6826 + 1.2285x 0.9985 1.8130 133.661 A:B 1 18 y = 4.6505 + 1.5281x 0.9958 1.6933 144.361 A:B 1 9 y = 4.6981 + 1.5485x 0.9986 1.5666 158.602 A:B 1 3 y = 4.7356 + 1.5440x 0.9960 1.4833 176.712 A:B 6 1 y = 4.5555 + 1.4502x 0.9997 2.0253 166.428 A:B 12 1 y = 4.5052 + 1.3182x 0.9924 2.3732 146.582 A:B 24 1 y = 4.4705 + 1.3138x 0.9948 2.5294 140.043 A:B 38 1 y = 4.3717 + 1.5093x 0.9988 2.6078 136.805 A:B 56 1 y = 4.3711 + 1.3866x 0.9978 2.8414 126.067 Indoor test results showed that the rational combination of fluoxetine sulfone and compound of formula 3 had a significant synergistic effect on soybean cyst nematode. When the mass ratio of the two compounds was 1:48–56:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:36–38:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect; and when the mass ratio was 1:18–24:1, the co-toxicity coefficient was greater than 140, indicating a remarkable synergistic effect.

[0048] Among them, the mass ratios of the two components are 1:48, 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, 38:1, and 56:1, with a co-toxicity coefficient greater than 120, indicating a synergistic effect; the mass ratios of the two components are 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, and 38:1, with a co-toxicity coefficient greater than 130, indicating a significant synergistic effect; and the mass ratios of the two components are 1:18, 1:9, 1:3, 6:1, 12:1, and 24:1, with a co-toxicity coefficient greater than 140, indicating a significant synergistic effect.

[0049] Table 3. Results of in vitro bioactivity tests of fluoxetine sulfone and compound of formula 4 on soybean cyst nematodes. Fluorine sulfone (A) / / y = 4.1363 + 1.5479x 0.9964 3.6138 / Compound (B) of Formula 4 / / y = 4.3428 + 1.4560x 0.9968 2.8273 / A:B 1 55 y = 4.4817 + 1.5090x 0.9987 2.2055 128.693 A:B 1 48 y = 4.4981 + 1.5684x 0.9925 2.0893 135.927 A:B 1 32 y = 4.6115 + 1.3613x 0.9962 1.9294 147.511 A:B 1 16 y = 4.5904 + 1.5203x 0.9971 1.8596 154.010 A:B 1 8 y = 4.6356 + 1.5210x 0.9963 1.7368 166.822 A:B 2 1 y = 4.6785 + 1.3885x 0.9961 1.7043 194.047 A:B 6 1 y = 4.5888 + 1.4992x 0.9976 1.8805 184.827 A:B 12 1 y = 4.5566 + 1.4771x 0.9984 1.9960 177.259 A:B 32 1 y = 4.4509 + 1.5248x 0.9963 2.2914 156.393 A:B 45 1 y = 4.4236 + 1.2688x 0.9873 2.8462 126.206 A:B 60 1 y = 4.3024 + 1.4492x 0.9968 3.0295 118.745 Indoor test results showed that combining fluoxetine sulfone with compound 4 at an appropriate mass ratio had a synergistic effect on soybean cyst nematode. A mass ratio of 1:55–45:1 resulted in a co-toxicity coefficient greater than 120, indicating a synergistic effect; a mass ratio of 1:48–32:1 resulted in a co-toxicity coefficient greater than 130, indicating a significant synergistic effect; and a mass ratio of 1:32–32:1 resulted in a co-toxicity coefficient greater than 140, indicating a remarkable synergistic effect.

[0050] Among them, when the mass ratio of the two is 1:55, 1:48, 1:32, 1:16, 1:8, 2:1, 6:1, 12:1, 32:1, or 45:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect; when the mass ratio of the two is 1:48, 1:32, 1:16, 1:8, 2:1, 6:1, 12:1, or 32:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect; when the mass ratio of the two is 1:32, 1:16, 1:8, 2:1, 6:1, 12:1, or 32:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0051] Table 4. Results of in vitro bioactivity tests of fluoxetine sulfone and compound of formula 2 on soybean cyst nematodes. Fluorine sulfone (A) / / y = 4.1363 + 1.5479x 0.9964 3.6138 / Compound (B) of Formula 2 / / y = 4.2267 + 1.5634x 0.9930 3.1233 / A:B 1 60 y = 4.3522 + 1.5973x 0.9958 2.5444 123.026 A:B 1 45 y = 4.5078 + 1.3564x 0.9925 2.3060 135.843 A:B 1 35 y = 4.4318 + 1.6909x 0.9955 2.1679 144.616 A:B 1 15 y = 4.4946 + 1.5938x 0.9996 2.0755 151.772 A:B 1 5 y = 4.5914 + 1.4653x 0.9990 1.9004 168.154 A:B 1 1 y = 4.6807 + 1.2610x 0.9936 1.7914 187.043 A:B 5 1 y = 4.5587 + 1.4318x 0.9958 2.0336 173.172 A:B 15 1 y = 4.4721 + 1.5681x 0.9901 2.1711 164.832 A:B 35 1 y = 4.3989 + 1.6348x 0.9969 2.3320 154.293 A:B 45 1 y = 4.3215 + 1.6078x 0.9915 2.6424 136.297 A:B 60 1 y = 4.2489 + 1.6420x 0.9975 2.8670 125.724 Indoor test results showed that combining sulfone and compound 2 in an appropriate mass ratio had a significant synergistic effect on soybean cyst nematode; when the mass ratio was 1:60~60:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:45~45:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect; and when the mass ratio was 1:35~35:1, the co-toxicity coefficient was greater than 140, indicating a remarkable synergistic effect.

