A fresh food cat food for simultaneously improving oral and intestinal health of cats and a method of preparing the same

Abstract

The application discloses a fresh food cat food for simultaneously improving cat oral cavity and intestinal tract health and a preparation method thereof, and belongs to the field of pet food. The fresh food cat food comprises fresh meat, animal offal, enzymatic meat, animal fat, functional plant raw materials, vitamin premix, mineral premix, taurine, prebiotics and functional additives, wherein the functional additives are a mixture of yucca extract and tea polyphenol. The application also provides a preparation method of the fresh food cat food, which comprises raw material pretreatment, enzymatic treatment, mixing and beating, extrusion molding, segmented low-temperature slow steaming curing and cooling packaging. Through animal experiment verification, the fresh food cat food can reduce fecal ammonia nitrogen by about 35%, significantly relieve fecal odor, directionally enrich beneficial bacteria groups such as Firmicutes, Blautia, Lachnospiraceae and other short-chain fatty acid-producing bacteria, optimize intestinal microecology, and has good palatability without significant difference in oral cavity health index compared with a control group.

Description

Technical Field

[0001] This invention belongs to the field of pet food technology, and in particular relates to a fresh cat food that simultaneously improves the oral and intestinal health of cats and its preparation method. Background Technology

[0002] With the increasing number of pet owners in cities, pet health issues are receiving growing attention. As strict carnivores, cats' gut health directly impacts nutrient digestion and absorption, immune function, and overall health. The gut microbiota plays a crucial role in regulating animal immune function and nutrient metabolism, and maintaining gut homeostasis is essential for host health.

[0003] Traditional extruded cat food is typically processed at temperatures above 120℃. This high-temperature processing leads to excessive protein denaturation and significant loss of heat-sensitive vitamins and flavor compounds. Furthermore, the raw materials often contain large amounts of grains such as wheat and corn, increasing the burden on the cat's digestive system and posing an allergy risk. In addition, the protein ingredients in traditional cat food are added directly without sufficient enzymatic pretreatment, resulting in low digestibility and absorption. Undigested protein enters the colon and undergoes putrefaction and fermentation, producing large amounts of harmful metabolites such as ammonia nitrogen, leading to severe fecal odor and negatively impacting the living environment.

[0004] Several attempts have been made in the prior art to improve the gut health of cats. For example, Chinese patent application CN118716523A discloses a cat food with enhanced gut health and its preparation method, whose formula includes 55-65% fresh chicken and 15-25% enzymatically hydrolyzed fresh chicken. Although this method uses enzymatically hydrolyzed chicken, its preparation process still employs traditional high-temperature processing methods and does not involve the systematic regulation of fecal ammonia nitrogen emissions and the structure of specific gut microbiota. Other patents improve the gut environment by adding probiotics or chemical deodorizers; however, probiotics are easily deactivated during processing and storage, and chemical deodorizers may affect the palatability of the cat food.

[0005] The oral cavity, as the beginning of the digestive tract, is closely related to gut health. However, there are currently no fresh cat food products that can simultaneously improve gut health and ensure oral safety. Existing methods for improving cat oral health (such as brushing, dental powder, and mouthwash) are cumbersome to implement, difficult to maintain long-term, and lack synergy with gut health improvements.

[0006] Therefore, a fresh cat food that simultaneously improves oral and intestinal health in cats and its preparation method were developed. Summary of the Invention

[0007] In order to overcome the above-mentioned defects of the prior art, the present invention provides a fresh cat food that simultaneously improves the oral and intestinal health of cats and its preparation method, which solves the problems of protein denaturation and nutrient loss caused by high-temperature processing of traditional cat food, low protein digestibility leading to increased fecal ammonia nitrogen, fecal odor and impact on intestinal health.

