Enzymatic and fermented pig feed additive and its application in diarrhea prevention and control

Abstract

The present application relates to a kind of enzymatic and fermented pig feed additive and its application in diarrhea prevention and control, belong to the technical field of feed additive.The present application provides a kind of pig feed additive, including the following mass fraction components, sophora 5~15 parts, Bo Luo Hui 5~15 parts, Coptis 5~11 parts and licorice 5~11 parts.Pig feed additive prepared by the method of the present application, each component has synergistic effect, enzymolysis in preparation method can release active ingredient added, fermentation can degrade macromolecular substances into small molecule form easy to absorb, can also impart product aroma, improve the palatability of feed, while improving the growth performance of weaned piglets can also reduce its diarrhea rate.

Description

Technical Field

[0001] This invention relates to the field of feed additive technology, and in particular to an enzymatically hydrolyzed and fermented pig feed additive and its application in diarrhea prevention and control. Background Technology

[0002] In pig farming, weaning stress and pathogen infection are the core contributing factors to diarrhea, growth retardation, weakened immunity, and even death in piglets. Meanwhile, with consumers' increasing demands for pork quality, effectively reducing subcutaneous fat deposition and increasing lean meat percentage has become a crucial research direction in pig breeding and nutritional regulation. Traditionally, the livestock industry has extensively used antibiotics as feed additives for disease prevention and growth promotion, but long-term overuse has led to increasingly serious problems of bacterial resistance and drug residues. my country has banned the addition of feed additives containing growth-promoting drugs to feed, necessitating the search for safe antibiotic alternatives that combine growth promotion, disease control, and meat quality improvement.

[0003] Traditional Chinese medicine and its extracts have broad prospects as natural antibiotic alternatives. Sophora japonica fruit has anti-inflammatory, antioxidant, hepatoprotective, choleretic, and astringent effects; *Polygonum multiflorum* and its main active ingredients, sanguisorbin and chelidonine, can reduce intestinal inflammation by inhibiting the NF-κB-mediated inflammatory pathway, while also possessing broad-spectrum antibacterial activity; *Coptis chinensis* and its main active ingredient, berberine, have broad-spectrum antibacterial, anti-inflammatory, and lipid metabolism-regulating effects; licorice has anti-inflammatory, antioxidant, immune-enhancing, and drug-harmonizing effects. However, existing technologies mostly employ direct pulverization and mixing or water- and alcohol-extraction processes, which suffer from low release rates of effective components, poor bioavailability, and poor palatability. The existing patent "Polygonum multiflorum and Coptis chinensis powder" (CN103623078A) directly pulverizes and mixes *Polygonum multiflorum*, *Coptis chinensis*, and licorice for treating livestock diarrhea, but it does not involve the Sophora japonica fruit component, nor does it employ a fermentation process. Currently, there is no systematic research or patent disclosure on the preparation of pig feed additives through the synergistic fermentation of four medicinal herbs: Sophora japonica fruit, Coptis chinensis, Coptis chinensis, and Glycyrrhiza uralensis.

[0004] Based on this, the present invention is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide an enzymatically hydrolyzed and fermented pig feed additive and its application in diarrhea prevention and control, so as to solve the problem that there is a lack of feed additives in the prior art that have the functions of growth promotion, disease prevention and control and meat quality improvement.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a feed additive for pigs, comprising the following components in parts by weight: 5-15 parts Sophora japonica fruit, 5-15 parts Clematis chinensis, 5-11 parts Coptis chinensis and 5-11 parts Glycyrrhiza uralensis.

[0007] This invention provides a method for preparing the aforementioned pig feed additive, comprising the following steps: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, and pulverize to obtain mixed medicinal powder; (2) Mix the mixed powder with the compound enzyme preparation, adjust the moisture content to 45-65%, enzymatically hydrolyze for 2-6 hours, and sterilize to obtain the hydrolysate; (3) Mix the enzymatic hydrolysate with the compound bacterial liquid and ferment for 48-72 hours to obtain the fermentation product. Dry and pulverize to obtain a pig feed additive.

