Use of resveratrol in the preparation of a product for improving oxidative stress in poultry

Abstract

The present application belongs to the technical field of poultry feed. More specifically, the present application provides the use of resveratrol in the preparation of a product for improving the oxidative stress of poultry. The present application research finds that the growth performance of broilers in the oxidative stress state is inhibited, the serum biochemical indexes of broilers are disordered, the intestinal morphology is destroyed, and the meat quality is poor due to oxidative stress, the pH value of meat increases, the brightness of meat decreases, and the water holding capacity of meat decreases. By adding a small amount of resveratrol in the feed, the oxidative stress state of broilers can be effectively improved, the negative effects brought by oxidative stress can be alleviated, the growth performance, antioxidant state, serum biochemical indexes and intestinal structure of broilers are restored, and the meat quality is significantly improved. In addition, resveratrol is a natural substance and widely exists in plants, which meets the green environmental protection requirements. The feed added with resveratrol of the present application is suitable for the feeding of broilers in the oxidative stress state, and has good application prospect and value.

Description

Technical Field

[0001] This invention belongs to the field of poultry feed technology. More specifically, it relates to the application of resveratrol in the preparation of products that improve oxidative stress in poultry. Background Technology

[0002] With population growth and rising living standards, the demand for animal products has been increasing, driving the rapid development of intensive livestock farming in recent decades. The growth rate, feed conversion efficiency, and disease resistance of livestock and poultry have significantly improved. However, intensive production models make poultry more susceptible to various stressors during production and transportation, such as stocking density, thermal environment, and transport stress. Oxidative stress has had a profound impact on the poultry industry. Under oxidative stress, the excessive production of free radicals can lead to digestive system abnormalities and enterogenic infections, affecting nutrient absorption and utilization and reducing production performance. Furthermore, long-term oxidative stress can cause oxidative damage, weakened immune function, and decreased meat quality in animals.

[0003] Therefore, exploring effective strategies to alleviate oxidative stress, enhance antioxidant capacity, and maintain immune function and gut health is crucial for improving poultry production efficiency and ensuring meat quality. Summary of the Invention

[0004] The present invention aims to provide the application of resveratrol in the preparation of products that improve oxidative stress in poultry.

[0005] The above-mentioned objective of this invention is achieved through the following technical solution: Resveratrol (trans-3,4',5-trihydroxystilbene) is a natural non-flavonoid polyphenol widely found in plants. This invention has found that broiler chickens under oxidative stress exhibit inhibited growth performance, disordered serum biochemical indicators, and disrupted intestinal morphology. Furthermore, oxidative stress leads to poor meat quality, increased pH, decreased meat brightness, and reduced water-holding capacity. This invention, by adding trace amounts of resveratrol to feed, effectively improves the oxidative stress state of broiler chickens, alleviates the negative effects of oxidative stress, restores growth performance, antioxidant status, serum biochemical indicators, and intestinal structure, and significantly improves meat quality. Therefore, this invention claims protection for the following: This invention provides the application of resveratrol in the preparation of products that improve oxidative stress in poultry.

[0006] This invention provides the application of resveratrol in the preparation of feed that improves oxidative stress in poultry.

[0007] Specifically, the poultry referred to are broiler chickens.

[0008] More specifically, the poultry referred to are yellow-feathered broiler chickens.

[0009] Specifically, the improvement of oxidative stress in poultry refers to the improvement of growth inhibition caused by oxidative stress.

[0010] The present invention also provides a feed, which, by weight, contains the following components: 0.1-0.3 parts resveratrol, 70-77 parts energy feed, 19.5-29.2 parts protein feed, 0.1-0.2 parts premix, and 0.15-0.3 parts additives.

[0011] As an alternative implementation, the energy feed includes corn and lard.

[0012] As an alternative implementation, the protein feed is one or more of soybean meal, corn gluten meal, lysine, methionine, and threonine.

[0013] As an alternative implementation, the mineral feed is one or more of limestone powder, dicalcium phosphate, and sodium chloride.

[0014] As an alternative implementation, the additive is sodium bicarbonate and choline chloride.

[0015] As an alternative implementation, the premix contains vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, pantothenic acid, niacin, folic acid, biotin, iron, copper, manganese, zinc, iodine, and selenium.

