A feed additive for macrobrachium rosenbergii breeding and a preparation method thereof

Abstract

The application discloses a feed additive for Macrobrachium rosenbergii breeding and a preparation method thereof. The feed additive comprises astaxanthin and nicotinamide mononucleotide, and the weight ratio of the astaxanthin to the nicotinamide mononucleotide is 1:0.3-5. The astaxanthin and the nicotinamide mononucleotide are combined to enhance the resistance of Macrobrachium rosenbergii to adverse environment and the stress resistance to algal toxins, avoid metabolic disorder, and promote the healthy growth of the Macrobrachium rosenbergii. The feed additive of the application further comprises black soldier fly alcohol-soluble protein, the black soldier fly alcohol-soluble protein is used to stabilize the emulsification of the fat-soluble astaxanthin in water, and also helps the Macrobrachium rosenbergii to supplement animal protein.

Description

Technical Field

[0001] This invention relates to a feed additive for the farming of giant freshwater prawns, and also to a method for preparing the feed additive. Background Technology

[0002] In recent years, as the consumption of giant freshwater prawns (Macrobrachium rosenbergii) has become increasingly accepted, its farming area has surged and production has steadily increased, but the marketable size remains consistently small. Field investigations frequently reveal varying degrees of cyanobacterial blooms in giant freshwater prawn farming ponds. Many species of cyanobacteria, such as Microcystis, produce microcystin (MCS), which forces prawns into oxidative stress, attacks their hepatopancreatic cells, and causes a significant increase in metabolic energy. Simultaneously, the accumulated algal toxins in the prawns can harm humans through the food chain, reducing the safety of giant freshwater prawns for consumption. Furthermore, compared to the natural diet of giant freshwater prawns, current formulated feeds lack natural insect protein and are nutritionally unbalanced. Additionally, the large number of saprophytic bacteria in the farming water and the lack of beneficial bacteria in the prawns' intestines make them prone to diseases such as enteritis. With increasing awareness of food safety and health, the addition of natural insect protein, beneficial bacteria, and antioxidants has gradually become a focus of healthy and ecological freshwater shrimp farming. Therefore, it is necessary to address these issues through a safe and healthy biological feed for giant freshwater prawns. Summary of the Invention

[0003] Purpose of the invention: The purpose of this invention is to provide a feed additive for giant freshwater prawn farming that can reduce intestinal oxidative stress. This invention also provides a method for preparing this additive, which improves feed activity and stability.

[0004] Technical solution: The feed additive for giant freshwater prawn farming described in this invention includes astaxanthin and nicotinamide mononucleotide, with the weight ratio of astaxanthin to nicotinamide mononucleotide being 1:0.3~5.

[0005] Astaxanthin can quench singlet oxygen, scavenge free radicals, and effectively terminate peroxidation chain reactions in organisms. As an active anti-stress factor, it protects cells from oxidative damage and enhances the organism's stress resistance. In giant freshwater prawns (Macrobrachium rosenbergii), astaxanthin can reduce the accumulation of common algal toxins, improving the food safety of giant freshwater prawns. Nicotinamide mononucleotide (NMN) is a naturally occurring bioactive nucleotide that can rapidly increase the level of an important coenzyme in animal cells—nicotinamide adenine dinucleotide. This invention utilizes the combination of astaxanthin and nicotinamide mononucleotide to enhance the giant freshwater prawn's ability to resist adverse environments and algal toxin stress, prevent metabolic disorders, and promote the healthy growth of giant freshwater prawns.

[0006] Preferably, the weight ratio of astaxanthin to nicotinamide mononucleotide is 1:0.3~2.5.

[0007] Preferably, the feed additive includes black soldier fly larvae prolysin, with a weight ratio of black soldier fly larvae prolysin to astaxanthin of 15-25:1. The black soldier fly (Hermetia illucens), also known as the bright-spotted flat-horned soldier fly, is a novel energy insect rich in protein and oil. Its defatted larval powder has a crude protein content as high as approximately 53%, of which prolysin content is around 21%. Black soldier fly larvae prolysin has strong emulsifying properties (30.5 m...). 2 The foaming property is low (< 8%). Black soldier fly larvae prolysin is used to stabilize and emulsify fat-soluble astaxanthin in water, which also helps to supplement the animal protein of giant freshwater prawns.