[0008] To achieve the above objectives, the present invention provides the following technical solution: A fresh cat food that simultaneously improves oral and intestinal health in cats and its preparation method comprises the following raw materials in weight percentages: 55%–65% fresh meat, 8%–15% animal offal, 12%–18% enzymatically hydrolyzed meat, 3%–6% animal fat, 2%–5% functional plant ingredients, 0.5%–1.5% vitamin premix, 0.5%–1.5% mineral premix, 0.2%–0.4% taurine, 0.5%–1.2% prebiotics, and 0.3%–0.8% functional additives; wherein the functional additives are a mixture of yucca extract and tea polyphenols.

[0009] Preferably, the fresh meat is at least one of chicken, duck, fish, rabbit, and pigeon; and the animal offal is at least one of chicken liver, chicken heart, and duck liver.

[0010] Preferably, the enzymatically hydrolyzed meat is a product obtained by enzymatically hydrolyzing fresh meat with protease at 45℃~55℃ for 2 to 6 hours.

[0011] Preferably, the functional plant material is at least one of yam, pumpkin, and sweet potato; the prebiotic is at least one of fructooligosaccharide, galactooligosaccharide, and inulin.

[0012] Preferably, the mass ratio of the yucca extract to tea polyphenols is 1:1 to 2:1.

[0013] Preferably, a method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats includes the following steps: S1. Clean the fresh meat and animal offal, remove the fascia and foreign objects, and cut into pieces; S2. Mix some fresh meat with protease and enzymatically hydrolyze at 45℃~55℃ for 2 to 6 hours to obtain enzymatically hydrolyzed meat paste; S3. Mix the pretreated fresh meat, animal offal, enzymatically hydrolyzed meat paste, animal fat, functional plant ingredients, vitamin premix, mineral premix, taurine, prebiotics and functional additives according to the stated mass percentages, and then grind them into a meat paste. S4. The minced meat is extruded into granules, the diameter of which is 6mm to 12mm; S5. The shaped granules are cooked in stages. The first stage is cooked at 80℃~85℃ for 12 minutes~15 minutes, and the second stage is cooked at 92℃~98℃ for 6 minutes~8 minutes. S6. Cool the cured granules to room temperature, vacuum pack or nitrogen-filled pack, and store at 0℃~4℃.

[0014] Preferably, the protease in step S2 is papain or trypsin.

[0015] Preferably, the diameter of the particles in step S4 is 8 mm to 10 mm.

[0016] Preferably, the total time for segmented steaming in step S5 does not exceed 25 minutes.

[0017] Preferably, the refrigeration temperature in step S6 is 0℃~4℃.

[0018] The technical effects and advantages of this invention, which describes a fresh cat food that simultaneously improves oral and intestinal health in cats and its preparation method, are as follows: 1. This invention significantly reduces fecal ammonia nitrogen: Animal experiments have verified that the fresh cat food of this invention can reduce the fecal ammonia nitrogen content to 59.54±11.53mg / g, which is about 35% lower than the control group (91.93±19.84mg / g) (P<0.001), effectively alleviating the problem of cat fecal odor from the source and improving the breeding environment.

[0019] 2. This invention specifically optimizes the intestinal microecology: The fresh cat food of this invention can significantly enrich beneficial bacteria such as Firmicutes, Broutidae, Trichophyceae, Trichophyles, Macrococcus, and Veillonaceae. Among them, Broutidae is an important short-chain fatty acid producer, which can protect the body's intestinal health and enhance the intestinal barrier function.

[0020] 3. The invention has good palatability and does not affect normal feeding: There were no significant differences in daily food intake, water consumption and final weight of cats in the experimental group compared with the control group (P>0.05), indicating that the fresh cat food of the present invention has good palatability.

[0021] 4. The invention is safe for oral health: Tests showed that there were no statistically significant differences in the scores of gums, plaque, tartar, and breath between the experimental group and the control group (P>0.05), indicating that the product of this invention will not have any adverse effects on the oral health of cats while improving intestinal health.