[0016] As an alternative specific implementation, the feed, by weight, contains the following components: 0.1-0.3 parts resveratrol, 68-74 parts corn, 15-20 parts soybean meal, 4-8 parts corn gluten meal, 2-3 parts lard, 1-1.5 parts limestone, 0.5-1.5 parts dicalcium phosphate, 0.3-0.9 parts lysine, 0.2-0.3 parts salt, 0.15-0.2 parts methionine, 0.1-0.15 parts sodium bicarbonate, 0.05-0.15 parts choline chloride, 0.05-0.1 parts threonine, and 0.1-0.2 parts premix.

[0017] As an alternative specific implementation, the feed, by weight, contains the following components: 0.1-0.3 parts resveratrol, 70.8 parts corn, 16.9 parts soybean meal, 6 parts corn gluten meal, 2.5 parts lard, 1.31 parts limestone powder, 1 part dicalcium phosphate, 0.6 parts lysine, 0.27 parts salt, 0.18 parts DL-methionine, 0.12 parts sodium bicarbonate, 0.1 parts choline chloride, 0.07 parts threonine, and 0.15 parts premix.

[0018] Preferably, the feed, by weight, contains the following components: 0.3 parts resveratrol, 70.8 parts corn, 16.9 parts soybean meal, 6 parts corn gluten meal, 2.5 parts lard, 1.31 parts limestone powder, 1 part dicalcium phosphate, 0.6 parts lysine, 0.27 parts salt, 0.18 parts DL-methionine, 0.12 parts sodium bicarbonate, 0.1 parts choline chloride, 0.07 parts threonine, and 0.15 parts premix.

[0019] The application of the above-mentioned feed in the preparation of products that improve oxidative stress in poultry should also be within the scope of protection of this invention.

[0020] Ming has the following beneficial effects: This invention reveals that broiler chickens under oxidative stress exhibit inhibited growth performance, disordered serum biochemical indicators, and disrupted intestinal morphology. Furthermore, oxidative stress leads to deteriorated meat quality, increased meat pH, decreased meat brightness, and reduced water-holding capacity. This invention, by adding trace amounts of resveratrol to the feed, effectively improves the oxidative stress state of broilers, alleviates the negative effects of oxidative stress, restores broiler growth performance, antioxidant status, serum biochemical indicators, and intestinal structure, and significantly improves meat quality.

[0021] Furthermore, resveratrol is a natural non-flavonoid polyphenol widely found in plants. It is safe and environmentally friendly, without polluting the environment or leaving harmful residues, thus meeting green environmental protection requirements. The feed supplemented with resveratrol according to this invention is suitable for broiler chickens under oxidative stress, and has excellent application prospects and value. Attached Figure Description

[0022] Figure 1 The molecular structure and source of resveratrol.

[0023] Figure 2 A flowchart for the experimental design.

[0024] Figure 3 The results show the growth performance of yellow-feathered broilers under different treatments.

[0025] Figure 4 Results of calf muscle mass determination in yellow-feathered broiler chickens under different treatments.

[0026] Figure 5 The results show the determination of breast muscle mass in yellow-feathered broiler chickens under different treatments.

[0027] Figure 6 Serum biochemical parameters of yellow-feathered broilers under different treatments were measured. Figure 7 MDA levels in serum, liver, and muscle of yellow-feathered broilers under different treatments.

[0028] Figure 8 The serum, liver, and muscle SOD levels of yellow-feathered broilers under different treatments were measured.

[0029] Figure 9 Serum, liver, and muscle GSH-PX levels in yellow-feathered broilers under different treatments.

[0030] Figure 10 CAT levels in serum, liver, and muscle of yellow-feathered broilers under different treatments.

[0031] Figure 11 The levels of MDA and SOD in the duodenum, jejunum, and ileum of yellow-feathered broilers under different treatments.

[0032] Figure 12 Cross-sectional views of the duodenum, jejunum, and ileum of yellow-feathered broilers under different treatments (magnification 50 x; scale bar = 200 μm).

[0033] Figure 13 The results of measuring villus height, crypt depth and ratio in the duodenum, jejunum and ileum of yellow-feathered broilers under different treatments.

[0034] Figure 14 The expression levels of GPX2, ZO-1, SOD, CAT, PPAR-γ and Occludin genes in the duodenum of yellow-feathered broiler chickens under different treatments were determined.

