Application of selenium and vitamin E in improving growth and gastrointestinal performance of meat sheep

Abstract

The application discloses application of yeast selenium and vitamin E in improving growth and gastrointestinal performance of mutton sheep, and relates to the technical field of animal feeding. The yeast selenium and vitamin E are added in a basic daily ration of mutton sheep, and the two can effectively improve the growth capacity of the mutton sheep and improve the gastrointestinal performance of the mutton sheep, so that the product quality of the mutton sheep is comprehensively improved.

Description

Technical Field

[0001] This invention relates to the field of animal husbandry technology, specifically to the application of yeast selenium and vitamin E in synergistic effects on improving the growth and gastrointestinal function of meat sheep. Background Technology

[0002] Livestock products, as an important component of the diet, provide people with a rich source of nutrition. With the improvement of living standards and the increasing awareness of health, people are paying more attention to the source, breeding methods, and processing of livestock products. Therefore, the structure of the livestock industry is constantly being optimized and adjusted, shifting from the past small-scale, scattered farming model to large-scale breeding to meet the current demand for high-quality and diversified products. Some antibiotic-based feed additives used in the past, while inhibiting pathogens, also suppressed the normal flora in the animal's digestive tract, disrupting the microecological balance; moreover, some antibiotics are excreted into the environment through animal metabolism, causing pollution. Therefore, it is necessary to find alternative feed additives to improve the growth capacity and gastrointestinal performance of farmed animals, especially meat sheep. Summary of the Invention

[0003] The purpose of this invention is to provide an application of yeast selenium and vitamin E in synergistically improving the growth and gastrointestinal function of meat sheep.

[0004] Another objective of this invention is to provide a total mixed pelleted feed for mutton sheep, which, by adding yeast selenium and vitamin E to the basal diet formula of mutton sheep, can effectively improve the growth capacity and gastrointestinal function of mutton sheep through synergistic effects, thereby comprehensively improving the quality of mutton sheep products.

[0005] The technical solution of the present invention is as follows: On the one hand, embodiments of the present invention provide the application of yeast selenium and vitamin E in synergistically improving the growth and gastrointestinal function of meat sheep.

[0006] Furthermore, in some embodiments of the present invention, by adding yeast selenium and vitamin E to the basic feed formula for mutton sheep, a total mixed pellet feed is prepared for feeding, thereby improving the growth capacity, rumen fermentation, and intestinal fermentation performance of mutton sheep; wherein, the amount of yeast selenium added is 0.6 mg / kg, and the amount of vitamin E added is 200 IU / kg, and the amount of addition refers to the level of addition per kilogram of basic feed for mutton sheep.

[0007] On the other hand, the present invention also provides a total mixed pelleted feed for mutton sheep, the raw materials of which include yeast selenium, vitamin E, and a basal diet; wherein the amount of yeast selenium added is 0.6 mg / kg, the amount of vitamin E added is 200 IU / kg, and the amount of addition refers to the level of addition per kilogram of basal diet.

[0008] Furthermore, in some embodiments of the present invention, the raw materials of the above-mentioned basic diet include corn, corn middlings, soybean meal, corn stalk pellets, sunflower seed shells, sprayed corn husks, dried corn lees, pumpkin seed shells, limestone powder, sodium bicarbonate, and premix.

[0009] Furthermore, in some embodiments of the present invention, the stone powder in the above-mentioned basal diet has a particle size of 40 mesh.

[0010] Furthermore, in some embodiments of the present invention, the raw material components of the above-mentioned basic diet, by mass percentage, include: 32.04% corn, 6% corn middlings, 6% soybean meal, 14% corn stalk pellets, 12.2% sunflower seed shells, 8% sprayed corn husks, 5% dried corn lees, 10% pumpkin seed shells, 2.16% limestone powder, 0.6% sodium bicarbonate, and 4% premix.

[0011] Furthermore, in some embodiments of the present invention, the premix is ​​composed of the following components: VA 10,000 IU, VD3 1,000 IU, Fe 32 mg, Cu 5 mg, Zn 18.5 mg, Mn 10 mg, I 0.5 mg, and Co 0.1 mg.

[0012] Compared with the prior art, the embodiments of the present invention have at least the following advantages or beneficial effects: To address the above aspects, embodiments of the present invention provide an application of the synergistic effect of yeast selenium and vitamin E in improving the growth and gastrointestinal performance of meat sheep, and provide a total mixed pelleted feed for meat sheep fortified with yeast selenium and vitamin E. Feeding ruminants, especially Sahu sheep, with this feed can improve the gastrointestinal performance of Sahu sheep under the synergistic effect of yeast selenium and vitamin E, particularly having a positive impact on their rumen fermentation and intestinal fermentation capacity, and promoting their growth and development. Detailed Implementation

[0013] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0014] It should be noted that the term "comprising," or any other variation thereof, is intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes it.