[0008] Preferably, the feed additive includes soybean oil, and the ratio of soybean oil to astaxanthin is 0.9~0.95:0.05~0.1.

[0009] Preferably, the feed additive includes a compound bacterial solution, which is a mixture of Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae culture media in a weight ratio of 0.05~0.15: 0.35~0.45: 0.35~0.45: 0.05~0.15. The ratio of black soldier fly larvae prolysin to the compound bacterial solution is 1g: 200~350mL. After entering the shrimp, Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae can adhere to the intestinal wall, excluding the living space of harmful bacteria. Beneficial bacteria, through growth and reproduction, produce lactic acid and acetic acid, which lower the pH of the shrimp's intestine, improve the intestinal microenvironment, and their metabolites promote the shrimp's immune function.

[0010] Preferably, the viable count of Bacillus licheniformis in the compound bacterial solution is 1.5 × 10⁻⁶. 9 CFU / mL ~ 2.0 × 10 9 The CFU / mL concentration of Bacillus subtilis in the solution was 2.0 × 10⁻⁶. 9 CFU / mL ~ 3 × 10 9 The CFU / mL concentration of Lactobacillus acidophilus in the solution was 1.0 × 10⁻⁶. 9 CFU / mL ~ 2.0 × 10 9 The CFU / mL concentration and the viable count of brewer's yeast in the brewer's yeast solution were 2.0 × 10⁻⁶. 9 CFU / mL ~2.5×10 9 CFU / mL.

[0011] Preferably, the feed additive includes trace elements, namely iron, manganese, selenium, and calcium, which are dissolved in the compound bacterial solution, with iron content of 3-5 mg / mL, manganese content of 3-6 mg / mL, selenium content of 0.8-1 mg / mL, and calcium content of 18-22 mg / mL.

[0012] Preferably, the feed additive includes a preservative, wherein the preservative is sodium D-isoascorbate, and the weight ratio of black soldier fly larvae prolysin to the preservative is 1:0.2~0.25.

[0013] Preferably, the feed additive includes sodium chloride, and the weight ratio of black soldier fly larvae prolysin to sodium chloride is 1:0.2~0.25.

[0014] Preferably, the feed additive is added at a ratio of 10-40% by weight. After adding the feed additive, the final astaxanthin content in the feed can be 8-150 mg / kg. More preferably, the feed additive is added at a ratio of 10-30%. Astaxanthin is a fat-soluble substance and requires the assistance of oils such as soybean oil to increase its dispersion. The amount added should not be too high, otherwise it will result in a high oil content in the feed or poor dispersion, making it unabsorbable by shrimp. After adding the feed additive, the final astaxanthin content in the feed is preferably 10-60 mg / kg.

[0015] The aforementioned method for preparing feed additives for giant freshwater prawn farming comprises the following steps:

[0016] The first compound bacterial solution was extracted with ethyl acetate to obtain a compound bacterial solution extract. The compound bacterial solution extract was mixed with 3-8 wt% sodium alginate solution at a ratio of 1 g: 8-12 mL to form the first component.

[0017] Mix black soldier fly larvae prolysin and astaxanthin, add water at 8-12 times the total weight of black soldier fly larvae prolysin and astaxanthin to emulsify the astaxanthin, and continue to add nicotinamide mononucleotide to obtain an emulsified mixture. Add the emulsified mixture to the second compound bacterial solution at a ratio of 80-120 mg / mL to form the second component.

[0018] The first and second components are mixed and then added to an 8-12 wt% calcium lactate-chitosan mixed solution for granulation. Granulation is used to improve the viability of live bacteria.

[0019] Preferably, the weight ratio of the first component to the second component is 1:1 to 5. More preferably, the weight ratio of the first component to the second component is 1:1.5 to 4. Most preferably, the weight ratio of the first component to the second component is 1:1.5 to 2.5.

[0020] The ethyl acetate extract of Bacillus licheniformis fermentation broth can be used as a bacterial quorum sensing inhibitor, also known as a qs inhibitor (qSIS). It interferes with the bacterial quorum sensing process by inhibiting the biosynthesis of bacterial quorum sensing signal molecules, degrading these molecules, competing with them for receptor sites, or binding to and clearing them, thereby reducing the pathogenicity and drug resistance of pathogenic bacteria. The granulation process requires water, and the compound bacterial solution is easily diluted. A portion of the compound bacterial solution is first dehydrated and then mixed with sodium alginate solution to maintain the concentration and pH of the compound bacterial solution, ensuring sufficient effective concentration and activity. Using calcium lactate for granulation further facilitates feeding, transportation, and storage.

