Premix for regulating gastrointestinal health and promoting growth of animals and preparation method thereof

Abstract

The application provides a premix for regulating gastrointestinal health of animals and promoting growth and a preparation method thereof, which comprises the following components: Chinese wolfberry polysaccharide, pueraria flavones and hawthorn flavones, bacillus subtilis, amylase, cellulase, xylanase and beta-glucanase, vitamin A, nicotinamide, ferric glycine, zinc sulfate and medical stone; through scientific compounding and functional synergy of Chinese wolfberry polysaccharide, pueraria flavones and hawthorn flavones, bacillus subtilis, complex enzyme and vitamins and minerals, the balance of intestinal flora can be regulated, the in-vitro pre-digestion and in-vivo digestion efficiency of feed can be significantly enhanced, and the intestinal wall health and nutrient absorption can be promoted, so that the integrated effect of'regulating intestines, digesting food and promoting absorption' is realized, so that the digestion rate of animals to crude fiber and starch can be significantly improved, the incidence of diarrhea can be reduced, the daily weight gain of animals can be improved, the feed reward can be improved, the feed utilization rate can be improved, and high economic benefits can be obtained.

Description

Technical Field

[0001] This invention relates to the field of animal feed additives, specifically to a premix that regulates animal gastrointestinal health and promotes growth, and its preparation method. Background Technology

[0002] Animals’ high-efficiency production depends heavily on gastrointestinal health and efficient fermentation. However, in actual production, stress, feed changes, and high-concentrate diets often lead to digestive disorders in animals, manifested as abnormal feed fermentation, insufficient digestive enzyme activity, nutrient absorption disorders, intestinal flora imbalance and diarrhea. These problems directly lead to feed waste, slow growth, decreased production performance and economic losses. The main ways to solve these problems are: (1) using single microecological preparations such as yeast and Bacillus, which mainly focus on regulating the balance of flora and have limited direct effects on the protection of exogenous enzyme activity and the absorption of mineral nutrients; (2) using single enzyme preparations such as cellulase and amylase to supplement the deficiency of endogenous enzymes and improve the substrate degradation rate. However, enzymes are easily inactivated in the complex environment of the gastrointestinal tract, and the effect is limited by the pH and microbial state in the gastrointestinal tract. Moreover, there is a lack of synergistic protection between enzymes and bacteria, and enzyme activity is easily reduced in the acidic environment of the gastrointestinal tract; (3) using nutritional supplements such as vitamins or trace elements. However, there is currently a lack of synergistic design for the digestion and absorption process, resulting in low overall efficiency, unstable effect and low cost-effectiveness.

[0003] Therefore, there is an urgent need to develop a premix that can integrate the multiple functions of bacteria, enzymes, and nutrients, and achieve synergistic effects through scientific formulation, thereby regulating the gastrointestinal health of animals and promoting digestion and absorption. Summary of the Invention

[0004] To address the aforementioned deficiencies in the prior art, this invention provides a premix for regulating animal gastrointestinal health and promoting growth, along with its preparation method. Through the scientific compounding and synergistic effects of wolfberry polysaccharides, kudzu root flavonoids, hawthorn flavonoids, Bacillus subtilis, complex enzymes, vitamins, and minerals, it can regulate the balance of intestinal flora, significantly enhance the efficiency of in vitro pre-digestion and in vivo digestion of feed, and promote intestinal wall health and nutrient absorption. This achieves an integrated effect of "soothing the intestines, aiding digestion, and promoting absorption," thereby significantly improving the digestibility of crude fiber and starch in animals, reducing the incidence of diarrhea, increasing daily weight gain or milk production, and improving feed efficiency, resulting in high economic benefits. The prepared premix can be used in animal feed or as an animal feed additive for cattle, pigs, chickens, and other animals.