[0035] Figure 15 The expression levels of GPX2, ZO-1, SOD, CAT, PPAR-γ and Occludin genes in the ileum of yellow-feathered broiler chickens under different treatments were determined. Detailed Implementation

[0036] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0037] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0038] The resveratrol used in this experiment (purity ≥99%) was provided by Guangzhou Jingjing Biotechnology Co., Ltd. It is a white to pale yellow powder, insoluble in water, readily soluble in ethanol, and odorless. The specific extraction process is as follows: using Polygonum cuspidatum rhizome as raw material, it is obtained through fermentation, extraction, concentration, dissolution, separation, filtration, further concentration, and separation.

[0039] Dexamethasone (DEX) (2 mg / mL) was provided by Guangzhou Baiyunshan Tianxin Pharmaceutical Co., Ltd.

[0040] In the following examples, data were analyzed using one-way ANOVA with GraphPad Prism 9.0 software, and Tukey's multiple comparison analysis was used to analyze differences between groups. P < 0.05 was considered statistically significant, and 0.05 ≤ P < 0.10 was considered to indicate a significant trend. Results are expressed as mean ± standard error.

[0041] Example 1 Experimental Design The molecular structure and sources of resveratrol, such as Figure 1 As shown, the flowchart of the experimental design is as follows: Figure 2 As shown.

[0042] I. Setting up the experimental control group and treatment group One hundred and twenty healthy 45-day-old yellow-feathered broiler chickens, half male and half female, were randomly divided into five treatment groups, with four replicates in each treatment group and six chickens per replicate. The treatment groups were as follows: Control group (CON group): fed a basal corn-soybean meal diet; Dexamethasone group (DEX group): fed a basal corn-soybean meal diet; Dexamethasone supplemented with 0.1% resveratrol group (DR1 group): fed a basic corn-soybean meal diet supplemented with 0.1% resveratrol; Dexamethasone supplemented with 0.2% resveratrol (DR2 group): fed a corn-soybean meal-based diet supplemented with 0.2% resveratrol. Dexamethasone supplemented with 0.3% resveratrol (DR3 group): fed a corn-soybean meal-based diet supplemented with 0.3% resveratrol. After a 7-day acclimatization period, all groups of yellow-feathered broiler chickens began the formal trial at 52 days of age and ended at 66 days of age. Starting at 52 days of age, the control group received intraperitoneal injections of normal saline (NS, 5 mg / kg body weight) for 5 consecutive days, while the other groups received intraperitoneal injections of dexamethasone (DEX, 5 mg / kg body weight) for 5 consecutive days, with injections discontinued on the sixth day. The experiment was conducted in the animal husbandry facilities of the College of Animal Science, South China Agricultural University, with free access to feed and water throughout the trial. The basal corn-soybean meal diet formula (hereinafter referred to as the diet) is shown in Table 1 and was formulated according to the nutritional standards recommended by the Ministry of Agriculture and Rural Affairs of China.

[0043] Table 1. Basic Corn-Soybean Meal Diet Formulations

[0044] Components per kilogram of premix: Vitamin A 11,200 IU; Vitamin D3 2,800 IU; Vitamin E 25 mg; Vitamin K3 2 mg; Vitamin B1 2 mg; Vitamin B2 8 mg; Vitamin B6 4 mg; Vitamin B12 0.02 mg; Pantothenic acid 20 mg; Niacin 50 mg; Folic acid 1 mg; Biotin 0.2 mg; Iron 60 mg; Copper 10 mg; Manganese 80 mg; Zinc 60 mg; Iodine 0.2 mg; Selenium 0.3 mg.

[0045] II. Sample Collection On days 1 (52 days old) and 15 (66 days old), chickens in each group were weighed and their daily feed intake was recorded. On days 6 (57 days old) and 15, two chickens with weights close to the average were selected from each replicate group for sampling. 10 mL of blood was collected from the jugular vein of each chicken, centrifuged at 3000 rpm for 10 minutes to obtain serum, and oxidation-related serum biochemical parameters were measured. Subsequently, the chickens were slaughtered, and tissue samples were collected from the pectoral muscle, leg muscle, liver, and segments of the duodenum, jejunum, and ileum. Some intestinal mucosal samples and molecular samples from the duodenum and ileum were stored at -80°C for subsequent analysis of antioxidant capacity, meat quality, intestinal morphology, and gene expression.

