Use of natural clinoptilolite in the preparation of feed for preventing postpartum paresis in organic dairy cows during the perinatal period

Abstract

The application discloses application of natural clinoptilolite in preparation of feed for preventing postpartum paralysis of organic farm perinatal dairy cows. In the application, only the natural clinoptilolite is used, and calcium and phosphorus metabolism of a matrix is regulated by preferential adsorption of phosphorus, so that the problem that postpartum paralysis of dairy cows cannot be well prevented in an organic farm for a long time is solved.

Description

Technical Field

[0001] This invention relates to the field of livestock breeding and feed additive technology. More specifically, it relates to the application of a natural clinoptilolite in the preparation of feed for preventing postpartum paralysis in dairy cows on organic farms. Background Technology

[0002] Postpartum paralysis (also known as puerperal fever) is one of the most common metabolic diseases in dairy cows during the peripartum period, mainly caused by a sharp drop in blood calcium concentration before and after calving. Postpartum paralysis not only leads to cows becoming unable to stand and being culled, but also significantly increases the risk of secondary diseases such as retained placenta, metritis, displaced abomasum, and mastitis, causing serious economic losses to farms. Current preventative techniques mainly include feeding low-calcium diets before calving and using anionic salts (such as ammonium chloride and magnesium sulfate). However, these methods have the following drawbacks: 1. Feed ingredients naturally contain high levels of calcium, making it difficult to formulate low-calcium diets that meet standards and to accurately control calcium content; 2. Anionic salts have poor palatability and are prone to causing metabolic acidosis; they are difficult to mix evenly during production, and individual feeding is uneven, requiring reliance on urine pH monitoring with a low compliance rate. 3. The above methods mostly involve chemically synthesized substances, which do not comply with the requirements of the permitted list of feed additives for organic ranches (GB / T 19630).

[0003] Existing studies have confirmed that the natural aluminosilicate mineral clinoptilolite possesses ion exchange and adsorption properties, and most current applications achieve health benefits by adsorbing calcium ions in the intestines. For example, CN117814376A describes the compounding of clinoptilolite with vitamins, magnesium oxide, zinc sulfate, and *Lysimachia christinae* extract, resulting in a complex formula and high production costs. Furthermore, magnesium oxide and zinc sulfate are artificially processed or chemically synthesized raw materials, which do not meet the requirements for use in organic feed as specified in GB / T19630. Currently, the industry generally relies on zeolite to adsorb dietary calcium and create a low-calcium diet environment to control hypocalcemia. However, organic farms have strict restrictions on the composition of feed additives (GB / T19630), and existing compound formulations cannot be used in organic farms due to their mineral salt content. Summary of the Invention

[0004] Based on the above problems, the purpose of this invention is to provide an application of natural clinoptilolite in the preparation of feed for preventing postpartum paralysis in dairy cows during the peripartum period on organic farms, and a feeding method for preventing postpartum paralysis in dairy cows during the peripartum period on organic farms. Organic farms have strict restrictions on the composition of feed additives (GB / T 19630), and existing compound formulations cannot be used on organic farms because they contain mineral salts. The technical solution of this invention provides for the first time an effective technical solution that fully complies with organic standards, using a single natural clinoptilolite, relying on preferential phosphorus adsorption to regulate the calcium and phosphorus metabolism of the matrix, thus solving the long-standing problem of difficulty in effectively preventing postpartum paralysis in dairy cows on organic farms.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides the application of natural clinoptilolite in the preparation of feed for preventing postpartum paralysis in dairy cows during the peripartum period on organic ranches.

[0006] Furthermore, the method of application is as follows: the natural clinoptilolite is added to the feed as a feed additive.

[0007] Furthermore, the feed is peripartum cattle TMR.

[0008] Furthermore, the natural clinoptilolite has a particle size of 80-200 mesh and a purity of ≥80%.

[0009] Furthermore, in the feed, the amount of natural clinoptilolite added is 1.5 to 3.5% of the total dry matter weight of the feed.

[0010] Furthermore, the amount of natural clinoptilolite added to the feed is 2.5% of the total dry matter weight of the feed.

[0011] Natural clinoptilolite, relying on its unique porous structure and surface silica-alumina hydroxyl functional groups, preferentially and selectively adsorbs dietary inorganic phosphorus under different pH conditions in the digestive tract of dairy cows. Its adsorption of calcium ions is weak, thus reducing intestinal phosphorus absorption, creating a physiological low-phosphorus signal, and regulating PTH-1,25(OH)2D3. The FGF23 endocrine axis mobilizes bone calcium reserves in advance, preventing a sudden drop in blood calcium levels during calving and lactation. This differs from the traditional approach of existing zeolites, which work by adsorbing calcium and artificially creating low-calcium diets. In this invention, natural clinoptilolite in the aforementioned amount is added to the feed as a single feed additive (without any added vitamins, mineral salts (such as magnesium oxide, zinc sulfate, magnesium sulfate), or plant extracts (such as *Lysimachia christinae*), fully complying with the permitted requirements for mineral feeds in the national standard GB / T 19630 for organic farms). This significantly improves the problem of postpartum paralysis in dairy cows during the peripartum period in organic farms, which is often difficult to prevent due to strict restrictions on feed additives.

[0012] Furthermore, the natural clinoptilolite is a single feed additive in the feed.

[0013] Furthermore, the application methods include: The feed was started 21±3 days before calving and continued until calving.

[0014] Secondly, the present invention provides a feeding method for preventing postpartum paralysis in dairy cows during the peripartum period of organic farms, comprising the following steps: Feed containing natural clinoptilolite is used to feed peripartum dairy cows on organic ranches.

[0015] Furthermore, the method includes the following steps: The dairy cows were fed the aforementioned feed from 21±3 days before their expected calving date until calving.