[0008] Preferably, the particle size of the pulverized material in step (1) is 40-60 mesh.

[0009] Preferably, in step (2), the mass ratio of the mixed drug powder to the compound enzyme preparation is 100:0.1~1; The compound enzyme preparation includes cellulase, xylanase, pectinase and β-glucosidase; The mass ratio of cellulase, xylanase, pectinase and β-glucosidase is 2~4:1~3:1~2:1~2.

[0010] Preferably, the pH value of the enzymatic hydrolysis in step (2) is 5.0~7.0 and the temperature is 45~55℃.

[0011] Preferably, the mass-to-volume ratio of the enzymatic hydrolysate to the compound bacterial solution in step (3) is 100g: 5~15mL; The viable count of the compound bacterial solution is not less than 1×10⁻⁶. 8 CFU / mL; The compound bacterial solution includes Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae, with a live cell ratio of 1~3:1~3:1~2.

[0012] Preferably, the fermentation in step (3) is anaerobic fermentation, with a fermentation temperature of 30~37℃, a humidity of 75%~85%, and a pH value of 5.5~6.5.

[0013] Preferably, the particle size of the pulverized material in step (3) is 60~120 mesh.

[0014] This invention provides the application of the pig feed additive described above or the pig feed additive prepared by the described method in the prevention and control of swine diarrhea.

[0015] This invention provides a method for preventing and controlling diarrhea in pigs, which involves mixing a basic diet with a pig feed additive to obtain a mixed feed, which is then used to feed pigs. The mass ratio of the basal diet to the pig feed additive is 100:0.1~0.5; The pig feed additive is the pig feed additive described above or the pig feed additive prepared by the preparation method described above.

[0016] The present invention has the following technical effects and advantages: This invention relates to a pig feed additive that combines Sophora japonica fruit with Angelica sinensis, Coptis chinensis, and Glycyrrhiza uralensis. The components work synergistically, further enhancing the effect of regulating the gastrointestinal tract and reducing diarrhea compared to a combination of Angelica sinensis, Coptis chinensis, and Glycyrrhiza uralensis. In terms of preparation, this invention utilizes a compound enzyme preparation to lyse plant cell walls, fully releasing intracellular active ingredients. Simultaneously, fermentation with a compound bacterial solution further promotes metabolic transformation, degrading macromolecules into smaller, easily absorbed forms. The fermentation also produces lactic acid, acetic acid, and other organic acids and aromatic alcohols and esters, effectively masking the strong bitterness of Coptis chinensis and other medicinal herbs, giving the product a sour and fragrant aroma and improving palatability. In pig farming, the pig feed additive prepared using this method significantly improves the growth performance of pigs while reducing their diarrhea rate. Detailed Implementation

[0017] This invention provides a feed additive for pigs, comprising the following components in parts by weight: Sophora japonica fruit 5-15 parts, Clematis chinensis 5-15 parts, Coptis chinensis 5-11 parts and Glycyrrhiza uralensis 5-11 parts; The preferred mass fraction of the locust fruit is 8 to 12 parts, more preferably 10 parts; The preferred mass fraction of the boluohui is 8 to 12 parts, more preferably 10 parts; The preferred mass fraction of Coptis chinensis is 7 to 9 parts, more preferably 8 parts; The preferred mass fraction of the licorice is 7 to 9 parts, more preferably 8 parts.

[0018] This invention provides a method for preparing the aforementioned pig feed additive, comprising the following steps: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, and pulverize to obtain mixed medicinal powder; (2) Mix the mixed powder with the compound enzyme preparation, adjust the moisture content to 45-65%, enzymatically hydrolyze for 2-6 hours, and sterilize to obtain the hydrolysate; The adjusted moisture content is preferably 55%; the enzymatic hydrolysis time is preferably 4 hours. The sterilization method is to heat the product to 95°C, keep it at that temperature for 45 minutes, and then cool it to 35°C. (3) Mix the enzymatic hydrolysate with the compound bacterial liquid and ferment for 48-72 hours to obtain the fermentation product. Dry and pulverize the product to obtain a pig feed additive. The fermentation time is preferably 60 hours; The drying method involves drying the fermentation product at a temperature of 55°C until the moisture content is less than or equal to 10%.