[0046] Example 2: Growth Performance Measurement I. Experimental Methods Using chickens from different treatment groups in Example 1 as samples, the feed consumption of each replicate group was recorded before each weighing or when the chickens died, and the average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / G) were calculated to evaluate growth performance.

[0047] II. Test Results The test results are as follows Figure 3 As shown, DEX treatment significantly reduced daily weight gain (ADG) in 52–66-day-old yellow-feathered broilers (P<0.05), but had no significant effect on daily feed intake (ADFI) during the same period. Simultaneously, DEX treatment significantly increased the feed conversion ratio (F / G) of 52–66-day-old yellow-feathered broilers (P<0.05). Adding 0.1% and 0.2% resveratrol (RES) to the diet significantly alleviated the negative impact of DEX on ADG in 52–66-day-old broilers (P<0.05) and significantly reduced the DEX-induced increase in F / G (P<0.05). Furthermore, no significant differences were observed between different RES levels.

[0048] Example 3 Meat quality determination I. Experimental Methods Chickens from different treatment groups in Example 1 were used as samples. After sample collection, the breast and leg muscle samples were left to stand at room temperature for 45 minutes before their brightness (L*), redness (a*), and yellowness (b*) were measured. Another portion of the muscle samples was stored at 4°C for 24 hours before the color values ​​were measured again. The pH values ​​of the breast and leg muscles were measured using a Mettler Toledo pH meter at 45 minutes post-slaughter and after 24 hours of storage at 4°C. After trimming, the muscle samples were initially weighed (W0) and then stored at 4°C for 24 hours. The surface moisture was then absorbed, and the samples were weighed again (W1). The water loss rate was calculated as: Water loss rate (%) = [(W0-W1) / W0] × 100. Shear force was measured using a muscle tenderness meter from Harbin Xiangfang Jinqiao Machinery Processing Plant. II. Test Results Meat quality test results as follows Figure 4 and Figure 5 As shown, the results indicated that in 66-day-old yellow-feathered broilers, DEX treatment significantly reduced the a* and b* values ​​of leg muscles and showed a trend of decreasing L* values, while significantly increasing the pH values ​​at 4 h and 24 h post-slaughter (P<0.05); in addition, it also significantly reduced the a*, L*, and b* values ​​of breast muscles (P<0.05).

[0049] Adding 0.1% RES to a basal corn-soybean meal diet significantly improved the abnormal changes in leg muscle b* value and pH at 45 minutes post-slaughter induced by DEX, and significantly alleviated the abnormalities in pectoral muscle a*, L*, and pH at 45 minutes post-slaughter (P<0.05). Simultaneously, 0.1% RES also significantly reduced leg muscle drip loss (P<0.05) and to some extent alleviated the abnormal changes in leg muscle a* (P =0.0959) and L* (P = 0.0935). 0.2% RES significantly improved the abnormalities in leg muscle L* value and pH at 45 minutes post-slaughter induced by DEX, as well as the abnormality in pectoral muscle L* value (P<0.05), and also significantly reduced leg muscle drip loss (P<0.05), and to some extent alleviated the abnormalities in leg muscle a* (P = 0.0574) and pectoral muscle pH at 45 minutes post-slaughter (P =0.0582). Meanwhile, the addition of 0.3% RES to the diet significantly improved the abnormalities in a* and b* values ​​and pH 45 minutes after slaughter caused by DEX, as well as the abnormalities in L* and b* values ​​and pH 45 minutes after slaughter in the pectoral muscle (P<0.05), significantly reduced drip loss in the leg muscle (P<0.05), and showed a trend of reducing pectoral muscle shear force (P = 0.0911).

[0050] Example 4: Determination of serum biochemical indicators I. Experimental Methods Serum corticosterone (CS) levels were measured using a radioimmunoassay kit provided by Wuhan Huamei Biotechnology Co., Ltd. Serum albumin (ALB), globulin (GLB), glucose (Glu), and total protein levels were measured using kits provided by Nanjing Jiancheng Bioengineering Research Institute. Serum creatinine (Cr) and blood urea nitrogen (BUN) levels were measured by Guangzhou Da'an Research Center using colorimetric and urease methods, respectively. Specific experimental methods were performed according to the kit instructions.