[0022] 5. This invention retains more nutrients: The invention adopts a low-temperature segmented slow steaming process (total steaming time not exceeding 25 minutes), which, compared with the traditional high-temperature extrusion process above 120℃, retains the activity of small peptides produced by enzymatic hydrolysis and the natural flavor and heat-sensitive nutrients in the raw materials to the greatest extent, thereby improving the digestibility and absorption rate of cat food. Attached Figure Description

[0023] Figure 1 This is a flowchart of a fresh cat food that simultaneously improves the oral and intestinal health of cats, as proposed in this invention, and its preparation method. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0025] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include," "contain," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "includes..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0026] refer to Figure 1This invention provides a fresh cat food that improves intestinal health and alleviates fecal odor, along with its preparation method. Addressing the technical problems of traditional cat food, such as excessive protein denaturation and loss of heat-sensitive nutrients due to high-temperature processing, and low protein digestibility leading to increased fecal ammonia nitrogen, fecal odor, and negative impacts on intestinal health, this invention provides a fresh cat food comprising 55%–65% fresh meat, 8%–15% animal offal, 12%–18% enzymatically hydrolyzed meat, 3%–6% animal fat, 2%–5% functional plant ingredients, 0.5%–1.5% vitamin premix, 0.5%–1.5% mineral premix, 0.2%–0.4% taurine, 0.5%–1.2% prebiotics, and 0.3%–0.8% functional additives. The functional additives are a mixture of yucca extract and tea polyphenols. The raw materials are pulped and shaped, then cooked using a two-stage low-temperature slow steaming process at 80℃–85℃ and 92℃–98℃. This invention significantly improves the digestibility and utilization of protein by combining a specific ratio of fresh meat, offal, and enzymatically hydrolyzed meat with a low-temperature segmented cooking process, thereby reducing fecal ammonia nitrogen by about 35%. At the same time, it selectively enriches beneficial bacteria such as Firmicutes, Broutella, and Trichophyton to produce short-chain fatty acids, thus improving intestinal health without adversely affecting cat oral health indicators.

[0027] Example 1 A fresh cat food that improves intestinal health and alleviates fecal odor is composed of the following ingredients by weight percentage: 50% fresh chicken, 10% fresh duck, 8% chicken liver, 4% chicken heart, 13% enzymatically hydrolyzed chicken, 5% chicken fat, 3% yam, 2% pumpkin, 1% vitamin premix, 1% mineral premix, 0.3% taurine, 0.7% fructooligosaccharides, and 0.5% functional additives (yucca extract and tea polyphenols mixed in a 1:1 weight ratio).

[0028] In this embodiment, the method for preparing enzymatically hydrolyzed chicken is as follows: fresh chicken is mixed with papain and enzymatically hydrolyzed at 50°C for 4 hours to obtain enzymatically hydrolyzed chicken paste.

[0029] The preparation method is as follows: S1. Clean the fresh chicken, fresh duck, chicken liver and chicken heart, remove the tendons and foreign objects, cut into small pieces and set aside; S2. Mix some fresh chicken meat with papain and hydrolyze it at 50°C for 4 hours to obtain enzymatically hydrolyzed chicken paste; S3. Mix the pre-treated fresh chicken, fresh duck, chicken liver, chicken heart, enzymatically hydrolyzed chicken paste, chicken oil, yam, pumpkin, vitamin premix, mineral premix, taurine, fructooligosaccharides and functional additives according to the specified ratio, and put them into a grinder to grind into a uniform meat paste. S4. Put the minced meat into the forming machine and extrude it into granules with a diameter of 8mm to 10mm; S5. Place the shaped pellets in a cooking device for segmented cooking and maturation: the first stage is cooking at 82℃ for 15 minutes, and the second stage is cooking at 95℃ for 8 minutes (total cooking time 23 minutes). S6. Cool the cured granules to room temperature, vacuum pack them, and store them at 4°C.