[0052] Among them, when the mass ratio of the two components is 1:60, 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, 45:1, or 60:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect; when the mass ratio of the two components is 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, or 45:1, the co-toxicity coefficient is greater than 130, indicating a synergistic effect; when the mass ratio of the two components is 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, or 35:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0053] Table 5. Results of in vitro bioactivity tests of fluoxetine sulfone and compound of formula 5 on soybean cyst nematodes. Fluorine sulfone (A) / / y = 4.1363 + 1.5479x 0.9964 3.6138 / Compound (B) of Formula 5 / / y = 4.1969 + 1.5390x 0.9984 3.3254 / A:B 1 56 y = 4.3323 + 1.3568x 0.9986 3.1054 107.235 A:B 1 48 y = 4.3634 + 1.5342x 0.9955 2.5998 128.119 A:B 1 32 y = 4.4022 + 1.5797x 0.9955 2.3901 139.470 A:B 1 16 y = 4.3259 + 1.8506x 0.9939 2.3134 144.423 A:B 1 8 y = 4.4411 + 1.6644x 0.9985 2.1666 154.858 A:B 1 2 y = 4.5911 + 1.4904x 0.9921 1.8807 181.649 A:B 9 1 y = 4.5405 + 1.4460x 0.9984 2.0786 172.363 A:B 18 1 y = 4.4987 + 1.2857x 0.9975 2.4543 146.575 A:B 36 1 y = 4.3481 + 1.5539x 0.9987 2.6273 137.226 A:B 54 1 y = 4.3073 + 1.5570x 0.9986 2.7855 129.532 A:B 63 1 y = 4.3259 + 1.4603x 0.9983 2.8949 124.664 Indoor test results showed that the rational combination of fluoxetine sulfone and compound 5 had a significant synergistic effect on soybean cyst nematode. When the mass ratio of the two compounds was 1:48–63:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect; when the mass ratio was 1:32–36:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect; and when the mass ratio was 1:16–18:1, the co-toxicity coefficient was greater than 140, indicating a remarkable synergistic effect.

[0054] Among them, the mass ratios of the two components are 1:48, 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, 36:1, 54:1, and 63:1, with a co-toxicity coefficient greater than 120, indicating a synergistic effect; the mass ratios of the two components are 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, and 36:1, with a co-toxicity coefficient greater than 130, indicating a significant synergistic effect; and the mass ratios of the two components are 1:16, 1:8, 1:2, 9:1, and 18:1, indicating a significant synergistic effect.

[0055] Example 2: Field efficacy trial for controlling soybean cyst nematode.

[0056] Experimental crop: soybean (Zhonghuang 30).

[0057] Overview of the experimental site: The experimental site is located in Chenhu Village, Dongzhi Town, Xifeng District, Qingyang City, Gansu Province. The soil in the experimental site is sandy loam with medium to high fertility. Soybeans have been planted there for many consecutive years, and soybean cyst nematode disease has occurred quite severely.

[0058] Experimental method: The experiment consisted of 8 treatments, with 4 replicates for each treatment. Each experimental plot was 10 m² in size. 2 All experimental plots were arranged in a randomized block design. Soybeans were treated with a seed treatment suspension before sowing, then air-dried. The experimental agents and dosages are shown in Table 6.

[0059] Experimental Investigation: The efficacy of the pesticide was investigated in each plot before application and after harvest. Before application, soil samples were collected at five points in each plot, from the 0-20cm depth. The samples were brought back to the laboratory, air-dried, mixed thoroughly, and 100g of soil was randomly weighed and placed in a plastic basin. Tap water was added and stirred thoroughly until well mixed. After standing for 2-3 minutes, the suspension was filtered through a 26-mesh and a 60-mesh sieve, repeated 3-4 times. Residue on the 26-mesh sieve was rinsed with clean water, and the 60-mesh sieve was rinsed slowly with a low stream of water. After rinsing, the residue was washed onto nylon gauze in a wash bottle, recorded, and repeated 4 times. The number of sporangia was counted under a dissecting microscope. After soybean harvest, the sporangia count in each plot was calculated using the same method. The sporangia reduction rate and control effect were calculated. The experimental results are shown in Table 6.