[0021] In the aforementioned preparation method, Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae are cultured at appropriate temperatures according to the culture methods for each type of bacteria. Culture can be carried out in glass Erlenmeyer flasks or aseptic polyethylene culture bags. During the culture process, care should be taken to prevent contamination by other microorganisms. The number of active bacteria in each bacterial solution in step (2) is detected using the plate coating method. After the culture is completed, each bacterial solution is removed from the incubator and left to stand for use. The standby time should not exceed 72 hours.

[0022] In the aforementioned preparation method, black soldier fly larvae prolysin can be commercially available or prepared in-house. The preparation method is as follows: Healthy black soldier fly larvae aged 15-20 days are washed, dried, crushed, and defatted, then passed through a 50-mesh sieve to obtain defatted larvae powder. The defatted larvae powder is added to purified water at a liquid-to-solid ratio of 20 mL:1 g and mixed thoroughly. The mixture is then reacted in a 45°C constant temperature water bath with shaking for 3 hours. Subsequently, the mixture is poured into a centrifuge and centrifuged at 6000 rpm for 20 minutes, collecting the lower layer of crude protein precipitate. The crude protein precipitate is dissolved in 70% ethanol solution at a liquid-to-solid ratio of 10:1 (mL:g), reacted in a 50°C constant temperature water bath with shaking for 2 hours, followed by centrifugation at 4000-6000 rpm. The collected supernatant is the black soldier fly larvae prolysin solution. The prolysin solution is then freeze-dried in a rotary evaporator to obtain black soldier fly larvae prolysin powder. This protein is stored at 4°C for later use.

[0023] In the aforementioned preparation method, the extraction method of the compound bacterial liquid extract is as follows: take the first compound bacterial liquid, centrifuge at 4000~6000 rpm, take the supernatant, and filter it with a 0.22μm cellulose acetate microporous membrane; take the filtrate and add ethyl acetate at a volume ratio of 1:2.5~3.5 into a separatory funnel, and extract by shaking under low temperature conditions (around 4℃). After the separation is completed, collect the upper emulsion solution, remove the ethyl acetate solvent in the emulsion solution by vacuum rotary evaporator, concentrate the remaining part into a paste, and then freeze-dry it under vacuum to prepare the compound bacterial liquid fermentation product ethyl acetate extract powder.

[0024] In the aforementioned preparation method, trace elements are added to the second compound bacterial solution before astaxanthin, and preservatives and sodium chloride are added to the second compound bacterial solution after astaxanthin.

[0025] Beneficial effects: 1. The feed additive of this invention, with the combination of astaxanthin and NMN, reduces intestinal oxidative stress: Astaxanthin reduces the burden on the intestines at the source, thus saving NAD. + The ineffective depletion of NAD+ by NMN leads to the loss of NAD+. + 1. It can be used more effectively for energy production; 2. Insect protein not only serves as an emulsifier but also broadens the nutritional sources of giant freshwater prawns; 3. A reasonable combination of beneficial bacteria can effectively inhibit the growth and reproduction of harmful bacteria in the shrimp's intestines; 4. The feed additives prepared by the method of this invention have high activity and stability, and are easy to feed, transport and store. Attached Figure Description

[0026] Figure 1 A bar chart showing the effect of adding different proportions of Egyptian microcystin on the accumulation of microcystin in the hepatopancreas of Macrobrachium rosenbergii. Detailed Implementation

[0027] The present invention will be further described below with reference to the embodiments and accompanying drawings.

[0028] Example 1: Analysis of the effects of astaxanthin and NMN on intestinal health and growth of giant freshwater prawns.

[0029] Experimental design: Four treatment groups were set up, with three replicates in each group, and the rearing period was 30 days. Fifty healthy giant freshwater prawns of the same initial size (initial weight 0.51±0.11g, initial body length 2.3±0.1cm) were introduced into each group. The rearing conditions were kept consistent throughout the process (water temperature 28±1℃, pH 7.5±0.3, dissolved oxygen ≥6mg / L) to ensure the reliability of the experimental results.