[0030] Example 2 A fresh cat food that improves intestinal health and alleviates fecal odor is composed of the following ingredients by weight percentage: 55% fresh fish (salmon and cod mixed in a 1:1 ratio), 5% chicken heart, 5% duck liver, 15% enzymatically hydrolyzed fish, 4% chicken fat, 4% pumpkin, 2% sweet potato, 1.2% vitamin premix, 0.8% mineral premix, 0.3% taurine, 0.5% galactooligosaccharides, 0.5% inulin, and 0.5% functional additives (yucca extract and tea polyphenols mixed in a 2:1 weight ratio).

[0031] In this embodiment, the method for preparing enzymatically hydrolyzed fish meat is as follows: fresh fish meat is mixed with trypsin and enzymatically hydrolyzed at 48°C for 3 hours to obtain enzymatically hydrolyzed fish meat paste.

[0032] The preparation method is the same as in Example 1, except that in step S5, the first stage is steamed at 80°C for 15 minutes, and the second stage is steamed at 92°C for 8 minutes (total steaming time is 23 minutes).

[0033] Example 3 A fresh cat food that improves intestinal health and alleviates fecal odor is composed of the following ingredients by weight percentage: 40% fresh rabbit meat, 20% fresh duck meat, 6% chicken liver, 4% chicken heart, 14% enzymatically hydrolyzed rabbit meat, 3% chicken fat, 4% yam, 3% pumpkin, 1% vitamin premix, 1% mineral premix, 0.25% taurine, 1% fructooligosaccharides, and 0.5% functional additives (yucca extract and tea polyphenols mixed at a weight ratio of 1.5:1).

[0034] In this embodiment, the preparation method of enzymatically hydrolyzed rabbit meat is as follows: fresh rabbit meat is mixed with a complex protease and enzymatically hydrolyzed at 52°C for 5 hours to obtain enzymatically hydrolyzed rabbit meat paste.

[0035] The preparation method is the same as in Example 1, except that in step S5, the first stage is steamed at 85°C for 12 minutes, and the second stage is steamed at 98°C for 6 minutes (total steaming time is 18 minutes).

[0036] Example 4 A fresh cat food that improves intestinal health and alleviates fecal odor is composed of the following ingredients by weight percentage: 60% fresh chicken meat, 8% chicken liver, 4% chicken heart, 12% enzymatically hydrolyzed chicken meat, 4% chicken fat, 3% yam, 2% pumpkin, 1% sweet potato, 1.2% vitamin premix, 1% mineral premix, 0.3% taurine, 0.8% fructooligosaccharides, 0.4% inulin, and 0.6% functional additives (yucca extract and tea polyphenols mixed at a weight ratio of 1.5:1).

[0037] The preparation method is the same as in Example 1.

[0038] Example 5 A fresh cat food that improves intestinal health and alleviates fecal odor is composed of the following ingredients by weight percentage: 45% fresh duck meat, 15% fresh fish meat, 6% duck liver, 5% chicken heart, 14% enzymatically hydrolyzed duck meat, 4% duck fat, 3% pumpkin, 2% sweet potato, 1% vitamin premix, 0.8% mineral premix, 0.3% taurine, 0.8% galactooligosaccharides, 0.4% inulin, and 0.5% functional additives (yucca extract and tea polyphenols mixed in a 1:1 weight ratio).

[0039] The preparation method is the same as in Example 1, except that in step S2, trypsin is used to enzymatically hydrolyze the enzyme at 50°C for 4 hours.

[0040] Comparative Example 1 A cat food is composed of the following ingredients by weight percentage: 55% fresh chicken meat, 10% chicken liver, 5% chicken heart, 6% chicken fat, 3% yam, 2% pumpkin, 1.2% vitamin premix, 1% mineral premix, 0.3% taurine, 0.8% fructooligosaccharides, 0.5% inulin, and 0.5% functional additives (yucca extract and tea polyphenols mixed in a 1:1 weight ratio). The difference from Example 1 is that enzymatically hydrolyzed meat is not added, and the amount of fresh chicken meat is increased accordingly.

[0041] The preparation method is the same as in Example 1.