[0005] To achieve the above technical effects, the following technical solution is adopted: A premix for regulating gastrointestinal health and promoting growth in animals comprises the following components: 15-30 parts of Lycium barbarum polysaccharide, 13-25 parts of plant flavonoids, 8-12 parts of Bacillus subtilis, 3-6 parts of amylase, 2-3 parts of cellulase, 10-18 parts of compound enzyme, 0.04-0.06 parts of vitamin A, 0.04-0.06 parts of nicotinamide, 0.15-0.3 parts of iron glycine, 0.15-0.3 parts of zinc sulfate, and 50-70 parts of maifanite. Furthermore, the premix comprises the following components: 18-23 parts of wolfberry polysaccharide, 15-20 parts of plant flavonoids, 9-11 parts of Bacillus subtilis, 4-5 parts of amylase, 2-3 parts of cellulase, 13-16 parts of compound enzyme, 0.05 parts of vitamin A, 0.05 parts of nicotinamide, 0.2 parts of glycine iron, 0.2 parts of zinc sulfate, and 60-70 parts of maifanite. Furthermore, the plant flavonoids include 8-15 parts of kudzu root flavonoids and 5-10 parts of hawthorn flavonoids; Furthermore, the complex enzyme comprises 4-8 parts xylanase and 6-10 parts β-glucanase; This invention also provides a method for preparing the above-mentioned premix for regulating animal gastrointestinal health and promoting growth, comprising the following steps: Step S1: Prepare the mixture of plant flavonoids and the compound enzyme separately for later use; Step S2: Premix vitamin A, nicotinamide, glycine iron, zinc sulfate and maifanite carrier to prepare premix A; Step S3: Mix Bacillus subtilis freeze-dried powder with maifanite carrier to prepare premix B; Step S4: Mix the plant flavonoid mixture prepared in step S1 with the compound enzyme, wolfberry polysaccharide, amylase, cellulase, premix A and premix B and the remaining maifanite in proportion to obtain the premix that regulates animal gastrointestinal health and promotes growth. Furthermore, in step S1, the preparation method of the plant flavonoid mixture is as follows: weigh kudzu root flavonoids and hawthorn flavonoids and mix them evenly in a certain proportion to obtain the plant flavonoid mixture; Furthermore, in step S1, the preparation method of the composite enzyme is as follows: weigh xylanase and β-glucanase and mix them evenly in a certain proportion to obtain the composite enzyme; Furthermore, in step S4, the plant flavonoid mixture, compound enzyme, wolfberry polysaccharide, amylase, cellulase, premix A and premix B, and the remaining maifanite are placed in a vertical mixer in proportion and mixed for 3-8 minutes. Furthermore, the uniformity of the premix prepared in step S4 for regulating animal gastrointestinal health and promoting growth is tested, and the sample is then packaged after passing the test. The present invention also provides the application of the above-mentioned premix for regulating animal gastrointestinal health and promoting growth, or the premix for regulating animal gastrointestinal health and promoting growth prepared by the above-mentioned preparation method, in feed or feed additives.

[0006] The beneficial effects of this invention are as follows: This invention provides a premix for regulating animal gastrointestinal health and promoting growth, and its preparation method. Through the scientific compounding and synergistic effects of wolfberry polysaccharides, kudzu root flavonoids, hawthorn flavonoids, Bacillus subtilis, compound enzymes, vitamins, and minerals, it can regulate the balance of intestinal flora, significantly enhance the efficiency of in vitro pre-digestion and in vivo digestion of feed, and promote intestinal wall health and nutrient absorption, thereby achieving an integrated effect of "soothing the intestines, aiding digestion, and promoting absorption." This significantly improves the digestibility of crude fiber and starch in animals, reduces the incidence of diarrhea, increases daily weight gain, improves feed efficiency, and enhances feed utilization efficiency, resulting in high economic benefits. The prepared premix can be used in animal feed or as an animal feed additive for cattle, pigs, chickens, etc. Its mechanism of action is as follows: Kudzu root flavonoids and hawthorn flavonoids can synergistically regulate the structure of the intestinal flora in animals, and the two can synergistically inhibit the proliferation of harmful bacteria and promote the colonization of beneficial bacteria. The plant extracts work through a synergistic pathway of "microbial regulation - anti-oxidation and anti-inflammation - digestive enzyme activation - mucosal barrier protection," thereby strengthening the intestinal mucosal barrier function and synergistically improving feed digestibility and nutrient transport efficiency. Lycium barbarum polysaccharides can improve animal stress and provide auxiliary relief for digestive disorders, regulate stress hormone levels, enhance antioxidant and immune capabilities, protect the intestinal mucosal barrier, regulate intestinal microbial balance, and improve feed digestibility. Lycium barbarum polysaccharides can activate the intestinal mucosal immune system, synergistically with the anti-inflammatory effects of kudzu root flavonoids and hawthorn flavonoids, reducing the impact of intestinal damage on digestion and absorption. Simultaneously, Lycium barbarum polysaccharides can encapsulate flavonoid molecules, reducing their degradation in the gastrointestinal tract, increasing flavonoid bioavailability, and prolonging their duration of action in the intestine. Furthermore, Lycium barbarum polysaccharides can promote the absorption of flavonoids by intestinal epithelial cells and enhance the regulatory effect of flavonoids on nutrient transporters. Bacillus subtilis, through its metabolites and the bacterial cells themselves, can effectively protect exogenous enzyme preparations, reduce the inhibition and damage to enzyme activity caused by the complex gastrointestinal environment, and ensure enzyme effectiveness. The complex enzyme can improve feed digestibility, and its degradation products, in turn, act as prebiotics, promoting the proliferation and colonization of Bacillus subtilis. Nicotinamide, as a component of coenzymes I and II, can directly participate in energy metabolism in the body and improve carbohydrate utilization. Organic trace elements such as glycine iron and zinc sulfate have higher absorption rates in the intestine, effectively preventing anemia and enhancing immunity. Vitamin A mainly functions to maintain the integrity and health of intestinal mucosal epithelial cells, while maifanite can adsorb toxins, further maintaining intestinal health. Detailed Implementation

