A method for controlling cassava mosaic disease
Patent Information
- Application Number
- CN202610962474.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-30
- Publication Date
- 2026-09-25
AI Technical Summary
但在实际应用过程中，单一成分药剂防治效果有限，且长期使用以使靶标产生耐药性，导致防治效果降低甚至失效
本发明在木薯种植过程中，施用抗媒介昆虫药剂阻断烟粉虱传播木薯花叶病毒，施用抗花叶病毒药剂抑制木薯花叶病病情发展，两种药剂结合可有效实现木薯花叶病的防治及病毒传播；且由硫环虫酰胺与环氧虫啶复配而成的抗媒介昆虫药剂及由香菇多糖与谷甾醇复配而成的抗花叶病毒药剂分别对靶标烟粉虱、病毒表现出联合增效作用，可提高防治效果，降低抗药性风险，进而可为木薯花叶病的防治提供支持。
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of cassava virus disease control technology, specifically relating to a method for controlling cassava mosaic disease. Background Technology
[0002] Cassava mosaic virus disease is one of the most serious diseases affecting cassava cultivation worldwide. In Asia, it is caused by two strains: Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV).
[0003] Cassava mosaic virus can occur throughout the entire growth and development stage of cassava. Young cassava plants are more susceptible to the disease, with typical symptoms including mosaic patterns across the entire plant. Subsequently, during tuber formation, infected cassava plants first show symptoms on young leaves, with small, discolored, yellowing spots expanding and forming a distinct mosaic pattern on the surrounding healthy green leaf tissue. Infected plants are typically stunted, with reduced tuber production and smaller tubers; in severe cases, underground tubers may not even form, leading to reduced yield or crop failure.
[0004] Furthermore, under field conditions, the disease is primarily transmitted via whiteflies. Whiteflies acquire the virus by feeding on infected cassava leaves, with a minimum incubation period of 8 hours. They can infect healthy cassava plants within 10 minutes by feeding on them and maintain the virus's ability to infect healthy cassava plants for up to 9 days. Therefore, in actual production, controlling whiteflies can effectively block the spread of cassava mosaic virus.
[0005] During cassava cultivation and growth, chemical agents can be applied to control cassava mosaic virus and its vectors, thus achieving the prevention and control of cassava mosaic disease. However, in practical applications, the control effect of single-component agents is limited, and long-term use can lead to drug resistance, resulting in reduced or even ineffective control. Researching and screening synergistic compound combinations can improve the control effect on the target virus while reducing the risk of drug resistance. Therefore, researching synergistic compound combinations is of great significance for the prevention and control of cassava mosaic disease.
[0006] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention
[0007] The purpose of this invention is to provide a method for preventing and controlling cassava mosaic disease, so as to solve the problems existing in the prior art.
[0008] To achieve the above objectives, the present invention provides the following technical solution: A method for controlling cassava mosaic disease includes: during cassava planting, applying an insecticide to block the transmission of cassava mosaic virus by whiteflies and / or applying an anti-mosaic virus agent to inhibit the development of cassava mosaic disease; wherein the active ingredient of the insecticide is a compound of thiocyclam and cyclooxygenated acetamiprid; and the active ingredient of the anti-mosaic virus agent is a compound of lentinan and sitosterol.
[0009] Furthermore, the mass ratio of the compound of thiocarbamate and epoxim is 1:7-1.
[0010] Furthermore, the characteristic feature is that the mass ratio of the lentinan to sitosterol is 1-5:5-1.
[0011] The present invention also provides the application of the aforementioned insecticide in the control of cassava whitefly or cassava mosaic virus disease.
[0012] This invention also provides the application of the aforementioned anti-mosaic virus agent in the control of cassava mosaic virus disease. The present invention also provides a method for preventing and controlling the cassava mosaic disease or the application described in any one of claims 4-5, characterized in that the cassava mosaic disease is caused by infection with Sri Lanka cassava mosaic virus (SLCMV).