[0042] Comparative Example 2 A cat food with the same raw material composition as in Example 1, except that step S5 in the preparation method adopts a traditional high-temperature cooking process: cooking at 121°C for 20 minutes.

[0043] Experimental Example 1 Experimental Design: The experiment was conducted at a pet breeding experimental facility from July to August 2025. Twenty healthy Linqing Lion Cats, weighing 2.93±0.34 kg and randomly assigned to either sex, were selected and divided into two groups of 10 cats each. The experimental group was fed the fresh cat food prepared according to Example 1 of this invention (50g daily) supplemented with basal food (50g daily), while the control group was fed basal food (80g daily). Each cat was housed individually in a pet cage (1.6m×0.7m×0.7m). The experiment lasted 28 days. Necessary immunizations and deworming treatments were performed before the experiment. The litter box was changed daily, and the enclosures were cleaned to maintain cleanliness.

[0044] During the trial period, cats were fed at 8:30 AM daily. The experimental group was fed 50g of fresh cat food from Example 1, supplemented with 50g of basal cat food, while the control group was fed 80g of basal food and 300mL of water. During the trial period, the feeding status and mental state of all cats were observed, and any adverse reactions such as aversion, diarrhea, or vomiting were recorded.

[0045] Detection indicators and methods: Feed intake performance testing: The experimental cats were weighed before and after the experiment, while fasting. The food intake and water consumption of the experimental cats were recorded before each morning feeding. At the end of the experiment, the average daily food intake and average daily water consumption of all experimental cats were calculated.

[0046] Oral health examination and testing: After the experiment, all experimental cats underwent oral examinations. The oral health examinations included sensory assessments of gingiva, plaque, tartar, breath, and oral pH.

[0047] Sensory scoring criteria for gums: 0 points - normal gums with sharp, non-inflamed margins; 1 point - marginal gingivitis, with mild inflammation at the free margin that does not extend to the peri-tooth region and there is no bleeding when pressure is applied to the gums; 2 points - moderate gingivitis, pervasive around the teeth, with wider inflammation at the gum margins and bleeding when pressure is applied to the gums; 3 points - significant gingivitis, characterized by marked redness, swelling, bleeding, and a tendency to ulcerate.

[0048] Sensory scoring criteria for dental plaque: 0 points - no dental plaque; 1 point - dental plaque is only present on the interproximal, buccal, or lingual surfaces; 2 points - dental plaque is present on all interproximal, buccal, and lingual surfaces, but covers less than half of these surfaces; 3 points - dental plaque is present on all interproximal, buccal, and lingual surfaces, and covers more than half of these surfaces.

[0049] The sensory evaluation of dental calculus involves observing the state of dental calculus on a pet cat's teeth under natural light, combined with observing the presence of red, lumpy material at the tooth roots under Wood's lamp in a dim light environment.

[0050] The breath sensory evaluation was conducted by four professionals using a double-blind nasal test to assess the odor of the cat's mouth, while a gas analyzer was used to measure the ammonia nitrogen content (in PPM) in the cat's mouth.

[0051] Oral pH measurement: Use a sterile cotton swab to collect secretions from the cat's oral cavity, including the tongue, sublingual area, and area behind the molars. Wipe the cotton swab onto pH test paper to spread the secretions evenly, and immediately compare the reading with the standard colorimetric card.

[0052] Gut health assessment: Before feeding each day, score the appearance of each cat's feces (feces score).

[0053] At the end of the experiment, fresh feces were collected for cryopreservation at -80°C. Samples were taken from four cats in each group, and 16S rDNA metagenomic sequencing was performed using high-throughput sequencing technology. OTUs were clustered with 97% consensus, and species annotation was performed on the OTU sequences. Common and unique OTUs among different sample groups were analyzed, and Venn diagrams were drawn.