[0019] In this invention, the particle size of the pulverized material in step (1) is 40-60 mesh, preferably 50 mesh.

[0020] In this invention, the mass ratio of the mixed powder to the compound enzyme preparation in step (2) is 100:0.1~1, preferably 100:0.5; The compound enzyme preparation includes cellulase, xylanase, pectinase and β-glucosidase; The mass ratio of cellulase, xylanase, pectinase and β-glucosidase is 2~4:1~3:1~2:1~2, preferably 3:2:1:1.

[0021] In this invention, the pH value of the enzymatic hydrolysis in step (2) is 5.0~7.0, and the temperature is 45~55℃; The pH value is preferably 6.0, and the temperature is preferably 50°C.

[0022] In this invention, the mass-to-volume ratio of the enzymatic hydrolysate to the compound bacterial solution in step (3) is 100g: 5~15mL, preferably 100g: 10mL; The viable count of the compound bacterial solution is not less than 1×10⁻⁶. 8 CFU / mL; The compound bacterial solution includes Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae, with the live cell ratio of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae being 1~3:1~3:1~2, preferably 2:2:1.

[0023] In this invention, the fermentation in step (3) is anaerobic fermentation, with a fermentation temperature of 30~37℃, a humidity of 75%~85%, and a pH value of 5.5~6.5; The preferred temperature is 34°C, the preferred humidity is 80%, and the preferred pH value is 6.0.

[0024] In this invention, the particle size of the pulverized material in step (3) is 60-120 mesh, preferably 80-100 mesh, and more preferably 90 mesh.

[0025] This invention provides the application of the pig feed additive described above or the pig feed additive prepared by the described method in the prevention and control of swine diarrhea.

[0026] This invention provides a method for preventing and controlling diarrhea in pigs, which involves mixing a basic diet with a pig feed additive to obtain a mixed feed, which is then used to feed pigs. The mass ratio of the basal diet to the pig feed additive is 100:0.1~0.5, preferably 100:0.3; The pig feed additive is the pig feed additive described above or the pig feed additive prepared by the preparation method described above.

[0027] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0028] Example 1

[0029] A pig feed additive consists of 10 kg of Sophora japonica fruit, 10 kg of Angelica sinensis, 8 kg of Coptis chinensis and 8 kg of Glycyrrhiza uralensis.

[0030] Preparation of compound enzyme preparation: Mix cellulase, xylanase, pectinase and β-glucosidase in a mass ratio of 3:2:1:1.

[0031] Preparation of compound bacterial culture: Activated Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained by activating the bacterial strains. The Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were then cultured on a large scale, and the viable cell count concentration of each was adjusted to 2 × 10⁻⁶. 8 CFU / mL, Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained separately. These three cultures were then mixed in a volume ratio of 2:2:1 to obtain a total viable count of 2 × 10⁻⁶. 8 A compound bacterial solution with CFU / mL containing Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae in a live cell ratio of 2:2:1.

[0032] The preparation method of pig feed additives is as follows: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, pulverize and pass through a 50-mesh sieve to obtain mixed medicinal powder; (2) Mix the mixed powder and the compound enzyme preparation in a mass ratio of 100:0.5, add water to adjust the water content to 50%, adjust the pH to 6.0 with citric acid, and enzymatically hydrolyze at 50°C for 4 hours, stirring twice during the process. Then, heat the product to 95°C, keep it at the temperature for 45 minutes to sterilize, and then cool it to 35°C to obtain the enzymatic hydrolysate. (3) Mix the enzymatic hydrolysate and the compound bacterial solution at a mass-volume ratio of 100g:10mL, put them into a stainless steel fermentation tank, control the temperature at 34℃, humidity at 80%, and pH at 6.0, and carry out anaerobic fermentation. Turn the feed once every 12 hours during the fermentation. After 60 hours of fermentation, dry the fermentation product to a moisture content of 8% at a temperature of 55℃, and crush it through a 90-mesh sieve to obtain a pig feed additive.

