Bletilla striata root composite solid beverage for relieving ulcerative colitis

Abstract

The application discloses a kind of medicine and food homologous herbal functional composite solid beverage, belong to functional food processing technical field.The product with the high activity E-SDF prepared by composite enzyme method as main functional component, compound hawthorn powder, erythritol and malt dextrin, by single factor test and Box-Behnken response surface method optimization formula, wherein E-SDF, hawthorn powder, erythritol and malt dextrin mass fraction is 40%, 14%, 13.5% and 32.5% respectively.Product is screened by raw material, uniformly mixed and is made by subpackaging.The application improves the pure white and must dietary fiber taste bitter, the problem such as easy to be agglomerated and layered in brewing, and long-term food compliance is poor, the product obtained has good brewing performance, flavor coordination, can be fully dissolved in a short time, physical and chemical and microbiological indexes meet relevant food safety standards, and has good storage stability.The product is rich in high-activity soluble dietary fiber, has the application potential of regulating intestinal microecology and maintaining intestinal health, and realizes the resource high-value utilization of white and processing byproduct root.

Description

Technical Field

[0001] This invention belongs to the field of food and medicine homology herbal solid beverage, intestinal functional instant food, and dietary intervention food processing technology for ulcerative colitis. Background Technology

[0002] Existing solid beverages for gut health have several drawbacks: most commercially available solid beverages for gut health consist of only one type of dietary fiber and are not designed for the entire treatment cycle of ulcerative colitis (UC) from acute to recovery; the powder of simple plant-based dietary fiber has a rough texture and a noticeable herbal, fishy taste, and it is very easy for the product to clump and settle when mixed with water, making it difficult for consumers to drink it consistently over a long period; there is a lack of dedicated UC beverages that take into account flavor, solubility, and anti-inflammatory and repairing effects on the gut.

[0003] Raw material technical description: Soluble dietary fiber (SDF) in the fibrous roots of Bletilla striata, a by-product of processing, has intestinal conditioning activity; E-SDF prepared by compound enzyme method has the functions of inhibiting inflammatory pathways, repairing intestinal mucosa, and regulating intestinal flora. It is the core functional raw material of this product. However, pure E-SDF powder has poor palatability and is difficult to use directly as a final beverage.

[0004] Necessity of developing excipients: Hawthorn powder can neutralize the fishy and astringent taste of fiber and impart a natural sweet and sour fruity aroma; erythritol is a zero-burden sweetener that does not raise blood sugar; maltodextrin improves the flowability and reconstitution properties of powder. The combination of the three can perfectly balance function and eating experience. Summary of the Invention

[0005] The technical problems to be solved by this invention are: overcoming the defects of pure E-SDF powder such as bitterness, clumping, poor solubility, and poor palatability, and providing a compound solid beverage formula that takes into account sensory appeal, solubility, and intestinal conditioning effects; standardizing the powder mixing and individual sachet packaging process, ensuring that the product's physicochemical and microbiological indicators all meet national standards, with a long shelf life, portability, and easy storage; and developing a daily instant dietary beverage that can be used simultaneously for the relief of acute inflammation in UC and intestinal care during the recovery period, realizing the end-product food utilization of waste Bletilla striata roots.

[0006] The optimal formula for this product is: 40% soluble dietary fiber E-SDF from Bletilla striata rootlets prepared by compound enzyme method, 14% hawthorn powder, 13.5% erythritol, and 32.5% maltodextrin. Among them, E-SDF is prepared by compound enzyme process, with a purity of ≥67.50%, microscopic loose and porous structure, water holding capacity ≥5.89 g / g, and has the biological activity of inhibiting the NF-κB / NLRP3 inflammatory pathway, reducing pro-inflammatory factors, and repairing colonic erosion mucosa.

[0007] Standardized and complete preparation process: S1 Raw material pretreatment: E-SDF, hawthorn powder, erythritol, and maltodextrin are each individually sieved through a 60-mesh standard sieve to remove coarse particles and clumps, ensuring uniform powder particle size; S2 Precise compounding and mixing: Each raw material is precisely weighed according to the optimal formula and put into a three-dimensional motion mixer for uniform stirring for 20 minutes, resulting in powder without layering and with uniform color; S3 Quantitative and independent packaging: Food-grade composite oxygen barrier film strip bags are used, with 3 g of mixture packaged in each bag and heat-sealed; S4 Outer box packaging: 20 strip bags are placed in a printed outer packaging box, with the box fully labeled with ingredients, nutrition label, consumption method, storage conditions, and production qualifications; S5 Finished product inspection and warehousing: Sensory evaluation, moisture, nutrition, total bacterial count, mold, and coliform bacteria are tested sequentially, and the product is stored in a cool, dark place after all tests are passed.