[0051] II. Test Results Serum biochemical index analysis results as follows Figure 6 As shown, the results indicated that in 57-day-old yellow-feathered broilers, DEX treatment significantly increased serum corticosterone (CS) levels (P<0.05). Simultaneously, the addition of 0.1%, 0.2%, and 0.3% RES to the diet significantly alleviated the DEX-induced increase in serum CS levels (P<0.05). At 66 days of age, DEX treatment significantly increased serum CS and blood urea nitrogen (BUN) levels (P<0.05). At this point, the addition of 0.1% RES to the diet significantly increased serum albumin (ALB) and decreased CS and BUN (P<0.05); the addition of 0.2% RES significantly increased serum ALB and total protein (TP) levels and decreased BUN (P<0.05); while the addition of 0.3% RES significantly increased serum TP levels and decreased CS and BUN (P<0.05).

[0052] Example 5: Determination of antioxidant capacity I. Experimental Methods Using commercially available kits provided by Nanjing Jiancheng Bioengineering Research Institute, the levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in plasma, leg muscle, and liver were determined; simultaneously, the levels of SOD and MDA in the duodenal, jejunal, and ileal mucosa were measured. Specific experimental methods were performed according to the kit instructions.

[0053] II. Test Results The results of the assessment of antioxidant capacity of serum and tissues are as follows: Figure 7 , Figure 8 , Figure 9 and Figure 10As shown, the results indicated that DEX treatment significantly increased the malondialdehyde (MDA) content in the liver and leg muscles of 66-day-old yellow-feathered broilers (P<0.05) and significantly decreased the activities of superoxide dismutase (SOD) in leg muscles and serum, while also decreasing the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in leg muscles (P<0.05). Under DEX treatment, the addition of 0.1% RES to the diet significantly increased serum GSH-Px, liver SOD and GSH-Px, as well as the activities of SOD, CAT, and GSH-Px in leg muscles (P<0.05). For 0.2% RES, under DEX treatment, it significantly increased the activities of serum SOD, liver SOD, and leg muscle SOD, CAT, and GSH-Px (P<0.05), while showing a trend of decreasing leg muscle MDA levels (P = 0.0517) and increasing serum GSH-Px activity (P = 0.0810). In addition, the addition of 0.3% RES to the diet significantly reduced the level of MDA in leg muscles under DEX treatment (P<0.05), and significantly increased the activities of SOD and GSH-Px in the liver and leg muscles (P<0.05). At the same time, it also increased the activities of GSH-Px in serum (P = 0.0786) and liver (P = 0.0806) and CAT in leg muscles to a certain extent (P = 0.0629).

[0054] Results of intestinal antioxidant capacity analysis as follows Figure 11 As shown, the results indicated that DEX treatment significantly increased the MDA content in the duodenum, jejunum, and ileum of 66-day-old yellow-feathered broilers (P<0.05). Based on this, dietary supplementation with 0.1% RES significantly reduced MDA levels in the jejunum and ileum under DEX treatment (P<0.05), and to some extent reduced duodenal MDA levels (P = 0.0832). For 0.2% RES, DEX treatment significantly reduced jejunal MDA (P<0.05), and to some extent reduced duodenal MDA (P = 0.0971) and increased duodenal SOD activity (P = 0.0821). Furthermore, dietary supplementation with 0.3% RES significantly reduced jejunal MDA levels and significantly increased duodenal SOD activity under DEX treatment (P<0.05).

[0055] Example 6 Intestinal Morphology I. Experimental Methods Samples approximately 2 cm long were taken from the mid-duodenal segment, the anterior 1 / 4 segment of the jejunum, and the mid-ileum. They were fixed in 4% paraformaldehyde phosphate buffer at pH 7.4 and sent to Beijing Jiputeng Biotechnology Co., Ltd. to prepare tissue sections with a thickness of 5 μm. The sections were observed under a 50x eyepiece, and the villus height and crypt depth were measured using Motic Advanced 3.0 software. The ratio of villus height to crypt depth was calculated.

[0056] II. Test Results Results of intestinal morphology analysis as follows Figure 14 and Figure 15 As shown, the results indicated that DEX treatment significantly reduced villus height in the duodenum and jejunum of 67-day-old broilers (P<0.05), and to some extent reduced villus height in the ileum (P =0.0976). Simultaneously, DEX significantly reduced the villus height to crypt depth ratio in the duodenum and jejunum (P<0.05).