[0030] The groups are as follows:

[0031] Control group (CK): fed with basal feed as a blank control.

[0032] Astaxanthin group (A): 50 mg / kg astaxanthin was added to the basal feed.

[0033] NMN group (N): 100 mg / kg NMN was added to the basal diet.

[0034] Combined group (A+N): 50 mg / kg astaxanthin and 100 mg / kg NMN were added to the basal feed.

[0035] After the experimental period ended, the growth indicators (final body weight, weight gain rate, specific growth rate) and intestinal physicochemical indicators (intestinal villus height, intestinal mucosal thickness, total intestinal antioxidant capacity T-AOC, and intestinal malondialdehyde (MDA) content) of each group of giant freshwater prawns were measured. The test results are shown in Tables 1-3.

[0036] As shown in Table 1 below, the final body weight (2.30±0.13g), weight gain rate (360.00±8.56%), and specific growth rate (4.42±0.19% / d) of the combined group of giant freshwater prawns were significantly higher than those of the control group (1.20±0.07g, 138.00±5.98%, 2.38±0.11% / d) and the two individual treatment groups (astaxanthin group: 1.52±0.08g, 202.00±6.89%, 3.05±0.14% / d; NMN group: 1.56±0.09g, 214.00±6.72%, 3.11±0.13% / d).

[0037] Table 1. Effects of astaxanthin and NMN on growth indicators of giant freshwater prawns.

[0038]

[0039] Table 2. Effects of astaxanthin and NMN on intestinal physicochemical parameters of giant freshwater prawns.

[0040]

[0041] To further confirm whether astaxanthin and NMN have a synergistic effect, a two-way ANOVA was used for statistical analysis. Astaxanthin (A) and NMN (N) were the two independent variables, and various growth and intestinal physicochemical indicators were the dependent variables. The main effects (individual effects) and interaction effects (combined effects) of the two were analyzed. The statistical criteria were: P < 0.05 was considered statistically significant, and P < 0.01 was considered extremely significant. The specific analysis results are shown in Table 3 below.

[0042] Table 3. Analysis of the interaction effects of astaxanthin and NMN on the growth and intestinal physicochemical indicators of giant freshwater prawns.

[0043]

[0044] Example 2 The preparation method of the feed additive in this example is as follows:

[0045] (1) Preparation of black soldier fly larvae prolysin: Healthy black soldier fly larvae aged 15-20 days, fed with wheat bran or kitchen waste, were isolated, washed, dried, pulverized, and defatted using a Soxhlet extractor and ether solution at room temperature. After defatting, the larvae were pulverized again using a mixer and passed through a 50-mesh sieve to obtain defatted black soldier fly powder. The defatted powder was added to purified water at a liquid-to-solid ratio of 20 mL:1 g and mixed well. After shaking and reacting for 3 hours in a 45°C constant temperature water bath, the mixture was centrifuged for 20 minutes, and the lower precipitate was collected. The precipitate was then dissolved in 70% ethanol solution at a liquid-to-solid ratio of 10 mL:1 g, and the solution was incubated in a 50°C constant temperature water bath for 2 hours, followed by centrifugation at 6000 rpm. The supernatant was collected. The supernatant was freeze-dried using a freeze dryer to obtain a light yellow black soldier fly prolysin powder, which was stored in a 4°C refrigerator for later use.

[0046] (2) Extract Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae strains from the culture room and incubate them at a suitable temperature. Culture can be done in glass Erlenmeyer flasks or aseptic polyethylene culture bags. During the incubation process, take precautions to prevent contamination by other microorganisms. Detect the number of viable bacteria in each culture using the plate spread method, ensuring the viable count is not less than 1.0 × 10⁻⁶. 9 CFU / mL, remove each bacterial culture from the incubator and wait for use. The waiting time should not exceed 72 hours.

[0047] (3) The Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus and Saccharomyces cerevisiae obtained in step (2) are mixed in a weight ratio of 0.1:0.4:0.4:0.1 to prepare a composite microbial culture, which is then stored at 4°C for later use.