[0008] Product specifications for sensory, physicochemical, and microbiological indicators: Sensory indicators: The powder is uniformly light brown, without mold spots or burnt discoloration; it dissolves completely after stirring in 100 mL of room temperature / warm water for 30 seconds, and shows no stratification or sedimentation after standing for 30 minutes; it has a combination of hawthorn fruit aroma and mild Bletilla striata herbal aroma, with a harmonious sweet and sour taste, and no fishy, ​​astringent, or bitter taste; the overall sensory score can reach 91 points or above; Physicochemical indicators (per 100 g): Moisture 5.80±0.07 g (≤7%, meeting the limit of GB / T 29602); Fat 0.50 g, Carbohydrates 90.4 g, Protein 1.70 g, Sodium 671 mg; Low in fat and carbohydrates, suitable for the dietary needs of UC populations; Nutrition label energy 1566 kJ / 100g; Microbiological safety indicators: Total bacterial count ≤3.1×10³ CFU / g, mold <10 CFU / g, coliform bacteria <10 CFU, all complying with the National Food Safety Standard and GB / T 29602 Solid Beverage Standard.

[0009] Packaging and Storage Specifications: Inner Packaging: 3g individually wrapped oxygen-barrier composite food bag, isolating moisture and oxygen to prevent powder from absorbing moisture and clumping; Outer Packaging: Light beige + light brown plant-themed cardboard box with complete pre-packaged label printed on it; Storage Conditions: Store in a sealed, dark, cool, and dry place. Shelf life: 3 months; Directions for Use: Take 1 bag (3g) each time, add 100~150 mL of warm water, 1~2 times a day. It can be used as a daily dietary beverage for people with UC.

[0010] (vi) Product efficacy and target audience This product, relying on highly active E-SDF, can achieve a dual conditioning effect: Acute phase: Inhibits pro-inflammatory factors such as TNF-α, IL-6, and IL-1β, repairs colonic erosion, and relieves abdominal pain, mucus and bloody stools, and diarrhea; Recovery period: Continuously supply short-chain fatty acids to regulate beneficial gut flora and reduce the probability of recurrence; Suitable for: People with mild to moderate ulcerative colitis in the acute phase or during the recovery period after inflammation subsides; suitable for long-term daily dietary intervention.

[0011] (vii) Core beneficial effects of the invention Synergistic effects of the formula: Hawthorn powder masks the fishy and astringent taste of fiber, erythritol provides a mild flavor without raising blood sugar, and maltodextrin significantly improves reconstitution properties, solving the problems of pure dietary fiber drinks being difficult to drink and prone to sedimentation; Full-cycle adaptability: The core ingredient E-SDF has dual activity of anti-inflammatory in the acute phase and intestinal care in the recovery phase. One product covers the entire course of UC and has a wider range of application scenarios; Safety and compliance: All raw materials are food-grade, meet the standards for physicochemical and microbiological indicators, are low in fat and sodium, and contain no preservatives. They can be consumed daily for a long time. Portable packaging: 3g individual single-use sachets, convenient to carry when going out, allowing for measured consumption and preventing the ingredients from becoming damp and ineffective; Resource utilization: Using waste roots from Bletilla striata processing as the core raw material, a circular economy is adopted to reduce the production cost of functional foods. Attached Figure Description

[0012] Figure 1 The complete packaging for Bletilla striata root compound solid beverage (outer box + individual strip bag).

[0013] Figure 2 The sensory evaluation curves for the single-factor formulas are as follows: (a) E-SDF addition (%); (b) Hawthorn powder addition (%); (c) Erythritol addition (%); (d) Maltodextrin addition (%).

[0014] Figure 3 Three-dimensional surface plots of the formulation response surface ((a) hawthorn powder with E-SDF; (b) erythritol with E-SDF; (c) hawthorn powder with erythritol).

[0015] Figure 4 The graph shows the changes in body weight and disease activity index of mice in different treatment groups.

[0016] Figure 5 Bar chart of intestinal SCFAs content. Detailed Implementation

[0017] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0018] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0019] Example 1 (Optimal Protected Formula of the Invention): Raw Material Ratio (Total 1000 g): E-SDF 400 g, Hawthorn Powder 140 g, Erythritol 135 g, Maltodextrin 325 g; E-SDF Raw Material Source: Prepared using a compound enzyme process, purity 67.41%, water holding capacity 5.91 g / g; Preparation Steps: ① All four raw materials are individually sieved through a 60-mesh sieve to remove lumps and coarse powder; ② Accurately weigh and add to a three-dimensional mixer, mix at a uniform speed for 20 min; ③ Automatically dispense 3 g / strip, heat-seal; 20 strips are packaged into the finished product box; Finished Product Test Results: Sensory: Light brown uniform powder, completely dissolved in warm water for 30 seconds, sweet and sour taste with no astringency, sensory score 91.2 points; Physicochemical: Moisture 5.78 g / 100g, carbohydrates 90.37 g / 100g; Microbiological: Total colony count 2.1×10³ CFU / g, no detectable mold, coliform bacteria <10 CFU, all met the standards; Efficacy verification: DSS induced UC mice were administered the corresponding dose by gavage daily for 14 consecutive days. The mice regained their body weight, the DAI disease index decreased significantly, the colon length recovered by more than 10%, serum TNF-α, IL-6, and IL-1β decreased significantly, the content of beneficial bacteria and short-chain fatty acids in the cecum increased, and acute hematochezia, diarrhea and intestinal weakness during the recovery period were all improved.