[0057] Adding 0.1% and 0.3% RES to the diet significantly alleviated the decrease in ileal villus height induced by DEX (P<0.05), while 0.2% RES showed a certain degree of alleviation trend (P = 0.0929). Furthermore, adding 0.3% RES significantly increased the ratio of villus height to crypt depth in the ileum under DEX treatment (P<0.05), while 0.1% RES showed a trend of increasing this ratio (P = 0.0529).

[0058] Example 7: Relative mRNA Expression Level I. Experimental Methods Real-time quantitative PCR was performed using an ABI 7500 instrument and a SYBR Green kit. Specific primer sequences are shown in Table 2. Primer specificity and efficiency were verified using mixed samples; primers with efficiencies between 90% and 110% were used for experimental analysis. All reactions were repeated, with a difference of less than 5% between replicates considered acceptable. A control without reverse transcription and a control without template were included. PCR reaction conditions were: 95℃ pre-denaturation for 1 minute, 40 cycles (95℃ 15 seconds, 55-62℃ 30 seconds, 72℃ 45 seconds), followed by melting curve analysis (95℃ 30 seconds, 60℃ 30 seconds, 95℃ 30 seconds). Target gene expression levels were measured using a 2-1... -△△Ct The method was used to calculate the result, with GAPDH as the internal reference gene.

[0059] Table 2 Primer sequence listing

[0060] II. Test Results The results of the analysis of intestinal gene mRNA expression levels are as follows: Figure 14 and Figure 15 As shown, the results indicated that DEX treatment significantly reduced the expression of Ocludin mRNA in the duodenum of 66-day-old broilers (P<0.05) and significantly increased the expression of CAT mRNA in the duodenum, while also reducing the expression of ZO-1 mRNA in the ileum to some extent (P = 0.0657).

[0061] Compared with the DEX group, dietary supplementation with 0.1% RES significantly increased the expression of SOD and Octudin mRNA in the duodenum, and the expression of GPX2, CAT, PPAR-γ, and Octudin mRNA in the ileum (P<0.05), while decreasing the expression of CAT mRNA in the duodenum, and to some extent increasing the expression of ZO-1 mRNA in the ileum (P =0.0657). Similarly, 0.2% RES significantly increased the expression of Octudin mRNA in the duodenum, and the expression of SOD and Octudin mRNA in the ileum (P<0.05), while decreasing the expression of CAT mRNA in the duodenum (P<0.05), and showing a trend of increasing ZO-1 mRNA in the ileum (P = 0.0809). Finally, 0.3% RES significantly increased the expression of GPX2, SOD and Ocludin mRNA in the ileum (P<0.05), decreased the expression of CAT mRNA in the duodenum (P<0.05), and to some extent increased the expression of ZO-1 mRNA in the ileum (P = 0.0581).

[0062] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. Application of resveratrol in the preparation of products that improve oxidative stress in poultry.

2. Application of resveratrol in the preparation of feed to improve oxidative stress in poultry.

3. A feed, characterized in that, By weight, it contains the following components: 0.1-0.3 parts resveratrol, 70-77 parts energy feed, 19.5-29.2 parts protein feed, 0.1-0.2 parts premix, and 0.15-0.3 parts additives.

4. The feed according to claim 3, characterized in that, The energy feed includes corn and lard.

5. The feed according to claim 3, characterized in that, The protein feed is one or more of soybean meal, corn gluten meal, lysine, methionine, and threonine.

6. The feed according to claim 3, characterized in that, The mineral feed is one or more of the following: stone powder, dicalcium phosphate, and sodium chloride.

7. The feed according to claim 3, characterized in that, The additives are sodium bicarbonate and choline chloride.

8. The feed according to claim 3, characterized in that, The premix contains vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, pantothenic acid, niacin, folic acid, biotin, iron, copper, manganese, zinc, iodine, and selenium.

9. The feed according to any one of claims 3-8, characterized in that, By weight, it contains the following components: 0.1-0.3 parts resveratrol, 68-74 parts corn, 15-20 parts soybean meal, 4-8 parts corn gluten meal, 2-3 parts lard, 1-1.5 parts limestone powder, 0.5-1.5 parts dicalcium phosphate, 0.3-0.9 parts lysine, 0.2-0.3 parts salt, 0.15-0.2 parts methionine, 0.1-0.15 parts sodium bicarbonate, 0.05-0.15 parts choline chloride, 0.05-0.1 parts threonine, and 0.1-0.2 parts premix.

10. The use of the feed according to any one of claims 3-9 in the preparation of products that improve oxidative stress in poultry.

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