[0007] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0008] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0009] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments of the present invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, and / or combinations thereof.

[0010] In the following examples, unless otherwise specified, all reagents and consumables were purchased from conventional reagent manufacturers in the art; unless otherwise specified, the experimental methods and techniques used are conventional methods and techniques in the art.

[0011] In the following examples and comparative examples: Lycium barbarum polysaccharide was purchased from Shaanxi Huabize Bioengineering Co., Ltd., catalog number HBZ-GQDT, purity 90%; Pueraria lobata flavonoids were purchased from Shaanxi Jiuenkang Biotechnology Co., Ltd., catalog number JEK-358, purity 80%; Hawthorn flavonoids were purchased from Changsha Shenghe Bioengineering Co., Ltd., catalog number shsww, purity 80%; Astragalus membranaceus polysaccharide was purchased from Xi'an Feida Biotechnology, catalog number FDBL-021; Bacillus subtilis was purchased from Shandong Tianrunhe Bioengineering Co., Ltd. Product code: KGS251013; Bacillus licheniformis was purchased from Shanxi Jiuding Biotechnology Co., Ltd., product code: JD-2025102001; amylase, cellulase, xylanase and β-glucanase were all purchased from Baiyin Sainuo Biotechnology Co., Ltd., product code: 20250927ZD; cellulase product code: 20250929XW; xylanase product code: 20250920XP; β-glucanase product code: 20250920BP.

[0012] Example 1: A premix for regulating gastrointestinal health and promoting growth in animals comprises the following components: 20 parts of Lycium barbarum polysaccharide, 10 parts of Pueraria lobata flavonoids, 8 parts of Hawthorn flavonoids, 10 parts of Bacillus subtilis, 5 parts of amylase, 3 parts of cellulase, 6 parts of xylanase, 8 parts of β-glucanase, 0.05 parts of vitamin A (500,000 IU / g), 0.05 parts of nicotinamide, 0.2 parts of glycine iron (calculated as Fe, w=17.0%), 0.2 parts of zinc sulfate (calculated as Zn, w=34.5%, monohydrate), and 67 parts of maifanite. The method for preparing the premix for regulating animal gastrointestinal health and promoting growth includes the following steps: Step S1: Weigh 10 parts of kudzu flavonoids and 8 parts of hawthorn flavonoids, mix them evenly to obtain a flavonoid mixture; weigh 6 parts of xylanase and 8 parts of β-glucanase, mix them evenly to obtain a complex enzyme; Step S2: Weigh 0.05 parts of vitamin A, 0.05 parts of nicotinamide, 0.2 parts of iron glycine, and 0.2 parts of zinc sulfate, and premix them with 2 parts of maifanite carrier to prepare premix A; Step S3: Weigh 10 parts of Bacillus subtilis, mix Bacillus subtilis with 10 parts of Maifan stone carrier to prepare premix B; Step S4: Weigh 20 parts of wolfberry polysaccharide, 5 parts of amylase, 3 parts of cellulase, and 55 parts of maifanite. Place the above components, the flavonoid mixture and compound enzyme prepared in step S1, as well as premix A and premix B, into a vertical mixer and mix for 5 minutes. The vertical mixer has a power of 30KW and a speed of 40rpm. After mixing evenly, the premix SY1, which regulates the gastrointestinal health of animals and promotes growth, is obtained.