[0033] Example 2

[0034] A pig feed additive consists of 8 kg of Sophora japonica fruit, 12 kg of Angelica sinensis, 7 kg of Coptis chinensis and 9 kg of Glycyrrhiza uralensis.

[0035] Preparation of compound enzyme preparation: Mix cellulase, xylanase, pectinase and β-glucosidase in a mass ratio of 2:3:1:1.

[0036] Preparation of compound bacterial culture: Activated Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained. These were then cultured on a large scale, and the viable cell count concentration of each culture was adjusted to 2 × 10⁻⁶. 8 CFU / mL of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained, respectively. These three cultures were then mixed in a volume ratio of 3:3:1 to obtain a total viable count of 2 × 10⁻⁶. 8 A compound bacterial solution with CFU / mL containing Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae in a live cell ratio of 3:3:1.

[0037] The preparation method of pig feed additives is as follows: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, pulverize and pass through a 60-mesh sieve to obtain mixed medicinal powder; (2) Mix the mixed powder and the compound enzyme preparation at a mass ratio of 100:1, add water to adjust the water content to 48%, adjust the pH to 6.0 with citric acid, and enzymatically hydrolyze at 50°C for 5 hours, stirring twice during the process. Then, heat the product to 95°C, keep it at the temperature for 45 minutes to sterilize, and then cool it to 35°C to obtain the enzymatic hydrolysate. (3) Mix the enzymatic hydrolysate and the compound bacterial solution at a mass-volume ratio of 100g:12mL, put them into a stainless steel fermentation tank, control the temperature at 34℃, humidity at 80%, and pH at 6.0, and carry out anaerobic fermentation. Turn the feed once every 12 hours during the fermentation. After 60 hours of fermentation, dry the fermentation product to a moisture content of 6% at a temperature of 55℃, and crush it through a 100-mesh sieve to obtain a pig feed additive.

[0038] Example 3

[0039] A pig feed additive consists of 12 kg of Sophora japonica fruit, 8 kg of Angelica sinensis, 9 kg of Coptis chinensis and 9 kg of Glycyrrhiza uralensis.

[0040] Preparation of compound enzyme preparation: Mix cellulase, xylanase, pectinase and β-glucosidase in a mass ratio of 3:3:1:1.

[0041] Preparation of compound bacterial culture: Activated Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained. These were then cultured on a large scale, and the viable cell count concentration of each culture was adjusted to 2 × 10⁻⁶. 8 CFU / mL of Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae were obtained, respectively. These three cultures were then mixed at a volume ratio of 3:2:1 to obtain a total viable count of 2 × 10⁻⁶. 8 A compound bacterial solution with CFU / mL containing Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae in a live cell ratio of 3:2:1.

[0042] The preparation method of pig feed additives is as follows: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, pulverize and pass through a 45-mesh sieve to obtain mixed medicinal powder; (2) Mix the mixed powder and the compound enzyme preparation in a mass ratio of 100:0.4, add water to adjust the water content to 48%, adjust the pH to 6.0 with citric acid, and enzymatically hydrolyze at 50°C for 3 hours, stirring twice during the process. Then, heat the product to 95°C, keep it at the temperature for 45 minutes to sterilize, and then cool it to 35°C to obtain the enzymatic hydrolysate. (3) Mix the enzymatic hydrolysate and the compound bacterial solution at a mass-volume ratio of 100g:8mL, put them into a stainless steel fermentation tank, control the temperature at 34℃, humidity at 80%, and pH at 6.0, and carry out anaerobic fermentation. Turn the feed once every 12 hours during the fermentation. After 60 hours of fermentation, dry the fermentation product to a moisture content of 7% at a temperature of 55℃, and crush it through an 80-mesh sieve to obtain a pig feed additive.