[0054] Alpha diversity analysis was performed, including calculations of indices such as Chao1, dominance, goods_coverage, observed_features, pielou_e, Shannon, and Simpson. Goods_coverage represents sample coverage, observed_features and Chao1 indices calculate community abundance, and Shannon, Simpson, dominance, and pielou_e reflect species diversity. NMDS analysis was conducted, and NMDS plots were generated. This, combined with Anosim analysis, determined whether differences in microbial structure existed between groups.

[0055] The top ten most abundant species were selected and their gut microbiota differences at the phylum and genus levels were analyzed using the T-test. LEfSe analysis was used to calculate the LDA values ​​of species, and species with LDA values ​​greater than 2 were selected for differential identification. A bar chart of LDA value distribution and an evolutionary clade diagram were then plotted.

[0056] The ammonia nitrogen content and pH value of feces were measured.

[0057] Experimental results: Feeding performance: As shown in Table 1, with no significant difference in initial body weight, there were no significant differences in final body weight, daily food intake, and daily water intake between the experimental group and the control group (P>0.05).

[0058] Table 1 Results of feeding performance measurement

[0059] The results show that the fresh cat food of the present invention has good palatability and will not have an adverse effect on the cat's food intake and water intake.

[0060] Oral health: As shown in Table 2, there were no significant differences in oral health indicators between the experimental group and the control group of pet cats (P>0.05).

[0061] Table 2 Results of Oral Health Indicators Measurement

[0062] The results show that the fresh cat food of the present invention improves intestinal health without adversely affecting the oral health of cats.

[0063] Gut health: As shown in Table 3, the ammonia nitrogen in the feces of the experimental group was significantly lower than that in the control group (P<0.001), and the fecal score and fecal pH were within the normal range.

[0064] Table 3 Results of fecal index measurements

[0065] The results show that the fresh cat food of the present invention can significantly reduce the ammonia nitrogen content in feces and effectively alleviate fecal odor.

[0066] Gut microbiota: OTUs analysis: The experimental group and the control group contained 111 and 147 OTUs, respectively, with a total of 325 OTUs between the groups.

[0067] α-diversity analysis: As shown in Table 4, the sample coverage was 100%, indicating that the analysis could well represent the situation of each sample, and there was no significant difference in gut microbial α diversity (P>0.05).

[0068] Table 4. Analysis of gut microbial α-diversity

[0069] β-diversity analysis: In PCA analysis of phylum and genus level classification, the different distributions of the two sample points indicate that there are differences in the species composition of the two samples.

[0070] Phylum-level species composition: At the phylum level, the top three most abundant species in the experimental group were Bacteroidetes, Actinobacteria, and Actinobacteria, accounting for over 95% of the total species. In the control group, the top three most abundant species were Firmicutes, Bacteroidetes, and Actinobacteria, accounting for over 79% of the total species. The abundance of Firmicutes in the experimental group was significantly higher than that in the control group (P<0.05), while the abundance of Bacteroidetes, Fusobacteria, and Pseudomonas in the experimental group was significantly lower than that in the control group (P<0.05).

[0071] Genus-level species composition: The levels of *Peptococcus* and *Macrococcus* in the experimental group were significantly higher than those in the control group (P<0.05), while the levels of *Anaerobes* were significantly lower than those in the control group (P<0.05). *Bacteroides* and *Clostridium* showed a significant decreasing trend (P<0.05). <P<0.1)。

[0072] Differential species analysis: In the experimental group, Firmicutes, Broutella, Trichophyceae, Trichophyles, Macrococcus, and Veillonaceae were significantly enriched. In the control group, Prevotellaceae, Fusobacterium, Fusobacteriumceae, Fusobacterium class, and Fusobacteriales were significantly enriched.

[0073] Among them, Broutella spp. are important short-chain fatty acid-producing bacteria that can protect the body's intestinal health and enhance the intestinal barrier function.