[0013] Example 2 A premix for regulating gastrointestinal health and promoting growth in animals comprises the following components: 30 parts of wolfberry polysaccharide, 15 parts of kudzu root flavonoids, 10 parts of hawthorn flavonoids, 12 parts of Bacillus subtilis, 6 parts of amylase, 3 parts of cellulase, 8 parts of xylanase, 10 parts of β-glucanase, 0.06 parts of vitamin A, 0.06 parts of nicotinamide, 0.3 parts of glycine iron (calculated as Fe, w=17.0%), 0.3 parts of zinc sulfate (calculated as Zn, w=34.5%, monohydrate), and 70 parts of wheat bran. The method for preparing the premix for regulating animal gastrointestinal health and promoting growth includes the following steps: Step S1: Weigh 15 parts of kudzu flavonoids and 10 parts of hawthorn flavonoids, mix them evenly to obtain a flavonoid mixture; weigh 8 parts of xylanase and 10 parts of β-glucanase, mix them evenly to obtain a complex enzyme. Step S2: Weigh 0.06 parts of vitamin A, 0.06 parts of nicotinamide, 0.3 parts of iron glycine, and 0.3 parts of zinc sulfate, and premix them with 2 parts of maifanite carrier to prepare premix A; Step S3: Weigh 12 portions of Bacillus subtilis, mix Bacillus subtilis with 12 portions of maifanite carrier to prepare premix B; Step S4: Weigh 30 parts of wolfberry polysaccharide, 6 parts of amylase, 3 parts of cellulase, and 56 parts of maifanite. Place the above components, the flavonoid mixture and compound enzyme prepared in step S1, as well as premix A and premix B, into a vertical mixer and mix for 5 minutes. The vertical mixer has a power of 30KW and a speed of 40rpm. After mixing evenly, premix SY2, which regulates the gastrointestinal health of animals and promotes growth, is obtained.

[0014] Example 3 A premix for regulating gastrointestinal health and promoting growth in animals comprises the following components: 15 parts of Lycium barbarum polysaccharide, 8 parts of Pueraria lobata flavonoids, 5 parts of Hawthorn flavonoids, 8 parts of Bacillus subtilis, 3 parts of amylase, 2 parts of cellulase, 4 parts of xylanase, 6 parts of β-glucanase, 0.04 parts of vitamin A, 0.04 parts of nicotinamide, 0.2 parts of glycine iron (calculated as Fe, w=17.0%), 0.2 parts of zinc sulfate (calculated as Zn, w=34.5%, monohydrate), and 50 parts of maifanite. The method for preparing the premix for regulating animal gastrointestinal health and promoting growth includes the following steps: Step S1: Weigh 8 parts of kudzu flavonoids and 5 parts of hawthorn flavonoids, mix them evenly to obtain a flavonoid mixture; weigh 4 parts of xylanase and 6 parts of β-glucanase, mix them evenly to obtain a complex enzyme; Step S2: Weigh 0.04 parts of vitamin A, 0.04 parts of nicotinamide, 0.2 parts of iron glycine, and 0.2 parts of zinc sulfate, and premix them with 2 parts of maifanite carrier to prepare premix A; Step S3: Weigh 8 portions of Bacillus subtilis, mix Bacillus subtilis with 8 portions of maifanite carrier, and prepare premix B; Step S4: Weigh 15 parts of wolfberry polysaccharide, 3 parts of amylase, 2 parts of cellulase, and 40 parts of maifanite. Place the above components, the flavonoid mixture and compound enzyme prepared in step S1, as well as premix A and premix B, into a vertical mixer and mix for 5 minutes. The vertical mixer has a power of 30 KW and a speed of 40 rpm. After mixing evenly, the premix SY3, which regulates the gastrointestinal health of animals and promotes growth, is obtained.

[0015] Comparative Example 1 Based on Example 1, without adding wolfberry polysaccharide, 20 parts by weight of kudzu root flavonoids, and 18 parts by weight of hawthorn flavonoids (see Table 1 for details), the other components and process conditions are the same as in Example 1, and premix DY1 is obtained.

[0016] Comparative Example 2 Based on Example 1, without adding kudzu flavonoids, the weight parts of hawthorn flavonoids were 18 parts (see Table 1 for details). Other components and process conditions were the same as in Example 1, resulting in premix DY2.

[0017] Comparative Example 3 Based on Example 1, without adding hawthorn flavonoids, the weight parts of kudzu flavonoids were 18 parts (see Table 1 for details). Other components and process conditions were the same as in Example 1, resulting in premix DY3.