[0043] Comparative Example 1

[0044] This pig feed additive consists of 10 kg of Angelica sinensis, 5 kg of Coptis chinensis, and 5 kg of Glycyrrhiza uralensis.

[0045] The preparation method is as follows: Weigh out Bo Luo Hui (Radix Aristolochiae), Huang Lian (Radix Coptise), and Gan Cao (Radix Glycyrrhizae), pulverize and dry them separately, and pass them through a 100-mesh sieve to obtain Bo Luo Hui powder, Huang Lian powder, and Gan Cao powder respectively. Add Bo Luo Hui powder, Huang Lian powder, and Gan Cao powder to a mixer in equal increments and mix for 15 minutes to obtain a pig feed additive.

[0046] Comparative Example 2

[0047] Compared with Example 1, Astragalus membranaceus was used instead of Sophora japonica fruit, the amount of which remained the same, the amount of other components was the same, and the preparation method was the same as in Example 1.

[0048] Comparative Example 3

[0049] Compared with Example 1, only the preparation method is different. The preparation method is as follows: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, pulverize and pass through a 50-mesh sieve to obtain mixed medicinal powder; (2) Mix the mixed medicinal powder and the compound bacterial liquid at a mass-volume ratio of 100g:10mL, put them into a stainless steel fermentation tank, control the temperature at 34℃, humidity at 80%, pH at 6.0, and carry out anaerobic fermentation. During the fermentation, turn the feed once every 12 hours. After 60 hours of fermentation, dry the fermentation product to a moisture content of 8% at a temperature of 55℃, and crush it through a 90-mesh sieve to obtain a pig feed additive.

[0050] Test case

[0051] Three hundred piglets weighing 9±0.5 kg after weaning and nursery stage were selected and randomly divided into 5 groups (control group, treatment groups 1-3), 20 piglets per group, with each treatment repeated 3 times. They were kept in isolation and each group was immunized and fed according to the routine immunization program.

[0052] The control group was fed only a basal diet, sourced from Binzhou Huijia Biotechnology Co., Ltd. Treatment group 1 received 0.5% (by weight) of Example 1 pig feed additive mixed into its basal diet; treatment group 2 received 0.5% (by weight) of Comparative Example 1 pig feed additive mixed into its basal diet; treatment group 3 received 0.5% (by weight) of Comparative Example 2 pig feed additive mixed into its basal diet; and treatment group 4 received 0.5% (by weight) of Comparative Example 3 pig feed additive mixed into its basal diet. Feeding was conducted twice daily, at 8:00 AM and 4:00 PM, and the amount fed was recorded. Piglets were allowed free access to feed and water for 30 days. At the beginning and end of the experiment, the weight of weaned piglets in each group was measured. Daily feed intake was recorded for each group during the experiment, and the number of weaned piglets with diarrhea was also counted.

[0053] After the experiment, the average daily weight gain (g / d), average daily feed intake (g / d), feed conversion ratio and diarrhea rate (%) of weaned piglets in each treatment group were calculated. The average value was taken from three replicates to compare the growth of weaned piglets in different treatment groups. The results are shown in Table 1.

[0054] Average daily weight gain = (final weight - initial weight) / number of days in the trial; The average daily feed intake is calculated by dividing the total daily feed intake of weaned piglets during the experiment by the number of days in the experiment. Feed conversion ratio = average daily feed intake / average daily weight gain; Diarrhea rate = (number of piglets with obvious diarrhea / total number of weaned piglets) × 100%.