[0013] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes an insecticide against vector pests to block the transmission of cassava mosaic virus by whiteflies during cassava cultivation, and an anti-mosaic virus agent to inhibit the development of cassava mosaic disease. The combination of these two agents effectively controls cassava mosaic disease and the spread of the virus. Furthermore, the insecticide against vector pests, composed of thiophanate-methyl and cyclooxygenated acetamiprid, and the anti-mosaic virus agent, composed of lentinan and sitosterol, exhibit synergistic effects against the target whiteflies and the virus, respectively. This enhances the control effect, reduces the risk of drug resistance, and thus provides support for the prevention and control of cassava mosaic disease. Detailed Implementation
[0014] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.
[0015] Example: Indoor bioactivity test 1. Indoor activity test of cassava whitefly 1.1 Tested Pests Whiteflies were collected from cassava plants and tested for multiple generations in the laboratory using fresh, clean cassava leaves that had not been exposed to pesticides. Healthy adult whiteflies of uniform size were selected as the test subjects.
[0016] 1.2 Test reagents 97% thiophanate-methyl technical grade, 96% cyclooxygenated technical grade After the original drug is dissolved, it is diluted with 0.1% Triton X-100 aqueous solution to form a single-dose stock solution; multiple formulations are set up, and each single agent and each formulation mixture is further diluted with 0.1% Triton X-100 aqueous solution to form 5 mass concentration gradients. All drugs are prepared and used immediately.
[0017] 1.3 Test Methods The leaf immersion method was used. Fresh, clean cassava leaves that had not been exposed to pesticides were used to create leaf discs using a 22mm punch. The leaf discs were immersed in the pesticide solution for 10 seconds, then removed and air-dried. They were then placed in a glass bottle with moisturizing agar at the bottom, and the leaf discs were gently pressed to ensure close contact between the leaves and the agar. Twenty adult whiteflies were introduced into the glass bottle, and the bottle opening was then sealed with a breathable cotton plug to allow for ventilation and prevent adult escape. The bottles were then placed in a light incubator for observation. Each treatment was performed in triplicate, with leaf discs treated with 0.1% Triton X-100 serving as a blank control. After 24 hours, the insects were observed; those that did not move when touched with a pen tip were considered dead. The corrected mortality rate for each treatment was calculated.
[0018]
[0019] In the above formula: P -- Mortality rate, in percentages. K --The number of dead insects; N --Total number of insects treated.
[0020]
[0021] In the above formula: P 1 -- Corrected mortality rate, in % P t --Case mortality rate, in % P 0 -- Mortality rate in the blank control group, in percent.
[0022] 1.4 Data Analysis Regression analysis was performed on the logarithmic values of the drug concentrations for each treatment and the corresponding corrected mortality probability values to calculate the LC-1 of each treatment. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method, and the synergistic effect of the agent was evaluated based on the calculated CTC value: CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect. The results are shown in Table 1.
[0023] Table 1. Results of indoor activity tests of the combination of thiocyclam and epoxim against cassava whiteflies.
[0024] Table 1 shows that within a mass ratio of 1:7-1, the co-toxicity coefficients of thiocyclam and epoxiconazole against cassava whiteflies ranged from 120.2277 to 415.9056, all greater than 120, demonstrating a synergistic effect and improving the control efficacy against cassava whiteflies. This indicates that the combination of thiocyclam and epoxiconazole in this invention helps to block the transmission of cassava mosaic virus.
[0025] 2. Indoor activity test of cassava mosaic virus 2.1 Test Toxin Fresh leaves of Nanzhi 199 cassava plants infected with cassava mosaic virus were collected from the cassava base. The virus was identified as Sri Lanka cassava mosaic virus (SLCMV). Diseased leaf samples were mixed with PBS buffer (0.01 mol / L, pH 7.2) and 500-mesh quartz sand, ground into a homogenate, and then filtered through four layers of gauze to obtain the virus solution. The ratio of diseased leaf mass to PBS buffer volume was 50 mg: 1 L.