[0015] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0016] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0017] Example 1 This invention provides a total mixed pelleted feed for meat sheep, the raw materials of which are yeast selenium, vitamin E, and basal diet; The raw material components of the above-mentioned basic diet, by weight percentage, are as follows: corn 32.04%, corn middlings 6%, soybean meal 6%, corn stalk pellets 14%, sunflower seed shells 12.2%, sprayed corn husks 8%, dried corn lees 5%, pumpkin seed shells 10%, limestone powder 2.16%, sodium bicarbonate 0.6%, and premix 4%.

[0018] The premixed material is composed of the following components: VA 10,000 IU, VD3 1,000 IU, Fe 32 mg, Cu 5 mg, Zn 18.5 mg, Mn 10 mg, I 0.5 mg, and Co 0.1 mg.

[0019] The addition amount of yeast selenium was 0.6 mg / kg, and the addition amount of vitamin E was 200 IU / kg. The addition amount refers to the addition level per kilogram of basal diet.

[0020] Comparative Example 1 This comparative example is based on Example 1. The difference from Example 1 is that yeast selenium and vitamin E are not added, and the product feed is only a basic diet. The composition of the basic diet is the same as that of Example 1.

[0021] Comparative Example 2 This comparative example is based on Example 1. The difference between this example and Example 1 is that only yeast selenium is added, and vitamin E is not added. The yeast selenium and the basic diet components are the same as in Example 1.

[0022] Comparative Example 3 This comparative example is based on Example 1. The difference between this example and Example 1 is that only vitamin E is added, and yeast selenium is not added. The vitamin E and the basic diet components are the same as in Example 1.

[0023] Test case In this experiment, yeast selenium was purchased from the Chaoquan Town Industrial Park, Feicheng City, Tai'an City, Shandong Province, with a purity ≥0.2%; vitamin E was purchased from Guangdong Huayue Animal Health Co., Ltd., with a purity ≥98%.

[0024] The experimental animals were two-month-old male Suffolk sheep lambs (a superior combination of Suffolk and Hu sheep).

[0025] Twenty-four healthy male Sahu lambs, aged 2 months and weighing (23.14±2.5 kg), were selected and randomly divided into four groups of six, with each group housed separately.

[0026] The four groups were fed the product feed provided by Comparative Example 1 (blank control group / Con group), Comparative Example 2 (yeast selenium group / SY group), Comparative Example 3 (vitamin E group / VE group), and Example 1 (yeast selenium and vitamin E mixed group / MIX group), respectively. The pre-feeding period was 10 days, and the formal feeding period was 60 days. The composition and nutritional components of the basal diet (comparative example 1) are shown in Table 1.

[0027] Table 1

[0028] Before the experiment began, the sheep were numbered, dewormed, and grouped into pens, and fed by designated personnel. They were fed twice daily, at 7:00 AM and 5:00 PM, and had free access to water. The pens were cleaned regularly to maintain cleanliness, and were disinfected and immunized periodically according to the farm's requirements.

[0029] Experimental data were organized using Excel 2021 software and analyzed using SPSS 22.0 using one-way ANOVA. Experimental data are expressed as mean ± standard deviation. P <0.05 indicates a significant difference between groups.

[0030] Growth performance testing: Lambs were weighed on an empty stomach before morning feeding on days 0, 30, and 60 of the formal period, and the weight was recorded. The average daily weight gain of each lamb was calculated. During the experiment, the amount of feed given and the amount of feed remaining for each group of lambs were recorded, the average daily feed intake of each lamb was calculated, and the feed conversion ratio was calculated based on the average daily weight gain and average daily feed intake.

[0031] The results are shown in Table 2.

[0032] Table 2

[0033] The results show that adding vitamin E can significantly increase the average daily weight gain of meat sheep. P <0.05).