[0048] (4) Divide the composite microbial culture in step (3) into two parts, A and B. Place part B into a centrifuge and centrifuge at 6000 rpm. Filter the supernatant after centrifugation with a 0.22 μm cellulose acetate microporous membrane. Add the filtrate and ethyl acetate in a volume ratio of 1:3 into a separatory funnel and extract by shaking at a low temperature (around 4°C). After the separation is completed, collect the upper emulsion solution and remove the ethyl acetate solvent in the emulsion solution by vacuum rotary evaporator. Concentrate the remaining part into a paste and then freeze-dry it under vacuum to prepare a milky white composite microbial culture fermentation product ethyl acetate extract powder. Mix the ethyl acetate extract powder with 5% sodium alginate solution at a material-liquid ratio of 1 g:10 mL to form an ethyl acetate extract-sodium alginate mixture.

[0049] (5) Weigh the corresponding masses of FeCl2, MnSO4, sodium selenite and CaCl2, add them to the compound bacterial solution A, and mix them with a magnetic stirrer at 4℃ to make the Fe content 6mg / mL, Mn content 7.5mg / mL, selenium content 1.2mg / mL and Ca content 30mg / mL.

[0050] (6) Take astaxanthin capsules (effective astaxanthin content is about 5%, and the excipient is soybean oil), cut them open and squeeze out the clear oily contents. Mix them with the black soldier fly alcohol-soluble protein powder prepared in step (1) at a weight ratio of 1:1. Then mix the mixture with 10 times the mass of pure water and treat it with an ultrasonic mixer for 2 to 4 minutes. The astaxanthin emulsified by the alcohol-soluble protein can be uniformly dissolved in the liquid. After ultrasonic treatment, the substance is an emulsion. Then add 0.1% of the total mass of the solution of nicotinamide mononucleotide (effective content ≥95%), 1% of D-isoascorbic acid sodium salt and 1% of NaCl, mix well and store at 4°C for later use. The weight ratio of astaxanthin to nicotinamide mononucleotide is about 1:0.44.

[0051] (7) Take the emulsified astaxanthin-prolyzed protein mixture prepared in step (6), and add the mixture to the composite bacterial solution prepared in step (5) at a ratio of 100 mg / mL. Then continue to treat the solution with an ultrasonic mixer for 5 minutes to maintain the overall state of a uniform emulsion.

[0052] (8) Mix the emulsified liquid-like composite microbial culture prepared in step (7) with the ethyl acetate extract-sodium alginate mixture at a mass ratio of 2:1. Mix thoroughly with a magnetic stirrer at 4°C. Drop the mixture through a porous circular sieve with a pore size of 1.5–2.0 mm into a liquid mixture of 10% chitosan and calcium lactate (chitosan:calcium lactate:purified water = 5:5:90 by mass). The liquid sodium alginate solidifies upon contact with chitosan and calcium lactate to form a calcium alginate-chitosan mixture, which then forms gelatinous particles with a diameter of approximately 1.5–2.0 mm. These particles can be directly fed to giant freshwater prawns or sealed and stored at 4°C for a long period of approximately 30 days.

[0053] The feed additive prepared in Example 2 was used to test its effects on animal husbandry.

[0054] Sixteen culture tanks, each measuring 400 × 500 × 500 mm, were prepared. 160 giant freshwater prawn larvae (approximately 15.12 ± 1.37 g) were divided into two groups, with eight replicates per group and ten larvae per replicate. One group was fed a formulated giant freshwater prawn feed, while the other group was fed a mixture of 70% formulated giant freshwater prawn feed and 30% of the feed additive prepared in Example 2. The tanks were disinfected with povidone-iodine before the experiment.

[0055] During the rearing process, aeration was maintained 24 hours a day, and uneaten feed and feces were promptly removed to prevent water quality deterioration. The experiment lasted for 21 days, with 1 / 5 of the water changed daily and the shrimp fed three times a day. The daily feed amount was approximately 2% of the shrimp's body weight, and adjustments were made based on their feeding behavior. After 21 days, samples were collected, weighed, and analyzed.

[0056] As shown in Table 4, adding the invented feed additive during the cultivation of giant freshwater prawns can significantly promote the growth of giant freshwater prawns (p < 0.05).