[0060] Formula for calculating drug efficacy:

[0061] The results of the field efficacy trials are shown below: Table 6 Field efficacy trials for controlling soybean cyst nematodes 1 2.4% Fluorine Sulfone • Formula 2 Compound Seed Treatment Suspension (1:1) 1000g / 100kg -4.34 92.28 2 3% Fluorine Sulfone • Formula 4 Compound Seed Treatment Suspension (2:1) 1000g / 100kg -2.26 95.99 3 2.8% Fluorine Sulfone • Formula 3 Compound Seed Treatment Suspension (1:3) 1000g / 100kg -6.50 88.45 4 2.7% Fluorine Sulfone • Formula 5 Compound Seed Treatment Suspension (1:2) 1000g / 100kg -5.07 90.99 5 2.5% Fluorine Sulfone • Formula 1 Compound Seed Treatment Suspension (3:2) 1000g / 100kg -1.47 97.38 6 2.9% Pyraclostrobin·Methoxyfenozide·Avermectin Seed Treatment Suspension Concentrate (1.8+0.5+0.6) 1110g / 100kg -13.32 76.34 7 3% Fluorine Sulfone Seed Treatment Suspension 2200g / 100kg -14.81 73.69 8 Blank control / -61.21 - Field efficacy trials showed that the pesticide composition of this invention has excellent control effects against soybean cyst nematodes. The control efficacy of each compound formulation was higher than 88%, significantly better than other single-agent controls.

[0062] In summary, through indoor toxicity testing and field efficacy trials, the pesticide composition of the present invention has a good control effect on nematodes, is safe for target crops, and has significant control efficacy. It is superior to single agents in delaying the development of resistance and prolonging the duration of action, and can effectively reduce production and usage costs.

Claims

1. A pesticide composition containing fluorinated sulfone, characterized in that, The active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein active ingredient A is fluoroalkyl sulfone, and active ingredient B is any one of the following compounds: (Formula 1) (Formula 2) (Formula 3) (Formula 4) (Equation 5); The mass ratio of active ingredient A to active ingredient B is 1:60~63:

1.

2. The pesticide composition according to claim 1, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:70~45:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:48~56:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:60~60:1; The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:60~60:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:48~63:

1.

3. The pesticide composition according to claim 2, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:35~45:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:36~38:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 45:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:45~45:1; The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:32~36:1; Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 25:1; The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:18~24:1; The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 35:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:35~35:1; The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:16 to 18:

1.

4. The pesticide composition according to claim 1, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:70, 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:48, 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, 38:1, or 56:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:60, 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, 45:1, or 60:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:60, 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, 45:1, or 60:

1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:48, 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, 36:1, 54:1, or 63:

1.

5. The pesticide composition according to claim 4, characterized in that, The active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, 25:1, or 45:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:36, 1:18, 1:9, 1:3, 6:1, 12:1, 24:1, or 38:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, or 45:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:45, 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, 35:1, or 45:

1. The active ingredient B is a compound of formula 5, and the mass ratio of the active ingredient A to the active ingredient B is 1:32, 1:16, 1:8, 1:2, 9:1, 18:1, or 36:

1. Preferably, the active ingredient B is a compound of formula 1, and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:5, 3:2, 5:1, 15:1, or 25:

1. The active ingredient B is a compound of formula 3, and the mass ratio of the active ingredient A to the active ingredient B is 1:18, 1:9, 1:3, 6:1, 12:1, or 24:

1. The active ingredient B is a compound of formula 4, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, or 35:

1. The active ingredient B is a compound of formula 2, and the mass ratio of the active ingredient A to the active ingredient B is 1:35, 1:15, 1:5, 1:1, 5:1, 15:1, or 35:

1. The active ingredient B is a compound of formula 5, and the mass ratio of active ingredient A to active ingredient B is 1:16, 1:8, 1:2, 9:1, or 18:

1.

6. The pesticide composition according to claim 1, characterized in that, The total weight of active ingredient A and active ingredient B accounts for 0.1% to 90% of the composition, preferably 0.1% to 80%.

7. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the composition also includes agriculturally permissible auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, thickeners, disintegrants, emulsifiers, defoamers, preservatives, stabilizers, synergists, and carriers.

8. The pesticide composition according to claim 1, characterized in that, The composition is prepared into a pesticide formulation, which may be a solid or liquid formulation.

9. The pesticide composition according to claim 8, characterized in that, The solid formulation is a water-dispersible granule, wettable powder, or pellet; the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, seed treatment suspension, microemulsion, or dispersible oil suspension.

10. The application of the pesticide composition according to any one of claims 1-9 in the control of nematodes, characterized in that, The nematodes mentioned are soybean cyst nematode and southern root-knot nematode.

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