[0074] Compared with Examples 1-5 and Comparative Example 1, Examples 1-5 of this invention provide specific formulas and preparation methods for fresh cat food that improves intestinal health and alleviates fecal odor in cats. Example 1 uses 50% fresh chicken and 10% fresh duck as raw materials, combined with 8% chicken liver and 4% chicken heart. 13% of the chicken is enzymatically hydrolyzed using papain at 50°C for 4 hours. It is supplemented with 5% chicken fat, 3% yam, 2% pumpkin, 1% vitamin premix, 1% mineral premix, 0.3% taurine, 0.7% fructooligosaccharides, and 0.5% functional additives (a 1:1 mixture of yucca extract and tea polyphenols). The mixture is then cooked in stages at 82°C / 95°C (total time 23 minutes) to obtain particles with a diameter of 8mm-10mm. Example 2 uses 55% fresh fish instead of poultry, hydrolyzed with trypsin at 48°C for 3 hours, with the functional additive ratio adjusted to 2:1, and cooked at 80°C / 92°C. Example 3 uses a blend of 40% fresh rabbit meat and 20% fresh duck meat, hydrolyzed with a compound protease at 52°C for 5 hours, with an additive ratio of 1.5:1, and a cooking temperature of 85°C / 98°C. Example 4 increases the proportion of fresh chicken meat to 60%, reduces the hydrolyzed meat to 12%, and increases the additive to 0.6%. Example 5 uses a blend of 45% fresh duck meat and 15% fresh fish meat, hydrolyzed with trypsin at 50°C for 4 hours. The raw material ratios in all examples fall within the range defined in claim 1 (55%–65% fresh meat, 8%–15% animal offal, 12%–18% hydrolyzed meat, 3%–6% animal fat, 2%–5% functional plant materials, 0.5%–1.5% vitamin premix, 0.5%–1.5% mineral premix, 0.2%–0.4% taurine, 0.5%–1.2% prebiotics, and 0.3%–0.8% functional additives), and the preparation methods all follow the steps defined in claim 6, with the total cooking time for each segment not exceeding 25 minutes. In Experiment 1, 20 healthy Linqing Lion Cats (weighing 2.93±0.34kg) were randomly divided into an experimental group and a control group, with 10 cats in each group. The experiment lasted for 28 days. The experimental group was fed the product of Example 1 in addition to a basic diet, while the control group was fed only the basic diet. The cats were comprehensively evaluated through feeding performance testing, oral health examination (gingiva, dental plaque, dental calculus, bad breath, oral pH and ammonia concentration), fecal index testing, and 16S rDNA high-throughput sequencing.

[0075] The difference between Comparative Example 1 and Example 1 is that no enzymatically hydrolyzed meat is added, and the amount of fresh chicken is increased to 55%. The other raw materials and preparation methods are the same as those in Example 1. The raw material composition of Comparative Example 2 is exactly the same as that of Example 1. The difference is that in the preparation method, step S5 uses the traditional high-temperature cooking process (cooking at 121°C for 20 minutes) instead of segmented low-temperature slow steaming. The results of Experiment 1 showed that: in terms of feeding performance, there were no significant differences between the experimental group and the control group in initial body weight, final body weight, daily food intake, and daily water intake (P>0.05), indicating that the product of the present invention has good palatability; in terms of oral health, the P values ​​of gingival score (0.80 vs 0.90), dental plaque score (0.60 vs 0.90), dental calculus score (0.70 vs 0.40), halitosis score (0.30 vs 0.40), oral pH (7.45 vs 7.40), and ammonia concentration (1.46ppm vs 1.44ppm) were all greater than 0.05, confirming that the product of the present invention has no adverse effects on oral health while improving intestinal health; in terms of intestinal health, the fecal ammonia nitrogen in the experimental group (59.54mg / g) was reduced by about 35% compared with the control group (91.93mg / g) (P<0.001), and the fecal score and fecal pH were both within the normal range. Microbial analysis showed that the experimental group had significantly higher Firmicutes levels than the control group (P<0.05), while Bacteroidetes, Fusobacterium, and Pseudomonas levels were significantly lower (P<0.05). At the genus level, the experimental group had significantly higher levels of *Macrococcus* (5.75 vs 0.93, P=0.005) and significantly lower levels of *Anaerobes* (0.61 vs 4.27, P=0.028). LEfSe analysis indicated that Firmicutes, *Broutella*, *Trichophyceae*, *Trichophyles*, *Macrococcus*, and *Veillonaceae* were significantly enriched in the experimental group. Comparative Example 1, lacking enzymatically hydrolyzed meat and without pre-digestion of protein, showed a significantly higher fecal ammonia nitrogen level compared to Example 1, and a lower enrichment of beneficial bacteria. This confirms that enzymatically hydrolyzed meat, by breaking down large protein molecules into smaller peptides to improve digestibility and reduce colonic putrefaction and fermentation, is a key component in reducing ammonia nitrogen and regulating gut microbiota. Comparative Example 2, due to the use of high-temperature cooking at 121℃, had its small peptide activity and heat-sensitive nutrients destroyed by enzymatic hydrolysis, resulting in a less effective outcome than Example 1. This confirms that the segmented low-temperature slow steaming process of 80℃~98℃ of the present invention is an important process guarantee for preserving the small peptide activity and nutrients and achieving the above-mentioned technical effects.