[0020] Example 2: Formula: E-SDF 30%, hawthorn powder 14%, erythritol 13.5%, maltodextrin 42.5%; Preparation process and E-SDF raw materials are the same as in Example 1; Finished product effect: Sensory score 85.7 points, herbal flavor is lighter, the content of dietary fiber functional components is reduced, the effect of relieving UC inflammation and repairing mucosa is significantly weaker than in Example 1, and the physicochemical and microbiological indicators are qualified.

[0021] Example 3: Formula: E-SDF 50%, hawthorn powder 14%, erythritol 13.5%, maltodextrin 22.5%; Preparation process is the same as in Example 1; Finished product effect: After mixing, obvious fine sedimentation appeared, the herbal astringent taste was prominent, the sensory score was only 83.1 points, and the long-term acceptance of users was low; the microbial indicators were qualified, the anti-inflammatory effect was slightly improved, but the palatability was seriously defective.

[0022] Comparative Example: Comparative Formula: 40% E-SDF, 60% maltodextrin, no added hawthorn powder or erythritol; the preparation process is completely consistent with Example 1; product defects: it is easy to clump together when mixed, and after drinking, it has a distinct whitish, astringent, and bitter taste, and the sweet and sour flavor is completely missing. The sensory score is only 69 points, which is difficult for ordinary people to drink for a long time and cannot meet the daily dietary intervention needs of the UC population.

[0023] Summary of Implementation Results: Implementation 1: The optimal formula balances intestinal conditioning efficacy, reconstitution properties, and sensory flavor, defining the core solution for the claims; Too low an E-SDF addition will significantly weaken the product's anti-inflammatory and repairing effects, while too high an addition will result in prominent defects in reconstitution and taste; Hawthorn powder and erythritol are indispensable excipients, and their absence will directly cause the product's palatability to be compromised, making it impossible to achieve the product positioning of long-term dietary intervention.

Claims

1. A compound solid beverage containing Bletilla striata rootlets for relieving ulcerative colitis, characterized in that, The product is composed of the following raw materials by weight percentage: 40% E-SDF soluble dietary fiber from Bletilla striata rootlets prepared by compound enzyme method, 14% hawthorn powder, 13.5% erythritol, and 32.5% maltodextrin. The E-SDF is prepared by compound enzyme extraction process, with a purity ≥67.50%, microscopic loose and porous structure, and a water holding capacity ≥5.89 g / g. It can block the NF-κB and NLRP3 inflammatory pathways, reduce serum pro-inflammatory factors, and repair colonic mucosal erosion.

2. The Bletilla striata root compound solid beverage for relieving ulcerative colitis according to claim 1, characterized in that, Product physicochemical indicators: moisture 5.80±0.07 g / 100g, fat 0.50 g / 100g, carbohydrates 90.4 g / 100g; microbiological indicators: total bacterial count ≤3.1×10³ CFU / g, mold <10 CFU / g, coliform bacteria <10 CFU, all complying with GB / T 29602 "Solid Beverages" national standard.

3. A compound solid beverage containing Bletilla striata rootlets for relieving ulcerative colitis according to claim 1, characterized in that... Sensory characteristics: The powder is uniformly light brown with no discolored mold spots; it dissolves completely when mixed with 100 mL of cold or warm water and stirred for 30 s; there is no stratification or sedimentation after standing for 30 min; the hawthorn fruit aroma is harmonious with the herbal scent, without any astringent, fishy or bitter taste, and the overall sensory score is ≥91 points.

4. A method for preparing the Bletilla striata root compound solid beverage according to any one of claims 1 to 3, characterized in that, The process includes the following steps: S1 Raw material pretreatment: E-SDF, hawthorn powder, erythritol, and maltodextrin are sieved through a 60-mesh sieve to remove coarse particles and lumps; S2 Uniform mixing: All raw materials are accurately weighed according to the formula ratio and put into a three-dimensional mixer and stirred for 20 minutes until the powder is uniform in color and free of layering; S3 Individual packaging: 3 g of the mixture is heat-sealed in oxygen-barrier food-grade bags; S4 Outer packaging: 20 bags are placed in a printed outer packaging box with complete labeling of ingredients, nutrition label, consumption and storage information; S5 Finished product testing: Sensory, moisture, nutritional, and microbiological indicators are tested in sequence. After passing the tests, the product is stored in a cool, dark place with a shelf life of 3 months.

5. The composite solid beverage according to claim 1, characterized in that... Used to prepare general dietary supplements for the acute phase and recovery phase of ulcerative colitis; Directions for use: 1 sachet each time, mixed with 100-150 mL of warm water, 1-2 times daily. Long-term consumption can help reduce intestinal inflammation, repair intestinal mucosa, and regulate intestinal flora.

6. The preparation method according to claim 4, characterized in that... The outer packaging box features a light beige and light brown plant-themed design, while the inner bag is made of multi-layer oxygen-barrier composite food film. The nutrition label per 100g of product indicates: energy 1566kJ, protein 1.7g, fat 0g, carbohydrates 90.4g, and sodium 671mg, which complies with GB 28050 prepackaged nutrition labeling standards.

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