[0018] Comparative Example 4 Based on Example 1, without adding kudzu root flavonoids and hawthorn flavonoids, the weight parts of wolfberry polysaccharide were 38 parts (see Table 1 for details). Other components and process conditions were the same as in Example 1, resulting in premix DY4.

[0019] Comparative Example 5 Based on Example 1, Astragalus polysaccharide was used instead of Lycium barbarum polysaccharide (see Table 1 for specific groups), and other components and process conditions were the same as in Example 1 to obtain premix DY5.

[0020] Comparative Example 6 Based on Example 1, Bacillus licheniformis was used instead of Bacillus subtilis (see Table 1 for details), and the other components and process conditions were the same as in Example 1 to obtain premix DY6.

[0021] Comparative Example 7 Based on Example 1, without adding β-glucanase, the amount of amylase added was 8 parts, cellulase 5 parts, and xylanase 9 parts (see Table 1 for details). Other components and process conditions were the same as in Example 1, resulting in premix DY7.

[0022] Comparative Example 8 Based on Example 1, xylanase was not added, but amylase was added in the amount of 7 parts, cellulase in the amount of 5 parts, and β-glucanase in the amount of 10 parts (see Table 1 for specific components and their amounts). Other components and process conditions were the same as in Example 1, and premix DY8 was obtained.

[0023] Table 1. Composition of premixes in Comparative Examples 1-8

[0024] Test case Take 0.2 wt% of the premix from Examples 1-3 and Comparative Examples 1-8 respectively, and mix it with 65.8 wt% corn, 5 wt% soybean meal, 15 wt% rapeseed meal, 10 wt% DDGS (corn ethanol grains), and 4 wt% corn ethanol. wt% beef cattle fattening premix (purchased from Henan Dabeinong Feed Co., Ltd.) was mixed to prepare concentrates G1-G11; 4 kg of concentrates G1-G11 were mixed with 5 kg of corn silage, 2 kg of rice straw, and 5 kg of distiller's grains to prepare total diets DG1-DG11 respectively; 360 Simmental crossbred cattle of similar weight and good condition were selected and randomly divided into 12 groups of 30 cattle each; the experiment lasted for 70 days, including a 10-day pre-feeding period and a 60-day regular feeding period, with free access to feed; a total of 12 experimental groups were set up, and the total diets DG1-DG11 prepared using the premixes from Examples 1-3 and Comparative Examples 1-8 were fed respectively. The blank control group did not add the premixes from the examples and comparative examples to its total diet; the feeding conditions of the total diets for different groups are shown in Table 2.

[0025] Table 2. Feeding details of total rations for different groups

[0026] Trial Management The experimental cattle were housed in separate pens with natural ventilation. They were fed at 7:00 AM and 3:00 PM daily, with free access to fodder and clean drinking water. The cattle pens were regularly cleaned and disinfected to maintain hygiene. Before the experiment, the cattle were strictly dewormed and vaccinated according to the immunization schedule, and the feeding and management conditions were basically the same across all groups.

[0027] Test Records Growth performance: The experimental cattle were weighed on an empty stomach before early feeding. Initial body weight, final body weight, daily feed intake, and feed conversion ratio were measured.

[0028] Determination of the digestibility of major nutrients in the diet: The digestibility of starch, crude fiber, and dry matter was determined using an exogenous indicator method. The indicator Cr2O3 was uniformly mixed into the feed at a specific ratio and fed to the animals using standard methods. During the stabilization period of the digestibility test, representative fecal samples were continuously collected, and the concentrations of nutrients and indicators in the feed and feces were determined separately.

[0029] Nutrient digestibility = 1 - (indicator concentration in feed × nutrient concentration in feces) / (indicator concentration in feces × nutrient concentration in feed).

[0030] Table 3 shows the growth, feed intake, feed conversion ratio, and digestion and absorption of the experimental cattle in experimental groups 1-3 and comparative experimental groups 1-8.

[0031] Table 3. Growth, feed intake, feed conversion ratio, and digestion and absorption of the experimental cattle.