[0076] The above are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of protection of the claims.

[0077] In conclusion, the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fresh cat food that simultaneously improves oral and intestinal health in cats, characterized in that, The ingredients include the following percentages by weight: 55%–65% fresh meat, 8%–15% animal offal, 12%–18% enzymatically hydrolyzed meat, 3%–6% animal fat, 2%–5% functional plant ingredients, 0.5%–1.5% vitamin premix, 0.5%–1.5% mineral premix, 0.2%–0.4% taurine, 0.5%–1.2% prebiotics, and 0.3%–0.8% functional additives; the functional additives are a mixture of yucca extract and tea polyphenols.

2. The fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 1, characterized in that, The fresh meat is at least one of chicken, duck, fish, rabbit, and pigeon; the animal offal is at least one of chicken liver, chicken heart, and duck liver.

3. The fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 1, characterized in that, The enzymatically hydrolyzed meat is the product obtained by enzymatically hydrolyzing fresh meat with protease at 45℃~55℃ for 2 to 6 hours.

4. The fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 1, characterized in that, The functional plant material is at least one of yam, pumpkin, and sweet potato; the prebiotic is at least one of fructooligosaccharide, galactooligosaccharide, and inulin.

5. A fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 1, characterized in that, The mass ratio of the yucca extract to tea polyphenols is 1:1 to 2:

1.

6. A method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats, as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Clean the fresh meat and animal offal, remove the fascia and foreign objects, and cut into pieces; S2. Mix some fresh meat with protease and enzymatically hydrolyze at 45℃~55℃ for 2 to 6 hours to obtain enzymatically hydrolyzed meat paste; S3. Mix the pretreated fresh meat, animal offal, enzymatically hydrolyzed meat paste, animal fat, functional plant ingredients, vitamin premix, mineral premix, taurine, prebiotics and functional additives according to the stated mass percentages, and then grind them into a meat paste. S4. The minced meat is extruded into granules, the diameter of which is 6mm to 12mm; S5. The shaped granules are cooked in stages. The first stage is cooked at 80℃~85℃ for 12 minutes~15 minutes, and the second stage is cooked at 92℃~98℃ for 6 minutes~8 minutes. S6. Cool the cured granules to room temperature, vacuum pack or nitrogen-filled pack, and store at 0℃~4℃.

7. The method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 6, characterized in that, The protease mentioned in step S2 is papain or trypsin.

8. The method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 6, characterized in that, The diameter of the particles mentioned in step S4 is 8 mm to 10 mm.

9. The method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats as described in claim 6, characterized in that, The total time for the segmented steaming in step S5 shall not exceed 25 minutes.

10. A method for preparing fresh cat food that simultaneously improves oral and intestinal health in cats, as described in claim 6, characterized in that... The refrigeration temperature described in step S6 is 0℃~4℃.

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