[0016] Furthermore, the amount of natural clinoptilolite added to the feed is 1.5 to 3.5% of the total dry matter weight of the feed.

[0017] Furthermore, the amount of natural clinoptilolite added to the feed is 2.5% of the total dry matter weight of the feed.

[0018] Furthermore, the natural clinoptilolite is a single feed additive in the feed.

[0019] Furthermore, the natural clinoptilolite has a particle size of 80-200 mesh and a purity of ≥80%.

[0020] The beneficial effects of this invention are as follows: The technical solution of this invention significantly reduces the incidence of postpartum paralysis in dairy cows during the peripartum period on organic farms: large-scale trials have confirmed that the incidence rate has decreased from 15% to 4%, a relative reduction of 73.3% (P<0.001); moreover, this technical solution complies with organic farm standards: it is a single natural mineral, contains no prohibited synthetic additives, and can pass organic certification; the application method of this invention is simple to operate and low in cost: it only requires mixing natural clinoptilolite into the prepartum diet, without the need to compound multiple expensive ingredients; it has good palatability: it does not affect dry matter intake; and it is environmentally friendly: zeolite can absorb ammonia in manure, reducing pollution in the farming environment. Detailed Implementation

[0021] To more clearly illustrate the present invention, the following description, in conjunction with preferred embodiments, further clarifies the invention. Those skilled in the art should understand that the specific descriptions below are illustrative rather than restrictive, and should not be construed as limiting the scope of protection of the present invention.

[0022] Example (Large-scale feeding trial on an organic ranch) Test location and subjects: The experiment was conducted at a large-scale organic ranch in Hulunbuir City, Inner Mongolia Autonomous Region, which has obtained China Organic Product Certification (GB / T 19630). Forty hundred Holstein dairy cows of similar parity (2-4 gestation), body condition scores (3.0-3.25 out of 5), and expected calving dates were randomly divided into a control group and an experimental group, with 200 cows in each group.

[0023] Experimental Design: Control group (200 animals): fed pasture-based total mixed organic diet (TMR) with a calcium content of 0.58 wt% and a phosphorus content of 0.34 wt% (dry matter basis), starting 21 days before farrowing and continuing until farrowing.

[0024] Experimental group (200 heads): In addition to the basal diet, natural clinoptilolite powder (origin: Inner Mongolia, purity 86%, identified as clinoptilolite type by X-ray diffraction, particle size 100 mesh) was added at an amount of 2.5w of the total dry matter of the diet, and fed until farrowing.

[0025] Except for supplements, the feeding and management conditions (cattle shed, water, lighting, milking, etc.) of the two groups of dairy cows were exactly the same.

[0026] Observation indicators From calving to 72 hours postpartum, a dedicated veterinarian observed and recorded daily whether the dairy cows developed postpartum paralysis (defined as: inability to stand or difficulty standing after calving, lying down and unable to get up, recovering after intravenous calcium supplementation). The number of cows with postpartum paralysis and the incidence rate were recorded in both groups.

[0027] The experimental results are shown in Table 1 below.

[0028] Table 1: Experimental Results

[0029] Statistical analysis: The chi-square test showed χ² = 13.89 (df = 1), P = 0.00019 ( P The difference in incidence rates between the two groups was statistically significant (<0.001).

[0030] Summary of Results: The incidence of postpartum paralysis in the experimental group was 4%, while that in the control group was 15%. The incidence rate in the experimental group was 11 percentage points lower than that in the control group, a relative decrease of 73.3% (i.e., a decrease of about two-thirds).

[0031] It is evident that this invention was validated under large-scale production conditions with 400 peripartum dairy cows (200 cows per group). Compared to the control, adding natural clinoptilolite (2.5 wt% dry matter basis, fed alone) to the prepartum diet of peripartum dairy cows on organic farms significantly reduced the incidence of postpartum paralysis (P<0.001), from 15% to 4%, a relative reduction of 73.3%, which is statistically significant. Existing literature is mostly small-scale exploratory studies (usually 20-50 cows per group). The large-scale validation of this invention significantly enhances the credibility and industrial application value of the technical solution. This technical solution is simple, effective, and compliant, suitable for large-scale promotion and application on organic farms.

[0032] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. All obvious variations or modifications derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

1. Application of natural clinoptilolite in the preparation of feed for preventing postpartum paralysis in dairy cows during the peripartum period on organic farms.

2. The application according to claim 1, characterized in that, The method of application is as follows: the natural clinoptilolite is added to the feed as a feed additive.

3. The application according to claim 1 or 2, characterized in that, The natural clinoptilolite has a particle size of 80-200 mesh and a purity of ≥80%.

4. The application according to claim 1 or 2, characterized in that, In the feed, the amount of natural clinoptilolite added is 1.5 to 3.5% of the total dry matter weight of the feed.

5. The application according to claim 1 or 2, characterized in that, In the feed, the amount of natural clinoptilolite added is 2.5% of the total dry matter weight of the feed.

6. The application according to claim 1 or 2, characterized in that, The natural clinoptilolite is the sole feed additive in the feed.

7. The application according to claim 1 or 2, characterized in that, The application methods include: The feed was started 21±3 days before calving and continued until calving.

8. A feeding method for preventing postpartum paralysis in dairy cows during the peripartum period on organic farms, characterized in that, Includes the following steps: Feed containing natural clinoptilolite is used to feed peripartum dairy cows on organic ranches.

9. The feeding method according to claim 8, characterized in that, Includes the following steps: The dairy cows were fed the aforementioned feed from 21±3 days before their expected calving date until calving.

10. The feeding method according to claim 8 or 9, characterized in that, In the feed, the amount of natural clinoptilolite added is 1.5 to 3.5% of the total dry matter weight of the feed.

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