[0034] Gastrointestinal performance testing: pH was measured using a Leici PHS-25 pH meter; NH3-N was measured using a colorimetric method. Solution A and solution B were prepared first, and a standard series of ammonium chloride ranging from 0 to 1.2 mg / 100 mL was prepared. 0.4 mL of each standard solution was added to 2 mL of solution A and 2 mL of solution B, and after standing for 10 min, the solution was measured at a wavelength of 700 nm (Solution A: 0.08 g of sodium nitroferricyanide was dissolved in 100 mL of 14% sodium salicylate solution; Solution B: 2 mL of sodium hypochlorite solution was measured). VFA was determined using an Agilent 7890B gas chromatograph. Samples were centrifuged, acidified with metaphosphoric acid, and filtered before injection. A DB-WAX capillary column (60m × 250μm × 0.25μm) was used with a programmed temperature increase: initial temperature of 100℃ for 0.5 min, increased at 8℃ / min to 180℃ for 1 min, then increased at 20℃ / min to 200℃ for 5 min. The injection port temperature was 250℃, the split ratio was 10:1, and the injection volume was 1 μL. The detector temperature was 250℃, and the flow rates of air, hydrogen, and make-up gas were 400, 30, and 25 mL / min, respectively. Helium was used as the carrier gas. Qualitative and quantitative analysis was performed using an internal standard curve (with the concentration ratio of each component to the internal standard as the x-axis and the peak area ratio as the y-axis).

[0035] The results are shown in Table 3 (rumen fermentation parameters) and Table 4 (gut fermentation parameters), respectively.

[0036] Table 3

[0037] As shown in Table 3, the pH values ​​of the SY and VE groups were significantly higher than those of the CON and MIX groups. P <0.05); the NH3-N in the MIX group was significantly lower than that in the CON and SY groups ( P <0.05); the contents of acetic acid, propionic acid, and total VFA in the MIX group were significantly higher than those in the other three groups ( P <0.05), and no significant differences were found in any of the other indicators ( P <0.05).

[0038] Table 4

[0039] As shown in Table 4, in the duodenum, the pH of the VE group was significantly lower than that of the other three groups. P <0.05), the NH3-N content in the MIX group was significantly higher than that in the CON and VE groups ( P<0.05), the isovaleric acid content in group CON was significantly higher than that in the other three groups ( P <0.05); In the jejunum, the NH3-N content in the MIX group was significantly higher than that in the other three groups ( P <0.05), the total VFA in the CON group was significantly lower than that in the other three groups ( P <0.05); In the ileum, the pH of the CON group was significantly lower than that of the other three groups ( P <0.05), the NH3-N content in the MIX group was significantly higher than that in the other three groups ( P <0.05), the acetic acid and total VFA content in group CON were significantly lower than those in the other three groups ( P <0.05).

[0040] The comprehensive experimental results show that vitamin E can increase the daily weight gain of lambs; the synergistic effect of yeast selenium and vitamin E can effectively improve the gastrointestinal fermentation pattern, thereby promoting the growth and development of lambs.

[0041] In summary, this invention provides an application of yeast selenium and vitamin E in synergistically improving the growth and gastrointestinal performance of mutton sheep. By adding yeast selenium and vitamin E to the basal diet of mutton sheep, the two work synergistically to effectively improve the growth capacity and gastrointestinal performance of mutton sheep, thereby comprehensively improving the quality of mutton sheep products.

[0042] The embodiments described above are some, but not all, embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. The synergistic application of yeast selenium and vitamin E in improving the growth and gastrointestinal function of meat sheep.

2. The application according to claim 1, characterized in that, By adding yeast selenium and vitamin E to the basic feed formula for mutton sheep, a total mixed pellet feed is prepared for feeding, thereby improving the growth capacity, rumen fermentation, and intestinal fermentation performance of mutton sheep; wherein, the amount of yeast selenium added is 0.6 mg / kg, and the amount of vitamin E added is 200 IU / kg, and the addition amount refers to the addition level per kilogram of basic feed for mutton sheep.

3. A total mixed pelleted feed for meat sheep, characterized in that, Its raw materials include yeast selenium, vitamin E, and a basal diet; wherein the amount of yeast selenium added is 0.6 mg / kg, and the amount of vitamin E added is 200 IU / kg, and the amount of addition refers to the level of addition per kilogram of basal diet.

4. The total mixed pelleted feed for meat sheep according to claim 3, characterized in that, The raw materials for the basic diet include corn, corn middlings, soybean meal, corn stalk pellets, sunflower seed shells, sprayed corn husks, dried corn lees, pumpkin seed shells, limestone powder, sodium bicarbonate, and premixed feed.

5. The total mixed pelleted feed for meat sheep according to claim 4, characterized in that, The stone powder in the basal diet has a particle size of 40 mesh.

6. The total mixed pelleted feed for meat sheep according to claim 4, characterized in that, The raw material components of the basal diet, by weight percentage, include: 32.04% corn, 6% corn middlings, 6% soybean meal, 14% corn stalk pellets, 12.2% sunflower seed shells, 8% sprayed corn husks, 5% dried corn lees, 10% pumpkin seed shells, 2.16% limestone powder, 0.6% sodium bicarbonate, and 4% premix.

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