[0032] Table 3 shows that the experimental cattle in experimental groups 1-3 had the highest daily weight gain and the lowest feed conversion ratio, indicating that the feed utilization rate was highest when the premixes SY1-SY3 prepared in Examples 1-3 were used as a complete diet additive. Furthermore, the dry matter digestibility, starch digestibility, and crude fiber digestibility of the experimental cattle in experimental groups 1-3 were significantly improved compared to the blank control group, and significantly higher than all control experimental groups, indicating that the feed conversion rate was highest when the premixes SY1-SY3 prepared in Examples 1-3 were used as a complete diet additive. Among all test indicators, experimental groups 1-3 showed the best performance, significantly better than control experimental groups 1-8. This invention, through the scientific compounding and functional synergy of Lycium barbarum polysaccharides, Pueraria lobata flavonoids, hawthorn flavonoids, Bacillus subtilis, compound enzymes, vitamins, and minerals, can regulate the balance of intestinal flora, stabilize the intestinal flora, significantly enhance the efficiency of in vitro pre-digestion and in vivo digestion of feed, and promote intestinal wall health and nutrient absorption, thereby achieving an integrated effect of "soothing the intestines, aiding digestion, and promoting absorption." The premix provided by this invention can significantly improve the digestibility of crude fiber and starch in animals, reduce the incidence of diarrhea, increase daily weight gain, improve feed efficiency, and increase feed utilization, thus having high economic benefits.

[0033] Therefore, those skilled in the art will recognize that although embodiments of the present invention have been shown and described in detail herein, many other variations or modifications conforming to the principles of the present invention can be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Therefore, the scope of the present invention should be understood and recognized as covering all such other variations or modifications.

Claims

1. A premix for regulating gastrointestinal health and promoting growth in animals, characterized in that, It includes the following components: 15-30 parts of wolfberry polysaccharide, 13-25 parts of plant flavonoids, 8-12 parts of Bacillus subtilis, 3-6 parts of amylase, 2-3 parts of cellulase, 10-18 parts of compound enzyme, 0.04-0.06 parts of vitamin A, 0.04-0.06 parts of nicotinamide, 0.15-0.3 parts of iron glycine, 0.15-0.3 parts of zinc sulfate, and 50-70 parts of maifanite.

2. The premix for regulating animal gastrointestinal health and promoting growth as described in claim 1, characterized in that, It includes the following components: 18-23 parts of wolfberry polysaccharide, 15-20 parts of plant flavonoids, 9-11 parts of Bacillus subtilis, 4-5 parts of amylase, 2-3 parts of cellulase, 13-16 parts of compound enzyme, 0.05 parts of vitamin A, 0.05 parts of nicotinamide, 0.2 parts of iron glycine, 0.2 parts of zinc sulfate, and 60-70 parts of maifanite.

3. The premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claim 1, characterized in that, The plant flavonoids include 8-15 parts of kudzu root flavonoids and 5-10 parts of hawthorn flavonoids.

4. The premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claim 1, characterized in that, The complex enzyme comprises 4-8 parts xylanase and 6-10 parts β-glucanase.

5. A method for preparing a premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claims 1-4, characterized in that, Includes the following steps: Step S1: Prepare the mixture of plant flavonoids and the compound enzyme separately for later use; Step S2: Premix vitamin A, nicotinamide, glycine iron, zinc sulfate and maifanite carrier to prepare premix A; Step S3: Mix Bacillus subtilis freeze-dried powder with maifanite carrier to prepare premix B; Step S4: Mix the plant flavonoid mixture prepared in step S1 with the compound enzyme, wolfberry polysaccharide, amylase, cellulase, premix A and premix B, and the remaining maifanite in a certain proportion to obtain the premix that regulates animal gastrointestinal health and promotes growth.

6. The method for preparing a premix for regulating animal gastrointestinal health and promoting growth as described in claim 5, characterized in that, In step S1, the preparation method of the plant flavonoid mixture is as follows: weigh kudzu root flavonoids and hawthorn flavonoids and mix them evenly in proportion to obtain the plant flavonoid mixture.

7. The method for preparing a premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claim 5, characterized in that, In step S1, the preparation method of the complex enzyme is as follows: weigh xylanase and β-glucanase, mix them evenly in a certain proportion, and then prepare the complex enzyme.

8. The method for preparing a premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claim 5, characterized in that, In step S4, the plant flavonoid mixture, compound enzyme, wolfberry polysaccharide, amylase, cellulase, premix A and premix B, and the remaining maifanite are placed in a vertical mixer and mixed for 3-8 minutes.

9. The method for preparing a premixed feed for regulating gastrointestinal health and promoting growth in animals as described in claim 5, characterized in that, The uniformity of the premix prepared in step S4 for regulating animal gastrointestinal health and promoting growth was tested, and the samples were packaged after passing the test.

10. The application of the premix for regulating animal gastrointestinal health and promoting growth as described in claims 1-4, or the premix for regulating animal gastrointestinal health and promoting growth prepared by the preparation method described in claims 5-9, in feed or feed additives.

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