[0057] Table 4. Effects of bio-feed on the growth of giant freshwater prawns

[0058]

[0059] Example 3: Effects of different proportions of feed additives on the hepatopancreas

[0060] In this embodiment, the grouping and rearing steps are the same as in Example 2. One group was fed 80% Giant River Prawn formulated feed + 20% feed additive prepared in Example 2, and the other group was fed 90% Giant River Prawn formulated feed + 10% feed additive prepared in Example 2. The accumulation of microcystin MC-LR in the pancreas of the shrimp in Example 2 and this embodiment was tested (measured using an ELISA kit from Beijing Jinqiang Environmental Protection Co., Ltd.). The test results are as follows: Figure 1 As shown (*different letters above the data indicate significant differences, P < 0.05), the results show that replacing part of the ordinary feed with the additive of the present invention can significantly reduce the accumulation of microcystin MC-LR in the hepatopancreas of giant freshwater prawns.

Claims

1. A feed additive for giant freshwater prawn farming, characterized in that, It includes astaxanthin and nicotinamide mononucleotide, with a weight ratio of astaxanthin to nicotinamide mononucleotide of 1:0.3~5.

2. The feed additive for giant freshwater prawn farming according to claim 1, characterized in that, The feed additive includes black soldier fly larvae prolysin, with a weight ratio of black soldier fly larvae prolysin to astaxanthin of 15-25:

1.

3. The feed additive for giant freshwater prawn farming according to claim 1, characterized in that, The feed additive includes soybean oil, and the ratio of soybean oil to astaxanthin is 0.9~0.95:0.05~0.

1.

4. The feed additive for giant freshwater prawn farming according to any one of claims 1 to 3, characterized in that, The additive includes a compound bacterial solution, which is a mixture of Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae culture media in a weight ratio of 0.05~0.15: 0.35~0.45: 0.35~0.45: 0.05~0.

15. The ratio of black soldier fly larvae prolysin to the compound bacterial solution is 1g: 200~350mL.

5. The feed additive for giant freshwater prawn farming according to claim 4, characterized in that, The composite bacterial solution contained 1.5 × 10⁻⁶ live Bacillus licheniformis bacteria. 9 CFU / mL ~ 2.0 × 10 9 The CFU / mL concentration of Bacillus subtilis in the solution was 2.0 × 10⁻⁶. 9 CFU / mL ~ 3 × 10 9 The CFU / mL concentration of Lactobacillus acidophilus in the solution was 1.0 × 10⁻⁶. 9 CFU / mL ~ 2.0 × 10 9 The CFU / mL concentration and the viable count of brewer's yeast in the brewer's yeast solution were 2.0 × 10⁻⁶. 9 CFU / mL ~2.5×10 9 CFU / mL.

6. The feed additive for giant freshwater prawn farming according to claim 4, characterized in that, The feed additive includes trace elements, namely iron, manganese, selenium, and calcium. The trace elements are dissolved in the compound bacterial solution, with iron content of 3-5 mg / mL, manganese content of 3-6 mg / mL, selenium content of 0.8-1 mg / mL, and calcium content of 18-22 mg / mL.

7. The feed additive for giant freshwater prawn farming according to claim 4, characterized in that, The feed additive includes a preservative, which is sodium D-isoascorbate, and the weight ratio of black soldier fly larvae prolysin to the preservative is 1:0.2~0.

25.

8. The feed additive for giant freshwater prawn farming according to claim 4, characterized in that, The feed additive is added at a rate of 10-40% by weight.

9. The method for preparing feed additives for giant freshwater prawn farming as described in claim 5, characterized in that, The steps are as follows: The first compound bacterial solution was extracted with ethyl acetate to obtain a compound bacterial solution extract. The compound bacterial solution extract was mixed with 3-8 wt% sodium alginate solution at a ratio of 1 g: 8-12 mL to form the first component. Mix black soldier fly larvae prolysin and astaxanthin, add water at 8-12 times the total weight of black soldier fly larvae prolysin and astaxanthin to emulsify the astaxanthin, and then add nicotinamide mononucleotide to obtain an emulsified mixture; add the emulsified mixture to the second compound bacterial solution at a ratio of 80-120 mg / mL to form the second component; The first and second components were added to an 8-12 wt% calcium lactate-chitosan mixed solution for granulation.

10. The preparation method according to claim 9, characterized in that, The weight ratio of the first component to the second component is 1:1 to 5.

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