[0055] Table 1. Growth of weaned piglets in different treatment groups

[0056] As shown in Table 1, the average daily weight gain and average daily feed intake of weaned piglets in treatment groups 1-4 were higher than those in the control group, with treatment group 1 showing the highest average daily weight gain and average daily feed intake. This indicates that adding the pig feed additives of Example 1 and Comparative Examples 1-3 can improve the daily weight gain and feed intake of piglets, with the pig feed additive of Example 1 showing the best effect. The feed conversion ratio and diarrhea rate of weaned piglets in treatment groups 1-4 were significantly lower than those in the control group, with treatment group 1 showing the lowest feed conversion ratio and diarrhea rate. This indicates that adding the pig feed additives of Example 1 and Comparative Examples 1-3 can improve the growth performance of weaned piglets and effectively reduce the morbidity rate in piglets, with the pig feed additive of Example 1 showing the best effect.

[0057] As can be seen from the above embodiments, the present invention provides an enzymatically hydrolyzed and fermented pig feed additive and its application in diarrhea prevention and control. The pig feed additive prepared by the method of the present invention has synergistic effects among its components. Enzymatic hydrolysis in the preparation method releases the added active ingredients, and fermentation degrades macromolecules into smaller, easily absorbed molecules, while also imparting aroma and flavor to the product, improving palatability. This not only improves the growth performance of weaned piglets but also reduces their diarrhea rate.

[0058] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A pig feed additive, characterized in that, The components include the following parts by mass: 5-15 parts Sophora japonica fruit, 5-15 parts Clematis chinensis, 5-11 parts Coptis chinensis and 5-11 parts Glycyrrhiza uralensis.

2. The method for preparing the pig feed additive according to claim 1, characterized in that, Includes the following steps: (1) Mix Sophora japonica fruit, Bo Luo Hui, Coptis chinensis and licorice, and pulverize to obtain mixed medicinal powder; (2) Mix the mixed powder with the compound enzyme preparation, adjust the moisture content to 45-65%, enzymatically hydrolyze for 2-6 hours, and sterilize to obtain the hydrolysate; (3) Mix the enzymatic hydrolysate with the compound bacterial liquid and ferment for 48-72 hours to obtain the fermentation product. Dry and pulverize to obtain a pig feed additive.

3. The preparation method according to claim 2, characterized in that, The particle size of the pulverized material in step (1) is 40~60 mesh.

4. The preparation method according to claim 2, characterized in that, In step (2), the mass ratio of the mixed powder to the compound enzyme preparation is 100:0.1~1; The compound enzyme preparation includes cellulase, xylanase, pectinase and β-glucosidase; The mass ratio of cellulase, xylanase, pectinase and β-glucosidase is 2~4:1~3:1~2:1~2.

5. The preparation method according to claim 2, characterized in that, The pH value of the enzymatic hydrolysis in step (2) is 5.0~7.0 and the temperature is 45~55℃.

6. The preparation method according to claim 2, characterized in that, The mass-to-volume ratio of the enzymatic hydrolysate to the compound bacterial solution in step (3) is 100g: 5~15mL; The viable count of the compound bacterial solution is not less than 1×10⁻⁶. 8 CFU / mL; The compound bacterial solution includes Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae, with a live cell ratio of 1~3:1~3:1~2.

7. The preparation method according to claim 2, characterized in that, The fermentation described in step (3) is anaerobic fermentation, with a fermentation temperature of 30~37℃, a humidity of 75%~85%, and a pH value of 5.5~6.

5.

8. The preparation method according to claim 2, characterized in that, The particle size of the pulverized material in step (3) is 60~120 mesh.

9. The application of the pig feed additive according to claim 1 or the pig feed additive prepared by the preparation method according to claims 2 to 8 in the prevention and control of swine diarrhea.

10. A method for preventing and controlling diarrhea in pigs, characterized in that, Mix the basic diet with pig feed additives to obtain mixed feed, and feed the pigs with the mixed feed. The mass ratio of the basal diet to the pig feed additive is 100:0.1~0.5; The pig feed additive is the pig feed additive according to claim 1 or the pig feed additive prepared by the preparation method according to claims 2 to 8.

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