[0026] 2.2 Test reagents 96% Lentinan technical grade, 98% S-sitosterol (CAS: 83-46-5) technical grade After the original drug is dissolved, it is diluted with 0.1% Triton X-100 aqueous solution to form a single-dose stock solution; multiple formulations are set up, and each single agent and each formulation mixture is further diluted with 0.1% Triton X-100 aqueous solution to form 5 mass concentration gradients. All drugs are prepared and used immediately.
[0027] 2.3 Test Methods Healthy cassava seedlings of the "Nanzhi 199" variety with uniform growth and leaf number were selected. A layer of carborundum was sprayed onto the surface of each leaf, and virus solution was gently rubbed onto the leaves with a brush for inoculation. After inoculation, the leaf surfaces were gently rinsed with water, dried, and then sprayed with the prepared pesticide solution every 7 days for a total of 3 applications. Each pesticide solution was used to treat 20 seedlings, with 3 replicates. A treatment using a 0.1% Triton X-100 aqueous solution served as a blank control. Disease incidence was assessed before spraying and 3 months after the last spraying, and the disease index and relative control efficacy were calculated. The grading criteria referenced the "Standard Operating Procedure (SOP) for Identifying Cassava Resistant to Sri Lankan Cassava Mosaic Virus (SLCMV)".
[0028]
[0029]
[0030] 2.4 Disease severity table Table 2 Disease Severity Table
[0031] 2.5 Data Analysis Regression analysis was performed on the logarithmic values of pesticide concentrations for each treatment and the relative efficacy probability values for each treatment to calculate the EC50 of each treatment. 50 The CTC value of the mixture was calculated using the Sun Yunpei method, and the action type of the drug was evaluated based on the CTC value. The results are shown in Table 3.
[0032] Table 3. Results of indoor activity tests of lentinan combined with sitosterol against Sri Lanka cassava mosaic virus (SLCMV).
[0033] Table 3 shows that within a mass ratio of 1-5:5-1, the co-viral coefficients of lentinan and sitosterol against Sri Lanka cassava mosaic virus (SLCMV) ranged from 124.5705 to 141.6154, all greater than 120, demonstrating a synergistic effect and improving the control efficacy against cassava mosaic virus. This indicates that the combination of lentinan and sitosterol in this invention helps inhibit the development of cassava mosaic virus.
[0034] In summary, this invention utilizes insecticides to block the transmission of cassava mosaic virus by whiteflies during cassava cultivation, and anti-mosaic virus agents to inhibit the development of cassava mosaic disease. The combination of these two agents effectively controls cassava mosaic disease and the spread of the virus. Furthermore, the insecticides composed of thiophanate-methyl and cyclooxygenated acetamiprid, and the anti-mosaic virus agents composed of lentinan and sitosterol, exhibit synergistic effects against the target whiteflies and the virus, respectively, which can improve control efficacy, reduce the risk of drug resistance, and thus provide support for the control of cassava mosaic disease.
Claims
1. A method for controlling cassava mosaic disease, characterized in that, include: During cassava cultivation, the application of insecticides against vector-borne pests can block the transmission of cassava mosaic virus by whiteflies, and / or the application of anti-mosaic virus agents can inhibit the development of cassava mosaic disease. The active ingredient of the insecticide is a compound of thiocyclamide and epoxiconazole; The active ingredient of the anti-mosa virus agent is a compound of lentinan and sitosterol.
2. The method for controlling cassava mosaic disease according to claim 1, characterized in that, The mass ratio of the compound of thiocyclam and epoxim is 1:7-1.
3. The method for controlling cassava mosaic disease according to claim 1, characterized in that, The mass ratio of the lentinan to sitosterol is 1-5:5-1.
4. The application of the insecticide according to claim 1 in the control of cassava whitefly or cassava mosaic virus disease.
5. The application of the anti-mosaic virus agent according to claim 1 in the prevention and control of cassava mosaic virus disease.
6. The method for controlling cassava mosaic disease according to any one of claims 1-3 or the application according to any one of claims 4-5, characterized in that, The cassava mosaic disease is caused by Sri Lankan cassava mosaic virus (SLCMV).